                         SEQUENCE LISTING

<110>  GEN-PROBE INCORPORATED
       BECKER, Michael M.
       LAM, Wai-Chung M.
       LIVEZEY, Kristin W.
       BRENTANO, Steven T.
       KOLK, Daniel P.
       SCHRODER, Astrid R.W.
 
<120>  METHODS AND KITS FOR AMPLIFYING DNA

<130>  GP147-PCT2

<140>  To Be Assigned
<141>  2007-03-01

<160>  59    

<170>  PatentIn version 3.3

<210>  1
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Insertion sequence for promoter oligonucleotide

<400>  1
ccacaa                                                                 6


<210>  2
<211>  11
<212>  DNA
<213>  Artificial

<220>
<223>  Insertion sequence for promoter oligonucleotide

<400>  2
acgtagcatc c                                                          11


<210>  3
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  T7 RNA polymerase promoter sequence

<400>  3
aatttaatac gactcactat agggaga                                         27


<210>  4
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Target binding portion of promoter oligonucleotide for 5' 
       untranslated region of HCV

<400>  4
ctagccatgg cgttagtatg agtgtcgtgc ag                                   32


<210>  5
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter oligonucleotide for 5' untranslated region of HCV

<400>  5
aatttaatac gactcactat agggagacta gccatggcgt tagtatgagt gtcgtgcag      59


<210>  6
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Priming oligonucleotide for 5' untranslated region of HCV

<400>  6
aggcattgag cgggttgatc caagaaagga c                                    31


<210>  7
<211>  15
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for 5' untranslated region of HCV


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  2'O-methylribonucleotides

<400>  7
guacucaccg guucc                                                      15


<210>  8
<211>  22
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for 5' untranslated region of HCV


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  2'-O-methylribonucleotides

<400>  8
agaccacuau ggcucucccg gg                                              22


<210>  9
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Target binding portionof promoter oligonucleotide for HIV pol 
       gene

<400>  9
acaaatggca gtattcatcc acaatttaaa a                                    31


<210>  10
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter oligonucleotide for HIV pol gene

<400>  10
aatttaatac gactcactat agggagacta gccatggcgt tagtatgagt gtcgtgcag      59


<210>  11
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Priming oligoncueleotide for HIV pol gene

<400>  11
gtttgtatgt ctgttgctat tatgtct                                         27


<210>  12
<211>  17
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for HIV pol gene


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  2'-O-methylribonucleotides

<400>  12
acuguacccc ccaaucc                                                    17


<210>  13
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Target binding portion of promoter oligonucleotide for E6 and E7 
       genes of HPV

<400>  13
gaacagatgg ggcacacaat tcctagt                                         27


<210>  14
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter oligonucleotide for E6 and E7 genes of HPV

<400>  14
aatttaatac gactcactat agggagagaa cagatggggc acacaattcc tagt           54


<210>  15
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Priming oligonucleotide for E6 and E7 genes of HPV

<400>  15
gacagctcag aggaggagg                                                  19


<210>  16
<211>  19
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for E6 and E7 genes of HPV


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  2'-O-methylribonucleotides

<400>  16
ggacaagcag aaccggaca                                                  19


<210>  17
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Target binding portion of promoter oligonucleotide for WNV 
       nonstructural protein S gene

<400>  17
gagtagacgg tgctgcctgc gactcaa                                         27


<210>  18
<211>  58
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter oligonucleotide for WNV nonstructural protein S gene

<400>  18
aatttaatac gactcactca ctatagggag agagtagacg gtgctgcctg cgactcaa       58


<210>  19
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Priming oligonucleotide for WNV nonstructural protein S gene

<400>  19
tccgagacgg ttctgagggc tta                                             23


<210>  20
<211>  18
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for WNV nonstructural protein S gene


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  2'-O-methylribonucleotides

<400>  20
gaucacuucg cggcuuug                                                   18


<210>  21
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Target binding portion of promoter oligonucleotide for 23S rRNA 
       of Chlamydia trachomatis

<400>  21
cggagtaagt taagcacgcg gacgattgga                                      30


<210>  22
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter oligonucleotide for 23S rRNA of Chlamydia trachomatis

<400>  22
aatttaatac gactcactat agggagacgg agtaagttaa gcacgcggac gattgga        57


<210>  23
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Priming oligonucleotide for 23S rRNA of Chlamydia trachomatis

<400>  23
cccgaagatt ccccttgatc gcgacctga                                       29


<210>  24
<211>  24
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for 23S rRNA of Chlamydia trachomatis


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  2'-O-methylribonucleotides

<400>  24
cguucucauc gcucuacgga cucu                                            24


<210>  25
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Target binding portion of promoter oligonucleotide for 16S rRNA 
       of Mycobacterium tuberculosis

<400>  25
actgggtcta ataccggata ggaccacggg atgcat                               36


<210>  26
<211>  64
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis

<400>  26
aattctaata cgactcacta tagggagaac tgggtctaat accggatagg accacgggat     60

gcat                                                                  64


<210>  27
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Target binding portion of promoter oligonucleotide for 16S rRNA 
       of Mycobacterium tuberculosis

<400>  27
actgggtcta ataccggata ggaccacggg a                                    31


<210>  28
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis

<400>  28
aattctaata cgactcacta tagggagaac tgggtctaat accggatagg accacggga      59


<210>  29
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Priming oligonucleotide and detection probe for 16S rRNA of 
       Mycobacterium tuberculosis

<400>  29
gccgtcaccc caccaacaag ctgatag                                         27


<210>  30
<211>  22
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for 16S rRNA of Mycobacterium tuberculosis


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  2'-O-methylribonucleotides

<400>  30
gcucauccca caccgcuaaa gc                                              22


<210>  31
<211>  28
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for 16S rRNA of Mycobacterium tuberculosis


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  2'-O-methylribonucleotides

<400>  31
ccgagauccc acaccgcuaa agccucgg                                        28


<210>  32
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Detection probe for 16S rRNA of Mycobacterium tuberculosis

<400>  32
gctcatccca caccgctaaa gc                                              22


<210>  33
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Target binding portion of promoter oligonucleotide for orfX gene 
       of Staphylococcus aureus

<400>  33
tgacccaagg gcaaagcgac tttg                                            24


<210>  34
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter oligonucleotide for orfX gene of Staphylococcus aureus

<400>  34
aatttaatac gactcactat agggagatga cccaagggca aagcgacttt g              51


<210>  35
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Priming oligonucleotide for orfX gene of Staphylococcus aureus

<400>  35
gtgcgtagtt actgcgttgt aagacgtc                                        28


<210>  36
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Priming oligonucleotide for orfX gene of Staphylococcus aureus

<400>  36
ctgaatgata gtgcgtagtt actgcg                                          26


<210>  37
<211>  17
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for orfX gene of Staphylococcus aureus


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  2'-O-methylribonucleotides

<400>  37
ccgucauugg cggauca                                                    17


<210>  38
<211>  24
<212>  RNA
<213>  Artificial

<220>
<223>  Terminating oligonucleotide for 5' untranslated region of HCV


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  2'-O-methylribonucleotides

<400>  38
ggcuagacgc uuucugcgug aaga                                            24


<210>  39
<211>  22
<212>  RNA
<213>  Artificial

<220>
<223>  Terminating oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  2'-O-methylribonucleotides

<400>  39
cccaguuucc caggcuuauc cc                                              22


<210>  40
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  Detection probe for antisense T7 RNA polymerase promoter sequence

<400>  40
atacgactca ctata                                                      15


<210>  41
<211>  22
<212>  RNA
<213>  Artificial

<220>
<223>  Terminating oligonucleotide for 23S rRNA of Chlamydia trachomatis


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  2'-O-methylribonucleotides

<400>  41
uccgucauuc cuucgcuaua gu                                              22


<210>  42
<211>  24
<212>  RNA
<213>  Artificial

<220>
<223>  Terminating oligonucleotide for the 5' untranslated region of HCV


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  2'-O-methylribonucleotides

<400>  42
agacgcuuuc ugcgugaaga cagu                                            24


<210>  43
<211>  24
<212>  RNA
<213>  Artificial

<220>
<223>  Terminating oligonucleotide for the 5' untranslated region of HCV


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  2'-O-methylribonucleotides

<400>  43
cuagacgcuu ucugcgugaa gaca                                            24


<210>  44
<211>  20
<212>  RNA
<213>  Artificial

<220>
<223>  Terminating oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  2'-O-methylribonucleotides

<400>  44
caguuuccca ggcuuauccc                                                 20


<210>  45
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Digestion oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis

<400>  45
gtattagacc cagtttccca ggct                                            24


<210>  46
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Cap for priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis

<400>  46
ctatc                                                                  5


<210>  47
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Looped priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Abasic nucleotides

<400>  47
ctatttnngc cgtcacccca ccaacaagct gatag                                35


<210>  48
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Looped priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (6)..(10)
<223>  Abasic nucleotides

<400>  48
ctatcnnnnn gccgtcaccc caccaacaag ctgatag                              37


<210>  49
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Looped priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (5)..(9)
<223>  Abasic nucleotides

<400>  49
ctatnnnnng ccgtcacccc accaacaagc tgatag                               36


<210>  50
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Looped priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (7)..(11)
<223>  Abasic nucleotides

<400>  50
ctatcannnn ngccgtcacc ccaccaacaa gctgatag                             38


<210>  51
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Looped priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (6)..(9)
<223>  Abasic nucleotides

<400>  51
ctatcnnnng ccgtcacccc accaacaagc tgatag                               36


<210>  52
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Looped priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (7)..(10)
<223>  Abasic nucleotides

<400>  52
ctatcannnn gccgtcaccc caccaacaag ctgatag                              37


<210>  53
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  Looped priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis


<220>
<221>  misc_feature
<222>  (16)..(20)
<223>  Abasic nucleotides

<400>  53
ctatcagctt gttggnnnnn gccgtcaccc caccaacaag ctgatag                   47


<210>  54
<211>  14
<212>  DNA
<213>  Artificial

<220>
<223>  Cap for priming oligonucleotide for 16S rRNA of Mycobacterium 
       tuberculosis

<400>  54
ctatcagctt gttg                                                       14


<210>  55
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Extender oligonucleotide for 5' untranslated region of HCV

<400>  55
cctccaggac cccccctccc gggagagcca ta                                   32


<210>  56
<211>  26
<212>  RNA
<213>  Artificial

<220>
<223>  Terminating oligonucleotide for 5' untranslated region of HCV

<400>  56
auggcuagac gcuuucugcg ugaaga                                          26


<210>  57
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Displacer oligonucleotide for orfX gene of Staphylococcus aureus


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Locked nucleic acids

<400>  57
cuugctcaat taacacaacc cgcatc                                          26


<210>  58
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  Terminating oligonucleotide for orfX gene of Staphylococcus 
       aureus


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Locked nucleic acids

<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  Locked nucleic acids

<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  Locked nucleic acids

<400>  58
ggtcatgcgt tgguu                                                      15


<210>  59
<211>  22
<212>  RNA
<213>  Artificial

<220>
<223>  Detection probe for orfX gene of Staphylococcus aureus


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  2'-O-methylribonucleotides

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  Nine carbon linker positioned between the nucleotides 17 and 18

<400>  59
ccgucauugg cggaucagac gg                                              22


