                         SEQUENCE LISTING

<110>  Cujec, Thomas P.
       Mariani, Roberto
       Hays Putnam, Anna-Maria A.
       Keefe, William  M.
       Knudsen, Nick
       Ho, Lillian
       Pinkstaff, Jason
       Kraynov, Vadim
 
<120>  Modified FGF-21 Polypeptides and Their Uses

<130>  AMBX-0134.00PCT

<150>  60/921,297
<151>  2007-03-30

<150>  60/988,060
<151>  2007-11-14

<160>  44    

<170>  PatentIn version 3.4

<210>  1
<211>  181
<212>  PRT
<213>  Homo sapiens

<400>  1

His Pro Ile Pro Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val 
1               5                   10                  15      


Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala His 
            20                  25                  30          


Leu Glu Ile Arg Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln Ser 
        35                  40                  45              


Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile Gln 
    50                  55                  60                  


Ile Leu Gly Val Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly 
65                  70                  75                  80  


Ala Leu Tyr Gly Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe Arg 
                85                  90                  95      


Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His 
            100                 105                 110         


Gly Leu Pro Leu His Leu Pro Gly Asn Lys Ser Pro His Arg Asp Pro 
        115                 120                 125             


Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro 
    130                 135                 140                 


Ala Pro Pro Glu Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp Val 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg Ser 
                165                 170                 175     


Pro Ser Tyr Ala Ser 
            180     


<210>  2
<211>  191
<212>  PRT
<213>  Homo sapiens

<400>  2

Met His His His His His His Ser Gly Gly His Pro Ile Pro Asp Ser 
1               5                   10                  15      


Ser Pro Leu Leu Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr Leu Tyr 
            20                  25                  30          


Thr Asp Asp Ala Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu Asp 
        35                  40                  45              


Gly Thr Val Gly Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu Gln 
    50                  55                  60                  


Leu Lys Ala Leu Lys Pro Gly Val Ile Gln Ile Leu Gly Val Lys Thr 
65                  70                  75                  80  


Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser Leu 
                85                  90                  95      


His Phe Asp Pro Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu Asp 
            100                 105                 110         


Gly Tyr Asn Val Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His Leu 
        115                 120                 125             


Pro Gly Asn Lys Ser Pro His Arg Asp Pro Ala Pro Arg Gly Pro Ala 
    130                 135                 140                 


Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro Ala Pro Pro Glu Pro Pro 
145                 150                 155                 160 


Gly Ile Leu Ala Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro Leu 
                165                 170                 175     


Ser Met Val Gly Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
            180                 185                 190     


<210>  3
<211>  209
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Asp Ser Asp Glu Thr Gly Phe Glu His Ser Gly Leu Trp Val Ser 
1               5                   10                  15      


Val Leu Ala Gly Leu Leu Leu Gly Ala Cys Gln Ala His Pro Ile Pro 
            20                  25                  30          


Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr 
        35                  40                  45              


Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala His Leu Glu Ile Arg 
    50                  55                  60                  


Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu 
65                  70                  75                  80  


Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile Gln Ile Leu Gly Val 
                85                  90                  95      


Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly 
            100                 105                 110         


Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu 
        115                 120                 125             


Glu Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His Gly Leu Pro Leu 
    130                 135                 140                 


His Leu Pro Gly Asn Lys Ser Pro His Arg Asp Pro Ala Pro Arg Gly 
145                 150                 155                 160 


Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro Ala Pro Pro Glu 
                165                 170                 175     


Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp Val Gly Ser Ser Asp 
            180                 185                 190         


Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala 
        195                 200                 205             


Ser 
    


<210>  4
<211>  208
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Asp Ser Asp Glu Thr Gly Phe Glu His Ser Gly Leu Trp Val Ser 
1               5                   10                  15      


Val Leu Ala Gly Leu Leu Gly Ala Cys Gln Ala His Pro Ile Pro Asp 
            20                  25                  30          


Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr Leu 
        35                  40                  45              


Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu 
    50                  55                  60                  


Asp Gly Thr Val Gly Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu 
65                  70                  75                  80  


Gln Leu Lys Ala Leu Lys Pro Gly Val Ile Gln Ile Leu Gly Val Lys 
                85                  90                  95      


Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser 
            100                 105                 110         


Leu His Phe Asp Pro Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu 
        115                 120                 125             


Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His 
    130                 135                 140                 


Leu Pro Gly Asn Lys Ser Pro His Arg Asp Pro Ala Pro Arg Gly Pro 
145                 150                 155                 160 


Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro Ala Pro Pro Glu Pro 
                165                 170                 175     


Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro 
            180                 185                 190         


Leu Ser Met Val Gly Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
        195                 200                 205             


<210>  5
<211>  181
<212>  PRT
<213>  Homo sapiens

<400>  5

His Pro Ile Pro Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val 
1               5                   10                  15      


Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala His 
            20                  25                  30          


Leu Glu Ile Arg Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln Ser 
        35                  40                  45              


Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile Gln 
    50                  55                  60                  


Ile Leu Gly Val Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly 
65                  70                  75                  80  


Ala Leu Tyr Gly Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe Arg 
                85                  90                  95      


Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His 
            100                 105                 110         


Gly Leu Pro Leu His Leu Pro Gly Asn Lys Ser Pro His Arg Asp Pro 
        115                 120                 125             


Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro 
    130                 135                 140                 


Ala Leu Pro Glu Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp Val 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg Ser 
                165                 170                 175     


Pro Ser Tyr Ala Ser 
            180     


<210>  6
<211>  209
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Asp Ser Asp Glu Thr Gly Phe Glu His Ser Gly Leu Trp Val Ser 
1               5                   10                  15      


Val Leu Ala Gly Leu Leu Leu Gly Ala Cys Gln Ala His Pro Ile Pro 
            20                  25                  30          


Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr 
        35                  40                  45              


Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala His Leu Glu Ile Arg 
    50                  55                  60                  


Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu 
65                  70                  75                  80  


Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile Gln Ile Leu Gly Val 
                85                  90                  95      


Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly 
            100                 105                 110         


Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu 
        115                 120                 125             


Glu Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His Gly Leu Pro Leu 
    130                 135                 140                 


His Leu Pro Gly Asn Lys Ser Pro His Arg Asp Pro Ala Pro Arg Gly 
145                 150                 155                 160 


Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro Ala Leu Pro Glu 
                165                 170                 175     


Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp Val Gly Ser Ser Asp 
            180                 185                 190         


Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala 
        195                 200                 205             


Ser 
    


<210>  7
<211>  208
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Asp Ser Asp Glu Thr Gly Phe Glu His Ser Gly Leu Trp Val Ser 
1               5                   10                  15      


Val Leu Ala Gly Leu Leu Gly Ala Cys Gln Ala His Pro Ile Pro Asp 
            20                  25                  30          


Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val Arg Gln Arg Tyr Leu 
        35                  40                  45              


Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala His Leu Glu Ile Arg Glu 
    50                  55                  60                  


Asp Gly Thr Val Gly Gly Ala Ala Asp Gln Ser Pro Glu Ser Leu Leu 
65                  70                  75                  80  


Gln Leu Lys Ala Leu Lys Pro Gly Val Ile Gln Ile Leu Gly Val Lys 
                85                  90                  95      


Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp Gly Ala Leu Tyr Gly Ser 
            100                 105                 110         


Leu His Phe Asp Pro Glu Ala Cys Ser Phe Arg Glu Leu Leu Leu Glu 
        115                 120                 125             


Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His Gly Leu Pro Leu His 
    130                 135                 140                 


Leu Pro Gly Asn Lys Ser Pro His Arg Asp Pro Ala Pro Arg Gly Pro 
145                 150                 155                 160 


Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro Pro Ala Leu Pro Glu Pro 
                165                 170                 175     


Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp Val Gly Ser Ser Asp Pro 
            180                 185                 190         


Leu Ser Met Val Gly Pro Ser Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
        195                 200                 205             


<210>  8
<211>  546
<212>  DNA
<213>  Homo sapiens

<400>  8
caccccatcc ctgactccag tcctctcctg caattcgggg gccaagtccg gcagcggtac     60

ctctacacag atgatgccca gcagacagaa gcccacctgg agatcaggga ggatgggacg    120

gtggggggcg ctgctgacca gagccccgaa agtctcctgc agctgaaagc cttgaagccg    180

ggagttattc aaatcttggg agtcaagaca tccaggttcc tgtgccagcg gccagatggg    240

gccctgtatg gatcgctcca ctttgaccct gaggcctgca gcttccggga gctgcttctt    300

gaggacggat acaatgttta ccagtccgaa gcccacggcc tcccgctgca cctgccaggg    360

aacaagtccc cacaccggga ccctgcaccc cgaggaccag ctcgcttcct gccactacca    420

ggcctgcccc ccgcaccccc ggagccaccc ggaatcctgg ccccccagcc ccccgatgtg    480

ggctcctcgg accctctgag catggtggga ccttcccagg gccgaagccc cagctacgct    540

tcctga                                                               546


<210>  9
<211>  576
<212>  DNA
<213>  Homo sapiens

<400>  9
atgcatcatc atcatcatca tagcggcggc caccccatcc ctgactccag tcctctcctg     60

caattcgggg gccaagtccg gcagcggtac ctctacacag atgatgccca gcagacagaa    120

gcccacctgg agatcaggga ggatgggacg gtggggggcg ctgctgacca gagccccgaa    180

agtctcctgc agctgaaagc cttgaagccg ggagttattc aaatcttggg agtcaagaca    240

tccaggttcc tgtgccagcg gccagatggg gccctgtatg gatcgctcca ctttgaccct    300

gaggcctgca gcttccggga gctgcttctt gaggacggat acaatgttta ccagtccgaa    360

gcccacggcc tcccgctgca cctgccaggg aacaagtccc cacaccggga ccctgcaccc    420

cgaggaccag ctcgcttcct gccactacca ggcctgcccc ccgcaccccc ggagccaccc    480

ggaatcctgg ccccccagcc ccccgatgtg ggctcctcgg accctctgag catggtggga    540

ccttcccagg gccgaagccc cagctacgct tcctga                              576


<210>  10
<211>  630
<212>  DNA
<213>  Homo sapiens

<400>  10
atggactcgg acgagaccgg gttcgagcac tcaggactgt gggtttctgt gctggctggt     60

cttctgctgg gagcctgcca ggcacacccc atccctgact ccagtcctct cctgcaattc    120

gggggccaag tccggcagcg gtacctctac acagatgatg cccagcagac agaagcccac    180

ctggagatca gggaggatgg gacggtgggg ggcgctgctg accagagccc cgaaagtctc    240

ctgcagctga aagccttgaa gccgggagtt attcaaatct tgggagtcaa gacatccagg    300

ttcctgtgcc agcggccaga tggggccctg tatggatcgc tccactttga ccctgaggcc    360

tgcagcttcc gggagctgct tcttgaggac ggatacaatg tttaccagtc cgaagcccac    420

ggcctcccgc tgcacctgcc agggaacaag tccccacacc gggaccctgc accccgagga    480

ccagctcgct tcctgccact accaggcctg ccccccgcac ccccggagcc acccggaatc    540

ctggcccccc agccccccga tgtgggctcc tcggaccctc tgagcatggt gggaccttcc    600

cagggccgaa gccccagcta cgcttcctga                                     630


<210>  11
<211>  627
<212>  DNA
<213>  Homo sapiens

<400>  11
atggactcgg acgagaccgg gttcgagcac tcaggactgt gggtttctgt gctggctggt     60

cttctgggag cctgccaggc acaccccatc cctgactcca gtcctctcct gcaattcggg    120

ggccaagtcc ggcagcggta cctctacaca gatgatgccc agcagacaga agcccacctg    180

gagatcaggg aggatgggac ggtggggggc gctgctgacc agagccccga aagtctcctg    240

cagctgaaag ccttgaagcc gggagttatt caaatcttgg gagtcaagac atccaggttc    300

ctgtgccagc ggccagatgg ggccctgtat ggatcgctcc actttgaccc tgaggcctgc    360

agcttccggg agctgcttct tgaggacgga tacaatgttt accagtccga agcccacggc    420

ctcccgctgc acctgccagg gaacaagtcc ccacaccggg accctgcacc ccgaggacca    480

gctcgcttcc tgccactacc aggcctgccc cccgcacccc cggagccacc cggaatcctg    540

gccccccagc cccccgatgt gggctcctcg gaccctctga gcatggtggg accttcccag    600

ggccgaagcc ccagctacgc ttcctga                                        627


<210>  12
<211>  545
<212>  DNA
<213>  homo sapiens

<400>  12
caccccatcc ctgactccag tcctctcctg caattcgggg gccaagtccg gcagcggtac     60

ctctacacag atgatgccca gcagacagaa gcccacctgg agatcaggga ggatgggacg    120

gtggggggcg ctgctgacca gagccccgaa agtctcctgc agctgaaagc cttgaagccg    180

ggagttattc aaatcttggg agtcaagaca tccaggttcc tgtgccagcg gccagatggg    240

gccctgtatg gatcgctcca ctttgaccct gaggcctgca gcttccggga gctgcttctt    300

gaggacggat acaatgttta ccagtccgaa gcccacggcc tcccgctgca cctgccaggg    360

aacaagtccc cacaccggga ccctgcaccc cgaggaccag ctcgcttcct gccactacca    420

ggcctgcccc ccgcactccc ggagccaccc ggaatcctgg ccccccagcc ccccgatgtg    480

ggctcctcgg accctctgag catggtggga ccttcccagg gccgaagccc agctacgctt    540

cctga                                                                545


<210>  13
<211>  630
<212>  DNA
<213>  Homo sapiens

<400>  13
atggactcgg acgagaccgg gttcgagcac tcaggactgt gggtttctgt gctggctggt     60

cttctgctgg gagcctgcca ggcacacccc atccctgact ccagtcctct cctgcaattc    120

gggggccaag tccggcagcg gtacctctac acagatgatg cccagcagac agaagcccac    180

ctggagatca gggaggatgg gacggtgggg ggcgctgctg accagagccc cgaaagtctc    240

ctgcagctga aagccttgaa gccgggagtt attcaaatct tgggagtcaa gacatccagg    300

ttcctgtgcc agcggccaga tggggccctg tatggatcgc tccactttga ccctgaggcc    360

tgcagcttcc gggagctgct tcttgaggac ggatacaatg tttaccagtc cgaagcccac    420

ggcctcccgc tgcacctgcc agggaacaag tccccacacc gggaccctgc accccgagga    480

ccagctcgct tcctgccact accaggcctg ccccccgcac tcccggagcc acccggaatc    540

ctggcccccc agccccccga tgtgggctcc tcggaccctc tgagcatggt gggaccttcc    600

cagggccgaa gccccagcta cgcttcctga                                     630


<210>  14
<211>  627
<212>  DNA
<213>  Homo sapiens

<400>  14
atggactcgg acgagaccgg gttcgagcac tcaggactgt gggtttctgt gctggctggt     60

cttctgggag cctgccaggc acaccccatc cctgactcca gtcctctcct gcaattcggg    120

ggccaagtcc ggcagcggta cctctacaca gatgatgccc agcagacaga agcccacctg    180

gagatcaggg aggatgggac ggtggggggc gctgctgacc agagccccga aagtctcctg    240

cagctgaaag ccttgaagcc gggagttatt caaatcttgg gagtcaagac atccaggttc    300

ctgtgccagc ggccagatgg ggccctgtat ggatcgctcc actttgaccc tgaggcctgc    360

agcttccggg agctgcttct tgaggacgga tacaatgttt accagtccga agcccacggc    420

ctcccgctgc acctgccagg gaacaagtcc ccacaccggg accctgcacc ccgaggacca    480

gctcgcttcc tgccactacc aggcctgccc cccgcactcc cggagccacc cggaatcctg    540

gccccccagc cccccgatgt gggctcctcg gaccctctga gcatggtggg accttcccag    600

ggccgaagcc ccagctacgc ttcctga                                        627


<210>  15
<211>  77
<212>  DNA
<213>  Artificial

<220>
<223>  Mutant tRNA derived from Methanococcus jannaschii tRNA

<400>  15
ccggcggtag ttcagcaggg cagaacggcg gactctaaat ccgcatggcg ctggttcaaa     60

tccagcccgc cggacca                                                    77


<210>  16
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant synthetase derived from Methanococcus jannaschii 
       synthetase

<400>  16

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Val 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Tyr Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu His Gly Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Ile His 
145                 150                 155                 160 


Tyr Glu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  17
<211>  77
<212>  DNA
<213>  Methanococcus jannaschii

<400>  17
ccggcggtag ttcagcaggg cagaacggcg gactctaaat ccgcatggcg ctggttcaaa     60

tccggcccgc cggacca                                                    77


<210>  18
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  An optimized amber supressor tRNA

<400>  18
cccagggtag ccaagctcgg ccaacggcga cggactctaa atccgttctc gtaggagttc     60

gagggttcga atcccttccc tgggacca                                        88


<210>  19
<211>  89
<212>  DNA
<213>  Artificial

<220>
<223>  An optimized AGGA frameshift supressor tRNA

<400>  19
gcgagggtag ccaagctcgg ccaacggcga cggacttcct aatccgttct cgtaggagtt     60

cgagggttcg aatccctccc ctcgcacca                                       89


<210>  20
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-L-phenylalanine

<400>  20

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Tyr Tyr 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  21
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-benzoyl-L-phenylalanine

<400>  21

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Ser His 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  22
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  22

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Pro Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ala Ile Tyr 
145                 150                 155                 160 


Leu Ala Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  23
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  23

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Pro Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ile Pro Tyr 
145                 150                 155                 160 


Leu Pro Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  24
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  24

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Lys Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ala Ile Tyr 
145                 150                 155                 160 


Leu Ala Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  25
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  25

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Asn Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Leu His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  26
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  26

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Leu His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  27
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-phenylalanine

<400>  27

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Val His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  28
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  28

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Ser His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  29
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-acetyl-phenylalanine

<400>  29

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Cys His 
145                 150                 155                 160 


Tyr Arg Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  30
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of p-acetyl 
       -phenylalanine

<400>  30

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Thr His 
145                 150                 155                 160 


Tyr Arg Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  31
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-acetyl-phenylalanine

<400>  31

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Gly His 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ile Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  32
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  32

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Arg Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Val Ile His 
145                 150                 155                 160 


Tyr Asp Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  33
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  33

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Tyr Tyr 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  34
<211>  208
<212>  PRT
<213>  Rattus norvegicus

<400>  34

Met Asp Trp Met Lys Ser Arg Val Gly Ala Pro Gly Leu Trp Val Cys 
1               5                   10                  15      


Leu Leu Leu Pro Val Phe Leu Leu Gly Val Cys Glu Ala Tyr Pro Ile 
            20                  25                  30          


Ser Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val Arg Gln Arg 
        35                  40                  45              


Tyr Leu Tyr Thr Asp Asp Asp Gln Asp Thr Glu Ala His Leu Glu Ile 
    50                  55                  60                  


Arg Glu Asp Gly Thr Val Val Gly Thr Ala His Arg Ser Pro Glu Ser 
65                  70                  75                  80  


Leu Leu Glu Leu Lys Ala Leu Lys Pro Gly Val Ile Gln Ile Leu Gly 
                85                  90                  95      


Val Lys Ala Ser Arg Phe Leu Cys Gln Gln Pro Asp Gly Thr Leu Tyr 
            100                 105                 110         


Gly Ser Pro His Phe Asp Pro Glu Ala Cys Ser Phe Arg Glu Leu Leu 
        115                 120                 125             


Leu Lys Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His Gly Leu Pro 
    130                 135                 140                 


Leu Arg Leu Pro Gln Lys Asp Ser Gln Asp Pro Ala Thr Arg Gly Pro 
145                 150                 155                 160 


Val Arg Phe Leu Pro Met Pro Gly Leu Pro His Glu Pro Gln Glu Gln 
                165                 170                 175     


Pro Gly Val Leu Pro Pro Glu Pro Pro Asp Val Gly Ser Ser Asp Pro 
            180                 185                 190         


Leu Ser Met Val Glu Pro Leu Gln Gly Arg Ser Pro Ser Tyr Ala Ser 
        195                 200                 205             


<210>  35
<211>  210
<212>  PRT
<213>  Mus musculus

<400>  35

Met Glu Trp Met Arg Ser Arg Val Gly Thr Leu Gly Leu Trp Val Arg 
1               5                   10                  15      


Leu Leu Leu Ala Val Phe Leu Leu Gly Val Tyr Gln Ala Tyr Pro Ile 
            20                  25                  30          


Pro Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln Val Arg Gln Arg 
        35                  40                  45              


Tyr Leu Tyr Thr Asp Asp Asp Gln Asp Thr Glu Ala His Leu Glu Ile 
    50                  55                  60                  


Arg Glu Asp Gly Thr Val Val Gly Ala Ala His Arg Ser Pro Glu Ser 
65                  70                  75                  80  


Leu Leu Glu Leu Lys Ala Leu Lys Pro Gly Val Ile Gln Ile Leu Gly 
                85                  90                  95      


Val Lys Ala Ser Arg Phe Leu Cys Gln Gln Pro Asp Gly Ala Leu Tyr 
            100                 105                 110         


Gly Ser Pro His Phe Asp Pro Glu Ala Cys Ser Phe Arg Glu Leu Leu 
        115                 120                 125             


Leu Glu Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala His Gly Leu Pro 
    130                 135                 140                 


Leu Arg Leu Pro Gln Lys Asp Ser Pro Asn Gln Asp Ala Thr Ser Trp 
145                 150                 155                 160 


Gly Pro Val Arg Phe Leu Pro Met Pro Gly Leu Leu His Glu Pro Gln 
                165                 170                 175     


Asp Gln Ala Gly Phe Leu Pro Pro Glu Pro Pro Asp Val Gly Ser Ser 
            180                 185                 190         


Asp Pro Leu Ser Met Val Glu Pro Leu Gln Gly Arg Ser Pro Ser Tyr 
        195                 200                 205             


Ala Ser 
    210 


<210>  36
<211>  194
<212>  PRT
<213>  Danio rerio

<400>  36

Met Leu Phe Ala Cys Phe Phe Ile Phe Phe Ala Leu Phe Pro His Leu 
1               5                   10                  15      


Arg Trp Cys Met Tyr Val Pro Ala Gln Asn Val Leu Leu Gln Phe Gly 
            20                  25                  30          


Thr Gln Val Arg Glu Arg Leu Leu Tyr Thr Asp Gly Leu Phe Leu Glu 
        35                  40                  45              


Met Asn Pro Asp Gly Ser Val Lys Gly Ser Pro Glu Lys Asn Leu Asn 
    50                  55                  60                  


Cys Val Leu Glu Leu Arg Ser Val Lys Ala Gly Glu Thr Val Ile Gln 
65                  70                  75                  80  


Ser Ala Ala Thr Ser Leu Tyr Leu Cys Val Asp Asp Gln Asp Lys Leu 
                85                  90                  95      


Lys Gly Gln His His Tyr Ser Ala Leu Asp Cys Thr Phe Gln Glu Leu 
            100                 105                 110         


Leu Leu Asp Gly Tyr Ser Phe Phe Leu Ser Pro His Thr Asn Leu Pro 
        115                 120                 125             


Val Ser Leu Leu Ser Lys Arg Gln Lys His Gly Asn Pro Leu Ser Arg 
    130                 135                 140                 


Phe Leu Pro Val Ser Arg Ala Glu Asp Ser Arg Thr Gln Glu Val Lys 
145                 150                 155                 160 


Gln Tyr Ile Gln Asp Ile Asn Leu Asp Ser Asp Asp Pro Leu Gly Met 
                165                 170                 175     


Gly His Arg Ser His Leu Gln Thr Val Phe Ser Pro Ser Leu His Thr 
            180                 185                 190         


Lys Lys 
        


<210>  37
<211>  1043
<212>  PRT
<213>  Homo sapiens

<400>  37

Met Lys Pro Gly Cys Ala Ala Gly Ser Pro Gly Asn Glu Trp Ile Phe 
1               5                   10                  15      


Phe Ser Thr Asp Glu Ile Thr Thr Arg Tyr Arg Asn Thr Met Ser Asn 
            20                  25                  30          


Gly Gly Leu Gln Arg Ser Val Ile Leu Ser Ala Leu Ile Leu Leu Arg 
        35                  40                  45              


Ala Val Thr Gly Phe Ser Gly Asp Gly Arg Ala Ile Trp Ser Lys Asn 
    50                  55                  60                  


Pro Asn Phe Thr Pro Val Asn Glu Ser Gln Leu Phe Leu Tyr Asp Thr 
65                  70                  75                  80  


Phe Pro Lys Asn Phe Phe Trp Gly Ile Gly Thr Gly Ala Leu Gln Val 
                85                  90                  95      


Glu Gly Ser Trp Lys Lys Asp Gly Lys Gly Pro Ser Ile Trp Asp His 
            100                 105                 110         


Phe Ile His Thr His Leu Lys Asn Val Ser Ser Thr Asn Gly Ser Ser 
        115                 120                 125             


Asp Ser Tyr Ile Phe Leu Glu Lys Asp Leu Ser Ala Leu Asp Phe Ile 
    130                 135                 140                 


Gly Val Ser Phe Tyr Gln Phe Ser Ile Ser Trp Pro Arg Leu Phe Pro 
145                 150                 155                 160 


Asp Gly Ile Val Thr Val Ala Asn Ala Lys Gly Leu Gln Tyr Tyr Ser 
                165                 170                 175     


Thr Leu Leu Asp Ala Leu Val Leu Arg Asn Ile Glu Ile Val Thr Leu 
            180                 185                 190         


Tyr His Trp Asp Leu Pro Leu Ala Leu Gln Glu Lys Tyr Gly Gly Trp 
        195                 200                 205             


Lys Asn Asp Thr Ile Ile Asp Ile Phe Asn Asp Tyr Ala Thr Tyr Cys 
    210                 215                 220                 


Phe Gln Met Phe Gly Asp Arg Val Lys Tyr Trp Ile Thr Ile His Asn 
225                 230                 235                 240 


Pro Tyr Leu Val Ala Trp His Gly Tyr Gly Thr Gly Met His Ala Pro 
                245                 250                 255     


Gly Glu Lys Gly Asn Leu Ala Ala Val Tyr Thr Val Gly His Asn Leu 
            260                 265                 270         


Ile Lys Ala His Ser Lys Val Trp His Asn Tyr Asn Thr His Phe Arg 
        275                 280                 285             


Pro His Gln Lys Gly Trp Leu Ser Ile Thr Leu Gly Ser His Trp Ile 
    290                 295                 300                 


Glu Pro Asn Arg Ser Glu Asn Thr Met Asp Ile Phe Lys Cys Gln Gln 
305                 310                 315                 320 


Ser Met Val Ser Val Leu Gly Trp Phe Ala Asn Pro Ile His Gly Asp 
                325                 330                 335     


Gly Asp Tyr Pro Glu Gly Met Arg Lys Lys Leu Phe Ser Val Leu Pro 
            340                 345                 350         


Ile Phe Ser Glu Ala Glu Lys His Glu Met Arg Gly Thr Ala Asp Phe 
        355                 360                 365             


Phe Ala Phe Ser Phe Gly Pro Asn Asn Phe Lys Pro Leu Asn Thr Met 
    370                 375                 380                 


Ala Lys Met Gly Gln Asn Val Ser Leu Asn Leu Arg Glu Ala Leu Asn 
385                 390                 395                 400 


Trp Ile Lys Leu Glu Tyr Asn Asn Pro Arg Ile Leu Ile Ala Glu Asn 
                405                 410                 415     


Gly Trp Phe Thr Asp Ser Arg Val Lys Thr Glu Asp Thr Thr Ala Ile 
            420                 425                 430         


Tyr Met Met Lys Asn Phe Leu Ser Gln Val Leu Gln Ala Ile Arg Leu 
        435                 440                 445             


Asp Glu Ile Arg Val Phe Gly Tyr Thr Ala Trp Ser Leu Leu Asp Gly 
    450                 455                 460                 


Phe Glu Trp Gln Asp Ala Tyr Thr Ile Arg Arg Gly Leu Phe Tyr Val 
465                 470                 475                 480 


Asp Phe Asn Ser Lys Gln Lys Glu Arg Lys Pro Lys Ser Ser Ala His 
                485                 490                 495     


Tyr Tyr Lys Gln Ile Ile Arg Glu Asn Gly Phe Ser Leu Lys Glu Ser 
            500                 505                 510         


Thr Pro Asp Val Gln Gly Gln Phe Pro Cys Asp Phe Ser Trp Gly Val 
        515                 520                 525             


Thr Glu Ser Val Leu Lys Pro Glu Ser Val Ala Ser Ser Pro Gln Phe 
    530                 535                 540                 


Ser Asp Pro His Leu Tyr Val Trp Asn Ala Thr Gly Asn Arg Leu Leu 
545                 550                 555                 560 


His Arg Val Glu Gly Val Arg Leu Lys Thr Arg Pro Ala Gln Cys Thr 
                565                 570                 575     


Asp Phe Val Asn Ile Lys Lys Gln Leu Glu Met Leu Ala Arg Met Lys 
            580                 585                 590         


Val Thr His Tyr Arg Phe Ala Leu Asp Trp Ala Ser Val Leu Pro Thr 
        595                 600                 605             


Gly Asn Leu Ser Ala Val Asn Arg Gln Ala Leu Arg Tyr Tyr Arg Cys 
    610                 615                 620                 


Val Val Ser Glu Gly Leu Lys Leu Gly Ile Ser Ala Met Val Thr Leu 
625                 630                 635                 640 


Tyr Tyr Pro Thr His Ala His Leu Gly Leu Pro Glu Pro Leu Leu His 
                645                 650                 655     


Ala Asp Gly Trp Leu Asn Pro Ser Thr Ala Glu Ala Phe Gln Ala Tyr 
            660                 665                 670         


Ala Gly Leu Cys Phe Gln Glu Leu Gly Asp Leu Val Lys Leu Trp Ile 
        675                 680                 685             


Thr Ile Asn Glu Pro Asn Arg Leu Ser Asp Ile Tyr Asn Arg Ser Gly 
    690                 695                 700                 


Asn Asp Thr Tyr Gly Ala Ala His Asn Leu Leu Val Ala His Ala Leu 
705                 710                 715                 720 


Ala Trp Arg Leu Tyr Asp Arg Gln Phe Arg Pro Ser Gln Arg Gly Ala 
                725                 730                 735     


Val Ser Leu Ser Leu His Ala Asp Trp Ala Glu Pro Ala Asn Pro Tyr 
            740                 745                 750         


Ala Asp Ser His Trp Arg Ala Ala Glu Arg Phe Leu Gln Phe Glu Ile 
        755                 760                 765             


Ala Trp Phe Ala Glu Pro Leu Phe Lys Thr Gly Asp Tyr Pro Ala Ala 
    770                 775                 780                 


Met Arg Glu Tyr Ile Ala Ser Lys His Arg Arg Gly Leu Ser Ser Ser 
785                 790                 795                 800 


Ala Leu Pro Arg Leu Thr Glu Ala Glu Arg Arg Leu Leu Lys Gly Thr 
                805                 810                 815     


Val Asp Phe Cys Ala Leu Asn His Phe Thr Thr Arg Phe Val Met His 
            820                 825                 830         


Glu Gln Leu Ala Gly Ser Arg Tyr Asp Ser Asp Arg Asp Ile Gln Phe 
        835                 840                 845             


Leu Gln Asp Ile Thr Arg Leu Ser Ser Pro Thr Arg Leu Ala Val Ile 
    850                 855                 860                 


Pro Trp Gly Val Arg Lys Leu Leu Arg Trp Val Arg Arg Asn Tyr Gly 
865                 870                 875                 880 


Asp Met Asp Ile Tyr Ile Thr Ala Ser Gly Ile Asp Asp Gln Ala Leu 
                885                 890                 895     


Glu Asp Asp Arg Leu Arg Lys Tyr Tyr Leu Gly Lys Tyr Leu Gln Glu 
            900                 905                 910         


Val Leu Lys Ala Tyr Leu Ile Asp Lys Val Arg Ile Lys Gly Tyr Tyr 
        915                 920                 925             


Ala Phe Lys Leu Ala Glu Glu Lys Ser Lys Pro Arg Phe Gly Phe Phe 
    930                 935                 940                 


Thr Ser Asp Phe Lys Ala Lys Ser Ser Ile Gln Phe Tyr Asn Lys Val 
945                 950                 955                 960 


Ile Ser Ser Arg Gly Phe Pro Phe Glu Asn Ser Ser Ser Arg Cys Ser 
                965                 970                 975     


Gln Thr Gln Glu Asn Thr Glu Cys Thr Val Cys Leu Phe Leu Val Gln 
            980                 985                 990         


Lys Lys Pro Leu Ile Phe Leu Gly  Cys Cys Phe Phe Ser  Thr Leu Val 
        995                 1000                 1005             


Leu Leu  Leu Ser Ile Ala Ile  Phe Gln Arg Gln Lys  Arg Arg Lys 
    1010                 1015                 1020             


Phe Trp  Lys Ala Lys Asn Leu  Gln His Ile Pro Leu  Lys Lys Gly 
    1025                 1030                 1035             


Lys Arg  Val Val Ser 
    1040             


<210>  38
<211>  1043
<212>  PRT
<213>  Mus musculus

<400>  38

Met Lys Thr Gly Cys Ala Ala Gly Ser Pro Gly Asn Glu Trp Ile Phe 
1               5                   10                  15      


Phe Ser Ser Asp Glu Arg Asn Thr Arg Ser Arg Lys Thr Met Ser Asn 
            20                  25                  30          


Arg Ala Leu Gln Arg Ser Ala Val Leu Ser Ala Phe Val Leu Leu Arg 
        35                  40                  45              


Ala Val Thr Gly Phe Ser Gly Asp Gly Lys Ala Ile Trp Asp Lys Lys 
    50                  55                  60                  


Gln Tyr Val Ser Pro Val Asn Pro Ser Gln Leu Phe Leu Tyr Asp Thr 
65                  70                  75                  80  


Phe Pro Lys Asn Phe Ser Trp Gly Val Gly Thr Gly Ala Phe Gln Val 
                85                  90                  95      


Glu Gly Ser Trp Lys Thr Asp Gly Arg Gly Pro Ser Ile Trp Asp Arg 
            100                 105                 110         


Tyr Val Tyr Ser His Leu Arg Gly Val Asn Gly Thr Asp Arg Ser Thr 
        115                 120                 125             


Asp Ser Tyr Ile Phe Leu Glu Lys Asp Leu Leu Ala Leu Asp Phe Leu 
    130                 135                 140                 


Gly Val Ser Phe Tyr Gln Phe Ser Ile Ser Trp Pro Arg Leu Phe Pro 
145                 150                 155                 160 


Asn Gly Thr Val Ala Ala Val Asn Ala Gln Gly Leu Arg Tyr Tyr Arg 
                165                 170                 175     


Ala Leu Leu Asp Ser Leu Val Leu Arg Asn Ile Glu Pro Ile Val Thr 
            180                 185                 190         


Leu Tyr His Trp Asp Leu Pro Leu Thr Leu Gln Glu Glu Tyr Gly Gly 
        195                 200                 205             


Trp Lys Asn Ala Thr Met Ile Asp Leu Phe Asn Asp Tyr Ala Thr Tyr 
    210                 215                 220                 


Cys Phe Gln Thr Phe Gly Asp Arg Val Lys Tyr Trp Ile Thr Ile His 
225                 230                 235                 240 


Asn Pro Tyr Leu Val Ala Trp His Gly Phe Gly Thr Gly Met His Ala 
                245                 250                 255     


Pro Gly Glu Lys Gly Asn Leu Thr Ala Val Tyr Thr Val Gly His Asn 
            260                 265                 270         


Leu Ile Lys Ala His Ser Lys Val Trp His Asn Tyr Asp Lys Asn Phe 
        275                 280                 285             


Arg Pro His Gln Lys Gly Trp Leu Ser Ile Thr Leu Gly Ser His Trp 
    290                 295                 300                 


Ile Glu Pro Asn Arg Thr Asp Asn Met Glu Asp Val Ile Asn Cys Gln 
305                 310                 315                 320 


His Ser Met Ser Ser Val Leu Gly Trp Phe Ala Asn Pro Ile His Gly 
                325                 330                 335     


Asp Gly Asp Tyr Pro Glu Phe Met Lys Thr Gly Ala Met Ile Pro Glu 
            340                 345                 350         


Phe Ser Glu Ala Glu Lys Glu Glu Val Arg Gly Thr Ala Asp Phe Phe 
        355                 360                 365             


Ala Phe Ser Phe Gly Pro Asn Asn Phe Arg Pro Ser Asn Thr Val Val 
    370                 375                 380                 


Lys Met Gly Gln Asn Val Ser Leu Asn Leu Arg Gln Val Leu Asn Trp 
385                 390                 395                 400 


Ile Lys Leu Glu Tyr Asp Asp Pro Gln Ile Leu Ile Ser Glu Asn Gly 
                405                 410                 415     


Trp Phe Thr Asp Ser Tyr Ile Lys Thr Glu Asp Thr Thr Ala Ile Tyr 
            420                 425                 430         


Met Met Lys Asn Phe Leu Asn Gln Val Leu Gln Ala Ile Lys Phe Asp 
        435                 440                 445             


Glu Ile Arg Val Phe Gly Tyr Thr Ala Trp Thr Leu Leu Asp Gly Phe 
    450                 455                 460                 


Glu Trp Gln Asp Ala Tyr Thr Thr Arg Arg Gly Leu Phe Tyr Val Asp 
465                 470                 475                 480 


Phe Asn Ser Glu Gln Lys Glu Arg Lys Pro Lys Ser Ser Ala His Tyr 
                485                 490                 495     


Tyr Lys Gln Ile Ile Gln Asp Asn Gly Phe Pro Leu Lys Glu Ser Thr 
            500                 505                 510         


Pro Asp Met Lys Gly Arg Phe Pro Cys Asp Phe Ser Trp Gly Val Thr 
        515                 520                 525             


Glu Ser Val Leu Lys Pro Glu Phe Thr Val Ser Ser Pro Gln Phe Thr 
    530                 535                 540                 


Asp Pro His Leu Tyr Val Trp Asn Val Thr Gly Asn Arg Leu Leu Tyr 
545                 550                 555                 560 


Arg Val Glu Gly Val Arg Leu Lys Thr Arg Pro Ser Gln Cys Thr Asp 
                565                 570                 575     


Tyr Val Ser Ile Lys Lys Arg Val Glu Met Leu Ala Lys Met Lys Val 
            580                 585                 590         


Thr His Tyr Gln Phe Ala Leu Asp Trp Thr Ser Ile Leu Pro Thr Gly 
        595                 600                 605             


Asn Leu Ser Lys Val Asn Arg Gln Val Leu Arg Tyr Tyr Arg Cys Val 
    610                 615                 620                 


Val Ser Glu Gly Leu Lys Leu Gly Val Phe Pro Met Val Thr Leu Tyr 
625                 630                 635                 640 


His Pro Thr His Ser His Leu Gly Leu Pro Leu Pro Leu Leu Ser Ser 
                645                 650                 655     


Gly Gly Trp Leu Asn Met Asn Thr Ala Lys Ala Phe Gln Asp Tyr Ala 
            660                 665                 670         


Glu Leu Cys Phe Arg Glu Leu Gly Asp Leu Val Lys Leu Trp Ile Thr 
        675                 680                 685             


Ile Asn Glu Pro Asn Arg Leu Ser Asp Met Tyr Asn Arg Thr Ser Asn 
    690                 695                 700                 


Asp Thr Tyr Arg Ala Ala His Asn Leu Met Ile Ala His Ala Gln Val 
705                 710                 715                 720 


Trp His Leu Tyr Asp Arg Gln Tyr Arg Pro Val Gln His Gly Ala Val 
                725                 730                 735     


Ser Leu Ser Leu His Cys Asp Trp Ala Glu Pro Ala Asn Pro Phe Val 
            740                 745                 750         


Asp Ser His Trp Lys Ala Ala Glu Arg Phe Leu Gln Phe Glu Ile Ala 
        755                 760                 765             


Trp Phe Ala Asp Pro Leu Phe Lys Thr Gly Asp Tyr Pro Ser Val Met 
    770                 775                 780                 


Lys Glu Tyr Ile Ala Ser Lys Asn Gln Arg Gly Leu Ser Ser Ser Val 
785                 790                 795                 800 


Leu Pro Arg Phe Thr Ala Lys Glu Ser Arg Leu Val Lys Gly Thr Val 
                805                 810                 815     


Asp Phe Tyr Ala Leu Asn His Phe Thr Thr Arg Phe Val Ile His Lys 
            820                 825                 830         


Gln Leu Asn Thr Asn Arg Ser Val Ala Asp Arg Asp Val Gln Phe Leu 
        835                 840                 845             


Gln Asp Ile Thr Arg Leu Ser Ser Pro Ser Arg Leu Ala Val Thr Pro 
    850                 855                 860                 


Trp Gly Val Arg Lys Leu Leu Ala Trp Ile Arg Arg Asn Tyr Arg Asp 
865                 870                 875                 880 


Arg Asp Ile Tyr Ile Thr Ala Asn Gly Ile Asp Asp Leu Ala Leu Glu 
                885                 890                 895     


Asp Asp Gln Ile Arg Lys Tyr Tyr Leu Glu Lys Tyr Val Gln Glu Ala 
            900                 905                 910         


Leu Lys Ala Tyr Leu Ile Asp Lys Val Lys Ile Lys Gly Tyr Tyr Ala 
        915                 920                 925             


Phe Lys Leu Thr Glu Glu Lys Ser Lys Pro Arg Phe Gly Phe Phe Thr 
    930                 935                 940                 


Ser Asp Phe Arg Ala Lys Ser Ser Val Gln Phe Tyr Ser Lys Leu Ile 
945                 950                 955                 960 


Ser Ser Ser Gly Leu Pro Ala Glu Asn Arg Ser Pro Ala Cys Gly Gln 
                965                 970                 975     


Pro Ala Glu Asp Thr Asp Cys Thr Ile Cys Ser Phe Leu Val Glu Lys 
            980                 985                 990         


Lys Pro Leu Ile Phe Phe Gly Cys  Cys Phe Ile Ser Thr  Leu Ala Val 
        995                 1000                 1005             


Leu Leu  Ser Ile Thr Val Phe  His His Gln Lys Arg  Arg Lys Phe 
    1010                 1015                 1020             


Gln Lys  Ala Arg Asn Leu Gln  Asn Ile Pro Leu Lys  Lys Gly His 
    1025                 1030                 1035             


Ser Arg  Val Phe Ser 
    1040             


<210>  39
<211>  63
<212>  DNA
<213>  Artificial

<220>
<223>  FGF21 secretion constructs, cloned into pVK7ara (Nde/Eco)

<400>  39
atgaaaaaaa ctgctatcgc gatcgctgta gctctggctg gtttcgcgac cgtagctaac     60

gct                                                                   63


<210>  40
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  FGF21 secretion constructs, cloned into pVK7ara (Nde/Eco)

<400>  40

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Asn Ala 
            20      


<210>  41
<211>  78
<212>  DNA
<213>  Artificial

<220>
<223>  FGF21 secretion constructs, cloned into pVK7ara (Nde/Eco)

<400>  41
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt     60

tccgcctcgg ctctcgcc                                                   78


<210>  42
<211>  26
<212>  PRT
<213>  Artificial

<220>
<223>  FGF21 secretion constructs, cloned into pVK7ara (Nde/Eco)

<400>  42

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210>  43
<211>  69
<212>  DNA
<213>  Artificial

<220>
<223>  FGF21 secretion constructs, cloned into pVK7ara (Nde/Eco)

<400>  43
atgaaaaaga atatcgcatt tcttcttgca tctatgttcg ttttttctat tgctacaaat     60

gcctatgca                                                             69


<210>  44
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  FGF21 secretion constructs, cloned into pVK7ara (Nde/Eco)

<400>  44

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


