                         SEQUENCE LISTING

<110> Zeon Corporation
      Esaki, Motoyuki 
      Sato, Takanori 
      Dorsey, Kristi M.

<120>  Avian vaccines possessing a positive marker gene

<130>  080104

<160>  47    

<170>  PatentIn version 3.1

<210>  1
<211>  1539
<212>  DNA
<213>  Autographa californica nuclear polyhedrosis virus

<220>
<221>  CDS
<222>  (1)..(1539)
<223>  gp64 gene of Autographa californica nuclear polyhedrosis virus


<400>  1
atg gta agc gct att gtt tta tat gtg ctt ttg gcg gcg gcg gcg cat       48
Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala Ala His         
1               5                   10                  15              

tct gcc ttt gcg gcg gag cac tgc aac gcg caa atg aag acg ggt ccg       96
Ser Ala Phe Ala Ala Glu His Cys Asn Ala Gln Met Lys Thr Gly Pro         
            20                  25                  30                  

tac aag att aaa aac ttg gac att acc ccg ccc aag gaa acg ctg caa      144
Tyr Lys Ile Lys Asn Leu Asp Ile Thr Pro Pro Lys Glu Thr Leu Gln         
        35                  40                  45                      

aag gac gtg gaa atc acc atc gtg gag acg gac tac aac gaa aac gtg      192
Lys Asp Val Glu Ile Thr Ile Val Glu Thr Asp Tyr Asn Glu Asn Val         
    50                  55                  60                          

att atc ggc tac aag ggg tac tac cag gcg tat gcg tac aac ggc ggc      240
Ile Ile Gly Tyr Lys Gly Tyr Tyr Gln Ala Tyr Ala Tyr Asn Gly Gly         
65                  70                  75                  80          

tcg ctg gat ccc aac aca cgc gtc gaa gaa acc atg aaa acg ctg aat      288
Ser Leu Asp Pro Asn Thr Arg Val Glu Glu Thr Met Lys Thr Leu Asn         
                85                  90                  95              

gtg ggc aaa gag gat ttg ctt atg tgg agc atc agg cag cag tgc gag      336
Val Gly Lys Glu Asp Leu Leu Met Trp Ser Ile Arg Gln Gln Cys Glu         
            100                 105                 110                 

gtg ggc gaa gag ctg atc gac cgt tgg ggc agt gac agc gac gac tgt      384
Val Gly Glu Glu Leu Ile Asp Arg Trp Gly Ser Asp Ser Asp Asp Cys         
        115                 120                 125                     

ttt cgc gac aac gag ggc cgc ggc cag tgg gtc aaa ggc aaa gag ttg      432
Phe Arg Asp Asn Glu Gly Arg Gly Gln Trp Val Lys Gly Lys Glu Leu         
    130                 135                 140                         

gtg aag cgg cag aat aac aat cac ttt gcg cac cac acg tgc aac aaa      480
Val Lys Arg Gln Asn Asn Asn His Phe Ala His His Thr Cys Asn Lys         
145                 150                 155                 160         

tcg tgg cga tgc ggc att tcc act tcg aaa atg tac agc agg ctc gag      528
Ser Trp Arg Cys Gly Ile Ser Thr Ser Lys Met Tyr Ser Arg Leu Glu         
                165                 170                 175             

tgc cag gac gac acg gac gag tgc cag gta tac att ttg gac gct gag      576
Cys Gln Asp Asp Thr Asp Glu Cys Gln Val Tyr Ile Leu Asp Ala Glu         
            180                 185                 190                 

ggc aac ccc atc aac gtg acc gtg gac act gtg ctt cat cga gac ggc      624
Gly Asn Pro Ile Asn Val Thr Val Asp Thr Val Leu His Arg Asp Gly         
        195                 200                 205                     

gtg agt atg att ctc aaa caa aag tct acg ttc acc acg cgc caa ata      672
Val Ser Met Ile Leu Lys Gln Lys Ser Thr Phe Thr Thr Arg Gln Ile         
    210                 215                 220                         

aaa gct gcg tgt ctg ctc att aaa gat gac aaa aat aac ccc gag tcg      720
Lys Ala Ala Cys Leu Leu Ile Lys Asp Asp Lys Asn Asn Pro Glu Ser         
225                 230                 235                 240         

gtg aca cgc gaa cac tgt ttg att gac aat gat ata tat gat ctt tct      768
Val Thr Arg Glu His Cys Leu Ile Asp Asn Asp Ile Tyr Asp Leu Ser         
                245                 250                 255             

aaa aac acg tgg aac tgc aag ttt aac aga tgc att aaa cgc aaa gtc      816
Lys Asn Thr Trp Asn Cys Lys Phe Asn Arg Cys Ile Lys Arg Lys Val         
            260                 265                 270                 

gag cac cga gtc aag aag cgg ccg ccc act tgg cgc cac aac gtt aga      864
Glu His Arg Val Lys Lys Arg Pro Pro Thr Trp Arg His Asn Val Arg         
        275                 280                 285                     

gcc aag tac aca gag gga gac act gcc acc aaa ggc gac ctg atg cat      912
Ala Lys Tyr Thr Glu Gly Asp Thr Ala Thr Lys Gly Asp Leu Met His         
    290                 295                 300                         

att caa gag gag ctg atg tac gaa aac gat ttg ctg aaa atg aac att      960
Ile Gln Glu Glu Leu Met Tyr Glu Asn Asp Leu Leu Lys Met Asn Ile         
305                 310                 315                 320         

gag ctg atg cat gcg cac atc aac aag cta aac aat atg ctg cac gac     1008
Glu Leu Met His Ala His Ile Asn Lys Leu Asn Asn Met Leu His Asp         
                325                 330                 335             

ctg ata gtc tcc gtg gcc aag gtg gac gag cgt ttg att ggc aat ctc     1056
Leu Ile Val Ser Val Ala Lys Val Asp Glu Arg Leu Ile Gly Asn Leu         
            340                 345                 350                 

atg aac aac tct gtt tct tca aca ttt ttg tcg gac gac acg ttt ttg     1104
Met Asn Asn Ser Val Ser Ser Thr Phe Leu Ser Asp Asp Thr Phe Leu         
        355                 360                 365                     

ctg atg ccg tgc acc aat ccg ccg gca cac acc agt aat tgc tac aac     1152
Leu Met Pro Cys Thr Asn Pro Pro Ala His Thr Ser Asn Cys Tyr Asn         
    370                 375                 380                         

aac agc atc tac aaa gaa ggg cgt tgg gtg gcc aac acg gac tcg tcg     1200
Asn Ser Ile Tyr Lys Glu Gly Arg Trp Val Ala Asn Thr Asp Ser Ser         
385                 390                 395                 400         

caa tgc ata gat ttt agc aac tac aag gaa cta gca att gac gac gac     1248
Gln Cys Ile Asp Phe Ser Asn Tyr Lys Glu Leu Ala Ile Asp Asp Asp         
                405                 410                 415             

gtc gag ttt tgg atc ccg acc atc ggc aac acg acc tat cac gac agt     1296
Val Glu Phe Trp Ile Pro Thr Ile Gly Asn Thr Thr Tyr His Asp Ser         
            420                 425                 430                 

tgg aaa gat gcc agc ggc tgg tcg ttt att gcc caa caa aaa ggc aac     1344
Trp Lys Asp Ala Ser Gly Trp Ser Phe Ile Ala Gln Gln Lys Gly Asn         
        435                 440                 445                     

ctc ata acc acc atg gag aac acc aag ttt ggc ggc gtc ggc acc agt     1392
Leu Ile Thr Thr Met Glu Asn Thr Lys Phe Gly Gly Val Gly Thr Ser         
    450                 455                 460                         

ctg agc gac atc act tcc atg gct gaa ggc gaa ttg gcc gct aaa ttg     1440
Leu Ser Asp Ile Thr Ser Met Ala Glu Gly Glu Leu Ala Ala Lys Leu         
465                 470                 475                 480         

act tcg ttc atg ttt ggt cat gta gtt aac ttt gta att ata tta att     1488
Thr Ser Phe Met Phe Gly His Val Val Asn Phe Val Ile Ile Leu Ile         
                485                 490                 495             

gtg att tta ttt ttg tac tgt atg att aga aac cgt aat aga caa tat     1536
Val Ile Leu Phe Leu Tyr Cys Met Ile Arg Asn Arg Asn Arg Gln Tyr         
            500                 505                 510                 

taa                                                                 1539


<210>  2
<211>  512
<212>  PRT
<213>  Autographa californica nuclear polyhedrosis virus

<400>  2

Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala Ala His 
1               5                   10                  15      


Ser Ala Phe Ala Ala Glu His Cys Asn Ala Gln Met Lys Thr Gly Pro 
            20                  25                  30          


Tyr Lys Ile Lys Asn Leu Asp Ile Thr Pro Pro Lys Glu Thr Leu Gln 
        35                  40                  45              


Lys Asp Val Glu Ile Thr Ile Val Glu Thr Asp Tyr Asn Glu Asn Val 
    50                  55                  60                  


Ile Ile Gly Tyr Lys Gly Tyr Tyr Gln Ala Tyr Ala Tyr Asn Gly Gly 
65                  70                  75                  80  


Ser Leu Asp Pro Asn Thr Arg Val Glu Glu Thr Met Lys Thr Leu Asn 
                85                  90                  95      


Val Gly Lys Glu Asp Leu Leu Met Trp Ser Ile Arg Gln Gln Cys Glu 
            100                 105                 110         


Val Gly Glu Glu Leu Ile Asp Arg Trp Gly Ser Asp Ser Asp Asp Cys 
        115                 120                 125             


Phe Arg Asp Asn Glu Gly Arg Gly Gln Trp Val Lys Gly Lys Glu Leu 
    130                 135                 140                 


Val Lys Arg Gln Asn Asn Asn His Phe Ala His His Thr Cys Asn Lys 
145                 150                 155                 160 


Ser Trp Arg Cys Gly Ile Ser Thr Ser Lys Met Tyr Ser Arg Leu Glu 
                165                 170                 175     


Cys Gln Asp Asp Thr Asp Glu Cys Gln Val Tyr Ile Leu Asp Ala Glu 
            180                 185                 190         


Gly Asn Pro Ile Asn Val Thr Val Asp Thr Val Leu His Arg Asp Gly 
        195                 200                 205             


Val Ser Met Ile Leu Lys Gln Lys Ser Thr Phe Thr Thr Arg Gln Ile 
    210                 215                 220                 


Lys Ala Ala Cys Leu Leu Ile Lys Asp Asp Lys Asn Asn Pro Glu Ser 
225                 230                 235                 240 


Val Thr Arg Glu His Cys Leu Ile Asp Asn Asp Ile Tyr Asp Leu Ser 
                245                 250                 255     


Lys Asn Thr Trp Asn Cys Lys Phe Asn Arg Cys Ile Lys Arg Lys Val 
            260                 265                 270         


Glu His Arg Val Lys Lys Arg Pro Pro Thr Trp Arg His Asn Val Arg 
        275                 280                 285             


Ala Lys Tyr Thr Glu Gly Asp Thr Ala Thr Lys Gly Asp Leu Met His 
    290                 295                 300                 


Ile Gln Glu Glu Leu Met Tyr Glu Asn Asp Leu Leu Lys Met Asn Ile 
305                 310                 315                 320 


Glu Leu Met His Ala His Ile Asn Lys Leu Asn Asn Met Leu His Asp 
                325                 330                 335     


Leu Ile Val Ser Val Ala Lys Val Asp Glu Arg Leu Ile Gly Asn Leu 
            340                 345                 350         


Met Asn Asn Ser Val Ser Ser Thr Phe Leu Ser Asp Asp Thr Phe Leu 
        355                 360                 365             


Leu Met Pro Cys Thr Asn Pro Pro Ala His Thr Ser Asn Cys Tyr Asn 
    370                 375                 380                 


Asn Ser Ile Tyr Lys Glu Gly Arg Trp Val Ala Asn Thr Asp Ser Ser 
385                 390                 395                 400 


Gln Cys Ile Asp Phe Ser Asn Tyr Lys Glu Leu Ala Ile Asp Asp Asp 
                405                 410                 415     


Val Glu Phe Trp Ile Pro Thr Ile Gly Asn Thr Thr Tyr His Asp Ser 
            420                 425                 430         


Trp Lys Asp Ala Ser Gly Trp Ser Phe Ile Ala Gln Gln Lys Gly Asn 
        435                 440                 445             


Leu Ile Thr Thr Met Glu Asn Thr Lys Phe Gly Gly Val Gly Thr Ser 
    450                 455                 460                 


Leu Ser Asp Ile Thr Ser Met Ala Glu Gly Glu Leu Ala Ala Lys Leu 
465                 470                 475                 480 


Thr Ser Phe Met Phe Gly His Val Val Asn Phe Val Ile Ile Leu Ile 
                485                 490                 495     


Val Ile Leu Phe Leu Tyr Cys Met Ile Arg Asn Arg Asn Arg Gln Tyr 
            500                 505                 510         


<210>  3
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-F1

<400>  3
cacacaagca agatggtaag cgctattg                                        28


<210>  4
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-R2

<400>  4
gtgggcggcc gcttcttgac tcggtgc                                         27


<210>  5
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-F2

<400>  5
gcaccgagtc aagaagcggc cgcccac                                         27


<210>  6
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-R1

<400>  6
catttaatat tgtctattac ggtttc                                          26


<210>  7
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer for DNA for PCR; Primer M13 Forward

<400>  7
gtaaaacgac ggccagt                                                    17


<210>  8
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer M13 Reverse

<400>  8
ggaaacagct atgaccatg                                                  19


<210>  9
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-F3

<400>  9
gtcgagcacc gagtcaagaa gcggccgccc ac                                   32


<210>  10
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-R3

<400>  10
gatggggttg ccctcagcgt cc                                              22


<210>  11
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer pp34-F

<400>  11
atatttcaaa atatgaagcc gacg                                            24


<210>  12
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer pp34-R

<400>  12
gagttggtgt tgttcaactc gttgcgc                                         27


<210>  13
<211>  759
<212>  DNA
<213>  Autographa californica nuclear polyhedrosis virus

<220>
<221>  CDS
<222>  (1)..(759)
<223>  pp34 gene of Autographa californica nuclear polyhedrosis virus


<400>  13
atg aag ccg acg aat aac gtt atg ttc gac gac gcg tcg gtc ctt tgg       48
Met Lys Pro Thr Asn Asn Val Met Phe Asp Asp Ala Ser Val Leu Trp         
1               5                   10                  15              

atc gac acg gac tac att tat caa aat tta aaa atg cct ttg cag gcg       96
Ile Asp Thr Asp Tyr Ile Tyr Gln Asn Leu Lys Met Pro Leu Gln Ala         
            20                  25                  30                  

ttt caa caa ctt ttg ttc acc att cca tct aaa cat aga aaa atg atc      144
Phe Gln Gln Leu Leu Phe Thr Ile Pro Ser Lys His Arg Lys Met Ile         
        35                  40                  45                      

aac gat gcg ggc gga tcg tgt cat aac acg gtc aaa tac atg gtg gac      192
Asn Asp Ala Gly Gly Ser Cys His Asn Thr Val Lys Tyr Met Val Asp         
    50                  55                  60                          

att tac gga gcg gcc gtt ctg gtt ttg cga acg cct tgc tcg ttc gcc      240
Ile Tyr Gly Ala Ala Val Leu Val Leu Arg Thr Pro Cys Ser Phe Ala         
65                  70                  75                  80          

gac cag ttg ttg agc aca ttt att gca aac aat tat ttg tgc tac ttt      288
Asp Gln Leu Leu Ser Thr Phe Ile Ala Asn Asn Tyr Leu Cys Tyr Phe         
                85                  90                  95              

tac cgt cgt cgc cga tca cga tca cgc tca cga tca cgc tcg cga tca      336
Tyr Arg Arg Arg Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser         
            100                 105                 110                 

cgt tct cct cat tgc aga cct cgt tcg cgc tct cct cat tgc aga cct      384
Arg Ser Pro His Cys Arg Pro Arg Ser Arg Ser Pro His Cys Arg Pro         
        115                 120                 125                     

cgt tcg cga tct cgg tcc cgg tct aga tcg cgg tca cgt tca tcg tct      432
Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Ser Ser         
    130                 135                 140                         

ccc agg cga ggg cgt cga caa ata ttc gac gcg ctg gaa aag att cgt      480
Pro Arg Arg Gly Arg Arg Gln Ile Phe Asp Ala Leu Glu Lys Ile Arg         
145                 150                 155                 160         

cat caa aac gac atg ttg atg agc aac gtc aac caa ata aat ctc aac      528
His Gln Asn Asp Met Leu Met Ser Asn Val Asn Gln Ile Asn Leu Asn         
                165                 170                 175             

caa act aat caa ttt tta gaa ttg tcc aac atg atg acg ggc gtg cgc      576
Gln Thr Asn Gln Phe Leu Glu Leu Ser Asn Met Met Thr Gly Val Arg         
            180                 185                 190                 

aat caa aac gtg cag ctc ctc gcg gcg ttg gaa acc gct aaa gat gtt      624
Asn Gln Asn Val Gln Leu Leu Ala Ala Leu Glu Thr Ala Lys Asp Val         
        195                 200                 205                     

att ttg acc aga tta aac aca ttg ctt gcc gag att aca gac tcg tta      672
Ile Leu Thr Arg Leu Asn Thr Leu Leu Ala Glu Ile Thr Asp Ser Leu         
    210                 215                 220                         

ccc gac ttg acg tcc atg tta gat aaa tta gct gaa caa ttg ttg gac      720
Pro Asp Leu Thr Ser Met Leu Asp Lys Leu Ala Glu Gln Leu Leu Asp         
225                 230                 235                 240         

gcc atc aac acg gtg cag caa acc tgc gca acg agt tga                  759
Ala Ile Asn Thr Val Gln Gln Thr Cys Ala Thr Ser                         
                245                 250                                 


<210>  14
<211>  252
<212>  PRT
<213>  Autographa californica nuclear polyhedrosis virus

<400>  14

Met Lys Pro Thr Asn Asn Val Met Phe Asp Asp Ala Ser Val Leu Trp 
1               5                   10                  15      


Ile Asp Thr Asp Tyr Ile Tyr Gln Asn Leu Lys Met Pro Leu Gln Ala 
            20                  25                  30          


Phe Gln Gln Leu Leu Phe Thr Ile Pro Ser Lys His Arg Lys Met Ile 
        35                  40                  45              


Asn Asp Ala Gly Gly Ser Cys His Asn Thr Val Lys Tyr Met Val Asp 
    50                  55                  60                  


Ile Tyr Gly Ala Ala Val Leu Val Leu Arg Thr Pro Cys Ser Phe Ala 
65                  70                  75                  80  


Asp Gln Leu Leu Ser Thr Phe Ile Ala Asn Asn Tyr Leu Cys Tyr Phe 
                85                  90                  95      


Tyr Arg Arg Arg Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser 
            100                 105                 110         


Arg Ser Pro His Cys Arg Pro Arg Ser Arg Ser Pro His Cys Arg Pro 
        115                 120                 125             


Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Ser Ser 
    130                 135                 140                 


Pro Arg Arg Gly Arg Arg Gln Ile Phe Asp Ala Leu Glu Lys Ile Arg 
145                 150                 155                 160 


His Gln Asn Asp Met Leu Met Ser Asn Val Asn Gln Ile Asn Leu Asn 
                165                 170                 175     


Gln Thr Asn Gln Phe Leu Glu Leu Ser Asn Met Met Thr Gly Val Arg 
            180                 185                 190         


Asn Gln Asn Val Gln Leu Leu Ala Ala Leu Glu Thr Ala Lys Asp Val 
        195                 200                 205             


Ile Leu Thr Arg Leu Asn Thr Leu Leu Ala Glu Ile Thr Asp Ser Leu 
    210                 215                 220                 


Pro Asp Leu Thr Ser Met Leu Asp Lys Leu Ala Glu Gln Leu Leu Asp 
225                 230                 235                 240 


Ala Ile Asn Thr Val Gln Gln Thr Cys Ala Thr Ser 
                245                 250         


<210>  15
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer HVT45Sph

<400>  15
gggttatagt tgcatgcgcg tggccggga                                       29


<210>  16
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer 45SfiR

<400>  16
ggccgggggg gccgttagag gtgcgttttt                                      30


<210>  17
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer 46SfiF

<400>  17
cccccccggc cttactgtgt tttatttatc                                      30


<210>  18
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer HVT46Xho

<400>  18
catggggggc tctcgagtgc agcataaa                                        28


<210>  19
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotides used for plasmid construction; Oligonucleotide S
       fi-polA-Sal-Pst-Sfi-U

<400>  19
cggcttttta tttatttagg tatgctgggg gtcgacggac tgcaggcccc cc             52


<210>  20
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotides used for plasmid construction; Oligonucleotide S
       fi-polA-Sal-Pst-Sfi-L

<400>  20
gggcctgcag tccgtcgacc cccagcatac ctaaataaat aaaaagccgg gg             52


<210>  21
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-Xba-F

<400>  21
cgcccttcac acaagtctag aatggtaagc gctattg                              37


<210>  22
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-Sal-R

<400>  22
gcagaattcg gtcgactatt aatattgtc                                       29


<210>  23
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer for DNA for PCR; Primer BamHA-F

<400>  23
tgacggatcc atggaaagaa tagtgattg                                       29


<210>  24
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer for DNA for PCR; Primer SalHA-R

<400>  24
ctgacagtcg acctagatgc aaattctgc                                       29


<210>  25
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer HA-F

<400>  25
ctggacaata ctaaggccga acgat                                           25


<210>  26
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer HA-R

<400>  26
cactggggtc tgacatttgg ta                                              22


<210>  27
<211>  1695
<212>  DNA
<213>  Influenza A virus

<220>
<221>  CDS
<222>  (1)..(1695)
<223>  Hemagglutinin gene of A/Turkey/Wisconsin/68 (H5N9)


<400>  27
atg gaa aga ata gtg att gcc ctt gca ata atc agc gtt gtc aaa ggt       48
Met Glu Arg Ile Val Ile Ala Leu Ala Ile Ile Ser Val Val Lys Gly         
1               5                   10                  15              

gac caa atc tgc atc ggt tat cat gca aac aat tca aca aaa caa gtt       96
Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Lys Gln Val         
            20                  25                  30                  

gac aca atc atg gag aag aat gtg acg gtc aca cat gct caa gat ata      144
Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile         
        35                  40                  45                      

ctg gaa aaa gag cac aac ggg aaa ctc tgc agt ctc aaa gga gtg agg      192
Leu Glu Lys Glu His Asn Gly Lys Leu Cys Ser Leu Lys Gly Val Arg         
    50                  55                  60                          

ccc ctc att ctg aag gat tgc agt gtg gct gga tgg ctt ctt ggg aac      240
Pro Leu Ile Leu Lys Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn         
65                  70                  75                  80          

cca atg tgt gat gag ttc cta aat gta ccg gaa tgg tca tat att gta      288
Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val         
                85                  90                  95              

gag aag gac aat cca acc aat ggc tta tgt tat ccg gga gac ttc aat      336
Glu Lys Asp Asn Pro Thr Asn Gly Leu Cys Tyr Pro Gly Asp Phe Asn         
            100                 105                 110                 

gat tat gaa gaa ctg aag tat tta atg agc aac aca aac cat ttt gag      384
Asp Tyr Glu Glu Leu Lys Tyr Leu Met Ser Asn Thr Asn His Phe Glu         
        115                 120                 125                     

aaa att caa ata atc cct agg aac tct tgg tcc aat cat gat gcc tca      432
Lys Ile Gln Ile Ile Pro Arg Asn Ser Trp Ser Asn His Asp Ala Ser         
    130                 135                 140                         

tca gga gtg agc tca gca tgc cca tac aat ggt aga tct tcc ttt ttc      480
Ser Gly Val Ser Ser Ala Cys Pro Tyr Asn Gly Arg Ser Ser Phe Phe         
145                 150                 155                 160         

agg aat gtg gtg tgg ttg atc aag aag agt aat gca tac cca aca ata      528
Arg Asn Val Val Trp Leu Ile Lys Lys Ser Asn Ala Tyr Pro Thr Ile         
                165                 170                 175             

aag agg acc tac aat aac acc aat gta gag gac ctt ctg ata ttg tgg      576
Lys Arg Thr Tyr Asn Asn Thr Asn Val Glu Asp Leu Leu Ile Leu Trp         
            180                 185                 190                 

gga atc cat cac cct aat gat gca gcg gaa caa acg gaa ctc tat cag      624
Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Glu Leu Tyr Gln         
        195                 200                 205                     

aac tcg aac act tat gtg tct gta gga aca tca aca cta aat cag agg      672
Asn Ser Asn Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Gln Arg         
    210                 215                 220                         

tca att cca gaa ata gct acc agg ccc aaa gtg aat gga caa agt gga      720
Ser Ile Pro Glu Ile Ala Thr Arg Pro Lys Val Asn Gly Gln Ser Gly         
225                 230                 235                 240         

aga ata gaa ttt ttc tgg aca ata cta agg ccg aac gat gca atc agc      768
Arg Ile Glu Phe Phe Trp Thr Ile Leu Arg Pro Asn Asp Ala Ile Ser         
                245                 250                 255             

ttt gaa agt aat ggg aac ttt ata gct cct gaa tat gca tac aag ata      816
Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile         
            260                 265                 270                 

gtt aaa aag gga gat tca gca atc atg aga agc gaa ctg gag tat ggc      864
Val Lys Lys Gly Asp Ser Ala Ile Met Arg Ser Glu Leu Glu Tyr Gly         
        275                 280                 285                     

aac tgt gat acc aaa tgt cag acc cca gtg ggt gct ata aat tcc agt      912
Asn Cys Asp Thr Lys Cys Gln Thr Pro Val Gly Ala Ile Asn Ser Ser         
    290                 295                 300                         

atg cct ttt cac aat gtt cat ccc ctt acc att gga gag tgt ccc aaa      960
Met Pro Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys         
305                 310                 315                 320         

tat gtc aaa tca gat aaa ctg gtc ctt gca aca gga ctg agg aac gtg     1008
Tyr Val Lys Ser Asp Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Val         
                325                 330                 335             

cct cag aga gaa aca aga ggt ctg ttt gga gca ata gca gga ttc ata     1056
Pro Gln Arg Glu Thr Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile         
            340                 345                 350                 

gaa ggg ggg tgg caa gga atg gta gat gga tgg tat ggt tac cat cat     1104
Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His         
        355                 360                 365                     

agc aac gag cag gga agt gga tat gct gca gac aaa gag tcc act cag     1152
Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln         
    370                 375                 380                         

aaa gca atc gac ggg atc acc aat aaa gtc aac tca atc att gac aaa     1200
Lys Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Ile Ile Asp Lys         
385                 390                 395                 400         

atg aac act caa ttc gaa gcc gtt ggg aaa gaa ttc aac aac tta gaa     1248
Met Asn Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Asn Asn Leu Glu         
                405                 410                 415             

agg aga ata gaa aat ttg aat aag aaa atg gaa gat gga ttt cta gat     1296
Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp         
            420                 425                 430                 

gta tgg act tac aat gca gaa ctt ctg gtg ctc atg gaa aat gaa aga     1344
Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg         
        435                 440                 445                     

act ctg gat ttc cat gat tca tat gtc aag aac cta tac gat aag gtc     1392
Thr Leu Asp Phe His Asp Ser Tyr Val Lys Asn Leu Tyr Asp Lys Val         
    450                 455                 460                         

cga ctc cag ctg aga gat aat gca aaa gaa ttg ggc aat ggg tgt ttt     1440
Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe         
465                 470                 475                 480         

gag ttc tac cac aaa tgt gac aat gaa tgc atg gaa agt gtg aga aac     1488
Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn         
                485                 490                 495             

gga acg tat gac tat cca caa tac tca gaa gaa tca agg ctg aac aga     1536
Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ser Arg Leu Asn Arg         
            500                 505                 510                 

gag gaa ata gat gga gtc aaa ttg gag tca atg ggc acc tat cag ata     1584
Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Met Gly Thr Tyr Gln Ile         
        515                 520                 525                     

cta tca atc tac tca aca gtg gcg agt tcc cta gca ctg gca atc atg     1632
Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met         
    530                 535                 540                         

gta gct ggt ctg tct ttt tgg atg tgc tcc aat gga tca ttg cag tgc     1680
Val Ala Gly Leu Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys         
545                 550                 555                 560         

aga att tgc atc tag                                                 1695
Arg Ile Cys Ile                                                         
                                                                        


<210>  28
<211>  564
<212>  PRT
<213>  Influenza A virus

<400>  28

Met Glu Arg Ile Val Ile Ala Leu Ala Ile Ile Ser Val Val Lys Gly 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Lys Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Glu His Asn Gly Lys Leu Cys Ser Leu Lys Gly Val Arg 
    50                  55                  60                  


Pro Leu Ile Leu Lys Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Asp Asn Pro Thr Asn Gly Leu Cys Tyr Pro Gly Asp Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys Tyr Leu Met Ser Asn Thr Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Arg Asn Ser Trp Ser Asn His Asp Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Asn Gly Arg Ser Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Ser Asn Ala Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Thr Tyr Asn Asn Thr Asn Val Glu Asp Leu Leu Ile Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Glu Leu Tyr Gln 
        195                 200                 205             


Asn Ser Asn Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Ser Ile Pro Glu Ile Ala Thr Arg Pro Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Ile Glu Phe Phe Trp Thr Ile Leu Arg Pro Asn Asp Ala Ile Ser 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Met Arg Ser Glu Leu Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asp Thr Lys Cys Gln Thr Pro Val Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asp Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Val 
                325                 330                 335     


Pro Gln Arg Glu Thr Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile 
            340                 345                 350         


Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His 
        355                 360                 365             


Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln 
    370                 375                 380                 


Lys Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Ile Ile Asp Lys 
385                 390                 395                 400 


Met Asn Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Asn Asn Leu Glu 
                405                 410                 415     


Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp 
            420                 425                 430         


Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg 
        435                 440                 445             


Thr Leu Asp Phe His Asp Ser Tyr Val Lys Asn Leu Tyr Asp Lys Val 
    450                 455                 460                 


Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe 
465                 470                 475                 480 


Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn 
                485                 490                 495     


Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ser Arg Leu Asn Arg 
            500                 505                 510         


Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Met Gly Thr Tyr Gln Ile 
        515                 520                 525             


Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met 
    530                 535                 540                 


Val Ala Gly Leu Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys 
545                 550                 555                 560 


Arg Ile Cys Ile 
                


<210>  29
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMV1

<400>  29
gggctgcaga gttattaata gtaatcaatt                                      30


<210>  30
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMV3

<400>  30
gggtcgttgg gcggtcagcc ggcgg                                           25


<210>  31
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMV4

<400>  31
cttacggtaa atggcccgcc ggctg                                           25


<210>  32
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMV5

<400>  32
tacacttgat gtactgccaa tgggc                                           25


<210>  33
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMV6

<400>  33
tatttacggt aaactgccca ttggc                                           25


<210>  34
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMV2'

<400>  34
cgcgccattt accgtcattg acgtc                                           25


<210>  35
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMVo1

<400>  35
acgtcaatga cggtaaatgg cgcgcctggc                                      30


<210>  36
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMVR1

<400>  36
cgtctagagg atctgacggt tcactaaacc                                      30


<210>  37
<211>  587
<212>  DNA
<213>  Cytomegalovirus

<220>
<221>  promoter
<222>  (1)..(587)
<223>  Cytomegalovirus immediate early promoter


<400>  37
agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc     60

gttacataac ttacggtaaa tggcccgccg gctgaccgcc caacgacccc cgcccattga    120

cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat    180

gggtggagta tttacggtaa actgcccatt ggcagtacat caagtgtatc atatgccaag    240

tacgccccct attgacgtca atgacggtaa atggcgcgcc tggcattatg cccagtacat    300

gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat    360

ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt    420

tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga    480

ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg    540

gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatcc                  587


<210>  38
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PolyA-SalKpn

<400>  38
tgtggtaccg tcgacgattc acagtcccaa ggc                                  33


<210>  39
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PolyA-SfiF2

<400>  39
cttggcctta ttggcctaag atacattgat gag                                  33


<210>  40
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PrCMVlBgl

<400>  40
ggtgtgtttt ggcccccccg gctgcagagt tattaatag                            39


<210>  41
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer PolA-SfiR

<400>  41
ttggccttat tggccgattc acagtcccaa gg                                   32


<210>  42
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer HA2-P-F

<400>  42
ccaaatctgc atcggttatc at                                              22


<210>  43
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer HA2-P-R

<400>  43
gtttgttccg ctgcatcatt ag                                              22


<210>  44
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-F

<400>  44
cgctgcaaaa ggacgtggaa atca                                            24


<210>  45
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer GP64-R

<400>  45
caccttggcc acggagacta t                                               21


<210>  46
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer 45/46-F

<400>  46
tagcggcacg gaaacagata gaga                                            24


<210>  47
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer DNA for PCR; Primer 45/46-R

<400>  47
tggcgatacg gttcctggtt tgac                                            24

