                         SEQUENCE LISTING

<110>  BASF Plant Science GmbH
 
<120>  Expression cassettes for seed-preferential expression in plants

<130>  AE20050149 / PF 56778 EP

<160>  24    

<170>  PatentIn version 3.3

<210>  1
<211>  1602
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  promoter
<222>  (1)..(1602)
<223>  transcription regulating nucleotide sequence from Arabidopsis 
       thaliana gene At2g34700 (putative proline-rich glycoprotein) 
       comprising 5-untranslated region

<220>
<221>  TATA_signal
<222>  (1540)..(1547)
<223>  putative TATA-box

<220>
<221>  5'UTR
<222>  (1573)..(1602)
<223>  putative 5-untranslated region

<400>  1
acattttacg gcactttctt gatcctcata ccatcatata tgtacacatg tatttagtga     60

atcaaaacta ttaaactatt ttaacatttt tcttctaaaa ttaataatta atttctaaat    120

ttccttaatt attgtacaaa aaattaaaag caataatttt ttaaaaattt taaatttcat    180

taattatatg atgaagttta taatttgttt tatttttttt ttttaattac aagatatata    240

tagtttcttt atgtttaact ttttttatat ttaatatttt cttaattcaa aacaaaaaaa    300

aatatatttt aatttttagt ttatttattt atttataaga tactaatagt ttctttatat    360

ttatcgtctt tacttttcaa tcctttttaa tttttatttt tcaaattata agagacataa    420

ctttttaata tttatcttct ttacttttta atcataaata ttttatgata aattaattaa    480

attatgtatt ggttgtttta tgaagtctag gtggcaagct attagatgct aacgtatcaa    540

gctgtgagaa agagttaagc tcctttcata tatataatac aactaaaaat ttgaatcgtc    600

aaagaaaaat acctttaaaa cagaaatttt ctgattttaa aagtgcaaaa aggttagtaa    660

tgccgaattt taacggaggc tattttgtcc aaaaaagatt gttaactagc gatgactatg    720

aggcttgaga ttcttcacca ataaatgccg actcgcttgg attagggata tatttcatca    780

agatacgtga ttactggtgc ccatgcattt acatagtaac cttacattgt taaatttaac    840

attttgtcac taggtaatct tacctaagaa aattaaaaat taaaaatatt atattttttt    900

gttgaataag taacacaact tgtcatgatg ggactatatc attaacacaa cttgaagcac    960

caaccacttt aattaattta tagcctcttc cctccatggc tctatcacca taagaaaact   1020

ttaaggtcat gtctttggct tgcacaatcc attgacaata gtattactat atatgaagga   1080

gcattattaa gcaatagtat gttattgagt tgtttttttt ttattttaat gaatgacttg   1140

aatagtttgt ttatctgtac aaaatatcat aacaaaaatc agaagtcgaa actataatat   1200

ctaaactact cttccgttcc attttaaatg atgttttaag aaaataataa ttatgatata   1260

tcatatatta atggtattct ttcgttaaga gggaacataa gttataatac atatatcgtg   1320

tgatattgat gccttgatgg tattgtagag ttagatatat atatatatat atataatatt   1380

tccacagaat aatacacaaa caaaacaaac taattaaact cttttaaaaa ctaaaaagga   1440

gcataaatct attttcttat aattatgtga gaaacggata ttattattta tcacttattt   1500

ttttttggtg agaactatgc cttggctttg gctagtgctt taaatatggc ctttaatgag   1560

acaatctcat ttgtctatct ttttcactaa caaagaacaa aa                      1602


<210>  2
<211>  1572
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  promoter
<222>  (1)..(1572)
<223>  transcription regulating nucleotide sequence from Arabidopsis 
       thaliana gene At2g34700 (putative proline-rich glycoprotein)

<220>
<221>  TATA_signal
<222>  (1540)..(1547)
<223>  putative TATA-box

<400>  2
acattttacg gcactttctt gatcctcata ccatcatata tgtacacatg tatttagtga     60

atcaaaacta ttaaactatt ttaacatttt tcttctaaaa ttaataatta atttctaaat    120

ttccttaatt attgtacaaa aaattaaaag caataatttt ttaaaaattt taaatttcat    180

taattatatg atgaagttta taatttgttt tatttttttt ttttaattac aagatatata    240

tagtttcttt atgtttaact ttttttatat ttaatatttt cttaattcaa aacaaaaaaa    300

aatatatttt aatttttagt ttatttattt atttataaga tactaatagt ttctttatat    360

ttatcgtctt tacttttcaa tcctttttaa tttttatttt tcaaattata agagacataa    420

ctttttaata tttatcttct ttacttttta atcataaata ttttatgata aattaattaa    480

attatgtatt ggttgtttta tgaagtctag gtggcaagct attagatgct aacgtatcaa    540

gctgtgagaa agagttaagc tcctttcata tatataatac aactaaaaat ttgaatcgtc    600

aaagaaaaat acctttaaaa cagaaatttt ctgattttaa aagtgcaaaa aggttagtaa    660

tgccgaattt taacggaggc tattttgtcc aaaaaagatt gttaactagc gatgactatg    720

aggcttgaga ttcttcacca ataaatgccg actcgcttgg attagggata tatttcatca    780

agatacgtga ttactggtgc ccatgcattt acatagtaac cttacattgt taaatttaac    840

attttgtcac taggtaatct tacctaagaa aattaaaaat taaaaatatt atattttttt    900

gttgaataag taacacaact tgtcatgatg ggactatatc attaacacaa cttgaagcac    960

caaccacttt aattaattta tagcctcttc cctccatggc tctatcacca taagaaaact   1020

ttaaggtcat gtctttggct tgcacaatcc attgacaata gtattactat atatgaagga   1080

gcattattaa gcaatagtat gttattgagt tgtttttttt ttattttaat gaatgacttg   1140

aatagtttgt ttatctgtac aaaatatcat aacaaaaatc agaagtcgaa actataatat   1200

ctaaactact cttccgttcc attttaaatg atgttttaag aaaataataa ttatgatata   1260

tcatatatta atggtattct ttcgttaaga gggaacataa gttataatac atatatcgtg   1320

tgatattgat gccttgatgg tattgtagag ttagatatat atatatatat atataatatt   1380

tccacagaat aatacacaaa caaaacaaac taattaaact cttttaaaaa ctaaaaagga   1440

gcataaatct attttcttat aattatgtga gaaacggata ttattattta tcacttattt   1500

ttttttggtg agaactatgc cttggctttg gctagtgctt taaatatggc ctttaatgag   1560

acaatctcat tt                                                       1572


<210>  3
<211>  1601
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  promoter
<222>  (1)..(1601)
<223>  transcription regulating nucleotide sequence (derivative) from 
       Arabidopsis thaliana gene At2g34700 (putative proline-rich 
       glycoprotein) comprising 5-untranslated region

<220>
<221>  TATA_signal
<222>  (1539)..(1546)
<223>  putative TATA-box

<220>
<221>  5'UTR
<222>  (1572)..(1601)
<223>  putative 5-untranslated region

<400>  3
acattttacg gcactttctt gatcctcata ccatcatata tgtacacatg tatttagtga     60

atcaaaacta ttaaactatt ttaacatttt tcttctaaaa ttaataatta atttctaaat    120

ttccttaatt attgtacaaa aaattaaaag caataatttt ttaaaaattt taaatttcat    180

taattatatg atgaagttta taatttgttt tatttttttt tttaattaca agatatatat    240

agtttcttta tgtttaactt tttttatatt taatattttc ttaattcaaa acaaaaaaaa    300

atatatttta atttttagtt tatttattta tttataagat actaatagtt tctttatatt    360

tatcgtcttt acttttcaat cctttttaat ttttattttt caaattataa gagacataac    420

tttttaatat ttatcttctt tactttttaa tcataaatat tttatgataa attaattaaa    480

ttatgtattg gttgttttat gaagtctagg tggcaagcta ttagatgcta acgtatcaag    540

ctgtgagaaa gagttaagct cctttcatat atataataca actaaaaatt tgaatcgtca    600

aagaaaaata cctttaaaac agaaattttc tgattttaaa agtgcaaaaa ggttagtaat    660

gccgaatttt aacggaggct attttgtcca aaaaagattg ttaactagcg atgactatga    720

ggcttgagat tcttcaccaa taaatgccga ctcgcttgga ttagggatat atttcatcaa    780

gatacgtgat tactggtgcc catgcattta catagtaact ttacattgtt aaatttaaca    840

ttttgtcact aggtaatctt acctaagaaa attaaaaatt aaaaatatta tatttttttg    900

ttgaataagt aacacaactt gtcatgatgg gactatatca ttaacacaac ttgaagcacc    960

aactacttta attaatttat agcctcttcc ctccatggct ctatcaccat aagaaaactt   1020

taaggtcatg tctttggctt gcacaatcca ttgacaatag tattactata tatgaaggag   1080

cattattaag caatagtatg ttattgagtt gttttttttt tattttaatg aatgacttga   1140

atagtttgtt tatctgtaca aaatatcata acaaaaatca gaagtcgaaa ctataatatc   1200

taaactactc ttccgttcca ttttaaatga tgttttaaga aaataataat tatgatatat   1260

catatattaa tggtattctt tcgttaagag ggaacataag ttataataca tatatcatgt   1320

gatattgatg ccttgatggt attgtagagt tagatatata tatatatata tataatattt   1380

ccacagaata atacacaaac aaaacaaact aattaaactc ttttaaaaac taaaaaggag   1440

cataaatcta ttttcttata attatgtgag aaacggatat tattatttat cacttatttt   1500

tttttggtga gaactatgcc ttggctttgg ctagtgcttt aaatatggcc tttaatgaga   1560

caatctcatt tgtctatctt tttcactaac aaagaacaaa a                       1601


<210>  4
<211>  1571
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  promoter
<222>  (1)..(1571)
<223>  transcription regulating nucleotide sequence (derivative) from 
       Arabidopsis thaliana gene At2g34700 (putative proline-rich 
       glycoprotein)

<220>
<221>  TATA_signal
<222>  (1539)..(1546)
<223>  putative TATA-box

<400>  4
acattttacg gcactttctt gatcctcata ccatcatata tgtacacatg tatttagtga     60

atcaaaacta ttaaactatt ttaacatttt tcttctaaaa ttaataatta atttctaaat    120

ttccttaatt attgtacaaa aaattaaaag caataatttt ttaaaaattt taaatttcat    180

taattatatg atgaagttta taatttgttt tatttttttt tttaattaca agatatatat    240

agtttcttta tgtttaactt tttttatatt taatattttc ttaattcaaa acaaaaaaaa    300

atatatttta atttttagtt tatttattta tttataagat actaatagtt tctttatatt    360

tatcgtcttt acttttcaat cctttttaat ttttattttt caaattataa gagacataac    420

tttttaatat ttatcttctt tactttttaa tcataaatat tttatgataa attaattaaa    480

ttatgtattg gttgttttat gaagtctagg tggcaagcta ttagatgcta acgtatcaag    540

ctgtgagaaa gagttaagct cctttcatat atataataca actaaaaatt tgaatcgtca    600

aagaaaaata cctttaaaac agaaattttc tgattttaaa agtgcaaaaa ggttagtaat    660

gccgaatttt aacggaggct attttgtcca aaaaagattg ttaactagcg atgactatga    720

ggcttgagat tcttcaccaa taaatgccga ctcgcttgga ttagggatat atttcatcaa    780

gatacgtgat tactggtgcc catgcattta catagtaact ttacattgtt aaatttaaca    840

ttttgtcact aggtaatctt acctaagaaa attaaaaatt aaaaatatta tatttttttg    900

ttgaataagt aacacaactt gtcatgatgg gactatatca ttaacacaac ttgaagcacc    960

aactacttta attaatttat agcctcttcc ctccatggct ctatcaccat aagaaaactt   1020

taaggtcatg tctttggctt gcacaatcca ttgacaatag tattactata tatgaaggag   1080

cattattaag caatagtatg ttattgagtt gttttttttt tattttaatg aatgacttga   1140

atagtttgtt tatctgtaca aaatatcata acaaaaatca gaagtcgaaa ctataatatc   1200

taaactactc ttccgttcca ttttaaatga tgttttaaga aaataataat tatgatatat   1260

catatattaa tggtattctt tcgttaagag ggaacataag ttataataca tatatcatgt   1320

gatattgatg ccttgatggt attgtagagt tagatatata tatatatata tataatattt   1380

ccacagaata atacacaaac aaaacaaact aattaaactc ttttaaaaac taaaaaggag   1440

cataaatcta ttttcttata attatgtgag aaacggatat tattatttat cacttatttt   1500

tttttggtga gaactatgcc ttggctttgg ctagtgcttt aaatatggcc tttaatgaga   1560

caatctcatt t                                                        1571


<210>  5
<211>  733
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  CDS
<222>  (31)..(558)
<223>  coding for Arabidopsis thaliana At2g34700 gene product (putative 
       proline-rich glycoprotein)

<400>  5
gtccatcttt ttcactaaca aagaacaaaa atg ggt ctg gta aca aaa gct ctc      54
                                 Met Gly Leu Val Thr Lys Ala Leu        
                                 1               5                      

aaa tat ctc tta ctc ctt tct att tcc ttg act ttc atc tac acc aat      102
Lys Tyr Leu Leu Leu Leu Ser Ile Ser Leu Thr Phe Ile Tyr Thr Asn         
    10                  15                  20                          

gca gtt tca tcc gct tct cca atg act cca ccg tca tcg ccg gct aag      150
Ala Val Ser Ser Ala Ser Pro Met Thr Pro Pro Ser Ser Pro Ala Lys         
25                  30                  35                  40          

atg agc agg aga ctt gtg gca gtt gaa ggc atg gtt tat tgc aag tca      198
Met Ser Arg Arg Leu Val Ala Val Glu Gly Met Val Tyr Cys Lys Ser         
                45                  50                  55              

tgc aag tat tcc ggc gtc gat act ctc ttg gaa gca tct cct ctt caa      246
Cys Lys Tyr Ser Gly Val Asp Thr Leu Leu Glu Ala Ser Pro Leu Gln         
            60                  65                  70                  

gga gcg acc gta aag ctt gca tgc aac aac aca aag aga ggt gtg aca      294
Gly Ala Thr Val Lys Leu Ala Cys Asn Asn Thr Lys Arg Gly Val Thr         
        75                  80                  85                      

atg gag aca aaa aca gac aaa aac gga tac ttc ttc atg cta gcc cca      342
Met Glu Thr Lys Thr Asp Lys Asn Gly Tyr Phe Phe Met Leu Ala Pro         
    90                  95                  100                         

aaa aaa ctc acc acc tac gct ttc cac acg tgc cgg gct tgg cct acg      390
Lys Lys Leu Thr Thr Tyr Ala Phe His Thr Cys Arg Ala Trp Pro Thr         
105                 110                 115                 120         

aat cct gga ccc acg act gca acg atg aca tgc acc gtg ccg tca aag      438
Asn Pro Gly Pro Thr Thr Ala Thr Met Thr Cys Thr Val Pro Ser Lys         
                125                 130                 135             

ctc aac aat gga ata acc ggt gct atg ctt aag cct tct aag aca atc      486
Leu Asn Asn Gly Ile Thr Gly Ala Met Leu Lys Pro Ser Lys Thr Ile         
            140                 145                 150                 

aac atc ggt gaa cac gac tac gtt ctc ttc tct gtt ggt ccc ttc gcg      534
Asn Ile Gly Glu His Asp Tyr Val Leu Phe Ser Val Gly Pro Phe Ala         
        155                 160                 165                     

ttt gag cct gcc tgt gct ctt tga agactatact ttcgttttat gtatttggtg     588
Phe Glu Pro Ala Cys Ala Leu                                             
    170                 175                                             

tgtgcttgat tgttataatg atgatcacta gtgattttaa tgttctgtct tgtttgtttt    648

cttggtacgt ttccatttga aatttgtgtg ttttgacatt ataagatcgt tagtgtgcat    708

gtttgtagta aaaaaaaaaa aaaaa                                          733


<210>  6
<211>  175
<212>  PRT
<213>  Arabidopsis thaliana

<400>  6

Met Gly Leu Val Thr Lys Ala Leu Lys Tyr Leu Leu Leu Leu Ser Ile 
1               5                   10                  15      


Ser Leu Thr Phe Ile Tyr Thr Asn Ala Val Ser Ser Ala Ser Pro Met 
            20                  25                  30          


Thr Pro Pro Ser Ser Pro Ala Lys Met Ser Arg Arg Leu Val Ala Val 
        35                  40                  45              


Glu Gly Met Val Tyr Cys Lys Ser Cys Lys Tyr Ser Gly Val Asp Thr 
    50                  55                  60                  


Leu Leu Glu Ala Ser Pro Leu Gln Gly Ala Thr Val Lys Leu Ala Cys 
65                  70                  75                  80  


Asn Asn Thr Lys Arg Gly Val Thr Met Glu Thr Lys Thr Asp Lys Asn 
                85                  90                  95      


Gly Tyr Phe Phe Met Leu Ala Pro Lys Lys Leu Thr Thr Tyr Ala Phe 
            100                 105                 110         


His Thr Cys Arg Ala Trp Pro Thr Asn Pro Gly Pro Thr Thr Ala Thr 
        115                 120                 125             


Met Thr Cys Thr Val Pro Ser Lys Leu Asn Asn Gly Ile Thr Gly Ala 
    130                 135                 140                 


Met Leu Lys Pro Ser Lys Thr Ile Asn Ile Gly Glu His Asp Tyr Val 
145                 150                 155                 160 


Leu Phe Ser Val Gly Pro Phe Ala Phe Glu Pro Ala Cys Ala Leu 
                165                 170                 175 


<210>  7
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  7
ttttttgtcg acacatttta cggcactttc ttgatcctca tacca                     45


<210>  8
<211>  43
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  8
ttttttggat ccttttgttc tttgttagtg aaaaagatag aca                       43


<210>  9
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  9
ttttttggat ccaaatgaga ttgtctcatt aa                                   32


<210>  10
<211>  4
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (core region)

<400>  10
tcat                                                                   4


<210>  11
<211>  4
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (core region)

<400>  11
tcct                                                                   4


<210>  12
<211>  4
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (core region)

<400>  12
aaag                                                                   4


<210>  13
<211>  4
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (core region)


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<400>  13
acnt                                                                   4


<210>  14
<211>  4
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (core region)

<400>  14
catg                                                                   4


<210>  15
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (conserved region of motif)

<400>  15
tcatagtcat cgctagttaa caatc                                           25


<210>  16
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (conserved region of motif)

<400>  16
tcctttcata tatat                                                      15


<210>  17
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (conserved region of motif)

<400>  17
aggattgaaa agtaaa                                                     16


<210>  18
<211>  10
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (conserved region of motif)

<400>  18
tgacttgaat                                                            10


<210>  19
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (conserved region of motif)

<400>  19
catgca                                                                 6


<210>  20
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (motif matrix complete)

<400>  20
ctcatagtca tcgctagtta acaatct                                         27


<210>  21
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (motif matrix complete)

<400>  21
tcctttcata tatataa                                                    17


<210>  22
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (motif matrix complete)

<400>  22
aggattgaaa agtaaag                                                    17


<210>  23
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (motif matrix complete)

<400>  23
atgaatgact tgaatag                                                    17


<210>  24
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  promoter motif (motif matrix complete)

<400>  24
ctggtgccca tgcatttaca tagtaac                                         27


