
                                SEQUENCE LISTING

<110> Pioneer Hi-Bred International, Inc.

<120> Use of Virus-Induced Gene Silencing
  (VIGS) to Down-Regulate Genes in Plants
  

<130> 2483-PCT

<150> US 61/042,815       
<151> 2008-04-07  

<160> 46

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC1F
      

<400> 1
actgtcgaat tcgccagatg gagaagagta atctt                            35

<210> 2
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC1R
      

<400> 2
ctatgcaagc ttgagccgga gagagagtat agattat                          37

<210> 3
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC2F
      

<400> 3
actgtcctgc agctagccag atggagaaga ataatc                           36

<210> 4
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC2R
      

<400> 4
ctatgcaagc ttgatataca acgttcaccc ttatagg                          37

<210> 5
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC3F
      

<400> 5
actgtcctgc aggctgaaag aagagaatca ggc                              33

<210> 6
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC3R
      

<400> 6
ctatgcatcg atctcagcca agggagtatt gag                              33

<210> 7
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC4F
      

<400> 7
actgtcctgc agctagccag atggagaaga ataatc                           36

<210> 8
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC4R
      

<400> 8
ctatgcaagc ttaagagagt gtgaagatat acaacg                           36

<210> 9
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC5F
      

<400> 9
actgtcctgc agccgaagct gataatatag agatgtc                          37

<210> 10
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer VirFLC5R
      

<400> 10
ctatgcaagc ttgggttaaa ctgacatagg ttatttg                          37

<210> 11
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer Consensus FLCLong5'
      

<400> 11
gccctctccg taactagagc taggaagaca gaactaatgt tgaagcttgt gatagcc    57

<210> 12
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer ConsensusFLCLong3'
      

<400> 12
tggttctctt ctttcagcaa attctccttt tctttgaggc tatcacaagc ttcaacatta 60
gttctg                                                            66

<210> 13
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer ConsensusFLCF
      

<400> 13
actgtcctgc aggccctctc cgtaactaga gc                               32

<210> 14
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer ConsensusFLCR
      

<400> 14
ctatgcatcg attggttctc ttctttcagc aa                               32

<210> 15
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer CLCV-BGenF
      

<400> 15
ggatctacca cgatatctaa taggc                                       25

<210> 16
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer CLCV-BGenR
      

<400> 16
acagagttag cgacacaaat gtg                                         23

<210> 17
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer CLCV-AGenF
      

<400> 17
aataaagacg acgtctacca caac                                        24

<210> 18
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer CLCV-AGenR
      

<400> 18
tcttgtgctg tgctttgata gag                                         23

<210> 19
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC1F
      

<400> 19
gccagatgga gaagagtaat ctt                                         23

<210> 20
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC1R
      

<400> 20
gagccggaga gagagtatag attat                                       25

<210> 21
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC2F
      

<400> 21
ctagccagat ggagaagaat aatc                                        24

<210> 22
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC2R
      

<400> 22
gatatacaac gttcaccctt atagg                                       25

<210> 23
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC3F
      

<400> 23
gctgaaagaa gagaatcagg c                                           21

<210> 24
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC3R
      

<400> 24
ctcagccaag ggagtattga g                                           21

<210> 25
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC4F
      

<400> 25
ctagccagat ggagaagaat aatc                                        24

<210> 26
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC4R
      

<400> 26
aagagagtgt gaagatatac aacgc                                       25

<210> 27
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC5F
      

<400> 27
ccgaagctga taatatagag atgtc                                       25

<210> 28
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer BnFLC5R
      

<400> 28
gggttaaact gacataggtt atttg                                       25

<210> 29
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer PDSF
      

<400> 29
gatataccaa ggccagagct aga                                         23

<210> 30
<211> 226
<212> DNA
<213> Brassica napus

<220> 
<221> misc_feature   
<222> (1)...(226)
<223> FLC1 fragment
      

<400> 30
gccagatgga gaagagtaat cttgtgcgag ccgaagctga taatatggat gtctcaccag 60
gacaaatctc cgacatcaat cttccggtaa cgctcccact gcttaattag tcacctttaa 120
tcggcgaata aataaaatcc aaaacatata actaaaacaa acaagatgtg taattatccc 180
cttgtaaagg gtgtacgttg tataatctat actctctctc cggctc                226

<210> 31
<211> 194
<212> DNA
<213> Brassica napus

<220> 
<221> misc_feature   
<222> (1)...(194)
<223> BnFLC2 fragment

<400> 31
ctagccagat ggagaagaat aatcttgcgg gagccgaagc tgataaaatg gagatgtcac 60
ctggacaaat ctctgacatc aatcgtccgg taactctccg actgctttat tagccacctt 120
aagtccaaaa cttgtgacta aaaacaaaaa taagttatcg aactattccc ctataagggt 180
gaacgttgta tatc                                                   194

<210> 32
<211> 260
<212> DNA
<213> Brassica napus

<220> 
<221> misc_feature   
<222> (1)...(260)
<223> FLC3 fragment

<400> 32
gctgaaagaa gagaatcagg ctttggctgg ccagaaggag aagaagaatc ttgcgggagc 60
cgaagctgat aatatggaga tgtcacctgg acaaatctcc gacatcaatc ttccggtaac 120
tctcccactg cttaattagc caccgttaga cggggctgat caaattaaaa aatccaaaac 180
atacaactaa ataaataagc tttgttgttt ttcacccttg aagggtgcac gttgtatatc 240
tcaatactcc cttggctgag                                             260

<210> 33
<211> 206
<212> DNA
<213> Brassica napus

<220> 
<221> misc_feature   
<222> (1)...(206)
<223> FLC4

<400> 33
ctagccagat ggagaagaat aatcttgcgg gagccgaagc tgataaaatg gaagtgtcac 60
ctggacagat ctctgacatc aattgtccgg taactctccc actgctttat tagcccctta 120
agtccaaaac ttgtgactaa aaacaaaaat aagttaccga actattcccc tataagggtg 180
agcgttgtat atcttcacac tctctt                                      206

<210> 34
<211> 236
<212> DNA
<213> Brassica napus

<220> 
<221> misc_feature   
<222> (1)...(236)
<223> FLC5

<220> 
<221> misc_feature   
<222> 169
<223> n = A,T,C or G

<400> 34
ccgaagctga taatatagag atgtcatctg gacaaatctc cgacatcaat cttcctgtaa 60
ctctcccgct gcttaattaa ccacctttac tcggcggtta atcaaaataa gaaacatata 120
atctaaagat aacctatgta ggttttactt ttcgcagctt aattaaccnc ctttactcgg 180
cggttaatcg aaattaaaaa catataatta acaaataacc tatgtcagtt taaccc     236

<210> 35
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<223> BnFLC1-5 consensus
      

<400> 35
gccctctccg taactagagc taggaagaca gaactaatgt tgaagcttgt tgatagcctc 60
aaagaaaagg agaatttgct gaaagaagag aacca                            95

<210> 36
<211> 6224
<212> DNA
<213> Artificial Sequence

<220> 
<223> Viral vector CaLCuV A Plus FLC1
      

<400> 36
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60
attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120
gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180
caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240
ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300
cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360
agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420
cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480
caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540
gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600
taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 660
gccccccctc gaggttaacc caagcagcgg ccttatcaaa tttatggatc atacctgcac 720
gaggtgaata gttagcattg cgggacactt tagaggtccc cgccatagaa cgccacgggg 780
catcccgctt aggcatttcg aagtaaagca taaattgaaa tcacatgtct ttatataggc 840
ccacagcgca ctcagttagc cttaatagct aaccttgtca ggcggcatgt gattggttag 900
atttcattta atgcgtggtc ccacgcaaga caccaaaaaa atcgcggcca tccggtaata 960
ttattgcgga tggccgcttt tgcccctggt gttccccaga ggcttttagt atatattgga 1020
caccaagaca ccaaaaacaa taggttttag agagagctct cctggcgtcc tcccctggag 1080
tcctaaatat tatttacaaa aatgccacga aaccctaaat cgtttcgttt agcagcccga 1140
aatatattct taacatatcc ccagtgcgac atacccaaag atgaagctct tcagatgctt 1200
caaaccctgt cgtggtcagt cgtcaaaccc acatacatca gagtcgcaag agaggaacat 1260
tcagacgggt tcccccattt acactgtctc atccaactat caggaaagtc gaacatcaag 1320
gatgctagat ttttcgacat cactcacccc agaaggtctg ccaattttca cccaaacatt 1380
caggcagcca aagacaccaa tgccgtcaag aattacatca ccaaagatgg tgattattgt 1440
gaatccgggc agtacaaggt gtctgggggt acaaaggcaa ataaagacga cgtctaccac 1500
aacgccgtca atgcgggatg tgtggaagag gctctcgcaa ttataagggc tggagatcca 1560
aagacgttca ttgttagtta tcataatgtt agagctaaca tagagcgact ctttactaag 1620
gctccggaac catgggctcc tccgtttcaa ctctcctcct ttactaacgt cccggacgag 1680
atgagttcat gggcagatga ctattttggt cggagtgccg ctgcgcgggc ggaaagacct 1740
attagtatca tagttgaagg tgattcacga accggcaaga ccatgtgggc gcgtgcttta 1800
ggaccacata attatttgag tgggcacctc gactttaatt caaaggtctt ttcaaataat 1860
gcggagtata acgtcattga tgacatagct ccgcattatc taaagctaaa gcactggaaa 1920
gagcttattg gggcccaaag ggactggcaa tcaaactgta agtacggcaa gccagttcaa 1980
attaaaggtg gcataccctc aatcgtgctg tgcaatccag gagaggggag cagttatata 2040
agtttcctca acaaagagga aaatgcatca ctaagagcgt ggactaccaa aaatgcaaaa 2100
ttcatcactc ttgaagcccc cctctatcaa agcacagcac aagattgcta aaaggagagc 2160
tgtacgccga agacgcattg atttgaactg cgggtgttct atcttcctcc acatcaattg 2220
cgcagataat ggattcacgc accggggaga gcatcactgt agctcaggca gagaattccg 2280
tttttatctg ggaggttcca aatcccctat atttcaggat acaacgcgta gaggacccgt 2340
tgtacaccag aaccaggatc taccacatcc aagtccggtt caaccacaac ctacggaaag 2400
cattggatct ccgcaaagcc tacttcaact tccaagtctg gacgacttcg atgagagctt 2460
ctgggccgac atatttaagt agattcaaat gccttgtaat gtctcattta gataacctag 2520
gtgttattgg tattaatcat gtaattagag ctgttcgttt cgcaacggac agatcttatg 2580
taactcatgt tcacgagaat catgtaataa atttcaaaat ttattaattt gttatcgaat 2640
cgtaaaaata gatccgaatt tttaatgtcg catacacagg attagaggca tgagtacatg 2700
ccatatacaa taacagagcg ttctccgtat gattctcgta tttccctgct tcttgatggt 2760
tgtacacaac gtagttattg accttccaaa aacgcttcac caacgcctgc tcgttactcg 2820
catactgccc accggttacc tttgcgtaaa acttgtgcat gacttgataa cgatcacgaa 2880
gatcgttctt cacagtagca gtgacggtat cgataagctt gagccggaga gagagtatag 2940
attatacaac gtacaccctt tacaagggga taattacaca tcttgtttgt tttagttata 3000
tgttttggat tttatttatt cgccgattaa aggtgactaa ttaagcagtg ggagcgttac 3060
cggaagattg atgtcggaga tttgtcctgg tgagacatcc atattatcag cttcggctcg 3120
cacaagatta ctcttctcca tctggcgaat tcctgcagcc caacccaagc agcggcctta 3180
tcaaatttat ggatcatacc tgcacgaggt gaatagttag cattgcggga cactttagag 3240
gtccccgcca tagaacgcca cggggcatcc cgcttaggca tttcgaagta aagcataaat 3300
tgaaatcaca tgtctttata taggcccaca gcgcactcag ttagccttaa tagctaacct 3360
tgtcaggcgg catgtgattg gttagatttc atttaatgcg tggtcccacg caagacacca 3420
aaaaaatcgc ggccatccgg taatattatt gcggatggcc gcttttgccc ctggtgttcc 3480
ccagaggctt ttagtatata ttggacacca agacaccaaa aacaataggt tttagagaga 3540
gctctcctgg cgtcctcccc tggagtccta aatattattt acaaaaatgc cacgaaaccc 3600
taaatcgttt cgtttagcag cccgaaatat attcttaaca tatccccagt gcgacatacc 3660
caaagatgaa gctcttcaga tgcttcaaac cctgtcgtgg tcagtcgtca aacccacata 3720
catcagagtc gcaagagagg aacattcaga cgggttcccc catttacact gtctcatcca 3780
actatcagga aagtcgaaca tcaaggatgc tagatttttc gacatcactc accccagaag 3840
gtctgccaat tttcacccaa acattcaggc agccaaagac accaatgccg tcaagaatta 3900
catcaccaaa gatggtgatt attgtgaatc cgggcagtac aaggtgtctg ggggtacaaa 3960
ggcaaataaa gacgacgtgg gggatccact agttctagag cggccgccac cgcggtggag 4020
ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata 4080
gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 4140
cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 4200
ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 4260
acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 4320
gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 4380
gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 4440
ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 4500
cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 4560
ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 4620
taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 4680
ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 4740
ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 4800
aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 4860
tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 4920
agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 4980
ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 5040
tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 5100
tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 5160
cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 5220
aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 5280
atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 5340
cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 5400
tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 5460
atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 5520
taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 5580
tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 5640
gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 5700
cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 5760
cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 5820
gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 5880
aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 5940
accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 6000
ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 6060
gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 6120
aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 6180
taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac                  6224



<210> 37
<211> 6228
<212> DNA
<213> Artificial Sequence

<220> 
<223> Viral vector CaLCuV A Plus FLC5
      

<220> 
<221> misc_feature   
<222> 2988
<223> n = A,T,C or G

<400> 37
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60
attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120
gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180
caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240
ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300
cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360
agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420
cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480
caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540
gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600
taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 660
gccccccctc gaggttaacc caagcagcgg ccttatcaaa tttatggatc atacctgcac 720
gaggtgaata gttagcattg cgggacactt tagaggtccc cgccatagaa cgccacgggg 780
catcccgctt aggcatttcg aagtaaagca taaattgaaa tcacatgtct ttatataggc 840
ccacagcgca ctcagttagc cttaatagct aaccttgtca ggcggcatgt gattggttag 900
atttcattta atgcgtggtc ccacgcaaga caccaaaaaa atcgcggcca tccggtaata 960
ttattgcgga tggccgcttt tgcccctggt gttccccaga ggcttttagt atatattgga 1020
caccaagaca ccaaaaacaa taggttttag agagagctct cctggcgtcc tcccctggag 1080
tcctaaatat tatttacaaa aatgccacga aaccctaaat cgtttcgttt agcagcccga 1140
aatatattct taacatatcc ccagtgcgac atacccaaag atgaagctct tcagatgctt 1200
caaaccctgt cgtggtcagt cgtcaaaccc acatacatca gagtcgcaag agaggaacat 1260
tcagacgggt tcccccattt acactgtctc atccaactat caggaaagtc gaacatcaag 1320
gatgctagat ttttcgacat cactcacccc agaaggtctg ccaattttca cccaaacatt 1380
caggcagcca aagacaccaa tgccgtcaag aattacatca ccaaagatgg tgattattgt 1440
gaatccgggc agtacaaggt gtctgggggt acaaaggcaa ataaagacga cgtctaccac 1500
aacgccgtca atgcgggatg tgtggaagag gctctcgcaa ttataagggc tggagatcca 1560
aagacgttca ttgttagtta tcataatgtt agagctaaca tagagcgact ctttactaag 1620
gctccggaac catgggctcc tccgtttcaa ctctcctcct ttactaacgt cccggacgag 1680
atgagttcat gggcagatga ctattttggt cggagtgccg ctgcgcgggc ggaaagacct 1740
attagtatca tagttgaagg tgattcacga accggcaaga ccatgtgggc gcgtgcttta 1800
ggaccacata attatttgag tgggcacctc gactttaatt caaaggtctt ttcaaataat 1860
gcggagtata acgtcattga tgacatagct ccgcattatc taaagctaaa gcactggaaa 1920
gagcttattg gggcccaaag ggactggcaa tcaaactgta agtacggcaa gccagttcaa 1980
attaaaggtg gcataccctc aatcgtgctg tgcaatccag gagaggggag cagttatata 2040
agtttcctca acaaagagga aaatgcatca ctaagagcgt ggactaccaa aaatgcaaaa 2100
ttcatcactc ttgaagcccc cctctatcaa agcacagcac aagattgcta aaaggagagc 2160
tgtacgccga agacgcattg atttgaactg cgggtgttct atcttcctcc acatcaattg 2220
cgcagataat ggattcacgc accggggaga gcatcactgt agctcaggca gagaattccg 2280
tttttatctg ggaggttcca aatcccctat atttcaggat acaacgcgta gaggacccgt 2340
tgtacaccag aaccaggatc taccacatcc aagtccggtt caaccacaac ctacggaaag 2400
cattggatct ccgcaaagcc tacttcaact tccaagtctg gacgacttcg atgagagctt 2460
ctgggccgac atatttaagt agattcaaat gccttgtaat gtctcattta gataacctag 2520
gtgttattgg tattaatcat gtaattagag ctgttcgttt cgcaacggac agatcttatg 2580
taactcatgt tcacgagaat catgtaataa atttcaaaat ttattaattt gttatcgaat 2640
cgtaaaaata gatccgaatt tttaatgtcg catacacagg attagaggca tgagtacatg 2700
ccatatacaa taacagagcg ttctccgtat gattctcgta tttccctgct tcttgatggt 2760
tgtacacaac gtagttattg accttccaaa aacgcttcac caacgcctgc tcgttactcg 2820
catactgccc accggttacc tttgcgtaaa acttgtgcat gacttgataa cgatcacgaa 2880
gatcgttctt cacagtagca gtgacggtat cgataagctt gggttaaact gacataggtt 2940
atttgttaat tatatgtttt taatttcgat taaccgccga gtaaaggngg ttaattaagc 3000
tgcgaaaagt aaaacctaca taggttatct ttagattata tgtttcttat tttgattaac 3060
cgccgagtaa aggtggttaa ttaagcagcg ggagagttac aggaagattg atgtcggaga 3120
tttgtccaga tgacatctct atattatcag cttcggctgc agcccaaccc aagcagcggc 3180
cttatcaaat ttatggatca tacctgcacg aggtgaatag ttagcattgc gggacacttt 3240
agaggtcccc gccatagaac gccacggggc atcccgctta ggcatttcga agtaaagcat 3300
aaattgaaat cacatgtctt tatataggcc cacagcgcac tcagttagcc ttaatagcta 3360
accttgtcag gcggcatgtg attggttaga tttcatttaa tgcgtggtcc cacgcaagac 3420
accaaaaaaa tcgcggccat ccggtaatat tattgcggat ggccgctttt gcccctggtg 3480
ttccccagag gcttttagta tatattggac accaagacac caaaaacaat aggttttaga 3540
gagagctctc ctggcgtcct cccctggagt cctaaatatt atttacaaaa atgccacgaa 3600
accctaaatc gtttcgttta gcagcccgaa atatattctt aacatatccc cagtgcgaca 3660
tacccaaaga tgaagctctt cagatgcttc aaaccctgtc gtggtcagtc gtcaaaccca 3720
catacatcag agtcgcaaga gaggaacatt cagacgggtt cccccattta cactgtctca 3780
tccaactatc aggaaagtcg aacatcaagg atgctagatt tttcgacatc actcacccca 3840
gaaggtctgc caattttcac ccaaacattc aggcagccaa agacaccaat gccgtcaaga 3900
attacatcac caaagatggt gattattgtg aatccgggca gtacaaggtg tctgggggta 3960
caaaggcaaa taaagacgac gtgggggatc cactagttct agagcggccg ccaccgcggt 4020
ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt 4080
catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 4140
gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 4200
tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 4260
gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 4320
actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 4380
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 4440
aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 4500
ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 4560
aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 4620
cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 4680
cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 4740
aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 4800
cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 4860
ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 4920
ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 4980
gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 5040
agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 5100
acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 5160
tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 5220
agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 5280
gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 5340
agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 5400
cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 5460
ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 5520
cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 5580
cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 5640
ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 5700
tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 5760
catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 5820
gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 5880
gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 5940
tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 6000
catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 6060
aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 6120
attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 6180
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac              6228



<210> 38
<211> 6080
<212> DNA
<213> Artificial Sequence

<220> 
<223> Viral vector CaLCuV A Plus FLC Consensus
      

<400> 38
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60
attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120
gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180
caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240
ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300
cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360
agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420
cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480
caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540
gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600
taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 660
gccccccctc gaggttaacc caagcagcgg ccttatcaaa tttatggatc atacctgcac 720
gaggtgaata gttagcattg cgggacactt tagaggtccc cgccatagaa cgccacgggg 780
catcccgctt aggcatttcg aagtaaagca taaattgaaa tcacatgtct ttatataggc 840
ccacagcgca ctcagttagc cttaatagct aaccttgtca ggcggcatgt gattggttag 900
atttcattta atgcgtggtc ccacgcaaga caccaaaaaa atcgcggcca tccggtaata 960
ttattgcgga tggccgcttt tgcccctggt gttccccaga ggcttttagt atatattgga 1020
caccaagaca ccaaaaacaa taggttttag agagagctct cctggcgtcc tcccctggag 1080
tcctaaatat tatttacaaa aatgccacga aaccctaaat cgtttcgttt agcagcccga 1140
aatatattct taacatatcc ccagtgcgac atacccaaag atgaagctct tcagatgctt 1200
caaaccctgt cgtggtcagt cgtcaaaccc acatacatca gagtcgcaag agaggaacat 1260
tcagacgggt tcccccattt acactgtctc atccaactat caggaaagtc gaacatcaag 1320
gatgctagat ttttcgacat cactcacccc agaaggtctg ccaattttca cccaaacatt 1380
caggcagcca aagacaccaa tgccgtcaag aattacatca ccaaagatgg tgattattgt 1440
gaatccgggc agtacaaggt gtctgggggt acaaaggcaa ataaagacga cgtctaccac 1500
aacgccgtca atgcgggatg tgtggaagag gctctcgcaa ttataagggc tggagatcca 1560
aagacgttca ttgttagtta tcataatgtt agagctaaca tagagcgact ctttactaag 1620
gctccggaac catgggctcc tccgtttcaa ctctcctcct ttactaacgt cccggacgag 1680
atgagttcat gggcagatga ctattttggt cggagtgccg ctgcgcgggc ggaaagacct 1740
attagtatca tagttgaagg tgattcacga accggcaaga ccatgtgggc gcgtgcttta 1800
ggaccacata attatttgag tgggcacctc gactttaatt caaaggtctt ttcaaataat 1860
gcggagtata acgtcattga tgacatagct ccgcattatc taaagctaaa gcactggaaa 1920
gagcttattg gggcccaaag ggactggcaa tcaaactgta agtacggcaa gccagttcaa 1980
attaaaggtg gcataccctc aatcgtgctg tgcaatccag gagaggggag cagttatata 2040
agtttcctca acaaagagga aaatgcatca ctaagagcgt ggactaccaa aaatgcaaaa 2100
ttcatcactc ttgaagcccc cctctatcaa agcacagcac aagattgcta aaaggagagc 2160
tgtacgccga agacgcattg atttgaactg cgggtgttct atcttcctcc acatcaattg 2220
cgcagataat ggattcacgc accggggaga gcatcactgt agctcaggca gagaattccg 2280
tttttatctg ggaggttcca aatcccctat atttcaggat acaacgcgta gaggacccgt 2340
tgtacaccag aaccaggatc taccacatcc aagtccggtt caaccacaac ctacggaaag 2400
cattggatct ccgcaaagcc tacttcaact tccaagtctg gacgacttcg atgagagctt 2460
ctgggccgac atatttaagt agattcaaat gccttgtaat gtctcattta gataacctag 2520
gtgttattgg tattaatcat gtaattagag ctgttcgttt cgcaacggac agatcttatg 2580
taactcatgt tcacgagaat catgtaataa atttcaaaat ttattaattt gttatcgaat 2640
cgtaaaaata gatccgaatt tttaatgtcg catacacagg attagaggca tgagtacatg 2700
ccatatacaa taacagagcg ttctccgtat gattctcgta tttccctgct tcttgatggt 2760
tgtacacaac gtagttattg accttccaaa aacgcttcac caacgcctgc tcgttactcg 2820
catactgccc accggttacc tttgcgtaaa acttgtgcat gacttgataa cgatcacgaa 2880
gatcgttctt cacagtagca gtgacggtat cgattggttc tcttctttca gcaaattctc 2940
cttttctttg aggctatcac aagcttcaac attagttctg tcttcctagc tctagttacg 3000
gagagggcct gcagcccaac ccaagcagcg gccttatcaa atttatggat catacctgca 3060
cgaggtgaat agttagcatt gcgggacact ttagaggtcc ccgccataga acgccacggg 3120
gcatcccgct taggcatttc gaagtaaagc ataaattgaa atcacatgtc tttatatagg 3180
cccacagcgc actcagttag ccttaatagc taaccttgtc aggcggcatg tgattggtta 3240
gatttcattt aatgcgtggt cccacgcaag acaccaaaaa aatcgcggcc atccggtaat 3300
attattgcgg atggccgctt ttgcccctgg tgttccccag aggcttttag tatatattgg 3360
acaccaagac accaaaaaca ataggtttta gagagagctc tcctggcgtc ctcccctgga 3420
gtcctaaata ttatttacaa aaatgccacg aaaccctaaa tcgtttcgtt tagcagcccg 3480
aaatatattc ttaacatatc cccagtgcga catacccaaa gatgaagctc ttcagatgct 3540
tcaaaccctg tcgtggtcag tcgtcaaacc cacatacatc agagtcgcaa gagaggaaca 3600
ttcagacggg ttcccccatt tacactgtct catccaacta tcaggaaagt cgaacatcaa 3660
ggatgctaga tttttcgaca tcactcaccc cagaaggtct gccaattttc acccaaacat 3720
tcaggcagcc aaagacacca atgccgtcaa gaattacatc accaaagatg gtgattattg 3780
tgaatccggg cagtacaagg tgtctggggg tacaaaggca aataaagacg acgtggggga 3840
tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg 3900
agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 3960
tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 4020
ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 4080
aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 4140
tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 4200
gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 4260
cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 4320
gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 4380
aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 4440
ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 4500
cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 4560
ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 4620
cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 4680
agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 4740
gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 4800
gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4860
tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 4920
agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 4980
agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 5040
atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 5100
cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 5160
actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 5220
aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 5280
cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 5340
ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 5400
cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 5460
ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 5520
cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 5580
ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 5640
tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 5700
ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 5760
aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 5820
gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 5880
gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 5940
ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 6000
catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 6060
atttccccga aaagtgccac                                             6080



<210> 39
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer PDSR

<400> 39
tcccaagttc tccaaataag ttc                                         23

<210> 40
<211> 95
<212> DNA
<213> Brassica napus

<400> 40
gccctctccg taacaagagc taggaagaca gaactaatgt tgaagcttgt cgagaacctt 60
aaagaaaagg agaagttgct ggaagaggag aacca                            95

<210> 41
<211> 95
<212> DNA
<213> Brassica napus

<400> 41
gccctctccg taactagagc taggaagaca gaactaatgt tgaagcttgt tgatagcctc 60
aaagaaaagg agaaattgct gaaagaagag aacca                            95

<210> 42
<211> 95
<212> DNA
<213> Brassica napus

<400> 42
gccctctctc taactagagc taggaagaca gaactaatgt tgaagcttgt tgatagcctc 60
aaagaaaagg agaagctgct gaaagaagag aatca                            95

<210> 43
<211> 95
<212> DNA
<213> Brassica napus

<400> 43
gccctctccg taactagagc taggaagaca gaactattgt tgaagcttgt tgatagcctc 60
aaagaaaagg agaaattgct gaaagaagag aacca                            95

<210> 44
<211> 95
<212> DNA
<213> Brassica napus

<400> 44
gccctctctg taactagagc tcggaaggca gaactaatgt taaagcttgt tgaaagtctc 60
aaagaaaagg agaatctgct gaaagaagag aacca                            95

<210> 45
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer PDSF

<400> 45
actgtcctgc aggatatacc aaggccagag ctaga                            35

<210> 46
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer PDSR

<400> 46
ctatgcatcg attcccaagt tctccaaata agttc                            35
