                         SEQUENCE LISTING

<110>  University of South Florida
       The Nemours Foundation
       Mohapatra, Shyam
       Lima, John J.
 
<120>  MATERIALS AND METHODS FOR DIAGNOSIS OF ASTHMA

<130>  USF-263PTX

<150>  60/945,473
<151>  2007-06-21

<160>  18    

<170>  PatentIn version 3.3

<210>  1
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for PCR

<400>  1
cacgaagagt ctgagca                                                    17


<210>  2
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for PCR

<400>  2
ggtcaggcga aagga                                                      15


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide probe

<400>  3
ttgctatttc tatttggggg ttggattcct gg                                   32


<210>  4
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for PCR

<400>  4
agacgtaggc caagag                                                     16


<210>  5
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for PCR

<400>  5
ggagtgagca cagcat                                                     16


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide probe

<400>  6
tggacacggc attgtacatg ggat                                            24


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for PCR

<400>  7
cactcataca tgaagctgac                                                 20


<210>  8
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for PCR

<400>  8
tctacagtta gcataagatg tg                                              22


<210>  9
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide probe

<400>  9
atgtctgtgt tctctttgca gtaccgaag                                       29


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for PCR

<400>  10
cactcataca tgaagctgac                                                 20


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for PCR

<400>  11
tctacagtta gcataagatg tg                                              22


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide probe

<400>  12
acacaaatgc agcagagacc ccagg                                           25


<210>  13
<211>  840
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (95)..(547)

<400>  13
agggacagac gtaggccaag agaggggaac cagagaggaa ccagagggga gagacagagc     60

agcaagcagt ggattgctcc ttgacgacgc cagc atg agc tcc ttc tcc acc acc    115
                                      Met Ser Ser Phe Ser Thr Thr       
                                      1               5                 

acc gtg agc ttc ctc ctt tta ctg gca ttc cag ctc cta ggt cag acc      163
Thr Val Ser Phe Leu Leu Leu Leu Ala Phe Gln Leu Leu Gly Gln Thr         
        10                  15                  20                      

aga gct aat ccc atg tac aat gcc gtg tcc aac gca gac ctg atg gat      211
Arg Ala Asn Pro Met Tyr Asn Ala Val Ser Asn Ala Asp Leu Met Asp         
    25                  30                  35                          

ttc aag aat ttg ctg gac cat ttg gaa gaa aag atg cct tta gaa gat      259
Phe Lys Asn Leu Leu Asp His Leu Glu Glu Lys Met Pro Leu Glu Asp         
40                  45                  50                  55          

gag gtc gtg ccc cca caa gtg ctc agt gag ccg aat gaa gaa gcg ggg      307
Glu Val Val Pro Pro Gln Val Leu Ser Glu Pro Asn Glu Glu Ala Gly         
                60                  65                  70              

gct gct ctc agc ccc ctc cct gag gtg cct ccc tgg acc ggg gaa gtc      355
Ala Ala Leu Ser Pro Leu Pro Glu Val Pro Pro Trp Thr Gly Glu Val         
            75                  80                  85                  

agc cca gcc cag aga gat gga ggt gcc ctc ggg cgg ggc ccc tgg gac      403
Ser Pro Ala Gln Arg Asp Gly Gly Ala Leu Gly Arg Gly Pro Trp Asp         
        90                  95                  100                     

tcc tct gat cga tct gcc ctc cta aaa agc aag ctg agg gcg ctg ctc      451
Ser Ser Asp Arg Ser Ala Leu Leu Lys Ser Lys Leu Arg Ala Leu Leu         
    105                 110                 115                         

act gcc cct cgg agc ctg cgg aga tcc agc tgc ttc ggg ggc agg atg      499
Thr Ala Pro Arg Ser Leu Arg Arg Ser Ser Cys Phe Gly Gly Arg Met         
120                 125                 130                 135         

gac agg att gga gcc cag agc gga ctg ggc tgt aac agc ttc cgg tac      547
Asp Arg Ile Gly Ala Gln Ser Gly Leu Gly Cys Asn Ser Phe Arg Tyr         
                140                 145                 150             

tgaagataac agccagggag gacaagcagg gctgggccta gggacagact gcaagaggct    607

cctgtcccct ggggtctctg ctgcatttgt gtcatcttgt tgccatggag ttgtgatcat    667

cccatctaag ctgcagcttc ctgtcaacac ttctcacatc ttatgctaac tgtagataaa    727

gtggtttgat ggtgacttcc tcgcctctcc caccccatgc attaaatttt aaggtagaac    787

ctcacctgtt actgaaagtg gtttgaaagt gaataaactt cagcaccatg gac           840


<210>  14
<211>  151
<212>  PRT
<213>  Homo sapiens

<400>  14

Met Ser Ser Phe Ser Thr Thr Thr Val Ser Phe Leu Leu Leu Leu Ala 
1               5                   10                  15      


Phe Gln Leu Leu Gly Gln Thr Arg Ala Asn Pro Met Tyr Asn Ala Val 
            20                  25                  30          


Ser Asn Ala Asp Leu Met Asp Phe Lys Asn Leu Leu Asp His Leu Glu 
        35                  40                  45              


Glu Lys Met Pro Leu Glu Asp Glu Val Val Pro Pro Gln Val Leu Ser 
    50                  55                  60                  


Glu Pro Asn Glu Glu Ala Gly Ala Ala Leu Ser Pro Leu Pro Glu Val 
65                  70                  75                  80  


Pro Pro Trp Thr Gly Glu Val Ser Pro Ala Gln Arg Asp Gly Gly Ala 
                85                  90                  95      


Leu Gly Arg Gly Pro Trp Asp Ser Ser Asp Arg Ser Ala Leu Leu Lys 
            100                 105                 110         


Ser Lys Leu Arg Ala Leu Leu Thr Ala Pro Arg Ser Leu Arg Arg Ser 
        115                 120                 125             


Ser Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Leu 
    130                 135                 140                 


Gly Cys Asn Ser Phe Arg Tyr 
145                 150     


<210>  15
<211>  840
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (95)..(553)

<400>  15
agggacagac gtaggccaag agaggggaac cagagaggaa ccagagggga gagacagagc     60

agcaagcagt ggattgctcc ttgacgacgc cagc atg agc tcc ttc tcc acc acc    115
                                      Met Ser Ser Phe Ser Thr Thr       
                                      1               5                 

acc gtg agc ttc ctc ctt tta ctg gca ttc cag ctc cta ggt cag acc      163
Thr Val Ser Phe Leu Leu Leu Leu Ala Phe Gln Leu Leu Gly Gln Thr         
        10                  15                  20                      

aga gct aat ccc atg tac aat gcc gtg tcc aac gca gac ctg atg gat      211
Arg Ala Asn Pro Met Tyr Asn Ala Val Ser Asn Ala Asp Leu Met Asp         
    25                  30                  35                          

ttc aag aat ttg ctg gac cat ttg gaa gaa aag atg cct tta gaa gat      259
Phe Lys Asn Leu Leu Asp His Leu Glu Glu Lys Met Pro Leu Glu Asp         
40                  45                  50                  55          

gag gtc gtg ccc cca caa gtg ctc agt gag ccg aat gaa gaa gcg ggg      307
Glu Val Val Pro Pro Gln Val Leu Ser Glu Pro Asn Glu Glu Ala Gly         
                60                  65                  70              

gct gct ctc agc ccc ctc cct gag gtg cct ccc tgg acc ggg gaa gtc      355
Ala Ala Leu Ser Pro Leu Pro Glu Val Pro Pro Trp Thr Gly Glu Val         
            75                  80                  85                  

agc cca gcc cag aga gat gga ggt gcc ctc ggg cgg ggc ccc tgg gac      403
Ser Pro Ala Gln Arg Asp Gly Gly Ala Leu Gly Arg Gly Pro Trp Asp         
        90                  95                  100                     

tcc tct gat cga tct gcc ctc cta aaa agc aag ctg agg gcg ctg ctc      451
Ser Ser Asp Arg Ser Ala Leu Leu Lys Ser Lys Leu Arg Ala Leu Leu         
    105                 110                 115                         

act gcc cct cgg agc ctg cgg aga tcc agc tgc ttc ggg ggc agg atg      499
Thr Ala Pro Arg Ser Leu Arg Arg Ser Ser Cys Phe Gly Gly Arg Met         
120                 125                 130                 135         

gac agg att gga gcc cag agc gga ctg ggc tgt aac agc ttc cgg tac      547
Asp Arg Ile Gly Ala Gln Ser Gly Leu Gly Cys Asn Ser Phe Arg Tyr         
                140                 145                 150             

cga aga taacagccag ggaggacaag cagggctggg cctagggaca gactgcaaga       603
Arg Arg                                                                 
                                                                        

ggctcctgtc ccctggggtc tctgctgcat ttgtgtcatc ttgttgccat ggagttgtga    663

tcatcccatc taagctgcag cttcctgtca acacttctca catcttatgc taactgtaga    723

taaagtggtt tgatggtgac ttcctcgcct ctcccacccc atgcattaaa ttttaaggta    783

gaacctcacc tgttactgaa agtggtttga aagtgaataa acttcagcac catggac       840


<210>  16
<211>  153
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Ser Ser Phe Ser Thr Thr Thr Val Ser Phe Leu Leu Leu Leu Ala 
1               5                   10                  15      


Phe Gln Leu Leu Gly Gln Thr Arg Ala Asn Pro Met Tyr Asn Ala Val 
            20                  25                  30          


Ser Asn Ala Asp Leu Met Asp Phe Lys Asn Leu Leu Asp His Leu Glu 
        35                  40                  45              


Glu Lys Met Pro Leu Glu Asp Glu Val Val Pro Pro Gln Val Leu Ser 
    50                  55                  60                  


Glu Pro Asn Glu Glu Ala Gly Ala Ala Leu Ser Pro Leu Pro Glu Val 
65                  70                  75                  80  


Pro Pro Trp Thr Gly Glu Val Ser Pro Ala Gln Arg Asp Gly Gly Ala 
                85                  90                  95      


Leu Gly Arg Gly Pro Trp Asp Ser Ser Asp Arg Ser Ala Leu Leu Lys 
            100                 105                 110         


Ser Lys Leu Arg Ala Leu Leu Thr Ala Pro Arg Ser Leu Arg Arg Ser 
        115                 120                 125             


Ser Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Leu 
    130                 135                 140                 


Gly Cys Asn Ser Phe Arg Tyr Arg Arg 
145                 150             


<210>  17
<211>  840
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (95)..(547)

<400>  17
agggacagac gtaggccaag agaggggaac cagagaggaa ccagagggga gagacagagc     60

agcaagcagt ggattgctcc ttgacgacgc cagc atg agc tcc ttc tcc acc acc    115
                                      Met Ser Ser Phe Ser Thr Thr       
                                      1               5                 

acc gtg agc ttc ctc ctt tta ctg gca ttc cag ctc cta ggt cag acc      163
Thr Val Ser Phe Leu Leu Leu Leu Ala Phe Gln Leu Leu Gly Gln Thr         
        10                  15                  20                      

aga gct aat ccc atg tac aat gcc atg tcc aac gca gac ctg atg gat      211
Arg Ala Asn Pro Met Tyr Asn Ala Met Ser Asn Ala Asp Leu Met Asp         
    25                  30                  35                          

ttc aag aat ttg ctg gac cat ttg gaa gaa aag atg cct tta gaa gat      259
Phe Lys Asn Leu Leu Asp His Leu Glu Glu Lys Met Pro Leu Glu Asp         
40                  45                  50                  55          

gag gtc gtg ccc cca caa gtg ctc agt gag ccg aat gaa gaa gcg ggg      307
Glu Val Val Pro Pro Gln Val Leu Ser Glu Pro Asn Glu Glu Ala Gly         
                60                  65                  70              

gct gct ctc agc ccc ctc cct gag gtg cct ccc tgg acc ggg gaa gtc      355
Ala Ala Leu Ser Pro Leu Pro Glu Val Pro Pro Trp Thr Gly Glu Val         
            75                  80                  85                  

agc cca gcc cag aga gat gga ggt gcc ctc ggg cgg ggc ccc tgg gac      403
Ser Pro Ala Gln Arg Asp Gly Gly Ala Leu Gly Arg Gly Pro Trp Asp         
        90                  95                  100                     

tcc tct gat cga tct gcc ctc cta aaa agc aag ctg agg gcg ctg ctc      451
Ser Ser Asp Arg Ser Ala Leu Leu Lys Ser Lys Leu Arg Ala Leu Leu         
    105                 110                 115                         

act gcc cct cgg agc ctg cgg aga tcc agc tgc ttc ggg ggc agg atg      499
Thr Ala Pro Arg Ser Leu Arg Arg Ser Ser Cys Phe Gly Gly Arg Met         
120                 125                 130                 135         

gac agg att gga gcc cag agc gga ctg ggc tgt aac agc ttc cgg tac      547
Asp Arg Ile Gly Ala Gln Ser Gly Leu Gly Cys Asn Ser Phe Arg Tyr         
                140                 145                 150             

tgaagataac agccagggag gacaagcagg gctgggccta gggacagact gcaagaggct    607

cctgtcccct ggggtctctg ctgcatttgt gtcatcttgt tgccatggag ttgtgatcat    667

cccatctaag ctgcagcttc ctgtcaacac ttctcacatc ttatgctaac tgtagataaa    727

gtggtttgat ggtgacttcc tcgcctctcc caccccatgc attaaatttt aaggtagaac    787

ctcacctgtt actgaaagtg gtttgaaagt gaataaactt cagcaccatg gac           840


<210>  18
<211>  151
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Ser Ser Phe Ser Thr Thr Thr Val Ser Phe Leu Leu Leu Leu Ala 
1               5                   10                  15      


Phe Gln Leu Leu Gly Gln Thr Arg Ala Asn Pro Met Tyr Asn Ala Met 
            20                  25                  30          


Ser Asn Ala Asp Leu Met Asp Phe Lys Asn Leu Leu Asp His Leu Glu 
        35                  40                  45              


Glu Lys Met Pro Leu Glu Asp Glu Val Val Pro Pro Gln Val Leu Ser 
    50                  55                  60                  


Glu Pro Asn Glu Glu Ala Gly Ala Ala Leu Ser Pro Leu Pro Glu Val 
65                  70                  75                  80  


Pro Pro Trp Thr Gly Glu Val Ser Pro Ala Gln Arg Asp Gly Gly Ala 
                85                  90                  95      


Leu Gly Arg Gly Pro Trp Asp Ser Ser Asp Arg Ser Ala Leu Leu Lys 
            100                 105                 110         


Ser Lys Leu Arg Ala Leu Leu Thr Ala Pro Arg Ser Leu Arg Arg Ser 
        115                 120                 125             


Ser Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Leu 
    130                 135                 140                 


Gly Cys Asn Ser Phe Arg Tyr 
145                 150     


