                         SEQUENCE LISTING

<110>  VIB vzw
       Katholieke Universiteit Leuven, K.U.Leuven R&D
 
<120>  NOVEL BIFUNCTIONAL TREHALOSE SYNTHASE

<130>  JTH/Ots/V240

<150>  EP 06116357.2
<151>  2006-06-29

<160>  4     

<170>  PatentIn version 3.3

<210>  1
<211>  2202
<212>  DNA
<213>  Cytophaga hutchinsonii

<400>  1
atgaatgacg aaaaaagatt aattattgta gcatatagaa taccttttaa agttacgttt     60

aaagaaagcg gagagcgcgt attacaacaa aattcaggag ggcttgtttc cgctatccta    120

tcattggcag aaaaaggaga tgtgtttggt aaaaatcaaa aaattcattg gtttggttat    180

tccacaccag atattttaca ggagggagaa acgcaggttg aaaatgaaaa ttttattgta    240

catcctgtct taattgaaga tgcactaaat gatgcgtatt acgaaggatt ttgtaacagt    300

acgatttggc ccatttcgca ttattatccc tatctcacca gctttaatga agaggattat    360

gaagcgtatg taaaagcaaa taaaatattt gcagatcaga ttaatgcctt tatccgtccg    420

ggagatatgg tctggataca ggattatcag ctgatgttat tgcctggcat gctccgttca    480

gaaaatccgg atgcttctat cggttatttc catcacatcc cttttgcaac gtttgaaatt    540

tttcgtttgc ttccgaaaca atggcaaaca gaattgttaa aaggtatgat cggcgcagat    600

ctggttggtt ttcataccaa tgattacagc cagtattttt tgagaagtgt gcagcgtgta    660

ttaggctatg atactactgc ccgcgacata agtattccaa acaggatcgt gcgggtagac    720

acatttccca tcagcattga ttataataaa tttcaggaat tgtataatga tccgggcgta    780

attgaaaaaa ggaaagagtt tttaaaagac ctcagtgcag agaaaaatat tttttccgtt    840

gaccggctag attattcaaa aggattgatc catcggttaa atgggtttga atattttctg    900

gatcagtatc cgcaatggca tacacgtgtt gtacttacaa tgattgtagt tccttcgcgt    960

gaaacaatcc tgaagtatgc tgagatgaaa cacgaactgg aagaagcggt aggccgcatt   1020

aacggtaagt acggtacctt aggctggcgt ccggttatat atctgtaccg ttcgttaagc   1080

tttgaagaat taggcgcgtt atatacactg gccgatgtgg cactcatcac gcccatccgt   1140

gacgggatga atttagttgc aaaagaattt gttgctacgc gtaccgataa aaaaggcgta   1200

ttgattttaa gtgaaatggc aggtgcgtcg ctggaattga atggtgcaat actgatcaac   1260

ccaacagaca agaggacact ggccgcagcc attattcagg cattaaccat ggacgagcag   1320

gagcagacac aacgtatgca gatgatgcag gaccggatcc gtgtgtatga tatatttaaa   1380

tggacagaag actacatgga tcaattagat attataaaat tgaaacaaat ggaattcgaa   1440

gagaaaatta taaaccagga tgtgttaaaa agtttcaaac agatttttga tgaatcaagc   1500

cggcgcttgt ttttattgga ttatgacgga accttatctc ccattgttcc tgacccctcc   1560

agttctaagc caagtgtagg tattgttgat gtattaaaaa aactcagcag tgattttaat   1620

gtaaatgttg ctatcataag cggaagagat aaagagtttt tgaattatat ctttgatcca   1680

aatacaattc tgtctgcaga gcatggcgcg ttcttacgta ttccgggtca ggattgggag   1740

ctgcaatatg attacaatga tcagtggaag cgttctgtat tgccggtgtt tcagaagtat   1800

accgatcgtt gcgccggatc ttttattgaa aataaatcaa catcacttgc ctggcattac   1860

agaaatacag aaaaagaata tgctcatatc cgttccagag aatttattga agaactggaa   1920

aacaaagttg ggataaaatc aaatctcgcc attattgatg gtgataaagt aattgaaatg   1980

aaaccttctg atgtagataa ggggattgct gcgcggaaaa tatgtgatct gtatcagccg   2040

gattttattt tatctattgg agatgaccgg actgatgaag acatgtttaa agcgcttccg   2100

gataatgcgc ttacaattaa agttggcgtt aaaaatagtt ctgccaagtt tacaattaaa   2160

acgcaggaag aagtaatgac cattctgaat acgtttttat aa                      2202


<210>  2
<211>  733
<212>  PRT
<213>  Cytophaga hutchinsonii

<400>  2

Met Asn Asp Glu Lys Arg Leu Ile Ile Val Ala Tyr Arg Ile Pro Phe 
1               5                   10                  15      


Lys Val Thr Phe Lys Glu Ser Gly Glu Arg Val Leu Gln Gln Asn Ser 
            20                  25                  30          


Gly Gly Leu Val Ser Ala Ile Leu Ser Leu Ala Glu Lys Gly Asp Val 
        35                  40                  45              


Phe Gly Lys Asn Gln Lys Ile His Trp Phe Gly Tyr Ser Thr Pro Asp 
    50                  55                  60                  


Ile Leu Gln Glu Gly Glu Thr Gln Val Glu Asn Glu Asn Phe Ile Val 
65                  70                  75                  80  


His Pro Val Leu Ile Glu Asp Ala Leu Asn Asp Ala Tyr Tyr Glu Gly 
                85                  90                  95      


Phe Cys Asn Ser Thr Ile Trp Pro Ile Ser His Tyr Tyr Pro Tyr Leu 
            100                 105                 110         


Thr Ser Phe Asn Glu Glu Asp Tyr Glu Ala Tyr Val Lys Ala Asn Lys 
        115                 120                 125             


Ile Phe Ala Asp Gln Ile Asn Ala Phe Ile Arg Pro Gly Asp Met Val 
    130                 135                 140                 


Trp Ile Gln Asp Tyr Gln Leu Met Leu Leu Pro Gly Met Leu Arg Ser 
145                 150                 155                 160 


Glu Asn Pro Asp Ala Ser Ile Gly Tyr Phe His His Ile Pro Phe Ala 
                165                 170                 175     


Thr Phe Glu Ile Phe Arg Leu Leu Pro Lys Gln Trp Gln Thr Glu Leu 
            180                 185                 190         


Leu Lys Gly Met Ile Gly Ala Asp Leu Val Gly Phe His Thr Asn Asp 
        195                 200                 205             


Tyr Ser Gln Tyr Phe Leu Arg Ser Val Gln Arg Val Leu Gly Tyr Asp 
    210                 215                 220                 


Thr Thr Ala Arg Asp Ile Ser Ile Pro Asn Arg Ile Val Arg Val Asp 
225                 230                 235                 240 


Thr Phe Pro Ile Ser Ile Asp Tyr Asn Lys Phe Gln Glu Leu Tyr Asn 
                245                 250                 255     


Asp Pro Gly Val Ile Glu Lys Arg Lys Glu Phe Leu Lys Asp Leu Ser 
            260                 265                 270         


Ala Glu Lys Asn Ile Phe Ser Val Asp Arg Leu Asp Tyr Ser Lys Gly 
        275                 280                 285             


Leu Ile His Arg Leu Asn Gly Phe Glu Tyr Phe Leu Asp Gln Tyr Pro 
    290                 295                 300                 


Gln Trp His Thr Arg Val Val Leu Thr Met Ile Val Val Pro Ser Arg 
305                 310                 315                 320 


Glu Thr Ile Leu Lys Tyr Ala Glu Met Lys His Glu Leu Glu Glu Ala 
                325                 330                 335     


Val Gly Arg Ile Asn Gly Lys Tyr Gly Thr Leu Gly Trp Arg Pro Val 
            340                 345                 350         


Ile Tyr Leu Tyr Arg Ser Leu Ser Phe Glu Glu Leu Gly Ala Leu Tyr 
        355                 360                 365             


Thr Leu Ala Asp Val Ala Leu Ile Thr Pro Ile Arg Asp Gly Met Asn 
    370                 375                 380                 


Leu Val Ala Lys Glu Phe Val Ala Thr Arg Thr Asp Lys Lys Gly Val 
385                 390                 395                 400 


Leu Ile Leu Ser Glu Met Ala Gly Ala Ser Leu Glu Leu Asn Gly Ala 
                405                 410                 415     


Ile Leu Ile Asn Pro Thr Asp Lys Arg Thr Leu Ala Ala Ala Ile Ile 
            420                 425                 430         


Gln Ala Leu Thr Met Asp Glu Gln Glu Gln Thr Gln Arg Met Gln Met 
        435                 440                 445             


Met Gln Asp Arg Ile Arg Val Tyr Asp Ile Phe Lys Trp Thr Glu Asp 
    450                 455                 460                 


Tyr Met Asp Gln Leu Asp Ile Ile Lys Leu Lys Gln Met Glu Phe Glu 
465                 470                 475                 480 


Glu Lys Ile Ile Asn Gln Asp Val Leu Lys Ser Phe Lys Gln Ile Phe 
                485                 490                 495     


Asp Glu Ser Ser Arg Arg Leu Phe Leu Leu Asp Tyr Asp Gly Thr Leu 
            500                 505                 510         


Ser Pro Ile Val Pro Asp Pro Ser Ser Ser Lys Pro Ser Val Gly Ile 
        515                 520                 525             


Val Asp Val Leu Lys Lys Leu Ser Ser Asp Phe Asn Val Asn Val Ala 
    530                 535                 540                 


Ile Ile Ser Gly Arg Asp Lys Glu Phe Leu Asn Tyr Ile Phe Asp Pro 
545                 550                 555                 560 


Asn Thr Ile Leu Ser Ala Glu His Gly Ala Phe Leu Arg Ile Pro Gly 
                565                 570                 575     


Gln Asp Trp Glu Leu Gln Tyr Asp Tyr Asn Asp Gln Trp Lys Arg Ser 
            580                 585                 590         


Val Leu Pro Val Phe Gln Lys Tyr Thr Asp Arg Cys Ala Gly Ser Phe 
        595                 600                 605             


Ile Glu Asn Lys Ser Thr Ser Leu Ala Trp His Tyr Arg Asn Thr Glu 
    610                 615                 620                 


Lys Glu Tyr Ala His Ile Arg Ser Arg Glu Phe Ile Glu Glu Leu Glu 
625                 630                 635                 640 


Asn Lys Val Gly Ile Lys Ser Asn Leu Ala Ile Ile Asp Gly Asp Lys 
                645                 650                 655     


Val Ile Glu Met Lys Pro Ser Asp Val Asp Lys Gly Ile Ala Ala Arg 
            660                 665                 670         


Lys Ile Cys Asp Leu Tyr Gln Pro Asp Phe Ile Leu Ser Ile Gly Asp 
        675                 680                 685             


Asp Arg Thr Asp Glu Asp Met Phe Lys Ala Leu Pro Asp Asn Ala Leu 
    690                 695                 700                 


Thr Ile Lys Val Gly Val Lys Asn Ser Ser Ala Lys Phe Thr Ile Lys 
705                 710                 715                 720 


Thr Gln Glu Glu Val Met Thr Ile Leu Asn Thr Phe Leu 
                725                 730             


<210>  3
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer

<400>  3
ggcctcgaga tgaatgacga aaaagattaa tt                                   32


<210>  4
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer

<400>  4
ggcgagctca taaaacgtat tcagaatgg                                       29


