
                                SEQUENCE LISTING


<110> Cheek, Robert
      Fischer, Timothy J.
      Groelke, John W.


<120> HE4 MONOCLONAL ANTIBODIES AND METHODS
  FOR THEIR USE

<130> 46143/341087

<150> 60/906,126          
<151> 2007-03-09  

<160> 16

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 124
<212> PRT
<213> Homo sapiens

<220> 
<221> PEPTIDE        
<222> (1)...(124)
<223> HE4 isoform 1

<400> 1
Met Pro Ala Cys Arg Leu Gly Pro Leu Ala Ala Ala Leu Leu Leu Ser
 1               5                  10                  15      
Leu Leu Leu Phe Gly Phe Thr Leu Val Ser Gly Thr Gly Ala Glu Lys
            20                  25                  30          
Thr Gly Val Cys Pro Glu Leu Gln Ala Asp Gln Asn Cys Thr Gln Glu
        35                  40                  45              
Cys Val Ser Asp Ser Glu Cys Ala Asp Asn Leu Lys Cys Cys Ser Ala
    50                  55                  60                  
Gly Cys Ala Thr Phe Cys Ser Leu Pro Asn Asp Lys Glu Gly Ser Cys
65                  70                  75                  80  
Pro Gln Val Asn Ile Asn Phe Pro Gln Leu Gly Leu Cys Arg Asp Gln
                85                  90                  95      
Cys Gln Val Asp Ser Gln Cys Pro Gly Gln Met Lys Cys Cys Arg Asn
            100                 105                 110         
Gly Cys Gly Lys Val Ser Cys Val Thr Pro Asn Phe
        115                 120                 


<210> 2
<211> 570
<212> DNA
<213> Homo sapiens

<220> 
<221> CDS            
<222> (29)...(403)

<400> 2
cacctgcacc ccgcccgggc atagcacc atg cct gct tgt cgc cta ggc ccg    52
                               Met Pro Ala Cys Arg Leu Gly Pro
                                1               5
 
cta gcc gcc gcc ctc ctc ctc agc ctg ctg ctg ttc ggc ttc acc cta   100
Leu Ala Ala Ala Leu Leu Leu Ser Leu Leu Leu Phe Gly Phe Thr Leu
     10                  15                  20
 
gtc tca ggc aca gga gca gag aag act ggc gtg tgc ccc gag ctc cag   148
Val Ser Gly Thr Gly Ala Glu Lys Thr Gly Val Cys Pro Glu Leu Gln
 25                  30                  35                  40
 
gct gac cag aac tgc acg caa gag tgc gtc tcg gac agc gaa tgc gcc   196
Ala Asp Gln Asn Cys Thr Gln Glu Cys Val Ser Asp Ser Glu Cys Ala
                 45                  50                  55
 
gac aac ctc aag tgc tgc agc gcg ggc tgt gcc acc ttc tgc tct ctg   244
Asp Asn Leu Lys Cys Cys Ser Ala Gly Cys Ala Thr Phe Cys Ser Leu
             60                  65                  70
 
ccc aat gat aag gag ggt tcc tgc ccc cag gtg aac att aac ttt ccc   292
Pro Asn Asp Lys Glu Gly Ser Cys Pro Gln Val Asn Ile Asn Phe Pro
         75                  80                  85
 
cag ctc ggc ctc tgt cgg gac cag tgc cag gtg gac agc cag tgt cct   340
Gln Leu Gly Leu Cys Arg Asp Gln Cys Gln Val Asp Ser Gln Cys Pro
     90                  95                 100
 
ggc cag atg aaa tgc tgc cgc aat ggc tgt ggg aag gtg tcc tgt gtc   388
Gly Gln Met Lys Cys Cys Arg Asn Gly Cys Gly Lys Val Ser Cys Val
105                 110                 115                 120
 
act ccc aat ttc tga gctccagcca ccaccaggct gagcagtgag gagagaaagt   443
Thr Pro Asn Phe  *

 
ttctgcctgg ccctgcatct ggttccagcc cacctgccct cccctttttc gggactctgt 503
attccctctt gggctgacca cagcttctcc ctttcccaac caataaagta accactttca 563
gcaaaaa                                                           570

<210> 3
<211> 102
<212> PRT
<213> Homo sapiens

<220> 
<221> PEPTIDE        
<222> (1)...(102)
<223> HE4 isoform 2

<400> 3
Met Pro Ala Cys Arg Leu Gly Pro Leu Ala Ala Ala Leu Leu Leu Ser
 1               5                  10                  15      
Leu Leu Leu Phe Gly Phe Thr Leu Val Ser Gly Thr Gly Ala Glu Lys
            20                  25                  30          
Thr Gly Val Cys Pro Glu Leu Gln Ala Asp Gln Asn Cys Thr Gln Glu
        35                  40                  45              
Cys Val Ser Asp Ser Glu Cys Ala Asp Asn Leu Lys Cys Cys Ser Ala
    50                  55                  60                  
Gly Cys Ala Thr Phe Cys Ser Leu Pro Asn Ala Leu Phe His Trp His
65                  70                  75                  80  
Leu Lys Thr Arg Arg Leu Trp Glu Ile Ser Gly Pro Arg Pro Arg Arg
                85                  90                  95      
Pro Thr Trp Asp Ser Ser
            100         


<210> 4
<211> 358
<212> DNA
<213> Homo sapiens

<220> 
<221> CDS            
<222> (29)...(337)

<400> 4
cacctgcacc ccgcccgggc atagcacc atg cct gct tgt cgc cta ggc ccg    52
                               Met Pro Ala Cys Arg Leu Gly Pro
                                1               5
 
cta gcc gcc gcc ctc ctc ctc agc ctg ctg ctg ttc ggc ttc acc cta   100
Leu Ala Ala Ala Leu Leu Leu Ser Leu Leu Leu Phe Gly Phe Thr Leu
     10                  15                  20
 
gtc tca ggc aca gga gca gag aag act ggc gtg tgc ccc gag ctc cag   148
Val Ser Gly Thr Gly Ala Glu Lys Thr Gly Val Cys Pro Glu Leu Gln
 25                  30                  35                  40
 
gct gac cag aac tgc acg caa gag tgc gtc tcg gac agc gaa tgc gcc   196
Ala Asp Gln Asn Cys Thr Gln Glu Cys Val Ser Asp Ser Glu Cys Ala
                 45                  50                  55
 
gac aac ctc aag tgc tgc agc gcg ggc tgt gcc acc ttc tgc tct ctg   244
Asp Asn Leu Lys Cys Cys Ser Ala Gly Cys Ala Thr Phe Cys Ser Leu
             60                  65                  70
 
ccc aat gca ctg ttc cac tgg cac cta aag aca cgg agg ctc tgg gag   292
Pro Asn Ala Leu Phe His Trp His Leu Lys Thr Arg Arg Leu Trp Glu
         75                  80                  85
 
att tct ggc cct agg cca cga agg ccc act tgg gac tca agc tga       337
Ile Ser Gly Pro Arg Pro Arg Arg Pro Thr Trp Asp Ser Ser  *
     90                  95                 100
 
ggtcctgtga ttccatttgg g                                           358

<210> 5
<211> 79
<212> PRT
<213> Homo sapiens

<220> 
<221> PEPTIDE        
<222> (1)...(79)
<223> HE4 isoform 3

<400> 5
Met Pro Ala Cys Arg Leu Gly Pro Leu Ala Ala Ala Leu Leu Leu Ser
 1               5                  10                  15      
Leu Leu Leu Phe Gly Phe Thr Leu Val Ser Gly Thr Gly Ala Glu Lys
            20                  25                  30          
Thr Gly Val Cys Pro Glu Leu Gln Ala Asp Gln Asn Cys Thr Gln Glu
        35                  40                  45              
Cys Val Ser Asp Ser Glu Cys Ala Asp Asn Leu Lys Cys Cys Ser Ala
    50                  55                  60                  
Gly Cys Ala Thr Phe Cys Ser Leu Pro Asn Gly Gln Leu Ala Glu
65                  70                  75                  


<210> 6
<211> 694
<212> DNA
<213> Homo sapiens

<220> 
<221> CDS            
<222> (29)...(268)

<400> 6
cacctgcacc ccgcccgggc atagcacc atg cct gct tgt cgc cta ggc ccg    52
                               Met Pro Ala Cys Arg Leu Gly Pro
                                1               5
 
cta gcc gcc gcc ctc ctc ctc agc ctg ctg ctg ttc ggc ttc acc cta   100
Leu Ala Ala Ala Leu Leu Leu Ser Leu Leu Leu Phe Gly Phe Thr Leu
     10                  15                  20
 
gtc tca ggc aca gga gca gag aag act ggc gtg tgc ccc gag ctc cag   148
Val Ser Gly Thr Gly Ala Glu Lys Thr Gly Val Cys Pro Glu Leu Gln
 25                  30                  35                  40
 
gct gac cag aac tgc acg caa gag tgc gtc tcg gac agc gaa tgc gcc   196
Ala Asp Gln Asn Cys Thr Gln Glu Cys Val Ser Asp Ser Glu Cys Ala
                 45                  50                  55
 
gac aac ctc aag tgc tgc agc gcg ggc tgt gcc acc ttc tgc tct ctg   244
Asp Asn Leu Lys Cys Cys Ser Ala Gly Cys Ala Thr Phe Cys Ser Leu
             60                  65                  70
 
ccc aat ggc caa ctg gct gag tga ttcgaagaaa gtgaggaatc ctccctggac  298
Pro Asn Gly Gln Leu Ala Glu  *
         75
 
actgtatcgc ccttcgtcgt ctttcagtca atctcttcca ctctaaggat tgagtgagcg 358
cgagctgggg actctctcaa agataaggag ggttcctgcc cccaggtgaa cattaacttt 418
ccccagctcg gcctctgtcg ggaccagtgc caggtggaca gccagtgtcc tggccagatg 478
aaatgctgcc gcaatggctg tgggaaggtg tcctgtgtca ctcccaattt ctgaggtcca 538
gccaccacca ggctgagcag tgaggagaga aagtttctgc ctggccctgc atctggttcc 598
agcccacctg ccctcccctt tttcgggact ctgtattccc tcttgggctg accacagctt 658
ctccctttcc caaccaataa agtaaccact ttcagc                           694

<210> 7
<211> 76
<212> PRT
<213> Homo sapiens

<220> 
<221> PEPTIDE        
<222> (1)...(76)
<223> HE4 isoform 4

<400> 7
Met Pro Ala Cys Arg Leu Gly Pro Leu Ala Ala Ala Leu Leu Leu Ser
 1               5                  10                  15      
Leu Leu Leu Phe Gly Phe Thr Leu Val Ser Asp Lys Glu Gly Ser Cys
            20                  25                  30          
Pro Gln Val Asn Ile Asn Phe Pro Gln Leu Gly Leu Cys Arg Asp Gln
        35                  40                  45              
Cys Gln Val Asp Ser Gln Cys Pro Gly Gln Met Lys Cys Cys Arg Asn
    50                  55                  60                  
Gly Cys Gly Lys Val Ser Cys Val Thr Pro Asn Phe
65                  70                  75      


<210> 8
<211> 421
<212> DNA
<213> Homo sapiens

<220> 
<221> CDS            
<222> (29)...(259)

<400> 8
cacctgcacc ccgcccgggc atagcacc atg cct gct tgt cgc cta ggc ccg    52
                               Met Pro Ala Cys Arg Leu Gly Pro
                                1               5
 
cta gcc gcc gcc ctc ctc ctc agc ctg ctg ctg ttc ggc ttc acc cta   100
Leu Ala Ala Ala Leu Leu Leu Ser Leu Leu Leu Phe Gly Phe Thr Leu
     10                  15                  20
 
gtc tca gat aag gag ggt tcc tgc ccc cag gtg aac att aac ttt ccc   148
Val Ser Asp Lys Glu Gly Ser Cys Pro Gln Val Asn Ile Asn Phe Pro
 25                  30                  35                  40
 
cag ctc ggc ctc tgt cgg gac cag tgc cag gtg gac agc cag tgt cct   196
Gln Leu Gly Leu Cys Arg Asp Gln Cys Gln Val Asp Ser Gln Cys Pro
                 45                  50                  55
 
ggc cag atg aaa tgc tgc cgc aat ggc tgt ggg aag gtg tcc tgt gtc   244
Gly Gln Met Lys Cys Cys Arg Asn Gly Cys Gly Lys Val Ser Cys Val
             60                  65                  70
 
act ccc aat ttc tga ggtccagcca ccaccaggct gagcagtgag gagagaaagt   299
Thr Pro Asn Phe  *
         75
 
ttctgcctgg ccctgcatct ggttccagcc cacctgccct cccctttttc gggactctgt 359
attccctctt gggctgacca cagcttctcc ctttcccaac caataaagta accactttca 419
gc                                                                421

<210> 9
<211> 73
<212> PRT
<213> Homo sapiens

<220> 
<221> PEPTIDE        
<222> (1)...(73)
<223> HE4 isoform 5

<400> 9
Met Leu Gln Val Gln Val Asn Leu Pro Val Ser Pro Leu Pro Thr Tyr
 1               5                  10                  15      
Pro Tyr Ser Phe Phe Tyr Pro Asp Lys Glu Gly Ser Cys Pro Gln Val
            20                  25                  30          
Asn Ile Asn Phe Pro Gln Leu Gly Leu Cys Arg Asp Gln Cys Gln Val
        35                  40                  45              
Asp Ser Gln Cys Pro Gly Gln Met Lys Cys Cys Arg Asn Gly Cys Gly
    50                  55                  60                  
Lys Val Ser Cys Val Thr Pro Asn Phe
65                  70              


<210> 10
<211> 411
<212> DNA
<213> Homo sapiens

<220> 
<221> CDS            
<222> (28)...(249)

<400> 10
agcccagtga ggggcagtgg gggggcc atg ctg cag gta caa gtt aat ctc cct 54
                              Met Leu Gln Val Gln Val Asn Leu Pro
                               1               5
 
gta tcg cct ctg ccc act tac cct tac tcc ttt ttc tac cca gat aag   102
Val Ser Pro Leu Pro Thr Tyr Pro Tyr Ser Phe Phe Tyr Pro Asp Lys
 10                  15                  20                  25
 
gag ggt tcc tgc ccc cag gtg aac att aac ttt ccc cag ctc ggc ctc   150
Glu Gly Ser Cys Pro Gln Val Asn Ile Asn Phe Pro Gln Leu Gly Leu
                 30                  35                  40
 
tgt cgg gac cag tgc cag gtg gac agc cag tgt cct ggc cag atg aaa   198
Cys Arg Asp Gln Cys Gln Val Asp Ser Gln Cys Pro Gly Gln Met Lys
             45                  50                  55
 
tgc tgc cgc aat ggc tgt ggg aag gtg tcc tgt gtc act ccc aat ttc   246
Cys Cys Arg Asn Gly Cys Gly Lys Val Ser Cys Val Thr Pro Asn Phe
         60                  65                  70
 
tga ggtccagcca ccaccaggct gagcagtgag gagagaaagt ttctgcctgg        299
 *

 
ccctgcatct ggttccagcc cacctgccct cccctttttc gggactctgt attccctctt 359
gggctgacca cagcttctcc ctttcccaac caataaagta accactttca gc         411

<210> 11
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Epitope sequence in HE4 for monoclonal antibody
      363A90.1

<400> 11
Val Asn Ile Asn Phe Pro Gln Leu Gly Leu Cys Arg
 1               5                  10          


<210> 12
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> Epitope sequence in HE4 for monoclonal antibody
      363A71.1

<400> 12
Cys Arg Asp Gln Cys Gln Val Asp Ser Gln Cys Pro Gly Gln Met Lys
 1               5                  10                  15      
Cys Cys Arg Asn Gly Cys Gly Lys
            20                  


<210> 13
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> Epitope sequence in HE4 for monoclonal antibody
      363A71.1 (putative refined epitope sequence)

<400> 13
Cys Arg Asp Gln Cys Gln Val Asp Ser Gln Cys Pro Gly Gln Met Lys
 1               5                  10                  15      
Cys Cys Arg Asn
            20  


<210> 14
<211> 27
<212> PRT
<213> Artificial Sequence

<220> 
<223> HE4 fragment comprising five amino acids of region
      4 and all of region 5

<221> PEPTIDE        
<222> (1)...(5)
<223> Portion of region 4 of HE4

<221> PEPTIDE        
<222> (6)...(27)
<223> Region 5 of HE4

<400> 14
Lys Glu Gly Ser Cys Pro Gln Val Asn Ile Asn Phe Pro Gln Leu Gly
 1               5                  10                  15      
Leu Cys Arg Asp Gln Cys Gln Val Asp Ser Gln
            20                  25          


<210> 15
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> HE4 fragment comprising five amino acids of region
      4 and seven amino acids of region 5 [fragment 5Q1]

<221> PEPTIDE        
<222> (1)...(5)
<223> Five amino acids of region 4 of HE4

<221> PEPTIDE        
<222> (6)...(12)
<223> Seven amino acids of region 5 of HE4

<400> 15
Lys Glu Gly Ser Cys Pro Gln Val Asn Ile Asn Phe
 1               5                  10          


<210> 16
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> HE4 fragment comprising twelve amino acids of
      region 5 [fragment 5Q2]

<221> PEPTIDE        
<222> (1)...(12)
<223> Twelve amino acids of region 5 of HE4

<400> 16
Gly Leu Cys Arg Asp Gln Cys Gln Val Asp Ser Gln
 1               5                  10          

