                              Sequence Listing

<110> Genentech, Inc.
      Davis, David P.
      Gray, Daniel C.
      Gu, Zhenyu

<120> COMPOSITIONS AND METHODS FOR THE EXPRESSION OF 
      NUCLEIC ACIDS

<130> P2440R1

<141> 2007-12-04

<150> US 60/871,390
<151> 2006-12-21

<160> 28

<210> 1
<211> 13
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 1
 ccctagaaag  ata 13

<210> 2
<211> 35
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 2
 ccctagaaag atagtctgcg taaaattgac  gcatg 35

<210> 3
<211> 63
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 3
 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta 50

 aaattgacgc  atg 63

<210> 4
<211> 63
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 4
 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat 50

 tatctttcta  ggg 63

<210> 5
<211> 35
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 5
 catgcgtcaa ttttacgcag actatctttc  taggg 35

<210> 6
<211> 29
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 6
 gttcaagtcg gaagtttaca  tacacttag 29

<210> 7
<211> 30
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 7
 cagtgggtca gaagtttaca tacactaagg 30

<210> 8
<211> 31
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 8
 cagtgggtca gaagttaaca tacactcaat  t 31

<210> 9
<211> 30
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 9
 agttgaatcg gaagtttaca tacaccttag 30

<210> 10
<211> 226
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 10
 agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg 50

 tttttcaact acaccacaaa tttcttgtta acaaacaata gttttggcaa 100

 gtcagttagg acatctactt tgtgcatgac acaagtcatt tttccaacaa 150

 ttgtttacag acagattatt tcacttataa ttcactgtat cacaattcca 200

 gtgggtcaga agtttacata  cactaa 226

<210> 11
<211> 228
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 11
 ttgagtgtat gttaacttct gacccactgg gaatgtgatg aaagaaataa 50

 aagctgaaat gaatcattct ctctactatt attctgatat ttcacattct 100

 taaaataaag tggtgatcct aactgacctt aagacaggga atctttactc 150

 ggattaaatg tcaggaattg tgaaaaagtg agtttaatgt atttggctaa 200

 ggtgtatgta aacttccgac  ttcaactg 228

<210> 12
<211> 21
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 12
 tgtgaaaaag tgtttgcccc  c 21

<210> 13
<211> 250
<212> DNA
<213> Artificial sequence

<220>
<223> inverted repeat

<400> 13
 cagtggtgtg aaaaagtgtt tgcccccttc ctcatttcct gttcctttgc 50

 atgtttgtca cacttaagtg tttcggaaca tcaaaccaat ttaaacaata 100

 gtcaaggaca acacaagtaa acacaaaatg caatttgtaa atgaaggtgt 150

 ttattattaa aggtgaaaaa aaatccaaac catcatggcc ctgtgtgaaa 200

 aagtgattgc cccccttgtt aaaacatact ataactgtgg ttgtccacac 250

<210> 14
<211> 594
<212> PRT
<213> Trichopulsia ni

<400> 14
 Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu
   1               5                  10                  15

 Gln Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile
                  20                  25                  30

 Ser Asp His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu
                  35                  40                  45

 Ala Phe Ile Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly
                  50                  55                  60

 Ser Glu Ile Leu Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser
                  65                  70                  75

 Ser Leu Ala Ser Asn Lys Ile Leu Thr Leu Pro Gln Arg Thr Ile
                  80                  85                  90

 Arg Gly Lys Asn Lys His Cys Trp Ser Thr Ser Lys Ser Thr Arg
                  95                 100                 105

 Arg Ser Arg Val Ser Ala Leu Asn Ile Val Arg Ser Gln Arg Gly
                 110                 115                 120

 Pro Thr Arg Met Cys Arg Asn Ile Tyr Asp Pro Leu Leu Cys Phe
                 125                 130                 135

 Lys Leu Phe Phe Thr Asp Glu Ile Ile Ser Glu Ile Val Lys Trp
                 140                 145                 150

 Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg Glu Ser Met Thr Gly
                 155                 160                 165

 Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile Tyr Ala Phe Phe
                 170                 175                 180

 Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn His Met Ser
                 185                 190                 195

 Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr Val Ser
                 200                 205                 210

 Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu Arg
                 215                 220                 225

 Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val
                 230                 235                 240

 Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys
                 245                 250                 255

 Ile Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln
                 260                 265                 270

 Leu Leu Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro
                 275                 280                 285

 Asn Lys Pro Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp
                 290                 295                 300

 Ser Gly Thr Lys Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg
                 305                 310                 315

 Gly Thr Gln Thr Asn Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys
                 320                 325                 330

 Glu Leu Ser Lys Pro Val Arg Gly Ser Cys Arg Asn Ile Thr Cys
                 335                 340                 345

 Asp Asn Trp Phe Thr Ser Ile Pro Leu Ala Lys Asn Leu Leu Gln
                 350                 355                 360

 Glu Pro Tyr Lys Leu Thr Ile Val Gly Thr Val Arg Ser Asn Lys
                 365                 370                 375

 Arg Glu Ile Pro Glu Val Leu Lys Asn Ser Arg Ser Arg Pro Val
                 380                 385                 390

 Gly Thr Ser Met Phe Cys Phe Asp Gly Pro Leu Thr Leu Val Ser
                 395                 400                 405

 Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu Leu Ser Ser Cys
                 410                 415                 420

 Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys Pro Gln Met
                 425                 430                 435

 Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr Leu Asp
                 440                 445                 450

 Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg Trp
                 455                 460                 465

 Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn
                 470                 475                 480

 Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys
                 485                 490                 495

 Val Gln Ser Arg Glu Lys Phe Met Arg Asn Leu Tyr Met Ser Leu
                 500                 505                 510

 Thr Ser Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys
                 515                 520                 525

 Arg Tyr Leu Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val
                 530                 535                 540

 Pro Gly Thr Ser Asp Asp Ser Thr Glu Glu Pro Val Thr Lys Lys
                 545                 550                 555

 Arg Thr Tyr Cys Thr Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala
                 560                 565                 570

 Asn Ala Ser Cys Lys Lys Cys Lys Lys Val Ile Cys Arg Glu His
                 575                 580                 585

 Asn Ile Asp Met Cys Gln Ser Cys Phe
                 590                

<210> 15
<211> 656
<212> DNA
<213> Artificial sequence

<220>
<223> codon-optimized TetR

<400> 15
 atgtccagac tggataagtc caaggtgatt aattccgctc tggaactcct 50

 gaacgaggtc ggcatcgagg gactgaccac acggaagctg gctcagaaac 100

 tcggcgtcga acagcctacc ctctactggc atgtcaaaaa taagagagcc 150

 ctcctggacg ccctggctat cgagatgctg gacagacacc acacccactt 200

 ctgccccctg gaaggcgaat cctggcagga tttcctccgg aacaacgcta 250

 aaagctttag atgcgccctc ctcagccata gagacggagc taaagtgcac 300

 ctgggaaccc ggcctacaga aaaacagtac gagacactgg aaaaccagct 350

 cgctttcctc tgccaacaag gctttagcct ggaaaacgcc ctctacgctc 400

 tcagcgctgt cggccatttt acactgggct gcgtgctcga ggaccaggag 450

 caccaagtgg ctaaagagag cgggaaaccc ctaccaccga tagcatgccc 500

 cccctgctga gacaagccat tgagctcttt gatcatcagg gagctgaacc 550

 cgccttcctc tttggactcg aactcattat ttgcggactc gagaagcaac 600

 tgaaatgcga aagcggaagc gcctactccg gctccagaga atttcggtcc 650

 tactag 656

<210> 16
<211> 225
<212> DNA
<213> Artificial sequence

<220>
<223> H1-tetO2-2X promoter

<400> 16
 cgaacgctga cgtcatcaac ccgctccaag gaatcgcggg cccagtgtca 50

 ctaggcggga acacccagcg cgcgtgcgcc ctggcaggaa gatggctgtg 100

 agggacaggg gagtggcgcc ctgcaatatt tgcatgtcgc tatgtgttct 150

 gggaaatcac cataaacgtg aaatccctat cagtgataga gacttataag 200

 ttccctatca gtgatagaga  tcccc 225

<210> 17
<211> 9611
<212> DNA
<213> Artificial sequence

<220>
<223> PB(luc-shRNA) construct

<400> 17
 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 50

 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 100

 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 150

 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 200

 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 250

 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300

 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 350

 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 400

 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 450

 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 500

 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 550

 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600

 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 650

 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 700

 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 750

 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 800

 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 850

 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900

 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 950

 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1000

 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 1050

 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 1100

 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1150

 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200

 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 1250

 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1300

 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 1350

 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1400

 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1450

 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500

 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 1550

 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1600

 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 1650

 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 1700

 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 1750

 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800

 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 1850

 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 1900

 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 1950

 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 2000

 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 2050

 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2100

 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 2150

 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct 2200

 gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg 2250

 aattcaatta gaaactatta tttaacccta gaaagataat catattgtga 2300

 cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt 2350

 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta 2400

 tatttacact tacatactaa taataaattc aacaaactat ttatttatgt 2450

 ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt 2500

 aacaaaactt ttaaacattc tctcttttac aaaaataaac ttattttgta 2550

 ctttaaaaac agtcatgttg tattataaaa taagtaatta gcttaactta 2600

 tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg 2650

 cacatatcaa tattatgctc tcgacaaata acttttttgc attttttgca 2700

 cgatgcattt gcctttcgcc ttattttaga ggggcagtaa gtacagtaag 2750

 tacgtttttt cgttactggc tcttcagtac tgtcatctga tgtaccaggc 2800

 acttcatttg gcaaaatatt agagatatta tcgcgcaaat atctcttcaa 2850

 agtaggagct tctaaacgct tacgcataaa cgatgacgtc aggctcatgt 2900

 aaaggtttct cataaatttt ttgcgacttt gaaccttttc tcccttgcta 2950

 ctgacattat ggctgtatat aataaaagaa tttatgcagg caatgtttat 3000

 cattccgtac aataatgcca taggccacct attcgtcttc ctactgcagg 3050

 tcatcacaga acacatttgg tctagcgtgt ccactccgcc tttagtttga 3100

 ttataataca taaccatttg cggtttaccg gtactttcgt tgatagaagc 3150

 atcctcatca caagatgata ataagtatac catcttagct ggcttcggtt 3200

 tatatgagac gagagtaagg ggtccgtcaa aacaaaacat cgatgttccc 3250

 actggcctgg agcgactgtt tttcagtact tccggtatct cgcgtttgtt 3300

 tgatcgcacg gttcccacaa tggttaaccg aacgctgacg tcatcaaccc 3350

 gctccaagga atcgcgggcc cagtgtcact aggcgggaac acccagcgcg 3400

 cgtgcgccct ggcaggaaga tggctgtgag ggacagggga gtggcgccct 3450

 gcaatatttg catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa 3500

 atccctatca gtgatagaga cttataagtt ccctatcagt gatagagatc 3550

 ccccgatatg ggctgaatac aattcaagag attgtattca gcccatatcg 3600

 ttttttggaa aagcttatcg ataccgtcga cctcgagggg gggcccggta 3650

 cccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc 3700

 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 3750

 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 3800

 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 3850

 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 3900

 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 3950

 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 4000

 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 4050

 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 4100

 gcgtttttcc ataggctcca ctagtaaagg gaaaaccggt gttccatgtc 4150

 cttatatgga ctcatctttg cctattgcga cacacactca atgaacacct 4200

 actacgcgct gcaaagagcc ccgcaggcct gaggtgcccc cacctcacca 4250

 ctcttcctat ttttgtgtaa aaatccagct tcttgtcacc acctccaagg 4300

 agggggagga ggaggaaggc aggttcctct aggctgagcc gaatgcccct 4350

 ctgtggtccc acgccactga tcgctgcatg cccaccacct gggtacacac 4400

 agtctgtgat tcccggagca gaacggaccc tgcccacccg gtcttgtgtg 4450

 ctactcagtg gacagaccca aggcaagaaa gggtgacaag gacagggtct 4500

 tcccaggctg gctttgagtt cctagcaccg ccccgccccc aatcctctgt 4550

 ggcacatgga gtcttggtcc ccagagtccc ccagcggcct ccagatggtc 4600

 tgggagggca gttcagctgt ggctgcgcat agcagacata caacggacgg 4650

 tgggcccaga cccaggctgt gtagacccag cccccccgcc ccgcagtgcc 4700

 taggtcaccc actaacgccc caggcctggt cttggctggg cgtgactgtt 4750

 accctcaaaa gcaggcagct ccagggtaaa aggtgccctg ccctgtagag 4800

 cccaccttcc ttcccagggc tgcggctggg taggtttgta gccttcatca 4850

 cgggccacct ccagccactg gaccgctggc ccctgccctg tcctggggag 4900

 tgtggtcctg cgacttctaa gtggccgcaa gccacctgac tcccccaaca 4950

 ccacactcta cctctcaagc ccaggtctct ccctagtgac ccacccagca 5000

 catttagcta gctgagcccc acagccagag gtcctcaggc cctgctttca 5050

 gggcagttgc tctgaagtcg gcaaggggga gtgactgcct ggccactcca 5100

 tgccctccaa gagctccttc tgcaggagcg tacagaaccc agggccctgg 5150

 cacccgtgca gaccctggcc caccccacct gggcgctcag tgcccaagag 5200

 atgtccacac ctaggatgtc ccgcggtggg tggggggccc gagagacggg 5250

 caggccgggg gcaggcctgg ccatgcgggg ccgaaccggg cactgcccag 5300

 cgtggggcgc gggggccacg gcgcgcgccc ccagcccccg ggcccagcac 5350

 cccaaggcgg ccaacgccaa aactctccct cctcctcttc ctcaatctcg 5400

 ctctcgctct tttttttttt cgcaaaagga ggggagaggg ggtaaaaaaa 5450

 tgctgcactg tgcggcgaag ccggtgagtg agcggcgcgg ggccaatcag 5500

 cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg ccgcggcggc 5550

 gccctataaa acccagcggc gcgacgcgcc accaccgccg agaccgcgtc 5600

 cgccccgcga gcacagagcc tcgcctttgc cgatccgccg cccgtccaag 5650

 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg 5700

 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg 5750

 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 5800

 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct 5850

 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt 5900

 tttctgttct gcgccgttac agatccaagc caccatgtcc agactggata 5950

 agtccaaggt gattaattcc gctctggaac tcctgaacga ggtcggcatc 6000

 gagggactga ccacacggaa gctggctcag aaactcggcg tcgaacagcc 6050

 taccctctac tggcatgtca aaaataagag agccctcctg gacgccctgg 6100

 ctatcgagat gctggacaga caccacaccc acttctgccc cctggaaggc 6150

 gaatcctggc aggatttcct ccggaacaac gctaaaagct ttagatgcgc 6200

 cctcctcagc catagagacg gagctaaagt gcacctggga acccggccta 6250

 cagaaaaaca gtacgagaca ctggaaaacc agctcgcttt cctctgccaa 6300

 caaggcttta gcctggaaaa cgccctctac gctctcagcg ctgtcggcca 6350

 ttttacactg ggctgcgtgc tcgaggacca ggagcaccaa gtggctaaag 6400

 aggagcggga aacccctacc accgatagca tgccccccct gctgagacaa 6450

 gccattgagc tctttgatca tcagggagct gaacccgcct tcctctttgg 6500

 actcgaactc attatttgcg gactcgagaa gcaactgaaa tgcgaaagcg 6550

 gaagcgccta ctccggctcc agagaatttc ggtcctacta ggaattccgc 6600

 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata 6650

 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt 6700

 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt 6750

 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt 6800

 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 6850

 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc 6900

 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 6950

 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct 7000

 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 7050

 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta 7100

 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt 7150

 cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc 7200

 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 7250

 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 7300

 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct 7350

 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 7400

 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 7450

 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 7500

 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 7550

 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 7600

 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 7650

 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 7700

 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 7750

 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 7800

 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac 7850

 cccatggctc cgaccgaagc cgacccgggc ggccccgccg accccgcacc 7900

 cgcccccgag gcccaccgac tctagagctc gctgatcagc ctcgactgtg 7950

 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 8000

 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 8050

 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 8100

 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 8150

 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 8200

 cgaggtgcat tctagttgtg ggatctgaca atgttcagtg cagagactcg 8250

 gctacgcctc gtggactttg aagttgacca acaatgttta ttcttacctc 8300

 taatagtcct ctgtggcaag gtcaagattt tgttagaagc caatgaagaa 8350

 cctggttgtt caataacatt ttgttcgtct aatatttcac taccgcttga 8400

 cgttggctgc acttcatgta cctcatctat aaacgcttct tctgtatcgc 8450

 tctggacgtc atcttcactt acgtgatctg atatttcact gtcagaatcc 8500

 tcaccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc 8550

 atcgtctaaa gaactaccca ttttattata tattagtcac gatatctata 8600

 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata 8650

 acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa 8700

 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca 8750

 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag 8800

 atgtcctaaa cgcacagcga cggattcgcg ctatttagaa agagagagca 8850

 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt 8900

 taaaaaagat ttgcgctgga tccactagtt ctagagcggc cgccaccgcg 8950

 gtggagctcc aattcgccct atagtgagtc gtattacgcg cgctcactgg 9000

 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 9050

 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 9100

 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 9150

 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 9200

 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 9250

 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 9300

 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 9350

 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 9400

 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 9450

 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 9500

 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 9550

 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 9600

 ttacaattta  g 9611

<210> 18
<211> 4965
<212> DNA
<213> Artificial sequence

<220>
<223> pCAG-PBase

<220>
<221> Unsure
<222> 4956-4965
<223> Unknown base

<400> 18
 cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 50

 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 100

 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 150

 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 200

 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 250

 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 300

 gctattaaca tggtcgaggt gagccccacg ttctgcttca ctctccccat 350

 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat 400

 tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccaggcggg 450

 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc 500

 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc 550

 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc ggggagtcgc 600

 tgcgacgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc 650

 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga 700

 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg 750

 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct 800

 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg 850

 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc 900

 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc 950

 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg 1000

 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg 1050

 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc 1100

 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt 1150

 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc 1200

 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc 1250

 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc 1300

 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 1350

 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg 1400

 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc 1450

 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 1500

 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt 1550

 gtgaccggcg gctctagaca attgtactaa ccttcttctc tttcctctcc 1600

 tgacaggttg gtgtacagta gcttccacca tgggtagttc tttagacgat 1650

 gagcatatcc tctctgctct tctgcaaagc gatgacgagc ttgttggtga 1700

 ggattctgac agtgaaatat cagatcacgt aagtgaagat gacgtccaga 1750

 gcgatacaga agaagcgttt atagatgagg tacatgaagt gcagccaacg 1800

 tcaagcggta gtgaaatatt agacgaacaa aatgttattg aacaaccagg 1850

 ttcttcattg gcttctaaca aaatcttgac cttgccacag aggactatta 1900

 gaggtaagaa taaacattgt tggtcaactt caaagtccac gaggcgtagc 1950

 cgagtctctg cactgaacat tgtcagatct caaagaggtc cgacgcgtat 2000

 gtgccgcaat atatatgacc cacttttatg cttcaaacta ttttttactg 2050

 atgagataat ttcggaaatt gtaaaatgga caaatgctga gatatcattg 2100

 aaacgtcggg aatctatgac aggtgctaca tttcgtgaca cgaatgaaga 2150

 tgaaatctat gctttctttg gtattctggt aatgacagca gtgagaaaag 2200

 ataaccacat gtccacagat gacctctttg atcgatcttt gtcaatggtg 2250

 tacgtctctg taatgagtcg tgatcgtttt gattttttga tacgatgtct 2300

 tagaatggat gacaaaagta tacggcccac acttcgagaa aacgatgtat 2350

 ttactcctgt tagaaaaata tgggatctct ttatccatca gtgcatacaa 2400

 aattacactc caggggctca tttgaccata gatgaacagt tacttggttt 2450

 tagaggacgg tgtccgttta ggatgtatat cccaaacaag ccaagtaagt 2500

 atggaataaa aatcctcatg atgtgtgaca gtggtacaaa gtatatgata 2550

 aatggaatgc cttatttggg aagaggaaca cagaccaacg gagtaccact 2600

 cggtgaatac tacgtgaagg agttatcaaa gcctgtgcac ggtagttgtc 2650

 gtaatattac gtgtgacaat tggttcacct caatcccttt ggcaaaaaac 2700

 ttactacaag aaccgtataa gttaaccatt gtgggaaccg tgcgatcaaa 2750

 caaacgcgag ataccggaag tactgaaaaa cagtcgctcc aggccagtgg 2800

 gaacatcgat gttttgtttt gacggacccc ttactctcgt ctcatataaa 2850

 ccgaagccag ctaagatggt atacttatta tcatcttgtg atgaggatgc 2900

 ttctatcaac gaaagtaccg gtaaaccgca aatggttatg tattataatc 2950

 aaactaaagg cggagtggac acgctagacc aaatgtgttc tgtgatgacc 3000

 tgcagtagga agacgaatag gtggcctatg gcattattgt acggaatgat 3050

 aaacattgcc tgcataaatt cttttattat atacagccat aatgtcagta 3100

 gcaagggaga aaaggttcaa agtcgcaaaa aatttatgag aaacctttac 3150

 atgagcctga cgtcatcgtt tatgcgtaag cgtttagaag ctcctacttt 3200

 gaagagatat ttgcgcgata atatctctaa tattttgcca aatgaagtgc 3250

 ctggtacatc agatgacagt actgaagagc cagtaacgaa aaaacgtact 3300

 tactgtactt actgcccctc taaaataagg cgaaaggcaa atgcatcgtg 3350

 caaaaaatgc aaaaaagtta tttgtcgaga gcataatatt gatatgtgcc 3400

 aaagttgttt ctgagaattc gctagctcga catgataaga tacattgatg 3450

 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 3500

 gaaatttgtg atgctattgc tttatttgtg aaatttgtga tgctattgct 3550

 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg 3600

 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 3650

 gcaagtaaaa cctctacaaa tgtggtagat ccatttaaat gttaattaac 3700

 tagccatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3750

 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 3800

 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 3850

 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 3900

 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 3950

 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 4000

 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 4050

 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 4100

 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 4150

 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 4200

 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 4250

 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 4300

 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 4350

 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 4400

 ggccttttgc tggccttttg ctcacatgtt cttaattaaa tttttcaaaa 4450

 gtagttgaca attaatcatc ggcatagtat atcggcatag tataatacga 4500

 ctcactatag gagggccatc atggccaagt tgaccagtgc tgtcccagtg 4550

 ctcacagcca gggatgtggc tggagctgtt gagttctgga ctgacaggtt 4600

 ggggttctcc agagattttg tggaggatga ctttgcaggt gtggtcagag 4650

 atgatgtcac cctgttcatc tcagcagtcc aggaccaggt ggtgcctgac 4700

 aacaccctgg cttgggtgtg ggtgagagga ctggatgagc tgtatgctga 4750

 gtggagtgag gtggtctcca ccaacttcag ggatgccagt ggccctgcca 4800

 tgacagagat tggagagcag ccctggggga gagagtttgc cctgagagac 4850

 ccagcaggca actgtgtgca ctttgtggca gaggagcagg actgaggata 4900

 agaattgagt ttcagaaaag ggggcctgag tggccccttt tttcaactta 4950

 attaannnnn  nnnnn 4965

<210> 19
<211> 68
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 19
 acgtgaaatc cctatcagtg atagagactt ataagttccc tatcagtgat 50

 agagatctaa  agggaaaa 68

<210> 20
<211> 72
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 20
 agctttttcc ctttagatct ctatcactga tagggaactt ataagtctct 50

 atcactgata gggatttcac  gt 72

<210> 21
<211> 218
<212> PRT
<213> Artificial sequence

<220>
<223> codon-optimized tetR

<400> 21
 Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu
   1               5                  10                  15

 Leu Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu
                  20                  25                  30

 Ala Gln Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val
                  35                  40                  45

 Lys Asn Lys Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu
                  50                  55                  60

 Asp Arg His His Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp
                  65                  70                  75

 Gln Asp Phe Leu Arg Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu
                  80                  85                  90

 Leu Ser His Arg Asp Gly Ala Lys Val His Leu Gly Thr Arg Pro
                  95                 100                 105

 Thr Glu Lys Gln Tyr Glu Thr Leu Glu Asn Gln Leu Ala Phe Leu
                 110                 115                 120

 Cys Gln Gln Gly Phe Ser Leu Glu Asn Ala Leu Tyr Ala Leu Ser
                 125                 130                 135

 Ala Val Gly His Phe Thr Leu Gly Cys Val Leu Glu Asp Gln Glu
                 140                 145                 150

 His Gln Val Ala Lys Glu Glu Arg Glu Thr Pro Thr Thr Asp Ser
                 155                 160                 165

 Met Pro Pro Leu Leu Arg Gln Ala Ile Glu Leu Phe Asp His Gln
                 170                 175                 180

 Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu Ile Ile Cys
                 185                 190                 195

 Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Ser Ala Tyr Ser
                 200                 205                 210

 Gly Ser Arg Glu Phe Arg Ser Tyr
                 215            

<210> 22
<211> 212
<212> PRT
<213> Artificial sequence

<220>
<223> Puromycin selectable marker

<400> 22
 Met Ile Ser Leu Pro Gln Pro Thr Arg Arg Arg Pro Ser Met Thr
   1               5                  10                  15

 Glu Tyr Lys Pro Thr Val Arg Leu Ala Thr Arg Asp Asp Val Pro
                  20                  25                  30

 Arg Ala Val Arg Thr Leu Ala Ala Ala Phe Ala Asp Tyr Pro Ala
                  35                  40                  45

 Thr Arg His Thr Val Asp Pro Asp Arg His Ile Glu Arg Val Thr
                  50                  55                  60

 Glu Leu Gln Glu Leu Phe Leu Thr Arg Val Gly Leu Asp Ile Gly
                  65                  70                  75

 Lys Val Trp Val Ala Asp Asp Gly Ala Ala Val Ala Val Trp Thr
                  80                  85                  90

 Thr Pro Glu Ser Val Glu Ala Gly Ala Val Phe Ala Glu Ile Gly
                  95                 100                 105

 Pro Arg Met Ala Glu Leu Ser Gly Ser Arg Leu Ala Ala Gln Gln
                 110                 115                 120

 Gln Met Glu Gly Leu Leu Ala Pro His Arg Pro Lys Glu Pro Ala
                 125                 130                 135

 Trp Phe Leu Ala Thr Val Gly Val Ser Pro Asp His Gln Gly Lys
                 140                 145                 150

 Gly Leu Gly Ser Ala Val Val Leu Pro Gly Val Glu Ala Ala Glu
                 155                 160                 165

 Arg Ala Gly Val Pro Ala Phe Leu Glu Thr Ser Ala Pro Arg Asn
                 170                 175                 180

 Leu Pro Phe Tyr Glu Arg Leu Gly Phe Thr Val Thr Ala Asp Val
                 185                 190                 195

 Glu Val Pro Glu Gly Pro Arg Thr Trp Cys Met Thr Arg Lys Pro
                 200                 205                 210

 Gly Ala
        

<210> 23
<211> 65
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 23
 gatcccccga caaccctgct atcatcttca agagagatga tagcagggtt 50

 gtcgtttttt  ggaaa 65

<210> 24
<211> 65
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 24
 agcttttcca aaaaacgaca accctgctat catctctctt gaagatgata 50

 gcagggttgt  cgggg 65

<210> 25
<211> 65
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 25
 gatccccggt tgacgccaaa gaataattca agagattatt ctttggcgtc 50

 aacctttttt  ggaaa 65

<210> 26
<211> 65
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 26
 agcttttcca aaaaaggttg acgccaaaga ataatctctt gaattattct 50

 ttggcgtcaa  ccggg 65

<210> 27
<211> 65
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 27
 gatccccccg gaagactcct gataaattca agagatttat caggagtctt 50

 ccggtttttt  ggaaa 65

<210> 28
<211> 65
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 28
 agcttttcca aaaaaccgga agactcctga taaatctctt gaatttatca 50

 ggagtcttcc  ggggg 65

