
                                SEQUENCE LISTING

<110> CENTOCOR ORTHO BIOTECH INC.

<120> USE OF THE ANTI-APOPTOTIC GENE HUMAN-MURINE DOUBLE
       MINUTE-2 (MDM2) IN THE DEVELOPMENT OF PRODUCTION CELL
       LINES EXPRESSING THERAPEUTIC PROTEINS

<130> CEN5223PCT

<140> TO BE ASSIGNED      
<141> 2009-06-12  

<150> 61/061235            
<151> 2008-06-13  

<160> 15

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1476
<212> DNA
<213> Homo sapiens

<400> 1
atgtgcaata ccaacatgtc tgtacctact gatggtgctg taaccacctc acagattcca 60
gcttcggaac aagagaccct ggttagacca aagccattgc ttttgaagtt attaaagtct 120
gttggtgcac aaaaagacac ttatactatg aaagaggttc ttttttatct tggccagtat 180
attatgacta aacgattata tgatgagaag caacaacata ttgtatattg ttcaaatgat 240
cttctaggag atttgtttgg cgtgccaagc ttctctgtga aagagcacag gaaaatatat 300
accatgatct acaggaactt ggtagtagtc aatcagcagg aatcatcgga ctcaggtaca 360
tctgtgagtg agaacaggtg tcaccttgaa ggtgggagtg atcaaaagga ccttgtacaa 420
gagcttcagg aagagaaacc ttcatcttca catttggttt ctagaccatc tacctcatct 480
agaaggagag caattagtga gacagaagaa aattcagatg aattatctgg tgaacgacaa 540
agaaaacgcc acaaatctga tagtatttcc ctttcctttg atgaaagcct ggctctgtgt 600
gtaataaggg agatatgttg tgaaagaagc agtagcagtg aatctacagg gacgccatcg 660
aatccggatc ttgatgctgg tgtaagtgaa cattcaggtg attggttgga tcaggattca 720
gtttcagatc agtttagtgt agaatttgaa gttgaatctc tcgactcaga agattatagc 780
cttagtgaag aaggacaaga actctcagat gaagatgatg aggtatatca agttactgtg 840
tatcaggcag gggagagtga tacagattca tttgaagaag atcctgaaat ttccttagct 900
gactattgga aatgcacttc atgcaatgaa atgaatcccc cccttccatc acattgcaac 960
agatgttggg cccttcgtga gaattggctt cctgaagata aagggaaaga taaaggggaa 1020
atctctgaga aagccaaact ggaaaactca acacaagctg aagagggctt tgatgttcct 1080
gattgtaaaa aaactatagt gaatgattcc agagagtcat gtgttgagga aaatgatgat 1140
aaaattacac aagcttcaca atcacaagaa agtgaagact attctcagcc atcaacttct 1200
agtagcatta tttatagcag ccaagaagat gtgaaagagt ttgaaaggga agaaacccaa 1260
gacaaagaag agagtgtgga atctagtttg ccccttaatg ccattgaacc ttgtgtgatt 1320
tgtcaaggtc gacctaaaaa tggttgcatt gtccatggca aaacaggaca tcttatggcc 1380
tgctttacat gtgcaaagaa gctaaagaaa aggaataagc cctgcccagt atgtagacaa 1440
ccaattcaaa tgattgtgct aacttatttc ccctag                           1476

<210> 2
<211> 491
<212> PRT
<213> Homo sapiens

<400> 2
Met Cys Asn Thr Asn Met Ser Val Pro Thr Asp Gly Ala Val Thr Thr
 1               5                  10                  15      
Ser Gln Ile Pro Ala Ser Glu Gln Glu Thr Leu Val Arg Pro Lys Pro
            20                  25                  30          
Leu Leu Leu Lys Leu Leu Lys Ser Val Gly Ala Gln Lys Asp Thr Tyr
        35                  40                  45              
Thr Met Lys Glu Val Leu Phe Tyr Leu Gly Gln Tyr Ile Met Thr Lys
    50                  55                  60                  
Arg Leu Tyr Asp Glu Lys Gln Gln His Ile Val Tyr Cys Ser Asn Asp
65                  70                  75                  80  
Leu Leu Gly Asp Leu Phe Gly Val Pro Ser Phe Ser Val Lys Glu His
                85                  90                  95      
Arg Lys Ile Tyr Thr Met Ile Tyr Arg Asn Leu Val Val Val Asn Gln
            100                 105                 110         
Gln Glu Ser Ser Asp Ser Gly Thr Ser Val Ser Glu Asn Arg Cys His
        115                 120                 125             
Leu Glu Gly Gly Ser Asp Gln Lys Asp Leu Val Gln Glu Leu Gln Glu
    130                 135                 140                 
Glu Lys Pro Ser Ser Ser His Leu Val Ser Arg Pro Ser Thr Ser Ser
145                 150                 155                 160 
Arg Arg Arg Ala Ile Ser Glu Thr Glu Glu Asn Ser Asp Glu Leu Ser
                165                 170                 175     
Gly Glu Arg Gln Arg Lys Arg His Lys Ser Asp Ser Ile Ser Leu Ser
            180                 185                 190         
Phe Asp Glu Ser Leu Ala Leu Cys Val Ile Arg Glu Ile Cys Cys Glu
        195                 200                 205             
Arg Ser Ser Ser Ser Glu Ser Thr Gly Thr Pro Ser Asn Pro Asp Leu
    210                 215                 220                 
Asp Ala Gly Val Ser Glu His Ser Gly Asp Trp Leu Asp Gln Asp Ser
225                 230                 235                 240 
Val Ser Asp Gln Phe Ser Val Glu Phe Glu Val Glu Ser Leu Asp Ser
                245                 250                 255     
Glu Asp Tyr Ser Leu Ser Glu Glu Gly Gln Glu Leu Ser Asp Glu Asp
            260                 265                 270         
Asp Glu Val Tyr Gln Val Thr Val Tyr Gln Ala Gly Glu Ser Asp Thr
        275                 280                 285             
Asp Ser Phe Glu Glu Asp Pro Glu Ile Ser Leu Ala Asp Tyr Trp Lys
    290                 295                 300                 
Cys Thr Ser Cys Asn Glu Met Asn Pro Pro Leu Pro Ser His Cys Asn
305                 310                 315                 320 
Arg Cys Trp Ala Leu Arg Glu Asn Trp Leu Pro Glu Asp Lys Gly Lys
                325                 330                 335     
Asp Lys Gly Glu Ile Ser Glu Lys Ala Lys Leu Glu Asn Ser Thr Gln
            340                 345                 350         
Ala Glu Glu Gly Phe Asp Val Pro Asp Cys Lys Lys Thr Ile Val Asn
        355                 360                 365             
Asp Ser Arg Glu Ser Cys Val Glu Glu Asn Asp Asp Lys Ile Thr Gln
    370                 375                 380                 

Ala Ser Gln Ser Gln Glu Ser Glu Asp Tyr Ser Gln Pro Ser Thr Ser
385                 390                 395                 400 
Ser Ser Ile Ile Tyr Ser Ser Gln Glu Asp Val Lys Glu Phe Glu Arg
                405                 410                 415     
Glu Glu Thr Gln Asp Lys Glu Glu Ser Val Glu Ser Ser Leu Pro Leu
            420                 425                 430         
Asn Ala Ile Glu Pro Cys Val Ile Cys Gln Gly Arg Pro Lys Asn Gly
        435                 440                 445             
Cys Ile Val His Gly Lys Thr Gly His Leu Met Ala Cys Phe Thr Cys
    450                 455                 460                 
Ala Lys Lys Leu Lys Lys Arg Asn Lys Pro Cys Pro Val Cys Arg Gln
465                 470                 475                 480 
Pro Ile Gln Met Ile Val Leu Thr Tyr Phe Pro
                485                 490     


<210> 3
<211> 1292
<212> DNA
<213> Artificial Sequence

<220> 
<223> Engineered mutant of human MDM2 gene with A899C substitution

<400> 3
atgactaaac gattatatga tgagaagcaa caacatattg tatattgttc aaatgatctt 60
ctaggagatt tgtttggcgt gccaagcttc tctgtgaaag agcacaggaa aatatatacc 120
atgatctaca ggaacttggt agtagtcaat cagcaggaat catcggactc aggtacatct 180
gtgagtgaga acaggtgtca ccttgaaggt gggagtgatc aaaaggacct tgtacaagag 240
cttcaggaag agaaaccttc atcttcacat ttggtttcta gaccatctac ctcatctaga 300
aggagagcaa ttagtgagac agaagaaaat tcagatgaat tatctggtga acgacaaaga 360
aaacgccaca aatctgatag tatttccctt tcctttgatg aaagcctggc tctgtgtgta 420
ataagggaga tatgttgtga aagaagcagt agcagtgaat ctacagggac gccatcgaat 480
ccggatcttg atgctggtgt aagtgaacat tcaggtgatt ggttggatca ggattcagtt 540
tcagatcagt ttagtgtaga atttgaagtt gaatctctcg actcagaaga ttatagcctt 600
agtgaagaag gacaagaact ctcagatgaa gatgatgagg tatatcaagt tactgtgtat 660
caggcagggg agagtgatac agattcattt gaagaagatc ctgaaatttc cttagctgac 720
tattggaaat gcacttcatg caatgaaatg aatccccccc ttccatcaca ttgcaacaga 780
tgttgggccc ttcgtgagaa ttggcttcct gaagataaag ggaaagataa aggggaaatc 840
tctgagaaag ccaaactgga aaactcaaca caagctgaag agggctttga tgtgccggct 900
tgtaaaaaac tatagtgaat gattccagag agtcatgtgt tgaggaaaat gatgataaaa 960
ttacacaagc ttcacaatca caagaaagtg aagactattc tcagccatca acttctagta 1020
gcattattta tagcagccaa gaagatgtga aagagtttga aagggaagaa acccaagaca 1080
aagaagagag tgtggaatct agtttgcccc ttaatgccat tgaaccttgt gtgatttgtc 1140
aaggtcgacc taaaaatggt tgcattgtcc atggcaaaac aggacatctt atggcctgct 1200
ttacatgtgc aaagaagcta aagaaaagga ataagccctg cccagtatgt agacaaccaa 1260
ttcaaatgat tgtgctaact tatttcccct ag                               1292

<210> 4
<211> 430
<212> PRT
<213> Artificial Sequence

<220> 

<223> Engineered mutant of human MDM2 protein with Asp300AIa
	substitution 
      

<400> 4
Met Thr Lys Arg Leu Tyr Asp Glu Lys Gln Gln His Ile Val Tyr Cys
 1               5                  10                  15      
Ser Asn Asp Leu Leu Gly Asp Leu Phe Gly Val Pro Ser Phe Ser Val
            20                  25                  30          
Lys Glu His Arg Lys Ile Tyr Thr Met Ile Tyr Arg Asn Leu Val Val
        35                  40                  45              
Val Asn Gln Gln Glu Ser Ser Asp Ser Gly Thr Ser Val Ser Glu Asn
    50                  55                  60                  
Arg Cys His Leu Glu Gly Gly Ser Asp Gln Lys Asp Leu Val Gln Glu
65                  70                  75                  80  
Leu Gln Glu Glu Lys Pro Ser Ser Ser His Leu Val Ser Arg Pro Ser
                85                  90                  95      
Thr Ser Ser Arg Arg Arg Ala Ile Ser Glu Thr Glu Glu Asn Ser Asp
            100                 105                 110         
Glu Leu Ser Gly Glu Arg Gln Arg Lys Arg His Lys Ser Asp Ser Ile
        115                 120                 125             
Ser Leu Ser Phe Asp Glu Ser Leu Ala Leu Cys Val Ile Arg Glu Ile
    130                 135                 140                 
Cys Cys Glu Arg Ser Ser Ser Ser Glu Ser Thr Gly Thr Pro Ser Asn
145                 150                 155                 160 
Pro Asp Leu Asp Ala Gly Val Ser Glu His Ser Gly Asp Trp Leu Asp
                165                 170                 175     
Gln Asp Ser Val Ser Asp Gln Phe Ser Val Glu Phe Glu Val Glu Ser
            180                 185                 190         
Leu Asp Ser Glu Asp Tyr Ser Leu Ser Glu Glu Gly Gln Glu Leu Ser
        195                 200                 205             
Asp Glu Asp Asp Glu Val Tyr Gln Val Thr Val Tyr Gln Ala Gly Glu
    210                 215                 220                 
Ser Asp Thr Asp Ser Phe Glu Glu Asp Pro Glu Ile Ser Leu Ala Asp
225                 230                 235                 240 
Tyr Trp Lys Cys Thr Ser Cys Asn Glu Met Asn Pro Pro Leu Pro Ser
                245                 250                 255     
His Cys Asn Arg Cys Trp Ala Leu Arg Glu Asn Trp Leu Pro Glu Asp
            260                 265                 270         
Lys Gly Lys Asp Lys Gly Glu Ile Ser Glu Lys Ala Lys Leu Glu Asn
        275                 280                 285             
Ser Thr Gln Ala Glu Glu Gly Phe Asp Val Pro Ala Cys Lys Lys Thr
    290                 295                 300                 
Ile Val Asn Asp Ser Arg Glu Ser Cys Val Glu Glu Asn Asp Asp Lys
305                 310                 315                 320 
Ile Thr Gln Ala Ser Gln Ser Gln Glu Ser Glu Asp Tyr Ser Gln Pro
                325                 330                 335     
Ser Thr Ser Ser Ser Ile Ile Tyr Ser Ser Gln Glu Asp Val Lys Glu
            340                 345                 350         
Phe Glu Arg Glu Glu Thr Gln Asp Lys Glu Glu Ser Val Glu Ser Ser
        355                 360                 365             
Leu Pro Leu Asn Ala Ile Glu Pro Cys Val Ile Cys Gln Gly Arg Pro
    370                 375                 380                 
Lys Asn Gly Cys Ile Val His Gly Lys Thr Gly His Leu Met Ala Cys
385                 390                 395                 400 
Phe Thr Cys Ala Lys Lys Leu Lys Lys Arg Asn Lys Pro Cys Pro Val
                405                 410                 415     
Cys Arg Gln Pro Ile Gln Met Ile Val Leu Thr Tyr Phe Pro
            420                 425                 430 


<210> 5
<211> 660
<212> DNA
<213> Homo sapiens

<400> 5
atgtcgtccc acctagtcga gccgccgccg cccctgcaca acaacaacaa caactgcgag 60
gaaaatgagc agtctctgcc cccgccggcc ggcctcaaca gttcctgggt ggagctaccc 120
atgaacagca gcaatggcaa tgataatggc aatgggaaaa atggggggct ggaacacgta 180
ccatcctcat cctccatcca caatggagac atggagaaga ttcttttgga tgcacaacat 240
gaatcaggac agagtagttc cagaggcagt tctcactgtg acagcccttc gccacaagaa 300
gatgggcaga tcatgtttga tgtggaaatg cacaccagca gggaccatag ctctcagtca 360
gaagaagaag ttgtagaagg agagaaggaa gtcgaggctt tgaagaaaag tgcggactgg 420
gtatcagact ggtccagtag acccgaaaac attccaccca aggagttcca cttcagacac 480
cctaaacgtt ctgtgtcttt aagcatgagg aaaagtggag ccatgaagaa agggggtatt 540
ttctccgcag aatttctgaa ggtgttcatt ccatctctct tcctttctca tgttttggct 600
ttggggctag gcatctatat tggaaagcga ctgagcacac cctctgccag cacctactga 660


<210> 6
<211> 219
<212> PRT
<213> Homo sapiens

<400> 6
Met Ser Ser His Leu Val Glu Pro Pro Pro Pro Leu His Asn Asn Asn
 1               5                  10                  15      
Asn Asn Cys Glu Glu Asn Glu Gln Ser Leu Pro Pro Pro Ala Gly Leu
            20                  25                  30          
Asn Ser Ser Trp Val Glu Leu Pro Met Asn Ser Ser Asn Gly Asn Asp
        35                  40                  45              
Asn Gly Asn Gly Lys Asn Gly Gly Leu Glu His Val Pro Ser Ser Ser
    50                  55                  60                  
Ser Ile His Asn Gly Asp Met Glu Lys Ile Leu Leu Asp Ala Gln His
65                  70                  75                  80  
Glu Ser Gly Gln Ser Ser Ser Arg Gly Ser Ser His Cys Asp Ser Pro
                85                  90                  95      
Ser Pro Gln Glu Asp Gly Gln Ile Met Phe Asp Val Glu Met His Thr
            100                 105                 110         
Ser Arg Asp His Ser Ser Gln Ser Glu Glu Glu Val Val Glu Gly Glu
        115                 120                 125             
Lys Glu Val Glu Ala Leu Lys Lys Ser Ala Asp Trp Val Ser Asp Trp
    130                 135                 140                 
Ser Ser Arg Pro Glu Asn Ile Pro Pro Lys Glu Phe His Phe Arg His
145                 150                 155                 160 

Pro Lys Arg Ser Val Ser Leu Ser Met Arg Lys Ser Gly Ala Met Lys
                165                 170                 175     
Lys Gly Gly Ile Phe Ser Ala Glu Phe Leu Lys Val Phe Ile Pro Ser
            180                 185                 190         
Leu Phe Leu Ser His Val Leu Ala Leu Gly Leu Gly Ile Tyr Ile Gly
        195                 200                 205             
Lys Arg Leu Ser Thr Pro Ser Ala Ser Thr Tyr
    210                 215                 


<210> 7
<211> 1089
<212> DNA
<213> Homo sapiens

<400> 7
atgcaggcgg agcgaggagc tcggggaggc cgtgggcggc ggccaggccg cggccggcct 60
ggcggagatc gccacagcga gcggcccgga gccgcagcgg cggtagccag aggcggcggc 120
ggaggcggcg gcggggacgg aggcggacgc cggggccgtg gccgtggccg gggcttccgc 180
ggcgctcgcg gaggccgagg aggaggaggc gccccgcgag gcagccgccg ggagccggga 240
ggctggggcg caggggccag cgcgccggtt gaagatgaca gcgatgcaga gacctatgga 300
gaagagaatg atgaacaggg aaattattct aaaagaaaga ttgtctctaa ctgggatcga 360
tatcaagata ttgaaaaaga ggtcaataat gaaagtggag agtcacagag gggaacagat 420
ttcagtgtcc tccttagctc tgcaggggac tcattctcac agttccggtt tgctgaggag 480
aaagaatggg atagtgaagc ttcttgtcca aaacagaatt cagcatttta tgtggatagt 540
gagttattgg ttcgagccct tcaagagctg cctctctgcc tccgactcaa cgttgctgcc 600
gaactggtcc agggtacagt tcctttagag gttcctcagg tgaaaccaaa gagaactgat 660
gatggcaagg gattagggat gcagttaaag gggcccttgg ggcctggagg aagggggccc 720
atctttgagc tgaaatctgt ggctgctggc tgccctgtgt tgctgggcaa agacaaccca 780
agcccgggtc cttcaaggga ttctcagaaa cccacttccc cactgcagtc agcaggagac 840
catttggaag aagaactaga tctgttgctt aatttagatg cacctataaa agagggagat 900
aacatcttac cagatcagac gtctcaggac ctgaaatcca aggaagatgg ggaggtggtc 960
caagaggaag aagtttgtgc aaaaccatct gtgactgaag aaaaaaacat ggaacctgag 1020
caaccaagta cctccaaaaa tgttaccgag gaagagctgg aagactggtt ggacagcatg 1080
atttcctaa                                                         1089

<210> 8
<211> 362
<212> PRT
<213> Homo sapiens

<400> 8
Met Gln Ala Glu Arg Gly Ala Arg Gly Gly Arg Gly Arg Arg Pro Gly
 1               5                  10                  15      
Arg Gly Arg Pro Gly Gly Asp Arg His Ser Glu Arg Pro Gly Ala Ala
            20                  25                  30          
Ala Ala Val Ala Arg Gly Gly Gly Gly Gly Gly Gly Gly Asp Gly Gly
        35                  40                  45              
Gly Arg Arg Gly Arg Gly Arg Gly Arg Gly Phe Arg Gly Ala Arg Gly
    50                  55                  60                  
Gly Arg Gly Gly Gly Gly Ala Pro Arg Gly Ser Arg Arg Glu Pro Gly
65                  70                  75                  80  
Gly Trp Gly Ala Gly Ala Ser Ala Pro Val Glu Asp Asp Ser Asp Ala
                85                  90                  95      
Glu Thr Tyr Gly Glu Glu Asn Asp Glu Gln Gly Asn Tyr Ser Lys Arg
            100                 105                 110         
Lys Ile Val Ser Asn Trp Asp Arg Tyr Gln Asp Ile Glu Lys Glu Val
        115                 120                 125             
Asn Asn Glu Ser Gly Glu Ser Gln Arg Gly Thr Asp Phe Ser Val Leu
    130                 135                 140                 
Leu Ser Ser Ala Gly Asp Ser Phe Ser Gln Phe Arg Phe Ala Glu Glu
145                 150                 155                 160 
Lys Glu Trp Asp Ser Glu Ala Ser Cys Pro Lys Gln Asn Ser Ala Phe
                165                 170                 175     
Tyr Val Asp Ser Glu Leu Leu Val Arg Ala Leu Gln Glu Leu Pro Leu
            180                 185                 190         
Cys Leu Arg Leu Asn Val Ala Ala Glu Leu Val Gln Gly Thr Val Pro
        195                 200                 205             
Leu Glu Val Pro Gln Val Lys Pro Lys Arg Thr Asp Asp Gly Lys Gly
    210                 215                 220                 
Leu Gly Met Gln Leu Lys Gly Pro Leu Gly Pro Gly Gly Arg Gly Pro
225                 230                 235                 240 
Ile Phe Glu Leu Lys Ser Val Ala Ala Gly Cys Pro Val Leu Leu Gly
                245                 250                 255     
Lys Asp Asn Pro Ser Pro Gly Pro Ser Arg Asp Ser Gln Lys Pro Thr
            260                 265                 270         
Ser Pro Leu Gln Ser Ala Gly Asp His Leu Glu Glu Glu Leu Asp Leu
        275                 280                 285             
Leu Leu Asn Leu Asp Ala Pro Ile Lys Glu Gly Asp Asn Ile Leu Pro
    290                 295                 300                 
Asp Gln Thr Ser Gln Asp Leu Lys Ser Lys Glu Asp Gly Glu Val Val
305                 310                 315                 320 
Gln Glu Glu Glu Val Cys Ala Lys Pro Ser Val Thr Glu Glu Lys Asn
                325                 330                 335     
Met Glu Pro Glu Gln Pro Ser Thr Ser Lys Asn Val Thr Glu Glu Glu
            340                 345                 350         
Leu Glu Asp Trp Leu Asp Ser Met Ile Ser
        355                 360         


<210> 9
<211> 513
<212> DNA
<213> Homo sapiens

<400> 9
atgtctcaga gcaaccggga gctggtggtt gactttctct cctacaagct ttcccagaaa 60
ggatacagct ggagtcagtt tagtgatgtg gaagagaaca ggactgaggc cccagaaggg 120
actgaatcgg agatggagac ccccagtgcc atcaatggca acccatcctg gcacctggca 180
gacagccccg cggtgaatgg agccactggc cacagcagca gtttggatgc ccgggaggtg 240
atccccatgg cagcagtaaa gcaagcgctg agggaggcag gcgacgagtt tgaactgcgg 300
taccggcggg cattcagtga cctgacatcc cagctccaca tcaccccagg gacagcatat 360
cagagctttg aacaggatac ttttgtggaa ctctatggga acaatgcagc agccgagagc 420
cgaaagggcc aggaacgctt caaccgctgg ttcctgacgg gcatgactgt ggccggcgtg 480
gttctgctgg gctcactctt cagtcggaaa tga                              513

<210> 10
<211> 233
<212> PRT
<213> Homo sapiens

<400> 10
Met Ser Gln Ser Asn Arg Glu Leu Val Val Asp Phe Leu Ser Tyr Lys
 1               5                  10                  15      
Leu Ser Gln Lys Gly Tyr Ser Trp Ser Gln Phe Ser Asp Val Glu Glu
            20                  25                  30          
Asn Arg Thr Glu Ala Pro Glu Gly Thr Glu Ser Glu Met Glu Thr Pro
        35                  40                  45              
Ser Ala Ile Asn Gly Asn Pro Ser Trp His Leu Ala Asp Ser Pro Ala
    50                  55                  60                  
Val Asn Gly Ala Thr Gly His Ser Ser Ser Leu Asp Ala Arg Glu Val
65                  70                  75                  80  
Ile Pro Met Ala Ala Val Lys Gln Ala Leu Arg Glu Ala Gly Asp Glu
                85                  90                  95      
Phe Glu Leu Arg Tyr Arg Arg Ala Phe Ser Asp Leu Thr Ser Gln Leu
            100                 105                 110         
His Ile Thr Pro Gly Thr Ala Tyr Gln Ser Phe Glu Gln Val Val Asn
        115                 120                 125             
Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile Val Ala Phe Phe
    130                 135                 140                 
Ser Phe Gly Gly Ala Leu Cys Val Glu Ser Val Asp Lys Glu Met Gln
145                 150                 155                 160 
Val Leu Val Ser Arg Ile Ala Ala Trp Met Ala Thr Tyr Leu Asn Asp
                165                 170                 175     
His Leu Glu Pro Trp Ile Gln Glu Asn Gly Gly Trp Asp Thr Phe Val
            180                 185                 190         
Glu Leu Tyr Gly Asn Asn Ala Ala Ala Glu Ser Arg Lys Gly Gln Glu
        195                 200                 205             
Arg Phe Asn Arg Trp Phe Leu Thr Gly Met Thr Val Ala Gly Val Val
    210                 215                 220                 
Leu Leu Gly Ser Leu Phe Ser Arg Lys
225                 230             


<210> 11
<211> 573
<212> DNA
<213> Homo sapiens

<400> 11
atggcgcacg ctgggagaac agggtacgat aaccgggaga tagtgatgaa gtacatccat 60
tataagctgt cgcagagggg ctacgagtgg gatgccgcgg ggcctgcgct cagcccggtg 120
ccacctgtgg tccacctgac cctccgccag gccggcgacg acttctcccg ccgctaccgc 180
cgcgacttcg ccgagatgtc cagccagctg cacctgacgc ccttcaccgc gcggggacgc 240
tttgccacgg tggtggagga gctcttcagg gacggggtga actgggggag gattgtggcc 300
ttctttgagt tcggtggggt catgtgtgtg gagagcgtca accgggagat gtcgcccctg 360
gtggacaaca tcgccctgtg gatgactgag tacctgaacc ggcacctgca cacctggatc 420
caggataacg gaggctggga tgcctttgtg gaactgtacg gccccagcat gcggcctctg 480
tttgatttct cctggctgtc tctgaagact ctgctcagtt tggccctggt gggagcttgc 540
atcaccctgg gtgcctatct gagccacaag tga                              573

<210> 12
<211> 190
<212> PRT
<213> Homo sapiens

<400> 12
Met Ala His Ala Gly Arg Thr Gly Tyr Asp Asn Arg Glu Ile Val Met
 1               5                  10                  15      
Lys Tyr Ile His Tyr Lys Leu Ser Gln Arg Gly Tyr Glu Trp Asp Ala
            20                  25                  30          
Ala Gly Pro Ala Leu Ser Pro Val Pro Pro Val Val His Leu Thr Leu
        35                  40                  45              
Arg Gln Ala Gly Asp Asp Phe Ser Arg Arg Tyr Arg Arg Asp Phe Ala
    50                  55                  60                  
Glu Met Ser Ser Gln Leu His Leu Thr Pro Phe Thr Ala Arg Gly Arg
65                  70                  75                  80  
Phe Ala Thr Val Val Glu Glu Leu Phe Arg Asp Gly Val Asn Trp Gly
                85                  90                  95      
Arg Ile Val Ala Phe Phe Glu Phe Gly Gly Val Met Cys Val Glu Ser
            100                 105                 110         
Val Asn Arg Glu Met Ser Pro Leu Val Asp Asn Ile Ala Leu Trp Met
        115                 120                 125             
Thr Glu Tyr Leu Asn Arg His Leu His Thr Trp Ile Gln Asp Asn Gly
    130                 135                 140                 
Gly Trp Asp Ala Phe Val Glu Leu Tyr Gly Pro Ser Met Arg Pro Leu
145                 150                 155                 160 
Phe Asp Phe Ser Trp Leu Ser Leu Lys Thr Leu Leu Ser Leu Ala Leu
                165                 170                 175     
Val Gly Ala Cys Ile Thr Leu Gly Ala Tyr Leu Ser His Lys
            180                 185                 190 


<210> 13
<211> 1356
<212> DNA
<213> Homo sapiens

<400> 13
atgactttta acagttttga aggatctaaa acttgtgtac ctgcagacat caataaggaa 60
gaagaatttg tagaagagtt taatagatta aaaacttttg ctaattttcc aagtggtagt 120
cctgtttcag catcaacact ggcacgagca gggtttcttt atactggtga aggagatacc 180
gtgcggtgct ttagttgtca tgcagctgta gatagatggc aatatggaga ctcagcagtt 240
ggaagacaca ggaaagtatc cccaaattgc agatttatca acggctttta tcttgaaaat 300
agtgccacgc agtctacaaa ttctggtatc cagaatggtc agtacaaagt tgaaaactat 360
ctgggaagca gagatcattt tgccttagac aggccatctg agacacatgc agactatctt 420
ttgagaactg ggcaggttgt agatatatca gacaccatat acccgaggaa ccctgccatg 480
tatagtgaag aagctagatt aaagtccttt cagaactggc cagactatgc tcacctaacc 540
ccaagagagt tagcaagtgc tggactctac tacacaggta ttggtgacca agtgcagtgc 600
ttttgttgtg gtggaaaact gaaaaattgg gaaccttgtg atcgtgcctg gtcagaacac 660
aggcgacact ttcctaattg cttctttgtt ttgggccgga atcttaatat tcgaagtgaa 720
tctgatgctg tgagttctga taggaatttc ccaaattcaa caaatcttcc aagaaatcca 780
tccatggcag attatgaagc acggatcttt acttttggga catggatata ctcagttaac 840
aaggagcagc ttgcaagagc tggattttat gctttaggtg aaggtgataa agtaaagtgc 900
tttcactgtg gaggagggct aactgattgg aagcccagtg aagacccttg ggaacaacat 960
gctaaatggt atccagggtg caaatatctg ttagaacaga agggacaaga atatataaac 1020
aatattcatt taactcattc acttgaggag tgtctggtaa gaactactga gaaaacacca 1080
tcactaacta gaagaattga tgataccatc ttccaaaatc ctatggtaca agaagctata 1140
cgaatggggt tcagtttcaa ggacattaag aaaataatgg aggaaaaaat tcagatatct 1200
gggagcaact ataaatcact tgaggttctg gttgcagatc tagtgaatgc tcagaaagac 1260
agtatgccag atgagtcaag tcagacttca ttacagaaag agattagtac tgaagagcag 1320
ctaaggcgcc tgcaagagga gaagcttatc gattga                           1356

<210> 14
<211> 451
<212> PRT
<213> Homo sapiens

<400> 14
Met Thr Phe Asn Ser Phe Glu Gly Ser Lys Thr Cys Val Pro Ala Asp
 1               5                  10                  15      
Ile Asn Lys Glu Glu Glu Phe Val Glu Glu Phe Asn Arg Leu Lys Thr
            20                  25                  30          
Phe Ala Asn Phe Pro Ser Gly Ser Pro Val Ser Ala Ser Thr Leu Ala
        35                  40                  45              
Arg Ala Gly Phe Leu Tyr Thr Gly Glu Gly Asp Thr Val Arg Cys Phe
    50                  55                  60                  
Ser Cys His Ala Ala Val Asp Arg Trp Gln Tyr Gly Asp Ser Ala Val
65                  70                  75                  80  
Gly Arg His Arg Lys Val Ser Pro Asn Cys Arg Phe Ile Asn Gly Phe
                85                  90                  95      
Tyr Leu Glu Asn Ser Ala Thr Gln Ser Thr Asn Ser Gly Ile Gln Asn
            100                 105                 110         
Gly Gln Tyr Lys Val Glu Asn Tyr Leu Gly Ser Arg Asp His Phe Ala
        115                 120                 125             
Leu Asp Arg Pro Ser Glu Thr His Ala Asp Tyr Leu Leu Arg Thr Gly
    130                 135                 140                 
Gln Val Val Asp Ile Ser Asp Thr Ile Tyr Pro Arg Asn Pro Ala Met
145                 150                 155                 160 
Tyr Ser Glu Glu Ala Arg Leu Lys Ser Phe Gln Asn Trp Pro Asp Tyr
                165                 170                 175     
Ala His Leu Thr Pro Arg Glu Leu Ala Ser Ala Gly Leu Tyr Tyr Thr
            180                 185                 190         
Gly Ile Gly Asp Gln Val Gln Cys Phe Cys Cys Gly Gly Lys Leu Lys
        195                 200                 205             
Asn Trp Glu Pro Cys Asp Arg Ala Trp Ser Glu His Arg Arg His Phe
    210                 215                 220                 
Pro Asn Cys Phe Phe Val Leu Gly Arg Asn Leu Asn Ile Arg Ser Glu
225                 230                 235                 240 
Ser Asp Ala Val Ser Ser Asp Arg Asn Phe Pro Asn Ser Thr Asn Leu
                245                 250                 255     
Pro Arg Asn Pro Ser Met Ala Asp Tyr Glu Ala Arg Ile Phe Thr Phe
            260                 265                 270         
Gly Thr Trp Ile Tyr Ser Val Asn Lys Glu Gln Leu Ala Arg Ala Gly
        275                 280                 285             
Phe Tyr Ala Leu Gly Glu Gly Asp Lys Val Lys Cys Phe His Cys Gly
    290                 295                 300                 
Gly Gly Leu Thr Asp Trp Lys Pro Ser Glu Asp Pro Trp Glu Gln His
305                 310                 315                 320 

Ala Lys Trp Tyr Pro Gly Cys Lys Tyr Leu Leu Glu Gln Lys Gly Gln
                325                 330                 335     
Glu Tyr Ile Asn Asn Ile His Leu Thr His Ser Leu Glu Glu Cys Leu
            340                 345                 350         
Val Arg Thr Thr Glu Lys Thr Pro Ser Leu Thr Arg Arg Ile Asp Asp
        355                 360                 365             
Thr Ile Phe Gln Asn Pro Met Val Gln Glu Ala Ile Arg Met Gly Phe
    370                 375                 380                 
Ser Phe Lys Asp Ile Lys Lys Ile Met Glu Glu Lys Ile Gln Ile Ser
385                 390                 395                 400 
Gly Ser Asn Tyr Lys Ser Leu Glu Val Leu Val Ala Asp Leu Val Asn
                405                 410                 415     
Ala Gln Lys Asp Ser Met Pro Asp Glu Ser Ser Gln Thr Ser Leu Gln
            420                 425                 430         
Lys Glu Ile Ser Thr Glu Glu Gln Leu Arg Arg Leu Gln Glu Glu Lys
        435                 440                 445             
Leu Ile Asp
    450     


<210> 15
<211> 45
<212> DNA
<213> Homo sapiens

<400> 15
gctgaagagg gctttgatgt gccggcttgt aaaaaaacta tagtg                 45
