                         SEQUENCE LISTING

<110>  Edenspace Systems Corporation
       Alliance for Sustainable Energy, LLC
       Decker, Stephen R
       Selig, Michael J
       Brunecky, Roman
       Vinzant, Todd B
       Himmel, Michael E
       Lee, David A
       Blaylock, Michael J
 
<120>  Processing Cellulosic Biomass

<130>  2002647-0071

<150>  61/074,497
<151>  2008-06-20

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1167
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon optimized polynucleotide for E1 endo 1,4-beta-glucanase 
       from Acidothermus cellulolyticus

<400>  1
atgggcttcg ttctcttttc tcaactcccc tccttccttc tcgtttctac tcttcttctg       60

ttcctcgtaa tctcacattc atgtcgcgcc gcaggcggtg gttattggca tacttccggc      120

agagagatac ttgacgctaa caacgttccc gtacgcatcg ctggtattaa ttggtttggt      180

ttcgagacgt gcaattatgt cgttcacggt ctttggtctc gcgattaccg ttcaatgctg      240

gatcaaataa aatctctcgg ctacaataca attcgccttc cctactcgga tgatatcttg      300

aaaccaggta ctatgcccaa ctcaattaat ttttatcaaa tgaatcaaga ccttcaaggc      360

ctgacatccc ttcaagttat ggacaagata gttgcttacg caggacaaat aggacttagg      420

attattctcg acagacacag acccgactgc tctggccaaa gcgctctctg gtatacttca      480

tccgtcagtg aagctacctg gatctctgat cttcaagcac ttgcccaacg ttacaaagga      540

aaccctactg ttgttggttt cgatcttcac aacgaacctc acgatcccgc ctgttggggc      600

tgcggagacc catctattga ctggagattg gccgccgaac gtgctggcaa cgcagtgctg      660

tccgtaaatc ccaacctgct tatatttgtc gaaggcgtac aatcctataa tggtgactcc      720

tattggtggg gcggaaactt gcaaggcgca ggacagtatc cagttgtcct caatgtcccg      780

aatcgtctcg tttactcagc acacgactac gctacttccg tatacccgca aacttggttc      840

agcgacccga cattcccaaa taacatgccc ggtatctgga ataaaaattg gggttatctc      900

ttcaaccaaa acatcgcgcc cgtttggctt ggagaattcg gcactactct gcaatcgact      960

acagaccaaa cttggctcaa gactcttgtc cagtacctca gacctacagc acaatacgga     1020

gcagactcat ttcaatggac attttggtcc tggaacccgg attctggcga tactggcggt     1080

attcttaaag atgattggca aactgttgac actgtcaagg acggctacct cgcacctatc     1140

aaatcctcga tattcgatcc agttggc                                         1167


<210>  2
<211>  359
<212>  PRT
<213>  Acidothermus cellulolyticus


<220>
<221>  MISC_FEATURE
<222>  (1)..(359)
<223>  E1 endo 1,4-beta-glucanase polypeptide

<400>  2

Ala Gly Gly Gly Tyr Trp His Thr Ser Gly Arg Glu Ile Leu Asp Ala 
1               5                   10                  15      


Asn Asn Val Pro Val Arg Ile Ala Gly Ile Asn Trp Phe Gly Phe Glu 
            20                  25                  30          


Thr Cys Asn Tyr Val Val His Gly Leu Trp Ser Arg Asp Tyr Arg Ser 
        35                  40                  45              


Met Leu Asp Gln Ile Lys Ser Leu Gly Tyr Asn Thr Ile Arg Leu Pro 
    50                  55                  60                  


Tyr Ser Asp Asp Ile Leu Lys Pro Gly Thr Met Pro Asn Ser Ile Asn 
65                  70                  75                  80  


Phe Tyr Gln Met Asn Gln Asp Leu Gln Gly Leu Thr Ser Leu Gln Val 
                85                  90                  95      


Met Asp Lys Ile Val Ala Tyr Ala Gly Gln Ile Gly Leu Arg Ile Ile 
            100                 105                 110         


Leu Asp Arg His Arg Pro Asp Cys Ser Gly Gln Ser Ala Leu Trp Tyr 
        115                 120                 125             


Thr Ser Ser Val Ser Glu Ala Thr Trp Ile Ser Asp Leu Gln Ala Leu 
    130                 135                 140                 


Ala Gln Arg Tyr Lys Gly Asn Pro Thr Val Val Gly Phe Asp Leu His 
145                 150                 155                 160 


Asn Glu Pro His Asp Pro Ala Cys Trp Gly Cys Gly Asp Pro Ser Ile 
                165                 170                 175     


Asp Trp Arg Leu Ala Ala Glu Arg Ala Gly Asn Ala Val Leu Ser Val 
            180                 185                 190         


Asn Pro Asn Leu Leu Ile Phe Val Glu Gly Val Gln Ser Tyr Asn Gly 
        195                 200                 205             


Asp Ser Tyr Trp Trp Gly Gly Asn Leu Gln Gly Ala Gly Gln Tyr Pro 
    210                 215                 220                 


Val Val Leu Asn Val Pro Asn Arg Leu Val Tyr Ser Ala His Asp Tyr 
225                 230                 235                 240 


Ala Thr Ser Val Tyr Pro Gln Thr Trp Phe Ser Asp Pro Thr Phe Pro 
                245                 250                 255     


Asn Asn Met Pro Gly Ile Trp Asn Lys Asn Trp Gly Tyr Leu Phe Asn 
            260                 265                 270         


Gln Asn Ile Ala Pro Val Trp Leu Gly Glu Phe Gly Thr Thr Leu Gln 
        275                 280                 285             


Ser Thr Thr Asp Gln Thr Trp Leu Lys Thr Leu Val Gln Tyr Leu Arg 
    290                 295                 300                 


Pro Thr Ala Gln Tyr Gly Ala Asp Ser Phe Gln Trp Thr Phe Trp Ser 
305                 310                 315                 320 


Trp Asn Pro Asp Ser Gly Asp Thr Gly Gly Ile Leu Lys Asp Asp Trp 
                325                 330                 335     


Gln Thr Val Asp Thr Val Lys Asp Gly Tyr Leu Ala Pro Ile Lys Ser 
            340                 345                 350         


Ser Ile Phe Asp Pro Val Gly 
        355                 


<210>  3
<211>  1330
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon-optimized polynucleotide for CBH-E from Talaromyces 
       emersonii

<400>  3
ccatggatcc acagcaagcg ggtacggcca ccgcggagaa ccatcccccc cttacgtggc       60

aagaatgcac cgcccccgga tcgtgcacta ctcaaaatgg cgctgtggtt ctcgatgcta      120

actggcggtg ggttcacgat gttaatggtt acactaactg ctatacaggc aatacatggg      180

acccgaccta ctgccctgac gacgagactt gcgcccagaa ctgcgcactt gatggtgcgg      240

attatgaagg aacgtacgga gtcacctcct ccggctcttc ccttaagctt aatttcgtga      300

caggcagcaa tgtgggatca aggctctatc tgctccagga cgattctacc taccaaatat      360

tcaagctcct caacagagaa ttttccttcg acgtcgacgt ttctaatctc ccttgtggcc      420

tcaatggtgc actctatttc gtagccatgg acgcagacgg cggagtctcg aaatacccaa      480

acaacaaggc tggtgctaag tatggtacgg gatactgcga tagccagtgt ccacgcgatc      540

ttaaatttat tgacggtgaa gcaaacgtag aaggttggca gccatcatct aacaacgcaa      600

acacaggtat cggcgatcac ggcagctgtt gtgctgaaat ggacgtctgg gaagcaaact      660

caatatccaa tgcggttacc ccccatcctt gcgatacccc aggtcagacg atgtgctctg      720

gagacgattg tggtggaacc tactcgaatg accgctatgc cggcacctgc gatccagatg      780

gatgcgactt caatccctac cgcatgggta atacctcatt ctacggcccc ggaaaaataa      840

ttgacaccac gaagcctttc actgtagtaa ctcaattttt gactgacgac ggaacagaca      900

ccggtaccct gtccgagatc aaaagattct acatccagaa ttcaaacgtc atccctcaac      960

ctaatagcga catatcaggc gtgaccggta actcgataac aactgagttt tgcacagccc     1020

agaaacaagc gttcggcgac acagacgatt tctcccaaca cggaggcctg gcaaaaatgg     1080

gagctgcgat gcaacaaggc atggtactcg tgatgagtct ttgggatgat tatgctgcgc     1140

aaatgctttg gctggattcc gattatccga cagatgcaga cccaacaacc ccaggaatag     1200

ctagaggcac ctgcccaact gattcaggcg taccgagcga tgtcgaaagc cagtctccta     1260

attcttacgt tacatactcc aatattaagt tcggaccaat taactctaca ttcacggcct     1320

caggagatct                                                            1330


<210>  4
<211>  455
<212>  PRT
<213>  Talaromyces emersonii


<220>
<221>  MISC_FEATURE
<222>  (1)..(455)
<223>  CBH-E polypeptide

<400>  4

Met Leu Arg Arg Ala Leu Leu Leu Ser Ser Ser Ala Ile Leu Ala Val 
1               5                   10                  15      


Lys Ala Gln Gln Ala Gly Thr Ala Thr Ala Glu Asn His Pro Pro Leu 
            20                  25                  30          


Thr Trp Gln Glu Cys Thr Ala Pro Gly Ser Cys Thr Thr Gln Asn Gly 
        35                  40                  45              


Ala Val Val Leu Asp Ala Asn Trp Arg Trp Val His Asp Val Asn Gly 
    50                  55                  60                  


Tyr Thr Asn Cys Tyr Thr Gly Asn Thr Trp Asp Pro Thr Tyr Cys Pro 
65                  70                  75                  80  


Asp Asp Glu Thr Cys Ala Gln Asn Cys Ala Leu Asp Gly Ala Asp Tyr 
                85                  90                  95      


Glu Gly Thr Tyr Gly Val Thr Ser Ser Gly Ser Ser Leu Lys Leu Asn 
            100                 105                 110         


Phe Val Thr Gly Ser Asn Val Gly Ser Arg Leu Tyr Leu Leu Gln Asp 
        115                 120                 125             


Asp Ser Thr Tyr Gln Ile Phe Lys Leu Leu Asn Arg Glu Phe Ser Phe 
    130                 135                 140                 


Asp Val Asp Val Ser Asn Leu Pro Cys Gly Leu Asn Gly Ala Leu Tyr 
145                 150                 155                 160 


Phe Val Ala Met Asp Ala Asp Gly Gly Val Ser Lys Tyr Pro Asn Asn 
                165                 170                 175     


Lys Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser Gln Cys Pro 
            180                 185                 190         


Arg Asp Leu Lys Phe Ile Asp Gly Glu Ala Asn Val Glu Gly Trp Gln 
        195                 200                 205             


Pro Ser Ser Asn Asn Ala Asn Thr Gly Ile Gly Asp His Gly Ser Cys 
    210                 215                 220                 


Cys Ala Glu Met Asp Val Trp Glu Ala Asn Ser Ile Ser Asn Ala Val 
225                 230                 235                 240 


Thr Pro His Pro Cys Asp Thr Pro Gly Gln Thr Met Cys Ser Gly Asp 
                245                 250                 255     


Asp Cys Gly Gly Thr Tyr Ser Asn Asp Arg Tyr Ala Gly Thr Cys Asp 
            260                 265                 270         


Pro Asp Gly Cys Asp Phe Asn Pro Tyr Arg Met Gly Asn Thr Ser Phe 
        275                 280                 285             


Tyr Gly Pro Gly Lys Ile Ile Asp Thr Thr Lys Pro Phe Thr Val Val 
    290                 295                 300                 


Thr Gln Phe Leu Thr Asp Asp Gly Thr Asp Thr Gly Thr Leu Ser Glu 
305                 310                 315                 320 


Ile Lys Arg Phe Tyr Ile Gln Asn Ser Asn Val Ile Pro Gln Pro Asn 
                325                 330                 335     


Ser Asp Ile Ser Gly Val Thr Gly Asn Ser Ile Thr Thr Glu Phe Cys 
            340                 345                 350         


Thr Ala Gln Lys Gln Ala Phe Gly Asp Thr Asp Asp Phe Ser Gln His 
        355                 360                 365             


Gly Gly Leu Ala Lys Met Gly Ala Ala Met Gln Gln Gly Met Val Leu 
    370                 375                 380                 


Val Met Ser Leu Trp Asp Asp Tyr Ala Ala Gln Met Leu Trp Leu Asp 
385                 390                 395                 400 


Ser Asp Tyr Pro Thr Asp Ala Asp Pro Thr Thr Pro Gly Ile Ala Arg 
                405                 410                 415     


Gly Thr Cys Pro Thr Asp Ser Gly Val Pro Ser Asp Val Glu Ser Gln 
            420                 425                 430         


Ser Pro Asn Ser Tyr Val Thr Tyr Ser Asn Ile Lys Phe Gly Pro Ile 
        435                 440                 445             


Asn Ser Thr Phe Thr Ala Ser 
    450                 455 


<210>  5
<211>  1124
<212>  PRT
<213>  Acidothermus cellulolyticus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1124)
<223>  gux1 polypeptide

<400>  5

Met Gly Ala Pro Gly Leu Arg Arg Arg Leu Arg Ala Gly Ile Val Ser 
1               5                   10                  15      


Ala Ala Ala Leu Gly Ser Leu Val Ser Gly Leu Val Ala Val Ala Pro 
            20                  25                  30          


Val Ala His Ala Ala Val Thr Leu Lys Ala Gln Tyr Lys Asn Asn Asp 
        35                  40                  45              


Ser Ala Pro Ser Asp Asn Gln Ile Lys Pro Gly Leu Gln Leu Val Asn 
    50                  55                  60                  


Thr Gly Ser Ser Ser Val Asp Leu Ser Thr Val Thr Val Arg Tyr Trp 
65                  70                  75                  80  


Phe Thr Arg Asp Gly Gly Ser Ser Thr Leu Val Tyr Asn Cys Asp Trp 
                85                  90                  95      


Ala Ala Met Gly Cys Gly Asn Ile Arg Ala Ser Phe Gly Ser Val Asn 
            100                 105                 110         


Pro Ala Thr Pro Thr Ala Asp Thr Tyr Leu Gln Leu Ser Phe Thr Gly 
        115                 120                 125             


Gly Thr Leu Ala Ala Gly Gly Ser Thr Gly Glu Ile Gln Asn Arg Val 
    130                 135                 140                 


Asn Lys Ser Asp Trp Ser Asn Phe Asp Glu Thr Asn Asp Tyr Ser Tyr 
145                 150                 155                 160 


Gly Thr Asn Thr Thr Phe Gln Asp Trp Thr Lys Val Thr Val Tyr Val 
                165                 170                 175     


Asn Gly Val Leu Val Trp Gly Thr Glu Pro Ser Gly Ala Thr Ala Ser 
            180                 185                 190         


Pro Ser Ala Ser Ala Thr Pro Ser Pro Ser Ser Ser Pro Thr Thr Ser 
        195                 200                 205             


Pro Ser Ser Ser Pro Ser Pro Ser Ser Ser Pro Thr Pro Thr Pro Ser 
    210                 215                 220                 


Ser Ser Ser Pro Pro Pro Ser Ser Asn Asp Pro Tyr Ile Gln Arg Phe 
225                 230                 235                 240 


Leu Thr Met Tyr Asn Lys Ile His Asp Pro Ala Asn Gly Tyr Phe Ser 
                245                 250                 255     


Pro Gln Gly Ile Pro Tyr His Ser Val Glu Thr Leu Ile Val Glu Ala 
            260                 265                 270         


Pro Asp Tyr Gly His Glu Thr Thr Ser Glu Ala Tyr Ser Phe Trp Leu 
        275                 280                 285             


Trp Leu Glu Ala Thr Tyr Gly Ala Val Thr Gly Asn Trp Thr Pro Phe 
    290                 295                 300                 


Asn Asn Ala Trp Thr Thr Met Glu Thr Tyr Met Ile Pro Gln His Ala 
305                 310                 315                 320 


Asp Gln Pro Asn Asn Ala Ser Tyr Asn Pro Asn Ser Pro Ala Ser Tyr 
                325                 330                 335     


Ala Pro Glu Glu Pro Leu Pro Ser Met Tyr Pro Val Ala Ile Asp Ser 
            340                 345                 350         


Ser Val Pro Val Gly His Asp Pro Leu Ala Ala Glu Leu Gln Ser Thr 
        355                 360                 365             


Tyr Gly Thr Pro Asp Ile Tyr Gly Met His Trp Leu Ala Asp Val Asp 
    370                 375                 380                 


Asn Ile Tyr Gly Tyr Gly Asp Ser Pro Gly Gly Gly Cys Glu Leu Gly 
385                 390                 395                 400 


Pro Ser Ala Lys Gly Val Ser Tyr Ile Asn Thr Phe Gln Arg Gly Ser 
                405                 410                 415     


Gln Glu Ser Val Trp Glu Thr Val Thr Gln Pro Thr Cys Asp Asn Gly 
            420                 425                 430         


Lys Tyr Gly Gly Ala His Gly Tyr Val Asp Leu Phe Ile Gln Gly Ser 
        435                 440                 445             


Thr Pro Pro Gln Trp Lys Tyr Thr Asp Ala Pro Asp Ala Asp Ala Arg 
    450                 455                 460                 


Ala Val Gln Ala Ala Tyr Trp Ala Tyr Thr Trp Ala Ser Ala Gln Gly 
465                 470                 475                 480 


Lys Ala Ser Ala Ile Ala Pro Thr Ile Ala Lys Ala Ala Lys Leu Gly 
                485                 490                 495     


Asp Tyr Leu Arg Tyr Ser Leu Phe Asp Lys Tyr Phe Lys Gln Val Gly 
            500                 505                 510         


Asn Cys Tyr Pro Ala Ser Ser Cys Pro Gly Ala Thr Gly Arg Gln Ser 
        515                 520                 525             


Glu Thr Tyr Leu Ile Gly Trp Tyr Tyr Ala Trp Gly Gly Ser Ser Gln 
    530                 535                 540                 


Gly Trp Ala Trp Arg Ile Gly Asp Gly Ala Ala His Phe Gly Tyr Gln 
545                 550                 555                 560 


Asn Pro Leu Ala Ala Trp Ala Met Ser Asn Val Thr Pro Leu Ile Pro 
                565                 570                 575     


Leu Ser Pro Thr Ala Lys Ser Asp Trp Ala Ala Ser Leu Gln Arg Gln 
            580                 585                 590         


Leu Glu Phe Tyr Gln Trp Leu Gln Ser Ala Glu Gly Ala Ile Ala Gly 
        595                 600                 605             


Gly Ala Thr Asn Ser Trp Asn Gly Asn Tyr Gly Thr Pro Pro Ala Gly 
    610                 615                 620                 


Asp Ser Thr Phe Tyr Gly Met Ala Tyr Asp Trp Glu Pro Val Tyr His 
625                 630                 635                 640 


Asp Pro Pro Ser Asn Asn Trp Phe Gly Phe Gln Ala Trp Ser Met Glu 
                645                 650                 655     


Arg Val Ala Glu Tyr Tyr Tyr Val Thr Gly Asp Pro Lys Ala Lys Ala 
            660                 665                 670         


Leu Leu Asp Lys Trp Val Ala Trp Val Lys Pro Asn Val Thr Thr Gly 
        675                 680                 685             


Ala Ser Trp Ser Ile Pro Ser Asn Leu Ser Trp Ser Gly Gln Pro Asp 
    690                 695                 700                 


Thr Trp Asn Pro Ser Asn Pro Gly Thr Asn Ala Asn Leu His Val Thr 
705                 710                 715                 720 


Ile Thr Ser Ser Gly Gln Asp Val Gly Val Ala Ala Ala Leu Ala Lys 
                725                 730                 735     


Thr Leu Glu Tyr Tyr Ala Ala Lys Ser Gly Asp Thr Ala Ser Arg Asp 
            740                 745                 750         


Leu Ala Lys Gly Leu Leu Asp Ser Ile Trp Asn Asn Asp Gln Asp Ser 
        755                 760                 765             


Leu Gly Val Ser Thr Pro Glu Thr Arg Thr Asp Tyr Ser Arg Phe Thr 
    770                 775                 780                 


Gln Val Tyr Asp Pro Thr Thr Gly Asp Gly Leu Tyr Ile Pro Ser Gly 
785                 790                 795                 800 


Trp Thr Gly Thr Met Pro Asn Gly Asp Gln Ile Lys Pro Gly Ala Thr 
                805                 810                 815     


Phe Leu Ser Ile Arg Ser Trp Tyr Thr Lys Asp Pro Gln Trp Ser Lys 
            820                 825                 830         


Val Gln Ala Tyr Leu Asn Gly Gly Pro Ala Pro Thr Phe Asn Tyr His 
        835                 840                 845             


Arg Phe Trp Ala Glu Ser Asp Phe Ala Met Ala Asn Ala Asp Phe Gly 
    850                 855                 860                 


Met Leu Phe Pro Ser Gly Ser Pro Ser Pro Thr Pro Ser Pro Thr Pro 
865                 870                 875                 880 


Thr Ser Ser Pro Ser Pro Thr Pro Ser Ser Ser Pro Thr Pro Ser Pro 
                885                 890                 895     


Ser Pro Ser Pro Thr Gly Asp Thr Thr Pro Pro Ser Val Pro Thr Gly 
            900                 905                 910         


Leu Gln Val Thr Gly Thr Thr Thr Ser Ser Val Ser Leu Ser Trp Thr 
        915                 920                 925             


Ala Ser Thr Asp Asn Val Gly Val Ala His Tyr Asn Val Tyr Arg Asn 
    930                 935                 940                 


Gly Thr Leu Val Gly Gln Pro Thr Ala Thr Ser Phe Thr Asp Thr Gly 
945                 950                 955                 960 


Leu Ala Ala Gly Thr Ser Tyr Thr Tyr Thr Val Ala Ala Val Asp Ala 
                965                 970                 975     


Ala Gly Asn Thr Ser Ala Gln Ser Ser Pro Val Thr Ala Thr Thr Ala 
            980                 985                 990         


Ser Pro Ser Pro Ser Pro Ser Pro  Ser Pro Thr Pro Thr  Ser Ser Pro 
        995                 1000                 1005             


Ser Pro  Thr Pro Ser Pro Thr  Pro Ser Pro Thr Ser  Thr Ser Gly 
    1010                 1015                 1020             


Ala Ser  Cys Thr Ala Thr Tyr  Val Val Asn Ser Asp  Trp Gly Ser 
    1025                 1030                 1035             


Gly Phe  Thr Thr Thr Val Thr  Val Thr Asn Thr Gly  Thr Arg Ala 
    1040                 1045                 1050             


Thr Ser  Gly Trp Thr Val Thr  Trp Ser Phe Ala Gly  Asn Gln Thr 
    1055                 1060                 1065             


Val Thr  Asn Tyr Trp Asn Thr  Ala Leu Thr Gln Ser  Gly Lys Ser 
    1070                 1075                 1080             


Val Thr  Ala Lys Asn Leu Ser  Tyr Asn Asn Val Ile  Gln Pro Gly 
    1085                 1090                 1095             


Gln Ser  Thr Thr Phe Gly Phe  Asn Gly Ser Tyr Ser  Gly Thr Asn 
    1100                 1105                 1110             


Thr Ala  Pro Thr Leu Ser Cys  Thr Ala Ser Glx 
    1115                 1120                 


<210>  6
<211>  683
<212>  PRT
<213>  Acidothermus cellulolyticus


<220>
<221>  MISC_FEATURE
<222>  (1)..(683)
<223>  XylE polypeptide

<400>  6

Met Gly His His Ala Met Arg Arg Met Val Thr Ser Ala Ser Val Val 
1               5                   10                  15      


Gly Val Ala Thr Leu Ala Ala Ala Thr Val Leu Ile Thr Gly Gly Ile 
            20                  25                  30          


Ala His Ala Ala Ser Thr Leu Lys Gln Gly Ala Glu Ala Asn Gly Arg 
        35                  40                  45              


Tyr Phe Gly Val Ser Ala Ser Val Asn Thr Leu Asn Asn Ser Ala Ala 
    50                  55                  60                  


Ala Asn Leu Val Ala Thr Gln Phe Asp Met Leu Thr Pro Glu Asn Glu 
65                  70                  75                  80  


Met Lys Trp Asp Thr Val Glu Ser Ser Arg Gly Ser Phe Asn Phe Gly 
                85                  90                  95      


Pro Gly Asp Gln Ile Val Ala Phe Ala Thr Ala His Asn Met Arg Val 
            100                 105                 110         


Arg Gly His Asn Leu Val Trp His Ser Gln Leu Pro Gly Trp Val Ser 
        115                 120                 125             


Ser Leu Pro Leu Ser Gln Val Gln Ser Ala Met Glu Ser His Ile Thr 
    130                 135                 140                 


Ala Glu Val Thr His Tyr Lys Gly Lys Ile Tyr Ala Trp Asp Val Val 
145                 150                 155                 160 


Asn Glu Pro Phe Asp Asp Ser Gly Asn Leu Arg Thr Asp Val Phe Tyr 
                165                 170                 175     


Gln Ala Met Gly Ala Gly Tyr Ile Ala Asp Ala Leu Arg Thr Ala His 
            180                 185                 190         


Ala Ala Asp Pro Asn Ala Lys Leu Tyr Leu Asn Asp Tyr Asn Ile Glu 
        195                 200                 205             


Gly Ile Asn Ala Lys Ser Asp Ala Met Tyr Asn Leu Ile Lys Gln Leu 
    210                 215                 220                 


Lys Ser Gln Gly Val Pro Ile Asp Gly Val Gly Phe Glu Ser His Phe 
225                 230                 235                 240 


Ile Val Gly Gln Val Pro Ser Thr Leu Gln Gln Asn Met Gln Arg Phe 
                245                 250                 255     


Ala Asp Leu Gly Val Asp Val Ala Ile Thr Glu Leu Asp Asp Arg Met 
            260                 265                 270         


Pro Thr Pro Pro Ser Gln Gln Asn Leu Asn Gln Gln Ala Thr Asp Asp 
        275                 280                 285             


Ala Asn Val Val Lys Ala Cys Leu Ala Val Ala Arg Cys Val Gly Ile 
    290                 295                 300                 


Thr Gln Trp Asp Val Ser Asp Ala Asp Ser Trp Val Pro Gly Thr Phe 
305                 310                 315                 320 


Ser Gly Gln Gly Ala Ala Thr Met Phe Asp Ser Asn Leu Gln Pro Lys 
                325                 330                 335     


Pro Ala Phe Thr Ala Val Leu Asn Ala Leu Ser Ala Ser Ala Ser Val 
            340                 345                 350         


Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro 
        355                 360                 365             


Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro 
    370                 375                 380                 


Ser Ser Ser Pro Val Ser Gly Gly Val Lys Val Gln Tyr Lys Asn Asn 
385                 390                 395                 400 


Asp Ser Ala Pro Gly Asp Asn Gln Ile Lys Pro Gly Leu Gln Val Val 
                405                 410                 415     


Asn Thr Gly Ser Ser Ser Val Asp Leu Ser Thr Val Thr Val Arg Tyr 
            420                 425                 430         


Trp Phe Thr Arg Asp Gly Gly Ser Ser Thr Leu Val Tyr Asn Cys Asp 
        435                 440                 445             


Trp Ala Val Met Gly Cys Gly Asn Ile Arg Ala Ser Phe Gly Ser Val 
    450                 455                 460                 


Asn Pro Ala Thr Pro Thr Ala Asp Thr Tyr Leu Gln Leu Ser Phe Thr 
465                 470                 475                 480 


Gly Gly Thr Leu Pro Ala Gly Gly Ser Thr Gly Glu Ile Gln Ser Arg 
                485                 490                 495     


Val Asn Lys Ser Asp Trp Ser Asn Phe Thr Glu Thr Asn Asp Tyr Ser 
            500                 505                 510         


Tyr Gly Thr Asn Thr Thr Phe Gln Asp Trp Ser Lys Val Thr Val Tyr 
        515                 520                 525             


Val Asn Gly Arg Leu Val Trp Gly Thr Glu Pro Ser Gly Thr Ser Pro 
    530                 535                 540                 


Ser Pro Thr Pro Ser Pro Ser Pro Thr Pro Ser Pro Ser Pro Ser Pro 
545                 550                 555                 560 


Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro Ser Pro 
                565                 570                 575     


Ser Ser Ser Pro Ser Ser Gly Cys Val Ala Ser Met Arg Val Asp Ser 
            580                 585                 590         


Ser Trp Pro Gly Gly Phe Thr Ala Thr Val Thr Val Ser Asn Thr Gly 
        595                 600                 605             


Gly Val Ser Thr Ser Gly Trp Gln Val Gly Trp Ser Trp Pro Ser Gly 
    610                 615                 620                 


Asp Ser Leu Val Asn Ala Trp Asn Ala Val Val Ser Val Thr Gly Thr 
625                 630                 635                 640 


Ser Val Arg Ala Val Asn Ala Ser Tyr Asn Gly Val Ile Pro Ala Gly 
                645                 650                 655     


Gly Ser Thr Thr Phe Gly Phe Gln Ala Asn Gly Thr Pro Gly Thr Pro 
            660                 665                 670         


Thr Phe Thr Cys Thr Thr Ser Ala Asp Leu Glx 
        675                 680             


<210>  7
<211>  1256
<212>  PRT
<213>  Acidothermus cellulolyticus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1256)
<223>  aviIII polypeptide

<400>  7

Met Ala Ala Thr Thr Gln Pro Tyr Thr Trp Ser Asn Val Ala Ile Gly 
1               5                   10                  15      


Gly Gly Gly Phe Val Asp Gly Ile Val Phe Asn Glu Gly Ala Pro Gly 
            20                  25                  30          


Ile Leu Tyr Val Arg Thr Asp Ile Gly Gly Met Tyr Arg Trp Asp Ala 
        35                  40                  45              


Ala Asn Gly Arg Trp Ile Pro Leu Leu Asp Trp Val Gly Trp Asn Asn 
    50                  55                  60                  


Trp Gly Tyr Asn Gly Val Val Ser Ile Ala Ala Asp Pro Ile Asn Thr 
65                  70                  75                  80  


Asn Lys Val Trp Ala Ala Val Gly Met Tyr Thr Asn Ser Trp Asp Pro 
                85                  90                  95      


Asn Asp Gly Ala Ile Leu Arg Ser Ser Asp Gln Gly Ala Thr Trp Gln 
            100                 105                 110         


Ile Thr Pro Leu Pro Phe Lys Leu Gly Gly Asn Met Pro Gly Arg Gly 
        115                 120                 125             


Met Gly Glu Arg Leu Ala Val Asp Pro Asn Asn Asp Asn Ile Leu Tyr 
    130                 135                 140                 


Phe Gly Ala Pro Ser Gly Lys Gly Leu Trp Arg Ser Thr Asp Ser Gly 
145                 150                 155                 160 


Ala Thr Trp Ser Gln Met Thr Asn Phe Pro Asp Val Gly Thr Tyr Ile 
                165                 170                 175     


Ala Asn Pro Thr Asp Thr Thr Gly Tyr Gln Ser Asp Ile Gln Gly Val 
            180                 185                 190         


Val Trp Val Ala Phe Asp Lys Ser Ser Ser Ser Leu Gly Gln Ala Ser 
        195                 200                 205             


Lys Thr Ile Phe Val Gly Val Ala Asp Pro Asn Asn Pro Val Phe Trp 
    210                 215                 220                 


Ser Arg Asp Gly Gly Ala Thr Trp Gln Ala Val Pro Gly Ala Pro Thr 
225                 230                 235                 240 


Gly Phe Ile Pro His Lys Gly Val Phe Asp Pro Val Asn His Val Leu 
                245                 250                 255     


Tyr Ile Ala Thr Ser Asn Thr Gly Gly Pro Tyr Asp Gly Ser Ser Gly 
            260                 265                 270         


Asp Val Trp Lys Phe Ser Val Thr Ser Gly Thr Trp Thr Arg Ile Ser 
        275                 280                 285             


Pro Val Pro Ser Thr Asp Thr Ala Asn Asp Tyr Phe Gly Tyr Ser Gly 
    290                 295                 300                 


Leu Thr Ile Asp Arg Gln His Pro Asn Thr Ile Met Val Ala Thr Gln 
305                 310                 315                 320 


Ile Ser Trp Trp Pro Asp Thr Ile Ile Phe Arg Ser Thr Asp Gly Gly 
                325                 330                 335     


Ala Thr Trp Thr Arg Ile Trp Asp Trp Thr Ser Tyr Pro Asn Arg Ser 
            340                 345                 350         


Leu Arg Tyr Val Leu Asp Ile Ser Ala Glu Pro Trp Leu Thr Phe Gly 
        355                 360                 365             


Val Gln Pro Asn Pro Pro Val Pro Ser Pro Lys Leu Gly Trp Met Asp 
    370                 375                 380                 


Glu Ala Met Ala Ile Asp Pro Phe Asn Ser Asp Arg Met Leu Tyr Gly 
385                 390                 395                 400 


Thr Gly Ala Thr Leu Tyr Ala Thr Asn Asp Leu Thr Lys Trp Asp Ser 
                405                 410                 415     


Gly Gly Gln Ile His Ile Ala Pro Met Val Lys Gly Leu Glu Glu Thr 
            420                 425                 430         


Ala Val Asn Asp Leu Ile Ser Pro Pro Ser Gly Ala Pro Leu Ile Ser 
        435                 440                 445             


Ala Leu Gly Asp Leu Gly Gly Phe Thr His Ala Asp Val Thr Ala Val 
    450                 455                 460                 


Pro Ser Thr Ile Phe Thr Ser Pro Val Phe Thr Thr Gly Thr Ser Val 
465                 470                 475                 480 


Asp Tyr Ala Glu Leu Asn Pro Ser Ile Ile Val Arg Ala Gly Ser Phe 
                485                 490                 495     


Asp Pro Ser Ser Gln Pro Asn Asp Arg His Val Ala Phe Ser Thr Asp 
            500                 505                 510         


Gly Gly Lys Asn Trp Phe Gln Gly Ser Glu Pro Gly Gly Val Thr Thr 
        515                 520                 525             


Gly Gly Thr Val Ala Ala Ser Ala Asp Gly Ser Arg Phe Val Trp Ala 
    530                 535                 540                 


Pro Gly Asp Pro Gly Gln Pro Val Val Tyr Ala Val Gly Phe Gly Asn 
545                 550                 555                 560 


Ser Trp Ala Ala Ser Gln Gly Val Pro Ala Asn Ala Gln Ile Arg Ser 
                565                 570                 575     


Asp Arg Val Asn Pro Lys Thr Phe Tyr Ala Leu Ser Asn Gly Thr Phe 
            580                 585                 590         


Tyr Arg Ser Thr Asp Gly Gly Val Thr Phe Gln Pro Val Ala Ala Gly 
        595                 600                 605             


Leu Pro Ser Ser Gly Ala Val Gly Val Met Phe His Ala Val Pro Gly 
    610                 615                 620                 


Lys Glu Gly Asp Leu Trp Leu Ala Ala Ser Ser Gly Leu Tyr His Ser 
625                 630                 635                 640 


Thr Asn Gly Gly Ser Ser Trp Ser Ala Ile Thr Gly Val Ser Ser Ala 
                645                 650                 655     


Val Asn Val Gly Phe Gly Lys Ser Ala Pro Gly Ser Ser Tyr Pro Ala 
            660                 665                 670         


Val Phe Val Val Gly Thr Ile Gly Gly Val Thr Gly Ala Tyr Arg Ser 
        675                 680                 685             


Asp Asp Gly Gly Thr Thr Trp Val Arg Ile Asn Asp Asp Gln His Gln 
    690                 695                 700                 


Tyr Gly Asn Trp Gly Gln Ala Ile Thr Gly Asp Pro Arg Ile Tyr Gly 
705                 710                 715                 720 


Arg Val Tyr Ile Gly Thr Asn Gly Arg Gly Ile Val Tyr Gly Asp Ile 
                725                 730                 735     


Ala Gly Ala Pro Ser Gly Ser Pro Ser Pro Ser Val Ser Pro Ser Ala 
            740                 745                 750         


Ser Pro Ser Leu Ser Pro Ser Pro Ser Pro Ser Ser Ser Pro Ser Pro 
        755                 760                 765             


Ser Pro Ser Pro Ser Ser Ser Pro Ser Ser Ser Pro Ser Pro Ser Pro 
    770                 775                 780                 


Ser Pro Ser Pro Ser Pro Ser Arg Ser Pro Ser Pro Ser Ala Ser Pro 
785                 790                 795                 800 


Ser Pro Ser Ser Ser Pro Ser Pro Ser Ser Ser Pro Ser Ser Ser Pro 
                805                 810                 815     


Ser Pro Thr Pro Ser Ser Ser Pro Val Ser Gly Gly Val Lys Val Gln 
            820                 825                 830         


Tyr Lys Asn Asn Asp Ser Ala Pro Gly Asp Asn Gln Ile Lys Pro Gly 
        835                 840                 845             


Leu Gln Val Val Asn Thr Gly Ser Ser Ser Val Asp Leu Ser Thr Val 
    850                 855                 860                 


Thr Val Arg Tyr Trp Phe Thr Arg Asp Gly Gly Ser Ser Thr Leu Val 
865                 870                 875                 880 


Tyr Asn Cys Asp Trp Ala Ala Ile Gly Cys Gly Asn Ile Arg Ala Ser 
                885                 890                 895     


Phe Gly Ser Val Asn Pro Ala Thr Pro Thr Ala Asp Thr Tyr Leu Gln 
            900                 905                 910         


Leu Ser Phe Thr Gly Gly Thr Leu Ala Ala Gly Gly Ser Thr Gly Glu 
        915                 920                 925             


Ile Gln Asn Arg Val Asn Lys Ser Asp Trp Ser Asn Phe Thr Glu Thr 
    930                 935                 940                 


Asn Asp Tyr Ser Tyr Gly Thr Asn Thr Val Phe Gln Asp Trp Ser Lys 
945                 950                 955                 960 


Val Thr Val Tyr Val Asn Gly Arg Leu Val Trp Gly Thr Glu Pro Ser 
                965                 970                 975     


Gly Thr Ser Pro Ser Pro Thr Pro Ser Pro Ser Pro Thr Pro Ser Pro 
            980                 985                 990         


Ser Pro Ser Pro Ser Pro Gly Gly  Asp Val Thr Pro Pro  Ser Val Pro 
        995                 1000                 1005             


Thr Gly  Val Val Val Thr Gly  Val Ser Gly Ser Ser  Val Ser Leu 
    1010                 1015                 1020             


Ala Trp  Asn Ala Ser Thr Asp  Asn Val Gly Val Ala  His Tyr Asn 
    1025                 1030                 1035             


Val Tyr  Arg Asn Gly Val Leu  Val Gly Gln Pro Thr  Val Thr Ser 
    1040                 1045                 1050             


Phe Thr  Asp Thr Gly Leu Ala  Ala Gly Thr Ala Tyr  Thr Tyr Thr 
    1055                 1060                 1065             


Val Ala  Ala Val Asp Ala Ala  Gly Asn Thr Ser Ala  Pro Ser Thr 
    1070                 1075                 1080             


Pro Val  Thr Ala Thr Thr Thr  Ser Pro Ser Pro Ser  Pro Ser Pro 
    1085                 1090                 1095             


Thr Pro  Ser Pro Thr Pro Ser  Pro Thr Pro Ser Pro  Ser Pro Ser 
    1100                 1105                 1110             


Pro Ser  Leu Ser Pro Ser Pro  Ser Pro Ser Pro Ser  Pro Ser Pro 
    1115                 1120                 1125             


Ser Pro  Ser Leu Ser Pro Ser  Pro Ser Thr Ser Pro  Ser Pro Ser 
    1130                 1135                 1140             


Pro Ser  Pro Thr Pro Ser Pro  Ser Ser Ser Gly Val  Gly Cys Arg 
    1145                 1150                 1155             


Ala Thr  Tyr Val Val Asn Ser  Asp Trp Gly Ser Gly  Phe Thr Ala 
    1160                 1165                 1170             


Thr Val  Thr Val Thr Asn Thr  Gly Ser Arg Ala Thr  Ser Gly Trp 
    1175                 1180                 1185             


Thr Val  Ala Trp Ser Phe Gly  Gly Asn Gln Thr Val  Thr Asn Tyr 
    1190                 1195                 1200             


Trp Asn  Thr Leu Leu Thr Gln  Ser Gly Ala Ser Val  Thr Ala Thr 
    1205                 1210                 1215             


Asn Leu  Ser Tyr Asn Asn Val  Ile Gln Pro Gly Gln  Ser Thr Thr 
    1220                 1225                 1230             


Phe Gly  Phe Asn Ala Thr Tyr  Ala Gly Thr Asn Thr  Pro Pro Thr 
    1235                 1240                 1245             


Pro Thr  Cys Thr Thr Asn Ser  Asp 
    1250                 1255     


<210>  8
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic - forward primer for CBH-E glucanase from Talaromyces 
       emersonii

<400>  8
acaggcagca atgtgggatc aa                                                22


<210>  9
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic - reverse primer for CBH-E glucanase from Talaromyces 
       emersonii

<400>  9
tgttgcatcg cagctcccat tt                                                22


<210>  10
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic - forward primer for marker gene (D35S 
       promoter-neomycin phosphotransferase II derived from bacterial 
       transposon Tn5)

<400>  10
ttcatttcat ttggagagga ca                                                22


<210>  11
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic - reverse primer for marker gene (D35S 
       promoter-neomycin phosphotransferase II derived from bacterial 
       transposon Tn5)

<400>  11
caagctcttc agcaatatca cg                                                22


