                         SEQUENCE LISTING

<110>  Life Technologies Corporation
       Xi, Larry L.
 
<120>  MUTATED AND CHEMICALLY MODIFIED THERMALLY STABLE DNA POLYMERASES

<130>  7052WO

<140>  To be assigned
<141>  2009-06-18

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  832
<212>  PRT
<213>  Thermus aquaticus


<220>
<221>  MUTAGEN
<222>  (11)..(11)
<223>  Lysine substituted with Arginine

<220>
<221>  DOMAIN
<222>  (12)..(264)
<223>  5'-3' exonuclease

<220>
<221>  MUTAGEN
<222>  (31)..(31)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (82)..(82)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (131)..(131)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (225)..(225)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (240)..(240)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (260)..(260)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (292)..(292)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (340)..(340)
<223>  Lysine substituted with Arginine

<220>
<221>  DOMAIN
<222>  (451)..(830)
<223>  Pol A DNA Polymerase

<220>
<221>  MUTAGEN
<222>  (505)..(505)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (508)..(508)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (511)..(511)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (531)..(531)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (540)..(540)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (542)..(542)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (663)..(663)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (804)..(804)
<223>  Lysine substituted with Arginine

<220>
<221>  MUTAGEN
<222>  (831)..(831)
<223>  Lysine substituted with Arginine

<300>
<308>  GenBank / AAA27507.1
<309>  1989-06-23
<313>  (1)..(832)

<400>  1

Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  


Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  


Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      


Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         


Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             


Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 


Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 


Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     


Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         


Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             


Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 


Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 


Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         


Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             


Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg 
                725                 730                 735     


Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         


<210>  2
<211>  8244
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFC64


<220>
<221>  source
<222>  (1)..(8244)
<223>  pFC64

<220>
<221>  gene
<222>  (1291)..(3789)
<223>  Taq DNA Polymerase

<400>  2
ctctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt       60

ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc      120

attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg      180

tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat      240

gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca      300

gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat      360

taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct      420

ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg      480

gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga      540

ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg      600

cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgtgc      660

gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg      720

actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa      780

ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg      840

gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct      900

gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta      960

gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc     1020

tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgga aatcataaaa     1080

aattatttgc tttgtgagcg gataacaatt ataatagatt caattgaggc ctcgaccacc     1140

gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc     1200

atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga     1260

tataccatgg cgatatcccg ggagctccat atgcgcggta tgctgccact gtttgaaccg     1320

aaaggccgcg tactgctggt ggatggccat catctggcgt atcgcacctt tcatgcgctg     1380

aagggtctga ccacgtctcg tggtgagccg gtgcaggcgg tttatggctt cgctaagagc     1440

ctgctgaaag cactgaaaga agatggcgac gcagtaattg tggtattcga tgctaaggcg     1500

ccatctttcc gtcacgaagc gtatggtggc tacaaagcgg gtcgcgcccc gacgccagaa     1560

gatttccctc gtcaactggc actgattaag gagctggtgg atctgctggg cctggcgcgt     1620

ctggaggtac caggttacga ggcggatgac gtcctggcca gcctggcgaa gaaagctgaa     1680

aaggagggct atgaagtccg catcctgacg gccgataaag atctgtacca actgctgtcc     1740

gaccgtatcc acgtgctgca ccctgaaggt tatctgatca ccccggcttg gctgtgggaa     1800

aagtatggtc tgcgtccaga tcagtgggct gactatcgcg cgctgaccgg cgacgaatcc     1860

gacaacctgc cgggcgtcaa aggtatcggc gaaaagaccg cgcgcaaact gctggaagaa     1920

tggggcagcc tggaagccct gctgaagaat ctggaccgcc tgaaaccggc tattcgcgaa     1980

aagatcctgg ctcacatgga tgacctgaaa ctgagctggg acctggcgaa ggttcgcacc     2040

gacctgccgc tggaggttga cttcgctaag cgtcgcgagc cggatcgcga gcgcctgcgc     2100

gcgttcctgg aacgtctgga atttggttcc ctgctgcatg aatttggtct gctggagtcc     2160

ccgaaagcac tggaagaagc gccatggcct ccgccggaag gcgcgttcgt tggcttcgtt     2220

ctgtctcgta aagagcctat gtgggcggac ctgctggctc tggcggccgc acgcggcggc     2280

cgtgtgcatc gtgctccaga gccgtataaa gcgctgcgcg atctgaaaga agctcgcggt     2340

ctgctggcaa aagacctgag cgtcctggct ctgcgcgaag gcctgggtct gccgccgggc     2400

gacgacccga tgctgctggc ttacctgctg gacccgtcca acacgacgcc agaaggcgtt     2460

gctcgccgtt acggtggtga atggaccgaa gaagcgggtg aacgtgccgc gctgagcgag     2520

cgtctgttcg caaacctgtg gggtcgtctg gaaggtgagg aacgcctgct gtggctgtac     2580

cgtgaggttg agcgtccact gtctgccgta ctggctcaca tggaagcgac cggtgtgcgt     2640

ctggacgtag cgtacctgcg tgcgctgtct ctggaagttg ctgaggagat tgcgcgtctg     2700

gaggcggaag ttttccgtct ggcaggtcat ccattcaacc tgaactcccg cgatcagctg     2760

gaacgcgttc tgttcgacga gctgggtctg ccagccatcg gcaaaactga gaaaaccggc     2820

aaacgcagca cttccgccgc cgtgctggaa gctctgcgcg aggcacaccc gatcgttgag     2880

aaaattctgc aataccgtga gctgaccaaa ctgaagtcga cctacatcga cccactgccg     2940

gacctgattc atccacgtac cggccgcctg cacactcgtt tcaaccagac cgcaactgct     3000

accggccgtc tgagcagctc cgaccctaac ctgcaaaaca ttccggttcg taccccgctg     3060

ggtcaacgca tccgtcgcgc ctttatcgcc gaggaaggct ggctgctggt tgcgctggac     3120

tacagccaaa tcgaactgcg cgtcctggcg cacctgtccg gcgatgaaaa cctgattcgt     3180

gtcttccagg agggtcgtga catccacacc gagaccgctt cttggatgtt cggtgtcccg     3240

cgtgaagcag tggacccgct gatgcgccgt gcagcaaaga ccatcaattt cggcgttctg     3300

tacggtatgt ctgcacatcg tctgtctcag gaactggcca tcccatacga agaagcccag     3360

gcgttcatcg aacgctattt tcagtctttc ccgaaagttc gtgcctggat cgaaaagacg     3420

ctggaagagg gccgccgtcg cggttatgtt gagacgctgt tcggtcgtcg tcgttacgtt     3480

ccggacctgg aagcccgcgt taagagcgtc cgtgaagcgg ctgaacgtat ggcatttaat     3540

atgccggtcc agggcactgc cgctgacctg atgaagctgg ccatggtgaa actgttcccg     3600

cgtctggaag aaatgggtgc tcgtatgctg ctccaggtcc atgacgaact ggtcctggaa     3660

gctccgaagg aacgtgctga agccgttgcg cgtctggcga aagaggtcat ggaaggcgtt     3720

tacccgctgg cagtccctct ggaagttgaa gtaggcatcg gtgaggactg gctgtctgct     3780

aaagaataac tcgagctgca ggtcgacggt accggttcga agcttgcggc cgcacagctg     3840

tatacacgtg caagccagcc agaactcgcc ccggaagacc ccgaggatct cgagcaccac     3900

catcaccatc accatcacta agtgattaac ctcaggtgca ggctgcctat cagaaggtgg     3960

tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcgatct ttttccctct     4020

gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg     4080

aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca     4140

tatgggaggg caaatcattt aaaacatcag aatgagtttt tggtttagag tttggcaaca     4200

tatgcccata tgtaactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt     4260

ttgctgaaag catgcggagg aaattctcct tgaagtttcc ctggtgttca aagtaaagga     4320

gtttgcacca gacgcacctc tgttcactgg tccggcgtat taaaacacga tacattgtta     4380

ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac     4440

gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa     4500

atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa     4560

taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa     4620

tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc     4680

gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc     4740

ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa     4800

tagagcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc     4860

gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg     4920

cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat     4980

tatttctgat tgcgggcgtt tttgggcggg tttcaatcta actgtgcccg attttaattc     5040

agacaacacg ttagaaagcg atggtgcagg cggtggtaac atttcagacg gcaaatctac     5100

taatggcggc ggtggtggag ctgatgataa atctaccatc ggtggaggcg caggcggggc     5160

tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca gacggcggtt taggctcaaa     5220

tgtctcttta ggcaacacag tcggcacctc aactattgta ctggtttcgg gcgccgtttt     5280

tggtttgacc ggtctgagac gagtgcgatt tttttcgttt ctaatagctt ccaacaattg     5340

ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc ggcattggtg gagcgggcgg     5400

caattcagac atcgatggtg gtggtggtgg tggaggcgct ggaatgttag gcacgggaga     5460

aggtggtggc ggcggtgccg ccggtataat ttgttctggt ttagtttgtt cgcgcacgat     5520

tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag     5580

cgcttggggt ggtggcaatt caatattata attggaatac aaatcgtaaa aatctgctat     5640

aagcattgta atttcgctat cgtttaccgt gccgatattt aacaaccgct caatgtaagc     5700

aattgtattg taaagagatt gtctcaagct cggaacgctg cgctcggtcg ttcggctgcg     5760

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     5820

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     5880

gttgctggcg tttttccata ggctccgccc cctgacgagc atcacaaaaa tcgacgctca     5940

agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc     6000

tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc     6060

ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag     6120

gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc     6180

ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca     6240

gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg     6300

aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg     6360

aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct     6420

ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa     6480

gaagatcctt tgttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     6540

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt     6600

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt     6660

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc     6720

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa     6780

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg     6840

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt     6900

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc     6960

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt     7020

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg     7080

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac     7140

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc     7200

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt     7260

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg     7320

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag     7380

catttatcag ggttattgtc tcatgtccgc gcgtttcctg catcttttaa tcaaatccca     7440

agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt     7500

tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata     7560

aatgtcaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta     7620

ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca     7680

aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg     7740

tcgtcttctt cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag     7800

ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata     7860

tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca     7920

acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa     7980

tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct     8040

tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg     8100

tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt     8160

tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg     8220

gaaatgtcta tcaatatata gttg                                            8244


<210>  3
<211>  2499
<212>  DNA
<213>  Thermus aquaticus

<300>
<308>  GenBank / J04639
<309>  1989-06-23
<313>  (1)..(2499)

<400>  3
atgaggggga tgctgcccct ctttgagccc aagggccggg tcctcctggt ggacggccac       60

cacctggcct accgcacctt ccacgccctg aagggcctca ccaccagccg gggggagccg      120

gtgcaggcgg tctacggctt cgccaagagc ctcctcaagg ccctcaagga ggacggggac      180

gcggtgatcg tggtctttga cgccaaggcc ccctccttcc gccacgaggc ctacgggggg      240

tacaaggcgg gccgggcccc cacgccggag gactttcccc ggcaactcgc cctcatcaag      300

gagctggtgg acctcctggg gctggcgcgc ctcgaggtcc cgggctacga ggcggacgac      360

gtcctggcca gcctggccaa gaaggcggaa aaggagggct acgaggtccg catcctcacc      420

gccgacaaag acctttacca gctcctttcc gaccgcatcc acgtcctcca ccccgagggg      480

tacctcatca ccccggcctg gctttgggaa aagtacggcc tgaggcccga ccagtgggcc      540

gactaccggg ccctgaccgg ggacgagtcc gacaaccttc ccggggtcaa gggcatcggg      600

gagaagacgg cgaggaagct tctggaggag tgggggagcc tggaagccct cctcaagaac      660

ctggaccggc tgaagcccgc catccgggag aagatcctgg cccacatgga cgatctgaag      720

ctctcctggg acctggccaa ggtgcgcacc gacctgcccc tggaggtgga cttcgccaaa      780

aggcgggagc ccgaccggga gaggcttagg gcctttctgg agaggcttga gtttggcagc      840

ctcctccacg agttcggcct tctggaaagc cccaaggccc tggaggaggc cccctggccc      900

ccgccggaag gggccttcgt gggctttgtg ctttcccgca aggagcccat gtgggccgat      960

cttctggccc tggccgccgc cagggggggc cgggtccacc gggcccccga gccttataaa     1020

gccctcaggg acctgaagga ggcgcggggg cttctcgcca aagacctgag cgttctggcc     1080

ctgagggaag gccttggcct cccgcccggc gacgacccca tgctcctcgc ctacctcctg     1140

gacccttcca acaccacccc cgagggggtg gcccggcgct acggcgggga gtggacggag     1200

gaggcggggg agcgggccgc cctttccgag aggctcttcg ccaacctgtg ggggaggctt     1260

gagggggagg agaggctcct ttggctttac cgggaggtgg agaggcccct ttccgctgtc     1320

ctggcccaca tggaggccac gggggtgcgc ctggacgtgg cctatctcag ggccttgtcc     1380

ctggaggtgg ccgaggagat cgcccgcctc gaggccgagg tcttccgcct ggccggccac     1440

cccttcaacc tcaactcccg ggaccagctg gaaagggtcc tctttgacga gctagggctt     1500

cccgccatcg gcaagacgga gaagaccggc aagcgctcca ccagcgccgc cgtcctggag     1560

gccctccgcg aggcccaccc catcgtggag aagatcctgc agtaccggga gctcaccaag     1620

ctgaagagca cctacattga ccccttgccg gacctcatcc accccaggac gggccgcctc     1680

cacacccgct tcaaccagac ggccacggcc acgggcaggc taagtagctc cgatcccaac     1740

ctccagaaca tccccgtccg caccccgctt gggcagagga tccgccgggc cttcatcgcc     1800

gaggaggggt ggctattggt ggccctggac tatagccaga tagagctcag ggtgctggcc     1860

cacctctccg gcgacgagaa cctgatccgg gtcttccagg aggggcggga catccacacg     1920

gagaccgcca gctggatgtt cggcgtcccc cgggaggccg tggaccccct gatgcgccgg     1980

gcggccaaga ccatcaactt cggggtcctc tacggcatgt cggcccaccg cctctcccag     2040

gagctagcca tcccttacga ggaggcccag gccttcattg agcgctactt tcagagcttc     2100

cccaaggtgc gggcctggat tgagaagacc ctggaggagg gcaggaggcg ggggtacgtg     2160

gagaccctct tcggccgccg ccgctacgtg ccagacctag aggcccgggt gaagagcgtg     2220

cgggaggcgg ccgagcgcat ggccttcaac atgcccgtcc agggcaccgc cgccgacctc     2280

atgaagctgg ctatggtgaa gctcttcccc aggctggagg aaatgggggc caggatgctc     2340

cttcaggtcc acgacgagct ggtcctcgag gccccaaaag agagggcgga ggccgtggcc     2400

cggctggcca aggaggtcat ggagggggtg tatcccctgg ccgtgcccct ggaggtggag     2460

gtggggatag gggaggactg gctctccgcc aaggagtga                            2499


<210>  4
<211>  27
<212>  DNA
<213>  Primer

<400>  4
ctgacccgtc tgcgttcgac ctacatc                                           27


<210>  5
<211>  27
<212>  DNA
<213>  Primer

<400>  5
ggtcgaacgc agacgggtca gctcacg                                           27


<210>  6
<211>  23
<212>  DNA
<213>  Primer

<400>  6
tacaatcaaa ggagatatac cat                                               23


<210>  7
<211>  20
<212>  DNA
<213>  Primer

<400>  7
cacctgaggt taatcactta                                                   20


<210>  8
<211>  30
<212>  DNA
<213>  Primer 505Rf

<400>  8
ccagccatcg gccgtactga gaaaaccggc                                        30


<210>  9
<211>  30
<212>  DNA
<213>  Primer 505Rr

<400>  9
ggttttctca gtacggccga tggctggcag                                        30


<210>  10
<211>  54
<212>  DNA
<213>  Polsub14 substrate

<400>  10
atcatcatat catcaactgg ccgtcgtttt acatatgtaa aacgacggcc agtt             54


<210>  11
<211>  19
<212>  DNA
<213>  GapdhFwd primer

<400>  11
gaaggtgaag gtcggagtc                                                    19


<210>  12
<211>  20
<212>  DNA
<213>  gapdh Rev primer

<400>  12
gaagatggtg atgggatttc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Probe

<400>  13
caagcttccc gttctcagcc                                                   20


<210>  14
<211>  834
<212>  PRT
<213>  Thermus caldophilus

<300>
<308>  GenBank / AAB81398.1
<309>  1996-06-28
<313>  (1)..(834)

<400>  14

Met Glu Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Asn Pro Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Asp Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Gln Lys Tyr Gly Leu Lys 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Leu Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Asn Val Arg Glu Lys Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Arg Leu Ser Leu Glu Leu Ser Arg Val Arg Thr Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Leu Ala Gln Gly Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 


Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 


Ala Glu Leu Lys Ala Leu Ala Ala Cys Arg Asp Gly Arg Val His Arg 
                325                 330                 335     


Ala Ala Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         


Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Ser Arg Glu Gly Leu Asp 
        355                 360                 365             


Leu Val Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 


Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 


Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ser Glu Arg Leu His Arg 
                405                 410                 415     


Asn Leu Leu Lys Arg Leu Gln Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         


His Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             


Thr Gly Val Arg Leu Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu 
    450                 455                 460                 


Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 


Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     


Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         


Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             


Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 


Asn Thr Tyr Val Asp Pro Leu Pro Ser Leu Val His Pro Asn Thr Gly 
545                 550                 555                 560 


Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     


Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu 
            580                 585                 590         


Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             


Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu 
    610                 615                 620                 


Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Lys Asp Ile 
625                 630                 635                 640 


His Thr Gln Thr Ala Ser Trp Met Phe Gly Val Pro Pro Glu Ala Val 
                645                 650                 655     


Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu 
            660                 665                 670         


Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
        675                 680                 685             


Glu Glu Ala Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 


Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 


Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     


Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
            740                 745                 750         


Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             


Lys Leu Phe Pro Arg Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 


Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Gly Ala Glu Glu 
785                 790                 795                 800 


Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     


Val Pro Leu Glu Val Glu Val Gly Met Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         


Lys Gly 
        


<210>  15
<211>  834
<212>  PRT
<213>  Thermus thermophilus

<300>
<308>  GenBank / BAA06033.1
<309>  1994-03-11
<313>  (1)..(834)

<400>  15

Met Glu Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Lys Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Arg 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Leu Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Asn Val Arg Glu Lys Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Arg Leu Ser Leu Glu Leu Ser Arg Val Arg Thr Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Leu Ala Gln Gly Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 


Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 


Ala Glu Leu Lys Ala Leu Ala Ala Cys Arg Asp Gly Arg Val His Arg 
                325                 330                 335     


Ala Ala Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         


Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Ser Arg Glu Gly Leu Asp 
        355                 360                 365             


Leu Val Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 


Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 


Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ser Glu Arg Leu His Arg 
                405                 410                 415     


Asn Leu Leu Lys Arg Leu Glu Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         


His Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             


Thr Gly Val Arg Arg Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu 
    450                 455                 460                 


Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 


Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     


Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         


Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             


Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 


Asn Thr Tyr Val Asp Pro Leu Pro Ser Leu Val His Pro Arg Thr Gly 
545                 550                 555                 560 


Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     


Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu 
            580                 585                 590         


Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             


Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu 
    610                 615                 620                 


Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Lys Asp Ile 
625                 630                 635                 640 


His Thr Gln Thr Ala Ser Trp Met Phe Gly Val Pro Pro Glu Ala Val 
                645                 650                 655     


Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu 
            660                 665                 670         


Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
        675                 680                 685             


Glu Glu Ala Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 


Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 


Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     


Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
            740                 745                 750         


Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             


Lys Leu Phe Pro Arg Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 


Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Arg Ala Glu Glu 
785                 790                 795                 800 


Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     


Val Pro Leu Glu Val Glu Val Gly Met Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         


Lys Gly 
        


<210>  16
<211>  928
<212>  PRT
<213>  Escherichia coli

<300>
<308>  GenBank / AAA24402.1
<309>  1993-04-26
<313>  (1)..(928)

<400>  16

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Gln Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Met Ala His Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  17
<211>  704
<212>  PRT
<213>  enterobacteria phage T7

<300>
<308>  GenBank / NP_041982.1
<309>  1993-11-12
<313>  (1)..(704)

<400>  17

Met Ile Val Ser Asp Ile Glu Ala Asn Ala Leu Leu Glu Ser Val Thr 
1               5                   10                  15      


Lys Phe His Cys Gly Val Ile Tyr Asp Tyr Ser Thr Ala Glu Tyr Val 
            20                  25                  30          


Ser Tyr Arg Pro Ser Asp Phe Gly Ala Tyr Leu Asp Ala Leu Glu Ala 
        35                  40                  45              


Glu Val Ala Arg Gly Gly Leu Ile Val Phe His Asn Gly His Lys Tyr 
    50                  55                  60                  


Asp Val Pro Ala Leu Thr Lys Leu Ala Lys Leu Gln Leu Asn Arg Glu 
65                  70                  75                  80  


Phe His Leu Pro Arg Glu Asn Cys Ile Asp Thr Leu Val Leu Ser Arg 
                85                  90                  95      


Leu Ile His Ser Asn Leu Lys Asp Thr Asp Met Gly Leu Leu Arg Ser 
            100                 105                 110         


Gly Lys Leu Pro Gly Lys Arg Phe Gly Ser His Ala Leu Glu Ala Trp 
        115                 120                 125             


Gly Tyr Arg Leu Gly Glu Met Lys Gly Glu Tyr Lys Asp Asp Phe Lys 
    130                 135                 140                 


Arg Met Leu Glu Glu Gln Gly Glu Glu Tyr Val Asp Gly Met Glu Trp 
145                 150                 155                 160 


Trp Asn Phe Asn Glu Glu Met Met Asp Tyr Asn Val Gln Asp Val Val 
                165                 170                 175     


Val Thr Lys Ala Leu Leu Glu Lys Leu Leu Ser Asp Lys His Tyr Phe 
            180                 185                 190         


Pro Pro Glu Ile Asp Phe Thr Asp Val Gly Tyr Thr Thr Phe Trp Ser 
        195                 200                 205             


Glu Ser Leu Glu Ala Val Asp Ile Glu His Arg Ala Ala Trp Leu Leu 
    210                 215                 220                 


Ala Lys Gln Glu Arg Asn Gly Phe Pro Phe Asp Thr Lys Ala Ile Glu 
225                 230                 235                 240 


Glu Leu Tyr Val Glu Leu Ala Ala Arg Arg Ser Glu Leu Leu Arg Lys 
                245                 250                 255     


Leu Thr Glu Thr Phe Gly Ser Trp Tyr Gln Pro Lys Gly Gly Thr Glu 
            260                 265                 270         


Met Phe Cys His Pro Arg Thr Gly Lys Pro Leu Pro Lys Tyr Pro Arg 
        275                 280                 285             


Ile Lys Thr Pro Lys Val Gly Gly Ile Phe Lys Lys Pro Lys Asn Lys 
    290                 295                 300                 


Ala Gln Arg Glu Gly Arg Glu Pro Cys Glu Leu Asp Thr Arg Glu Tyr 
305                 310                 315                 320 


Val Ala Gly Ala Pro Tyr Thr Pro Val Glu His Val Val Phe Asn Pro 
                325                 330                 335     


Ser Ser Arg Asp His Ile Gln Lys Lys Leu Gln Glu Ala Gly Trp Val 
            340                 345                 350         


Pro Thr Lys Tyr Thr Asp Lys Gly Ala Pro Val Val Asp Asp Glu Val 
        355                 360                 365             


Leu Glu Gly Val Arg Val Asp Asp Pro Glu Lys Gln Ala Ala Ile Asp 
    370                 375                 380                 


Leu Ile Lys Glu Tyr Leu Met Ile Gln Lys Arg Ile Gly Gln Ser Ala 
385                 390                 395                 400 


Glu Gly Asp Lys Ala Trp Leu Arg Tyr Val Ala Glu Asp Gly Lys Ile 
                405                 410                 415     


His Gly Ser Val Asn Pro Asn Gly Ala Val Thr Gly Arg Ala Thr His 
            420                 425                 430         


Ala Phe Pro Asn Leu Ala Gln Ile Pro Gly Val Arg Ser Pro Tyr Gly 
        435                 440                 445             


Glu Gln Cys Arg Ala Ala Phe Gly Ala Glu His His Leu Asp Gly Ile 
    450                 455                 460                 


Thr Gly Lys Pro Trp Val Gln Ala Gly Ile Asp Ala Ser Gly Leu Glu 
465                 470                 475                 480 


Leu Arg Cys Leu Ala His Phe Met Ala Arg Phe Asp Asn Gly Glu Tyr 
                485                 490                 495     


Ala His Glu Ile Leu Asn Gly Asp Ile His Thr Lys Asn Gln Ile Ala 
            500                 505                 510         


Ala Glu Leu Pro Thr Arg Asp Asn Ala Lys Thr Phe Ile Tyr Gly Phe 
        515                 520                 525             


Leu Tyr Gly Ala Gly Asp Glu Lys Ile Gly Gln Ile Val Gly Ala Gly 
    530                 535                 540                 


Lys Glu Arg Gly Lys Glu Leu Lys Lys Lys Phe Leu Glu Asn Thr Pro 
545                 550                 555                 560 


Ala Ile Ala Ala Leu Arg Glu Ser Ile Gln Gln Thr Leu Val Glu Ser 
                565                 570                 575     


Ser Gln Trp Val Ala Gly Glu Gln Gln Val Lys Trp Lys Arg Arg Trp 
            580                 585                 590         


Ile Lys Gly Leu Asp Gly Arg Lys Val His Val Arg Ser Pro His Ala 
        595                 600                 605             


Ala Leu Asn Thr Leu Leu Gln Ser Ala Gly Ala Leu Ile Cys Lys Leu 
    610                 615                 620                 


Trp Ile Ile Lys Thr Glu Glu Met Leu Val Glu Lys Gly Leu Lys His 
625                 630                 635                 640 


Gly Trp Asp Gly Asp Phe Ala Tyr Met Ala Trp Val His Asp Glu Ile 
                645                 650                 655     


Gln Val Gly Cys Arg Thr Glu Glu Ile Ala Gln Val Val Ile Glu Thr 
            660                 665                 670         


Ala Gln Glu Ala Met Arg Trp Val Gly Asp His Trp Asn Phe Arg Cys 
        675                 680                 685             


Leu Leu Asp Thr Glu Gly Lys Met Gly Pro Asn Trp Ala Ile Cys His 
    690                 695                 700                 


<210>  18
<211>  833
<212>  PRT
<213>  Thermus filiformis

<300>
<308>  GenBank / AAR11876.1
<309>  2003-03-03
<313>  (1)..(833)

<400>  18

Met Thr Pro Leu Phe Asp Leu Glu Glu Pro Pro Lys Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Tyr Ala Leu Ser Leu 
            20                  25                  30          


Thr Thr Ser Arg Gly Glu Pro Val Gln Met Val Tyr Gly Phe Ala Arg 
        35                  40                  45              


Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Gln Ala Val Val Val Val 
    50                  55                  60                  


Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu Ala Tyr 
65                  70                  75                  80  


Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala 
                85                  90                  95      


Leu Val Lys Arg Leu Val Asp Leu Leu Gly Leu Val Arg Leu Glu Ala 
            100                 105                 110         


Pro Gly Tyr Glu Ala Asp Asp Val Leu Gly Thr Leu Ala Lys Lys Ala 
        115                 120                 125             


Glu Arg Glu Gly Met Glu Val Arg Ile Leu Thr Gly Asp Arg Asp Phe 
    130                 135                 140                 


Phe Gln Leu Leu Ser Glu Lys Val Ser Val Leu Leu Pro Asp Gly Thr 
145                 150                 155                 160 


Leu Val Thr Pro Lys Asp Val Gln Glu Lys Tyr Gly Val Pro Pro Glu 
                165                 170                 175     


Arg Trp Val Asp Phe Arg Ala Leu Thr Gly Asp Arg Ser Asp Asn Ile 
            180                 185                 190         


Pro Gly Val Ala Gly Ile Gly Glu Lys Thr Ala Leu Arg Leu Leu Ala 
        195                 200                 205             


Glu Trp Gly Ser Val Glu Asn Leu Leu Lys Asn Leu Asp Arg Val Lys 
    210                 215                 220                 


Pro Asp Ser Val Arg Arg Lys Ile Glu Ala His Leu Glu Asp Leu Arg 
225                 230                 235                 240 


Leu Ser Leu Asp Leu Ala Arg Ile Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Lys Ala Leu Arg Arg Arg Thr Pro Asp Leu Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Glu Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Gly Gly Glu Lys Pro Arg Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 


Glu Gly Ala Phe Val Gly Phe Leu Leu Ser Arg Lys Glu Pro Met Trp 
305                 310                 315                 320 


Ala Glu Leu Leu Ala Leu Ala Ala Ala Ala Glu Gly Arg Val His Arg 
                325                 330                 335     


Ala Thr Ser Pro Val Glu Ala Leu Ala Asp Leu Lys Glu Ala Arg Gly 
            340                 345                 350         


Phe Leu Ala Lys Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Val Ala 
        355                 360                 365             


Leu Asp Pro Thr Asp Asp Pro Leu Leu Val Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 


Ala Asn Thr Asn Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Phe 
385                 390                 395                 400 


Thr Glu Asp Ala Ala Glu Arg Ala Leu Leu Ser Glu Arg Leu Phe Gln 
                405                 410                 415     


Asn Leu Phe Pro Arg Leu Ser Glu Lys Leu Leu Trp Leu Tyr Gln Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Arg Val Leu Ala His Met Glu Ala Arg Gly 
        435                 440                 445             


Val Arg Leu Asp Val Pro Leu Leu Glu Ala Leu Ser Phe Glu Leu Glu 
    450                 455                 460                 


Lys Glu Met Glu Arg Leu Glu Gly Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Thr Pro Val Gly Arg Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ala Gln Gly Ala Leu Glu Ala Leu Arg Gly Ala His Pro Ile 
        515                 520                 525             


Val Glu Leu Ile Leu Gln Tyr Arg Glu Leu Ser Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Leu Asp Pro Leu Pro Arg Leu Val His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Lys Ala Phe Val Ala Glu Glu Gly Trp Leu Leu Leu Ala 
        595                 600                 605             


Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Lys Arg Val Phe Arg Glu Gly Lys Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ala Trp Met Phe Gly Leu Asp Pro Ala Leu Val Asp Pro 
                645                 650                 655     


Lys Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Gly Ile Asp Tyr Lys Glu 
        675                 680                 685             


Ala Glu Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Arg Thr Leu Glu Glu Gly Arg Thr Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Ala Ser Arg 
                725                 730                 735     


Val Arg Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Ile Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Lys Pro Leu Gly Ala His Leu Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Val Pro Glu Asp Arg Ala Glu Glu Ala Lys 
785                 790                 795                 800 


Ala Leu Val Lys Glu Val Met Glu Asn Thr Tyr Pro Leu Asp Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Val Gly Arg Asp Trp Leu Glu Ala Lys Gly 
            820                 825                 830         


Asp 
    


<210>  19
<211>  831
<212>  PRT
<213>  Thermus thermophilus

<300>
<308>  GenBank / CAA46900.1
<309>  1992-05-21
<313>  (1)..(831)

<400>  19

Met Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val 
1               5                   10                  15      


Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu 
            20                  25                  30          


Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys 
        35                  40                  45              


Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Val Val Val Val Val 
    50                  55                  60                  


Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu Ala Tyr 
65                  70                  75                  80  


Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala 
                85                  90                  95      


Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Val Arg Leu Glu Val 
            100                 105                 110         


Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys Arg Ala 
        115                 120                 125             


Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg Asp Leu 
    130                 135                 140                 


Tyr Gln Leu Leu Ser Glu Arg Ile Ala Ile Leu His Pro Glu Gly Tyr 
145                 150                 155                 160 


Leu Ile Thr Pro Ala Trp Leu Tyr Glu Lys Tyr Gly Leu Arg Pro Glu 
                165                 170                 175     


Gln Trp Val Asp Tyr Arg Ala Leu Ala Gly Asp Pro Ser Asp Asn Ile 
            180                 185                 190         


Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Gln Arg Leu Ile Arg 
        195                 200                 205             


Glu Trp Gly Ser Leu Glu Asn Leu Phe Gln His Leu Asp Gln Val Lys 
    210                 215                 220                 


Pro Ser Leu Arg Glu Lys Leu Gln Ala Gly Met Glu Ala Leu Ala Leu 
225                 230                 235                 240 


Ser Arg Lys Leu Ser Gln Val His Thr Asp Leu Pro Leu Glu Val Asp 
                245                 250                 255     


Phe Gly Arg Arg Arg Thr Pro Asn Leu Glu Gly Leu Arg Ala Phe Leu 
            260                 265                 270         


Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu Glu 
        275                 280                 285             


Gly Pro Lys Ala Ala Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala 
    290                 295                 300                 


Phe Leu Gly Phe Ser Phe Ser Arg Pro Glu Pro Met Trp Ala Glu Leu 
305                 310                 315                 320 


Leu Ala Leu Ala Gly Ala Trp Glu Gly Arg Leu His Arg Ala Gln Asp 
                325                 330                 335     


Pro Leu Arg Gly Leu Arg Asp Leu Lys Gly Val Arg Gly Ile Leu Ala 
            340                 345                 350         


Lys Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Leu Asp Leu Phe Pro 
        355                 360                 365             


Glu Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr 
    370                 375                 380                 


Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Asp 
385                 390                 395                 400 


Ala Gly Glu Arg Ala Leu Leu Ala Glu Arg Leu Phe Gln Thr Leu Lys 
                405                 410                 415     


Glu Arg Leu Lys Gly Glu Glu Arg Leu Leu Trp Leu Tyr Glu Glu Val 
            420                 425                 430         


Glu Lys Pro Leu Ser Arg Val Leu Ala Arg Met Glu Ala Thr Gly Val 
        435                 440                 445             


Arg Leu Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu Val Glu Ala 
    450                 455                 460                 


Glu Val Arg Gln Leu Glu Glu Glu Val Phe Arg Leu Ala Gly His Pro 
465                 470                 475                 480 


Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu 
                485                 490                 495     


Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser 
            500                 505                 510         


Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val 
        515                 520                 525             


Asp Arg Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Asn Thr Tyr 
    530                 535                 540                 


Ile Asp Pro Leu Pro Ala Leu Val His Pro Lys Thr Gly Arg Leu His 
545                 550                 555                 560 


Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser 
                565                 570                 575     


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg 
            580                 585                 590         


Ile Arg Arg Ala Phe Val Ala Glu Glu Gly Trp Val Leu Val Val Leu 
        595                 600                 605             


Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp 
    610                 615                 620                 


Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Gln 
625                 630                 635                 640 


Thr Ala Ser Trp Met Phe Gly Val Ser Pro Glu Gly Val Asp Pro Leu 
                645                 650                 655     


Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met 
            660                 665                 670         


Ser Ala His Arg Leu Ser Gly Glu Leu Ser Ile Pro Tyr Glu Glu Ala 
        675                 680                 685             


Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Tyr Pro Lys Val Arg Ala 
    690                 695                 700                 


Trp Ile Glu Gly Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu 
705                 710                 715                 720 


Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn Ala Arg Val 
                725                 730                 735     


Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val 
            740                 745                 750         


Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Arg Leu Phe 
        755                 760                 765             


Pro Arg Leu Gln Glu Leu Gly Ala Arg Met Leu Leu Gln Val His Asp 
    770                 775                 780                 


Glu Leu Val Leu Glu Ala Pro Lys Asp Arg Ala Glu Arg Val Ala Ala 
785                 790                 795                 800 


Leu Ala Lys Glu Val Met Glu Gly Val Trp Pro Leu Gln Val Pro Leu 
                805                 810                 815     


Glu Val Glu Val Gly Leu Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830     


<210>  20
<211>  876
<212>  PRT
<213>  Geobacillus stearothermophilus

<300>
<308>  GenBank / AAB52611.1
<309>  1995-08-04
<313>  (1)..(876)

<400>  20

Met Lys Lys Lys Leu Val Leu Ile Asp Gly Asn Ser Val Ala Tyr Arg 
1               5                   10                  15      


Ala Phe Phe Ala Leu Pro Leu Leu His Asn Asp Lys Gly Ile His Thr 
            20                  25                  30          


Asn Ala Val Tyr Gly Phe Thr Met Met Leu Asn Lys Ile Leu Ala Glu 
        35                  40                  45              


Glu Gln Pro Thr His Leu Leu Val Ala Phe Asp Ala Gly Lys Thr Thr 
    50                  55                  60                  


Phe Arg His Glu Thr Phe Gln Glu Tyr Lys Gly Gly Arg Gln Gln Thr 
65                  70                  75                  80  


Pro Pro Glu Leu Ser Glu Gln Phe Pro Leu Leu Arg Glu Leu Leu Lys 
                85                  90                  95      


Ala Tyr Arg Ile Pro Ala Tyr Glu Leu Asp His Tyr Glu Ala Asp Asp 
            100                 105                 110         


Ile Ile Gly Thr Leu Ala Ala Arg Ala Glu Gln Glu Gly Phe Glu Val 
        115                 120                 125             


Lys Ile Ile Ser Gly Asp Arg Asp Leu Thr Gln Leu Ala Ser Arg His 
    130                 135                 140                 


Val Thr Val Asp Ile Thr Lys Lys Gly Ile Thr Asp Ile Glu Pro Tyr 
145                 150                 155                 160 


Thr Pro Glu Thr Val Arg Glu Lys Tyr Gly Leu Thr Pro Glu Gln Ile 
                165                 170                 175     


Val Asp Leu Lys Gly Leu Met Gly Asp Lys Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Ile Gly Glu Lys Thr Ala Val Lys Leu Leu Lys Gln Phe 
        195                 200                 205             


Gly Thr Val Glu Asn Val Leu Ala Ser Ile Asp Glu Val Lys Gly Glu 
    210                 215                 220                 


Lys Leu Lys Glu Asn Leu Arg Gln His Arg Asp Leu Ala Leu Leu Ser 
225                 230                 235                 240 


Lys Gln Leu Ala Ser Ile Cys Arg Asp Ala Pro Val Glu Leu Ser Leu 
                245                 250                 255     


Asp Asp Ile Val Tyr Glu Gly Gln Asp Arg Glu Lys Val Ile Ala Leu 
            260                 265                 270         


Phe Lys Glu Leu Gly Phe Gln Ser Phe Leu Glu Lys Met Ala Ala Pro 
        275                 280                 285             


Ala Ala Glu Gly Glu Lys Pro Leu Glu Glu Met Glu Phe Ala Ile Val 
    290                 295                 300                 


Asp Val Ile Thr Glu Glu Met Leu Ala Asp Lys Ala Ala Leu Val Val 
305                 310                 315                 320 


Glu Val Met Glu Glu Asn Tyr His Asp Ala Pro Ile Val Gly Ile Ala 
                325                 330                 335     


Leu Val Asn Glu His Gly Arg Phe Phe Met Arg Pro Glu Thr Ala Leu 
            340                 345                 350         


Ala Asp Ser Gln Phe Leu Ala Trp Leu Ala Asp Glu Thr Lys Lys Lys 
        355                 360                 365             


Ser Met Phe Asp Ala Lys Arg Ala Val Val Ala Leu Lys Trp Lys Gly 
    370                 375                 380                 


Ile Glu Leu Arg Gly Val Ala Phe Asp Leu Leu Leu Ala Ala Tyr Leu 
385                 390                 395                 400 


Leu Asn Pro Ala Gln Asp Ala Gly Asp Ile Ala Ala Val Ala Lys Met 
                405                 410                 415     


Lys Gln Tyr Glu Ala Val Arg Ser Asp Glu Ala Val Tyr Gly Lys Gly 
            420                 425                 430         


Val Lys Arg Ser Leu Pro Asp Glu Gln Thr Leu Ala Glu His Leu Val 
        435                 440                 445             


Arg Lys Ala Ala Ala Ile Trp Ala Leu Glu Gln Pro Phe Met Asp Asp 
    450                 455                 460                 


Leu Arg Asn Asn Glu Gln Asp Gln Leu Leu Thr Lys Leu Glu Gln Pro 
465                 470                 475                 480 


Leu Ala Ala Ile Leu Ala Glu Met Glu Phe Thr Gly Val Asn Val Asp 
                485                 490                 495     


Thr Lys Arg Leu Glu Gln Met Gly Ser Glu Leu Ala Glu Gln Leu Arg 
            500                 505                 510         


Ala Ile Glu Gln Arg Ile Tyr Glu Leu Ala Gly Gln Glu Phe Asn Ile 
        515                 520                 525             


Asn Ser Pro Lys Gln Leu Gly Val Ile Leu Phe Glu Lys Leu Gln Leu 
    530                 535                 540                 


Pro Val Leu Lys Lys Thr Lys Thr Gly Tyr Ser Thr Ser Ala Asp Val 
545                 550                 555                 560 


Leu Glu Lys Leu Ala Pro His His Glu Ile Val Glu Asn Ile Leu His 
                565                 570                 575     


Tyr Arg Gln Leu Gly Lys Leu Gln Ser Thr Tyr Ile Glu Gly Leu Leu 
            580                 585                 590         


Lys Val Val Arg Pro Asp Thr Gly Lys Val His Thr Met Phe Asn Gln 
        595                 600                 605             


Ala Leu Thr Gln Thr Gly Arg Leu Ser Ser Ala Glu Pro Asn Leu Gln 
    610                 615                 620                 


Asn Ile Pro Ile Arg Leu Glu Glu Gly Arg Lys Ile Arg Gln Ala Phe 
625                 630                 635                 640 


Val Pro Ser Glu Pro Asp Trp Leu Ile Phe Ala Ala Asp Tyr Ser Gln 
                645                 650                 655     


Ile Glu Leu Arg Val Leu Ala His Ile Ala Asp Asp Asp Asn Leu Ile 
            660                 665                 670         


Glu Ala Phe Gln Arg Asp Leu Asp Ile His Thr Lys Thr Ala Met Asp 
        675                 680                 685             


Ile Phe His Val Ser Glu Glu Glu Val Thr Ala Asn Met Arg Arg Gln 
    690                 695                 700                 


Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly Ile Ser Asp Tyr Gly 
705                 710                 715                 720 


Leu Ala Gln Asn Leu Asn Ile Thr Arg Lys Glu Ala Ala Glu Phe Ile 
                725                 730                 735     


Glu Arg Tyr Phe Ala Ser Phe Pro Gly Val Lys Gln Tyr Met Glu Asn 
            740                 745                 750         


Ile Val Gln Glu Ala Lys Gln Lys Gly Tyr Val Thr Thr Leu Leu His 
        755                 760                 765             


Arg Arg Arg Tyr Leu Pro Asp Ile Thr Ser Arg Asn Phe Asn Val Arg 
    770                 775                 780                 


Ser Phe Ala Glu Arg Thr Ala Met Asn Thr Pro Ile Gln Gly Ser Ala 
785                 790                 795                 800 


Ala Asp Ile Ile Lys Lys Ala Met Ile Asp Leu Ala Ala Arg Leu Lys 
                805                 810                 815     


Glu Glu Gln Leu Gln Ala Arg Leu Leu Leu Gln Val His Asp Glu Leu 
            820                 825                 830         


Ile Leu Glu Ala Pro Lys Glu Glu Ile Glu Arg Leu Cys Glu Leu Val 
        835                 840                 845             


Pro Glu Val Met Glu Gln Ala Val Thr Leu Arg Val Pro Leu Lys Val 
    850                 855                 860                 


Asp Tyr His Tyr Gly Pro Thr Trp Tyr Asp Ala Lys 
865                 870                 875     


<210>  21
<211>  27
<212>  DNA
<213>  540Rfor primer

<400>  21
ctgacccgtc tgaagtcgac ctacatc                                           27


<210>  22
<211>  27
<212>  DNA
<213>  540Rrev primer

<400>  22
ggtcgacttc agacgggtca gctcacg                                           27


<210>  23
<211>  27
<212>  DNA
<213>  542Rfor primer

<400>  23
ctgaccaaac tgcgttcgac ctacatc                                           27


<210>  24
<211>  27
<212>  DNA
<213>  542Rrev primer

<400>  24
ggtcgaacgc agtttggtca gctcacg                                           27


