                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  A NOVEL WILD-TYPE LIPASE, ITS POLYPEPTIDES AND POLYNUCLEOTIDES 
       ENCODING THE POLYPEPTIDES

<130>  

<160>  17    

<170>  PatentIn version 3.3

<210>  1
<211>  1219
<212>  DNA
<213>  Nectria sp. 


<220>
<221>  CDS
<222>  (41)..(1069)

<220>
<221>  sig_peptide
<222>  (41)..(124)

<220>
<221>  mat_peptide
<222>  (125)..(1069)

<400>  1
acaggacccc tagacttgtg taactctcct gattctcaca atg cgt ctt ctc cct       55
                                            Met Arg Leu Leu Pro         
                                                        -25             

gcc ctc tcc gtg gtc ggc gtt gcc agc gct gcc tcc atc aag agc tat      103
Ala Leu Ser Val Val Gly Val Ala Ser Ala Ala Ser Ile Lys Ser Tyr         
            -20                 -15                 -10                 

ctt cat gcc ttt gag gag cga gct gtt act gtg acc tcc cag aac ctc      151
Leu His Ala Phe Glu Glu Arg Ala Val Thr Val Thr Ser Gln Asn Leu         
        -5              -1  1               5                           

gca aac ttc aag ttc tac gtc cag cat gcc act gcc gcg tac tgt aac      199
Ala Asn Phe Lys Phe Tyr Val Gln His Ala Thr Ala Ala Tyr Cys Asn         
10                  15                  20                  25          

tac gac cgc gca gct gga gcc ttg att tca tgc tcg agc aac tgc cca      247
Tyr Asp Arg Ala Ala Gly Ala Leu Ile Ser Cys Ser Ser Asn Cys Pro         
                30                  35                  40              

agt att gaa agc aat gct gct aag att gtg gga tcc ttc gga ggc gag      295
Ser Ile Glu Ser Asn Ala Ala Lys Ile Val Gly Ser Phe Gly Gly Glu         
            45                  50                  55                  

gat acg ggc att gca ggc tac gtc tca act gac gca act cgc aag gag      343
Asp Thr Gly Ile Ala Gly Tyr Val Ser Thr Asp Ala Thr Arg Lys Glu         
        60                  65                  70                      

att gtc gtc tct atc cgt ggc agt att aac gtc cgc aac tgg atc aca      391
Ile Val Val Ser Ile Arg Gly Ser Ile Asn Val Arg Asn Trp Ile Thr         
    75                  80                  85                          

aac ctc gac ttc gtc tgg agt tcc tgc tca gat ctg tcg agc aac tgc      439
Asn Leu Asp Phe Val Trp Ser Ser Cys Ser Asp Leu Ser Ser Asn Cys         
90                  95                  100                 105         

aag gcc cac gct ggc ttc aaa gat gct tgg gat gag atc tcc acc gct      487
Lys Ala His Ala Gly Phe Lys Asp Ala Trp Asp Glu Ile Ser Thr Ala         
                110                 115                 120             

gcc aaa gct gca gtc gtc tcg gcg aag aag gcc aac cca agc tac acc      535
Ala Lys Ala Ala Val Val Ser Ala Lys Lys Ala Asn Pro Ser Tyr Thr         
            125                 130                 135                 

atc gtc gcc acg gga cac tcc ctt ggt ggt gct gtt gct acc tta gca      583
Ile Val Ala Thr Gly His Ser Leu Gly Gly Ala Val Ala Thr Leu Ala         
        140                 145                 150                     

gct gct tac atc cga gct gct gga tat agt gtc gat ctg tac acg ttc      631
Ala Ala Tyr Ile Arg Ala Ala Gly Tyr Ser Val Asp Leu Tyr Thr Phe         
    155                 160                 165                         

ggc tcg cca cgt gta gga aat gac tac ttc gcc aac ttc gtc acc agc      679
Gly Ser Pro Arg Val Gly Asn Asp Tyr Phe Ala Asn Phe Val Thr Ser         
170                 175                 180                 185         

caa gcc gga gct gaa tac cgc gtg aca cac ctc gac gac cct gtt cct      727
Gln Ala Gly Ala Glu Tyr Arg Val Thr His Leu Asp Asp Pro Val Pro         
                190                 195                 200             

cgt ctt cca ccc atc ctc ttt ggc tac cgt cat acg tct cct gag tac      775
Arg Leu Pro Pro Ile Leu Phe Gly Tyr Arg His Thr Ser Pro Glu Tyr         
            205                 210                 215                 

tgg ctg tca aac gga ggc gct act acg acg acc tat agt ctg tca gac      823
Trp Leu Ser Asn Gly Gly Ala Thr Thr Thr Thr Tyr Ser Leu Ser Asp         
        220                 225                 230                     

atc gtg gta tgc gag ggt atc gcc aac acc gac tgc aat gcc ggc acg      871
Ile Val Val Cys Glu Gly Ile Ala Asn Thr Asp Cys Asn Ala Gly Thr         
    235                 240                 245                         

ctt ggc ctt gat att att gcc cac ctc ata tac ttc cag gat act tcg      919
Leu Gly Leu Asp Ile Ile Ala His Leu Ile Tyr Phe Gln Asp Thr Ser         
250                 255                 260                 265         

gca tgc aac acc gga ttc acg tgg aag cgc gac acg ttg tcg gat gca      967
Ala Cys Asn Thr Gly Phe Thr Trp Lys Arg Asp Thr Leu Ser Asp Ala         
                270                 275                 280             

gag ctc gag gag atg gtg aac aag tgg gct gag cag gat gtc gaa tac     1015
Glu Leu Glu Glu Met Val Asn Lys Trp Ala Glu Gln Asp Val Glu Tyr         
            285                 290                 295                 

gtc gcc aat ttg acg acg acc gcg tcg aag cga tgg aaa gga gca gtg     1063
Val Ala Asn Leu Thr Thr Thr Ala Ser Lys Arg Trp Lys Gly Ala Val         
        300                 305                 310                     

gct aac tagccatgat atcttgggaa gtttacttct tggaaagcag tggatgaaag      1119
Ala Asn                                                                 
    315                                                                 

taccttatga tcacattgaa gcaatgaact tacaatgata acgtacataa ataaattgtc   1179

tcgagagagt caaacgtcta aatgaaaaaa aaaaaaaaaa                         1219


<210>  2
<211>  343
<212>  PRT
<213>  Nectria sp. 

<400>  2

Met Arg Leu Leu Pro Ala Leu Ser Val Val Gly Val Ala Ser Ala Ala 
            -25                 -20                 -15         


Ser Ile Lys Ser Tyr Leu His Ala Phe Glu Glu Arg Ala Val Thr Val 
        -10                 -5              -1  1               


Thr Ser Gln Asn Leu Ala Asn Phe Lys Phe Tyr Val Gln His Ala Thr 
5                   10                  15                  20  


Ala Ala Tyr Cys Asn Tyr Asp Arg Ala Ala Gly Ala Leu Ile Ser Cys 
                25                  30                  35      


Ser Ser Asn Cys Pro Ser Ile Glu Ser Asn Ala Ala Lys Ile Val Gly 
            40                  45                  50          


Ser Phe Gly Gly Glu Asp Thr Gly Ile Ala Gly Tyr Val Ser Thr Asp 
        55                  60                  65              


Ala Thr Arg Lys Glu Ile Val Val Ser Ile Arg Gly Ser Ile Asn Val 
    70                  75                  80                  


Arg Asn Trp Ile Thr Asn Leu Asp Phe Val Trp Ser Ser Cys Ser Asp 
85                  90                  95                  100 


Leu Ser Ser Asn Cys Lys Ala His Ala Gly Phe Lys Asp Ala Trp Asp 
                105                 110                 115     


Glu Ile Ser Thr Ala Ala Lys Ala Ala Val Val Ser Ala Lys Lys Ala 
            120                 125                 130         


Asn Pro Ser Tyr Thr Ile Val Ala Thr Gly His Ser Leu Gly Gly Ala 
        135                 140                 145             


Val Ala Thr Leu Ala Ala Ala Tyr Ile Arg Ala Ala Gly Tyr Ser Val 
    150                 155                 160                 


Asp Leu Tyr Thr Phe Gly Ser Pro Arg Val Gly Asn Asp Tyr Phe Ala 
165                 170                 175                 180 


Asn Phe Val Thr Ser Gln Ala Gly Ala Glu Tyr Arg Val Thr His Leu 
                185                 190                 195     


Asp Asp Pro Val Pro Arg Leu Pro Pro Ile Leu Phe Gly Tyr Arg His 
            200                 205                 210         


Thr Ser Pro Glu Tyr Trp Leu Ser Asn Gly Gly Ala Thr Thr Thr Thr 
        215                 220                 225             


Tyr Ser Leu Ser Asp Ile Val Val Cys Glu Gly Ile Ala Asn Thr Asp 
    230                 235                 240                 


Cys Asn Ala Gly Thr Leu Gly Leu Asp Ile Ile Ala His Leu Ile Tyr 
245                 250                 255                 260 


Phe Gln Asp Thr Ser Ala Cys Asn Thr Gly Phe Thr Trp Lys Arg Asp 
                265                 270                 275     


Thr Leu Ser Asp Ala Glu Leu Glu Glu Met Val Asn Lys Trp Ala Glu 
            280                 285                 290         


Gln Asp Val Glu Tyr Val Ala Asn Leu Thr Thr Thr Ala Ser Lys Arg 
        295                 300                 305             


Trp Lys Gly Ala Val Ala Asn 
    310                 315 


<210>  3
<211>  24
<212>  DNA
<213>  primer-as1

<400>  3
tccagacgaa gtcgaggttt gtga                                            24


<210>  4
<211>  24
<212>  DNA
<213>  primer-as2

<400>  4
tactgccacg gatagagacg acaa                                            24


<210>  5
<211>  24
<212>  DNA
<213>  primer-as3

<400>  5
aatgcccgta tcctcgcctc cgaa                                            24


<210>  6
<211>  22
<212>  DNA
<213>  primer-s00

<400>  6
aacaggaccc ctagacttgt gt                                              22


<210>  7
<211>  20
<212>  DNA
<213>  primer-s01

<400>  7
taactctcct gattctcaca                                                 20


<210>  8
<211>  17
<212>  DNA
<213>  primer-n1


<220>
<221>  misc_feature
<223>  n is a, c, g or t

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<400>  8
cadaayytng cnaaytt                                                    17


<210>  9
<211>  20
<212>  DNA
<213>  primer-n2


<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<400>  9
aayttytayg tncadcaygc                                                 20


<210>  10
<211>  20
<212>  DNA
<213>  primer-FDR1


<220>
<221>  misc_feature
<223>  n is a, c, g or t

<400>  10
aggttggcdg cdccctcaca                                                 20


<210>  11
<211>  20
<212>  DNA
<213>  primer-FDR2

<400>  11
gwwgtgtgcc dgtatccaaa                                                 20


<210>  12
<211>  274
<212>  PRT
<213>  Thermomyces lanuginosus

<400>  12

Ser Pro Ile Arg Arg Glu Val Ser Gln Asp Leu Phe Asn Gln Phe Asn 
1               5                   10                  15      


Leu Phe Ala Gln Tyr Ser Ala Ala Ala Tyr Cys Gly Lys Asn Asn Asp 
            20                  25                  30          


Ala Pro Ala Gly Thr Asn Ile Thr Cys Thr Gly Asn Ala Cys Pro Glu 
        35                  40                  45              


Val Glu Lys Ala Asp Ala Thr Phe Leu Tyr Ser Phe Glu Asp Ser Gly 
    50                  55                  60                  


Val Gly Asp Val Thr Gly Phe Leu Ala Leu Asp Asn Thr Asn Lys Leu 
65                  70                  75                  80  


Ile Val Leu Ser Phe Arg Gly Ser Arg Ser Ile Glu Asn Trp Ile Gly 
                85                  90                  95      


Asn Leu Asn Phe Asp Leu Lys Glu Ile Asn Asp Ile Cys Ser Gly Cys 
            100                 105                 110         


Arg Gly His Asp Gly Phe Thr Ser Ser Trp Arg Ser Val Ala Asp Thr 
        115                 120                 125             


Leu Arg Gln Lys Val Glu Asp Ala Val Arg Glu His Pro Asp Tyr Arg 
    130                 135                 140                 


Val Val Phe Thr Gly His Ser Leu Gly Gly Ala Leu Ala Thr Val Ala 
145                 150                 155                 160 


Gly Ala Asp Leu Arg Gly Asn Gly Tyr Asp Ile Asp Val Phe Ser Tyr 
                165                 170                 175     


Gly Ala Pro Arg Val Gly Asn Arg Ala Phe Ala Glu Phe Leu Thr Val 
            180                 185                 190         


Gln Thr Gly Gly Thr Leu Tyr Arg Ile Thr His Thr Asn Asp Ile Val 
        195                 200                 205             


Pro Arg Leu Pro Pro Arg Glu Phe Gly Tyr Ser His Ser Ser Pro Glu 
    210                 215                 220                 


Tyr Trp Ile Lys Ser Gly Thr Leu Val Pro Val Arg Arg Arg Asp Ile 
225                 230                 235                 240 


Val Lys Ile Glu Gly Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn 
                245                 250                 255     


Ile Pro Asp Ile Pro Ala His Leu Trp Tyr Phe Gly Leu Ile Gly Thr 
            260                 265                 270         


Cys Leu 
        


<210>  13
<211>  12
<212>  PRT
<213>  synthetic sequence

<400>  13

Ala Cys Met Ser His Thr Trp Gly Glu Arg Asn Leu 
1               5                   10          


<210>  14
<211>  14
<212>  PRT
<213>  synthetic sequence

<400>  14

His Gly Trp Gly Glu Asp Ala Asn Leu Ala Met Asn Pro Ser 
1               5                   10                  


<210>  15
<211>  20
<212>  PRT
<213>  Nectria sp.

<400>  15

Ala Val Thr Val Thr Ser Gln Asn Leu Ala Asn Phe Lys Phe Tyr Val 
1               5                   10                  15      


Gln His Ala Thr 
            20  


<210>  16
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for PCR


<220>
<221>  misc_feature
<222>  (1)..(38)

<400>  16
gccggcggcc gcacaatgcg tcttctccct gccctctc                             38


<210>  17
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for pcr


<220>
<221>  misc_feature
<222>  (1)..(34)

<400>  17
tcgcggcgcg ccctagttag ccactgctcc tttc                                 34


