                         SEQUENCE LISTING

<110>  CHEUNG, Nai-Kong V.
 
<120>  USES OF MONOCLONAL ANTIBODY 8H9

<130>  638-F-PCT


<150>  60/896,416
<151>  2007-03-22

<150>  60/915,672
<151>  2007-05-02


<160>  15


<210> 1
<211> 5
<212> PRT
<213> Mus musculus

<400>  1

Asn Tyr Asp Ile Asn 
1               5   


<210>  2
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  2

Trp Ile Phe Pro Gly Asp Gly Ser Thr Gln Tyr 
                 5                   10      


<210>  3
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  3

Gln Thr Thr Ala Thr Trp Phe Ala Tyr 
1               5                   


<210>  4
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  4

Arg Ala Ser Gln Ser Ile Ser Asp Tyr Leu His 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  5

Tyr Ala Ser Gln Ser Ile Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  6

Gln Asn Gly His Ser Phe Pro Leu Thr 
1               5                   


<210>  7
<211>  243
<212>  PRT
<213>  Mus musculus

<400>  7

Gln Val Lys Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly 
                 5                   10                  15

Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                 20                  25                  30

Asn Tyr Asp Ile Asn Trp Val Arg Gln Arg Pro Glu Gln Gly Leu   
                 35                  40                  45

Glu Trp Ile Gly Trp Ile Phe Pro Gly Asp Gly Ser Thr Gln Tyr
                 50                  55                  60

Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Thr Asp Thr Ser 
                 65                  70                  75

Ser Ser Thr Ala Tyr Met Gln Leu Ser Arg Leu Thr Ser Glu Asp
                 80                  85                  90

Ser Ala Val Tyr Phe Cys Ala Arg Gln Thr Thr Ala Thr Trp Phe
                 95                 100                 105

Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly
                110                 115                 120

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile
                125                 130                 135

Glu Leu Thr Gln Ser Pro Thr Thr Leu Ser Val Thr Pro Gly Asp
                140                 145                 150

Arg Val Ser Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asp Tyr
                155                 160                 165

Leu His Trp Tyr Gln Gln Lys Ser His Glu Ser Pro Arg Leu Leu
                170                 175                 180

Ile Lys Tyr Ala Ser Gln Ser Ile Ser Gly Ile Pro Ser Arg Phe
                185                 190                 195

Ser Gly Ser Gly Ser Gly Ser Asp Phe Thr Leu Ser Ile Asn Ser
                200                 205                 210

Val Glu Pro Glu Asp Val Gly Val Tyr Tyr Cys Gln Asn Gly His
                215                 220                 225

Ser Phe Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
                230                 235                 240

Gln Ala Ala    
        

<210>  8
<211>  731
<212>  DNA
<213>  Mus musculus

<400>  8
caggtcaaac tgcagcagtc tggggctgaa ctggtaaagc ctggggcttc agtgaaattg 60

tcctgcaagg cttctggcta caccttcaca aactatgata taaactgggt gaggcagagg 120

cctgaacagg gacttgagtg gattggatgg atttttcctg gagatggtag tactcaatac 180
	
aatgagaagt tcaagggcaa ggccacactg actacagaca catcctccag cacagcctac 240

atgcagctca gcaggctgac atctgaggac tctgctgtct atttctgtgc aagacagact 300

acggctacct ggtttgctta ctggggccaa gggaccacgg tcaccgtctc ctcaggtgga 360
	
ggcggttcag gcggaggtgg ctctggcggt ggcggatcgg acatcgagct cactcagtct 420

ccaaccaccc tgtctgtgac tccaggagat agagtctctc tttcctgcag ggccagccag 480

agtattagcg actacttaca ctggtaccaa caaaaatcac atgagtctcc aaggcttctc 540
	
atcaaatatg cttcccaatc catctctggg atcccctcca ggttcagtgg cagtggatca 600

gggtcagatt tcactctcag tatcaacagt gtggaacctg aagatgttgg agtgtattac 660

tgtcaaaatg gtcacagctt tccgctcacg ttcggtgctg ggaccaagct ggagctgaaa 720
	
caggcggccg c                                                      731


<210>  9
<211>  731
<212>  DNA
<213>  Mus musculus

<400>  9

gtccagtttg acgtcgtcag accccgactt gaccatttcg gaccccgaag tcactttaac  60

aggacgttcc gaagaccgat gtggaagtgt ttgatactat atttgaccca ctccgtctcc  120

ggacttgtcc ctgaactcac ctaacctacc taaaaaggac ctctaccatc atgagttatg  180

ttactcttca agttcccgtt ccggtgtgac tgatgtctgt gtaggaggtc gtgtcggatg  240

tacgtcgagt cgtccgactg tagactcctg agacgacaga taaagacacg ttctgtctga  300

tgccgatgga ccaaacgaat gaccccggtt ccctggtgcc agtggcagag gagtccacct  360

ccgccaagtc cgcctccacc gagaccgcca ccgcctagcc tgtagctcga gtgagtcaga  420

ggttggtggg acagacactg aggtcctcta tctcagagag aaaggacgtc ccggtcggtc  480

tcataatcgc tgatgaatgt gaccatggtt gtttttagtg tactcagagg ttccgaagag  540

tagtttatac gaagggttag gtagagaccc taggggaggt ccaagtcacc gtcacctagt  600

cccagtctaa agtgagagtc atagttgtca caccttggac ttctacaacc tcacataatg  660

acagttttac cagtgtcgaa aggcgagtgc aagccacgac cctggttcga cctcgacttt  720

gtccgccggc g                                                       731


<210>  10
<211>  731
<212>  DNA
<213>  Mus musculus

<400>  10
caggtcaaac tgcagcagtc tggggctgaa ctggtaaagc ctggggcttc agtgaaattg     60

tcctgcaagg cttctggcta caccttcaca aactatgata taaactgggt gaggcagagg    120

cctgaacagg gacttgagtg gattggatgg atttttcctg gagatggtag tactcaatac    180

aatgagaagt tcaagggcaa ggccacactg actacagaca catcctccag cacagcctac    240

atgcagctca gcaggctgac atctgaggac tctgctgtct atttctgtgc aagacagact    300

acggctacct ggtttgctta ctggggccaa gggaccacgg tcaccgtctc ctcagatgga    360

ggcggttcag gcggaggtgg ctctggcggt ggcggatcgg acatcgagct cactcagtct    420

ccaaccaccc tgtctgtgac tccaggagat agagtctctc tttcctgcag ggccagccag    480

agtattagcg actacttaca ctggtaccaa caaaaatcac atgagtctcc aaggcttctc    540

atcaaatatg cttcccaatc catctctggg atcccctcca ggttcagtgg cagtggatca    600

gggtcagatt tcactctcag tatcaacagt gtggaacctg aagatgttgg agtgtattac    660

tgtcaaaatg gtcacagctt tccgctcacg ttcggtgctg ggaccaagct ggagctgaaa    720

caggcggccg c                                                         731


<210>  11
<211>  243
<212>  PRT
<213>  Mus musculus

<400>  11

Gln Val Lys Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly 
                 5                   10                  15

Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                 20                  25                  30

Asn Tyr Asp Ile Asn Trp Val Arg Gln Arg Pro Glu Gln Gly Leu 
                 35                  40                  45

Glu Trp Ile Gly Trp Ile Phe Pro Gly Asp Gly Ser Thr Gln Tyr 
                 50                  55                  60

Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Thr Asp Thr Ser 
                 65                  70                  75

Ser Ser Thr Ala Tyr Met Gln Leu Ser Arg Leu Thr Ser Glu Asp 
                 80                  85                  90

Ser Ala Val Tyr Phe Cys Ala Arg Gln Thr Thr Ala Thr Trp Phe 
                 95                 100                 105

Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Asp Gly 
                110                 115                 120

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
                125                 130                 135

Glu Leu Thr Gln Ser Pro Thr Thr Leu Ser Val Thr Pro Gly Asp 
                140                 145                 150

Arg Val Ser Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asp Tyr 
                155                 160                 165

Leu His Trp Tyr Gln Gln Lys Ser His Glu Ser Pro Arg Leu Leu 
                170                 175                 180

Ile Lys Tyr Ala Ser Gln Ser Ile Ser Gly Ile Pro Ser Arg Phe 
                185                 190                 195

Ser Gly Ser Gly Ser Gly Ser Asp Phe Thr Leu Ser Ile Asn Ser 
                200                 205                 210

Val Glu Pro Glu Asp Val Gly Val Tyr Tyr Cys Gln Asn Gly His 
                215                 220                 225

Ser Phe Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
                230                 235                 240

Gln Ala Ala    
       

<210>  12
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated 8H9 scFv with decreased normal tissue adherence

<400>  12

Gln Val Lys Leu Gln Gln Ser Gly Ala Glu Leu Val Glu Pro Gly 
                 5                   10                  15

Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                 20                  25                  30

Asn Tyr Asp Ile Asn Trp Val Arg Gln Arg Pro Glu Gln Gly Leu 
                 35                  40                  45

Glu Trp Ile Gly Trp Ile Phe Pro Gly Asp Gly Ser Thr Gln Tyr 
                 50                  55                  60

Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Thr Asp Thr Ser 
                 65                  70                  75

Ser Ser Thr Ala Tyr Met Gln Leu Ser Arg Leu Thr Ser Glu Asp 
                 80                  85                  90

Ser Ala Val Tyr Phe Cys Ala Arg Gln Thr Thr Ala Thr Trp Phe 
                 95                 100                 105

Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Asp Gly 
                110                 115                 120

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
                125                 130                 135

Glu Leu Thr Gln Ser Pro Thr Thr Leu Ser Val Thr Pro Gly Asp 
                140                 145                 150

Gln Val Ser Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asp Tyr 
                155                 160                 165

Leu His Trp Tyr Gln Gln Lys Ser His Glu Ser Pro Gln Leu Leu 
                170                 175                 180

Ile Lys Tyr Ala Ser Gln Ser Ile Ser Gly Ile Pro Ser Arg Phe 
                185                 190                 195

Ser Gly Ser Gly Ser Gly Ser Asp Phe Thr Leu Ser Ile Asn Ser 
                200                 205                 210

Val Glu Pro Glu Asp Val Gly Val Tyr Tyr Cys Gln Asn Gly His 
                215                 220                 225

Ser Phe Pro Leu Thr Phe Gly Ala Gly Thr Glu Leu Glu Leu Glu 
                230                 235                 240

Gln Ala Ala   
       

<210>  13
<211>  18
<212>  PRT
<213>  Mus musculus

<400>  13

Asn Pro Val Leu Gln Gln Asp Ala His Ser Ser Val Thr Ile Thr 
                 5                   10                  15
Pro Gln Arg
         

<210>  14
<211>  26
<212>  PRT
<213>  Mus musculus

<400>  14

Ser Pro Thr Gly Ala Val Glu Val Gln Val Pro Glu Asp Pro Val 
                 5                   10                  15
Val Ala Leu Val Gly Thr Asp Ala Thr Leu Arg
                 20                  25


<210>  15
<211>  534
<212>  PRT
<213>  Mus musculus

<400>  15

Met Leu Arg Arg Arg Gly Ser Pro Gly Met Gly Val His Val Gly 
                 5                   10                  15
Ala Ala Leu Gly Ala Leu Trp Phe Cys Leu Thr Gly Ala Leu Glu 
                 20                  25                  30
Val Gln Val Pro Glu Asp Pro Val Val Ala Leu Val Gly Thr Asp 
                 35                  40                  45
Ala Thr Leu Cys Cys Ser Phe Ser Pro Glu Pro Gly Phe Ser Leu 
                 50                  55                  60
Ala Gln Leu Asn Leu Ile Trp Gln Leu Thr Asp Thr Lys Gln Leu 
                 65                  70                  75
Val His Ser Phe Ala Glu Gly Gln Asp Gln Gly Ser Ala Tyr Ala 
                 80                  85                  90
Asn Arg Thr Ala Leu Phe Pro Asp Leu Leu Ala Gln Gly Asn Ala 
                 95                 100                 105
Ser Leu Arg Leu Gln Arg Val Arg Val Ala Asp Glu Gly Ser Phe 
                110                 115                 120
Thr Cys Phe Val Ser Ile Arg Asp Phe Gly Ser Ala Ala Val Ser 
                125                 130                 135
Leu Gln Val Ala Ala Pro Tyr Ser Lys Pro Ser Met Thr Leu Glu 
                140                 145                 150
Pro Asn Lys Asp Leu Arg Pro Gly Asp Thr Val Thr Ile Thr Cys 
                155                 160                 165
Ser Ser Tyr Gln Gly Tyr Pro Glu Ala Glu Val Phe Trp Gln Asp 
                170                 175                 180  
Gly Gln Gly Val Pro Leu Thr Gly Asn Val Thr Thr Ser Gln Met 
                185                 190                 195
Ala Asn Glu Gln Gly Leu Phe Asp Val His Ser Ile Leu Arg Val 
                200                 205                 210
Val Leu Gly Ala Asn Gly Thr Tyr Ser Cys Leu Val Arg Asn Pro 
                215                 220                 225
Val Leu Gln Gln Asp Ala His Ser Ser Val Thr Ile Thr Pro Gln 
                230                 235                 240
Arg Ser Pro Thr Gly Ala Val Glu Val Gln Val Pro Glu Asp Pro 
                245                 250                 255
Val Val Ala Leu Val Gly Thr Asp Ala Thr Leu Arg Cys Ser Phe 
                260                 265                 270
Ser Pro Glu Pro Gly Phe Ser Leu Ala Gln Leu Asn Leu Ile Trp 
                275                 280                 285
Gln Leu Thr Asp Thr Lys Gln Leu Val His Ser Phe Thr Glu Gly 
                290                 295                 300
Arg Asp Gln Gly Ser Ala Tyr Ala Asn Arg Thr Ala Leu Phe Pro 
                305                 310                 315
Asp Leu Leu Ala Gln Gly Asn Ala Ser Leu Arg Leu Gln Arg Val 
                320                 325                 330
Arg Val Ala Asp Glu Gly Ser Phe Thr Cys Phe Val Ser Ile Arg 
                335                 340                 345
Asp Phe Gly Ser Ala Ala Val Ser Leu Gln Val Ala Ala Pro Tyr 
                350                 355                 360
Ser Lys Pro Ser Met Thr Leu Glu Pro Asn Lys Asp Leu Arg Pro 
                365                 370                 375 
Gly Asp Thr Val Thr Ile Thr Cys Ser Ser Tyr Arg Gly Tyr Pro 
                380                 385                 390
Glu Ala Glu Val Phe Trp Gln Asp Gly Gln Gly Val Pro Leu Thr 
                395                 400                 405
Gly Asn Val Thr Thr Ser Gln Met Ala Asn Glu Gln Gly Leu Phe 
                410                 415                 420
Asp Val His Ser Val Leu Arg Val Val Leu Gly Ala Asn Gly Thr 
                425                 430                 435
Tyr Ser Cys Leu Val Arg Asn Pro Val Leu Gln Gln Asp Ala His 
                440                 445                 450
Gly Ser Val Thr Ile Thr Gly Gln Pro Met Thr Phe Pro Pro Glu 
                455                 460                 465
Ala Leu Trp Val Thr Val Gly Leu Ser Val Cys Leu Ile Ala Leu 
                470                 475                 480
Leu Val Ala Leu Ala Phe Val Cys Trp Arg Lys Ile Lys Gln Ser 
                485                 490                 495
Cys Glu Glu Glu Asn Ala Gly Ala Glu Asp Gln Asp Gly Glu Gly 
                500                 505                 510
Glu Gly Ser Lys Thr Ala Leu Gln Pro Leu Lys His Ser Asp Ser 
                515                 520                 525
Lys Glu Asp Asp Gly Gln Glu Ile Ala
                530             




