
                                SEQUENCE LISTING

<110> CHOI, Byung-Kwon
      BOBROWICZ, Piotr
      COOK,  W. James

<120> VECTORS AND YEAST STRAINS FOR PROTEIN
  PRODUCTION

<130> MRL-BIO-22395

<150> 61/0066,409         
<151> 2008-02-20  

<150> 61/188,723          
<151> 2008-08-12  

<160> 77

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer hPDI/UP1

<400> 1
agcgctgacg cccccgagga ggaggaccac                                  30

<210> 2
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer hPDI/LP-PacI

<400> 2
ccttaattaa ttacagttca tcatgcacag ctttctgatc at                    42

<210> 3
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PB248

<400> 3
atgaattcag gccatatcgg ccattgttta ctgtgcgccc acagtag               47

<210> 4
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PB249

<400> 4
atgtttaaac gtgaggatta ctggtgatga aagac                            35

<210> 5
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PB250

<400> 5
agactagtct atttggagac attgacggat ccac                             34

<210> 6
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PB251

<400> 6
atctcgagag gccatgcagg ccaaccacaa gatgaatcaa attttg                46

<210> 7
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PpPDI/UPi-1

<400> 7
ggtgaggttg aggtcccaag tgactatcaa ggtc                             34

<210> 8
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PpPDI/LPi-1

<400> 8
gaccttgata gtcacttggg acctcaacct cacc                             34

<210> 9
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PpPDI/UPi-2

<400> 9
cgccaatgat gaggatgcct cttcaaaggt tgtg                             34

<210> 10
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PpPDI/LPi-2

<400> 10
cacaaccttt gaagaggcat cctcatcatt ggcg                             34

<210> 11
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PpPDI-5'/UP

<400> 11
ggcgattgca ttcgcgactg tatc                                        24

<210> 12
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer hPDI-3'/LP

<400> 12
cctagagagc ggtggccaag atg                                         23

<210> 13
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer hPDI/UP

<400> 13
gtggccacac cagggggcat ggaac                                       25

<210> 14
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer hPDI-3'/LP

<400> 14
cctagagagc ggtggccaag atg                                         23

<210> 15
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer hGRP94/UP1

<400> 15
agcgctgacg atgaagttga tgtggatggt acagtag                          37

<210> 16
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer hGRP94/LP1

<400> 16
ggccggcctt acaattcatc atgttcagct gtagattc                         38

<210> 17
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PMT1-KO1

<400> 17
tgaacccatc tgtaaataga atgc                                        24

<210> 18
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PMT1-KO2

<400> 18
gtgtcaccta aatcgtatgt gcccatttac tggaagctgc taacc                 45

<210> 19
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PMT1-KO3

<400> 19
ctccctatag tgagtcgtat tcatcattgt actttggtat attgg                 45

<210> 20
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PMT1-KO4

<400> 20
tatttgtacc tgcgtcctgt ttgc                                        24

<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PR29

<400> 21
cacatacgat ttaggtgaca c                                           21

<210> 22
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PR32

<400> 22
aatacgactc actataggga g                                           21

<210> 23
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PMT4-KO1

<400> 23
tgctctccgc gtgcaataga aact                                        24

<210> 24
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PMT4-KO2

<400> 24
ctccctatag tgagtcgtat tcacagtgta ccatctttca tctcc                 45

<210> 25
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PMT4-KO3

<400> 25
gtgtcaccta aatcgtatgt gaacctaact ctaattcttc aaagc                 45

<210> 26
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer PMT4-KO4

<400> 26
actagggtat ataattccca aggt                                        24

<210> 27
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> Saccharomyces cerevisiae mating factor pre-signal
      peptide

<400> 27
atgagattcc catccatctt cactgctgtt ttgttcgctg cttcttctgc tttggct    57

<210> 28
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> Saccharomyces cerevisiae mating factor pre-signal
      peptide

<400> 28
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
 1               5                  10                  15      
Ala Leu Ala
            


<210> 29
<211> 1353
<212> DNA
<213> Artificial Sequence

<220> 
<223> Anti-Her2 Heavy chain (VH + IgG1 constant region)

<400> 29
gaggttcagt tggttgaatc tggaggagga ttggttcaac ctggtggttc tttgagattg 60
tcctgtgctg cttccggttt caacatcaag gacacttaca tccactgggt tagacaagct 120
ccaggaaagg gattggagtg ggttgctaga atctacccaa ctaacggtta cacaagatac 180
gctgactccg ttaagggaag attcactatc tctgctgaca cttccaagaa cactgcttac 240
ttgcagatga actccttgag agctgaggat actgctgttt actactgttc cagatggggt 300
ggtgatggtt tctacgctat ggactactgg ggtcaaggaa ctttggttac tgtttcctcc 360
gcttctacta agggaccatc tgttttccca ttggctccat cttctaagtc tacttccggt 420
ggtactgctg ctttgggatg tttggttaaa gactacttcc cagagccagt tactgtttct 480
tggaactccg gtgctttgac ttctggtgtt cacactttcc cagctgtttt gcaatcttcc 540
ggtttgtact ctttgtcctc cgttgttact gttccatcct cttccttggg tactcagact 600
tacatctgta acgttaacca caagccatcc aacactaagg ttgacaagaa ggttgagcca 660
aagtcctgtg acaagactca tacttgtcca ccatgtccag ctccagaatt gttgggtggt 720
ccttccgttt ttttgttccc accaaagcca aaggacactt tgatgatctc cagaactcca 780
gaggttacat gtgttgttgt tgacgtttct cacgaggacc cagaggttaa gttcaactgg 840
tacgttgacg gtgttgaagt tcacaacgct aagactaagc caagagagga gcagtacaac 900
tccacttaca gagttgtttc cgttttgact gttttgcacc aggattggtt gaacggaaag 960
gagtacaagt gtaaggtttc caacaaggct ttgccagctc caatcgaaaa gactatctcc 1020
aaggctaagg gtcaaccaag agagccacag gtttacactt tgccaccatc cagagatgag 1080
ttgactaaga accaggtttc cttgacttgt ttggttaagg gattctaccc atccgacatt 1140
gctgttgaat gggagtctaa cggtcaacca gagaacaact acaagactac tccacctgtt 1200
ttggactctg acggttcctt tttcttgtac tccaagttga ctgttgacaa gtccagatgg 1260
caacagggta acgttttctc ctgttccgtt atgcatgagg ctttgcacaa ccactacact 1320
caaaagtcct tgtctttgtc ccctggtaag taa                              1353

<210> 30
<211> 450
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-Her2 Heavy chain (VH + IgG1 constant region)

<400> 30
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr
            20                  25                  30          
Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln
            100                 105                 110         
Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val
        115                 120                 125             
Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala
    130                 135                 140                 
Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser
145                 150                 155                 160 
Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val
                165                 170                 175     
Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro
            180                 185                 190         
Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys
        195                 200                 205             
Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp
    210                 215                 220                 
Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly
225                 230                 235                 240 
Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
                245                 250                 255     
Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu
            260                 265                 270         
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
        275                 280                 285             
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg
    290                 295                 300                 
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
305                 310                 315                 320 
Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu
                325                 330                 335     
Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
            340                 345                 350         
Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
        355                 360                 365             
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
    370                 375                 380                 
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
385                 390                 395                 400 
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp
                405                 410                 415     
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His
            420                 425                 430         
Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
        435                 440                 445             
Gly Lys
    450 


<210> 31
<211> 645
<212> DNA
<213> Artificial Sequence

<220> 
<223> Anti-Her2 light chain (VL + Kappa constant region)

<400> 31
gacatccaaa tgactcaatc cccatcttct ttgtctgctt ccgttggtga cagagttact 60
atcacttgta gagcttccca ggacgttaat actgctgttg cttggtatca acagaagcca 120
ggaaaggctc caaagttgtt gatctactcc gcttccttct tgtactctgg tgttccatcc 180
agattctctg gttccagatc cggtactgac ttcactttga ctatctcctc cttgcaacca 240
gaagatttcg ctacttacta ctgtcagcag cactacacta ctccaccaac tttcggacag 300
ggtactaagg ttgagatcaa gagaactgtt gctgctccat ccgttttcat tttcccacca 360
tccgacgaac agttgaagtc tggtacagct tccgttgttt gtttgttgaa caacttctac 420
ccaagagagg ctaaggttca gtggaaggtt gacaacgctt tgcaatccgg taactcccaa 480
gaatccgtta ctgagcaaga ctctaaggac tccacttact ccttgtcctc cactttgact 540
ttgtccaagg ctgattacga gaagcacaag gtttacgctt gtgaggttac acatcagggt 600
ttgtcctccc cagttactaa gtccttcaac agaggagagt gttaa                 645

<210> 32
<211> 213
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-Her2 light chain (VL + Kappa constant region)

<400> 32
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala
            100                 105                 110         
Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly
        115                 120                 125             
Thr Ala Ser Val Val Cys Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys
    130                 135                 140                 
Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu
145                 150                 155                 160 
Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser
                165                 170                 175     
Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala
            180                 185                 190         
Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe
        195                 200                 205             
Asn Arg Gly Glu Cys
    210             


<210> 33
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Alpha amylase signal peptide (from Aspergillus
      niger  -amylase)

<400> 33
atggttgctt ggtggtcctt gttcttgtac ggattgcaag ttgctgctcc agctttggct 60


<210> 34
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> Alpha amylase signal peptide (from Aspergillus
      niger  -amylase)

<400> 34
Met Val Ala Trp Trp Ser Leu Phe Leu Tyr Gly Leu Gln Val Ala Ala
 1               5                  10                  15      
Pro Ala Leu Ala
            20  


<210> 35
<211> 397
<212> DNA
<213> Artificial Sequence

<220> 
<223> Anti-CD20 Light chain Variable Region

<400> 35
gagatcgttt tgacacagtc cccagctact ttgtctttgt ccccaggtga aagagctaca 60
ttgtcctgta gagcttccca atctgtttcc tcctacttgg cttggtatca acaaaagcca 120
ggacaggctc caagattgtt gatctacgac gcttccaata gagctactgg tatcccagct 180
agattctctg gttctggttc cggtactgac ttcactttga ctatctcttc cttggaacca 240
gaggacttcg ctgtttacta ctgtcagcag agatccaatt ggccattgac tttcggtggt 300
ggtactaagg ttgagatcaa gcgtacggtt gctgctcctt ccgttttcat tttcccacca 360
tccgacgaac aattgaagtc tggtacccaa ttcgccc                          397

<210> 36
<211> 132
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-CD20 Light chain Variable Region

<400> 36
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly
 1               5                  10                  15      
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
        35                  40                  45              
Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro
65                  70                  75                  80  
Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro Leu
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala
            100                 105                 110         
Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly
        115                 120                 125             
Thr Gln Phe Ala
    130         


<210> 37
<211> 445
<212> DNA
<213> Artificial Sequence

<220> 
<223> Anti-CD20 Heavy chain Variable Region

<400> 37
gctgttcagc tggttgaatc tggtggtgga ttggttcaac ctggtagatc cttgagattg 60
tcctgtgctg cttccggttt tactttcggt gactacacta tgcactgggt tagacaagct 120
ccaggaaagg gattggaatg ggtttccggt atttcttgga actccggttc cattggttac 180
gctgattccg ttaagggaag attcactatc tccagagaca acgctaagaa ctccttgtac 240
ttgcagatga actccttgag agctgaggat actgctttgt actactgtac taaggacaac 300
caatacggtt ctggttccac ttacggattg ggagtttggg gacagggaac tttggttact 360
gtctcgagtg cttctactaa gggaccatcc gtttttccat tggctccatc ctctaagtct 420
acttccggtg gtacccaatt cgccc                                       445

<210> 38
<211> 148
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-CD20 Heavy chain Variable Region

<400> 38
Ala Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Gly Asp Tyr
            20                  25                  30          
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile Gly Tyr Ala Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr Tyr Cys
                85                  90                  95      
Thr Lys Asp Asn Gln Tyr Gly Ser Gly Ser Thr Tyr Gly Leu Gly Val
            100                 105                 110         
Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly
        115                 120                 125             
Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly
    130                 135                 140                 
Thr Gln Phe Ala
145             


<210> 39
<211> 1476
<212> DNA
<213> Artificial Sequence

<220> 
<223> human PDI without leader

<400> 39
gacgcccccg aggaggagga ccacgtcttg gtgctgcgga aaagcaactt cgcggaggcg 60
ctggcggccc acaagtaccc gccggtggag ttccatgccc cctggtgtgg ccactgcaag 120
gctctggccc ctgagtatgc caaagccgct gggaagctga aggcagaagg ttccgagatc 180
aggttggcca aggtggacgc cacggaggag tctgacctag cccagcagta cggcgtgcgc 240
ggctatccca ccatcaagtt cttcaggaat ggagacacgg cttcccccaa ggaatataca 300
gctggcagag aggctgatga catcgtgaac tggctgaaga agcgcacggg cccggctgcc 360
accaccctgc ctgacggcgc agctgcagag tccttggtgg agtccagcga ggtggccgtc 420
atcggcttct tcaaggacgt ggagtcggac tctgccaagc agtttttgca ggcagcagag 480
gccatcgatg acataccatt tgggatcact tccaacagtg acgtgttctc caaataccag 540
ctcgacaaag atggggttgt cctctttaag aagtttgatg aaggccggaa caactttgaa 600
ggggaggtca ccaaggagaa cctgctggac tttatcaaac acaaccagct gccccttgtc 660
atcgagttca ccgagcagac agccccgaag atttttggag gtgaaatcaa gactcacatc 720
ctgctgttct tgcccaagag tgtgtctgac tatgacggca aactgagcaa cttcaaaaca 780
gcagccgaga gcttcaaggg caagatcctg ttcatcttca tcgacagcga ccacaccgac 840
aaccagcgca tcctcgagtt ctttggcctg aagaaggaag agtgcccggc cgtgcgcctc 900
atcaccttgg aggaggagat gaccaagtac aagcccgaat cggaggagct gacggcagag 960
aggatcacag agttctgcca ccgcttcctg gagggcaaaa tcaagcccca cctgatgagc 1020
caggagctgc cggaggactg ggacaagcag cctgtcaagg tgcttgttgg gaagaacttt 1080
gaagacgtgg cttttgatga gaaaaaaaac gtctttgtgg agttctatgc cccatggtgt 1140
ggtcactgca aacagttggc tcccatttgg gataaactgg gagagacgta caaggaccat 1200
gagaacatcg tcatcgccaa gatggactcg actgccaacg aggtggaggc cgtcaaagtg 1260
cacggcttcc ccacactcgg gttctttcct gccagtgccg acaggacggt cattgattac 1320
aacggggaac gcacgctgga tggttttaag aaattcctag agagcggtgg ccaagatggg 1380
gcaggggatg ttgacgacct cgaggacctc gaagaagcag aggagccaga catggaggaa 1440
gacgatgacc agaaagctgt gaaagatgaa ctgtaa                           1476

<210> 40
<211> 491
<212> PRT
<213> Artificial Sequence

<220> 
<223> human PDI without leader

<400> 40
Asp Ala Pro Glu Glu Glu Asp His Val Leu Val Leu Arg Lys Ser Asn
 1               5                  10                  15      
Phe Ala Glu Ala Leu Ala Ala His Lys Tyr Pro Pro Val Glu Phe His
            20                  25                  30          
Ala Pro Trp Cys Gly His Cys Lys Ala Leu Ala Pro Glu Tyr Ala Lys
        35                  40                  45              
Ala Ala Gly Lys Leu Lys Ala Glu Gly Ser Glu Ile Arg Leu Ala Lys
    50                  55                  60                  
Val Asp Ala Thr Glu Glu Ser Asp Leu Ala Gln Gln Tyr Gly Val Arg
65                  70                  75                  80  
Gly Tyr Pro Thr Ile Lys Phe Phe Arg Asn Gly Asp Thr Ala Ser Pro
                85                  90                  95      
Lys Glu Tyr Thr Ala Gly Arg Glu Ala Asp Asp Ile Val Asn Trp Leu
            100                 105                 110         
Lys Lys Arg Thr Gly Pro Ala Ala Thr Thr Leu Pro Asp Gly Ala Ala
        115                 120                 125             
Ala Glu Ser Leu Val Glu Ser Ser Glu Val Ala Val Ile Gly Phe Phe
    130                 135                 140                 
Lys Asp Val Glu Ser Asp Ser Ala Lys Gln Phe Leu Gln Ala Ala Glu
145                 150                 155                 160 
Ala Ile Asp Asp Ile Pro Phe Gly Ile Thr Ser Asn Ser Asp Val Phe
                165                 170                 175     
Ser Lys Tyr Gln Leu Asp Lys Asp Gly Val Val Leu Phe Lys Lys Phe
            180                 185                 190         
Asp Glu Gly Arg Asn Asn Phe Glu Gly Glu Val Thr Lys Glu Asn Leu
        195                 200                 205             
Leu Asp Phe Ile Lys His Asn Gln Leu Pro Leu Val Ile Glu Phe Thr
    210                 215                 220                 
Glu Gln Thr Ala Pro Lys Ile Phe Gly Gly Glu Ile Lys Thr His Ile
225                 230                 235                 240 
Leu Leu Phe Leu Pro Lys Ser Val Ser Asp Tyr Asp Gly Lys Leu Ser
                245                 250                 255     
Asn Phe Lys Thr Ala Ala Glu Ser Phe Lys Gly Lys Ile Leu Phe Ile
            260                 265                 270         
Phe Ile Asp Ser Asp His Thr Asp Asn Gln Arg Ile Leu Glu Phe Phe
        275                 280                 285             
Gly Leu Lys Lys Glu Glu Cys Pro Ala Val Arg Leu Ile Thr Leu Glu
    290                 295                 300                 
Glu Glu Met Thr Lys Tyr Lys Pro Glu Ser Glu Glu Leu Thr Ala Glu
305                 310                 315                 320 
Arg Ile Thr Glu Phe Cys His Arg Phe Leu Glu Gly Lys Ile Lys Pro
                325                 330                 335     
His Leu Met Ser Gln Glu Leu Pro Glu Asp Trp Asp Lys Gln Pro Val
            340                 345                 350         
Lys Val Leu Val Gly Lys Asn Phe Glu Asp Val Ala Phe Asp Glu Lys
        355                 360                 365             
Lys Asn Val Phe Val Glu Phe Tyr Ala Pro Trp Cys Gly His Cys Lys
    370                 375                 380                 
Gln Leu Ala Pro Ile Trp Asp Lys Leu Gly Glu Thr Tyr Lys Asp His
385                 390                 395                 400 
Glu Asn Ile Val Ile Ala Lys Met Asp Ser Thr Ala Asn Glu Val Glu
                405                 410                 415     
Ala Val Lys Val His Gly Phe Pro Thr Leu Gly Phe Phe Pro Ala Ser
            420                 425                 430         
Ala Asp Arg Thr Val Ile Asp Tyr Asn Gly Glu Arg Thr Leu Asp Gly
        435                 440                 445             
Phe Lys Lys Phe Leu Glu Ser Gly Gly Gln Asp Gly Ala Gly Asp Val
    450                 455                 460                 
Asp Asp Leu Glu Asp Leu Glu Glu Ala Glu Glu Pro Asp Met Glu Glu
465                 470                 475                 480 
Asp Asp Asp Gln Lys Ala Val His Asp Glu Leu
                485                 490     


<210> 41
<211> 1554
<212> DNA
<213> Pichia pastoris

<220> 
<223> Pichia pastoris PDI1 Gene

<400> 41
atgcaattca actggaatat taaaactgtg gcaagtattt tgtccgctct cacactagca 60
caagcaagtg atcaggaggc tattgctcca gaggactctc atgtcgtcaa attgactgaa 120
gccacttttg agtctttcat caccagtaat cctcacgttt tggcagagtt ttttgcccct 180
tggtgtggtc actgtaagaa gttgggccct gaacttgttt ctgctgccga gatcttaaag 240
gacaatgagc aggttaagat tgctcaaatt gattgtacgg aggagaagga attatgtcaa 300
ggctacgaaa ttaaagggta tcctactttg aaggtgttcc atggtgaggt tgaggtccca 360
agtgactatc aaggtcaaag acagagccaa agcattgtca gctatatgct aaagcagagt 420
ttaccccctg tcagtgaaat caatgcaacc aaagatttag acgacacaat cgccgaggca 480
aaagagcccg tgattgtgca agtactaccg gaagatgcat ccaacttgga atctaacacc 540
acattttacg gagttgccgg tactctcaga gagaaattca cttttgtctc cactaagtct 600
actgattatg ccaaaaaata cactagcgac tcgactcctg cctatttgct tgtcagacct 660
ggcgaggaac ctagtgttta ctctggtgag gagttagatg agactcattt ggtgcactgg 720
attgatattg agtccaaacc tctatttgga gacattgacg gatccacctt caaatcatat 780
gctgaagcta acatcccttt agcctactat ttctatgaga acgaagaaca acgtgctgct 840
gctgccgata ttattaaacc ttttgctaaa gagcaacgtg gcaaaattaa ctttgttggc 900
ttagatgccg ttaaattcgg taagcatgcc aagaacttaa acatggatga agagaaactc 960
cctctatttg tcattcatga tttggtgagc aacaagaagt ttggagttcc tcaagaccaa 1020
gaattgacga acaaagatgt gaccgagctg attgagaaat tcatcgcagg agaggcagaa 1080
ccaattgtga aatcagagcc aattccagaa attcaagaag agaaagtctt caagctagtc 1140
ggaaaggccc acgatgaagt tgtcttcgat gaatctaaag atgttctagt caagtactac 1200
gccccttggt gtggtcactg taagagaatg gctcctgctt atgaggaatt ggctactctt 1260
tacgccaatg atgaggatgc ctcttcaaag gttgtgattg caaaacttga tcacactttg 1320
aacgatgtcg acaacgttga tattcaaggt tatcctactt tgatccttta tccagctggt 1380
gataaatcca atcctcaact gtatgatgga tctcgtgacc tagaatcatt ggctgagttt 1440
gtaaaggaga gaggaaccca caaagtggat gccctagcac tcagaccagt cgaggaagaa 1500
aaggaagctg aagaagaagc tgaaagtgag gcagacgctc acgacgagct ttaa       1554

<210> 42
<211> 1554
<212> PRT
<213> Pichia pastoris

<400> 42
Ala Thr Gly Cys Ala Ala Thr Thr Cys Ala Ala Cys Thr Gly Gly Ala
 1               5                  10                  15      
Ala Thr Ala Thr Thr Ala Ala Ala Ala Cys Thr Gly Thr Gly Gly Cys
            20                  25                  30          
Ala Ala Gly Thr Ala Thr Thr Thr Thr Gly Thr Cys Cys Gly Cys Thr
        35                  40                  45              
Cys Thr Cys Ala Cys Ala Cys Thr Ala Gly Cys Ala Cys Ala Ala Gly
    50                  55                  60                  
Cys Ala Ala Gly Thr Gly Ala Thr Cys Ala Gly Gly Ala Gly Gly Cys
65                  70                  75                  80  
Thr Ala Thr Thr Gly Cys Thr Cys Cys Ala Gly Ala Gly Gly Ala Cys
                85                  90                  95      
Thr Cys Thr Cys Ala Thr Gly Thr Cys Gly Thr Cys Ala Ala Ala Thr
            100                 105                 110         
Thr Gly Ala Cys Thr Gly Ala Ala Gly Cys Cys Ala Cys Thr Thr Thr
        115                 120                 125             
Thr Gly Ala Gly Thr Cys Thr Thr Thr Cys Ala Thr Cys Ala Cys Cys
    130                 135                 140                 
Ala Gly Thr Ala Ala Thr Cys Cys Thr Cys Ala Cys Gly Thr Thr Thr
145                 150                 155                 160 
Thr Gly Gly Cys Ala Gly Ala Gly Thr Thr Thr Thr Thr Thr Gly Cys
                165                 170                 175     
Cys Cys Cys Thr Thr Gly Gly Thr Gly Thr Gly Gly Thr Cys Ala Cys
            180                 185                 190         
Thr Gly Thr Ala Ala Gly Ala Ala Gly Thr Thr Gly Gly Gly Cys Cys
        195                 200                 205             
Cys Thr Gly Ala Ala Cys Thr Thr Gly Thr Thr Thr Cys Thr Gly Cys
    210                 215                 220                 
Thr Gly Cys Cys Gly Ala Gly Ala Thr Cys Thr Thr Ala Ala Ala Gly
225                 230                 235                 240 
Gly Ala Cys Ala Ala Thr Gly Ala Gly Cys Ala Gly Gly Thr Thr Ala
                245                 250                 255     
Ala Gly Ala Thr Thr Gly Cys Thr Cys Ala Ala Ala Thr Thr Gly Ala
            260                 265                 270         
Thr Thr Gly Thr Ala Cys Gly Gly Ala Gly Gly Ala Gly Ala Ala Gly
        275                 280                 285             
Gly Ala Ala Thr Thr Ala Thr Gly Thr Cys Ala Ala Gly Gly Cys Thr
    290                 295                 300                 
Ala Cys Gly Ala Ala Ala Thr Thr Ala Ala Ala Gly Gly Gly Thr Ala
305                 310                 315                 320 
Thr Cys Cys Thr Ala Cys Thr Thr Thr Gly Ala Ala Gly Gly Thr Gly
                325                 330                 335     
Thr Thr Cys Cys Ala Thr Gly Gly Thr Gly Ala Gly Gly Thr Thr Gly
            340                 345                 350         
Ala Gly Gly Thr Cys Cys Cys Ala Ala Gly Thr Gly Ala Cys Thr Ala
        355                 360                 365             
Thr Cys Ala Ala Gly Gly Thr Cys Ala Ala Ala Gly Ala Cys Ala Gly
    370                 375                 380                 
Ala Gly Cys Cys Ala Ala Ala Gly Cys Ala Thr Thr Gly Thr Cys Ala
385                 390                 395                 400 
Gly Cys Thr Ala Thr Ala Thr Gly Cys Thr Ala Ala Ala Gly Cys Ala
                405                 410                 415     
Gly Ala Gly Thr Thr Thr Ala Cys Cys Cys Cys Cys Thr Gly Thr Cys
            420                 425                 430         
Ala Gly Thr Gly Ala Ala Ala Thr Cys Ala Ala Thr Gly Cys Ala Ala
        435                 440                 445             
Cys Cys Ala Ala Ala Gly Ala Thr Thr Thr Ala Gly Ala Cys Gly Ala
    450                 455                 460                 
Cys Ala Cys Ala Ala Thr Cys Gly Cys Cys Gly Ala Gly Gly Cys Ala
465                 470                 475                 480 
Ala Ala Ala Gly Ala Gly Cys Cys Cys Gly Thr Gly Ala Thr Thr Gly
                485                 490                 495     
Thr Gly Cys Ala Ala Gly Thr Ala Cys Thr Ala Cys Cys Gly Gly Ala
            500                 505                 510         
Ala Gly Ala Thr Gly Cys Ala Thr Cys Cys Ala Ala Cys Thr Thr Gly
        515                 520                 525             
Gly Ala Ala Thr Cys Thr Ala Ala Cys Ala Cys Cys Ala Cys Ala Thr
    530                 535                 540                 
Thr Thr Thr Ala Cys Gly Gly Ala Gly Thr Thr Gly Cys Cys Gly Gly
545                 550                 555                 560 
Thr Ala Cys Thr Cys Thr Cys Ala Gly Ala Gly Ala Gly Ala Ala Ala
                565                 570                 575     
Thr Thr Cys Ala Cys Thr Thr Thr Thr Gly Thr Cys Thr Cys Cys Ala
            580                 585                 590         
Cys Thr Ala Ala Gly Thr Cys Thr Ala Cys Thr Gly Ala Thr Thr Ala
        595                 600                 605             
Thr Gly Cys Cys Ala Ala Ala Ala Ala Ala Thr Ala Cys Ala Cys Thr
    610                 615                 620                 
Ala Gly Cys Gly Ala Cys Thr Cys Gly Ala Cys Thr Cys Cys Thr Gly
625                 630                 635                 640 
Cys Cys Thr Ala Thr Thr Thr Gly Cys Thr Thr Gly Thr Cys Ala Gly
                645                 650                 655     
Ala Cys Cys Thr Gly Gly Cys Gly Ala Gly Gly Ala Ala Cys Cys Thr
            660                 665                 670         
Ala Gly Thr Gly Thr Thr Thr Ala Cys Thr Cys Thr Gly Gly Thr Gly
        675                 680                 685             
Ala Gly Gly Ala Gly Thr Thr Ala Gly Ala Thr Gly Ala Gly Ala Cys
    690                 695                 700                 
Thr Cys Ala Thr Thr Thr Gly Gly Thr Gly Cys Ala Cys Thr Gly Gly
705                 710                 715                 720 
Ala Thr Thr Gly Ala Thr Ala Thr Thr Gly Ala Gly Thr Cys Cys Ala
                725                 730                 735     
Ala Ala Cys Cys Thr Cys Thr Ala Thr Thr Thr Gly Gly Ala Gly Ala
            740                 745                 750         
Cys Ala Thr Thr Gly Ala Cys Gly Gly Ala Thr Cys Cys Ala Cys Cys
        755                 760                 765             
Thr Thr Cys Ala Ala Ala Thr Cys Ala Thr Ala Thr Gly Cys Thr Gly
    770                 775                 780                 
Ala Ala Gly Cys Thr Ala Ala Cys Ala Thr Cys Cys Cys Thr Thr Thr
785                 790                 795                 800 
Ala Gly Cys Cys Thr Ala Cys Thr Ala Thr Thr Thr Cys Thr Ala Thr
                805                 810                 815     
Gly Ala Gly Ala Ala Cys Gly Ala Ala Gly Ala Ala Cys Ala Ala Cys
            820                 825                 830         
Gly Thr Gly Cys Thr Gly Cys Thr Gly Cys Thr Gly Cys Cys Gly Ala
        835                 840                 845             
Thr Ala Thr Thr Ala Thr Thr Ala Ala Ala Cys Cys Thr Thr Thr Thr
    850                 855                 860                 
Gly Cys Thr Ala Ala Ala Gly Ala Gly Cys Ala Ala Cys Gly Thr Gly
865                 870                 875                 880 
Gly Cys Ala Ala Ala Ala Thr Thr Ala Ala Cys Thr Thr Thr Gly Thr
                885                 890                 895     
Thr Gly Gly Cys Thr Thr Ala Gly Ala Thr Gly Cys Cys Gly Thr Thr
            900                 905                 910         
Ala Ala Ala Thr Thr Cys Gly Gly Thr Ala Ala Gly Cys Ala Thr Gly
        915                 920                 925             
Cys Cys Ala Ala Gly Ala Ala Cys Thr Thr Ala Ala Ala Cys Ala Thr
    930                 935                 940                 
Gly Gly Ala Thr Gly Ala Ala Gly Ala Gly Ala Ala Ala Cys Thr Cys
945                 950                 955                 960 
Cys Cys Thr Cys Thr Ala Thr Thr Thr Gly Thr Cys Ala Thr Thr Cys
                965                 970                 975     
Ala Thr Gly Ala Thr Thr Thr Gly Gly Thr Gly Ala Gly Cys Ala Ala
            980                 985                 990         
Cys Ala Ala Gly Ala Ala Gly Thr Thr Thr Gly Gly Ala Gly Thr Thr
        995                 1000                1005            
Cys Cys Thr Cys Ala Ala Gly Ala Cys Cys Ala Ala Gly Ala Ala Thr
    1010                1015                1020                
Thr Gly Ala Cys Gly Ala Ala Cys Ala Ala Ala Gly Ala Thr Gly Thr
1025                1030                1035               1040 
Gly Ala Cys Cys Gly Ala Gly Cys Thr Gly Ala Thr Thr Gly Ala Gly
                1045                1050                1055    
Ala Ala Ala Thr Thr Cys Ala Thr Cys Gly Cys Ala Gly Gly Ala Gly
            1060                1065                1070        
Ala Gly Gly Cys Ala Gly Ala Ala Cys Cys Ala Ala Thr Thr Gly Thr
        1075                1080                1085            
Gly Ala Ala Ala Thr Cys Ala Gly Ala Gly Cys Cys Ala Ala Thr Thr
    1090                1095                1100                
Cys Cys Ala Gly Ala Ala Ala Thr Thr Cys Ala Ala Gly Ala Ala Gly
1105                1110                1115               1120 
Ala Gly Ala Ala Ala Gly Thr Cys Thr Thr Cys Ala Ala Gly Cys Thr
                1125                1130                1135    
Ala Gly Thr Cys Gly Gly Ala Ala Ala Gly Gly Cys Cys Cys Ala Cys
            1140                1145                1150        
Gly Ala Thr Gly Ala Ala Gly Thr Thr Gly Thr Cys Thr Thr Cys Gly
        1155                1160                1165            
Ala Thr Gly Ala Ala Thr Cys Thr Ala Ala Ala Gly Ala Thr Gly Thr
    1170                1175                1180                
Thr Cys Thr Ala Gly Thr Cys Ala Ala Gly Thr Ala Cys Thr Ala Cys
1185                1190                1195               1200 
Gly Cys Cys Cys Cys Thr Thr Gly Gly Thr Gly Thr Gly Gly Thr Cys
                1205                1210                1215    
Ala Cys Thr Gly Thr Ala Ala Gly Ala Gly Ala Ala Thr Gly Gly Cys
            1220                1225                1230        
Thr Cys Cys Thr Gly Cys Thr Thr Ala Thr Gly Ala Gly Gly Ala Ala
        1235                1240                1245            
Thr Thr Gly Gly Cys Thr Ala Cys Thr Cys Thr Thr Thr Ala Cys Gly
    1250                1255                1260                
Cys Cys Ala Ala Thr Gly Ala Thr Gly Ala Gly Gly Ala Thr Gly Cys
1265                1270                1275               1280 
Cys Thr Cys Thr Thr Cys Ala Ala Ala Gly Gly Thr Thr Gly Thr Gly
                1285                1290                1295    
Ala Thr Thr Gly Cys Ala Ala Ala Ala Cys Thr Thr Gly Ala Thr Cys
            1300                1305                1310        
Ala Cys Ala Cys Thr Thr Thr Gly Ala Ala Cys Gly Ala Thr Gly Thr
        1315                1320                1325            
Cys Gly Ala Cys Ala Ala Cys Gly Thr Thr Gly Ala Thr Ala Thr Thr
    1330                1335                1340                
Cys Ala Ala Gly Gly Thr Thr Ala Thr Cys Cys Thr Ala Cys Thr Thr
1345                1350                1355               1360 
Thr Gly Ala Thr Cys Cys Thr Thr Thr Ala Thr Cys Cys Ala Gly Cys
                1365                1370                1375    
Thr Gly Gly Thr Gly Ala Thr Ala Ala Ala Thr Cys Cys Ala Ala Thr
            1380                1385                1390        
Cys Cys Thr Cys Ala Ala Cys Thr Gly Thr Ala Thr Gly Ala Thr Gly
        1395                1400                1405            
Gly Ala Thr Cys Thr Cys Gly Thr Gly Ala Cys Cys Thr Ala Gly Ala
    1410                1415                1420                
Ala Thr Cys Ala Thr Thr Gly Gly Cys Thr Gly Ala Gly Thr Thr Thr
1425                1430                1435               1440 
Gly Thr Ala Ala Ala Gly Gly Ala Gly Ala Gly Ala Gly Gly Ala Ala
                1445                1450                1455    
Cys Cys Cys Ala Cys Ala Ala Ala Gly Thr Gly Gly Ala Thr Gly Cys
            1460                1465                1470        
Cys Cys Thr Ala Gly Cys Ala Cys Thr Cys Ala Gly Ala Cys Cys Ala
        1475                1480                1485            
Gly Thr Cys Gly Ala Gly Gly Ala Ala Gly Ala Ala Ala Ala Gly Gly
    1490                1495                1500                
Ala Ala Gly Cys Thr Gly Ala Ala Gly Ala Ala Gly Ala Ala Gly Cys
1505                1510                1515               1520 
Thr Gly Ala Ala Ala Gly Thr Gly Ala Gly Gly Cys Ala Gly Ala Cys
                1525                1530                1535    
Gly Cys Thr Cys Ala Cys Gly Ala Cys Gly Ala Gly Cys Thr Thr Thr
            1540                1545                1550        
Ala Ala
        


<210> 43
<211> 1337
<212> DNA
<213> Artificial Sequence

<220> 
<223> human ERO1alpha without leader

<400> 43
gaagaacaac caccagagac tgctgctcag agatgcttct gtcaggtttc cggttacttg 60
gacgactgta cttgtgacgt tgagactatc gacagattca acaactacag attgttccca 120
agattgcaga agttgttgga gtccgactac ttcagatact acaaggttaa cttgaagaga 180
ccatgtccat tctggaacga catttcccag tgtggtagaa gagactgtgc tgttaagcca 240
tgtcaatccg acgaagttcc agacggtatt aagtccgctt cctacaagta ctctgaagag 300
gctaacaact tgatcgaaga gtgtgagcaa gctgaaagat tgggtgctgt tgacgaatct 360
ttgtccgaga gactcagaag gctgttttgc agtggactaa gcacgatgat tcctccgaca 420
acttctgtga agctgacgac attcaatctc cagaggctga gtacgttgac ttgttgttga 480
acccagagag atacactggt tacaagggtc cagacgcttg gaagatttgg aacgttatct 540
acgaagagaa ctgtttcaag ccacagacta tcaagagacc attgaaccca ttggcttccg 600
gacagggaac ttctgaagag aacactttct actcttggtt ggagggtttg tgtgttgaga 660
agagagcttt ctacagattg atctccggat tgcacgcttc tatcaacgtt cacttgtccg 720
ctagatactt gttgcaagag acttggttgg aaaagaagtg gggtcacaac attactgagt 780
tccagcagag attcgacggt attttgactg aaggtgaagg tccaagaaga ttgaagaact 840
tgtacttttt gtacttgatc gagttgagag ctttgtccaa ggttttgcca ttcttcgaga 900
gaccagactt ccaattgttc actggtaaca agatccagga cgaagagaac aagatgttgt 960
tgttggagat tttgcacgag atcaagtcct ttccattgca cttcgacgag aactcatttt 1020
tcgctggtga caagaaagaa gctcacaagt tgaaagagga cttcagattg cacttcagaa 1080
atatctccag aatcatggac tgtgttggtt gtttcaagtg tagattgtgg ggtaagttgc 1140
agactcaagg attgggtact gctttgaaga ttttgttctc cgagaagttg atcgctaaca 1200
tgcctgaatc tggtccatct tacgagttcc acttgactag acaagagatc gtttccttgt 1260
tcaacgcttt cggtagaatc tccacttccg ttaaagagtt ggagaacttc agaaacttgt 1320
tgcagaacat ccactaa                                                1337

<210> 44
<211> 445
<212> PRT
<213> Artificial Sequence

<220> 
<223> human ERO1alpha without leader

<400> 44
Glu Glu Gln Pro Pro Glu Thr Ala Ala Gln Arg Cys Phe Cys Gln Val
 1               5                  10                  15      
Ser Gly Tyr Leu Asp Asp Cys Thr Cys Asp Val Glu Thr Ile Asp Arg
            20                  25                  30          
Phe Asn Asn Tyr Arg Leu Phe Pro Arg Leu Gln Lys Leu Leu Glu Ser
        35                  40                  45              
Asp Tyr Phe Arg Tyr Tyr Lys Val Asn Leu Lys Arg Pro Cys Pro Phe
    50                  55                  60                  
Trp Asn Asp Ile Ser Gln Cys Gly Arg Arg Asp Cys Ala Val Lys Pro
65                  70                  75                  80  
Cys Gln Ser Asp Glu Val Pro Asp Gly Ile Lys Ser Ala Ser Tyr Lys
                85                  90                  95      
Tyr Ser Glu Glu Ala Asn Asn Leu Ile Glu Glu Cys Glu Gln Ala Glu
            100                 105                 110         
Arg Leu Gly Ala Val Asp Glu Ser Leu Ser Glu Glu Thr Gln Lys Ala
        115                 120                 125             
Val Leu Gln Trp Thr Lys His Asp Asp Ser Ser Asp Asn Phe Cys Glu
    130                 135                 140                 
Ala Asp Asp Ile Gln Ser Pro Glu Ala Glu Tyr Val Asp Leu Leu Leu
145                 150                 155                 160 
Asn Pro Glu Arg Tyr Thr Gly Tyr Lys Gly Pro Asp Ala Trp Lys Ile
                165                 170                 175     
Trp Asn Val Ile Tyr Glu Glu Asn Cys Phe Lys Pro Gln Thr Ile Lys
            180                 185                 190         
Arg Pro Leu Asn Pro Leu Ala Ser Gly Gln Gly Thr Ser Glu Glu Asn
        195                 200                 205             
Thr Phe Tyr Ser Trp Leu Glu Gly Leu Cys Val Glu Lys Arg Ala Phe
    210                 215                 220                 
Tyr Arg Leu Ile Ser Gly Leu His Ala Ser Ile Asn Val His Leu Ser
225                 230                 235                 240 
Ala Arg Tyr Leu Leu Gln Glu Thr Trp Leu Glu Lys Lys Trp Gly His
                245                 250                 255     
Asn Ile Thr Glu Phe Gln Gln Arg Phe Asp Gly Ile Leu Thr Glu Gly
            260                 265                 270         
Glu Gly Pro Arg Arg Leu Lys Asn Leu Tyr Phe Leu Tyr Leu Ile Glu
        275                 280                 285             
Leu Arg Ala Leu Ser Lys Val Leu Pro Phe Phe Glu Arg Pro Asp Phe
    290                 295                 300                 
Gln Leu Phe Thr Gly Asn Lys Ile Gln Asp Glu Glu Asn Lys Met Leu
305                 310                 315                 320 
Leu Leu Glu Ile Leu His Glu Ile Lys Ser Phe Pro Leu His Phe Asp
                325                 330                 335     
Glu Asn Ser Phe Phe Ala Gly Asp Lys Lys Glu Ala His Lys Leu Lys
            340                 345                 350         
Glu Asp Phe Arg Leu His Phe Arg Asn Ile Ser Arg Ile Met Asp Cys
        355                 360                 365             
Val Gly Cys Phe Lys Cys Arg Leu Trp Gly Lys Leu Gln Thr Gln Gly
    370                 375                 380                 
Leu Gly Thr Ala Leu Lys Ile Leu Phe Ser Glu Lys Leu Ile Ala Asn
385                 390                 395                 400 
Met Pro Glu Ser Gly Pro Ser Tyr Glu Phe His Leu Thr Arg Gln Glu
                405                 410                 415     
Ile Val Ser Leu Phe Asn Ala Phe Gly Arg Ile Ser Thr Ser Val Lys
            420                 425                 430         
Glu Leu Glu Asn Phe Arg Asn Leu Leu Gln Asn Ile His
        435                 440                 445 


<210> 45
<211> 2349
<212> DNA
<213> Artificial Sequence

<220> 
<223> human GRP94 without leader

<400> 45
gatgatgaag ttgacgttga cggtactgtt gaagaggact tgggaaagtc tagagagggt 60
tccagaactg acgacgaagt tgttcagaga gaggaagagg ctattcagtt ggacggattg 120
aacgcttccc aaatcagaga gttgagagag aagtccgaga agttcgcttt ccaagctgag 180
gttaacagaa tgatgaaatt gattatcaac tccttgtaca agaacaaaga gattttcttg 240
agagagttga tctctaacgc ttctgacgct ttggacaaga tcagattgat ctccttgact 300
gacgaaaacg ctttgtccgg taacgaagag ttgactgtta agatcaagtg tgacaaagag 360
aagaacttgt tgcacgttac tgacactggt gttggaatga ctagagaaga gttggttaag 420
aacttgggta ctatcgctaa gtctggtact tccgagttct tgaacaagat gactgaggct 480
caagaagatg gtcaatccac ttccgagttg attggtcagt tcggtgttgg tttctactcc 540
gctttcttgg ttgctgacaa ggttatcgtt acttccaagc acaacaacga cactcaacac 600
atttgggaat ccgattccaa cgagttctcc gttattgctg acccaagagg taacactttg 660
ggtagaggta ctactatcac tttggttttg aaagaagagg cttccgacta cttggagttg 720
gacactatca agaacttggt taagaagtac tcccagttca tcaacttccc aatctatgtt 780
tggtcctcca agactgagac tgttgaggaa ccaatggaag aagaagaggc tgctaaagaa 840
gagaaagagg aatctgacga cgaggctgct gttgaagaag aggaagaaga aaagaagcca 900
aagactaaga aggttgaaaa gactgtttgg gactgggagc ttatgaacga catcaagcca 960
atttggcaga gaccatccaa agaggttgag gaggacgagt acaaggcttt ctacaagtcc 1020
ttctccaaag aatccgatga cccaatggct tacatccact tcactgctga gggtgaagtt 1080
actttcaagt ccatcttgtt cgttccaact tctgctccaa gaggattgtt cgacgagtac 1140
ggttctaaga agtccgacta catcaaactt tatgttagaa gagttttcat cactgacgac 1200
ttccacgata tgatgccaaa gtacttgaac ttcgttaagg gtgttgttga ttccgatgac 1260
ttgccattga acgtttccag agagactttg cagcagcaca agttgttgaa ggttatcaga 1320
aagaaacttg ttagaaagac tttggacatg atcaagaaga tcgctgacga caagtacaac 1380
gacactttct ggaaagagtt cggaactaac atcaagttgg gtgttattga ggaccactcc 1440
aacagaacta gattggctaa gttgttgaga ttccagtcct ctcatcaccc aactgacatc 1500
acttccttgg accagtacgt tgagagaatg aaagagaagc aggacaaaat ctacttcatg 1560
gctggttcct ctagaaaaga ggctgaatcc tccccattcg ttgagagatt gttgaagaag 1620
ggttacgagg ttatctactt gactgagcca gttgacgagt actgtatcca ggctttgcca 1680
gagtttgacg gaaagagatt ccagaacgtt gctaaagagg gtgttaagtt cgacgaatcc 1740
gaaaagacta aagaatccag agaggctgtt gagaaagagt tcgagccatt gttgaactgg 1800
atgaaggaca aggctttgaa ggacaagatc gagaaggctg ttgtttccca gagattgact 1860
gaatccccat gtgctttggt tgcttcccaa tacggatgga gtggtaacat ggaaagaatc 1920
atgaaggctc aggcttacca aactggaaag gacatctcca ctaactacta cgcttcccag 1980
aagaaaactt tcgagatcaa cccaagacac ccattgatca gagacatgtt gagaagaatc 2040
aaagaggacg aggacgacaa gactgttttg gatttggctg ttgttttgtt cgagactgct 2100
actttgagat ccggttactt gttgccagac actaaggctt acggtgacag aatcgagaga 2160
atgttgagat tgtccttgaa cattgaccca gacgctaagg ttgaagaaga accagaagaa 2220
gagccagagg aaactgctga agatactact gaggacactg aacaagacga ggacgaagag 2280
atggatgttg gtactgacga agaggaagag acagcaaagg aatccactgc tgaacacgac 2340
gagttgtaa                                                         2349

<210> 46
<211> 782
<212> PRT
<213> Artificial Sequence

<220> 
<223> human GRP94 without leader

<400> 46
Asp Asp Glu Val Asp Val Asp Gly Thr Val Glu Glu Asp Leu Gly Lys
 1               5                  10                  15      
Ser Arg Glu Gly Ser Arg Thr Asp Asp Glu Val Val Gln Arg Glu Glu
            20                  25                  30          
Glu Ala Ile Gln Leu Asp Gly Leu Asn Ala Ser Gln Ile Arg Glu Leu
        35                  40                  45              
Arg Glu Lys Ser Glu Lys Phe Ala Phe Gln Ala Glu Val Asn Arg Met
    50                  55                  60                  
Met Lys Leu Ile Ile Asn Ser Leu Tyr Lys Asn Lys Glu Ile Phe Leu
65                  70                  75                  80  
Arg Glu Leu Ile Ser Asn Ala Ser Asp Ala Leu Asp Lys Ile Arg Leu
                85                  90                  95      
Ile Ser Leu Thr Asp Glu Asn Ala Leu Ser Gly Asn Glu Glu Leu Thr
            100                 105                 110         
Val Lys Ile Lys Cys Asp Lys Glu Lys Asn Leu Leu His Val Thr Asp
        115                 120                 125             
Thr Gly Val Gly Met Thr Arg Glu Glu Leu Val Lys Asn Leu Gly Thr
    130                 135                 140                 
Ile Ala Lys Ser Gly Thr Ser Glu Phe Leu Asn Lys Met Thr Glu Ala
145                 150                 155                 160 
Gln Glu Asp Gly Gln Ser Thr Ser Glu Leu Ile Gly Gln Phe Gly Val
                165                 170                 175     
Gly Phe Tyr Ser Ala Phe Leu Val Ala Asp Lys Val Ile Val Thr Ser
            180                 185                 190         
Lys His Asn Asn Asp Thr Gln His Ile Trp Glu Ser Asp Ser Asn Glu
        195                 200                 205             
Phe Ser Val Ile Ala Asp Pro Arg Gly Asn Thr Leu Gly Arg Gly Thr
    210                 215                 220                 
Thr Ile Thr Leu Val Leu Lys Glu Glu Ala Ser Asp Tyr Leu Glu Leu
225                 230                 235                 240 
Asp Thr Ile Lys Asn Leu Val Lys Lys Tyr Ser Gln Phe Ile Asn Phe
                245                 250                 255     
Pro Ile Tyr Val Trp Ser Ser Lys Thr Glu Thr Val Glu Glu Pro Met
            260                 265                 270         
Glu Glu Glu Glu Ala Ala Lys Glu Glu Lys Glu Glu Ser Asp Asp Glu
        275                 280                 285             
Ala Ala Val Glu Glu Glu Glu Glu Glu Lys Lys Pro Lys Thr Lys Lys
    290                 295                 300                 
Val Glu Lys Thr Val Trp Asp Trp Glu Leu Met Asn Asp Ile Lys Pro
305                 310                 315                 320 
Ile Trp Gln Arg Pro Ser Lys Glu Val Glu Glu Asp Glu Tyr Lys Ala
                325                 330                 335     
Phe Tyr Lys Ser Phe Ser Lys Glu Ser Asp Asp Pro Met Ala Tyr Ile
            340                 345                 350         
His Phe Thr Ala Glu Gly Glu Val Thr Phe Lys Ser Ile Leu Phe Val
        355                 360                 365             
Pro Thr Ser Ala Pro Arg Gly Leu Phe Asp Glu Tyr Gly Ser Lys Lys
    370                 375                 380                 
Ser Asp Tyr Ile Lys Leu Tyr Val Arg Arg Val Phe Ile Thr Asp Asp
385                 390                 395                 400 
Phe His Asp Met Met Pro Lys Tyr Leu Asn Phe Val Lys Gly Val Val
                405                 410                 415     
Asp Ser Asp Asp Leu Pro Leu Asn Val Ser Arg Glu Thr Leu Gln Gln
            420                 425                 430         
His Lys Leu Leu Lys Val Ile Arg Lys Lys Leu Val Arg Lys Thr Leu
        435                 440                 445             
Asp Met Ile Lys Lys Ile Ala Asp Asp Lys Tyr Asn Asp Thr Phe Trp
    450                 455                 460                 
Lys Glu Phe Gly Thr Asn Ile Lys Leu Gly Val Ile Glu Asp His Ser
465                 470                 475                 480 
Asn Arg Thr Arg Leu Ala Lys Leu Leu Arg Phe Gln Ser Ser His His
                485                 490                 495     
Pro Thr Asp Ile Thr Ser Leu Asp Gln Tyr Val Glu Arg Met Lys Glu
            500                 505                 510         
Lys Gln Asp Lys Ile Tyr Phe Met Ala Gly Ser Ser Arg Lys Glu Ala
        515                 520                 525             
Glu Ser Ser Pro Phe Val Glu Arg Leu Leu Lys Lys Gly Tyr Glu Val
    530                 535                 540                 
Ile Tyr Leu Thr Glu Pro Val Asp Glu Tyr Cys Ile Gln Ala Leu Pro
545                 550                 555                 560 
Glu Phe Asp Gly Lys Arg Phe Gln Asn Val Ala Lys Glu Gly Val Lys
                565                 570                 575     
Phe Asp Glu Ser Glu Lys Thr Lys Glu Ser Arg Glu Ala Val Glu Lys
            580                 585                 590         
Glu Phe Glu Pro Leu Leu Asn Trp Met Lys Asp Lys Ala Leu Lys Asp
        595                 600                 605             
Lys Ile Glu Lys Ala Val Val Ser Gln Arg Leu Thr Glu Ser Pro Cys
    610                 615                 620                 
Ala Leu Val Ala Ser Gln Tyr Gly Trp Ser Gly Asn Met Glu Arg Ile
625                 630                 635                 640 
Met Lys Ala Gln Ala Tyr Gln Thr Gly Lys Asp Ile Ser Thr Asn Tyr
                645                 650                 655     
Tyr Ala Ser Gln Lys Lys Thr Phe Glu Ile Asn Pro Arg His Pro Leu
            660                 665                 670         
Ile Arg Asp Met Leu Arg Arg Ile Lys Glu Asp Glu Asp Asp Lys Thr
        675                 680                 685             
Val Leu Asp Leu Ala Val Val Leu Phe Glu Thr Ala Thr Leu Arg Ser
    690                 695                 700                 
Gly Tyr Leu Leu Pro Asp Thr Lys Ala Tyr Gly Asp Arg Ile Glu Arg
705                 710                 715                 720 
Met Leu Arg Leu Ser Leu Asn Ile Asp Pro Asp Ala Lys Val Glu Glu
                725                 730                 735     
Glu Pro Glu Glu Glu Pro Glu Glu Thr Ala Glu Asp Thr Thr Glu Asp
            740                 745                 750         
Thr Glu Gln Asp Glu Asp Glu Glu Met Asp Val Gly Thr Asp Glu Glu
        755                 760                 765             
Glu Glu Thr Ala Lys Glu Ser Thr Ala Glu His Asp Glu Leu
    770                 775                 780         


<210> 47
<211> 8448
<212> DNA
<213> Pichia pastoris

<220> 
<221> CDS            
<222> (3016)...(5385)
<223> Encodes PMT1

<400> 47
actttttcaa ttcctcaggg tactccgttg gaattctgta cttagcagca tactgatctt 60
tgaccaccca aggagcacca gatctttgcg atctagtcaa cgtcaacttg agaaaagttt 120
tcacgtacca cttagtgaac gcattcctat cacgggaaac ttgattttcg ttcacggtta 180
cttctccatc agagtttgag aggccaacgc gataagagca gtatccttca cgtacggtac 240
catcaggtaa ggtgatggga gcaaaccgtg ccttttctct gatgatccct ttatatctgt 300
tagatccagc acttttaaca ttcactagat ccccaggaaa aaattctttc ttgaagtgta 360
aatacacgtc atcgactaat tgatctagtc tgggtatgag actgaattgc acatatctca 420
aaattggttc tctgacaggt tctggaaact tattttcaac cgcttgcatt tcctcctgtt 480
cgtacttgag ggcctcaaag aaatcaaacg agctgtttcc agtgatttca cacgcaaact 540
tcttttggct ataatagtcc atcctatcaa ggtactcatc gtagttcaaa aaccactcgc 600
cagtctgtgg aatgtaccaa atttccgtgt ctagatcatc aggaagctgt tgtggaggga 660
caacttccac ctgctttctt ttgaagagaa ccatggtgtt tggggattag aagaaaacaa 720
atatttgagc ggaacttgcg aaaaaacgcc cctagcgaat gcaagctaga catgtcagga 780
agataaaatt gataccgcag aagcaggggt agttggggag ggcaatcaag tacgttcaca 840
gagcatggct gcgttatcaa ctgactattt tatggcgtgg tttagaagag agagtatcaa 900
ttaggcgtca actgggacca ttatgattag acgttgtagg tagatgcagg tgaaaaatgg 960
acagacgtag gcaacaaaca caaactgtcg ggtaaccttt aacagtattc aattccaggt 1020
gtttcaagac agccttagat actagcaagc ttccagggaa accctattac tcatgctccc 1080
actgttggaa ctcacaacca agaggctaca tgtatgcgta tgcatacagg tactgctcag 1140
tgataaattt atttcgcgag atcgtactcc agaaactttc atgtaagcct tcctacttcg 1200
ctctgcccac tatgttagcc agaaaggtat tagctagaca atgtctggtg gtagccaggc 1260
tttgtgcggg tagatttgcc tcctcattat gcgggtgcag ttgtagaggt ttgatgaggc 1320
caccaaaatt taacagttcc aaatctcttt cgagatcgat gacctcatcg tccctgtttg 1380
agtctccaaa ttgtccttcc tgtggtgtgg ttctccaaac agaacatcca gacaaagatg 1440
ggtattgtct actgcccaaa ggtgaaagga aagttaaaaa ttatcaaaat gaactaaaag 1500
aaaagctttt tttgaatgtg aaaagggaag aacttgccga cagactgggc catgaggtgg 1560
actctgaatc actgattata cccaaggaaa tgtaccaaaa gccccgtacc ccgaaacgac 1620
tggtttgtca gagatgcttc aaatcgcaaa actattcctt gatcgaccat tccattcgtg 1680
aagaaaatcc cgaacacaag atcctggatg agatcccttc aaacgccaat atcgtccacg 1740
ttttatctgc tgttgatttt cctcttggtc tcagcaagga actggtaaac agatttaaac 1800
ccactcagat tacgtacgtt attacaaagt ctgacgtgtt cttccccgat aagctaggtc 1860
tccaacggac gggagctgct tattttgaag acagcttggt aaagcttgtc ggtgcagatc 1920
ctagaaaggt agtattggtc tcaggaaaaa gaaattgggg cctcaaacag ctgctatcca 1980
ctttacccag aggtcccaat tactttctgg gaatgacgaa caccggaaaa tcaaccctaa 2040
tacgatccat cgttggtaag gattactcaa agaagcagac agagaatggc ccgggtgtct 2100
ctcaccttcc ttcattcaca agaaaaccca tgaagttcaa aatggacaac aacagtcttg 2160
aactcgtaga tctccctgga tacactgctc caaatggagg tgtttacaag tatcttaagg 2220
aagagaacta ccgagacatt ttgaacgtta aacagttaaa gccattgaca tccctcaagg 2280
catacacaga aacgttgcct tcgaagccaa aactattcaa tggtgtgcga gtaatatgca 2340
ttggtggttt agtgtacatt cggcccccaa agggtgtagt gctgaaacag tttagtctcg 2400
tcaaccttcc atccttcatg tactcgtcgc taaaaaaggc caccagtgta atccaagcgc 2460
ccccacaagc cttggtgaat tgcagcgtcg tcaaggagga cagtccagat gaactggtaa 2520
gatatgtgat ccctccattt tatggtttaa ttgacctggt cattcaaggt gttggattta 2580
tcaagcttct gcccactgga gctcggaaca ccagagaact gatagaaatt tttgccccaa 2640
aagacatcca gctcatggtg cgtgattcca tcctcaaata cgtctacaag acccatgccg 2700
aacacgactc aaccaataat ctcctgcata aaaagaacat aaaagccaga ggccaaacca 2760
tactacgaag actacccaaa aagcctgtat tcacaaagct ttttcccgta ccagccaacg 2820
taccgtctca tgaactgctc accatggtga cgggaaagga cgacctagcc gaggaagaca 2880
aagaataccg ctacgatatc cagtatccca acagatactg ggatgaaacc atctgtaaat 2940
agaatgctta tgtaatcaag cactttctga aattccttag agtttcgcgt gtctccccgt 3000
caaaaatcgc gtctc atg tgc cag ata ttt ctc ccg caa aac gta aca cgt  3051
                 Met Cys Gln Ile Phe Leu Pro Gln Asn Val Thr Arg
                  1               5                   10
 
tgt tct gtt tcc ctt ttg aca atg agt aaa aca agt cct caa gag gtg   3099
Cys Ser Val Ser Leu Leu Thr Met Ser Lys Thr Ser Pro Gln Glu Val
         15                  20                  25
 
cca gaa aac act act gag ctt aaa atc tca aaa gga gag ctc cgt cct   3147
Pro Glu Asn Thr Thr Glu Leu Lys Ile Ser Lys Gly Glu Leu Arg Pro
     30                  35                  40
 
ttt att gtg acc tct cca tct cct caa ttg agc aag tct cgt tct gtg   3195
Phe Ile Val Thr Ser Pro Ser Pro Gln Leu Ser Lys Ser Arg Ser Val
 45                  50                  55                  60
 
act tca acc aag gag aag ctg ata ttg gct agt ttg ttc ata ttt gca   3243
Thr Ser Thr Lys Glu Lys Leu Ile Leu Ala Ser Leu Phe Ile Phe Ala
                 65                  70                  75
 
atg gtc atc agg ttc cac aac gtc gcc cac cct gac agc gtt gtg ttt   3291
Met Val Ile Arg Phe His Asn Val Ala His Pro Asp Ser Val Val Phe
             80                  85                  90
 
gat gaa gtt cac ttt ggg ggg ttt gcc aga aag tac att ttg gga acc   3339
Asp Glu Val His Phe Gly Gly Phe Ala Arg Lys Tyr Ile Leu Gly Thr
         95                 100                 105
 
ttt ttc atg gat gtt cat ccg cca ttg gcc aag cta tta ttt gct ggt   3387
Phe Phe Met Asp Val His Pro Pro Leu Ala Lys Leu Leu Phe Ala Gly
    110                 115                 120
 
gtt ggc agt ctt ggt gga tac gat gga gag ttt gag ttc aag aaa att   3435
Val Gly Ser Leu Gly Gly Tyr Asp Gly Glu Phe Glu Phe Lys Lys Ile
125                 130                 135                 140
 
ggt gac gaa ttc cca gag aat gtt cct tat gtg ctc atg aga tat ctt   3483
Gly Asp Glu Phe Pro Glu Asn Val Pro Tyr Val Leu Met Arg Tyr Leu
                145                 150                 155
 
ccc tct ggt atg gga gtt gga aca tgt att atg ttg tat ttg act ctg   3531
Pro Ser Gly Met Gly Val Gly Thr Cys Ile Met Leu Tyr Leu Thr Leu
            160                 165                 170
 
aga gct tct ggt tgt caa cca ata gtc tgt gct ctg aca acc gct ctt   3579
Arg Ala Ser Gly Cys Gln Pro Ile Val Cys Ala Leu Thr Thr Ala Leu
        175                 180                 185
 
ttg atc att gag aat gct aat gtt aca atc tcc aga ttc att ttg ctg   3627
Leu Ile Ile Glu Asn Ala Asn Val Thr Ile Ser Arg Phe Ile Leu Leu
    190                 195                 200
 
gat tcg cca atg ctg ttt ttt att gct tca aca gtt tac tct ttc aag   3675
Asp Ser Pro Met Leu Phe Phe Ile Ala Ser Thr Val Tyr Ser Phe Lys
205                 210                 215                 220
 
aaa ttt caa att cag gaa ccg ttt acc ttc caa tgg tac aag acc ctt   3723
Lys Phe Gln Ile Gln Glu Pro Phe Thr Phe Gln Trp Tyr Lys Thr Leu
                225                 230                 235
 
att gct act ggt gtt tct tta ggg tta gca gct tcc agt aaa tgg gtt   3771
Ile Ala Thr Gly Val Ser Leu Gly Leu Ala Ala Ser Ser Lys Trp Val
            240                 245                 250
 
ggt ttg ttc acc gtt gcc tgg att gga ttg ata aca att tgg gac tta   3819
Gly Leu Phe Thr Val Ala Trp Ile Gly Leu Ile Thr Ile Trp Asp Leu
        255                 260                 265
 
tgg ttc atc att ggt gat ttg act gtt tct gta aag aaa att ttc ggc   3867
Trp Phe Ile Ile Gly Asp Leu Thr Val Ser Val Lys Lys Ile Phe Gly
    270                 275                 280
 
cat ttt atc acc aga gct gta gct ttc tta gtc gtc ccc act ctg atc   3915
His Phe Ile Thr Arg Ala Val Ala Phe Leu Val Val Pro Thr Leu Ile
285                 290                 295                 300
 
tac ctc act ttc ttt gcc atc cat ttg caa gtc tta acc aag gaa ggt   3963
Tyr Leu Thr Phe Phe Ala Ile His Leu Gln Val Leu Thr Lys Glu Gly
                305                 310                 315
 
gat ggt ggt gct ttc atg tct tcc gtc ttc aga tcg acc tta gaa ggt   4011
Asp Gly Gly Ala Phe Met Ser Ser Val Phe Arg Ser Thr Leu Glu Gly
            320                 325                 330
 
aat gct gtt cca aaa cag tcg ctg gcc aac gtt ggt ttg ggc tct tta   4059
Asn Ala Val Pro Lys Gln Ser Leu Ala Asn Val Gly Leu Gly Ser Leu
        335                 340                 345
 
gtc act atc cgt cat ttg aac acc aga ggt ggt tac tta cac tct cac   4107
Val Thr Ile Arg His Leu Asn Thr Arg Gly Gly Tyr Leu His Ser His
    350                 355                 360
 
aat cat ctt tac gag ggt ggt tct ggt caa cag cag gtc acc ttg tac   4155
Asn His Leu Tyr Glu Gly Gly Ser Gly Gln Gln Gln Val Thr Leu Tyr
365                 370                 375                 380
 
cca cac att gat tct aat aat caa tgg att gta cag gat tac aac gcg   4203
Pro His Ile Asp Ser Asn Asn Gln Trp Ile Val Gln Asp Tyr Asn Ala
                385                 390                 395
 
act gag gag cca act gaa ttt gtt cca ttg aaa gac ggt gtc aaa atc   4251
Thr Glu Glu Pro Thr Glu Phe Val Pro Leu Lys Asp Gly Val Lys Ile
            400                 405                 410
 
aga tta aac cac aaa ttg act tcc cga aga ttg cac tct cat aac ctc   4299
Arg Leu Asn His Lys Leu Thr Ser Arg Arg Leu His Ser His Asn Leu
        415                 420                 425
 
aga cct cct gtg act gaa caa gat tgg caa aat gag gta tct gct tat   4347
Arg Pro Pro Val Thr Glu Gln Asp Trp Gln Asn Glu Val Ser Ala Tyr
    430                 435                 440
 
gga cat gag ggc ttt ggc ggt gat gcc aat gat gac ttt gtt gtg gag   4395
Gly His Glu Gly Phe Gly Gly Asp Ala Asn Asp Asp Phe Val Val Glu
445                 450                 455                 460
 
att gcc aag gat ctt tca act act gaa gaa gct aag gaa aac gtt agg   4443
Ile Ala Lys Asp Leu Ser Thr Thr Glu Glu Ala Lys Glu Asn Val Arg
                465                 470                 475
 
gcc att caa act gtt ttt aga ttg aga cat gcg atg act ggt tgt tac   4491
Ala Ile Gln Thr Val Phe Arg Leu Arg His Ala Met Thr Gly Cys Tyr
            480                 485                 490
 
ttg ttc tcc cac gaa gtc aag ctt ccc aag tgg gca tat gag caa caa   4539
Leu Phe Ser His Glu Val Lys Leu Pro Lys Trp Ala Tyr Glu Gln Gln
        495                 500                 505
 
gag gtt act tgt gct act caa ggt atc aaa cca cta tct tac tgg tac   4587
Glu Val Thr Cys Ala Thr Gln Gly Ile Lys Pro Leu Ser Tyr Trp Tyr
    510                 515                 520
 
gtt gag acc aac gaa aac cca ttc ttg gat aaa gag gtt gat gaa ata   4635
Val Glu Thr Asn Glu Asn Pro Phe Leu Asp Lys Glu Val Asp Glu Ile
525                 530                 535                 540
 
gtt agc tat cct gtt ccg act ttc ttt caa aag gtt gcc gag cta cac   4683
Val Ser Tyr Pro Val Pro Thr Phe Phe Gln Lys Val Ala Glu Leu His
                545                 550                 555
 
gcc aga atg tgg aag atc aac aag ggc tta act gat cat cat gtc tat   4731
Ala Arg Met Trp Lys Ile Asn Lys Gly Leu Thr Asp His His Val Tyr
            560                 565                 570
 
gaa tcc agt cca gat tct tgg ccc ttc ctg ctc aga ggt ata agc tac   4779
Glu Ser Ser Pro Asp Ser Trp Pro Phe Leu Leu Arg Gly Ile Ser Tyr
        575                 580                 585
 
tgg tca aaa aat cac tca caa att tat ttc ata ggt aat gct gtc act   4827
Trp Ser Lys Asn His Ser Gln Ile Tyr Phe Ile Gly Asn Ala Val Thr
    590                 595                 600
 
tgg tgg aca gtc acc gca agt att gct ttg ttc tct gtc ttt ttg gtt   4875
Trp Trp Thr Val Thr Ala Ser Ile Ala Leu Phe Ser Val Phe Leu Val
605                 610                 615                 620
 
ttc tct att ctg aga tgg caa aga ggt ttt ggg ttc agc gtt gac cca   4923
Phe Ser Ile Leu Arg Trp Gln Arg Gly Phe Gly Phe Ser Val Asp Pro
                625                 630                 635
 
act gtg ttc aac ttc aat gtt caa atg ctt cat tac atc cta gga tgg   4971
Thr Val Phe Asn Phe Asn Val Gln Met Leu His Tyr Ile Leu Gly Trp
            640                 645                 650
 
gta ctg cat tac ttg cca tct ttc ctt atg gcc cgt cag cta ttt ttg   5019
Val Leu His Tyr Leu Pro Ser Phe Leu Met Ala Arg Gln Leu Phe Leu
        655                 660                 665
 
cac cac tat cta cca tca ttg tac ttt ggt ata ttg gct ctc gga cat   5067
His His Tyr Leu Pro Ser Leu Tyr Phe Gly Ile Leu Ala Leu Gly His
    670                 675                 680
 
gtg ttt gag att att cac tct tat gtc ttc aaa aac aaa cag gtt gtg   5115
Val Phe Glu Ile Ile His Ser Tyr Val Phe Lys Asn Lys Gln Val Val
685                 690                 695                 700
 
tct tac tcc ata ttc gtt ctc ttt ttt gcc gtt gcg ctt tct ttc ttc   5163
Ser Tyr Ser Ile Phe Val Leu Phe Phe Ala Val Ala Leu Ser Phe Phe
                705                 710                 715
 
caa aga tat tct cca ttg atc tat gca gga cga tgg acc aag gac caa   5211
Gln Arg Tyr Ser Pro Leu Ile Tyr Ala Gly Arg Trp Thr Lys Asp Gln
            720                 725                 730
 
tgc aac gaa tcc aag ata ctc aag tgg gac ttt gac tgt aac acc ttc   5259
Cys Asn Glu Ser Lys Ile Leu Lys Trp Asp Phe Asp Cys Asn Thr Phe
        735                 740                 745
 
ccc agt cac aca tct cag tat gaa ata tgg gca tcc cct gta caa act   5307
Pro Ser His Thr Ser Gln Tyr Glu Ile Trp Ala Ser Pro Val Gln Thr
    750                 755                 760
 
tcc act cct aaa gaa gga acc cac tca gaa tct acc gtc gga gaa cct   5355
Ser Thr Pro Lys Glu Gly Thr His Ser Glu Ser Thr Val Gly Glu Pro
765                 770                 775                 780
 
gac gtt gag aag ctg gga gag aca gtc taa gctgtgttta tatagccctg     5405
Asp Val Glu Lys Leu Gly Glu Thr Val  *
                785
 
tacgtaaaat ctatgacaca agtttatggt tatttgtctt atgtaagcaa tatttggatt 5465
gatgtctcga gaccatcaac tccatcactg ataagttgat cggatttgta tttctgtccc 5525
ctatttacta attccctttc cagaaataga tcatgaatga ggcagaatat aagtgccaaa 5585
gatgccggct gccgttgacc atagacggat ctctggaaga ccttagcata tcacaggcca 5645
atcttttgac gggacgaaat gggaacttta caaagaacac aatccccttg gaggatgccg 5705
tggaagaaga tttacccaag gtgcctcaga gccgacttaa cctctttaaa gaggtctacc 5765
agaagatgga tcacgatttt accaatgcca gagatgaatt tgttgtgttg aacaagcaca 5825
atgataacag cgacgtcaat gtggagtatg attacgaaga aaacaacact atcagtcgta 5885
gaatcaacac aatgacgaat atcttcaata tcctcagcaa caagtacgaa attgattttc 5945
cggtttgcta cgaatgcgcc acattgctga tggaggaatt gaagaatgag tacgaaaggg 6005
tcaatgctga taaagaagtt tacgcaaagt ttctatccaa gcttcgcaaa caggacgcag 6065
gtacaaatat gaaagaaaga actgctcaac tactggagca attggagaaa actaagcaag 6125
aagagagaga taaagaaaag aagctccaag gcctatatga tgaaagagat agtttggaaa 6185
aggtattagc ttctttagag aatgaaatgg aacagttgaa tattgaagag cagcaaattt 6245
ttgaattaga gaacaaatat gaatatgagt taatggagtt caagaatgag caaagcagaa 6305
tggaagcaat gtatgaggat ggtttgacgc aattagataa tttaagaaaa gtgaacgtct 6365
ttaatgacgc tttcaatatc tcgcatgatg gtcaattcgg cactataaat gggctcaggt 6425
tgggcacgtt agacagtaag agggtttctt ggtatgaaat aaatgctgcg ttgggtcaag 6485
ttgttttgtt actcttcacg ttattgagca gacttgagct tgagctcaaa cattacaaga 6545
tttttcccat tggctcgact tccaagattg aataccaagt tgacccagat tccaaacctg 6605
ttactattaa ctgcttttct tcgggagaac agttactgga taagcttttt cattctaata 6665
aactagatcc tgctatgaac gcaatcctag aaatcactat tcaaattgca gatcatttca 6725
caaaacaaga tccaacaaac gaattgccct acaaaatgga gaacgaaaca atatcaaact 6785
tgaatatcaa accttccaaa cgtaaatcca acgaggaatg gactttggca tgcaaacatc 6845
tgttgaccaa tctcaaatgg ataattgcct tcagtagttc aacgtgaact agtgtattaa 6905
aaaaaagaaa cagaaacttt attggattat aaaactattt atcaagttca aattaacata 6965
gcgacgaaga gaccagctgc ggctaagact gaactaccta gtaccgcttg ggcaccgtta 7025
ccagtttctg tacctgtgcc agtggtacca gtaccagtac cggttccagt gccagttcct 7085
gtgcctgtgc ctgtgcctgt gccggttccg gttccagtgc ctgtgcctgt gcccgtgcct 7145
gttgcagtgg tattagtgaa acctcctgtg ccagttgcag tggtattagt aaatcctcct 7205
gttcctgtgg tgtttgtgag tcctccagtt tcggtcaagt ttccaggaac actaacatca 7265
ggggttgaag tgatctctgg tggcaccgtg gggactgtga cattgacatc atttgtgaag 7325
attggctcca actcagttgt agccttaaca acgcttaatg cgagagttgc accgatcaaa 7385
cttttgaatt gcattttact tttgttactt ctaaaatgag atgaggaaag aaagaagaga 7445
gaagtggaag cactgaaagt gtggtgttat atctgaaaaa ttcattacca atcaaaacgt 7505
cagacgatga tatgtctaag cccgtgcaga aacgtctaga tcttttcaaa cgtaaagtac 7565
ttcccctttt ggcacatcgt ggacttgcta ttccaaatat agacggggac cttttttaga 7625
gtatccccgg gcgcctcgaa ttctggggta tttttttgct atagcatgaa ttggcaatag 7685
ggattgggga caacgtgttt gacagaagac gtgtgtgtcc tgccaaaaag gggtaaaggt 7745
gcatttgcca aggcctgtga atgatctgaa cactagagga aagcaagaag gctgtgtcgt 7805
agtctgtatt ggctgtgttg tcgctgtgtc ggttgcttca aaactttatt cgagtccggt 7865
acgcgtcaat gggtattttt caaaaagttt ctaactccct caatcaactt tggttttggc 7925
cggatatggc atgccagaaa aggaagtttt actcctggcg atgatgttta caaatcaagc 7985
ttagagggag taaccaatgc agataagttt gcgatggcgc tgatctttat gctctcaaca 8045
ccttctcgac tattcagggt catttcgtgg ctttgtattt cgggcacaac tgatcaccga 8105
ggatcaatga aattttcatg cacatcactg atccagtttc tgtcgaattt gcaattccag 8165
ttgattgcag gacccgcgtt ctgcctacac attttctcgt gattgtggaa gtaattctaa 8225
ttgacagtcg atcaccacaa tgacaatctt agttgacctt agattccagt ggaatgcagt 8285
tgaattgtct tttcgtttaa ttagaggaga gtaacggacc agggctcctt tattgtatat 8345
aataattata atttttttca ctatttcacc ttttcgcttg gaatataaaa ttctaattat 8405
aattcaacag gaaatattgt ccaaaccaca tgaagttgtc atg                   8448

<210> 48
<211> 789
<212> PRT
<213> Pichia pastoris

<400> 48
Met Cys Gln Ile Phe Leu Pro Gln Asn Val Thr Arg Cys Ser Val Ser
 1               5                  10                  15      
Leu Leu Thr Met Ser Lys Thr Ser Pro Gln Glu Val Pro Glu Asn Thr
            20                  25                  30          
Thr Glu Leu Lys Ile Ser Lys Gly Glu Leu Arg Pro Phe Ile Val Thr
        35                  40                  45              
Ser Pro Ser Pro Gln Leu Ser Lys Ser Arg Ser Val Thr Ser Thr Lys
    50                  55                  60                  
Glu Lys Leu Ile Leu Ala Ser Leu Phe Ile Phe Ala Met Val Ile Arg
65                  70                  75                  80  
Phe His Asn Val Ala His Pro Asp Ser Val Val Phe Asp Glu Val His
                85                  90                  95      
Phe Gly Gly Phe Ala Arg Lys Tyr Ile Leu Gly Thr Phe Phe Met Asp
            100                 105                 110         
Val His Pro Pro Leu Ala Lys Leu Leu Phe Ala Gly Val Gly Ser Leu
        115                 120                 125             
Gly Gly Tyr Asp Gly Glu Phe Glu Phe Lys Lys Ile Gly Asp Glu Phe
    130                 135                 140                 
Pro Glu Asn Val Pro Tyr Val Leu Met Arg Tyr Leu Pro Ser Gly Met
145                 150                 155                 160 
Gly Val Gly Thr Cys Ile Met Leu Tyr Leu Thr Leu Arg Ala Ser Gly
                165                 170                 175     
Cys Gln Pro Ile Val Cys Ala Leu Thr Thr Ala Leu Leu Ile Ile Glu
            180                 185                 190         
Asn Ala Asn Val Thr Ile Ser Arg Phe Ile Leu Leu Asp Ser Pro Met
        195                 200                 205             
Leu Phe Phe Ile Ala Ser Thr Val Tyr Ser Phe Lys Lys Phe Gln Ile
    210                 215                 220                 
Gln Glu Pro Phe Thr Phe Gln Trp Tyr Lys Thr Leu Ile Ala Thr Gly
225                 230                 235                 240 
Val Ser Leu Gly Leu Ala Ala Ser Ser Lys Trp Val Gly Leu Phe Thr
                245                 250                 255     
Val Ala Trp Ile Gly Leu Ile Thr Ile Trp Asp Leu Trp Phe Ile Ile
            260                 265                 270         
Gly Asp Leu Thr Val Ser Val Lys Lys Ile Phe Gly His Phe Ile Thr
        275                 280                 285             
Arg Ala Val Ala Phe Leu Val Val Pro Thr Leu Ile Tyr Leu Thr Phe
    290                 295                 300                 
Phe Ala Ile His Leu Gln Val Leu Thr Lys Glu Gly Asp Gly Gly Ala
305                 310                 315                 320 
Phe Met Ser Ser Val Phe Arg Ser Thr Leu Glu Gly Asn Ala Val Pro
                325                 330                 335     
Lys Gln Ser Leu Ala Asn Val Gly Leu Gly Ser Leu Val Thr Ile Arg
            340                 345                 350         
His Leu Asn Thr Arg Gly Gly Tyr Leu His Ser His Asn His Leu Tyr
        355                 360                 365             
Glu Gly Gly Ser Gly Gln Gln Gln Val Thr Leu Tyr Pro His Ile Asp
    370                 375                 380                 
Ser Asn Asn Gln Trp Ile Val Gln Asp Tyr Asn Ala Thr Glu Glu Pro
385                 390                 395                 400 
Thr Glu Phe Val Pro Leu Lys Asp Gly Val Lys Ile Arg Leu Asn His
                405                 410                 415     
Lys Leu Thr Ser Arg Arg Leu His Ser His Asn Leu Arg Pro Pro Val
            420                 425                 430         
Thr Glu Gln Asp Trp Gln Asn Glu Val Ser Ala Tyr Gly His Glu Gly
        435                 440                 445             
Phe Gly Gly Asp Ala Asn Asp Asp Phe Val Val Glu Ile Ala Lys Asp
    450                 455                 460                 
Leu Ser Thr Thr Glu Glu Ala Lys Glu Asn Val Arg Ala Ile Gln Thr
465                 470                 475                 480 
Val Phe Arg Leu Arg His Ala Met Thr Gly Cys Tyr Leu Phe Ser His
                485                 490                 495     
Glu Val Lys Leu Pro Lys Trp Ala Tyr Glu Gln Gln Glu Val Thr Cys
            500                 505                 510         
Ala Thr Gln Gly Ile Lys Pro Leu Ser Tyr Trp Tyr Val Glu Thr Asn
        515                 520                 525             
Glu Asn Pro Phe Leu Asp Lys Glu Val Asp Glu Ile Val Ser Tyr Pro
    530                 535                 540                 
Val Pro Thr Phe Phe Gln Lys Val Ala Glu Leu His Ala Arg Met Trp
545                 550                 555                 560 
Lys Ile Asn Lys Gly Leu Thr Asp His His Val Tyr Glu Ser Ser Pro
                565                 570                 575     
Asp Ser Trp Pro Phe Leu Leu Arg Gly Ile Ser Tyr Trp Ser Lys Asn
            580                 585                 590         
His Ser Gln Ile Tyr Phe Ile Gly Asn Ala Val Thr Trp Trp Thr Val
        595                 600                 605             
Thr Ala Ser Ile Ala Leu Phe Ser Val Phe Leu Val Phe Ser Ile Leu
    610                 615                 620                 
Arg Trp Gln Arg Gly Phe Gly Phe Ser Val Asp Pro Thr Val Phe Asn
625                 630                 635                 640 
Phe Asn Val Gln Met Leu His Tyr Ile Leu Gly Trp Val Leu His Tyr
                645                 650                 655     
Leu Pro Ser Phe Leu Met Ala Arg Gln Leu Phe Leu His His Tyr Leu
            660                 665                 670         
Pro Ser Leu Tyr Phe Gly Ile Leu Ala Leu Gly His Val Phe Glu Ile
        675                 680                 685             
Ile His Ser Tyr Val Phe Lys Asn Lys Gln Val Val Ser Tyr Ser Ile
    690                 695                 700                 
Phe Val Leu Phe Phe Ala Val Ala Leu Ser Phe Phe Gln Arg Tyr Ser
705                 710                 715                 720 
Pro Leu Ile Tyr Ala Gly Arg Trp Thr Lys Asp Gln Cys Asn Glu Ser
                725                 730                 735     
Lys Ile Leu Lys Trp Asp Phe Asp Cys Asn Thr Phe Pro Ser His Thr
            740                 745                 750         
Ser Gln Tyr Glu Ile Trp Ala Ser Pro Val Gln Thr Ser Thr Pro Lys
        755                 760                 765             
Glu Gly Thr His Ser Glu Ser Thr Val Gly Glu Pro Asp Val Glu Lys
    770                 775                 780                 
Leu Gly Glu Thr Val
785                 


<210> 49
<211> 8400
<212> DNA
<213> Pichia pastoris

<220> 
<221> CDS            
<222> (3169)...(5394)
<223> Encodes PMT4

<400> 49
tagtaaagaa atcttgcagt ttaattcttc ctcttgtgtt tttagcgatg agacatcggc 60
actcagagtt aagtttgctt gcatctgctc tgataacttt tgctgtgact ctgttgcaat 120
gcttttggta acggtcaatt cgtctatggt ttgttgatac tttgacttta aggcagtaat 180
attgtcctgt agtttatcat tatatgcttc caatgttttg acctttgatg aaatgttttt 240
tcgattaaca gttagttcat cgaaggagag ctccaactct gatacttgca ttcttaaatt 300
atttataatg gtatccttaa cttctagtga tttcgagtgg cttgcctggg cactcttaag 360
ttcttttctc aactgtgcta tggatggctc aagcactaga atttgtttct ctgaatcgaa 420
taattttatt tctagcttct gagcaagctc acaggcgctt actttttcgg aagttagaaa 480
ctttgcttcg ttattcatgg cagacagttc tattcttaat tgcttatttt ctttcctaac 540
ttccaaaatc tccgattcca ggggttcata tctacgggag gaaacctgat tgcatgactt 600
ttcgaacgtt ttttgatcag aaagttgaca gattgtgcca tcagttgacg agacagcctc 660
aaactgagtt gcttccatgt tgcacaaatt atcattgaat tcagccactt ctttctccaa 720
atctccgttt accagctcct tcttctttcc tgaagcaata gatgatgatc gatgaatata 780
gtcctctttc aatgggtttt ggatctcttt gtcccattga caagaagcta tgctccttga 840
atccttcatt gacattgggt atgaaatttt gctaccatct acctttgcac taatttctgt 900
gggcgaattg tgtgttttca gtagatcttc aagtgctttc ttttcgtttt ctatcttcat 960
aagagatatt ctcaatttat ttacggtgtc agtggcagac aaattgttta atgaaacggt 1020
atccagagac tcctgctcca ggtactctga ctgagctaca gggaagggtt tcttgtcgtg 1080
tatggagaac ttctgctcaa gttgggcttt caaggaattc acttggtgat gcaaaagctc 1140
attttcctca ttcaaagaag tattcatatt tttaagttcc tccagctcaa acgtactgcg 1200
gccttccaaa gtgcagattt tatccttaag actcaatttc tcattcttca aactttccat 1260
ctctctattg agcgtttcaa cctggtcagt tttcaacttg agttcttctg aaaatttgat 1320
acttgaactt ttagcaaggg aagcttcatc aaaagtatct tgtagcttgg tttctaaaga 1380
ccagttagaa tcaagtagcc tttgctgctc ttgttccaac tttttggaaa aaattgcctg 1440
gtgagtgatc ttttcagcga gatcctcatt ttctttaact ttttgcttca atagagattt 1500
gagtttttga ttatcagagt tcagcttccg acattcgact aaaaggttgt cactaatacc 1560
tgttaaaaaa tctattttgt tcgtcttttt tttgcttggc gactttagag gtaatgcagg 1620
aagggaagga tgaaattctg actcctggtg ccgatttctc agctttctcg gaagtggggg 1680
tagctgaaat ggtacattgt ggttcgtatc accaagatcc atttttatgt ctttgtccat 1740
tagaaaattc aagaatcttt caaaaaaaat agaaacagaa gatttagtaa acttaggtga 1800
ggtgatataa acctaattgc ctgttttatt ttgatcatgt atgtaaattg tgaaaggtaa 1860
atacgcgaaa cttatgtatg tattgcaaag atgcacaaga cacacaagga ttaatgggct 1920
atttgctcta cattcgcaaa aaatagccag catttatttt ttgaatggat actcaataag 1980
cccatcccta cgcttccata tctttttttt ctttttggta gtaacatgct ccacgaatac 2040
ctcttcacaa gtagattttt taaatgagcg gataaagcgg gggtcccata gttcactagc 2100
aactcctaag tctttgcagc atctcattaa agcattgctc ttacagcctt cagtagcagt 2160
aggaattccc ttctctgaaa aaaaatcttg ctctccgcgt gcaatagaaa ctagtcggcc 2220
ctgtacaatt aaagcatact ccctggttaa agtacctcct ccgaacttgc tcttgttgat 2280
caaagtttct gaccttgggg ccagtcccca gccaccaggg ccaaacgctt tattgaggat 2340
acgacgatac ttaatctctg gaagataaag tagtccatct ggtgtgattt cgacatcttc 2400
gttgctaatt ggttgacata atatgttact actttcatta ctgaaggagc aaatacctag 2460
tccatggaac gaatccgacc aattgattcc atcgccactt gtattagaga ttggggtgtc 2520
gtttaactgt gaagttccaa acaaaattga taaactgctc tcgttcttag cttggccact 2580
ttttggagtc tcaatagtag cgttttggct ctcgtgaatt ttctgcacag agtcggatga 2640
agaaggtgca aatgcttcta gcattgtaga gtcgaccaca tagaaccttt ttaaagagtt 2700
atgaaaataa ctcttggtag ggccaaatac aacccgatat cgtcttagca taagagctgc 2760
ttctttggaa tatcgtttct tgtaagtaat tacgtgttgg ctaaacactt agaagtcagt 2820
cgcgcatgcg gccaaaaaca gactagggat agaagatgaa ctgacaaaaa catcaagaag 2880
gtgaagacat tcattctatg aaaactagtt tttatataaa attatggtct gcatttagag 2940
agcaatgatg taatcaaaca tcaataagtg cttgtcgcat caatatttaa taggtaatca 3000
tggagtattc tagtctaccg ccttaaaaaa agctcactcg atctagtgca gcttgattgt 3060
gtacttcaat agtattccaa cgaccttaac atcttaacac catgtaaatt taagatccac 3120
gtatacgata caatttcttt caatatcaat tctcgttcaa gccaactg atg ata aaa  3177
                                                     Met Ile Lys
                                                      1
 
tca aga aag aga tcg aga aaa gtt tct ttg aac act gaa aag gag ctg   3225
Ser Arg Lys Arg Ser Arg Lys Val Ser Leu Asn Thr Glu Lys Glu Leu
     5                   10                  15
 
aaa aat agc cat att tct ctt gga gat gaa aga tgg tac act gtg ggt   3273
Lys Asn Ser His Ile Ser Leu Gly Asp Glu Arg Trp Tyr Thr Val Gly
 20                  25                  30                  35
 
ctt ctc ttg gtg aca atc aca gct ttc tgt act cga ttc tat gct atc   3321
Leu Leu Leu Val Thr Ile Thr Ala Phe Cys Thr Arg Phe Tyr Ala Ile
                 40                  45                  50
 
aac tat cca gat gag gtt gtt ttt gac gaa gtt cat ttc gga aaa ttt   3369
Asn Tyr Pro Asp Glu Val Val Phe Asp Glu Val His Phe Gly Lys Phe
             55                  60                  65
 
gct agc tac tat cta gag cgt act tat ttt ttt gat ctg cac cct ccg   3417
Ala Ser Tyr Tyr Leu Glu Arg Thr Tyr Phe Phe Asp Leu His Pro Pro
         70                  75                  80
 
ttt gcc aag ctc ctg att gcg ttt gtc ggc ttt tta gct ggg tac aat   3465
Phe Ala Lys Leu Leu Ile Ala Phe Val Gly Phe Leu Ala Gly Tyr Asn
     85                  90                  95
 
ggt gag ttc aag ttt aca act att ggt gaa tct tat atc aaa aac gag   3513
Gly Glu Phe Lys Phe Thr Thr Ile Gly Glu Ser Tyr Ile Lys Asn Glu
100                 105                 110                 115
 
gtt ccc tac gta gtt tac aga tca ttg agc gct gtg caa gga tct tta   3561
Val Pro Tyr Val Val Tyr Arg Ser Leu Ser Ala Val Gln Gly Ser Leu
                120                 125                 130
 
acg gtg cca att gtt tat ttg tgt ctc aaa gaa tgc gga tat aca gtt   3609
Thr Val Pro Ile Val Tyr Leu Cys Leu Lys Glu Cys Gly Tyr Thr Val
            135                 140                 145
 
ttg act tgt gtt ttt ggt gca tgt atc ata ttg ttt gat ggg gcc cac   3657
Leu Thr Cys Val Phe Gly Ala Cys Ile Ile Leu Phe Asp Gly Ala His
        150                 155                 160
 
gtt gct gag act aga cta atc ttg ctg gat gcc acg ttg att ttt ttc   3705
Val Ala Glu Thr Arg Leu Ile Leu Leu Asp Ala Thr Leu Ile Phe Phe
    165                 170                 175
 
gtt tca ttg tcc atc tat agc tat atc aaa ttc aca aaa caa aga tca   3753
Val Ser Leu Ser Ile Tyr Ser Tyr Ile Lys Phe Thr Lys Gln Arg Ser
180                 185                 190                 195
 
gaa cca ttc ggc caa aag tgg tgg aag tgg ctg ttc ttt aca ggg gtg   3801
Glu Pro Phe Gly Gln Lys Trp Trp Lys Trp Leu Phe Phe Thr Gly Val
                200                 205                 210
 
tct tta tct tgc gtc ata agt acc aag tat gtg ggg gtg ttc acc tat   3849
Ser Leu Ser Cys Val Ile Ser Thr Lys Tyr Val Gly Val Phe Thr Tyr
            215                 220                 225
 
ctt aca ata ggc tgt ggt gtc ctg ttt gac tta tgg agt tta ctg gat   3897
Leu Thr Ile Gly Cys Gly Val Leu Phe Asp Leu Trp Ser Leu Leu Asp
        230                 235                 240
 
tat aaa aag gga cat tcc ttg gca tat gtt ggt aaa cac ttt gct gca   3945
Tyr Lys Lys Gly His Ser Leu Ala Tyr Val Gly Lys His Phe Ala Ala
    245                 250                 255
 
cga ttt ttc ctt cta ata ctg gtc cct ttc ttg ata tat ctc aat tgg   3993
Arg Phe Phe Leu Leu Ile Leu Val Pro Phe Leu Ile Tyr Leu Asn Trp
260                 265                 270                 275
 
ttt tat gtt cat ttc gct att cta agc aag tct ggc cca gga gac agt   4041
Phe Tyr Val His Phe Ala Ile Leu Ser Lys Ser Gly Pro Gly Asp Ser
                280                 285                 290
 
ttt atg agc tct gaa ttc cag gag act ctc gga gat tct cct ctt gca   4089
Phe Met Ser Ser Glu Phe Gln Glu Thr Leu Gly Asp Ser Pro Leu Ala
            295                 300                 305
 
gct ttc gca aag gaa gtt cac ttt aac gac ata atc aca ata aag cat   4137
Ala Phe Ala Lys Glu Val His Phe Asn Asp Ile Ile Thr Ile Lys His
        310                 315                 320
 
aaa gag act gat gcc atg ttg cac tca cac ttg gca aac tac ccc ctc   4185
Lys Glu Thr Asp Ala Met Leu His Ser His Leu Ala Asn Tyr Pro Leu
    325                 330                 335
 
cgt tac gag gac ggg agg gta tca tct caa ggt caa caa gtt aca gca   4233
Arg Tyr Glu Asp Gly Arg Val Ser Ser Gln Gly Gln Gln Val Thr Ala
340                 345                 350                 355
 
tac tct gga gag gac cca aac aat aat tgg cag att att tct ccc gaa   4281
Tyr Ser Gly Glu Asp Pro Asn Asn Asn Trp Gln Ile Ile Ser Pro Glu
                360                 365                 370
 
gga ctt act ggc gtt gta act cag ggc gat gtc gtt aga ctg aga cac   4329
Gly Leu Thr Gly Val Val Thr Gln Gly Asp Val Val Arg Leu Arg His
            375                 380                 385
 
gtt ggg aca gat ggc tat cta ctg acg cat gat gtt gcg tct cct ttc   4377
Val Gly Thr Asp Gly Tyr Leu Leu Thr His Asp Val Ala Ser Pro Phe
        390                 395                 400
 
tat cca act aac gag gag ttt act gta gtg gga cag gag aaa gct act   4425
Tyr Pro Thr Asn Glu Glu Phe Thr Val Val Gly Gln Glu Lys Ala Thr
    405                 410                 415
 
caa cgc tgg aac gaa aca ctt ttt aga att gat ccc tat gac aag aag   4473
Gln Arg Trp Asn Glu Thr Leu Phe Arg Ile Asp Pro Tyr Asp Lys Lys
420                 425                 430                 435
 
aaa acc cgt cct ttg aag tcg aaa gct tca ttt ttc aaa ctc att cat   4521
Lys Thr Arg Pro Leu Lys Ser Lys Ala Ser Phe Phe Lys Leu Ile His
                440                 445                 450
 
gtt cct acg gtt gtg gcc atg tgg act cat aat gac cag ctt ctt cct   4569
Val Pro Thr Val Val Ala Met Trp Thr His Asn Asp Gln Leu Leu Pro
            455                 460                 465
 
gat tgg ggt ttc aac caa caa gaa gtc aat ggt aat aag aag ctt gct   4617
Asp Trp Gly Phe Asn Gln Gln Glu Val Asn Gly Asn Lys Lys Leu Ala
        470                 475                 480
 
gat gaa tca aac tta tgg gtt gta gac aat atc gtc gat att gca gag   4665
Asp Glu Ser Asn Leu Trp Val Val Asp Asn Ile Val Asp Ile Ala Glu
    485                 490                 495
 
gac gat cca agg aaa cac tac gtt cca aag gaa gtg aaa aat ttg cca   4713
Asp Asp Pro Arg Lys His Tyr Val Pro Lys Glu Val Lys Asn Leu Pro
500                 505                 510                 515
 
ttt ttg acc aag tgg ttg gaa tta caa aga ctt atg ttt att cag aat   4761
Phe Leu Thr Lys Trp Leu Glu Leu Gln Arg Leu Met Phe Ile Gln Asn
                520                 525                 530
 
aac aag ttg agc tca gat cat cca ttt gcg tct gac cct ata tct tgg   4809
Asn Lys Leu Ser Ser Asp His Pro Phe Ala Ser Asp Pro Ile Ser Trp
            535                 540                 545
 
cct ttt tca ctt agt ggg gtt tca ttt tgg aca aac aac gag tca cgc   4857
Pro Phe Ser Leu Ser Gly Val Ser Phe Trp Thr Asn Asn Glu Ser Arg
        550                 555                 560
 
aaa cag atc tat ttt gtc gga aat att cct gga tgg tgg atg gag gtt   4905
Lys Gln Ile Tyr Phe Val Gly Asn Ile Pro Gly Trp Trp Met Glu Val
    565                 570                 575
 
gca gca ttg gga tcc ttt cta gga ctc gtg ttt gca gat cag ttc acg   4953
Ala Ala Leu Gly Ser Phe Leu Gly Leu Val Phe Ala Asp Gln Phe Thr
580                 585                 590                 595
 
aga aga aga aac agt ctt gtt ttg acc aat agc gcc agg tct cgg tta   5001
Arg Arg Arg Asn Ser Leu Val Leu Thr Asn Ser Ala Arg Ser Arg Leu
                600                 605                 610
 
tac aat aat ttg ggg ttc ttc ttt gta ggc tgg tgt tgt cat tac cta   5049
Tyr Asn Asn Leu Gly Phe Phe Phe Val Gly Trp Cys Cys His Tyr Leu
            615                 620                 625
 
ccc ttt ttc cta atg agc cgt caa aaa ttt ttg cac cat tac tta cct   5097
Pro Phe Phe Leu Met Ser Arg Gln Lys Phe Leu His His Tyr Leu Pro
        630                 635                 640
 
gca cat tta ata gca gcc atg ttc act gct ggt ttc ttg gaa ttt att   5145
Ala His Leu Ile Ala Ala Met Phe Thr Ala Gly Phe Leu Glu Phe Ile
    645                 650                 655
 
ttt act gac aac aga act gaa gaa ttc aag gat cag aaa act tca tgt   5193
Phe Thr Asp Asn Arg Thr Glu Glu Phe Lys Asp Gln Lys Thr Ser Cys
660                 665                 670                 675
 
gaa cct aac tct aat tct tca aag ccg aaa gag caa ttg att ctg tgg   5241
Glu Pro Asn Ser Asn Ser Ser Lys Pro Lys Glu Gln Leu Ile Leu Trp
                680                 685                 690
 
tta agt ttc tcg tcc ttt gtc gct ttg cta cta agc atc att gtt tgg   5289
Leu Ser Phe Ser Ser Phe Val Ala Leu Leu Leu Ser Ile Ile Val Trp
            695                 700                 705
 
act ttc ttc ttt ttt gct cct cta aca tat ggt aat act gcg ctt tcg   5337
Thr Phe Phe Phe Phe Ala Pro Leu Thr Tyr Gly Asn Thr Ala Leu Ser
        710                 715                 720
 
gcg gag gag gtt cag cag cga caa tgg tta gat atg aag ctc caa ttc   5385
Ala Glu Glu Val Gln Gln Arg Gln Trp Leu Asp Met Lys Leu Gln Phe
    725                 730                 735
 
gcc aag taa gagtatacaa tgtgtagttc aacgcaaagg aaattctaac           5434
Ala Lys  *
740
 
tttctgtgca atctggtgac aatttctaaa taactatcac aattggaaga agagattatc 5494
ccaaatctta tcaaaaaatc gatgattgcc agtgcacaat taggcttgaa tttttcttgc 5554
agcaacgaag agattacttc agtgatgttc attagcctga aatcttcact ttcgtggtct 5614
atcggattag gaattagacc ttgtttcatc ggcaggtcgt atatgtattc cacttctggt 5674
tgaataaaat cttcgggtgg tttgtttctg aacatatatg agatggctcc cactggactg 5734
atatattgcg aaacatagtc ctcattcaac cctgcctcct cgtaacattc tttcaggcaa 5794
gtttgcaaag tgccattagg atattccaag cctcctgcca cagtattatc taacataccg 5854
ggaaatgttg gtttgtgtct gcttctccta ggtatccaaa gttgaatact gttaggatcg 5914
gcagaatttt gcaaatatcc attgatatga actccataag taacaactcc caaaatatta 5974
gaaaaagccc tttccaccaa catgtacatc ttatggttat cgcagtaaac tgcaaaaagc 6034
tcatttctcc aaccgctaag ggtttcaaag agacgctgat ctctccaacg ctgagctatc 6094
tttgcaaaca tctgcgttct tttattttcg gtatccagac taggaattat cttgacttcg 6154
tgtttttcat tatttactat cacagcctgt gtttcgaact caaattgttt tgccaccttg 6214
ggaattatat accctagtaa gatcccatca tgcgataaga atttatacac agatacttca 6274
aattcatgaa aagatggctc atctttatga ggaacagaat caacagatct gactagatca 6334
atatatggca ttggttgatt ttattcaatg gttatctatc tcaaacatgc tataaaaata 6394
aggtaattcc tttatggtgt tagggtgtta tagtttttgc gtagaaaata attgtcatca 6454
tttttgggca acctatgaaa caactactca gagaagttga gacatctctt ttgacaaatg 6514
aaaccgaaat atcccctgcc cttaagctat taattactca gttaaataaa tcaacccatg 6574
aagataaatc aacagaaaga aaaacgtttt ggctagcatt agacaattta aggcaaaaaa 6634
tcggtctaca atcccaatca catgtccttt tctttctaca tctttttgaa gagctagctc 6694
caactttaga aaatgagaaa atatttttaa cctggattac ttcttttttg aagttagcaa 6754
ttaatagtgc aggggtacca cattgtgtgg tgaacgagtc aaggagaatt ataatgaatt 6814
tattattgcc ctcaaaagct acaaacaccg aatacaattt gttaaagaat tctgctgcag 6874
gcattcaatt acttgtgcaa gtgtatttgc taaaaactga tttagttgtt gattccactt 6934
ctagtagtcc ccaggagtat gaagagaggg ttagattcat aaagaaaaac tgcagggatt 6994
tactacaagg tcttgattta aataatcaag tactagaggc tatcagcaaa gaatttacgg 7054
atcctcacta ccgcttcgag tgcttcgtac ttttgtcctc attaatgtcg tcatcagcct 7114
tgttgtacca gataatgcaa acaactttgt ggcataatat acttttgtct atattgatag 7174
ataaaagtaa cagtgtggtt gagtcaggaa tcaaggttct cagtatggtt ttgccccacg 7234
tctgtgatgt aatagcggat tatctaccga ccattatggc gattttaagt aaaggtctgg 7294
ggggtgttga aattgatgat gagtcaccat taccatcaaa ttggaaagta ttgaatgatc 7354
aggatcctga aattattggt ccagcatttg ttagctataa acaactgttc actgtattat 7414
acggcctgtt ccctcttagt ttaacatcat ttattcgcag tccatctaca tatatcgact 7474
ctaacaagat tatagacgat ctcaagcttc agttgcttga aactaaagtg aagtcaaagt 7534
gtcaggactt gctaaagtgt tttattgttc atccaaatta ttttatatat tcttcccagg 7594
aggaagaaat ttttgatact tcaaggtggg acaaaatgca ctccccgaac gagatagcag 7654
cattttgtta tcaattggaa ttccgtggga catcgaagga gaatgccttt gatatgaggg 7714
tagatgacct tttggaaggt catcgatatc tatatttgaa agatatgaag gatgcgcaga 7774
aagagagggc taaaaaatgt gaaaattcta ttatctcact cgaaagttca tctgatagta 7834
agtcagtttc acaatacgac gaagactcga cgaaagaaac cacttgcagg catgtttcgt 7894
tttatttaag agagatcctt ttggcaaaaa atgaattgga cttcacgcta catatcaatc 7954
aggtacttgg agccgagtgt gagcttttga aaaaaaaatt gaacgaaatg gataccctac 8014
gagatcaaaa caggttttta gctgacataa acgaaggttt acgaatacag caatctaagg 8074
cgagtgagca aattacggaa ttgctcaaag aaaaagagcg ttctcaaaat gatttcaact 8134
ctctggttac tcatatgctt aaacaatcta acgaattaaa agaaagggag tcgaaactag 8194
tcgagattca tcaatcaaat gatgcagaga taggagattt aaattatagg ttggaaaaac 8254
tgtgcaacct tatacaaccc aaagaattag aagtggaact gctcaagaag aagttgcgtg 8314
tagcatcgat ccttttttcg caagataaat caaaatcttc aagcaagaca tctctagcac 8374
atttgcacca ggcaggcgac gcaact                                      8400

<210> 50
<211> 741
<212> PRT
<213> Pichia pastoris

<400> 50
Met Ile Lys Ser Arg Lys Arg Ser Arg Lys Val Ser Leu Asn Thr Glu
 1               5                  10                  15      
Lys Glu Leu Lys Asn Ser His Ile Ser Leu Gly Asp Glu Arg Trp Tyr
            20                  25                  30          
Thr Val Gly Leu Leu Leu Val Thr Ile Thr Ala Phe Cys Thr Arg Phe
        35                  40                  45              
Tyr Ala Ile Asn Tyr Pro Asp Glu Val Val Phe Asp Glu Val His Phe
    50                  55                  60                  
Gly Lys Phe Ala Ser Tyr Tyr Leu Glu Arg Thr Tyr Phe Phe Asp Leu
65                  70                  75                  80  
His Pro Pro Phe Ala Lys Leu Leu Ile Ala Phe Val Gly Phe Leu Ala
                85                  90                  95      
Gly Tyr Asn Gly Glu Phe Lys Phe Thr Thr Ile Gly Glu Ser Tyr Ile
            100                 105                 110         
Lys Asn Glu Val Pro Tyr Val Val Tyr Arg Ser Leu Ser Ala Val Gln
        115                 120                 125             
Gly Ser Leu Thr Val Pro Ile Val Tyr Leu Cys Leu Lys Glu Cys Gly
    130                 135                 140                 
Tyr Thr Val Leu Thr Cys Val Phe Gly Ala Cys Ile Ile Leu Phe Asp
145                 150                 155                 160 
Gly Ala His Val Ala Glu Thr Arg Leu Ile Leu Leu Asp Ala Thr Leu
                165                 170                 175     
Ile Phe Phe Val Ser Leu Ser Ile Tyr Ser Tyr Ile Lys Phe Thr Lys
            180                 185                 190         
Gln Arg Ser Glu Pro Phe Gly Gln Lys Trp Trp Lys Trp Leu Phe Phe
        195                 200                 205             
Thr Gly Val Ser Leu Ser Cys Val Ile Ser Thr Lys Tyr Val Gly Val
    210                 215                 220                 
Phe Thr Tyr Leu Thr Ile Gly Cys Gly Val Leu Phe Asp Leu Trp Ser
225                 230                 235                 240 
Leu Leu Asp Tyr Lys Lys Gly His Ser Leu Ala Tyr Val Gly Lys His
                245                 250                 255     
Phe Ala Ala Arg Phe Phe Leu Leu Ile Leu Val Pro Phe Leu Ile Tyr
            260                 265                 270         
Leu Asn Trp Phe Tyr Val His Phe Ala Ile Leu Ser Lys Ser Gly Pro
        275                 280                 285             
Gly Asp Ser Phe Met Ser Ser Glu Phe Gln Glu Thr Leu Gly Asp Ser
    290                 295                 300                 
Pro Leu Ala Ala Phe Ala Lys Glu Val His Phe Asn Asp Ile Ile Thr
305                 310                 315                 320 
Ile Lys His Lys Glu Thr Asp Ala Met Leu His Ser His Leu Ala Asn
                325                 330                 335     
Tyr Pro Leu Arg Tyr Glu Asp Gly Arg Val Ser Ser Gln Gly Gln Gln
            340                 345                 350         
Val Thr Ala Tyr Ser Gly Glu Asp Pro Asn Asn Asn Trp Gln Ile Ile
        355                 360                 365             
Ser Pro Glu Gly Leu Thr Gly Val Val Thr Gln Gly Asp Val Val Arg
    370                 375                 380                 
Leu Arg His Val Gly Thr Asp Gly Tyr Leu Leu Thr His Asp Val Ala
385                 390                 395                 400 
Ser Pro Phe Tyr Pro Thr Asn Glu Glu Phe Thr Val Val Gly Gln Glu
                405                 410                 415     
Lys Ala Thr Gln Arg Trp Asn Glu Thr Leu Phe Arg Ile Asp Pro Tyr
            420                 425                 430         
Asp Lys Lys Lys Thr Arg Pro Leu Lys Ser Lys Ala Ser Phe Phe Lys
        435                 440                 445             
Leu Ile His Val Pro Thr Val Val Ala Met Trp Thr His Asn Asp Gln
    450                 455                 460                 
Leu Leu Pro Asp Trp Gly Phe Asn Gln Gln Glu Val Asn Gly Asn Lys
465                 470                 475                 480 
Lys Leu Ala Asp Glu Ser Asn Leu Trp Val Val Asp Asn Ile Val Asp
                485                 490                 495     
Ile Ala Glu Asp Asp Pro Arg Lys His Tyr Val Pro Lys Glu Val Lys
            500                 505                 510         
Asn Leu Pro Phe Leu Thr Lys Trp Leu Glu Leu Gln Arg Leu Met Phe
        515                 520                 525             
Ile Gln Asn Asn Lys Leu Ser Ser Asp His Pro Phe Ala Ser Asp Pro
    530                 535                 540                 
Ile Ser Trp Pro Phe Ser Leu Ser Gly Val Ser Phe Trp Thr Asn Asn
545                 550                 555                 560 
Glu Ser Arg Lys Gln Ile Tyr Phe Val Gly Asn Ile Pro Gly Trp Trp
                565                 570                 575     
Met Glu Val Ala Ala Leu Gly Ser Phe Leu Gly Leu Val Phe Ala Asp
            580                 585                 590         
Gln Phe Thr Arg Arg Arg Asn Ser Leu Val Leu Thr Asn Ser Ala Arg
        595                 600                 605             
Ser Arg Leu Tyr Asn Asn Leu Gly Phe Phe Phe Val Gly Trp Cys Cys
    610                 615                 620                 
His Tyr Leu Pro Phe Phe Leu Met Ser Arg Gln Lys Phe Leu His His
625                 630                 635                 640 
Tyr Leu Pro Ala His Leu Ile Ala Ala Met Phe Thr Ala Gly Phe Leu
                645                 650                 655     
Glu Phe Ile Phe Thr Asp Asn Arg Thr Glu Glu Phe Lys Asp Gln Lys
            660                 665                 670         
Thr Ser Cys Glu Pro Asn Ser Asn Ser Ser Lys Pro Lys Glu Gln Leu
        675                 680                 685             
Ile Leu Trp Leu Ser Phe Ser Ser Phe Val Ala Leu Leu Leu Ser Ile
    690                 695                 700                 
Ile Val Trp Thr Phe Phe Phe Phe Ala Pro Leu Thr Tyr Gly Asn Thr
705                 710                 715                 720 
Ala Leu Ser Ala Glu Glu Val Gln Gln Arg Gln Trp Leu Asp Met Lys
                725                 730                 735     
Leu Gln Phe Ala Lys
            740     


<210> 51
<211> 1380
<212> DNA
<213> Artificial Sequence

<220> 
<223> anti-DKK1 Heavy chain (VH + IgG2m4) with
      alpha-amylase leader

<400> 51
acgatggtcg cttggtggtc tttgtttctg tacggtcttc aggtcgctgc acctgctttg 60
gctgaggttc agttggttca atctggtgct gaggttaaga aacctggtgc ttccgttaag 120
gtttcctgta aggcttccgg ttacactttc actgactact acatccactg ggttagacaa 180
gctccaggtc aaggattgga atggatggga tggattcact ctaactccgg tgctactact 240
tacgctcaga agttccaggc tagagttact atgtccagag acacttcttc ttccactgct 300
tacatggaat tgtccagatt ggaatccgat gacactgcta tgtacttttg ttccagagag 360
gactactggg gacagggaac tttggttact gtttcctccg cttctactaa agggccctct 420
gtttttccat tggctccatg ttctagatcc acttccgaat ccactgctgc tttgggatgt 480
ttggttaagg actacttccc agagccagtt actgtttctt ggaactccgg tgctttgact 540
tctggtgttc acactttccc agctgttttg caatcttccg gtttgtactc cttgtcctcc 600
gttgttactg ttacttcctc caacttcggt actcagactt acacttgtaa cgttgaccac 660
aagccatcca acactaaggt tgacaagact gttgagagaa agtgttgtgt tgagtgtcca 720
ccatgtccag ctccaccagt tgctggtcca tccgtttttt tgttcccacc aaagccaaag 780
gacactttga tgatctccag aactccagag gttacatgtg ttgttgttga cgtttcccaa 840
gaggacccag aggttcaatt caactggtac gttgacggtg ttgaagttca caacgctaag 900
actaagccaa gagaagagca gttcaactcc actttcagag ttgtttccgt tttgactgtt 960
ttgcaccagg attggttgaa cggtaaagaa tacaagtgta aggtttccaa caagggattg 1020
ccatcctcca tcgaaaagac tatctccaag actaagggac aaccaagaga gccacaggtt 1080
tacactttgc caccatccag agaagagatg actaagaacc aggtttcctt gacttgtttg 1140
gttaaaggat tctacccatc cgacattgct gttgagtggg aatctaacgg tcaaccagag 1200
aacaactaca agactactcc accaatgttg gattctgacg gttccttctt cttgtactcc 1260
aagttgactg ttgacaagtc cagatggcaa cagggtaacg ttttctcctg ttccgttatg 1320
catgaggctt tgcacaacca ctacactcaa aagtccttgt ctttgtcccc tggtaagtaa 1380


<210> 52
<211> 438
<212> PRT
<213> Artificial Sequence

<220> 
<223> anti-DKK1 Heavy chain (VH + IgG2m4)

<400> 52
Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr
            20                  25                  30          
Tyr Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met
        35                  40                  45              
Gly Trp Ile His Ser Asn Ser Gly Ala Thr Thr Tyr Ala Gln Lys Phe
    50                  55                  60                  
Gln Ala Arg Val Thr Met Ser Arg Asp Thr Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80  
Met Glu Leu Ser Arg Leu Glu Ser Asp Asp Thr Ala Met Tyr Phe Cys
                85                  90                  95      
Ser Arg Glu Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
            100                 105                 110         
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg
        115                 120                 125             
Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
    130                 135                 140                 
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
145                 150                 155                 160 
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
                165                 170                 175     
Leu Ser Ser Val Val Thr Val Thr Ser Ser Asn Phe Gly Thr Gln Thr
            180                 185                 190         
Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys
        195                 200                 205             
Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro
    210                 215                 220                 
Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
225                 230                 235                 240 
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
                245                 250                 255     
Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly
            260                 265                 270         
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn
        275                 280                 285             
Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
    290                 295                 300                 
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro
305                 310                 315                 320 
Ser Ser Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu
                325                 330                 335     
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn
            340                 345                 350         
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
        355                 360                 365             
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
    370                 375                 380                 
Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
385                 390                 395                 400 
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
                405                 410                 415     
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
            420                 425                 430         
Ser Leu Ser Pro Gly Lys
        435             


<210> 53
<211> 717
<212> DNA
<213> Artificial Sequence

<220> 
<223> aanti-DKK1 Light chain (VL + lambda constant
      regions) with alpha-amylase leader

<400> 53
acgatggtcg cttggtggtc tttgtttctg tacggtcttc aggtcgctgc acctgctttg 60
gctcagtccg ttttgacaca accaccatct gtttctggtg ctccaggaca gagagttact 120
atctcctgta ctggttcctc ttccaacatt ggtgctggtt acgatgttca ctggtatcaa 180
cagttgccag gtactgctcc aaagttgttg atctacggtt actccaacag accatctggt 240
gttccagaca gattctctgg ttctaagtct ggtgcttctg cttccttggc tatcactgga 300
ttgagaccag atgacgaggc tgactactac tgtcaatcct acgacaactc cttgtcctct 360
tacgttttcg gtggtggtac tcagttgact gttttgtccc agccaaaggc taatccaact 420
gttactttgt tcccaccatc ttccgaagaa ctgcaggcta ataaggctac tttggtttgt 480
ttgatctccg acttctaccc aggtgctgtt actgttgctt ggaaggctga tggttctcca 540
gttaaggctg gtgttgagac tactaagcca tccaagcagt ccaataacaa gtacgctgct 600
agctcttact tgtccttgac accagaacaa tggaagtccc acagatccta ctcttgtcag 660
gttacacacg agggttctac tgttgaaaag actgttgctc caactgagtg ttcctaa    717

<210> 54
<211> 217
<212> PRT
<213> Artificial Sequence

<220> 
<223> aanti-DKK1 Light chain (VL + lambda constant
      regions)

<400> 54
Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Gly Ala Pro Gly Gln
 1               5                  10                  15      
Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ala Gly
            20                  25                  30          
Tyr Asp Val His Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu
        35                  40                  45              
Leu Ile Tyr Gly Tyr Ser Asn Arg Pro Ser Gly Val Pro Asp Arg Phe
    50                  55                  60                  
Ser Gly Ser Lys Ser Gly Ala Ser Ala Ser Leu Ala Ile Thr Gly Leu
65                  70                  75                  80  
Arg Pro Asp Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Asn Ser
                85                  90                  95      
Leu Ser Ser Tyr Val Phe Gly Gly Gly Thr Gln Leu Thr Val Leu Ser
            100                 105                 110         
Gln Pro Lys Ala Asn Pro Thr Val Thr Leu Phe Pro Pro Ser Ser Glu
        115                 120                 125             
Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe
    130                 135                 140                 
Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Gly Ser Pro Val
145                 150                 155                 160 
Lys Ala Gly Val Glu Thr Thr Lys Pro Ser Lys Gln Ser Asn Asn Lys
                165                 170                 175     
Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser
            180                 185                 190         
His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu
        195                 200                 205             
Lys Thr Val Ala Pro Thr Glu Cys Ser
    210                 215         


<210> 55
<211> 1908
<212> DNA
<213> Artificial Sequence

<220> 
<223> encodes human BIP

<400> 55
gaggaagagg acaagaaaga ggatgttggt actgttgtcg gtatcgactt gggtactacc 60
tactcctgtg tcggtgtttt caagaacggt agagtggaga ttatcgccaa cgaccagggt 120
aacagaatta ctccatccta cgttgctttt accccagaag gagagagatt gatcggagac 180
gctgctaaga accaattgac ctccaaccca gagaacactg ttttcgacgc caagagactg 240
attggtagaa cttggaacga cccatccgtt caacaagaca tcaagttctt gcccttcaag 300
gtcgtcgaga agaaaaccaa gccatacatc caggttgaca tcggtggtgg tcaaactaag 360
actttcgctc cagaggaaat ctccgctatg gtcctgacta agatgaaaga gactgccgag 420
gcttacttgg gtaaaaaggt tacccacgct gttgttactg ttccagctta cttcaacgac 480
gctcagagac aagctactaa ggacgctggt actatcgctg gactgaacgt gatgagaatc 540
atcaacgagc caactgctgc tgctattgcc tacggattgg acaagagaga gggagagaag 600
aacatcttgg ttttcgactt gggtggtggt actttcgacg tttccttgtt gaccatcgac 660
aacggtgttt tcgaagttgt tgctaccaac ggtgatactc acttgggtgg agaggacttc 720
gatcagagag tgatggaaca cttcatcaag ctgtacaaga agaaaaccgg aaaggacgtt 780
agaaaggaca acagagccgt tcagaagttg agaagagagg ttgagaaggc taaggctttg 840
tcctcccaac accaagctag aatcgagatc gaatccttct acgagggtga agatttctcc 900
gagaccttga ctagagccaa gttcgaagag ctgaacatgg acctgttcag atccactatg 960
aagccagttc agaaggtttt ggaggattcc gacttgaaga agtccgacat cgacgagatt 1020
gttttggttg gtggttccac cagaatccca aagatccagc agctggtcaa agagttcttc 1080
aacggtaaag agccatccag aggtattaac ccagatgagg ctgttgctta cggtgctgct 1140
gttcaagctg gtgttttgtc tggtgaccag gacactggtg acttggtttt gttgcatgtt 1200
tgcccattga ctttgggtat cgagactgtt ggtggtgtta tgaccaagtt gatcccatcc 1260
aacactgttg ttcccaccaa gaactcccaa attttctcca ctgcttccga caaccagcca 1320
accgttacta ttaaggtcta cgaaggtgaa agaccattga ccaaggacaa ccacttgttg 1380
ggaactttcg acttgactgg tattccacct gctccaagag gtgttccaca aatcgaggtt 1440
accttcgaga tcgacgtcaa cggtatcttg agagttactg ccgaggataa gggaaccggt 1500
aacaagaaca agatcaccat caccaacgac caaaacagat tgacccccga agagatcgaa 1560
agaatggtca acgatgctga gaagttcgcc gaagaggata agaagctgaa agagagaatc 1620
gacaccagaa acgagttgga atcctacgct tactccttga agaaccagat cggtgacaaa 1680
gaaaagttgg gtggaaagct gtcatccgaa gataaagaaa ctatggaaaa ggccgtcgaa 1740
gaaaagattg agtggctgga atctcaccaa gatgctgaca tcgaggactt caaggccaag 1800
aagaaagagt tggaagagat cgtccagcca atcatttcta agttgtacgg ttctgctggt 1860
ccaccaccaa ctggtgaaga agatactgcc gagcacgacg agttgtag              1908

<210> 56
<211> 635
<212> PRT
<213> Artificial Sequence

<220> 
<223> human BIP without leader

<400> 56
Glu Glu Glu Asp Lys Lys Glu Asp Val Gly Thr Val Val Gly Ile Asp
 1               5                  10                  15      
Leu Gly Thr Thr Tyr Ser Cys Val Gly Val Phe Lys Asn Gly Arg Val
            20                  25                  30          
Glu Ile Ile Ala Asn Asp Gln Gly Asn Arg Ile Thr Pro Ser Tyr Val
        35                  40                  45              
Ala Phe Thr Pro Glu Gly Glu Arg Leu Ile Gly Asp Ala Ala Lys Asn
    50                  55                  60                  
Gln Leu Thr Ser Asn Pro Glu Asn Thr Val Phe Asp Ala Lys Arg Leu
65                  70                  75                  80  
Ile Gly Arg Thr Trp Asn Asp Pro Ser Val Gln Gln Asp Ile Lys Phe
                85                  90                  95      
Leu Pro Phe Lys Val Val Glu Lys Lys Thr Lys Pro Tyr Ile Gln Val
            100                 105                 110         
Asp Ile Gly Gly Gly Gln Thr Lys Thr Phe Ala Pro Glu Glu Ile Ser
        115                 120                 125             
Ala Met Val Leu Thr Lys Met Lys Glu Thr Ala Glu Ala Tyr Leu Gly
    130                 135                 140                 
Lys Lys Val Thr His Ala Val Val Thr Val Pro Ala Tyr Phe Asn Asp
145                 150                 155                 160 
Ala Gln Arg Gln Ala Thr Lys Asp Ala Gly Thr Ile Ala Gly Leu Asn
                165                 170                 175     
Val Met Arg Ile Ile Asn Glu Pro Thr Ala Ala Ala Ile Ala Tyr Gly
            180                 185                 190         
Leu Asp Lys Arg Glu Gly Glu Lys Asn Ile Leu Val Phe Asp Leu Gly
        195                 200                 205             
Gly Gly Thr Phe Asp Val Ser Leu Leu Thr Ile Asp Asn Gly Val Phe
    210                 215                 220                 
Glu Val Val Ala Thr Asn Gly Asp Thr His Leu Gly Gly Glu Asp Phe
225                 230                 235                 240 
Asp Gln Arg Val Met Glu His Phe Ile Lys Leu Tyr Lys Lys Lys Thr
                245                 250                 255     
Gly Lys Asp Val Arg Lys Asp Asn Arg Ala Val Gln Lys Leu Arg Arg
            260                 265                 270         
Glu Val Glu Lys Ala Lys Ala Leu Ser Ser Gln His Gln Ala Arg Ile
        275                 280                 285             
Glu Ile Glu Ser Phe Tyr Glu Gly Glu Asp Phe Ser Glu Thr Leu Thr
    290                 295                 300                 
Arg Ala Lys Phe Glu Glu Leu Asn Met Asp Leu Phe Arg Ser Thr Met
305                 310                 315                 320 
Lys Pro Val Gln Lys Val Leu Glu Asp Ser Asp Leu Lys Lys Ser Asp
                325                 330                 335     
Ile Asp Glu Ile Val Leu Val Gly Gly Ser Thr Arg Ile Pro Lys Ile
            340                 345                 350         
Gln Gln Leu Val Lys Glu Phe Phe Asn Gly Lys Glu Pro Ser Arg Gly
        355                 360                 365             
Ile Asn Pro Asp Glu Ala Val Ala Tyr Gly Ala Ala Val Gln Ala Gly
    370                 375                 380                 
Val Leu Ser Gly Asp Gln Asp Thr Gly Asp Leu Val Leu Leu His Val
385                 390                 395                 400 
Cys Pro Leu Thr Leu Gly Ile Glu Thr Val Gly Gly Val Met Thr Lys
                405                 410                 415     
Leu Ile Pro Ser Asn Thr Val Val Pro Thr Lys Asn Ser Gln Ile Phe
            420                 425                 430         
Ser Thr Ala Ser Asp Asn Gln Pro Thr Val Thr Ile Lys Val Tyr Glu
        435                 440                 445             
Gly Glu Arg Pro Leu Thr Lys Asp Asn His Leu Leu Gly Thr Phe Asp
    450                 455                 460                 
Leu Thr Gly Ile Pro Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val
465                 470                 475                 480 
Thr Phe Glu Ile Asp Val Asn Gly Ile Leu Arg Val Thr Ala Glu Asp
                485                 490                 495     
Lys Gly Thr Gly Asn Lys Asn Lys Ile Thr Ile Thr Asn Asp Gln Asn
            500                 505                 510         
Arg Leu Thr Pro Glu Glu Ile Glu Arg Met Val Asn Asp Ala Glu Lys
        515                 520                 525             
Phe Ala Glu Glu Asp Lys Lys Leu Lys Glu Arg Ile Asp Thr Arg Asn
    530                 535                 540                 
Glu Leu Glu Ser Tyr Ala Tyr Ser Leu Lys Asn Gln Ile Gly Asp Lys
545                 550                 555                 560 
Glu Lys Leu Gly Gly Lys Leu Ser Ser Glu Asp Lys Glu Thr Met Glu
                565                 570                 575     
Lys Ala Val Glu Glu Lys Ile Glu Trp Leu Glu Ser His Gln Asp Ala
            580                 585                 590         
Asp Ile Glu Asp Phe Lys Ala Lys Lys Lys Glu Leu Glu Glu Ile Val
        595                 600                 605             
Gln Pro Ile Ile Ser Lys Leu Tyr Gly Ser Ala Gly Pro Pro Pro Thr
    610                 615                 620                 
Gly Glu Glu Asp Thr Ala Glu His Asp Glu Leu
625                 630                 635 


<210> 57
<211> 1896
<212> DNA
<213> Artificial Sequence

<220> 
<223> chimeric BIP

<400> 57
gacgatgtcg aatcttatgg aacagtgatt ggtatcgatt tgggtaccac gtactcttgt 60
gtcggtgtga tgaagtcggg tcgtgtagaa attcttgcta atgaccaagg taacagaatc 120
actccttcct acgttagttt cactgaagac gagagactgg ttggtgatgc tgctaagaac 180
ttagctgctt ctaacccaaa aaacaccatc tttgatatta agagattgat cggtatgaag 240
tatgatgccc cagaggtcca aagagacttg aagcgtcttc cttacactgt caagagcaag 300
aacggccaac ctgtcgtttc tgtcgagtac aagggtgagg agaagtcttt cactcctgag 360
gagatttccg ccatggtctt gggtaagatg aagttgatcg ctgaggacta cttaggaaag 420
aaagtcactc atgctgtcgt taccgttcca gcctacttca acgacgctca acgtcaagcc 480
actaaggatg ccggtctcat cgccggtttg actgttctga gaattgtgaa cgagcctacc 540
gccgctgccc ttgcttacgg tttggacaag actggtgagg aaagacagat catcgtctac 600
gacttgggtg gaggaacctt cgatgtttct ctgctttcta ttgagggtgg tgctttcgag 660
gttcttgcta ccgccggtga cacccacttg ggtggtgagg actttgacta cagagttgtt 720
cgccacttcg ttaagatttt caagaagaag cataacattg acatcagcaa caatgataag 780
gctttaggta agctgaagag agaggtcgaa aaggccaagc gtactttgtc ttcccagatg 840
actaccagaa ttgagattga ctctttcgtc gacggtatcg acttctctga gcaactgtct 900
agagctaagt ttgaggagat caacattgaa ttattcaaga agacactgaa accagttgaa 960
caagtcctca aagacgctgg tgtcaagaaa tctgaaattg atgacattgt cttggttggt 1020
ggttctacca gaattccaaa ggttcaacaa ttattggagg attactttga cggaaagaag 1080
gcttctaagg gaattaaccc agatgaagct gtcgcatacg gtgctgctgt tcaggctggt 1140
gttttgtctg gtgatcaaga tacaggtgac ctggtactgc ttgatgtatg tccccttaca 1200
cttggtattg aaactgtggg aggtgtcatg accaaactga ttccaaggaa cacagtggtg 1260
cctaccaaga agtctcagat cttttctaca gcttctgata atcaaccaac tgttacaatc 1320
aaggtctatg aaggtgaaag acccctgaca aaagacaatc atcttctggg tacatttgat 1380
ctgactggaa ttcctcctgc tcctcgtggg gtcccacaga ttgaagtcac ctttgagata 1440
gatgtgaatg gtattcttcg agtgacagct gaagacaagg gtacagggaa caaaaataag 1500
atcacaatca ccaatgacca gaatcgcctg acacctgaag aaatcgaaag gatggttaat 1560
gatgctgaga agtttgctga ggaagacaaa aagctcaagg agcgcattga tactagaaat 1620
gagttggaaa gctatgccta ttctctaaag aatcagattg gagataaaga aaagctggga 1680
ggtaaacttt cctctgaaga taaggagacc atggaaaaag ctgtagaaga aaagattgaa 1740
tggctggaaa gccaccaaga tgctgacatt gaagacttca aagctaagaa gaaggaactg 1800
gaagaaattg ttcaaccaat tatcagcaaa ctctatggaa gtgcaggccc tcccccaact 1860
ggtgaagagg atacagcaga acatgatgag ttgtag                           1896

<210> 58
<211> 631
<212> PRT
<213> Artificial Sequence

<220> 
<223> chimeric BIP

<400> 58
Asp Asp Val Glu Ser Tyr Gly Thr Val Ile Gly Ile Asp Leu Gly Thr
 1               5                  10                  15      
Thr Tyr Ser Cys Val Gly Val Met Lys Ser Gly Arg Val Glu Ile Leu
            20                  25                  30          
Ala Asn Asp Gln Gly Asn Arg Ile Thr Pro Ser Tyr Val Ser Phe Thr
        35                  40                  45              
Glu Asp Glu Arg Leu Val Gly Asp Ala Ala Lys Asn Leu Ala Ala Ser
    50                  55                  60                  
Asn Pro Lys Asn Thr Ile Phe Asp Ile Lys Arg Leu Ile Gly Met Lys
65                  70                  75                  80  
Tyr Asp Ala Pro Glu Val Gln Arg Asp Leu Lys Arg Leu Pro Tyr Thr
                85                  90                  95      
Val Lys Ser Lys Asn Gly Gln Pro Val Val Ser Val Glu Tyr Lys Gly
            100                 105                 110         
Glu Glu Lys Ser Phe Thr Pro Glu Glu Ile Ser Ala Met Val Leu Gly
        115                 120                 125             
Lys Met Lys Leu Ile Ala Glu Asp Tyr Leu Gly Lys Lys Val Thr His
    130                 135                 140                 
Ala Val Val Thr Val Pro Ala Tyr Phe Asn Asp Ala Gln Arg Gln Ala
145                 150                 155                 160 
Thr Lys Asp Ala Gly Leu Ile Ala Gly Leu Thr Val Leu Arg Ile Val
                165                 170                 175     
Asn Glu Pro Thr Ala Ala Ala Leu Ala Tyr Gly Leu Asp Lys Thr Gly
            180                 185                 190         
Glu Glu Arg Gln Ile Ile Val Tyr Asp Leu Gly Gly Gly Thr Phe Asp
        195                 200                 205             
Val Ser Leu Leu Ser Ile Glu Gly Gly Ala Phe Glu Val Leu Ala Thr
    210                 215                 220                 
Ala Gly Asp Thr His Leu Gly Gly Glu Asp Phe Asp Tyr Arg Val Val
225                 230                 235                 240 
Arg His Phe Val Lys Ile Phe Lys Lys Lys His Asn Ile Asp Ile Ser
                245                 250                 255     
Asn Asn Asp Lys Ala Leu Gly Lys Leu Lys Arg Glu Val Glu Lys Ala
            260                 265                 270         
Lys Arg Thr Leu Ser Ser Gln Met Thr Thr Arg Ile Glu Ile Asp Ser
        275                 280                 285             
Phe Val Asp Gly Ile Asp Phe Ser Glu Gln Leu Ser Arg Ala Lys Phe
    290                 295                 300                 
Glu Glu Ile Asn Ile Glu Leu Phe Lys Lys Thr Leu Lys Pro Val Glu
305                 310                 315                 320 
Gln Val Leu Lys Asp Ala Gly Val Lys Lys Ser Glu Ile Asp Asp Ile
                325                 330                 335     
Val Leu Val Gly Gly Ser Thr Arg Ile Pro Lys Val Gln Gln Leu Leu
            340                 345                 350         
Glu Asp Tyr Phe Asp Gly Lys Lys Ala Ser Lys Gly Ile Asn Pro Asp
        355                 360                 365             
Glu Ala Val Ala Tyr Gly Ala Ala Val Gln Ala Gly Val Leu Ser Gly
    370                 375                 380                 
Asp Gln Asp Thr Gly Asp Leu Val Leu Leu Asp Val Cys Pro Leu Thr
385                 390                 395                 400 
Leu Gly Ile Glu Thr Val Gly Gly Val Met Thr Lys Leu Ile Pro Arg
                405                 410                 415     
Asn Thr Val Val Pro Thr Lys Lys Ser Gln Ile Phe Ser Thr Ala Ser
            420                 425                 430         
Asp Asn Gln Pro Thr Val Thr Ile Lys Val Tyr Glu Gly Glu Arg Pro
        435                 440                 445             
Leu Thr Lys Asp Asn His Leu Leu Gly Thr Phe Asp Leu Thr Gly Ile
    450                 455                 460                 
Pro Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val Thr Phe Glu Ile
465                 470                 475                 480 
Asp Val Asn Gly Ile Leu Arg Val Thr Ala Glu Asp Lys Gly Thr Gly
                485                 490                 495     
Asn Lys Asn Lys Ile Thr Ile Thr Asn Asp Gln Asn Arg Leu Thr Pro
            500                 505                 510         
Glu Glu Ile Glu Arg Met Val Asn Asp Ala Glu Lys Phe Ala Glu Glu
        515                 520                 525             
Asp Lys Lys Leu Lys Glu Arg Ile Asp Thr Arg Asn Glu Leu Glu Ser
    530                 535                 540                 
Tyr Ala Tyr Ser Leu Lys Asn Gln Ile Gly Asp Lys Glu Lys Leu Gly
545                 550                 555                 560 
Gly Lys Leu Ser Ser Glu Asp Lys Glu Thr Met Glu Lys Ala Val Glu
                565                 570                 575     
Glu Lys Ile Glu Trp Leu Glu Ser His Gln Asp Ala Asp Ile Glu Asp
            580                 585                 590         
Phe Lys Ala Lys Lys Lys Glu Leu Glu Glu Ile Val Gln Pro Ile Ile
        595                 600                 605             
Ser Lys Leu Tyr Gly Ser Ala Gly Pro Pro Pro Thr Gly Glu Glu Asp
    610                 615                 620                 
Thr Ala Glu His Asp Glu Leu
625                 630     


<210> 59
<211> 254
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pichia pastoris PDI1 promoter

<400> 59
aacacgaaca ctgtaaatag aataaaagaa aacttggata gtagaacttc aatgtagtgt 60
ttctattgtc ttacgcggct ctttagattg caatccccag aatggaatcg tccatctttc 120
tcaacccact caaagataat ctaccagaca tacctacgcc ctccatccca gcaccacgtc 180
gcgatcaccc ctaaaacttc aataattgaa cacgtactga tttccaaacc ttcttcttct 240
tcctatctat aaga                                                   254

<210> 60
<211> 2775
<212> DNA
<213> Pichia pastoris

<400> 60
atgacagcta atgaaaatcc ttttgagaat gagctgacag gatcttctga atctgccccc 60
cctgcattgg aatcgaagac tggagagtct cttaagtatt gcaaatatac cgtggatcag 120
gtcatagaag agtttcaaac ggatggtctc aaaggattgt gcaattccca ggacatcgta 180
tatcggaggt ctgttcatgg gccaaatgaa atggaagtcg aagaggaaga gagtcttttt 240
tcgaaattct tgtcaagttt ctacagcgat ccattgattc tgttactgat gggttccgct 300
gtgattagct ttttgatgtc taacattgat gatgcgatat ctatcactat ggcaattacg 360
atcgttgtca cagttggatt tgttcaagag tatcgatccg agaaatcatt ggaggcattg 420
aacaagttag tccctgccga agctcatcta actaggaatg ggaacactga aactgttctt 480
gctgccaacc tagtcccagg agacttggtg gatttttcgg ttggtgacag aattccggct 540
gatgtgagaa ttattcacgc ttcccacttg agtatcgacg agagcaacct aactggtgaa 600
aatgaaccag tttctaaaga cagcaaacct gttgaaagtg atgacccaaa cattcccttg 660
aacagccgtt catgtattgg gtatatgggc actttagttc gtgatggtaa tggcaaaggt 720
attgtcatcg gaacagccaa aaacacagct tttggctctg ttttcgaaat gatgagctct 780
attgagaaac caaagactcc tcttcaacag gctatggata aacttggtaa ggatttgtct 840
gctttttcct tcggaatcat cggccttatt tgcttggttg gtgtttttca aggtagaccc 900
tggttggaaa tgttccagat ctctgtatcc ttggctgttg ctgcgattcc agaaggtctt 960
cctattattg tgactgtgac tcttgctctt ggtgtgttgc gtatggctaa acagagggcc 1020
atcgtcaaaa gactgcctag tgttgaaact ttgggatccg tcaatgttat ctgtagtgat 1080
aagacgggaa cattgaccca aaatcatatg accgttaaca gattatggac tgtggatatg 1140
ggcgatgaat tcttgaaaat tgaacaaggg gagtcctatg ccaattatct caaacccgat 1200
acgctaaaag ttctgcaaac tggtaatata gtcaacaatg ccaaatattc aaatgaaaag 1260
gaaaaatacc tcggaaaccc aactgatatt gcaattattg aatctttaga aaaatttgat 1320
ttgcaggaca ttagagcaac aaaggaaaga atgttggaga ttccattttc ttcgtccaag 1380
aaatatcagg ccgtcagtgt tcactctgga gacaaaagca aatctgaaat ttttgttaaa 1440
ggcgctctga acaaagtttt ggaaagatgt tcaagatatt acaatgctga aggtatcgcc 1500
actccactca cagatgaaat tagaagaaaa tccttgcaaa tggccgatac gttagcatct 1560
tcaggattga gaatactgtc gtttgcttac gacaaaggca attttgaaga aactggcgat 1620
ggaccatcgg atatgatctt ttgtggtctt ttaggtatga acgatcctcc tagaccatct 1680
gtaagtaaat caattttgaa attcatgaga ggtggggttc acattattat gattacagga 1740
gattcagaat ccacggccgt agccgttgcc aaacaggtcg gaatggtaat tgacaattca 1800
aaatatgctg tcctcagtgg agacgatata gatgctatga gtacagagca actgtctcag 1860
gcgatctcac attgttctgt atttgcccgg actactccaa aacataaggt gtccattgta 1920
agagcactac aggccagagg agatattgtt gcaatgactg gtgacggtgt caatgatgcc 1980
ccagctctaa aactggccga catcggaatt gccatgggta atatggggac cgatgttgcc 2040
aaagaggcag ccgacatggt tttgactgat gatgactttt ctacaatctt atctgcaatc 2100
caggagggta aaggtatttt ctacaacatc cagaactttt taacgttcca actttctact 2160
tcaattgctg ctctttcgtt aattgctctg agtactgctt tcaacctgcc aaatccattg 2220
aatgccatgc agattttgtg gatcaatatt atcatggatg gacctccagc tcagtctttg 2280
ggtgttgagc cagttgataa agctgtgatg aacaaaccac caagaaagcg aaatgataaa 2340
attctgacag gtaaggtgat tcaaagggta gtacaaagta gttttatcat tgtttgtggt 2400
actctgtacg tatacatgca tgagatcaaa gataatgagg tcacagcaag agacactacg 2460
atgaccttta catgctttgt attctttgac atgttcaacg cattaacgac aagacaccat 2520
tctaaaagta ttgcagaact tggatggaat aatactatgt tcaacttttc cgttgcagct 2580
tctattttgg gtcaactagg agctatttac attccatttt tgcagtctat tttccagact 2640
gaacctctga gcctcaaaga tttggtccat ttattgttgt tatcgagttc agtatggatt 2700
gtagacgagc ttcgaaaact ctacgtcagg agacgtgacg catccccata caatggatac 2760
agcatggctg tttga                                                  2775

<210> 61
<211> 924
<212> PRT
<213> Pichia pastoris

<400> 61
Met Thr Ala Asn Glu Asn Pro Phe Glu Asn Glu Leu Thr Gly Ser Ser
 1               5                  10                  15      
Glu Ser Ala Pro Pro Ala Leu Glu Ser Lys Thr Gly Glu Ser Leu Lys
            20                  25                  30          
Tyr Cys Lys Tyr Thr Val Asp Gln Val Ile Glu Glu Phe Gln Thr Asp
        35                  40                  45              
Gly Leu Lys Gly Leu Cys Asn Ser Gln Asp Ile Val Tyr Arg Arg Ser
    50                  55                  60                  
Val His Gly Pro Asn Glu Met Glu Val Glu Glu Glu Glu Ser Leu Phe
65                  70                  75                  80  
Ser Lys Phe Leu Ser Ser Phe Tyr Ser Asp Pro Leu Ile Leu Leu Leu
                85                  90                  95      
Met Gly Ser Ala Val Ile Ser Phe Leu Met Ser Asn Ile Asp Asp Ala
            100                 105                 110         
Ile Ser Ile Thr Met Ala Ile Thr Ile Val Val Thr Val Gly Phe Val
        115                 120                 125             
Gln Glu Tyr Arg Ser Glu Lys Ser Leu Glu Ala Leu Asn Lys Leu Val
    130                 135                 140                 
Pro Ala Glu Ala His Leu Thr Arg Asn Gly Asn Thr Glu Thr Val Leu
145                 150                 155                 160 
Ala Ala Asn Leu Val Pro Gly Asp Leu Val Asp Phe Ser Val Gly Asp
                165                 170                 175     
Arg Ile Pro Ala Asp Val Arg Ile Ile His Ala Ser His Leu Ser Ile
            180                 185                 190         
Asp Glu Ser Asn Leu Thr Gly Glu Asn Glu Pro Val Ser Lys Asp Ser
        195                 200                 205             
Lys Pro Val Glu Ser Asp Asp Pro Asn Ile Pro Leu Asn Ser Arg Ser
    210                 215                 220                 
Cys Ile Gly Tyr Met Gly Thr Leu Val Arg Asp Gly Asn Gly Lys Gly
225                 230                 235                 240 
Ile Val Ile Gly Thr Ala Lys Asn Thr Ala Phe Gly Ser Val Phe Glu
                245                 250                 255     
Met Met Ser Ser Ile Glu Lys Pro Lys Thr Pro Leu Gln Gln Ala Met
            260                 265                 270         
Asp Lys Leu Gly Lys Asp Leu Ser Ala Phe Ser Phe Gly Ile Ile Gly
        275                 280                 285             
Leu Ile Cys Leu Val Gly Val Phe Gln Gly Arg Pro Trp Leu Glu Met
    290                 295                 300                 
Phe Gln Ile Ser Val Ser Leu Ala Val Ala Ala Ile Pro Glu Gly Leu
305                 310                 315                 320 
Pro Ile Ile Val Thr Val Thr Leu Ala Leu Gly Val Leu Arg Met Ala
                325                 330                 335     
Lys Gln Arg Ala Ile Val Lys Arg Leu Pro Ser Val Glu Thr Leu Gly
            340                 345                 350         
Ser Val Asn Val Ile Cys Ser Asp Lys Thr Gly Thr Leu Thr Gln Asn
        355                 360                 365             
His Met Thr Val Asn Arg Leu Trp Thr Val Asp Met Gly Asp Glu Phe
    370                 375                 380                 
Leu Lys Ile Glu Gln Gly Glu Ser Tyr Ala Asn Tyr Leu Lys Pro Asp
385                 390                 395                 400 
Thr Leu Lys Val Leu Gln Thr Gly Asn Ile Val Asn Asn Ala Lys Tyr
                405                 410                 415     
Ser Asn Glu Lys Glu Lys Tyr Leu Gly Asn Pro Thr Asp Ile Ala Ile
            420                 425                 430         
Ile Glu Ser Leu Glu Lys Phe Asp Leu Gln Asp Ile Arg Ala Thr Lys
        435                 440                 445             
Glu Arg Met Leu Glu Ile Pro Phe Ser Ser Ser Lys Lys Tyr Gln Ala
    450                 455                 460                 
Val Ser Val His Ser Gly Asp Lys Ser Lys Ser Glu Ile Phe Val Lys
465                 470                 475                 480 
Gly Ala Leu Asn Lys Val Leu Glu Arg Cys Ser Arg Tyr Tyr Asn Ala
                485                 490                 495     
Glu Gly Ile Ala Thr Pro Leu Thr Asp Glu Ile Arg Arg Lys Ser Leu
            500                 505                 510         
Gln Met Ala Asp Thr Leu Ala Ser Ser Gly Leu Arg Ile Leu Ser Phe
        515                 520                 525             
Ala Tyr Asp Lys Gly Asn Phe Glu Glu Thr Gly Asp Gly Pro Ser Asp
    530                 535                 540                 
Met Ile Phe Cys Gly Leu Leu Gly Met Asn Asp Pro Pro Arg Pro Ser
545                 550                 555                 560 
Val Ser Lys Ser Ile Leu Lys Phe Met Arg Gly Gly Val His Ile Ile
                565                 570                 575     
Met Ile Thr Gly Asp Ser Glu Ser Thr Ala Val Ala Val Ala Lys Gln
            580                 585                 590         
Val Gly Met Val Ile Asp Asn Ser Lys Tyr Ala Val Leu Ser Gly Asp
        595                 600                 605             
Asp Ile Asp Ala Met Ser Thr Glu Gln Leu Ser Gln Ala Ile Ser His
    610                 615                 620                 
Cys Ser Val Phe Ala Arg Thr Thr Pro Lys His Lys Val Ser Ile Val
625                 630                 635                 640 
Arg Ala Leu Gln Ala Arg Gly Asp Ile Val Ala Met Thr Gly Asp Gly
                645                 650                 655     
Val Asn Asp Ala Pro Ala Leu Lys Leu Ala Asp Ile Gly Ile Ala Met
            660                 665                 670         
Gly Asn Met Gly Thr Asp Val Ala Lys Glu Ala Ala Asp Met Val Leu
        675                 680                 685             
Thr Asp Asp Asp Phe Ser Thr Ile Leu Ser Ala Ile Gln Glu Gly Lys
    690                 695                 700                 
Gly Ile Phe Tyr Asn Ile Gln Asn Phe Leu Thr Phe Gln Leu Ser Thr
705                 710                 715                 720 
Ser Ile Ala Ala Leu Ser Leu Ile Ala Leu Ser Thr Ala Phe Asn Leu
                725                 730                 735     
Pro Asn Pro Leu Asn Ala Met Gln Ile Leu Trp Ile Asn Ile Ile Met
            740                 745                 750         
Asp Gly Pro Pro Ala Gln Ser Leu Gly Val Glu Pro Val Asp Lys Ala
        755                 760                 765             
Val Met Asn Lys Pro Pro Arg Lys Arg Asn Asp Lys Ile Leu Thr Gly
    770                 775                 780                 
Lys Val Ile Gln Arg Val Val Gln Ser Ser Phe Ile Ile Val Cys Gly
785                 790                 795                 800 
Thr Leu Tyr Val Tyr Met His Glu Ile Lys Asp Asn Glu Val Thr Ala
                805                 810                 815     
Arg Asp Thr Thr Met Thr Phe Thr Cys Phe Val Phe Phe Asp Met Phe
            820                 825                 830         
Asn Ala Leu Thr Thr Arg His His Ser Lys Ser Ile Ala Glu Leu Gly
        835                 840                 845             
Trp Asn Asn Thr Met Phe Asn Phe Ser Val Ala Ala Ser Ile Leu Gly
    850                 855                 860                 
Gln Leu Gly Ala Ile Tyr Ile Pro Phe Leu Gln Ser Ile Phe Gln Thr
865                 870                 875                 880 
Glu Pro Leu Ser Leu Lys Asp Leu Val His Leu Leu Leu Leu Ser Ser
                885                 890                 895     
Ser Val Trp Ile Val Asp Glu Leu Arg Lys Leu Tyr Val Arg Arg Arg
            900                 905                 910         
Asp Ala Ser Pro Tyr Asn Gly Tyr Ser Met Ala Val
        915                 920                 


<210> 62
<211> 3186
<212> DNA
<213> Artificial Sequence

<220> 
<223> Arabidopsis thalian DNA encoding ECA1
      codon-optimized for Pichia expression

<400> 62
atgggaaagg gttccgagga cctggttaag aaagaatccc tgaactccac tccagttaac 60
tctgacactt tcccagcttg ggctaaggat gttgctgagt gcgaagagca cttcgttgtt 120
tccagagaga agggtttgtc ctccgacgaa gtcttgaaga gacaccaaat ctacggactg 180
aacgagttgg aaaagccaga gggaacctcc atcttcaagc tgatcttgga gcagttcaac 240
gacacccttg tcagaatttt gttggctgcc gctgttattt ccttcgtcct ggcttttttt 300
gatggtgacg agggtggtga aatgggtatc actgccttcg ttgagccttt ggtcatcttc 360
ctgatcttga tcgttaacgc catcgttggt atctggcaag agactaacgc tgaaaaggct 420
ttggaggcct tgaaagagat tcaatcccag caggctaccg ttatgagaga tggtactaag 480
gtttcctcct tgccagctaa agaattggtt ccaggtgaca tcgttgagct gagagttggt 540
gataaggttc cagccgacat gagagttgtt gctttgatct cctccacctt gagagttgaa 600
caaggttccc tgactggtga atctgaggct gtttccaaga ctactaagca cgttgacgag 660
aacgctgaca tccagggtaa aaagtgcatg gttttcgccg gtactaccgt tgttaacggt 720
aactgcatct gtttggtcac tgacactgga atgaacaccg agatcggtag agttcactcc 780
caaatccaag aagctgctca acacgaagag gacaccccat tgaagaagaa gctgaacgag 840
ttcggagagg tcttgaccat gatcatcgga ttgatctgtg ccctggtctg gttgatcaac 900
gtcaagtact tcttgtcctg ggaatacgtt gatggatggc caagaaactt caagttctcc 960
ttcgagaagt gcacctacta cttcgagatc gctgttgctt tggctgttgc tgctattcca 1020
gagggattgc cagctgttat caccacttgc ttggccttgg gtactagaaa gatggctcag 1080
aagaacgccc ttgttagaaa gttgccatcc gttgagactt tgggttgtac taccgtcatc 1140
tgttccgaca agactggtac tttgactacc aaccagatgg ccgtttccaa attggttgcc 1200
atgggttcca gaatcggtac tctgagatcc ttcaacgtcg agggaacttc ttttgaccca 1260
agagatggaa agattgagga ctggccaatg ggtagaatgg acgccaactt gcagatgatt 1320
gctaagatcg ccgctatctg taacgacgct aacgttgagc aatccgacca acagttcgtt 1380
tccagaggaa tgccaactga ggctgccttg aaggttttgg tcgagaagat gggtttccca 1440
gaaggattga acgaggcttc ttccgatggt gacgtcttga gatgttgcag actgtggagt 1500
gagttggagc agagaatcgc tactttggag ttcgacagag atagaaagtc catgggtgtc 1560
atggttgatt cttcctccgg taacaagttg ttgttggtca aaggagcagt tgaaaacgtt 1620
ttggagagat ccacccacat tcaattgctg gacggttcca agagagaatt ggaccagtac 1680
tccagagact tgatcttgca gtccttgaga gacatgtcct tgtccgcctt gagatgtttg 1740
ggtttcgctt actctgacgt tccatccgat ttcgctactt acgatggttc tgaggatcat 1800
ccagctcacc aacagttgct gaacccatcc aactactcct ccatcgaatc caacctgatc 1860
ttcgttggtt tcgtcggtct tagagaccca ccaagaaaag aagttagaca ggccatcgct 1920
gattgtagaa ccgccggtat cagagttatg gtcatcaccg gagataacaa gtccactgcc 1980
gaggctattt gtagagagat cggagttttc gaggctgacg aggacatttc ttccagatcc 2040
ctgaccggta ttgagttcat ggacgtccaa gaccagaaga accacttgag acagaccggt 2100
ggtttgttgt tctccagagc cgaaccaaag cacaagcaag agattgtcag actgctgaaa 2160
gaggacggag aagttgttgc tatgaccggt gatggtgtta atgacgcccc agctttgaag 2220
ttggctgaca tcggtgttgc tatgggaatt tccggtactg aagttgctaa ggaagcctcc 2280
gatatggttt tggctgacga caacttttca actatcgttg ctgctgtcgg agaaggtaga 2340
agtatctaca acaacatgaa agcctttatc agatacatga tttcctccaa catcggtgaa 2400
gttgcctcca ttttcttgac tgctgccttg ggtattcctg agggaatgat cccagttcag 2460
ttgttgtggg ttaacttggt tactgacggt ccacctgcta ctgctttggg tttcaaccca 2520
ccagacaaag acattatgaa gaagccacca agaagatccg acgattcctt gatcaccgcc 2580
tggatcttgt tcagatacat ggtcatcggt ctttatgttg gtgttgccac cgtcggtgtt 2640
ttcatcatct ggtacaccca ctcttccttc atgggtattg acttgtctca agatggtcat 2700
tctttggttt cctactccca attggctcat tggggacaat gttcttcctg ggagggtttc 2760
aaggtttccc cattcactgc tggttcccag actttctcct tcgattccaa cccatgtgac 2820
tacttccagc agggaaagat caaggcttcc accttgtctt tgtccgtttt ggtcgccatt 2880
gagatgttca actccctgaa cgctttgtct gaggacggtt ccttggttac tatgccacct 2940
tgggtgaacc catggttgtt gttggctatg gctgtttcct tcggattgca cttcgtcatc 3000
ctgtacgttc cattcttggc ccaggttttc ggtattgttc cactgtcctt gaacgagtgg 3060
ttgttggtct tggccgtttc tttgccagtt atcctgatcg acgaggtttt gaagttcgtt 3120
ggtagatgca cctctggtta cagatactcc ccaagaactc tgtccaccaa gcagaaagaa 3180
gagtaa                                                            3186

<210> 63
<211> 1061
<212> PRT
<213> Arabidopsis thaliana

<400> 63
Met Gly Lys Gly Ser Glu Asp Leu Val Lys Lys Glu Ser Leu Asn Ser
 1               5                  10                  15      
Thr Pro Val Asn Ser Asp Thr Phe Pro Ala Trp Ala Lys Asp Val Ala
            20                  25                  30          
Glu Cys Glu Glu His Phe Val Val Ser Arg Glu Lys Gly Leu Ser Ser
        35                  40                  45              
Asp Glu Val Leu Lys Arg His Gln Ile Tyr Gly Leu Asn Glu Leu Glu
    50                  55                  60                  
Lys Pro Glu Gly Thr Ser Ile Phe Lys Leu Ile Leu Glu Gln Phe Asn
65                  70                  75                  80  
Asp Thr Leu Val Arg Ile Leu Leu Ala Ala Ala Val Ile Ser Phe Val
                85                  90                  95      
Leu Ala Phe Phe Asp Gly Asp Glu Gly Gly Glu Met Gly Ile Thr Ala
            100                 105                 110         
Phe Val Glu Pro Leu Val Ile Phe Leu Ile Leu Ile Val Asn Ala Ile
        115                 120                 125             
Val Gly Ile Trp Gln Glu Thr Asn Ala Glu Lys Ala Leu Glu Ala Leu
    130                 135                 140                 
Lys Glu Ile Gln Ser Gln Gln Ala Thr Val Met Arg Asp Gly Thr Lys
145                 150                 155                 160 
Val Ser Ser Leu Pro Ala Lys Glu Leu Val Pro Gly Asp Ile Val Glu
                165                 170                 175     
Leu Arg Val Gly Asp Lys Val Pro Ala Asp Met Arg Val Val Ala Leu
            180                 185                 190         
Ile Ser Ser Thr Leu Arg Val Glu Gln Gly Ser Leu Thr Gly Glu Ser
        195                 200                 205             
Glu Ala Val Ser Lys Thr Thr Lys His Val Asp Glu Asn Ala Asp Ile
    210                 215                 220                 
Gln Gly Lys Lys Cys Met Val Phe Ala Gly Thr Thr Val Val Asn Gly
225                 230                 235                 240 
Asn Cys Ile Cys Leu Val Thr Asp Thr Gly Met Asn Thr Glu Ile Gly
                245                 250                 255     
Arg Val His Ser Gln Ile Gln Glu Ala Ala Gln His Glu Glu Asp Thr
            260                 265                 270         
Pro Leu Lys Lys Lys Leu Asn Glu Phe Gly Glu Val Leu Thr Met Ile
        275                 280                 285             
Ile Gly Leu Ile Cys Ala Leu Val Trp Leu Ile Asn Val Lys Tyr Phe
    290                 295                 300                 
Leu Ser Trp Glu Tyr Val Asp Gly Trp Pro Arg Asn Phe Lys Phe Ser
305                 310                 315                 320 
Phe Glu Lys Cys Thr Tyr Tyr Phe Glu Ile Ala Val Ala Leu Ala Val
                325                 330                 335     
Ala Ala Ile Pro Glu Gly Leu Pro Ala Val Ile Thr Thr Cys Leu Ala
            340                 345                 350         
Leu Gly Thr Arg Lys Met Ala Gln Lys Asn Ala Leu Val Arg Lys Leu
        355                 360                 365             
Pro Ser Val Glu Thr Leu Gly Cys Thr Thr Val Ile Cys Ser Asp Lys
    370                 375                 380                 
Thr Gly Thr Leu Thr Thr Asn Gln Met Ala Val Ser Lys Leu Val Ala
385                 390                 395                 400 
Met Gly Ser Arg Ile Gly Thr Leu Arg Ser Phe Asn Val Glu Gly Thr
                405                 410                 415     
Ser Phe Asp Pro Arg Asp Gly Lys Ile Glu Asp Trp Pro Met Gly Arg
            420                 425                 430         
Met Asp Ala Asn Leu Gln Met Ile Ala Lys Ile Ala Ala Ile Cys Asn
        435                 440                 445             
Asp Ala Asn Val Glu Gln Ser Asp Gln Gln Phe Val Ser Arg Gly Met
    450                 455                 460                 
Pro Thr Glu Ala Ala Leu Lys Val Leu Val Glu Lys Met Gly Phe Pro
465                 470                 475                 480 
Glu Gly Leu Asn Glu Ala Ser Ser Asp Gly Asp Val Leu Arg Cys Cys
                485                 490                 495     
Arg Leu Trp Ser Glu Leu Glu Gln Arg Ile Ala Thr Leu Glu Phe Asp
            500                 505                 510         
Arg Asp Arg Lys Ser Met Gly Val Met Val Asp Ser Ser Ser Gly Asn
        515                 520                 525             
Lys Leu Leu Leu Val Lys Gly Ala Val Glu Asn Val Leu Glu Arg Ser
    530                 535                 540                 
Thr His Ile Gln Leu Leu Asp Gly Ser Lys Arg Glu Leu Asp Gln Tyr
545                 550                 555                 560 
Ser Arg Asp Leu Ile Leu Gln Ser Leu Arg Asp Met Ser Leu Ser Ala
                565                 570                 575     
Leu Arg Cys Leu Gly Phe Ala Tyr Ser Asp Val Pro Ser Asp Phe Ala
            580                 585                 590         
Thr Tyr Asp Gly Ser Glu Asp His Pro Ala His Gln Gln Leu Leu Asn
        595                 600                 605             
Pro Ser Asn Tyr Ser Ser Ile Glu Ser Asn Leu Ile Phe Val Gly Phe
    610                 615                 620                 
Val Gly Leu Arg Asp Pro Pro Arg Lys Glu Val Arg Gln Ala Ile Ala
625                 630                 635                 640 
Asp Cys Arg Thr Ala Gly Ile Arg Val Met Val Ile Thr Gly Asp Asn
                645                 650                 655     
Lys Ser Thr Ala Glu Ala Ile Cys Arg Glu Ile Gly Val Phe Glu Ala
            660                 665                 670         
Asp Glu Asp Ile Ser Ser Arg Ser Leu Thr Gly Ile Glu Phe Met Asp
        675                 680                 685             
Val Gln Asp Gln Lys Asn His Leu Arg Gln Thr Gly Gly Leu Leu Phe
    690                 695                 700                 
Ser Arg Ala Glu Pro Lys His Lys Gln Glu Ile Val Arg Leu Leu Lys
705                 710                 715                 720 
Glu Asp Gly Glu Val Val Ala Met Thr Gly Asp Gly Val Asn Asp Ala
                725                 730                 735     
Pro Ala Leu Lys Leu Ala Asp Ile Gly Val Ala Met Gly Ile Ser Gly
            740                 745                 750         
Thr Glu Val Ala Lys Glu Ala Ser Asp Met Val Leu Ala Asp Asp Asn
        755                 760                 765             
Phe Ser Thr Ile Val Ala Ala Val Gly Glu Gly Arg Ser Ile Tyr Asn
    770                 775                 780                 
Asn Met Lys Ala Phe Ile Arg Tyr Met Ile Ser Ser Asn Ile Gly Glu
785                 790                 795                 800 
Val Ala Ser Ile Phe Leu Thr Ala Ala Leu Gly Ile Pro Glu Gly Met
                805                 810                 815     
Ile Pro Val Gln Leu Leu Trp Val Asn Leu Val Thr Asp Gly Pro Pro
            820                 825                 830         
Ala Thr Ala Leu Gly Phe Asn Pro Pro Asp Lys Asp Ile Met Lys Lys
        835                 840                 845             
Pro Pro Arg Arg Ser Asp Asp Ser Leu Ile Thr Ala Trp Ile Leu Phe
    850                 855                 860                 
Arg Tyr Met Val Ile Gly Leu Tyr Val Gly Val Ala Thr Val Gly Val
865                 870                 875                 880 
Phe Ile Ile Trp Tyr Thr His Ser Ser Phe Met Gly Ile Asp Leu Ser
                885                 890                 895     
Gln Asp Gly His Ser Leu Val Ser Tyr Ser Gln Leu Ala His Trp Gly
            900                 905                 910         
Gln Cys Ser Ser Trp Glu Gly Phe Lys Val Ser Pro Phe Thr Ala Gly
        915                 920                 925             
Ser Gln Thr Phe Ser Phe Asp Ser Asn Pro Cys Asp Tyr Phe Gln Gln
    930                 935                 940                 
Gly Lys Ile Lys Ala Ser Thr Leu Ser Leu Ser Val Leu Val Ala Ile
945                 950                 955                 960 
Glu Met Phe Asn Ser Leu Asn Ala Leu Ser Glu Asp Gly Ser Leu Val
                965                 970                 975     
Thr Met Pro Pro Trp Val Asn Pro Trp Leu Leu Leu Ala Met Ala Val
            980                 985                 990         
Ser Phe Gly Leu His Phe Val Ile Leu Tyr Val Pro Phe Leu Ala Gln
        995                 1000                1005            
Val Phe Gly Ile Val Pro Leu Ser Leu Asn Glu Trp Leu Leu Val Leu
    1010                1015                1020                
Ala Val Ser Leu Pro Val Ile Leu Ile Asp Glu Val Leu Lys Phe Val
1025                1030                1035               1040 
Gly Arg Cys Thr Ser Gly Tyr Arg Tyr Ser Pro Arg Thr Leu Ser Thr
                1045                1050                1055    
Lys Gln Lys Glu Glu
            1060    


<210> 64
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> PpPMR1/UP PCR primer

<400> 64
gaattcatga cagctaatga aaatcctttt gagaatgag                        39

<210> 65
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> PpPMR1/LP PCR primer

<400> 65
ggccggcctc aaacagccat gctgtatcca ttgtatg                          37

<210> 66
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> 5'AOX1 PCR primer

<400> 66
gcgactggtt ccaattgaca agctt                                       25

<210> 67
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> PpPMR1/cLP PCR primer

<400> 67
ggttgctctc gtcgatactc aagtgggaag                                  30

<210> 68
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> AtECA1/cLP PCR primer

<400> 68
gtcggctgga accttatcac caactctcag                                  30

<210> 69
<211> 1314
<212> DNA
<213> Homo sapiens

<400> 69
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagcttac 60
ccatacgacg tcccagacta cgcttaccca tacgacgtcc cagactacgc tgagcccgcc 120
gtctacttca aggagcagtt tctggacgga gacgggtgga cttcccgctg gatcgaatcc 180
aaacacaagt cagattttgg caaattcgtt ctcagttccg gcaagttcta cggtgacgag 240
gagaaagata aaggtttgca gacaagccag gatgcacgct tttatgctct gtcggccagt 300
ttcgagcctt tcagcaacaa aggccagacg ctggtggtgc agttcacggt gaaacatgag 360
cagaacatcg actgtggggg cggctatgtg aagctgtttc ctaatagttt ggaccagaca 420
gacatgcacg gagactcaga atacaacatc atgtttggtc ccgacatctg tggccctggc 480
accaagaagg ttcatgtcat cttcaactac aagggcaaga acgtgctgat caacaaggac 540
atccgttgca aggatgatga gtttacacac ctgtacacac tgattgtgcg gccagacaac 600
acctatgagg tgaagattga caacagccag gtggagtccg gctccttgga agacgattgg 660
gacttcctgc cacccaagaa gataaaggat cctgatgctt caaaaccgga agactgggat 720
gagcgggcca agatcgatga tcccacagac tccaagcctg aggactggga caagcccgag 780
catatccctg accctgatgc taagaagccc gaggactggg atgaagagat ggacggagag 840
tgggaacccc cagtgattca gaaccctgag tacaagggtg agtggaagcc ccggcagatc 900
gacaacccag attacaaggg cacttggatc cacccagaaa ttgacaaccc cgagtattct 960
cccgatccca gtatctatgc ctatgataac tttggcgtgc tgggcctgga cctctggcag 1020
gtcaagtctg gcaccatctt tgacaacttc ctcatcacca acgatgaggc atacgctgag 1080
gagtttggca acgagacgtg gggcgtaaca aaggcagcag agaaacaaat gaaggacaaa 1140
caggacgagg agcagaggct taaggaggag gaagaagaca agaaacgcaa agaggaggag 1200
gaggcagagg acaaggagga tgatgaggac aaagatgagg atgaggagga tgaggaggac 1260
aaggaggaag atgaggagga agatgtcccc ggccaggccc atgacgagct gtag       1314

<210> 70
<211> 437
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human calreticulin (hCRT)-protein with
      Saccharomyces cerevisiae mating factor pre-signal
      peptide leader

<400> 70
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
 1               5                  10                  15      
Ala Leu Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Tyr Pro Tyr Asp
            20                  25                  30          
Val Pro Asp Tyr Ala Glu Pro Ala Val Tyr Phe Lys Glu Gln Phe Leu
        35                  40                  45              
Asp Gly Asp Gly Trp Thr Ser Arg Trp Ile Glu Ser Lys His Lys Ser
    50                  55                  60                  
Asp Phe Gly Lys Phe Val Leu Ser Ser Gly Lys Phe Tyr Gly Asp Glu
65                  70                  75                  80  
Glu Lys Asp Lys Gly Leu Gln Thr Ser Gln Asp Ala Arg Phe Tyr Ala
                85                  90                  95      
Leu Ser Ala Ser Phe Glu Pro Phe Ser Asn Lys Gly Gln Thr Leu Val
            100                 105                 110         
Val Gln Phe Thr Val Lys His Glu Gln Asn Ile Asp Cys Gly Gly Gly
        115                 120                 125             
Tyr Val Lys Leu Phe Pro Asn Ser Leu Asp Gln Thr Asp Met His Gly
    130                 135                 140                 
Asp Ser Glu Tyr Asn Ile Met Phe Gly Pro Asp Ile Cys Gly Pro Gly
145                 150                 155                 160 
Thr Lys Lys Val His Val Ile Phe Asn Tyr Lys Gly Lys Asn Val Leu
                165                 170                 175     
Ile Asn Lys Asp Ile Arg Cys Lys Asp Asp Glu Phe Thr His Leu Tyr
            180                 185                 190         
Thr Leu Ile Val Arg Pro Asp Asn Thr Tyr Glu Val Lys Ile Asp Asn
        195                 200                 205             
Ser Gln Val Glu Ser Gly Ser Leu Glu Asp Asp Trp Asp Phe Leu Pro
    210                 215                 220                 
Pro Lys Lys Ile Lys Asp Pro Asp Ala Ser Lys Pro Glu Asp Trp Asp
225                 230                 235                 240 
Glu Arg Ala Lys Ile Asp Asp Pro Thr Asp Ser Lys Pro Glu Asp Trp
                245                 250                 255     
Asp Lys Pro Glu His Ile Pro Asp Pro Asp Ala Lys Lys Pro Glu Asp
            260                 265                 270         
Trp Asp Glu Glu Met Asp Gly Glu Trp Glu Pro Pro Val Ile Gln Asn
        275                 280                 285             
Pro Glu Tyr Lys Gly Glu Trp Lys Pro Arg Gln Ile Asp Asn Pro Asp
    290                 295                 300                 
Tyr Lys Gly Thr Trp Ile His Pro Glu Ile Asp Asn Pro Glu Tyr Ser
305                 310                 315                 320 
Pro Asp Pro Ser Ile Tyr Ala Tyr Asp Asn Phe Gly Val Leu Gly Leu
                325                 330                 335     
Asp Leu Trp Gln Val Lys Ser Gly Thr Ile Phe Asp Asn Phe Leu Ile
            340                 345                 350         
Thr Asn Asp Glu Ala Tyr Ala Glu Glu Phe Gly Asn Glu Thr Trp Gly
        355                 360                 365             
Val Thr Lys Ala Ala Glu Lys Gln Met Lys Asp Lys Gln Asp Glu Glu
    370                 375                 380                 
Gln Arg Leu Lys Glu Glu Glu Glu Asp Lys Lys Arg Lys Glu Glu Glu
385                 390                 395                 400 
Glu Ala Glu Asp Lys Glu Asp Asp Glu Asp Lys Asp Glu Asp Glu Glu
                405                 410                 415     
Asp Glu Glu Asp Lys Glu Glu Asp Glu Glu Glu Asp Val Pro Gly Gln
            420                 425                 430         
Ala His Asp Glu Leu
        435         


<210> 71
<211> 1512
<212> DNA
<213> Homo sapiens

<400> 71
atgcaattca actggaacat caagactgtt gcttccatct tgtccgcttt gactttggct 60
caagcttctg acgttttgga gttgactgac gacaacttcg agtccagaat ttctgacact 120
ggttccgctg gattgatgtt ggttgagttc ttcgctccat ggtgtggtca ttgtaagaga 180
ttggctccag aatacgaagc tgctgctact agattgaagg gtatcgttcc attggctaag 240
gttgactgta ctgctaacac taacacttgt aacaagtacg gtgtttccgg ttacccaact 300
ttgaagatct tcagagatgg tgaagaagct ggagcttacg acggtccaag aactgctgac 360
ggtatcgttt cccacttgaa gaagcaagct ggtccagctt ctgttccatt gagaactgag 420
gaggagttca agaagttcat ctccgacaag gacgcttcta tcgttggttt cttcgacgat 480
tctttctctg aagctcactc cgaattcttg aaggctgctt ccaacttgag agacaactac 540
agattcgctc acactaacgt tgagtccttg gttaacgagt acgacgataa cggtgaaggt 600
atcatcttgt tcagaccatc ccacttgact aacaagttcg aggacaagac agttgcttac 660
actgagcaga agatgacttc cggaaagatc aagaagttta tccaagagaa catcttcggt 720
atctgtccac acatgactga ggacaacaag gacttgattc agggaaagga cttgttgatc 780
gcttactacg acgttgacta cgagaagaac gctaagggtt ccaactactg gagaaacaga 840
gttatgatgg ttgctaagaa gttcttggac gctggtcaca agttgaactt cgctgttgct 900
tctagaaaga ctttctccca cgagttgtct gatttcggat tggaatccac tgctggagag 960
attccagttg ttgctatcag aactgctaag ggagagaagt tcgttatgca agaggagttc 1020
tccagagatg gaaaggcttt ggagagattc ttgcaggatt acttcgacgg taacttgaag 1080
agatacttga agtccgagcc aattccagaa tctaacgacg gtccagttaa agttgttgtt 1140
gctgagaact tcgacgagat cgttaacaac gagaacaagg acgttttgat cgagttttac 1200
gctccttggt gtggacactg taaaaacttg gagccaaagt acaaggaatt gggtgaaaag 1260
ttgtccaagg acccaaacat cgttatcgct aagatggacg ctactgctaa cgatgttcca 1320
tccccatacg aagttagagg tttcccaact atctacttct ccccagctaa caagaagttg 1380
aacccaaaga agtacgaggg aggtagagaa ttgtccgact tcatctccta cttgcagaga 1440
gaggctacta atccaccagt tatccaagag gagaagccaa agaagaagaa gaaagctcac 1500
gacgagttgt ag                                                     1512

<210> 72
<211> 503
<212> PRT
<213> Homo sapiens

<400> 72
Met Gln Phe Asn Trp Asn Ile Lys Thr Val Ala Ser Ile Leu Ser Ala
 1               5                  10                  15      
Leu Thr Leu Ala Gln Ala Ser Asp Val Leu Glu Leu Thr Asp Asp Asn
            20                  25                  30          
Phe Glu Ser Arg Ile Ser Asp Thr Gly Ser Ala Gly Leu Met Leu Val
        35                  40                  45              
Glu Phe Phe Ala Pro Trp Cys Gly His Cys Lys Arg Leu Ala Pro Glu
    50                  55                  60                  
Tyr Glu Ala Ala Ala Thr Arg Leu Lys Gly Ile Val Pro Leu Ala Lys
65                  70                  75                  80  
Val Asp Cys Thr Ala Asn Thr Asn Thr Cys Asn Lys Tyr Gly Val Ser
                85                  90                  95      
Gly Tyr Pro Thr Leu Lys Ile Phe Arg Asp Gly Glu Glu Ala Gly Ala
            100                 105                 110         
Tyr Asp Gly Pro Arg Thr Ala Asp Gly Ile Val Ser His Leu Lys Lys
        115                 120                 125             
Gln Ala Gly Pro Ala Ser Val Pro Leu Arg Thr Glu Glu Glu Phe Lys
    130                 135                 140                 
Lys Phe Ile Ser Asp Lys Asp Ala Ser Ile Val Gly Phe Phe Asp Asp
145                 150                 155                 160 
Ser Phe Ser Glu Ala His Ser Glu Phe Leu Lys Ala Ala Ser Asn Leu
                165                 170                 175     
Arg Asp Asn Tyr Arg Phe Ala His Thr Asn Val Glu Ser Leu Val Asn
            180                 185                 190         
Glu Tyr Asp Asp Asn Gly Glu Gly Ile Ile Leu Phe Arg Pro Ser His
        195                 200                 205             
Leu Thr Asn Lys Phe Glu Asp Lys Thr Val Ala Tyr Thr Glu Gln Lys
    210                 215                 220                 
Met Thr Ser Gly Lys Ile Lys Lys Phe Ile Gln Glu Asn Ile Phe Gly
225                 230                 235                 240 
Ile Cys Pro His Met Thr Glu Asp Asn Lys Asp Leu Ile Gln Gly Lys
                245                 250                 255     
Asp Leu Leu Ile Ala Tyr Tyr Asp Val Asp Tyr Glu Lys Asn Ala Lys
            260                 265                 270         
Gly Ser Asn Tyr Trp Arg Asn Arg Val Met Met Val Ala Lys Lys Phe
        275                 280                 285             
Leu Asp Ala Gly His Lys Leu Asn Phe Ala Val Ala Ser Arg Lys Thr
    290                 295                 300                 
Phe Ser His Glu Leu Ser Asp Phe Gly Leu Glu Ser Thr Ala Gly Glu
305                 310                 315                 320 
Ile Pro Val Val Ala Ile Arg Thr Ala Lys Gly Glu Lys Phe Val Met
                325                 330                 335     
Gln Glu Glu Phe Ser Arg Asp Gly Lys Ala Leu Glu Arg Phe Leu Gln
            340                 345                 350         
Asp Tyr Phe Asp Gly Asn Leu Lys Arg Tyr Leu Lys Ser Glu Pro Ile
        355                 360                 365             
Pro Glu Ser Asn Asp Gly Pro Val Lys Val Val Val Ala Glu Asn Phe
    370                 375                 380                 
Asp Glu Ile Val Asn Asn Glu Asn Lys Asp Val Leu Ile Glu Phe Tyr
385                 390                 395                 400 
Ala Pro Trp Cys Gly His Cys Lys Asn Leu Glu Pro Lys Tyr Lys Glu
                405                 410                 415     
Leu Gly Glu Lys Leu Ser Lys Asp Pro Asn Ile Val Ile Ala Lys Met
            420                 425                 430         
Asp Ala Thr Ala Asn Asp Val Pro Ser Pro Tyr Glu Val Arg Gly Phe
        435                 440                 445             
Pro Thr Ile Tyr Phe Ser Pro Ala Asn Lys Lys Leu Asn Pro Lys Lys
    450                 455                 460                 
Tyr Glu Gly Gly Arg Glu Leu Ser Asp Phe Ile Ser Tyr Leu Gln Arg
465                 470                 475                 480 
Glu Ala Thr Asn Pro Pro Val Ile Gln Glu Glu Lys Pro Lys Lys Lys
                485                 490                 495     
Lys Lys Ala His Asp Glu Leu
            500             


<210> 73
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> hCRT-BstZ17I-HA/UP PCR primer

<400> 73
gtatacccat acgacgtccc agactacgct gagcccgccg tctacttcaa ggagc      55

<210> 74
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> hCRT-PacI/LP PCR primer

<400> 74
ttaattaact acagctcgtc atgggcctgg ccggggacat cttcc                 45

<210> 75
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide that binds CRT

<400> 75
Lys Leu Gly Phe Phe Lys Arg
 1               5          


<210> 76
<211> 1068
<212> DNA
<213> Artificial Sequence

<220> 
<223> Encodes human ERdj3 with Saccharomyces cerevisiae
      mating factor pre-signal peptide leader

<400> 76
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctggt 60
agagacttct acaagatttt gggtgttcca agatccgctt ccatcaagga catcaagaag 120
gcttacagaa agttggcttt gcaattgcac ccagacagaa acccagatga cccacaagct 180
caagagaagt tccaagactt gggtgctgct tacgaagttt tgtccgattc cgagaagaga 240
aagcagtacg acacttacgg tgaagaagga ttgaaggacg gtcaccaatc ttctcacggt 300
gacatcttct cccacttttt cggtgacttc ggtttcatgt tcggtggtac tccaagacaa 360
caggacagaa acatcccaag aggttccgac attatcgttg acttggaggt tacattggaa 420
gaggtttacg ctggtaactt cgttgaagtt gttagaaaca agccagttgc tagacaagct 480
ccaggtaaaa gaaagtgtaa ctgtagacaa gagatgagaa ctactcagtt gggtcctggt 540
agattccaaa tgacacagga agttgtttgc gacgagtgtc caaacgttaa gttggttaac 600
gaagagagaa ctttggaggt tgagatcgag ccaggtgtta gagatggaat ggaataccca 660
ttcatcggtg aaggtgaacc acatgttgat ggtgaacctg gtgacttgag attcagaatc 720
aaagttgtta agcacccaat cttcgagaga agaggtgacg acttgtacac taacgttact 780
atttccttgg ttgaatcctt ggttggtttc gagatggaca tcactcattt ggacggtcac 840
aaggttcaca tttccagaga caagatcact agaccaggtg ctaagttgtg gaagaagggt 900
gaaggattgc caaacttcga caacaacaac atcaagggat ctttgatcat cactttcgac 960
gttgacttcc caaaagagca gttgactgaa gaagctagag agggtatcaa gcagttgttg 1020
aagcaaggtt ccgttcagaa ggtttacaac ggattgcagg gatactaa              1068

<210> 77
<211> 355
<212> PRT
<213> Artificial Sequence

<220> 
<223> Encodes human ERdj3 with Saccharomyces cerevisiae
      mating factor pre-signal peptide leader

<400> 77
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
 1               5                  10                  15      
Ala Leu Ala Gly Arg Asp Phe Tyr Lys Ile Leu Gly Val Pro Arg Ser
            20                  25                  30          
Ala Ser Ile Lys Asp Ile Lys Lys Ala Tyr Arg Lys Leu Ala Leu Gln
        35                  40                  45              
Leu His Pro Asp Arg Asn Pro Asp Asp Pro Gln Ala Gln Glu Lys Phe
    50                  55                  60                  
Gln Asp Leu Gly Ala Ala Tyr Glu Val Leu Ser Asp Ser Glu Lys Arg
65                  70                  75                  80  
Lys Gln Tyr Asp Thr Tyr Gly Glu Glu Gly Leu Lys Asp Gly His Gln
                85                  90                  95      
Ser Ser His Gly Asp Ile Phe Ser His Phe Phe Gly Asp Phe Gly Phe
            100                 105                 110         
Met Phe Gly Gly Thr Pro Arg Gln Gln Asp Arg Asn Ile Pro Arg Gly
        115                 120                 125             
Ser Asp Ile Ile Val Asp Leu Glu Val Thr Leu Glu Glu Val Tyr Ala
    130                 135                 140                 
Gly Asn Phe Val Glu Val Val Arg Asn Lys Pro Val Ala Arg Gln Ala
145                 150                 155                 160 
Pro Gly Lys Arg Lys Cys Asn Cys Arg Gln Glu Met Arg Thr Thr Gln
                165                 170                 175     
Leu Gly Pro Gly Arg Phe Gln Met Thr Gln Glu Val Val Cys Asp Glu
            180                 185                 190         
Cys Pro Asn Val Lys Leu Val Asn Glu Glu Arg Thr Leu Glu Val Glu
        195                 200                 205             
Ile Glu Pro Gly Val Arg Asp Gly Met Glu Tyr Pro Phe Ile Gly Glu
    210                 215                 220                 
Gly Glu Pro His Val Asp Gly Glu Pro Gly Asp Leu Arg Phe Arg Ile
225                 230                 235                 240 
Lys Val Val Lys His Pro Ile Phe Glu Arg Arg Gly Asp Asp Leu Tyr
                245                 250                 255     
Thr Asn Val Thr Ile Ser Leu Val Glu Ser Leu Val Gly Phe Glu Met
            260                 265                 270         
Asp Ile Thr His Leu Asp Gly His Lys Val His Ile Ser Arg Asp Lys
        275                 280                 285             
Ile Thr Arg Pro Gly Ala Lys Leu Trp Lys Lys Gly Glu Gly Leu Pro
    290                 295                 300                 
Asn Phe Asp Asn Asn Asn Ile Lys Gly Ser Leu Ile Ile Thr Phe Asp
305                 310                 315                 320 
Val Asp Phe Pro Lys Glu Gln Leu Thr Glu Glu Ala Arg Glu Gly Ile
                325                 330                 335     
Lys Gln Leu Leu Lys Gln Gly Ser Val Gln Lys Val Tyr Asn Gly Leu
            340                 345                 350         
Gln Gly Tyr
        355 

