
                                SEQUENCE LISTING

<110> CENTOCOR, INC.
      
<120> BMP-7 VARIANT COMPOSITIONS, METHODS AND USES

<130> CEN5145PCT

<140> TO BE ASSIGNED      
<141> 2007-06-07  

<150> 60/820538
<151> 2006-07-27

<160> 48

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1293
<212> DNA
<213> Homo sapiens

<400> 1
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagcgcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctgc ggatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 2
<211> 431
<212> PRT
<213> Homo sapiens

<400> 2
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              

Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Arg Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Arg Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 3
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S and R314S mutations.

<400> 3
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 4
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S and R314S mutations and
      encoded by a Homo sapiens derived cDNA.

<400> 4
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             

Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 5
<211> 1337
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the ?S293-R299 and R314S mutations.

<400> 5
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttatgca cgtgcgctca ctgcgagctg cggcgccgca cagcttcgtg gcgctctggg 120
cacccctgtt cctgctgcgc tccgccctgg ccgacttcag cctggacaac gaggtgcact 180
cgagcttcat ccaccggcgc ctccgcagcc aggagcggcg ggagatgcag cgcgagatcc 240
tctccatttt gggcttgccc caccgcccgc gcccgcacct ccagggcaag cacaactcgg 300
cacccatgtt catgctggac ctgtacaacg ccatggcggt ggaggagggc ggcgggcccg 360
gcggccaggg cttctcctac ccctacaagg ccgtcttcag tacccagggc ccccctctgg 420
ccagcctgca agatagccat ttcctcaccg acgccgacat ggtcatgagc ttcgtcaacc 480
tcgtggaaca tgacaaggaa ttcttccacc cacgctacca ccatcgagag ttccggtttg 540
atctttccaa gatcccagaa ggggaagctg tcacggcagc cgaattccgg atctacaagg 600
actacatccg ggaacgcttc gacaatgaga cgttccggat cagcgtttat caggtgctcc 660
aggagcactt gggcagggaa tcggatctct tcctgctcga cagccgtacc ctctgggcct 720
cggaggaggg ctggctggtg tttgacatca cagccaccag caaccactgg gtggtcaatc 780
cgcggcacaa cctgggcctg cagctctcgg tggagacgct ggatgggcag agcatcaacc 840
ccaagttggc gggcctgatt gggcggcacg ggccccagaa caagcagccc ttcatggtgg 900
ctttcttcaa ggccacggag gtccacttcc gcagcatccg gagccagaac cgctccaaga 960
cgcccaagaa ccaggaagcc ctgagcatgg ccaacgtggc agagaacagc agcagcgacc 1020
agaggcaggc ctgtaagaag cacgagctgt atgtcagctt ccgagacctg ggctggcagg 1080
actggatcat cgcgcctgaa ggctacgccg cctactactg tgagggggag tgtgccttcc 1140
ctctgaactc ctacatgaac gccaccaacc acgccatcgt gcagacgctg gtccacttca 1200
tcaacccgga aacggtgccc aagccctgct gtgcgcccac gcagctcaat gccatctccg 1260
tcctctactt cgatgacagc tccaacgtca tcctgaagaa atacagaaac atggtggtcc 1320
gggcctgtgg ctgccac                                                1337

<210> 6
<211> 424
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the ?S293-R299 and R314S mutations and
      encoded by a Homo sapiens derived cDNA.

<400> 6
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Gln Asn Arg Ser Lys Thr Pro Lys Asn Gln Glu
    290                 295                 300                 
Ala Leu Ser Met Ala Asn Val Ala Glu Asn Ser Ser Ser Asp Gln Arg
305                 310                 315                 320 
Gln Ala Cys Lys Lys His Glu Leu Tyr Val Ser Phe Arg Asp Leu Gly
                325                 330                 335     
Trp Gln Asp Trp Ile Ile Ala Pro Glu Gly Tyr Ala Ala Tyr Tyr Cys
            340                 345                 350         
Glu Gly Glu Cys Ala Phe Pro Leu Asn Ser Tyr Met Asn Ala Thr Asn
        355                 360                 365             
His Ala Ile Val Gln Thr Leu Val His Phe Ile Asn Pro Glu Thr Val
    370                 375                 380                 
Pro Lys Pro Cys Cys Ala Pro Thr Gln Leu Asn Ala Ile Ser Val Leu
385                 390                 395                 400 
Tyr Phe Asp Asp Ser Ser Asn Val Ile Leu Lys Lys Tyr Arg Asn Met
                405                 410                 415     
Val Val Arg Ala Cys Gly Cys His
            420                 


<210> 7
<211> 1227
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the ?S293-M314 mutation.

<400> 7
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggatgg ccaacgtggc agagaacagc 900
agcagcgacc agaggcaggc ctgtaagaag cacgagctgt atgtcagctt ccgagacctg 960
ggctggcagg actggatcat cgcgcctgaa ggctacgccg cctactactg tgagggggag 1020
tgtgccttcc ctctgaactc ctacatgaac gccaccaacc acgccatcgt gcagacgctg 1080
gtccacttca tcaacccgga aacggtgccc aagccctgct gtgcgcccac gcagctcaat 1140
gccatctccg tcctctactt cgatgacagc tccaacgtca tcctgaagaa atacagaaac 1200
atggtggtcc gggcctgtgg ctgccac                                     1227

<210> 8
<211> 409
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the ?S293-M314 mutation and encoded by
      a Homo sapiens derived cDNA.

<400> 8
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              

Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Met Ala Asn Val Ala Glu Asn Ser Ser Ser Asp Gln
    290                 295                 300                 
Arg Gln Ala Cys Lys Lys His Glu Leu Tyr Val Ser Phe Arg Asp Leu
305                 310                 315                 320 
Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu Gly Tyr Ala Ala Tyr Tyr
                325                 330                 335     
Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn Ser Tyr Met Asn Ala Thr
            340                 345                 350         
Asn His Ala Ile Val Gln Thr Leu Val His Phe Ile Asn Pro Glu Thr
        355                 360                 365             
Val Pro Lys Pro Cys Cys Ala Pro Thr Gln Leu Asn Ala Ile Ser Val
    370                 375                 380                 
Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile Leu Lys Lys Tyr Arg Asn
385                 390                 395                 400 
Met Val Val Arg Ala Cys Gly Cys His
                405                 


<210> 9
<211> 1224
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the ?S293-M315 mutation.

<400> 9
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccgggcca acgtggcaga gaacagcagc 900
agcgaccaga ggcaggcctg taagaagcac gagctgtatg tcagcttccg agacctgggc 960
tggcaggact ggatcatcgc gcctgaaggc tacgccgcct actactgtga gggggagtgt 1020
gccttccctc tgaactccta catgaacgcc accaaccacg ccatcgtgca gacgctggtc 1080
cacttcatca acccggaaac ggtgcccaag ccctgctgtg cgcccacgca gctcaatgcc 1140
atctccgtcc tctacttcga tgacagctcc aacgtcatcc tgaagaaata cagaaacatg 1200
gtggtccggg cctgtggctg ccac                                        1224

<210> 10
<211> 408
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the ?S293-M315 mutation and encoded by
      a Homo sapiens derived cDNA.

<400> 10
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ala Asn Val Ala Glu Asn Ser Ser Ser Asp Gln Arg
    290                 295                 300                 
Gln Ala Cys Lys Lys His Glu Leu Tyr Val Ser Phe Arg Asp Leu Gly
305                 310                 315                 320 
Trp Gln Asp Trp Ile Ile Ala Pro Glu Gly Tyr Ala Ala Tyr Tyr Cys
                325                 330                 335     
Glu Gly Glu Cys Ala Phe Pro Leu Asn Ser Tyr Met Asn Ala Thr Asn
            340                 345                 350         
His Ala Ile Val Gln Thr Leu Val His Phe Ile Asn Pro Glu Thr Val
        355                 360                 365             
Pro Lys Pro Cys Cys Ala Pro Thr Gln Leu Asn Ala Ile Ser Val Leu
    370                 375                 380                 
Tyr Phe Asp Asp Ser Ser Asn Val Ile Leu Lys Lys Tyr Arg Asn Met
385                 390                 395                 400 
Val Val Arg Ala Cys Gly Cys His
                405             


<210> 11
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and L407K mutations.

<400> 11
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcaa gtacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 12
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and L407K mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 12
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Lys Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     

Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 13
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and F409K mutations.

<400> 13
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacaaggat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 14
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and F409K mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 14
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Lys Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 15
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and L417R mutations.

<400> 15
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatccg gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 16
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and L417R mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 16
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     

Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Arg Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 17
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and L382K mutations.

<400> 17
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgaaggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 18
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and L382K mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 18
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Lys Val His
    370                 375                 380                 

Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 19
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and L382N mutations.

<400> 19
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgaacgtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 20
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and L382N mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 20
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Asn Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 21
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and V383K mutations.

<400> 21
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctgaagc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 22
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and V383K mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 22
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     

Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Lys His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 23
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and I386R mutations.

<400> 23
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcaggaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 24
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and I386R mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 24
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Arg Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     

Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 25
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and I386R mutations.

<400> 25
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcaacaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 26
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and I386R mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 26
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Asn Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 27
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, V383K, L407K, and F409K
      mutations.

<400> 27
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctgaagc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcaa gtacaaggat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 28
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, V383K, L407K, and
      F409K mutations and encoded by a Homo sapiens
      derived cDNA.

<400> 28
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 

His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Lys His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Lys Tyr Lys Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 29
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, and W347D mutations.

<400> 29
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggacga catcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 30
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, and W347D mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 30
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Asp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 

Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 31
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the R299S, R314S, R421E, N422D, and R426E
      mutations.

<400> 31
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
gaagacatgg tggtcgaggc ctgtggctgc cac                              1293

<210> 32
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the R299S, R314S, R421E, N422D, and
      R426E mutations and encoded by a Homo sapiens
      derived cDNA.

<400> 32
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Glu Asp Met Val Val Glu Ala Cys Gly Cys His
            420                 425                 430     


<210> 33
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the S293C, R299S, and R314S mutations.

<400> 33
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccggtgca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 34
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the S293C, R299S, and R314S mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 34
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 

Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg Cys Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 35
<211> 876
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the native hBMP-7 signal peptide and prodomain
      alone due to a deletion mutation that removed the
      hBMP-7 mature form domain.

<400> 35
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcca cttccgcagc atccgg                           876

<210> 36
<211> 292
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the native hBMP-7 signal peptide and
      prodomain alone due to a deletion mutation that
      removed the hBMP-7 mature form domain.  This
      hBMP-7 precursor derived variant peptide chain is
      encoded by a Homo sapiens derived cDNA.

<400> 36
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val His Phe
        275                 280                 285             
Arg Ser Ile Arg
    290         


<210> 37
<211> 495
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the human growth hormone signal peptide instead of
      the native hBMP-7 secretory signal peptide and the
      R299S and R314S mutations in the hBMP-7 mature
      form domain; the hBMP-7 prodomain has been removed
      by deletion mutation.

<400> 37
atggctacag gctcccggac gtccctgctc ctggcttttg gcctgctctg cctgccctgg 60
cttcaagagg gatccgcctc cacggggagc aaacagagca gccagaaccg ctccaagacg 120
cccaagaacc aggaagccct gagcatggcc aacgtggcag agaacagcag cagcgaccag 180
aggcaggcct gtaagaagca cgagctgtat gtcagcttcc gagacctggg ctggcaggac 240
tggatcatcg cgcctgaagg ctacgccgcc tactactgtg agggggagtg tgccttccct 300
ctgaactcct acatgaacgc caccaaccac gccatcgtgc agacgctggt ccacttcatc 360
aacccggaaa cggtgcccaa gccctgctgt gcgcccacgc agctcaatgc catctccgtc 420
ctctacttcg atgacagctc caacgtcatc ctgaagaaat acagaaacat ggtggtccgg 480
gcctgtggct gccac                                                  495

<210> 38
<211> 165
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the human growth hormone signal peptide
      instead of the native hBMP-7 secretory signal
      peptide and the R299S and R314S mutations in the
      hBMP-7 mature form domain; the hBMP-7 prodomain
      has been removed by deletion mutation.  This
      hBMP-7 precursor derived variant peptide chain is
      encoded by a Homo sapiens derived cDNA.

<400> 38
Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu
 1               5                  10                  15      
Cys Leu Pro Trp Leu Gln Glu Gly Ser Ala Ser Thr Gly Ser Lys Gln
            20                  25                  30          
Ser Ser Gln Asn Arg Ser Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser
        35                  40                  45              
Met Ala Asn Val Ala Glu Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys
    50                  55                  60                  
Lys Lys His Glu Leu Tyr Val Ser Phe Arg Asp Leu Gly Trp Gln Asp
65                  70                  75                  80  
Trp Ile Ile Ala Pro Glu Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu
                85                  90                  95      
Cys Ala Phe Pro Leu Asn Ser Tyr Met Asn Ala Thr Asn His Ala Ile
            100                 105                 110         
Val Gln Thr Leu Val His Phe Ile Asn Pro Glu Thr Val Pro Lys Pro
        115                 120                 125             
Cys Cys Ala Pro Thr Gln Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp
    130                 135                 140                 
Asp Ser Ser Asn Val Ile Leu Lys Lys Tyr Arg Asn Met Val Val Arg
145                 150                 155                 160 
Ala Cys Gly Cys His
                165 


<210> 39
<211> 1263
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the Homo sapiens BMP-2 (hBMP-2) secretory signal
      peptide instead of the native hBMP-7 secretory
      signal peptide, the hBMP-2 prodomain instead of
      the native hBMP-7 prodomain, and the R299S and
      R314S mutations in the hBMP-7 mature form domain.

<400> 39
atggtggccg ggacccgctg tcttctagcg ttgctgcttc cccaggtcct cctgggcggc 60
gcggctggcc tcgttccgga gctgggccgc aggaagttcg cggcggcgtc gtcgggccgc 120
ccctcatccc agccctctga cgaggtcctg agcgagttcg agttgcggct gctcagcatg 180
ttcggcctga aacagagacc cacccccagc agggacgccg tggtgccccc ctacatgcta 240
gacctgtatc gcaggcactc aggtcagccg ggctcacccg ccccagacca ccggttggag 300
agggcagcca gccgagccaa cactgtgcgc agcttccacc atgaagaatc tttggaagaa 360
ctaccagaaa cgagtgggaa aacaacccgg agattcttct ttaatttaag ttctatcccc 420
acggaggagt ttatcacctc agcagagctt caggttttcc gagaacagat gcaagatgct 480
ttaggaaaca atagcagttt ccatcaccga attaatattt atgaaatcat aaaacctgca 540
acagccaact cgaaattccc cgtgaccaga cttttggaca ccaggttggt gaatcagaat 600
gcaagcaggt gggaaagttt tgatgtcacc cccgctgtga tgcggtggac tgcacaggga 660
cacgccaacc atggattcgt ggtggaagtg gcccacttgg aggagaaaca aggtgtctcc 720
aagagacatg ttaggataag caggtctttg caccaagatg aacacagctg gtcacagata 780
aggccattgc tagtaacttt tggccatgat ggaaaagggc atcctctcca caaaagagaa 840
aaaagatcta cggggagcaa acagagcagc cagaaccgct ccaagacgcc caagaaccag 900
gaagccctga gcatggccaa cgtggcagag aacagcagca gcgaccagag gcaggcctgt 960
aagaagcacg agctgtatgt cagcttccga gacctgggct ggcaggactg gatcatcgcg 1020
cctgaaggct acgccgccta ctactgtgag ggggagtgtg ccttccctct gaactcctac 1080
atgaacgcca ccaaccacgc catcgtgcag acgctggtcc acttcatcaa cccggaaacg 1140
gtgcccaagc cctgctgtgc gcccacgcag ctcaatgcca tctccgtcct ctacttcgat 1200
gacagctcca acgtcatcct gaagaaatac agaaacatgg tggtccgggc ctgtggctgc 1260
cac                                                               1263

<210> 40
<211> 421
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the Homo sapiens BMP-2 (hBMP-2)
      secretory signal peptide instead of the native
      hBMP-7 secretory signal peptide, the hBMP-2
      prodomain instead of the native hBMP-7 prodomain,
      and the R299S and R314S mutations in the hBMP-7
      mature form domain.  This hBMP-7 precursor derived
      variant peptide chain is encoded by a Homo sapiens
      derived cDNA.

<400> 40
Met Val Ala Gly Thr Arg Cys Leu Leu Ala Leu Leu Leu Pro Gln Val
 1               5                  10                  15      
Leu Leu Gly Gly Ala Ala Gly Leu Val Pro Glu Leu Gly Arg Arg Lys
            20                  25                  30          
Phe Ala Ala Ala Ser Ser Gly Arg Pro Ser Ser Gln Pro Ser Asp Glu
        35                  40                  45              
Val Leu Ser Glu Phe Glu Leu Arg Leu Leu Ser Met Phe Gly Leu Lys
    50                  55                  60                  
Gln Arg Pro Thr Pro Ser Arg Asp Ala Val Val Pro Pro Tyr Met Leu
65                  70                  75                  80  
Asp Leu Tyr Arg Arg His Ser Gly Gln Pro Gly Ser Pro Ala Pro Asp
                85                  90                  95      

His Arg Leu Glu Arg Ala Ala Ser Arg Ala Asn Thr Val Arg Ser Phe
            100                 105                 110         
His His Glu Glu Ser Leu Glu Glu Leu Pro Glu Thr Ser Gly Lys Thr
        115                 120                 125             
Thr Arg Arg Phe Phe Phe Asn Leu Ser Ser Ile Pro Thr Glu Glu Phe
    130                 135                 140                 
Ile Thr Ser Ala Glu Leu Gln Val Phe Arg Glu Gln Met Gln Asp Ala
145                 150                 155                 160 
Leu Gly Asn Asn Ser Ser Phe His His Arg Ile Asn Ile Tyr Glu Ile
                165                 170                 175     
Ile Lys Pro Ala Thr Ala Asn Ser Lys Phe Pro Val Thr Arg Leu Leu
            180                 185                 190         
Asp Thr Arg Leu Val Asn Gln Asn Ala Ser Arg Trp Glu Ser Phe Asp
        195                 200                 205             
Val Thr Pro Ala Val Met Arg Trp Thr Ala Gln Gly His Ala Asn His
    210                 215                 220                 
Gly Phe Val Val Glu Val Ala His Leu Glu Glu Lys Gln Gly Val Ser
225                 230                 235                 240 
Lys Arg His Val Arg Ile Ser Arg Ser Leu His Gln Asp Glu His Ser
                245                 250                 255     
Trp Ser Gln Ile Arg Pro Leu Leu Val Thr Phe Gly His Asp Gly Lys
            260                 265                 270         
Gly His Pro Leu His Lys Arg Glu Lys Arg Ser Thr Gly Ser Lys Gln
        275                 280                 285             
Ser Ser Gln Asn Arg Ser Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser
    290                 295                 300                 
Met Ala Asn Val Ala Glu Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys
305                 310                 315                 320 
Lys Lys His Glu Leu Tyr Val Ser Phe Arg Asp Leu Gly Trp Gln Asp
                325                 330                 335     
Trp Ile Ile Ala Pro Glu Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu
            340                 345                 350         
Cys Ala Phe Pro Leu Asn Ser Tyr Met Asn Ala Thr Asn His Ala Ile
        355                 360                 365             
Val Gln Thr Leu Val His Phe Ile Asn Pro Glu Thr Val Pro Lys Pro
    370                 375                 380                 
Cys Cys Ala Pro Thr Gln Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp
385                 390                 395                 400 
Asp Ser Ser Asn Val Ile Leu Lys Lys Tyr Arg Asn Met Val Val Arg
                405                 410                 415     
Ala Cys Gly Cys His
            420     


<210> 41
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the H287R, R299S, R314S, R421E, N422D, and R426E
      mutations.

<400> 41
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtccg cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
gaagacatgg tggtcgaggc ctgtggctgc cac                              1293

<210> 42
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the H287R, R299S, R314S, R421E, N422D,
      and R426E mutations and encoded by a Homo sapiens
      derived cDNA.

<400> 42
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 

His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val Arg Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Glu Asp Met Val Val Glu Ala Cys Gly Cys His
            420                 425                 430     


<210> 43
<211> 1263
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the Homo sapiens BMP-2 (hBMP-2) secretory signal
      peptide instead of the native hBMP-7 secretory
      signal peptide, the hBMP-2 prodomain instead of
      the native hBMP-7 prodomain, and the R299S, R314S,
      R421E, N422D and R426E mutations in the hBMP-7
      mature form domain.

<400> 43
atggtggccg ggacccgctg tcttctagcg ttgctgcttc cccaggtcct cctgggcggc 60
gcggctggcc tcgttccgga gctgggccgc aggaagttcg cggcggcgtc gtcgggccgc 120
ccctcatccc agccctctga cgaggtcctg agcgagttcg agttgcggct gctcagcatg 180
ttcggcctga aacagagacc cacccccagc agggacgccg tggtgccccc ctacatgcta 240
gacctgtatc gcaggcactc aggtcagccg ggctcacccg ccccagacca ccggttggag 300
agggcagcca gccgagccaa cactgtgcgc agcttccacc atgaagaatc tttggaagaa 360
ctaccagaaa cgagtgggaa aacaacccgg agattcttct ttaatttaag ttctatcccc 420
acggaggagt ttatcacctc agcagagctt caggttttcc gagaacagat gcaagatgct 480
ttaggaaaca atagcagttt ccatcaccga attaatattt atgaaatcat aaaacctgca 540
acagccaact cgaaattccc cgtgaccaga cttttggaca ccaggttggt gaatcagaat 600
gcaagcaggt gggaaagttt tgatgtcacc cccgctgtga tgcggtggac tgcacaggga 660
cacgccaacc atggattcgt ggtggaagtg gcccacttgg aggagaaaca aggtgtctcc 720
aagagacatg ttaggataag caggtctttg caccaagatg aacacagctg gtcacagata 780
aggccattgc tagtaacttt tggccatgat ggaaaagggc atcctctcca caaaagagaa 840
aaaagatcta cggggagcaa acagagcagc cagaaccgct ccaagacgcc caagaaccag 900
gaagccctga gcatggccaa cgtggcagag aacagcagca gcgaccagag gcaggcctgt 960
aagaagcacg agctgtatgt cagcttccga gacctgggct ggcaggactg gatcatcgcg 1020
cctgaaggct acgccgccta ctactgtgag ggggagtgtg ccttccctct gaactcctac 1080
atgaacgcca ccaaccacgc catcgtgcag acgctggtcc acttcatcaa cccggaaacg 1140
gtgcccaagc cctgctgtgc gcccacgcag ctcaatgcca tctccgtcct ctacttcgat 1200
gacagctcca acgtcatcct gaagaaatac gaagacatgg tggtcgaggc ctgtggctgc 1260
cac                                                               1263

<210> 44
<211> 421
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the Homo sapiens BMP-2 (hBMP-2)
      secretory signal peptide instead of the native
      hBMP-7 secretory signal peptide, the hBMP-2
      prodomain instead of the native hBMP-7 prodomain,
      and the R299S, R314S, R421E, N422D and R426E
      mutations in the hBMP-7 mature form domain.  This
      hBMP-7 precursor derived variant peptide chain is
      encoded by a Homo sapiens derived cDNA.

<400> 44
Met Val Ala Gly Thr Arg Cys Leu Leu Ala Leu Leu Leu Pro Gln Val
 1               5                  10                  15      
Leu Leu Gly Gly Ala Ala Gly Leu Val Pro Glu Leu Gly Arg Arg Lys
            20                  25                  30          
Phe Ala Ala Ala Ser Ser Gly Arg Pro Ser Ser Gln Pro Ser Asp Glu
        35                  40                  45              
Val Leu Ser Glu Phe Glu Leu Arg Leu Leu Ser Met Phe Gly Leu Lys
    50                  55                  60                  
Gln Arg Pro Thr Pro Ser Arg Asp Ala Val Val Pro Pro Tyr Met Leu
65                  70                  75                  80  
Asp Leu Tyr Arg Arg His Ser Gly Gln Pro Gly Ser Pro Ala Pro Asp
                85                  90                  95      
His Arg Leu Glu Arg Ala Ala Ser Arg Ala Asn Thr Val Arg Ser Phe
            100                 105                 110         
His His Glu Glu Ser Leu Glu Glu Leu Pro Glu Thr Ser Gly Lys Thr
        115                 120                 125             
Thr Arg Arg Phe Phe Phe Asn Leu Ser Ser Ile Pro Thr Glu Glu Phe
    130                 135                 140                 
Ile Thr Ser Ala Glu Leu Gln Val Phe Arg Glu Gln Met Gln Asp Ala
145                 150                 155                 160 
Leu Gly Asn Asn Ser Ser Phe His His Arg Ile Asn Ile Tyr Glu Ile
                165                 170                 175     
Ile Lys Pro Ala Thr Ala Asn Ser Lys Phe Pro Val Thr Arg Leu Leu
            180                 185                 190         
Asp Thr Arg Leu Val Asn Gln Asn Ala Ser Arg Trp Glu Ser Phe Asp
        195                 200                 205             
Val Thr Pro Ala Val Met Arg Trp Thr Ala Gln Gly His Ala Asn His
    210                 215                 220                 
Gly Phe Val Val Glu Val Ala His Leu Glu Glu Lys Gln Gly Val Ser
225                 230                 235                 240 
Lys Arg His Val Arg Ile Ser Arg Ser Leu His Gln Asp Glu His Ser
                245                 250                 255     
Trp Ser Gln Ile Arg Pro Leu Leu Val Thr Phe Gly His Asp Gly Lys
            260                 265                 270         
Gly His Pro Leu His Lys Arg Glu Lys Arg Ser Thr Gly Ser Lys Gln
        275                 280                 285             
Ser Ser Gln Asn Arg Ser Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser
    290                 295                 300                 
Met Ala Asn Val Ala Glu Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys
305                 310                 315                 320 
Lys Lys His Glu Leu Tyr Val Ser Phe Arg Asp Leu Gly Trp Gln Asp
                325                 330                 335     
Trp Ile Ile Ala Pro Glu Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu
            340                 345                 350         
Cys Ala Phe Pro Leu Asn Ser Tyr Met Asn Ala Thr Asn His Ala Ile
        355                 360                 365             
Val Gln Thr Leu Val His Phe Ile Asn Pro Glu Thr Val Pro Lys Pro
    370                 375                 380                 
Cys Cys Ala Pro Thr Gln Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp
385                 390                 395                 400 
Asp Ser Ser Asn Val Ile Leu Lys Lys Tyr Glu Asp Met Val Val Glu
                405                 410                 415     
Ala Cys Gly Cys His
            420     


<210> 45
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the H287T, R299S, and R314S mutations.

<400> 45
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtccg cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 46
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the H287T, R299S, and R314S mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 46
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 
Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val Arg Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     


<210> 47
<211> 1293
<212> DNA
<213> Artificial Sequence

<220> 
<223> Homo sapiens derived cDNA encoding a hBMP-7
      precursor derived variant peptide chain comprising
      the H287R, R299S, and R314S mutations.

<400> 47
atgcacgtgc gctcactgcg agctgcggcg ccgcacagct tcgtggcgct ctgggcaccc 60
ctgttcctgc tgcgctccgc cctggccgac ttcagcctgg acaacgaggt gcactcgagc 120
ttcatccacc ggcgcctccg cagccaggag cggcgggaga tgcagcgcga gatcctctcc 180
attttgggct tgccccaccg cccgcgcccg cacctccagg gcaagcacaa ctcggcaccc 240
atgttcatgc tggacctgta caacgccatg gcggtggagg agggcggcgg gcccggcggc 300
cagggcttct cctaccccta caaggccgtc ttcagtaccc agggcccccc tctggccagc 360
ctgcaagata gccatttcct caccgacgcc gacatggtca tgagcttcgt caacctcgtg 420
gaacatgaca aggaattctt ccacccacgc taccaccatc gagagttccg gtttgatctt 480
tccaagatcc cagaagggga agctgtcacg gcagccgaat tccggatcta caaggactac 540
atccgggaac gcttcgacaa tgagacgttc cggatcagcg tttatcaggt gctccaggag 600
cacttgggca gggaatcgga tctcttcctg ctcgacagcc gtaccctctg ggcctcggag 660
gagggctggc tggtgtttga catcacagcc accagcaacc actgggtggt caatccgcgg 720
cacaacctgg gcctgcagct ctcggtggag acgctggatg ggcagagcat caaccccaag 780
ttggcgggcc tgattgggcg gcacgggccc cagaacaagc agcccttcat ggtggctttc 840
ttcaaggcca cggaggtcac cttccgcagc atccggtcca cggggagcaa acagagcagc 900
cagaaccgct ccaagacgcc caagaaccag gaagccctga gcatggccaa cgtggcagag 960
aacagcagca gcgaccagag gcaggcctgt aagaagcacg agctgtatgt cagcttccga 1020
gacctgggct ggcaggactg gatcatcgcg cctgaaggct acgccgccta ctactgtgag 1080
ggggagtgtg ccttccctct gaactcctac atgaacgcca ccaaccacgc catcgtgcag 1140
acgctggtcc acttcatcaa cccggaaacg gtgcccaagc cctgctgtgc gcccacgcag 1200
ctcaatgcca tctccgtcct ctacttcgat gacagctcca acgtcatcct gaagaaatac 1260
agaaacatgg tggtccgggc ctgtggctgc cac                              1293

<210> 48
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBMP-7 precursor derived variant peptide chain
      comprising the H287R, R299S, and R314S mutations
      and encoded by a Homo sapiens derived cDNA.

<400> 48
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
 1               5                  10                  15      
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala Asp Phe Ser
            20                  25                  30          
Leu Asp Asn Glu Val His Ser Ser Phe Ile His Arg Arg Leu Arg Ser
        35                  40                  45              
Gln Glu Arg Arg Glu Met Gln Arg Glu Ile Leu Ser Ile Leu Gly Leu
    50                  55                  60                  
Pro His Arg Pro Arg Pro His Leu Gln Gly Lys His Asn Ser Ala Pro
65                  70                  75                  80  
Met Phe Met Leu Asp Leu Tyr Asn Ala Met Ala Val Glu Glu Gly Gly
                85                  90                  95      
Gly Pro Gly Gly Gln Gly Phe Ser Tyr Pro Tyr Lys Ala Val Phe Ser
            100                 105                 110         
Thr Gln Gly Pro Pro Leu Ala Ser Leu Gln Asp Ser His Phe Leu Thr
        115                 120                 125             
Asp Ala Asp Met Val Met Ser Phe Val Asn Leu Val Glu His Asp Lys
    130                 135                 140                 
Glu Phe Phe His Pro Arg Tyr His His Arg Glu Phe Arg Phe Asp Leu
145                 150                 155                 160 

Ser Lys Ile Pro Glu Gly Glu Ala Val Thr Ala Ala Glu Phe Arg Ile
                165                 170                 175     
Tyr Lys Asp Tyr Ile Arg Glu Arg Phe Asp Asn Glu Thr Phe Arg Ile
            180                 185                 190         
Ser Val Tyr Gln Val Leu Gln Glu His Leu Gly Arg Glu Ser Asp Leu
        195                 200                 205             
Phe Leu Leu Asp Ser Arg Thr Leu Trp Ala Ser Glu Glu Gly Trp Leu
    210                 215                 220                 
Val Phe Asp Ile Thr Ala Thr Ser Asn His Trp Val Val Asn Pro Arg
225                 230                 235                 240 
His Asn Leu Gly Leu Gln Leu Ser Val Glu Thr Leu Asp Gly Gln Ser
                245                 250                 255     
Ile Asn Pro Lys Leu Ala Gly Leu Ile Gly Arg His Gly Pro Gln Asn
            260                 265                 270         
Lys Gln Pro Phe Met Val Ala Phe Phe Lys Ala Thr Glu Val Thr Phe
        275                 280                 285             
Arg Ser Ile Arg Ser Thr Gly Ser Lys Gln Ser Ser Gln Asn Arg Ser
    290                 295                 300                 
Lys Thr Pro Lys Asn Gln Glu Ala Leu Ser Met Ala Asn Val Ala Glu
305                 310                 315                 320 
Asn Ser Ser Ser Asp Gln Arg Gln Ala Cys Lys Lys His Glu Leu Tyr
                325                 330                 335     
Val Ser Phe Arg Asp Leu Gly Trp Gln Asp Trp Ile Ile Ala Pro Glu
            340                 345                 350         
Gly Tyr Ala Ala Tyr Tyr Cys Glu Gly Glu Cys Ala Phe Pro Leu Asn
        355                 360                 365             
Ser Tyr Met Asn Ala Thr Asn His Ala Ile Val Gln Thr Leu Val His
    370                 375                 380                 
Phe Ile Asn Pro Glu Thr Val Pro Lys Pro Cys Cys Ala Pro Thr Gln
385                 390                 395                 400 
Leu Asn Ala Ile Ser Val Leu Tyr Phe Asp Asp Ser Ser Asn Val Ile
                405                 410                 415     
Leu Lys Lys Tyr Arg Asn Met Val Val Arg Ala Cys Gly Cys His
            420                 425                 430     

