                         SEQUENCE LISTING

<110>  The Queen's University of Belfast
       Todd, Daniel
       Ball, Neris
       Adair, Brian
       Wylie, Mildred
 
<120>  Chicken virus vaccine and diagnostic

<130>  P41580A

<150>  GB0600081.4
<151>  2006-01-04

<160>  9     

<170>  PatentIn version 3.3

<210>  1
<211>  391
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence obtained from chicken astrovirus type 3

<400>  1
aaagcccttg ttttggcgca ttaggcagat tcggtttttt tttttagcct caaagtataa     60

gacgcaggaa aacaaggagc tgtttgattg gtacaccaaa aaccttttgg agaaggtgat    120

attgttacct actggggaag tgtgccaaat aaagcgagga aatccttcag ggcaattttc    180

taccaccgtg gataacaaca tgtgcaacgt atggttaacc acctttgaaa tagcatggct    240

ccaccgcaaa caacggggca gactaccaac cccagctgaa ttgcgtgaaa acgtttgtta    300

tatttgctac ggtgatgaca ggctcttatc agtttcgaga gactttgtca tttatgagcc    360

tgaaactgtg gtagcaatgt acgcagatgt a                                   391


<210>  2
<211>  391
<212>  DNA
<213>  Artificial

<220>
<223>  sequence obtained from chicken astrovirus type 3

<400>  2
aaaacccttg ttttggcgca ttaggcagat tcggtttttc tttttagcct caaagtataa     60

gacgcaggaa aacaaggagc tgtttgattg gtacaccaaa aaccttttgg agaaggtgat    120

attgttacct actggggaag tgtgccaaat aaagcgagga aatccttcag ggcaactttc    180

taccaccgtg gataacaaca tgtgcaacgt atggttaacc acctttgaaa tagcatggct    240

ccaccgcaaa caacggggca gactaccaac cccagctgaa ttgcgtgaaa acgtttgtta    300

tatttgctac ggtgatgaca ggcccttatc agtttcgaga gactttgtca tttatgagcc    360

tgaaactgtg gtagcaatgt acgcagatgt a                                   391


<210>  3
<211>  391
<212>  DNA
<213>  Artificial

<220>
<223>  FP3 sequence

<400>  3
aaagcccttg ttttggcgta ttaggcagat tcggtttttc ttcttagcct caaagtataa     60

gacgcaggaa aacaaggacc tctttgattg gtacaccaaa aacctcttgg agaaggtgat    120

attgttacct actggagaag tgtgccaaat aaagcgaggg aatccttcag ggcaattttc    180

tactaccgtg gataacaaca tgtgcaatgt atggctaacc acctttgaaa tagcatggct    240

tcaccgcaaa caacggggta gattaccaac cccagctgaa ttgcgtgaaa atgtttgtta    300

tatttgctac ggtgatgata ggctcttatc agtttcaaga gactttgtca tttatgagcc    360

tgacactgtg gtagcgatgt acgctgatgt a                                   391


<210>  4
<211>  3265
<212>  DNA
<213>  Artificial

<220>
<223>  sequence obtained from chicken astrovirus type 3

<400>  4
aaagcccttg ttttggcgca ttaggcagat tcggtttttt tttttagcct caaagtataa     60

gacgcaggaa aacaaggagc tgtttgattg gtacaccaaa aaccttttgg agaaggtgat    120

attgttacct actggggaag tgtgccaaat aaagcgagga aatccttcag ggcaattttc    180

taccaccgtg gataacaaca tgtgcaacgt atggttaacc acctttgaaa tagcatggct    240

ccaccgcaaa caacggggca gactaccaac cccagctgaa ttgcgtgaaa acgtttgtta    300

tatttgctac ggtgatgaca ggctcttatc agtttcgaga gactttgtct tttatgagcc    360

tgaaactgtg gtagcaatgt acgcagatgt attcggcatg tgggtgaagc cagagaatgt    420

gaaggtaaga aatacactta gtggtctctc tttctgtggt atgacaatta caaaaaatca    480

gcatggccgt tatgttggta ttcctaatgt caataaaata ctgtccactc tacggtctcc    540

tacaaagcgc cttccaaatg tagaagcact atggggtaag ttgatatcat taagaattct    600

gtgtgagaat gcagatcccg acgtaaagga ttacttagat aggcagatca attgcgtcga    660

ggagtatgcc gctgctgaag aaatacagtt accagaagtc gggcccgact tctttcagaa    720

aatctggtag agggatggac cgaaatatag cagcatggcc gataaggcta gcgcgcagaa    780

ggagaaaaca acaaggcgcg gacgtggccg ttctcgatct aggtcacgtt ctcgttcccg    840

ttctaggaat cgtgtcaaga aaactgtcac gatagttgaa tctaagaaaa ccccatctag    900

atctatatta agaaaagaac ttgaaaatca tgagagaagg gataggaggc gttttaggaa    960

gattgaaaaa aaattaaatg gccctaaaat acatgatcgc atggcagtca caactacact   1020

tggagtcctc actggaaatt ctgacaataa tttggaaagg aaaatgagag ctcttcttaa   1080

cccattgctt ttgaaatctc aaaacactgg ggcctcagca tccccacttt cccttagggc   1140

atctcagtat tcaatgtgga agatacagaa atgtgttgta aaatttgttc ccctagttgg   1200

ggctgctaat gtggcaggta gtgtatcctt tgtgtctctg gatcaggatg caacctcctc   1260

ccagcctgaa tcacctgata cgataaaggc aaaggtgcat gcagaagttg caattggaca   1320

aagatttaat tggaacgttc aatctagata cctggtcgga ccccgttctg gttggtgggg   1380

catggatact ggtgagtcac caactgatac agttggacca gcccttgact tttggaatct   1440

ttatagaaca gtgaacacac ttcaaactgg ctcaacatcg caggcataca ctgcaccatt   1500

gttttctatt gaagtgtata cggtgtatgt tttttcaggt tacgaaccaa agcctgccct   1560

ggcgaccatg acaaattcaa cttttgagag tcagcagggg gtgaccataa caaatggtgc   1620

taatggtgaa cttctgctca atgttccacg gcgatcgagt cttgccgaag ggctgcgtga   1680

aaaggaagta ttataccgcg gccaaaacca aacgggtggt gtcggtgagg tactgtgggc   1740

ggtggcatca ggagctgttg aaggagctgc agaagcgttg ggcccatggg gatggttact   1800

gagaggtggc tggtgggtga taaagaagtt gtttggacgg agcgctgaaa atgaaagtga   1860

cgattatgtg atgtactcgt ctattgagga tgccaacaaa gatagtagga tctatcaaac   1920

ggtatccagt gcggtccctg ttcaacaagg tcctctggtt ctcacccaaa tctcatcccc   1980

aaatgttaat caagctgggg gtgttgtgca ggtaggtaca acaattgcca ctgattactt   2040

gccactatct caggcccagg ttccgctttt agagaacatt ctttactcca gcactgggca   2100

gcctgtgaca tcaactaaga gccatactat gaggatcact gggtttccag cctcgaaatt   2160

ggtaacatca acgtcgtcgc aatggttggg cactactgat acgagtgtcc aagcaacaaa   2220

gtggctaatg tcggattata cagacactgg agtgatattt ggctttccat actctgatga   2280

ttcccccggg gaaacttttg gtaacattgg agtaatacac acagccaagt cgctcataaa   2340

aacggtcaca tcaaggcgac aacgcgggct acgcatgtct ccacttgttt cgacattgtt   2400

accatcaact tctaagggac caacccagat gcttagttgc tttgacacgc cttactattg   2460

gattagggtt tgtgacaata cctgctcaaa caaacccaca aatggcgccg tgacacagcg   2520

ctgcaatgct tggggcgtta tggtggtgag cttagcacac aataaagtct acatcttggc   2580

tggttatccc gattctcaaa ctagggtacc acaacaacaa ctagtctggg acacttttga   2640

ctgggatgct acattttcta ctggcaggat ttataataca acatggccag gactttatga   2700

agaaagtgat gatgaaacag atgccgaatc tgacatctcc agtctttttg accccgttaa   2760

tgaggttgag caggactttc acttcaaatg tagtctgaag acatctgact acttgaagga   2820

ggaggctgat tattggaaag caaaagcaca acaattgctt atggagaaag caatgggaaa   2880

aaataatgac tctcctccac ttgtccgctt tgagaagggc ggacctgagc agcaaaaaca   2940

gcctgctagc agccgcggcc acgccgagta ggatcgaggg tacagctgca ccttcttcat   3000

ggagttttta tgccataatc aggcttttct ccatgtaaat caaggcaccg gggccacgcc   3060

gagcaggaac gagggtacag tgccgggttg accccacctg aaaggggcgt ccgccggtgt   3120

gataatcacc acaccggggc ctggtttaaa tcacagataa tcactctgtg tgtcaatcag   3180

gtctttcggg cggttttgga aacactagtt tttaaaacca atttgatttt gaattagatt   3240

aattggcaaa aaaaaaaaaa aaaaa                                         3265


<210>  5
<211>  738
<212>  PRT
<213>  Artificial

<220>
<223>  Protein encoded by chicken astrovirus type 3

<400>  5

Met Ala Asp Lys Ala Ser Ala Gln Lys Glu Lys Thr Thr Arg Arg Gly 
1               5                   10                  15      


Arg Gly Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Asn 
            20                  25                  30          


Arg Val Lys Lys Thr Val Thr Ile Val Glu Ser Lys Lys Thr Pro Ser 
        35                  40                  45              


Arg Ser Ile Leu Arg Lys Glu Leu Glu Asn His Glu Arg Arg Asp Arg 
    50                  55                  60                  


Arg Arg Phe Arg Lys Ile Glu Lys Lys Leu Asn Gly Pro Lys Ile His 
65                  70                  75                  80  


Asp Arg Met Ala Val Thr Thr Thr Leu Gly Val Leu Thr Gly Asn Ser 
                85                  90                  95      


Asp Asn Asn Leu Glu Arg Lys Met Arg Ala Leu Leu Asn Pro Leu Leu 
            100                 105                 110         


Leu Lys Ser Gln Asn Thr Gly Ala Ser Ala Ser Pro Leu Ser Leu Arg 
        115                 120                 125             


Ala Ser Gln Tyr Ser Met Trp Lys Ile Gln Lys Cys Val Val Lys Phe 
    130                 135                 140                 


Val Pro Leu Val Gly Ala Ala Asn Val Ala Gly Ser Val Ser Phe Val 
145                 150                 155                 160 


Ser Leu Asp Gln Asp Ala Thr Ser Ser Gln Pro Glu Ser Pro Asp Thr 
                165                 170                 175     


Ile Lys Ala Lys Val His Ala Glu Val Ala Ile Gly Gln Arg Phe Asn 
            180                 185                 190         


Trp Asn Val Gln Ser Arg Tyr Leu Val Gly Pro Arg Ser Gly Trp Trp 
        195                 200                 205             


Gly Met Asp Thr Gly Glu Ser Pro Thr Asp Thr Val Gly Pro Ala Leu 
    210                 215                 220                 


Asp Phe Trp Asn Leu Tyr Arg Thr Val Asn Thr Leu Gln Thr Gly Ser 
225                 230                 235                 240 


Thr Ser Gln Ala Tyr Thr Ala Pro Leu Phe Ser Ile Glu Val Tyr Thr 
                245                 250                 255     


Val Tyr Val Phe Ser Gly Tyr Glu Pro Lys Pro Ala Leu Ala Thr Met 
            260                 265                 270         


Thr Asn Ser Thr Phe Glu Ser Gln Gln Gly Val Thr Ile Thr Asn Gly 
        275                 280                 285             


Ala Asn Gly Glu Leu Leu Leu Asn Val Pro Arg Arg Ser Ser Leu Ala 
    290                 295                 300                 


Glu Gly Leu Arg Glu Lys Glu Val Leu Tyr Arg Gly Gln Asn Gln Thr 
305                 310                 315                 320 


Gly Gly Val Gly Glu Val Leu Trp Ala Val Ala Ser Gly Ala Val Glu 
                325                 330                 335     


Gly Ala Ala Glu Ala Leu Gly Pro Trp Gly Trp Leu Leu Arg Gly Gly 
            340                 345                 350         


Trp Trp Val Ile Lys Lys Leu Phe Gly Arg Ser Ala Glu Asn Glu Ser 
        355                 360                 365             


Asp Asp Tyr Val Met Tyr Ser Ser Ile Glu Asp Ala Asn Lys Asp Ser 
    370                 375                 380                 


Arg Ile Tyr Gln Thr Val Ser Ser Ala Val Pro Val Gln Gln Gly Pro 
385                 390                 395                 400 


Leu Val Leu Thr Gln Ile Ser Ser Pro Asn Val Asn Gln Ala Gly Gly 
                405                 410                 415     


Val Val Gln Val Gly Thr Thr Ile Ala Thr Asp Tyr Leu Pro Leu Ser 
            420                 425                 430         


Gln Ala Gln Val Pro Leu Leu Glu Asn Ile Leu Tyr Ser Ser Thr Gly 
        435                 440                 445             


Gln Pro Val Thr Ser Thr Lys Ser His Thr Met Arg Ile Thr Gly Phe 
    450                 455                 460                 


Pro Ala Ser Lys Leu Val Thr Ser Thr Ser Ser Gln Trp Leu Gly Thr 
465                 470                 475                 480 


Thr Asp Thr Ser Val Gln Ala Thr Lys Trp Leu Met Ser Asp Tyr Thr 
                485                 490                 495     


Asp Thr Gly Val Ile Phe Gly Phe Pro Tyr Ser Asp Asp Ser Pro Gly 
            500                 505                 510         


Glu Thr Phe Gly Asn Ile Gly Val Ile His Thr Ala Lys Ser Leu Ile 
        515                 520                 525             


Lys Thr Val Thr Ser Arg Arg Gln Arg Gly Leu Arg Met Ser Pro Leu 
    530                 535                 540                 


Val Ser Thr Leu Leu Pro Ser Thr Ser Lys Gly Pro Thr Gln Met Leu 
545                 550                 555                 560 


Ser Cys Phe Asp Thr Pro Tyr Tyr Trp Ile Arg Val Cys Asp Asn Thr 
                565                 570                 575     


Cys Ser Asn Lys Pro Thr Asn Gly Ala Val Thr Gln Arg Cys Asn Ala 
            580                 585                 590         


Trp Gly Val Met Val Val Ser Leu Ala His Asn Lys Val Tyr Ile Leu 
        595                 600                 605             


Ala Gly Tyr Pro Asp Ser Gln Thr Arg Val Pro Gln Gln Gln Leu Val 
    610                 615                 620                 


Trp Asp Thr Phe Asp Trp Asp Ala Thr Phe Ser Thr Gly Arg Ile Tyr 
625                 630                 635                 640 


Asn Thr Thr Trp Pro Gly Leu Tyr Glu Glu Ser Asp Asp Glu Thr Asp 
                645                 650                 655     


Ala Glu Ser Asp Ile Ser Ser Leu Phe Asp Pro Val Asn Glu Val Glu 
            660                 665                 670         


Gln Asp Phe His Phe Lys Cys Ser Leu Lys Thr Ser Asp Tyr Leu Lys 
        675                 680                 685             


Glu Glu Ala Asp Tyr Trp Lys Ala Lys Ala Gln Gln Leu Leu Met Glu 
    690                 695                 700                 


Lys Ala Met Gly Lys Asn Asn Asp Ser Pro Pro Leu Val Arg Phe Glu 
705                 710                 715                 720 


Lys Gly Gly Pro Glu Gln Gln Lys Gln Pro Ala Ser Ser Arg Gly His 
                725                 730                 735     


Ala Glu 
        


<210>  6
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer

<400>  6
agcctcaaag tataagacgc ag                                              22


<210>  7
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer

<400>  7
ccatgctatt tcaaaggtgg tt                                              22


<210>  8
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  AstPol-1F


<220>
<221>  modified_base
<222>  (9)..(9)
<223>  I

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  modified base

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  modified base

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  I

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  modified base

<220>
<221>  modified_base
<222>  (21)..(21)
<223>  I

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  modified base

<220>
<221>  modified_base
<222>  (24)..(24)
<223>  I

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  modified base

<220>
<221>  modified_base
<222>  (27)..(27)
<223>  I

<400>  8
gaytggacnm gntaygaygg nacnatncc                                       29


<210>  9
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  AstPol-1R


<220>
<221>  modified_base
<222>  (4)..(4)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  modified base

<220>
<221>  modified_base
<222>  (13)..(13)

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  modified base

<400>  9
yttnacccac atnccraa                                                   18


