                         SEQUENCE LISTING

<110>  LeBowitz, et al
 
<120>  Methods for Treating Pompe Disease

<130>  2008266-0002 (SYM-013PC)

<140>  To be assigned
<141>  2007-11-13

<160>  8     

<170>  PatentIn version 3.2

<210>  1
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primers

<400>  1
ggaattccaa ccatgggagt gaggcacccg ccc                                  33


<210>  2
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primers

<400>  2
gctctagact aacaccagct gacgagaaac tgc                                  33


<210>  3
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A DNA cassette for the production of recombinant GILT-tagged GAA 
       ZC-701

<400>  3
gaattcacac caatgggaat cccaatgggg aagtcgatgc tggtgcttct caccttcttg     60

gccttcgcct cgtgctgcat tgctgctctg tgcggcgggg agctggtgga caccctccag    120

ttcgtctgtg gggaccgcgg cttctacttc agcaggcccg caagccgtgt gagccgtcgc    180

agccgtggca tcgttgagga gtgctgtttc cgcagctgtg acctggccct cctggagacg    240

tactgtgcta cccccgccaa gtccgagggc gcgccg                              276


<210>  4
<211>  971
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cassette 701 was digested with EcoRI and XbaI, blunted by 
       treatment with Klenow DNA polymerase

<400>  4

Met Gly Ile Pro Met Gly Lys Ser Met Leu Val Leu Leu Thr Phe Leu 
1               5                   10                  15      


Ala Phe Ala Ser Cys Cys Ile Ala Ala Leu Cys Gly Gly Glu Leu Val 
            20                  25                  30          


Asp Thr Leu Gln Phe Val Cys Gly Asp Arg Gly Phe Tyr Phe Ser Arg 
        35                  40                  45              


Pro Ala Ser Arg Val Ser Arg Arg Ser Arg Gly Ile Val Glu Glu Cys 
    50                  55                  60                  


Cys Phe Arg Ser Cys Asp Leu Ala Leu Leu Glu Thr Tyr Cys Ala Thr 
65                  70                  75                  80  


Pro Ala Lys Ser Glu Gly Ala Pro Ala His Pro Gly Arg Pro Arg Ala 
                85                  90                  95      


Val Pro Thr Gln Cys Asp Val Pro Pro Asn Ser Arg Phe Asp Cys Ala 
            100                 105                 110         


Pro Asp Lys Ala Ile Thr Gln Glu Gln Cys Glu Ala Arg Gly Cys Cys 
        115                 120                 125             


Tyr Ile Pro Ala Lys Gln Gly Leu Gln Gly Ala Gln Met Gly Gln Pro 
    130                 135                 140                 


Trp Cys Phe Phe Pro Pro Ser Tyr Pro Ser Tyr Lys Leu Glu Asn Leu 
145                 150                 155                 160 


Ser Ser Ser Glu Met Gly Tyr Thr Ala Thr Leu Thr Arg Thr Thr Pro 
                165                 170                 175     


Thr Phe Phe Pro Lys Asp Ile Leu Thr Leu Arg Leu Asp Val Met Met 
            180                 185                 190         


Glu Thr Glu Asn Arg Leu His Phe Thr Ile Lys Asp Pro Ala Asn Arg 
        195                 200                 205             


Arg Tyr Glu Val Pro Leu Glu Thr Pro Arg Val His Ser Arg Ala Pro 
    210                 215                 220                 


Ser Pro Leu Tyr Ser Val Glu Phe Ser Glu Glu Pro Phe Gly Val Ile 
225                 230                 235                 240 


Val His Arg Gln Leu Asp Gly Arg Val Leu Leu Asn Thr Thr Val Ala 
                245                 250                 255     


Pro Leu Phe Phe Ala Asp Gln Phe Leu Gln Leu Ser Thr Ser Leu Pro 
            260                 265                 270         


Ser Gln Tyr Ile Thr Gly Leu Ala Glu His Leu Ser Pro Leu Met Leu 
        275                 280                 285             


Ser Thr Ser Trp Thr Arg Ile Thr Leu Trp Asn Arg Asp Leu Ala Pro 
    290                 295                 300                 


Thr Pro Gly Ala Asn Leu Tyr Gly Ser His Pro Phe Tyr Leu Ala Leu 
305                 310                 315                 320 


Glu Asp Gly Gly Ser Ala His Gly Val Phe Leu Leu Asn Ser Asn Ala 
                325                 330                 335     


Met Asp Val Val Leu Gln Pro Ser Pro Ala Leu Ser Trp Arg Ser Thr 
            340                 345                 350         


Gly Gly Ile Leu Asp Val Tyr Ile Phe Leu Gly Pro Glu Pro Lys Ser 
        355                 360                 365             


Val Val Gln Gln Tyr Leu Asp Val Val Gly Tyr Pro Phe Met Pro Pro 
    370                 375                 380                 


Tyr Trp Gly Leu Gly Phe His Leu Cys Arg Trp Gly Tyr Ser Ser Thr 
385                 390                 395                 400 


Ala Ile Thr Arg Gln Val Val Glu Asn Met Thr Arg Ala His Phe Pro 
                405                 410                 415     


Leu Asp Val Gln Trp Asn Asp Leu Asp Tyr Met Asp Ser Arg Arg Asp 
            420                 425                 430         


Phe Thr Phe Asn Lys Asp Gly Phe Arg Asp Phe Pro Ala Met Val Gln 
        435                 440                 445             


Glu Leu His Gln Gly Gly Arg Arg Tyr Met Met Ile Val Asp Pro Ala 
    450                 455                 460                 


Ile Ser Ser Ser Gly Pro Ala Gly Ser Tyr Arg Pro Tyr Asp Glu Gly 
465                 470                 475                 480 


Leu Arg Arg Gly Val Phe Ile Thr Asn Glu Thr Gly Gln Pro Leu Ile 
                485                 490                 495     


Gly Lys Val Trp Pro Gly Ser Thr Ala Phe Pro Asp Phe Thr Asn Pro 
            500                 505                 510         


Thr Ala Leu Ala Trp Trp Glu Asp Met Val Ala Glu Phe His Asp Gln 
        515                 520                 525             


Val Pro Phe Asp Gly Met Trp Ile Asp Met Asn Glu Pro Ser Asn Phe 
    530                 535                 540                 


Ile Arg Gly Ser Glu Asp Gly Cys Pro Asn Asn Glu Leu Glu Asn Pro 
545                 550                 555                 560 


Pro Tyr Val Pro Gly Val Val Gly Gly Thr Leu Gln Ala Ala Thr Ile 
                565                 570                 575     


Cys Ala Ser Ser His Gln Phe Leu Ser Thr His Tyr Asn Leu His Asn 
            580                 585                 590         


Leu Tyr Gly Leu Thr Glu Ala Ile Ala Ser His Arg Ala Leu Val Lys 
        595                 600                 605             


Ala Arg Gly Thr Arg Pro Phe Val Ile Ser Arg Ser Thr Phe Ala Gly 
    610                 615                 620                 


His Gly Arg Tyr Ala Gly His Trp Thr Gly Asp Val Trp Ser Ser Trp 
625                 630                 635                 640 


Glu Gln Leu Ala Ser Ser Val Pro Glu Ile Leu Gln Phe Asn Leu Leu 
                645                 650                 655     


Gly Val Pro Leu Val Gly Ala Asp Val Cys Gly Phe Leu Gly Asn Thr 
            660                 665                 670         


Ser Glu Glu Leu Cys Val Arg Trp Thr Gln Leu Gly Ala Phe Tyr Pro 
        675                 680                 685             


Phe Met Arg Asn His Asn Ser Leu Leu Ser Leu Pro Gln Glu Pro Tyr 
    690                 695                 700                 


Ser Phe Ser Glu Pro Ala Gln Gln Ala Met Arg Lys Ala Leu Thr Leu 
705                 710                 715                 720 


Arg Tyr Ala Leu Leu Pro His Leu Tyr Thr Leu Phe His Gln Ala His 
                725                 730                 735     


Val Ala Gly Glu Thr Val Ala Arg Pro Leu Phe Leu Glu Phe Pro Lys 
            740                 745                 750         


Asp Ser Ser Thr Trp Thr Val Asp His Gln Leu Leu Trp Gly Glu Ala 
        755                 760                 765             


Leu Leu Ile Thr Pro Val Leu Gln Ala Gly Lys Ala Glu Val Thr Gly 
    770                 775                 780                 


Tyr Phe Pro Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val Pro Ile Glu 
785                 790                 795                 800 


Ala Leu Gly Ser Leu Pro Pro Pro Pro Ala Ala Pro Arg Glu Pro Ala 
                805                 810                 815     


Ile His Ser Glu Gly Gln Trp Val Thr Leu Pro Ala Pro Leu Asp Thr 
            820                 825                 830         


Ile Asn Val His Leu Arg Ala Gly Tyr Ile Ile Pro Leu Gln Gly Pro 
        835                 840                 845             


Gly Leu Thr Thr Thr Glu Ser Arg Gln Gln Pro Met Ala Leu Ala Val 
    850                 855                 860                 


Ala Leu Thr Lys Gly Gly Glu Ala Arg Gly Glu Leu Phe Trp Asp Asp 
865                 870                 875                 880 


Gly Glu Ser Leu Glu Val Leu Glu Arg Gly Ala Tyr Thr Gln Val Ile 
                885                 890                 895     


Phe Leu Ala Arg Asn Asn Thr Ile Val Asn Glu Leu Val Arg Val Thr 
            900                 905                 910         


Ser Glu Gly Ala Gly Leu Gln Leu Gln Lys Val Thr Val Leu Gly Val 
        915                 920                 925             


Ala Thr Ala Pro Gln Gln Val Leu Ser Asn Gly Val Pro Val Ser Asn 
    930                 935                 940                 


Phe Thr Tyr Ser Pro Asp Thr Lys Val Leu Asp Ile Cys Val Ser Leu 
945                 950                 955                 960 


Leu Met Gly Glu Gln Phe Leu Val Ser Trp Cys 
                965                 970     


<210>  5
<211>  2040
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  His-Biotin-Ci-Mpr Domains 10-13

<400>  5
atgggaatcc caatggggaa gtcgatgctg gtgcttctca ccttcttggc cttcgcctcg     60

tgctgcattg ctgctggcgc gccgaccggt caccatcacc atcaccacgc gccgggcctg    120

aacgacatct tcgaggccca gaagatcgag tggcacgaac ctttcgatct gactgaatgt    180

tcattcaaag atggggctgg caactccttc gacctctcgt ccctgtcaag gtacagtgac    240

aactgggaag ccatcactgg gacgggggac ccggagcact acctcatcaa tgtctgcaag    300

tctctggccc cgcaggctgg cactgagccg tgccctccag aagcagccgc gtgtctgctg    360

ggtggctcca agcccgtgaa cctcggcagg gtaagggacg gacctcagtg gagagatggc    420

ataattgtcc tgaaatacgt tgatggcgac ttatgtccag atgggattcg gaaaaagtca    480

accaccatcc gattcacctg cagcgagagc caagtgaact ccaggcccat gttcatcagc    540

gccgtggagg actgtgagta cacctttgcc tggcccacag ccacagcctg tcccatgaag    600

agcaacgagc atgatgactg ccaggtcacc aacccaagca caggacacct gtttgatctg    660

agctccttaa gtggcagggc gggattcaca gctgcttaca gcgagaaggg gttggtttac    720

atgagcatct gtggggagaa tgaaaactgc cctcctggcg tgggggcctg ctttggacag    780

accaggatta gcgtgggcaa ggccaacaag aggctgagat acgtggacca ggtcctgcag    840

ctggtgtaca aggatgggtc cccttgtccc tccaaatccg gcctgagcta taagagtgtg    900

atcagtttcg tgtgcaggcc tgaggccggg ccaaccaata ggcccatgct catctccctg    960

gacaagcaga catgcactct cttcttctcc tggcacacgc cgctggcctg cgagcaagcg   1020

accgaatgtt ccgtgaggaa tggaagctct attgttgact tgtctcccct tattcatcgc   1080

actggtggtt atgaggctta tgatgagagt gaggatgatg cctccgatac caaccctgat   1140

ttctacatca atatttgtca gccactaaat cccatgcacg gagtgccctg tcctgccgga   1200

gccgctgtgt gcaaagttcc tattgatggt ccccccatag atatcggccg ggtagcagga   1260

ccaccaatac tcaatccaat agcaaatgag atttacttga attttgaaag cagtactcct   1320

tgcttagcgg acaagcattt caactacacc tcgctcatcg cgtttcactg taagagaggt   1380

gtgagcatgg gaacgcctaa gctgttaagg accagcgagt gcgactttgt gttcgaatgg   1440

gagactcctg tcgtctgtcc tgatgaagtg aggatggatg gctgtaccct gacagatgag   1500

cagctcctct acagcttcaa cttgtccagc ctttccacga gcacctttaa ggtgactcgc   1560

gactcgcgca cctacagcgt tggggtgtgc acctttgcag tcgggccaga acaaggaggc   1620

tgtaaggacg gaggagtctg tctgctctca ggcaccaagg gggcatcctt tggacggctg   1680

caatcaatga aactggatta caggcaccag gatgaagcgg tcgttttaag ttacgtgaat   1740

ggtgatcgtt gccctccaga aaccgatgac ggcgtcccct gtgtcttccc cttcatattc   1800

aatgggaaga gctacgagga gtgcatcata gagagcaggg cgaagctgtg gtgtagcaca   1860

actgcggact acgacagaga ccacgagtgg ggcttctgca gacactcaaa cagctaccgg   1920

acatccagca tcatatttaa gtgtgatgaa gatgaggaca ttgggaggcc acaagtcttc   1980

agtgaagtgc gtgggtgtga tgtgacattt gagtggaaaa caaaagttgt ctgcccttga   2040


<210>  6
<211>  679
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-Biotin-Ci-Mpr Domains 10-13 protein sequences

<400>  6

Met Gly Ile Pro Met Gly Lys Ser Met Leu Val Leu Leu Thr Phe Leu 
1               5                   10                  15      


Ala Phe Ala Ser Cys Cys Ile Ala Ala Gly Ala Pro Thr Gly His His 
            20                  25                  30          


His His His His Ala Pro Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
        35                  40                  45              


Ile Glu Trp His Glu Pro Phe Asp Leu Thr Glu Cys Ser Phe Lys Asp 
    50                  55                  60                  


Gly Ala Gly Asn Ser Phe Asp Leu Ser Ser Leu Ser Arg Tyr Ser Asp 
65                  70                  75                  80  


Asn Trp Glu Ala Ile Thr Gly Thr Gly Asp Pro Glu His Tyr Leu Ile 
                85                  90                  95      


Asn Val Cys Lys Ser Leu Ala Pro Gln Ala Gly Thr Glu Pro Cys Pro 
            100                 105                 110         


Pro Glu Ala Ala Ala Cys Leu Leu Gly Gly Ser Lys Pro Val Asn Leu 
        115                 120                 125             


Gly Arg Val Arg Asp Gly Pro Gln Trp Arg Asp Gly Ile Ile Val Leu 
    130                 135                 140                 


Lys Tyr Val Asp Gly Asp Leu Cys Pro Asp Gly Ile Arg Lys Lys Ser 
145                 150                 155                 160 


Thr Thr Ile Arg Phe Thr Cys Ser Glu Ser Gln Val Asn Ser Arg Pro 
                165                 170                 175     


Met Phe Ile Ser Ala Val Glu Asp Cys Glu Tyr Thr Phe Ala Trp Pro 
            180                 185                 190         


Thr Ala Thr Ala Cys Pro Met Lys Ser Asn Glu His Asp Asp Cys Gln 
        195                 200                 205             


Val Thr Asn Pro Ser Thr Gly His Leu Phe Asp Leu Ser Ser Leu Ser 
    210                 215                 220                 


Gly Arg Ala Gly Phe Thr Ala Ala Tyr Ser Glu Lys Gly Leu Val Tyr 
225                 230                 235                 240 


Met Ser Ile Cys Gly Glu Asn Glu Asn Cys Pro Pro Gly Val Gly Ala 
                245                 250                 255     


Cys Phe Gly Gln Thr Arg Ile Ser Val Gly Lys Ala Asn Lys Arg Leu 
            260                 265                 270         


Arg Tyr Val Asp Gln Val Leu Gln Leu Val Tyr Lys Asp Gly Ser Pro 
        275                 280                 285             


Cys Pro Ser Lys Ser Gly Leu Ser Tyr Lys Ser Val Ile Ser Phe Val 
    290                 295                 300                 


Cys Arg Pro Glu Ala Gly Pro Thr Asn Arg Pro Met Leu Ile Ser Leu 
305                 310                 315                 320 


Asp Lys Gln Thr Cys Thr Leu Phe Phe Ser Trp His Thr Pro Leu Ala 
                325                 330                 335     


Cys Glu Gln Ala Thr Glu Cys Ser Val Arg Asn Gly Ser Ser Ile Val 
            340                 345                 350         


Asp Leu Ser Pro Leu Ile His Arg Thr Gly Gly Tyr Glu Ala Tyr Asp 
        355                 360                 365             


Glu Ser Glu Asp Asp Ala Ser Asp Thr Asn Pro Asp Phe Tyr Ile Asn 
    370                 375                 380                 


Ile Cys Gln Pro Leu Asn Pro Met His Gly Val Pro Cys Pro Ala Gly 
385                 390                 395                 400 


Ala Ala Val Cys Lys Val Pro Ile Asp Gly Pro Pro Ile Asp Ile Gly 
                405                 410                 415     


Arg Val Ala Gly Pro Pro Ile Leu Asn Pro Ile Ala Asn Glu Ile Tyr 
            420                 425                 430         


Leu Asn Phe Glu Ser Ser Thr Pro Cys Leu Ala Asp Lys His Phe Asn 
        435                 440                 445             


Tyr Thr Ser Leu Ile Ala Phe His Cys Lys Arg Gly Val Ser Met Gly 
    450                 455                 460                 


Thr Pro Lys Leu Leu Arg Thr Ser Glu Cys Asp Phe Val Phe Glu Trp 
465                 470                 475                 480 


Glu Thr Pro Val Val Cys Pro Asp Glu Val Arg Met Asp Gly Cys Thr 
                485                 490                 495     


Leu Thr Asp Glu Gln Leu Leu Tyr Ser Phe Asn Leu Ser Ser Leu Ser 
            500                 505                 510         


Thr Ser Thr Phe Lys Val Thr Arg Asp Ser Arg Thr Tyr Ser Val Gly 
        515                 520                 525             


Val Cys Thr Phe Ala Val Gly Pro Glu Gln Gly Gly Cys Lys Asp Gly 
    530                 535                 540                 


Gly Val Cys Leu Leu Ser Gly Thr Lys Gly Ala Ser Phe Gly Arg Leu 
545                 550                 555                 560 


Gln Ser Met Lys Leu Asp Tyr Arg His Gln Asp Glu Ala Val Val Leu 
                565                 570                 575     


Ser Tyr Val Asn Gly Asp Arg Cys Pro Pro Glu Thr Asp Asp Gly Val 
            580                 585                 590         


Pro Cys Val Phe Pro Phe Ile Phe Asn Gly Lys Ser Tyr Glu Glu Cys 
        595                 600                 605             


Ile Ile Glu Ser Arg Ala Lys Leu Trp Cys Ser Thr Thr Ala Asp Tyr 
    610                 615                 620                 


Asp Arg Asp His Glu Trp Gly Phe Cys Arg His Ser Asn Ser Tyr Arg 
625                 630                 635                 640 


Thr Ser Ser Ile Ile Phe Lys Cys Asp Glu Asp Glu Asp Ile Gly Arg 
                645                 650                 655     


Pro Gln Val Phe Ser Glu Val Arg Gly Cys Asp Val Thr Phe Glu Trp 
            660                 665                 670         


Lys Thr Lys Val Val Cys Pro 
        675                 


<210>  7
<211>  2040
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  His-Biotin-Ci-Mpr Domains 10-13 I1572T.  The underlined sequence 
       change results in the point mutation I1572T and a silent mutation
       S1573 that creates a diagnostic SpeI site.

<400>  7
atgggaatcc caatggggaa gtcgatgctg gtgcttctca ccttcttggc cttcgcctcg     60

tgctgcattg ctgctggcgc gccgaccggt caccatcacc atcaccacgc gccgggcctg    120

aacgacatct tcgaggccca gaagatcgag tggcacgaac ctttcgatct gactgaatgt    180

tcattcaaag atggggctgg caactccttc gacctctcgt ccctgtcaag gtacagtgac    240

aactgggaag ccatcactgg gacgggggac ccggagcact acctcatcaa tgtctgcaag    300

tctctggccc cgcaggctgg cactgagccg tgccctccag aagcagccgc gtgtctgctg    360

ggtggctcca agcccgtgaa cctcggcagg gtaagggacg gacctcagtg gagagatggc    420

ataattgtcc tgaaatacgt tgatggcgac ttatgtccag atgggattcg gaaaaagtca    480

accaccatcc gattcacctg cagcgagagc caagtgaact ccaggcccat gttcatcagc    540

gccgtggagg actgtgagta cacctttgcc tggcccacag ccacagcctg tcccatgaag    600

agcaacgagc atgatgactg ccaggtcacc aacccaagca caggacacct gtttgatctg    660

agctccttaa gtggcagggc gggattcaca gctgcttaca gcgagaaggg gttggtttac    720

atgagcatct gtggggagaa tgaaaactgc cctcctggcg tgggggcctg ctttggacag    780

accaggacta gtgtgggcaa ggccaacaag aggctgagat acgtggacca ggtcctgcag    840

ctggtgtaca aggatgggtc cccttgtccc tccaaatccg gcctgagcta taagagtgtg    900

atcagtttcg tgtgcaggcc tgaggccggg ccaaccaata ggcccatgct catctccctg    960

gacaagcaga catgcactct cttcttctcc tggcacacgc cgctggcctg cgagcaagcg   1020

accgaatgtt ccgtgaggaa tggaagctct attgttgact tgtctcccct tattcatcgc   1080

actggtggtt atgaggctta tgatgagagt gaggatgatg cctccgatac caaccctgat   1140

ttctacatca atatttgtca gccactaaat cccatgcacg gagtgccctg tcctgccgga   1200

gccgctgtgt gcaaagttcc tattgatggt ccccccatag atatcggccg ggtagcagga   1260

ccaccaatac tcaatccaat agcaaatgag atttacttga attttgaaag cagtactcct   1320

tgcttagcgg acaagcattt caactacacc tcgctcatcg cgtttcactg taagagaggt   1380

gtgagcatgg gaacgcctaa gctgttaagg accagcgagt gcgactttgt gttcgaatgg   1440

gagactcctg tcgtctgtcc tgatgaagtg aggatggatg gctgtaccct gacagatgag   1500

cagctcctct acagcttcaa cttgtccagc ctttccacga gcacctttaa ggtgactcgc   1560

gactcgcgca cctacagcgt tggggtgtgc acctttgcag tcgggccaga acaaggaggc   1620

tgtaaggacg gaggagtctg tctgctctca ggcaccaagg gggcatcctt tggacggctg   1680

caatcaatga aactggatta caggcaccag gatgaagcgg tcgttttaag ttacgtgaat   1740

ggtgatcgtt gccctccaga aaccgatgac ggcgtcccct gtgtcttccc cttcatattc   1800

aatgggaaga gctacgagga gtgcatcata gagagcaggg cgaagctgtg gtgtagcaca   1860

actgcggact acgacagaga ccacgagtgg ggcttctgca gacactcaaa cagctaccgg   1920

acatccagca tcatatttaa gtgtgatgaa gatgaggaca ttgggaggcc acaagtcttc   1980

agtgaagtgc gtgggtgtga tgtgacattt gagtggaaaa caaaagttgt ctgcccttga   2040


<210>  8
<211>  679
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-Biotin-Ci-Mpr Domains 10-13 I1572T protein sequence.  The 
       I1572T mutation is underlined.

<400>  8

Met Gly Ile Pro Met Gly Lys Ser Met Leu Val Leu Leu Thr Phe Leu 
1               5                   10                  15      


Ala Phe Ala Ser Cys Cys Ile Ala Ala Gly Ala Pro Thr Gly His His 
            20                  25                  30          


His His His His Ala Pro Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
        35                  40                  45              


Ile Glu Trp His Glu Pro Phe Asp Leu Thr Glu Cys Ser Phe Lys Asp 
    50                  55                  60                  


Gly Ala Gly Asn Ser Phe Asp Leu Ser Ser Leu Ser Arg Tyr Ser Asp 
65                  70                  75                  80  


Asn Trp Glu Ala Ile Thr Gly Thr Gly Asp Pro Glu His Tyr Leu Ile 
                85                  90                  95      


Asn Val Cys Lys Ser Leu Ala Pro Gln Ala Gly Thr Glu Pro Cys Pro 
            100                 105                 110         


Pro Glu Ala Ala Ala Cys Leu Leu Gly Gly Ser Lys Pro Val Asn Leu 
        115                 120                 125             


Gly Arg Val Arg Asp Gly Pro Gln Trp Arg Asp Gly Ile Ile Val Leu 
    130                 135                 140                 


Lys Tyr Val Asp Gly Asp Leu Cys Pro Asp Gly Ile Arg Lys Lys Ser 
145                 150                 155                 160 


Thr Thr Ile Arg Phe Thr Cys Ser Glu Ser Gln Val Asn Ser Arg Pro 
                165                 170                 175     


Met Phe Ile Ser Ala Val Glu Asp Cys Glu Tyr Thr Phe Ala Trp Pro 
            180                 185                 190         


Thr Ala Thr Ala Cys Pro Met Lys Ser Asn Glu His Asp Asp Cys Gln 
        195                 200                 205             


Val Thr Asn Pro Ser Thr Gly His Leu Phe Asp Leu Ser Ser Leu Ser 
    210                 215                 220                 


Gly Arg Ala Gly Phe Thr Ala Ala Tyr Ser Glu Lys Gly Leu Val Tyr 
225                 230                 235                 240 


Met Ser Ile Cys Gly Glu Asn Glu Asn Cys Pro Pro Gly Val Gly Ala 
                245                 250                 255     


Cys Phe Gly Gln Thr Arg Thr Ser Val Gly Lys Ala Asn Lys Arg Leu 
            260                 265                 270         


Arg Tyr Val Asp Gln Val Leu Gln Leu Val Tyr Lys Asp Gly Ser Pro 
        275                 280                 285             


Cys Pro Ser Lys Ser Gly Leu Ser Tyr Lys Ser Val Ile Ser Phe Val 
    290                 295                 300                 


Cys Arg Pro Glu Ala Gly Pro Thr Asn Arg Pro Met Leu Ile Ser Leu 
305                 310                 315                 320 


Asp Lys Gln Thr Cys Thr Leu Phe Phe Ser Trp His Thr Pro Leu Ala 
                325                 330                 335     


Cys Glu Gln Ala Thr Glu Cys Ser Val Arg Asn Gly Ser Ser Ile Val 
            340                 345                 350         


Asp Leu Ser Pro Leu Ile His Arg Thr Gly Gly Tyr Glu Ala Tyr Asp 
        355                 360                 365             


Glu Ser Glu Asp Asp Ala Ser Asp Thr Asn Pro Asp Phe Tyr Ile Asn 
    370                 375                 380                 


Ile Cys Gln Pro Leu Asn Pro Met His Gly Val Pro Cys Pro Ala Gly 
385                 390                 395                 400 


Ala Ala Val Cys Lys Val Pro Ile Asp Gly Pro Pro Ile Asp Ile Gly 
                405                 410                 415     


Arg Val Ala Gly Pro Pro Ile Leu Asn Pro Ile Ala Asn Glu Ile Tyr 
            420                 425                 430         


Leu Asn Phe Glu Ser Ser Thr Pro Cys Leu Ala Asp Lys His Phe Asn 
        435                 440                 445             


Tyr Thr Ser Leu Ile Ala Phe His Cys Lys Arg Gly Val Ser Met Gly 
    450                 455                 460                 


Thr Pro Lys Leu Leu Arg Thr Ser Glu Cys Asp Phe Val Phe Glu Trp 
465                 470                 475                 480 


Glu Thr Pro Val Val Cys Pro Asp Glu Val Arg Met Asp Gly Cys Thr 
                485                 490                 495     


Leu Thr Asp Glu Gln Leu Leu Tyr Ser Phe Asn Leu Ser Ser Leu Ser 
            500                 505                 510         


Thr Ser Thr Phe Lys Val Thr Arg Asp Ser Arg Thr Tyr Ser Val Gly 
        515                 520                 525             


Val Cys Thr Phe Ala Val Gly Pro Glu Gln Gly Gly Cys Lys Asp Gly 
    530                 535                 540                 


Gly Val Cys Leu Leu Ser Gly Thr Lys Gly Ala Ser Phe Gly Arg Leu 
545                 550                 555                 560 


Gln Ser Met Lys Leu Asp Tyr Arg His Gln Asp Glu Ala Val Val Leu 
                565                 570                 575     


Ser Tyr Val Asn Gly Asp Arg Cys Pro Pro Glu Thr Asp Asp Gly Val 
            580                 585                 590         


Pro Cys Val Phe Pro Phe Ile Phe Asn Gly Lys Ser Tyr Glu Glu Cys 
        595                 600                 605             


Ile Ile Glu Ser Arg Ala Lys Leu Trp Cys Ser Thr Thr Ala Asp Tyr 
    610                 615                 620                 


Asp Arg Asp His Glu Trp Gly Phe Cys Arg His Ser Asn Ser Tyr Arg 
625                 630                 635                 640 


Thr Ser Ser Ile Ile Phe Lys Cys Asp Glu Asp Glu Asp Ile Gly Arg 
                645                 650                 655     


Pro Gln Val Phe Ser Glu Val Arg Gly Cys Asp Val Thr Phe Glu Trp 
            660                 665                 670         


Lys Thr Lys Val Val Cys Pro 
        675                 


