                         SEQUENCE LISTING

<110>  Novozymes, Inc.
       Thomas, Michael
       Rey, Michael
 
<120>  Methods of Improving the Introduction of DNA into Bacterial Cells

<130>  10917.204-WO

<150>  60/861,896
<151>  2006-11-29

<160>  19    

<170>  PatentIn version 3.4

<210>  1
<211>  1146
<212>  DNA
<213>  Bacillus licheniformis

<400>  1
atgttctata ctaatcaacc agccatcaac tgcactacat acaaacaaat gctccgctca     60

actggttcgc tatccaattt gttctctgaa agtgactcgc cttatttggt ctcaaggaat    120

gtggaaaatg ctttttgtga agcatttgga gctgaaaact tggggaggtc agactgttct    180

gctgacgctt cattaaatcg tgtcggaatt ggtattaaga cttttcttca tggtaatggt    240

catactcttc aaaaagtagc tgaattcaat aaagactcag acttgtatcg tgggaaatct    300

ccaaaagagc taataaacac ggttgcttct ctccgtaacg agagaattga atttactaaa    360

agaacatatg gtattgattc aatgatatac cactgtgtaa caagaaagcc agggaaaatt    420

cttatttttg aagagccaat ggacttggtt gaaatctcct caattacaaa tgtgaaagta    480

agtaacaaca gaaatacaat cacctttgaa gacggtctac acgaatacag ctttaatgtc    540

actaagagca ccctttataa gcgttttatc actgataaac ctattgaaga aattaatgtt    600

gaaatcttag aaaatcctta tcatgaattg gctaaactat ttggctttga aattccaaaa    660

attccagcac caactgtcaa tccttttgaa aaccttgagc acgttattct tccactcttt    720

tcagaccgtg gctcaaagcg tcatgtacca gaaaaaagcg gtctaaacca atggaatgct    780

ttaggtcgac cacgaaaccc taacgagatt tatataccaa ttccaaaatg gattcataat    840

gtattcccaa catttttccc agctcgtgat aaaccttttc agttacgctt gccagacaaa    900

tcgcttttat cagccaaggt atgccaagac aatagtaaag cacttatgtc taatccaaat    960

agtgctcttg gagaatggct actaagacaa gttatgaact tagaggaaaa agaacttcta   1020

acctatgaaa tgctggaaag actaaatatt gactcagtaa ttgtttataa acacagcgaa   1080

caacattact ccattgattt ttgtgaaatg ggttcttatg atgaatttga aaatgaaaac   1140

aaataa                                                              1146


<210>  2
<211>  381
<212>  PRT
<213>  Bacillus licheniformis

<400>  2

Met Phe Tyr Thr Asn Gln Pro Ala Ile Asn Cys Thr Thr Tyr Lys Gln 
1               5                   10                  15      


Met Leu Arg Ser Thr Gly Ser Leu Ser Asn Leu Phe Ser Glu Ser Asp 
            20                  25                  30          


Ser Pro Tyr Leu Val Ser Arg Asn Val Glu Asn Ala Phe Cys Glu Ala 
        35                  40                  45              


Phe Gly Ala Glu Asn Leu Gly Arg Ser Asp Cys Ser Ala Asp Ala Ser 
    50                  55                  60                  


Leu Asn Arg Val Gly Ile Gly Ile Lys Thr Phe Leu His Gly Asn Gly 
65                  70                  75                  80  


His Thr Leu Gln Lys Val Ala Glu Phe Asn Lys Asp Ser Asp Leu Tyr 
                85                  90                  95      


Arg Gly Lys Ser Pro Lys Glu Leu Ile Asn Thr Val Ala Ser Leu Arg 
            100                 105                 110         


Asn Glu Arg Ile Glu Phe Thr Lys Arg Thr Tyr Gly Ile Asp Ser Met 
        115                 120                 125             


Ile Tyr His Cys Val Thr Arg Lys Pro Gly Lys Ile Leu Ile Phe Glu 
    130                 135                 140                 


Glu Pro Met Asp Leu Val Glu Ile Ser Ser Ile Thr Asn Val Lys Val 
145                 150                 155                 160 


Ser Asn Asn Arg Asn Thr Ile Thr Phe Glu Asp Gly Leu His Glu Tyr 
                165                 170                 175     


Ser Phe Asn Val Thr Lys Ser Thr Leu Tyr Lys Arg Phe Ile Thr Asp 
            180                 185                 190         


Lys Pro Ile Glu Glu Ile Asn Val Glu Ile Leu Glu Asn Pro Tyr His 
        195                 200                 205             


Glu Leu Ala Lys Leu Phe Gly Phe Glu Ile Pro Lys Ile Pro Ala Pro 
    210                 215                 220                 


Thr Val Asn Pro Phe Glu Asn Leu Glu His Val Ile Leu Pro Leu Phe 
225                 230                 235                 240 


Ser Asp Arg Gly Ser Lys Arg His Val Pro Glu Lys Ser Gly Leu Asn 
                245                 250                 255     


Gln Trp Asn Ala Leu Gly Arg Pro Arg Asn Pro Asn Glu Ile Tyr Ile 
            260                 265                 270         


Pro Ile Pro Lys Trp Ile His Asn Val Phe Pro Thr Phe Phe Pro Ala 
        275                 280                 285             


Arg Asp Lys Pro Phe Gln Leu Arg Leu Pro Asp Lys Ser Leu Leu Ser 
    290                 295                 300                 


Ala Lys Val Cys Gln Asp Asn Ser Lys Ala Leu Met Ser Asn Pro Asn 
305                 310                 315                 320 


Ser Ala Leu Gly Glu Trp Leu Leu Arg Gln Val Met Asn Leu Glu Glu 
                325                 330                 335     


Lys Glu Leu Leu Thr Tyr Glu Met Leu Glu Arg Leu Asn Ile Asp Ser 
            340                 345                 350         


Val Ile Val Tyr Lys His Ser Glu Gln His Tyr Ser Ile Asp Phe Cys 
        355                 360                 365             


Glu Met Gly Ser Tyr Asp Glu Phe Glu Asn Glu Asn Lys 
    370                 375                 380     


<210>  3
<211>  1014
<212>  DNA
<213>  Bacillus licheniformis

<400>  3
ttgacttatc gagtaggtag tatgtttgct gggataggtg gaacttgttt agggtttatc     60

caagctggcg ctaggattgt ctgggcaaat gaaatagaca aaaatgcttg tattacttat    120

agaaattatt ttggggatgc ttacttacaa gagggtgaca ttaacctaat agataaaaac    180

tccatacctg aactggacat tttgattgga ggttttcctt gccaagcctt ctctatagct    240

ggctatcgta aagggtttga agatgaaagg ggaaacgtgt tctttcaaat attagaggta    300

ttggaagcac aaagaaatgt ttatggacac ttaccccaag caataatgct tgagaatgta    360

aagaacttat ttacacatga tagaggtaat acgtacagag taataaaaga ggctttggaa    420

gcctttggtt ataccgtaaa agctgaggtt cttaattcaa tggaatacgg taacgtgcca    480

caaaacagag agcggattta tattgtaggt tttcaagatg agagccaagc tgaaaggttt    540

agctttccag acccaattcc tttaacaaat caacttaatg atgtaattga ccgaactcgg    600

agagttgata aaagatatta ttatgatgaa acctctcaat attatgatat gttgcgagaa    660

gccatggaca gtacagatac aacttatcaa ataagacgta tatatgttcg agaaaataga    720

agcaatgttt gtcctacact gacagcgaat atgggaactg gagggcataa tgttcctatt    780

gtattagact ttgaaaataa tataagaaaa ctaacaccag aagaatgctt actattgcaa    840

ggtttcccag ctgactatca ttttccagaa ggcatggcaa acactcacaa atataaacaa    900

gctggtaact ctgttacggt gccagttata agaagaattg ccactaatat tattagcgta    960

ttgaacattg gaatgaatat aaatcaagaa catgaatatg caatagctga ataa         1014


<210>  4
<211>  337
<212>  PRT
<213>  Bacillus licheniformis

<400>  4

Met Thr Tyr Arg Val Gly Ser Met Phe Ala Gly Ile Gly Gly Thr Cys 
1               5                   10                  15      


Leu Gly Phe Ile Gln Ala Gly Ala Arg Ile Val Trp Ala Asn Glu Ile 
            20                  25                  30          


Asp Lys Asn Ala Cys Ile Thr Tyr Arg Asn Tyr Phe Gly Asp Ala Tyr 
        35                  40                  45              


Leu Gln Glu Gly Asp Ile Asn Leu Ile Asp Lys Asn Ser Ile Pro Glu 
    50                  55                  60                  


Leu Asp Ile Leu Ile Gly Gly Phe Pro Cys Gln Ala Phe Ser Ile Ala 
65                  70                  75                  80  


Gly Tyr Arg Lys Gly Phe Glu Asp Glu Arg Gly Asn Val Phe Phe Gln 
                85                  90                  95      


Ile Leu Glu Val Leu Glu Ala Gln Arg Asn Val Tyr Gly His Leu Pro 
            100                 105                 110         


Gln Ala Ile Met Leu Glu Asn Val Lys Asn Leu Phe Thr His Asp Arg 
        115                 120                 125             


Gly Asn Thr Tyr Arg Val Ile Lys Glu Ala Leu Glu Ala Phe Gly Tyr 
    130                 135                 140                 


Thr Val Lys Ala Glu Val Leu Asn Ser Met Glu Tyr Gly Asn Val Pro 
145                 150                 155                 160 


Gln Asn Arg Glu Arg Ile Tyr Ile Val Gly Phe Gln Asp Glu Ser Gln 
                165                 170                 175     


Ala Glu Arg Phe Ser Phe Pro Asp Pro Ile Pro Leu Thr Asn Gln Leu 
            180                 185                 190         


Asn Asp Val Ile Asp Arg Thr Arg Arg Val Asp Lys Arg Tyr Tyr Tyr 
        195                 200                 205             


Asp Glu Thr Ser Gln Tyr Tyr Asp Met Leu Arg Glu Ala Met Asp Ser 
    210                 215                 220                 


Thr Asp Thr Thr Tyr Gln Ile Arg Arg Ile Tyr Val Arg Glu Asn Arg 
225                 230                 235                 240 


Ser Asn Val Cys Pro Thr Leu Thr Ala Asn Met Gly Thr Gly Gly His 
                245                 250                 255     


Asn Val Pro Ile Val Leu Asp Phe Glu Asn Asn Ile Arg Lys Leu Thr 
            260                 265                 270         


Pro Glu Glu Cys Leu Leu Leu Gln Gly Phe Pro Ala Asp Tyr His Phe 
        275                 280                 285             


Pro Glu Gly Met Ala Asn Thr His Lys Tyr Lys Gln Ala Gly Asn Ser 
    290                 295                 300                 


Val Thr Val Pro Val Ile Arg Arg Ile Ala Thr Asn Ile Ile Ser Val 
305                 310                 315                 320 


Leu Asn Ile Gly Met Asn Ile Asn Gln Glu His Glu Tyr Ala Ile Ala 
                325                 330                 335     


Glu 
    


<210>  5
<211>  3049
<212>  DNA
<213>  Bacillus licheniformis

<400>  5
tcatgttccc atattctttt aatgttccaa tccttttcct cgtaatattt attaacttcc     60

ttatctcttt ttttatttct ttcgagtttt ttctcccaat attccgtatt actttttggt    120

atattcccgt gtttttcaca cgcatgccag aaacaagaat caatgaatat gactatttta    180

tatttctgta ttactatatc tggactaccg tataatttct taacattttt tcggaatctt    240

attccacggt gccatagttc tttagtaacc ttatcttcta attttgaacg agatttgatt    300

gcctgcatgt tttttcttct ttgttctttt gaaaccgtgt cagtcataga agagtcctcc    360

aaagccacaa taattgtatt ctataaacga ggaagcaagc cctcaagctt accccctctt    420

agttcctttt ttgcctactt atttatttgt tttcattttc aaattcatca taagaaccca    480

tttcacaaaa atcaatggag taatgttgtt cgctgtgttt ataaacaatt actgagtcaa    540

tatttagtct ttccagcatt tcataggtta gaagttcttt ttcctctaag ttcataactt    600

gtcttagtag ccattctcca agagcactat ttggattaga cataagtgct ttactattgt    660

cttggcatac cttggctgat aaaagcgatt tgtctggcaa gcgtaactga aaaggtttat    720

cacgagctgg gaaaaatgtt gggaatacat tatgaatcca ttttggaatt ggtatataaa    780

tctcgttagg gtttcgtggt cgacctaaag cattccattg gtttagaccg cttttttctg    840

gtacatgacg ctttgagcca cggtctgaaa agagtggaag aataacgtgc tcaaggtttt    900

caaaaggatt gacagttggt gctggaattt ttggaatttc aaagccaaat agtttagcca    960

attcatgata aggattttct aagatttcaa cattaatttc ttcaataggt ttatcagtga   1020

taaaacgctt ataaagggtg ctcttagtga cattaaagct gtattcgtgt agaccgtctt   1080

caaaggtgat tgtatttctg ttgttactta ctttcacatt tgtaattgag gagatttcaa   1140

ccaagtccat tggctcttca aaaataagaa ttttccctgg ctttcttgtt acacagtggt   1200

atatcattga atcaatacca tatgttcttt tagtaaattc aattctctcg ttacggagag   1260

aagcaaccgt gtttattagc tcttttggag atttcccacg atacaagtct gagtctttat   1320

tgaattcagc tactttttga agagtatgac cattaccatg aagaaaagtc ttaataccaa   1380

ttccgacacg atttaatgaa gcgtcagcag aacagtctga cctccccaag ttttcagctc   1440

caaatgcttc acaaaaagca ttttccacat tccttgagac caaataaggc gagtcacttt   1500

cagagaacaa attggatagc gaaccagttg agcggagcat ttgtttgtat gtagtgcagt   1560

tgatggctgg ttgattagta tagaacatta tttttcctcc tcttttatgc ttgtcatttc   1620

ttctttcaga cccaaaaggt agtcagctga tacgttcaat gtttcagcta ttcttttgaa   1680

agtgtccaat gatggagttc tattttcact ttcatatagt gaccaagtgc ttctagtgac   1740

cccgactttt tcagcgattt ggctgggtaa taacctacga gcttctcttg cattttgaat   1800

acgatttcca aggaaaggta tcatttttgc acctccaaga tttgttgttt tcagagtatc   1860

accagaaccc ccgaaaatag tccaaagtta gctaacagca aacaaataaa aataaataag   1920

ttgtttactc ttagcaaact tgttactaaa atttgataaa gttattcatt taatccagct   1980

cttatgctaa aattgcatta gcggacaagc ttaatgtttg caaggaggta taattttgac   2040

ttatcgagta ggtagtatgt ttgctgggat aggtggaact tgtttagggt ttatccaagc   2100

tggcgctagg attgtctggg caaatgaaat agacaaaaat gcttgtatta cttatagaaa   2160

ttattttggg gatgcttact tacaagaggg tgacattaac ctaatagata aaaactccat   2220

acctgaactg gacattttga ttggaggttt tccttgccaa gccttctcta tagctggcta   2280

tcgtaaaggg tttgaagatg aaaggggaaa cgtgttcttt caaatattag aggtattgga   2340

agcacaaaga aatgtttatg gacacttacc ccaagcaata atgcttgaga atgtaaagaa   2400

cttatttaca catgatagag gtaatacgta cagagtaata aaagaggctt tggaagcctt   2460

tggttatacc gtaaaagctg aggttcttaa ttcaatggaa tacggtaacg tgccacaaaa   2520

cagagagcgg atttatattg taggttttca agatgagagc caagctgaaa ggtttagctt   2580

tccagaccca attcctttaa caaatcaact taatgatgta attgaccgaa ctcggagagt   2640

tgataaaaga tattattatg atgaaacctc tcaatattat gatatgttgc gagaagccat   2700

ggacagtaca gatacaactt atcaaataag acgtatatat gttcgagaaa atagaagcaa   2760

tgtttgtcct acactgacag cgaatatggg aactggaggg cataatgttc ctattgtatt   2820

agactttgaa aataatataa gaaaactaac accagaagaa tgcttactat tgcaaggttt   2880

cccagctgac tatcattttc cagaaggcat ggcaaacact cacaaatata aacaagctgg   2940

taactctgtt acggtgccag ttataagaag aattgccact aatattatta gcgtattgaa   3000

cattggaatg aatataaatc aagaacatga atatgcaata gctgaataa               3049


<210>  6
<211>  26
<212>  DNA
<213>  Bacillus licheniformis

<400>  6
gagctctgca aggaggtata attttg                                          26


<210>  7
<211>  27
<212>  DNA
<213>  Bacillus licheniformis

<400>  7
acgcgtttat tcagctattg catattc                                         27


<210>  8
<211>  37
<212>  DNA
<213>  Bacillus licheniformis

<400>  8
ccaggcctta agggccgcat gcgtccttct ttgtgct                              37


<210>  9
<211>  27
<212>  DNA
<213>  Bacillus licheniformis

<400>  9
gagctccttt caatgtgata catatga                                         27


<210>  10
<211>  24
<212>  DNA
<213>  Bacillus licheniformis

<400>  10
gcggccgctc gctttccaat ctga                                            24


<210>  11
<211>  24
<212>  DNA
<213>  Bacillus licheniformis

<400>  11
atcgatcagc ttggataaac ccta                                            24


<210>  12
<211>  26
<212>  DNA
<213>  Bacillus licheniformis

<400>  12
gagctctgca aggaggtata attttg                                          26


<210>  13
<211>  28
<212>  DNA
<213>  Bacillus licheniformis

<400>  13
cgtcgacgcc tttgcggtag tggtgctt                                        28


<210>  14
<211>  26
<212>  DNA
<213>  Bacillus licheniformis

<400>  14
ggtaccatgt tctatactaa tcaacc                                          26


<210>  15
<211>  26
<212>  DNA
<213>  Bacillus licheniformis

<400>  15
ggatccttat ttgttttcat tttcaa                                          26


<210>  16
<211>  24
<212>  DNA
<213>  Bacillus licheniformis

<400>  16
atcgatcagc ttggataaac ccta                                            24


<210>  17
<211>  30
<212>  DNA
<213>  Bacillus licheniformis

<400>  17
ttcacaagat ctatttcttc tttcagaccc                                      30


<210>  18
<211>  30
<212>  DNA
<213>  Bacillus licheniformis

<400>  18
agaaatagat cttgtgaaat gggttcttat                                      30


<210>  19
<211>  26
<212>  DNA
<213>  Bacillus licheniformis

<400>  19
gcggccgctc atgttcccat attctt                                          26


