                         SEQUENCE LISTING

<110>  Medizinische Universitt Innsbruck
 
<120>  Antagonists of NR2F6 for augmenting the immune response

<130>  P2516 PCT S3

<150>  EP 08 01 2631.1
<151>  2008-07-11

<160>  33  

<170>  PatentIn version 3.5

<210>  1
<211>  1804
<212>  DNA
<213>  Homo sapiens

<400>  1
gtgcagcccg tgccccccgc gcgccggggc cgaatgcgcg ccgcgtaggg tcccccgggc     60

cgagaggggt gcccggaggg aagagcgcgg tgggggcgcc ccggccccgc tgccctgggg    120

ctatggccat ggtgaccggc ggctggggcg gccccggcgg cgacacgaac ggcgtggaca    180

aggcgggcgg ctacccgcgc gcggccgagg acgactcggc ctcgcccccc ggtgccgcca    240

gcgacgccga gccgggcgac gaggagcggc cggggctgca ggtggactgc gtggtgtgcg    300

gggacaagtc gagcggcaag cattacggtg tcttcacctg cgagggctgc aagagctttt    360

tcaagcgaag catccgccgc aacctcagct acacctgccg gtccaaccgt gactgccaga    420

tcgaccagca ccaccggaac cagtgccagt actgccgtct caagaagtgc ttccgggtgg    480

gcatgaggaa ggaggcggtg cagcgcggcc gcatcccgca ctcgctgcct ggtgccgtgg    540

ccgcctcctc gggcagcccc ccgggctcgg cgctggcggc agtggcgagc ggcggagacc    600

tcttcccggg gcagccggtg tccgaactga tcgcgcagct gctgcgcgct gagccctacc    660

ctgcggcggc cggacgcttc ggcgcagggg gcggcgcggc gggcgcggtg ctgggcatcg    720

acaacgtgtg cgagctggcg gcgcggctgc tcttcagcac cgtggagtgg gcgcgccacg    780

cgcccttctt ccccgagctg ccggtggccg accaggtggc gctgctgcgc ctgagctgga    840

gcgagctctt cgtgctgaac gcggcgcagg cggcgctgcc cctgcacacg gcgccgctac    900

tggccgccgc cggcctccac gccgcgccta tggccgccga gcgcgccgtg gctttcatgg    960

accaggtgcg cgccttccag gagcaggtgg acaagctggg ccgcctgcag gtcgactcgg   1020

ccgagtatgg ctgcctcaag gccatcgcgc tcttcacgcc cgacgcctgt ggcctctcag   1080

acccggccca cgttgagagc ctgcaggaga aggcgcaggt ggccctcacc gagtatgtgc   1140

gggcgcagta cccgtcccag ccccagcgct tcgggcgcct gctgctgcgg ctccccgccc   1200

tgcgcgcggt ccctgcctcc ctcatctccc agctgttctt catgcgcctg gtggggaaga   1260

cgcccattga gacactgatc agagacatgc tgctgtcggg gagtaccttc aactggccct   1320

acggctcggg ccagtgacca tgacggggcc acgtgtgctg tggccaggcc tgcagacaga   1380

cctcaaggga cagggaatgc tgaggcctcg aggggcctcc cggggcccag gactctggct   1440

tctctcctca gacttctatt ttttaaagac tgtgaaatgt ttgtcttttc tgttttttaa   1500

atgatcatga aaccaaaaag agactgatca tccaggcctc agcctcatcc tccccaggac   1560

ccctgtccag gatggagggt ccaatcctag gacagccttg ttcctcagca cccctagcat   1620

gaacttgtgg gatggtgggg ttggcttccc tggcatgatg gacaaaggcc tggcgtcggc   1680

cagaggggct gctccagtgg gcaggggtag ctagcgtgtg ccaggcagat cctctggaca   1740

cgtaacctat gtcagacact acatgatgac tcaaggccaa taataaagac atttcctacc   1800

tgca                                                                1804


<210>  2
<211>  404
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Met Val Thr Gly Gly Trp Gly Gly Pro Gly Gly Asp Thr Asn 
1               5                   10                  15      


Gly Val Asp Lys Ala Gly Gly Tyr Pro Arg Ala Ala Glu Asp Asp Ser 
            20                  25                  30          


Ala Ser Pro Pro Gly Ala Ala Ser Asp Ala Glu Pro Gly Asp Glu Glu 
        35                  40                  45              


Arg Pro Gly Leu Gln Val Asp Cys Val Val Cys Gly Asp Lys Ser Ser 
    50                  55                  60                  


Gly Lys His Tyr Gly Val Phe Thr Cys Glu Gly Cys Lys Ser Phe Phe 
65                  70                  75                  80  


Lys Arg Ser Ile Arg Arg Asn Leu Ser Tyr Thr Cys Arg Ser Asn Arg 
                85                  90                  95      


Asp Cys Gln Ile Asp Gln His His Arg Asn Gln Cys Gln Tyr Cys Arg 
            100                 105                 110         


Leu Lys Lys Cys Phe Arg Val Gly Met Arg Lys Glu Ala Val Gln Arg 
        115                 120                 125             


Gly Arg Ile Pro His Ser Leu Pro Gly Ala Val Ala Ala Ser Ser Gly 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ala Leu Ala Ala Val Ala Ser Gly Gly Asp Leu 
145                 150                 155                 160 


Phe Pro Gly Gln Pro Val Ser Glu Leu Ile Ala Gln Leu Leu Arg Ala 
                165                 170                 175     


Glu Pro Tyr Pro Ala Ala Ala Gly Arg Phe Gly Ala Gly Gly Gly Ala 
            180                 185                 190         


Ala Gly Ala Val Leu Gly Ile Asp Asn Val Cys Glu Leu Ala Ala Arg 
        195                 200                 205             


Leu Leu Phe Ser Thr Val Glu Trp Ala Arg His Ala Pro Phe Phe Pro 
    210                 215                 220                 


Glu Leu Pro Val Ala Asp Gln Val Ala Leu Leu Arg Leu Ser Trp Ser 
225                 230                 235                 240 


Glu Leu Phe Val Leu Asn Ala Ala Gln Ala Ala Leu Pro Leu His Thr 
                245                 250                 255     


Ala Pro Leu Leu Ala Ala Ala Gly Leu His Ala Ala Pro Met Ala Ala 
            260                 265                 270         


Glu Arg Ala Val Ala Phe Met Asp Gln Val Arg Ala Phe Gln Glu Gln 
        275                 280                 285             


Val Asp Lys Leu Gly Arg Leu Gln Val Asp Ser Ala Glu Tyr Gly Cys 
    290                 295                 300                 


Leu Lys Ala Ile Ala Leu Phe Thr Pro Asp Ala Cys Gly Leu Ser Asp 
305                 310                 315                 320 


Pro Ala His Val Glu Ser Leu Gln Glu Lys Ala Gln Val Ala Leu Thr 
                325                 330                 335     


Glu Tyr Val Arg Ala Gln Tyr Pro Ser Gln Pro Gln Arg Phe Gly Arg 
            340                 345                 350         


Leu Leu Leu Arg Leu Pro Ala Leu Arg Ala Val Pro Ala Ser Leu Ile 
        355                 360                 365             


Ser Gln Leu Phe Phe Met Arg Leu Val Gly Lys Thr Pro Ile Glu Thr 
    370                 375                 380                 


Leu Ile Arg Asp Met Leu Leu Ser Gly Ser Thr Phe Asn Trp Pro Tyr 
385                 390                 395                 400 


Gly Ser Gly Gln 
                


<210>  3
<211>  1061
<212>  DNA
<213>  Homo sapiens

<400>  3
cggctgctct tcagcaccgt ggagtgggcg cgccacgcgc ccttcttccc cgagctgccg     60

gtggccgacc aggtggcgct gctgcgcctg agctggagcg agctcttcgt gctgaacgcg    120

gcgcaggcgg cgctgcccct gcacacggcg ccgctactgg ccgccgccgg cctccacgcc    180

gcgcctatgg ccgccgagcg cgccgtggct ttcatggacc aggtgcgcgc cttccaggag    240

caggtggaca agctgggccg cctgcaggtc gactcggccg agtatggctg cctcaaggcc    300

atcgcgctct tcacgcccga cgcctgtggc ctctcagacc cggcccacgt tgagagcctg    360

caggagaagg cgcaggtggc cctcaccgag tatgtgcggg cgcagtaccc gtcccagccc    420

cagcgcttcg ggcgcctgct gctgcggctc cccgccctgc gcgcggtccc tgcctccctc    480

atctcccagc tgttcttcat gcgcctggtg gggaagacgc ccattgagac actgatcaga    540

gacatgctgc tgtcggggag taccttcaac tggccctacg gctcgggcca gtgaccatga    600

cggggccacg tgtgctgtgg ccaggcctgc agacagacct caagggacag ggaatgctga    660

ggcctcgagg ggcctcccgg ggcccaggac tctggcttct ctcctcagac ttctattttt    720

taaagactgt gaaatgtttg tcttttctgt tttttaaatg atcatgaaac caaaaagaga    780

ctgatcatcc aggcctcagc ctcatcctcc ccaggacccc tgtccaggat ggagggtcca    840

atcctaggac agccttgttc ctcagcaccc ctagcatgaa cttgtgggat ggtggggttg    900

gcttccctgg catgatggac aaaggcctgg cgtcggccag aggggctgct ccagtgggca    960

ggggtagcta gcgtgtgcca ggcagatcct ctggacacgt aacctatgtc agacactaca   1020

tgatgactca aggccaataa taaagacatt tcctacctgc a                       1061


<210>  4
<211>  198
<212>  PRT
<213>  Homo sapiens

<400>  4

Ala Arg Leu Leu Phe Ser Thr Val Glu Trp Ala Arg His Ala Pro Phe 
1               5                   10                  15      


Phe Pro Glu Leu Pro Val Ala Asp Gln Val Ala Leu Leu Arg Leu Ser 
            20                  25                  30          


Trp Ser Glu Leu Phe Val Leu Asn Ala Ala Gln Ala Ala Leu Pro Leu 
        35                  40                  45              


His Thr Ala Pro Leu Leu Ala Ala Ala Gly Leu His Ala Ala Pro Met 
    50                  55                  60                  


Ala Ala Glu Arg Ala Val Ala Phe Met Asp Gln Val Arg Ala Phe Gln 
65                  70                  75                  80  


Glu Gln Val Asp Lys Leu Gly Arg Leu Gln Val Asp Ser Ala Glu Tyr 
                85                  90                  95      


Gly Cys Leu Lys Ala Ile Ala Leu Phe Thr Pro Asp Ala Cys Gly Leu 
            100                 105                 110         


Ser Asp Pro Ala His Val Glu Ser Leu Gln Glu Lys Ala Gln Val Ala 
        115                 120                 125             


Leu Thr Glu Tyr Val Arg Ala Gln Tyr Pro Ser Gln Pro Gln Arg Phe 
    130                 135                 140                 


Gly Arg Leu Leu Leu Arg Leu Pro Ala Leu Arg Ala Val Pro Ala Ser 
145                 150                 155                 160 


Leu Ile Ser Gln Leu Phe Phe Met Arg Leu Val Gly Lys Thr Pro Ile 
                165                 170                 175     


Glu Thr Leu Ile Arg Asp Met Leu Leu Ser Gly Ser Thr Phe Asn Trp 
            180                 185                 190         


Pro Tyr Gly Ser Gly Gln 
        195             


<210>  5
<211>  1804
<212>  DNA
<213>  Homo Sapiens


<220>
<221>  mutation
<222>  (369)..(370)
<223>  S83A mutant of NR2F6

<400>  5
gtgcagcccg tgccccccgc gcgccggggc cgaatgcgcg ccgcgtaggg tcccccgggc     60

cgagaggggt gcccggaggg aagagcgcgg tgggggcgcc ccggccccgc tgccctgggg    120

ctatggccat ggtgaccggc ggctggggcg gccccggcgg cgacacgaac ggcgtggaca    180

aggcgggcgg ctacccgcgc gcggccgagg acgactcggc ctcgcccccc ggtgccgcca    240

gcgacgccga gccgggcgac gaggagcggc cggggctgca ggtggactgc gtggtgtgcg    300

gggacaagtc gagcggcaag cattacggtg tcttcacctg cgagggctgc aagagctttt    360

tcaagcgagc catccgccgc aacctcagct acacctgccg gtccaaccgt gactgccaga    420

tcgaccagca ccaccggaac cagtgccagt actgccgtct caagaagtgc ttccgggtgg    480

gcatgaggaa ggaggcggtg cagcgcggcc gcatcccgca ctcgctgcct ggtgccgtgg    540

ccgcctcctc gggcagcccc ccgggctcgg cgctggcggc agtggcgagc ggcggagacc    600

tcttcccggg gcagccggtg tccgaactga tcgcgcagct gctgcgcgct gagccctacc    660

ctgcggcggc cggacgcttc ggcgcagggg gcggcgcggc gggcgcggtg ctgggcatcg    720

acaacgtgtg cgagctggcg gcgcggctgc tcttcagcac cgtggagtgg gcgcgccacg    780

cgcccttctt ccccgagctg ccggtggccg accaggtggc gctgctgcgc ctgagctgga    840

gcgagctctt cgtgctgaac gcggcgcagg cggcgctgcc cctgcacacg gcgccgctac    900

tggccgccgc cggcctccac gccgcgccta tggccgccga gcgcgccgtg gctttcatgg    960

accaggtgcg cgccttccag gagcaggtgg acaagctggg ccgcctgcag gtcgactcgg   1020

ccgagtatgg ctgcctcaag gccatcgcgc tcttcacgcc cgacgcctgt ggcctctcag   1080

acccggccca cgttgagagc ctgcaggaga aggcgcaggt ggccctcacc gagtatgtgc   1140

gggcgcagta cccgtcccag ccccagcgct tcgggcgcct gctgctgcgg ctccccgccc   1200

tgcgcgcggt ccctgcctcc ctcatctccc agctgttctt catgcgcctg gtggggaaga   1260

cgcccattga gacactgatc agagacatgc tgctgtcggg gagtaccttc aactggccct   1320

acggctcggg ccagtgacca tgacggggcc acgtgtgctg tggccaggcc tgcagacaga   1380

cctcaaggga cagggaatgc tgaggcctcg aggggcctcc cggggcccag gactctggct   1440

tctctcctca gacttctatt ttttaaagac tgtgaaatgt ttgtcttttc tgttttttaa   1500

atgatcatga aaccaaaaag agactgatca tccaggcctc agcctcatcc tccccaggac   1560

ccctgtccag gatggagggt ccaatcctag gacagccttg ttcctcagca cccctagcat   1620

gaacttgtgg gatggtgggg ttggcttccc tggcatgatg gacaaaggcc tggcgtcggc   1680

cagaggggct gctccagtgg gcaggggtag ctagcgtgtg ccaggcagat cctctggaca   1740

cgtaacctat gtcagacact acatgatgac tcaaggccaa taataaagac atttcctacc   1800

tgca                                                                1804


<210>  6
<211>  404
<212>  PRT
<213>  Homo sapiens


<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  S83A mutation

<400>  6

Met Ala Met Val Thr Gly Gly Trp Gly Gly Pro Gly Gly Asp Thr Asn 
1               5                   10                  15      


Gly Val Asp Lys Ala Gly Gly Tyr Pro Arg Ala Ala Glu Asp Asp Ser 
            20                  25                  30          


Ala Ser Pro Pro Gly Ala Ala Ser Asp Ala Glu Pro Gly Asp Glu Glu 
        35                  40                  45              


Arg Pro Gly Leu Gln Val Asp Cys Val Val Cys Gly Asp Lys Ser Ser 
    50                  55                  60                  


Gly Lys His Tyr Gly Val Phe Thr Cys Glu Gly Cys Lys Ser Phe Phe 
65                  70                  75                  80  


Lys Arg Ala Ile Arg Arg Asn Leu Ser Tyr Thr Cys Arg Ser Asn Arg 
                85                  90                  95      


Asp Cys Gln Ile Asp Gln His His Arg Asn Gln Cys Gln Tyr Cys Arg 
            100                 105                 110         


Leu Lys Lys Cys Phe Arg Val Gly Met Arg Lys Glu Ala Val Gln Arg 
        115                 120                 125             


Gly Arg Ile Pro His Ser Leu Pro Gly Ala Val Ala Ala Ser Ser Gly 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ala Leu Ala Ala Val Ala Ser Gly Gly Asp Leu 
145                 150                 155                 160 


Phe Pro Gly Gln Pro Val Ser Glu Leu Ile Ala Gln Leu Leu Arg Ala 
                165                 170                 175     


Glu Pro Tyr Pro Ala Ala Ala Gly Arg Phe Gly Ala Gly Gly Gly Ala 
            180                 185                 190         


Ala Gly Ala Val Leu Gly Ile Asp Asn Val Cys Glu Leu Ala Ala Arg 
        195                 200                 205             


Leu Leu Phe Ser Thr Val Glu Trp Ala Arg His Ala Pro Phe Phe Pro 
    210                 215                 220                 


Glu Leu Pro Val Ala Asp Gln Val Ala Leu Leu Arg Leu Ser Trp Ser 
225                 230                 235                 240 


Glu Leu Phe Val Leu Asn Ala Ala Gln Ala Ala Leu Pro Leu His Thr 
                245                 250                 255     


Ala Pro Leu Leu Ala Ala Ala Gly Leu His Ala Ala Pro Met Ala Ala 
            260                 265                 270         


Glu Arg Ala Val Ala Phe Met Asp Gln Val Arg Ala Phe Gln Glu Gln 
        275                 280                 285             


Val Asp Lys Leu Gly Arg Leu Gln Val Asp Ser Ala Glu Tyr Gly Cys 
    290                 295                 300                 


Leu Lys Ala Ile Ala Leu Phe Thr Pro Asp Ala Cys Gly Leu Ser Asp 
305                 310                 315                 320 


Pro Ala His Val Glu Ser Leu Gln Glu Lys Ala Gln Val Ala Leu Thr 
                325                 330                 335     


Glu Tyr Val Arg Ala Gln Tyr Pro Ser Gln Pro Gln Arg Phe Gly Arg 
            340                 345                 350         


Leu Leu Leu Arg Leu Pro Ala Leu Arg Ala Val Pro Ala Ser Leu Ile 
        355                 360                 365             


Ser Gln Leu Phe Phe Met Arg Leu Val Gly Lys Thr Pro Ile Glu Thr 
    370                 375                 380                 


Leu Ile Arg Asp Met Leu Leu Ser Gly Ser Thr Phe Asn Trp Pro Tyr 
385                 390                 395                 400 


Gly Ser Gly Gln 
                


<210>  7
<211>  1804
<212>  DNA
<213>  Homo sapiens


<220>
<221>  mutation
<222>  (369)..(371)
<223>  S83E mutant of NR2F6

<400>  7
gtgcagcccg tgccccccgc gcgccggggc cgaatgcgcg ccgcgtaggg tcccccgggc     60

cgagaggggt gcccggaggg aagagcgcgg tgggggcgcc ccggccccgc tgccctgggg    120

ctatggccat ggtgaccggc ggctggggcg gccccggcgg cgacacgaac ggcgtggaca    180

aggcgggcgg ctacccgcgc gcggccgagg acgactcggc ctcgcccccc ggtgccgcca    240

gcgacgccga gccgggcgac gaggagcggc cggggctgca ggtggactgc gtggtgtgcg    300

gggacaagtc gagcggcaag cattacggtg tcttcacctg cgagggctgc aagagctttt    360

tcaagcgaga gatccgccgc aacctcagct acacctgccg gtccaaccgt gactgccaga    420

tcgaccagca ccaccggaac cagtgccagt actgccgtct caagaagtgc ttccgggtgg    480

gcatgaggaa ggaggcggtg cagcgcggcc gcatcccgca ctcgctgcct ggtgccgtgg    540

ccgcctcctc gggcagcccc ccgggctcgg cgctggcggc agtggcgagc ggcggagacc    600

tcttcccggg gcagccggtg tccgaactga tcgcgcagct gctgcgcgct gagccctacc    660

ctgcggcggc cggacgcttc ggcgcagggg gcggcgcggc gggcgcggtg ctgggcatcg    720

acaacgtgtg cgagctggcg gcgcggctgc tcttcagcac cgtggagtgg gcgcgccacg    780

cgcccttctt ccccgagctg ccggtggccg accaggtggc gctgctgcgc ctgagctgga    840

gcgagctctt cgtgctgaac gcggcgcagg cggcgctgcc cctgcacacg gcgccgctac    900

tggccgccgc cggcctccac gccgcgccta tggccgccga gcgcgccgtg gctttcatgg    960

accaggtgcg cgccttccag gagcaggtgg acaagctggg ccgcctgcag gtcgactcgg   1020

ccgagtatgg ctgcctcaag gccatcgcgc tcttcacgcc cgacgcctgt ggcctctcag   1080

acccggccca cgttgagagc ctgcaggaga aggcgcaggt ggccctcacc gagtatgtgc   1140

gggcgcagta cccgtcccag ccccagcgct tcgggcgcct gctgctgcgg ctccccgccc   1200

tgcgcgcggt ccctgcctcc ctcatctccc agctgttctt catgcgcctg gtggggaaga   1260

cgcccattga gacactgatc agagacatgc tgctgtcggg gagtaccttc aactggccct   1320

acggctcggg ccagtgacca tgacggggcc acgtgtgctg tggccaggcc tgcagacaga   1380

cctcaaggga cagggaatgc tgaggcctcg aggggcctcc cggggcccag gactctggct   1440

tctctcctca gacttctatt ttttaaagac tgtgaaatgt ttgtcttttc tgttttttaa   1500

atgatcatga aaccaaaaag agactgatca tccaggcctc agcctcatcc tccccaggac   1560

ccctgtccag gatggagggt ccaatcctag gacagccttg ttcctcagca cccctagcat   1620

gaacttgtgg gatggtgggg ttggcttccc tggcatgatg gacaaaggcc tggcgtcggc   1680

cagaggggct gctccagtgg gcaggggtag ctagcgtgtg ccaggcagat cctctggaca   1740

cgtaacctat gtcagacact acatgatgac tcaaggccaa taataaagac atttcctacc   1800

tgca                                                                1804


<210>  8
<211>  404
<212>  PRT
<213>  Homo sapiens


<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  S83E mutation

<400>  8

Met Ala Met Val Thr Gly Gly Trp Gly Gly Pro Gly Gly Asp Thr Asn 
1               5                   10                  15      


Gly Val Asp Lys Ala Gly Gly Tyr Pro Arg Ala Ala Glu Asp Asp Ser 
            20                  25                  30          


Ala Ser Pro Pro Gly Ala Ala Ser Asp Ala Glu Pro Gly Asp Glu Glu 
        35                  40                  45              


Arg Pro Gly Leu Gln Val Asp Cys Val Val Cys Gly Asp Lys Ser Ser 
    50                  55                  60                  


Gly Lys His Tyr Gly Val Phe Thr Cys Glu Gly Cys Lys Ser Phe Phe 
65                  70                  75                  80  


Lys Arg Glu Ile Arg Arg Asn Leu Ser Tyr Thr Cys Arg Ser Asn Arg 
                85                  90                  95      


Asp Cys Gln Ile Asp Gln His His Arg Asn Gln Cys Gln Tyr Cys Arg 
            100                 105                 110         


Leu Lys Lys Cys Phe Arg Val Gly Met Arg Lys Glu Ala Val Gln Arg 
        115                 120                 125             


Gly Arg Ile Pro His Ser Leu Pro Gly Ala Val Ala Ala Ser Ser Gly 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ala Leu Ala Ala Val Ala Ser Gly Gly Asp Leu 
145                 150                 155                 160 


Phe Pro Gly Gln Pro Val Ser Glu Leu Ile Ala Gln Leu Leu Arg Ala 
                165                 170                 175     


Glu Pro Tyr Pro Ala Ala Ala Gly Arg Phe Gly Ala Gly Gly Gly Ala 
            180                 185                 190         


Ala Gly Ala Val Leu Gly Ile Asp Asn Val Cys Glu Leu Ala Ala Arg 
        195                 200                 205             


Leu Leu Phe Ser Thr Val Glu Trp Ala Arg His Ala Pro Phe Phe Pro 
    210                 215                 220                 


Glu Leu Pro Val Ala Asp Gln Val Ala Leu Leu Arg Leu Ser Trp Ser 
225                 230                 235                 240 


Glu Leu Phe Val Leu Asn Ala Ala Gln Ala Ala Leu Pro Leu His Thr 
                245                 250                 255     


Ala Pro Leu Leu Ala Ala Ala Gly Leu His Ala Ala Pro Met Ala Ala 
            260                 265                 270         


Glu Arg Ala Val Ala Phe Met Asp Gln Val Arg Ala Phe Gln Glu Gln 
        275                 280                 285             


Val Asp Lys Leu Gly Arg Leu Gln Val Asp Ser Ala Glu Tyr Gly Cys 
    290                 295                 300                 


Leu Lys Ala Ile Ala Leu Phe Thr Pro Asp Ala Cys Gly Leu Ser Asp 
305                 310                 315                 320 


Pro Ala His Val Glu Ser Leu Gln Glu Lys Ala Gln Val Ala Leu Thr 
                325                 330                 335     


Glu Tyr Val Arg Ala Gln Tyr Pro Ser Gln Pro Gln Arg Phe Gly Arg 
            340                 345                 350         


Leu Leu Leu Arg Leu Pro Ala Leu Arg Ala Val Pro Ala Ser Leu Ile 
        355                 360                 365             


Ser Gln Leu Phe Phe Met Arg Leu Val Gly Lys Thr Pro Ile Glu Thr 
    370                 375                 380                 


Leu Ile Arg Asp Met Leu Leu Ser Gly Ser Thr Phe Asn Trp Pro Tyr 
385                 390                 395                 400 


Gly Ser Gly Gln 
                


<210>  9
<211>  1590
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: coding sequence of 
       NR2F6 wild-type Er-LBD fusion mutant"


<220>
<221>  misc_feature
<222>  (622)..(630)
<223>  linker sequence

<400>  9
atggccatgg tgaccggcgg ctggggcggc cccggcggcg acacgaacgg cgtggacaag     60

gcgggcggct acccgcgcgc ggccgaggac gactcggcct cgccccccgg tgccgccagc    120

gacgccgagc cgggcgacga ggagcggccg gggctgcagg tggactgcgt ggtgtgcggg    180

gacaagtcga gcggcaagca ttacggtgtc ttcacctgcg agggctgcaa gagctttttc    240

aagcgaagca tccgccgcaa cctcagctac acctgccggt ccaaccgtga ctgccagatc    300

gaccagcacc accggaacca gtgccagtac tgccgtctca agaagtgctt ccgggtgggc    360

atgaggaagg aggcggtgca gcgcggccgc atcccgcact cgctgcctgg tgccgtggcc    420

gcctcctcgg gcagcccccc gggctcggcg ctggcggcag tggcgagcgg cggagacctc    480

ttcccggggc agccggtgtc cgaactgatc gcgcagctgc tgcgcgctga gccctaccct    540

gcggcggccg gacgcttcgg cgcagggggc ggcgcggcgg gcgcggtgct gggcatcgac    600

aacgtgtgcg agctggcggc gtgggatcca cgaaatgaaa tgggtgcttc aggagacatg    660

agggctgcca acctttggcc aagccctctt gtgattaagc acactaagaa gaatagccct    720

gccttgtcct tgacagctga ccagatggtc agtgccttgt tggatgctga accgcccatg    780

atctattctg aatatgatcc ttctagaccc ttcagtgaag cctcaatgat gggcttattg    840

accaacctag cagataggga gctggttcat atgatcaact gggcaaagag agtgccaggc    900

tttggggact tgaatctcca tgatcaggtc caccttctcg agtgtgcctg gctggagatt    960

ctgatgattg gtctcgtctg gcgctccatg gaacacccgg ggaagctcct gtttgctcct   1020

aacttgctcc tggacaggaa tcaaggtaaa tgtgtggaag gcatggtgga gatctttgac   1080

atgttgctgg ctacgtcaag tcggttccgc atgatgaacc tgcagggaga agagtttgtg   1140

tgcctcaaat ccatcatttt gcttaattcc ggagtgtaca cgtttctgtc cagcaccttg   1200

aagtctctgg aagagaagga ccacatccac cgtgtcctgg acaagatcac agacactttg   1260

atccacctga tggccaaagc tggcctgact ctgcagcagc agcatcgccg cctagctcag   1320

ctccttctca ttctttccca tatccggcac atgagtaaca aaggcatgga gcatctctac   1380

aacatgaaat gcaagaacgt tgtgcccctc tatgacctgc tcctggagat gttggatgcc   1440

caccgccttc atgccccagc cagtcgcatg ggagtgcccc cagaggagcc cagccagacc   1500

cagctggcca ccaccagctc cacttcagca cattccttac aaacctacta catacccccg   1560

gaagcagagg gcttccccaa cacgatctga                                    1590


<210>  10
<211>  528
<212>  PRT
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: amino acid sequence of
       NR2F6 wild-type Er-LBD fusion mutant"


<220>
<221>  MISC_FEATURE
<222>  (207)..(209)
<223>  linker sequence

<400>  10

Met Ala Met Val Thr Gly Gly Trp Gly Gly Pro Gly Gly Asp Thr Asn 
1               5                   10                  15      


Gly Val Asp Lys Ala Gly Gly Tyr Pro Arg Ala Ala Glu Asp Asp Ser 
            20                  25                  30          


Ala Ser Pro Pro Gly Ala Ala Ser Asp Ala Glu Pro Gly Asp Glu Glu 
        35                  40                  45              


Arg Pro Gly Leu Gln Val Asp Cys Val Val Cys Gly Asp Lys Ser Ser 
    50                  55                  60                  


Gly Lys His Tyr Gly Val Phe Thr Cys Glu Gly Cys Lys Ser Phe Phe 
65                  70                  75                  80  


Lys Arg Ser Ile Arg Arg Asn Leu Ser Tyr Thr Cys Arg Ser Asn Arg 
                85                  90                  95      


Asp Cys Gln Ile Asp Gln His His Arg Asn Gln Cys Gln Tyr Cys Arg 
            100                 105                 110         


Leu Lys Lys Cys Phe Arg Val Gly Met Arg Lys Glu Ala Val Gln Arg 
        115                 120                 125             


Gly Arg Ile Pro His Ser Leu Pro Gly Ala Val Ala Ala Ser Ser Gly 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ala Leu Ala Ala Val Ala Ser Gly Gly Asp Leu 
145                 150                 155                 160 


Phe Pro Gly Gln Pro Val Ser Glu Leu Ile Ala Gln Leu Leu Arg Ala 
                165                 170                 175     


Glu Pro Tyr Pro Ala Ala Ala Gly Arg Phe Gly Ala Gly Gly Gly Ala 
            180                 185                 190         


Ala Gly Ala Val Leu Gly Ile Asp Asn Val Cys Glu Leu Ala Trp Asp 
        195                 200                 205             


Pro Arg Asn Glu Met Gly Ala Ser Gly Asp Met Arg Ala Ala Asn Leu 
    210                 215                 220                 


Trp Pro Ser Pro Leu Val Ile Lys His Thr Lys Lys Asn Ser Pro Ala 
225                 230                 235                 240 


Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu Asp Ala Glu 
                245                 250                 255     


Pro Pro Met Ile Tyr Ser Glu Tyr Asp Pro Ser Arg Pro Phe Ser Glu 
            260                 265                 270         


Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg Glu Leu Val 
        275                 280                 285             


His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Gly Asp Leu Asn 
    290                 295                 300                 


Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu Glu Ile Leu 
305                 310                 315                 320 


Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly Lys Leu Leu 
                325                 330                 335     


Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys Cys Val Glu 
            340                 345                 350         


Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser Ser Arg Phe 
        355                 360                 365             


Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu Lys Ser Ile 
    370                 375                 380                 


Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys 
385                 390                 395                 400 


Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp Lys Ile Thr 
                405                 410                 415     


Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr Leu Gln Gln 
            420                 425                 430         


Gln His Arg Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser His Ile Arg 
        435                 440                 445             


His Met Ser Asn Lys Gly Met Glu His Leu Tyr Asn Met Lys Cys Lys 
    450                 455                 460                 


Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu Asp Ala His 
465                 470                 475                 480 


Arg Leu His Ala Pro Ala Ser Arg Met Gly Val Pro Pro Glu Glu Pro 
                485                 490                 495     


Ser Gln Thr Gln Leu Ala Thr Thr Ser Ser Thr Ser Ala His Ser Leu 
            500                 505                 510         


Gln Thr Tyr Tyr Ile Pro Pro Glu Ala Glu Gly Phe Pro Asn Thr Ile 
        515                 520                 525             


<210>  11
<211>  1590
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: coding sequence of 
       S83A mutated NR2F6 ER-LBD fusion mutant"


<220>
<221>  mutation
<222>  (247)..(248)

<220>
<221>  misc_feature
<222>  (622)..(630)
<223>  linker sequence

<400>  11
atggccatgg tgaccggcgg ctggggcggc cccggcggcg acacgaacgg cgtggacaag     60

gcgggcggct acccgcgcgc ggccgaggac gactcggcct cgccccccgg tgccgccagc    120

gacgccgagc cgggcgacga ggagcggccg gggctgcagg tggactgcgt ggtgtgcggg    180

gacaagtcga gcggcaagca ttacggtgtc ttcacctgcg agggctgcaa gagctttttc    240

aagcgagcca tccgccgcaa cctcagctac acctgccggt ccaaccgtga ctgccagatc    300

gaccagcacc accggaacca gtgccagtac tgccgtctca agaagtgctt ccgggtgggc    360

atgaggaagg aggcggtgca gcgcggccgc atcccgcact cgctgcctgg tgccgtggcc    420

gcctcctcgg gcagcccccc gggctcggcg ctggcggcag tggcgagcgg cggagacctc    480

ttcccggggc agccggtgtc cgaactgatc gcgcagctgc tgcgcgctga gccctaccct    540

gcggcggccg gacgcttcgg cgcagggggc ggcgcggcgg gcgcggtgct gggcatcgac    600

aacgtgtgcg agctggcggc gtgggatcca cgaaatgaaa tgggtgcttc aggagacatg    660

agggctgcca acctttggcc aagccctctt gtgattaagc acactaagaa gaatagccct    720

gccttgtcct tgacagctga ccagatggtc agtgccttgt tggatgctga accgcccatg    780

atctattctg aatatgatcc ttctagaccc ttcagtgaag cctcaatgat gggcttattg    840

accaacctag cagataggga gctggttcat atgatcaact gggcaaagag agtgccaggc    900

tttggggact tgaatctcca tgatcaggtc caccttctcg agtgtgcctg gctggagatt    960

ctgatgattg gtctcgtctg gcgctccatg gaacacccgg ggaagctcct gtttgctcct   1020

aacttgctcc tggacaggaa tcaaggtaaa tgtgtggaag gcatggtgga gatctttgac   1080

atgttgctgg ctacgtcaag tcggttccgc atgatgaacc tgcagggaga agagtttgtg   1140

tgcctcaaat ccatcatttt gcttaattcc ggagtgtaca cgtttctgtc cagcaccttg   1200

aagtctctgg aagagaagga ccacatccac cgtgtcctgg acaagatcac agacactttg   1260

atccacctga tggccaaagc tggcctgact ctgcagcagc agcatcgccg cctagctcag   1320

ctccttctca ttctttccca tatccggcac atgagtaaca aaggcatgga gcatctctac   1380

aacatgaaat gcaagaacgt tgtgcccctc tatgacctgc tcctggagat gttggatgcc   1440

caccgccttc atgccccagc cagtcgcatg ggagtgcccc cagaggagcc cagccagacc   1500

cagctggcca ccaccagctc cacttcagca cattccttac aaacctacta catacccccg   1560

gaagcagagg gcttccccaa cacgatctga                                    1590


<210>  12
<211>  528
<212>  PRT
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: amino acid sequence of
       coding sequence of S83A mutated NR2F6 ER-LBD fusion mutant"


<220>
<221>  VARIANT
<222>  (83)..(83)

<220>
<221>  MISC_FEATURE
<222>  (207)..(209)
<223>  linker sequence

<400>  12

Met Ala Met Val Thr Gly Gly Trp Gly Gly Pro Gly Gly Asp Thr Asn 
1               5                   10                  15      


Gly Val Asp Lys Ala Gly Gly Tyr Pro Arg Ala Ala Glu Asp Asp Ser 
            20                  25                  30          


Ala Ser Pro Pro Gly Ala Ala Ser Asp Ala Glu Pro Gly Asp Glu Glu 
        35                  40                  45              


Arg Pro Gly Leu Gln Val Asp Cys Val Val Cys Gly Asp Lys Ser Ser 
    50                  55                  60                  


Gly Lys His Tyr Gly Val Phe Thr Cys Glu Gly Cys Lys Ser Phe Phe 
65                  70                  75                  80  


Lys Arg Ala Ile Arg Arg Asn Leu Ser Tyr Thr Cys Arg Ser Asn Arg 
                85                  90                  95      


Asp Cys Gln Ile Asp Gln His His Arg Asn Gln Cys Gln Tyr Cys Arg 
            100                 105                 110         


Leu Lys Lys Cys Phe Arg Val Gly Met Arg Lys Glu Ala Val Gln Arg 
        115                 120                 125             


Gly Arg Ile Pro His Ser Leu Pro Gly Ala Val Ala Ala Ser Ser Gly 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ala Leu Ala Ala Val Ala Ser Gly Gly Asp Leu 
145                 150                 155                 160 


Phe Pro Gly Gln Pro Val Ser Glu Leu Ile Ala Gln Leu Leu Arg Ala 
                165                 170                 175     


Glu Pro Tyr Pro Ala Ala Ala Gly Arg Phe Gly Ala Gly Gly Gly Ala 
            180                 185                 190         


Ala Gly Ala Val Leu Gly Ile Asp Asn Val Cys Glu Leu Ala Trp Asp 
        195                 200                 205             


Pro Arg Asn Glu Met Gly Ala Ser Gly Asp Met Arg Ala Ala Asn Leu 
    210                 215                 220                 


Trp Pro Ser Pro Leu Val Ile Lys His Thr Lys Lys Asn Ser Pro Ala 
225                 230                 235                 240 


Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu Asp Ala Glu 
                245                 250                 255     


Pro Pro Met Ile Tyr Ser Glu Tyr Asp Pro Ser Arg Pro Phe Ser Glu 
            260                 265                 270         


Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg Glu Leu Val 
        275                 280                 285             


His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Gly Asp Leu Asn 
    290                 295                 300                 


Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu Glu Ile Leu 
305                 310                 315                 320 


Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly Lys Leu Leu 
                325                 330                 335     


Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys Cys Val Glu 
            340                 345                 350         


Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser Ser Arg Phe 
        355                 360                 365             


Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu Lys Ser Ile 
    370                 375                 380                 


Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys 
385                 390                 395                 400 


Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp Lys Ile Thr 
                405                 410                 415     


Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr Leu Gln Gln 
            420                 425                 430         


Gln His Arg Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser His Ile Arg 
        435                 440                 445             


His Met Ser Asn Lys Gly Met Glu His Leu Tyr Asn Met Lys Cys Lys 
    450                 455                 460                 


Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu Asp Ala His 
465                 470                 475                 480 


Arg Leu His Ala Pro Ala Ser Arg Met Gly Val Pro Pro Glu Glu Pro 
                485                 490                 495     


Ser Gln Thr Gln Leu Ala Thr Thr Ser Ser Thr Ser Ala His Ser Leu 
            500                 505                 510         


Gln Thr Tyr Tyr Ile Pro Pro Glu Ala Glu Gly Phe Pro Asn Thr Ile 
        515                 520                 525             


<210>  13
<211>  1590
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: coding sequence of 
       S83E mutated NR2F6 ER-LBD fusion mutant"


<220>
<221>  mutation
<222>  (247)..(249)
<223>  S83E

<220>
<221>  misc_feature
<222>  (622)..(630)
<223>  linker sequence

<400>  13
atggccatgg tgaccggcgg ctggggcggc cccggcggcg acacgaacgg cgtggacaag     60

gcgggcggct acccgcgcgc ggccgaggac gactcggcct cgccccccgg tgccgccagc    120

gacgccgagc cgggcgacga ggagcggccg gggctgcagg tggactgcgt ggtgtgcggg    180

gacaagtcga gcggcaagca ttacggtgtc ttcacctgcg agggctgcaa gagctttttc    240

aagcgagaga tccgccgcaa cctcagctac acctgccggt ccaaccgtga ctgccagatc    300

gaccagcacc accggaacca gtgccagtac tgccgtctca agaagtgctt ccgggtgggc    360

atgaggaagg aggcggtgca gcgcggccgc atcccgcact cgctgcctgg tgccgtggcc    420

gcctcctcgg gcagcccccc gggctcggcg ctggcggcag tggcgagcgg cggagacctc    480

ttcccggggc agccggtgtc cgaactgatc gcgcagctgc tgcgcgctga gccctaccct    540

gcggcggccg gacgcttcgg cgcagggggc ggcgcggcgg gcgcggtgct gggcatcgac    600

aacgtgtgcg agctggcggc gtgggatcca cgaaatgaaa tgggtgcttc aggagacatg    660

agggctgcca acctttggcc aagccctctt gtgattaagc acactaagaa gaatagccct    720

gccttgtcct tgacagctga ccagatggtc agtgccttgt tggatgctga accgcccatg    780

atctattctg aatatgatcc ttctagaccc ttcagtgaag cctcaatgat gggcttattg    840

accaacctag cagataggga gctggttcat atgatcaact gggcaaagag agtgccaggc    900

tttggggact tgaatctcca tgatcaggtc caccttctcg agtgtgcctg gctggagatt    960

ctgatgattg gtctcgtctg gcgctccatg gaacacccgg ggaagctcct gtttgctcct   1020

aacttgctcc tggacaggaa tcaaggtaaa tgtgtggaag gcatggtgga gatctttgac   1080

atgttgctgg ctacgtcaag tcggttccgc atgatgaacc tgcagggaga agagtttgtg   1140

tgcctcaaat ccatcatttt gcttaattcc ggagtgtaca cgtttctgtc cagcaccttg   1200

aagtctctgg aagagaagga ccacatccac cgtgtcctgg acaagatcac agacactttg   1260

atccacctga tggccaaagc tggcctgact ctgcagcagc agcatcgccg cctagctcag   1320

ctccttctca ttctttccca tatccggcac atgagtaaca aaggcatgga gcatctctac   1380

aacatgaaat gcaagaacgt tgtgcccctc tatgacctgc tcctggagat gttggatgcc   1440

caccgccttc atgccccagc cagtcgcatg ggagtgcccc cagaggagcc cagccagacc   1500

cagctggcca ccaccagctc cacttcagca cattccttac aaacctacta catacccccg   1560

gaagcagagg gcttccccaa cacgatctga                                    1590


<210>  14
<211>  528
<212>  PRT
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: binding buffer: 
       double-stranded oligonucelotide probes"


<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  S83E

<220>
<221>  MISC_FEATURE
<222>  (207)..(209)
<223>  linker sequence

<400>  14

Met Ala Met Val Thr Gly Gly Trp Gly Gly Pro Gly Gly Asp Thr Asn 
1               5                   10                  15      


Gly Val Asp Lys Ala Gly Gly Tyr Pro Arg Ala Ala Glu Asp Asp Ser 
            20                  25                  30          


Ala Ser Pro Pro Gly Ala Ala Ser Asp Ala Glu Pro Gly Asp Glu Glu 
        35                  40                  45              


Arg Pro Gly Leu Gln Val Asp Cys Val Val Cys Gly Asp Lys Ser Ser 
    50                  55                  60                  


Gly Lys His Tyr Gly Val Phe Thr Cys Glu Gly Cys Lys Ser Phe Phe 
65                  70                  75                  80  


Lys Arg Glu Ile Arg Arg Asn Leu Ser Tyr Thr Cys Arg Ser Asn Arg 
                85                  90                  95      


Asp Cys Gln Ile Asp Gln His His Arg Asn Gln Cys Gln Tyr Cys Arg 
            100                 105                 110         


Leu Lys Lys Cys Phe Arg Val Gly Met Arg Lys Glu Ala Val Gln Arg 
        115                 120                 125             


Gly Arg Ile Pro His Ser Leu Pro Gly Ala Val Ala Ala Ser Ser Gly 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ala Leu Ala Ala Val Ala Ser Gly Gly Asp Leu 
145                 150                 155                 160 


Phe Pro Gly Gln Pro Val Ser Glu Leu Ile Ala Gln Leu Leu Arg Ala 
                165                 170                 175     


Glu Pro Tyr Pro Ala Ala Ala Gly Arg Phe Gly Ala Gly Gly Gly Ala 
            180                 185                 190         


Ala Gly Ala Val Leu Gly Ile Asp Asn Val Cys Glu Leu Ala Trp Asp 
        195                 200                 205             


Pro Arg Asn Glu Met Gly Ala Ser Gly Asp Met Arg Ala Ala Asn Leu 
    210                 215                 220                 


Trp Pro Ser Pro Leu Val Ile Lys His Thr Lys Lys Asn Ser Pro Ala 
225                 230                 235                 240 


Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu Asp Ala Glu 
                245                 250                 255     


Pro Pro Met Ile Tyr Ser Glu Tyr Asp Pro Ser Arg Pro Phe Ser Glu 
            260                 265                 270         


Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg Glu Leu Val 
        275                 280                 285             


His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Gly Asp Leu Asn 
    290                 295                 300                 


Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu Glu Ile Leu 
305                 310                 315                 320 


Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly Lys Leu Leu 
                325                 330                 335     


Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys Cys Val Glu 
            340                 345                 350         


Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser Ser Arg Phe 
        355                 360                 365             


Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu Lys Ser Ile 
    370                 375                 380                 


Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys 
385                 390                 395                 400 


Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp Lys Ile Thr 
                405                 410                 415     


Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr Leu Gln Gln 
            420                 425                 430         


Gln His Arg Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser His Ile Arg 
        435                 440                 445             


His Met Ser Asn Lys Gly Met Glu His Leu Tyr Asn Met Lys Cys Lys 
    450                 455                 460                 


Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu Asp Ala His 
465                 470                 475                 480 


Arg Leu His Ala Pro Ala Ser Arg Met Gly Val Pro Pro Glu Glu Pro 
                485                 490                 495     


Ser Gln Thr Gln Leu Ala Thr Thr Ser Ser Thr Ser Ala His Ser Leu 
            500                 505                 510         


Gln Thr Tyr Tyr Ile Pro Pro Glu Ala Glu Gly Phe Pro Asn Thr Ile 
        515                 520                 525             


<210>  15
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: binding buffer: 
       double-stranded oligonucelotide probes"

<400>  15
gccatggggg gatccccgaa gtcc                                            24


<210>  16
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: binding buffer: 
       double-stranded oligonucelotide probes"

<400>  16
cgcttgatga ctcagccgga a                                               21


<210>  17
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: binding buffer: 
       double-stranded oligonucelotide probes"

<400>  17
gcccaaagag gaaaatttgt ttcatacag                                       29


<210>  18
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: binding buffer: 
       double-stranded oligonucelotide probes"

<400>  18
gtgtcaaagg tcgtgtcaaa ggtc                                            24


<210>  19
<211>  21
<212>  RNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: siGenome ON-TARGETplus
       SMARTpool duplex (9), J-045088-09,NR2F6, sense sequence"

<400>  19
guggaaagca uuacggcguu u                                               21


<210>  20
<211>  21
<212>  RNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: siGenome ON-TARGETplus
       SMARTpool duplex (9), J-045088-09,NR2F6, antisense sequence"

<400>  20
acgccguaau gcuuuccacu u                                               21


<210>  21
<211>  21
<212>  RNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: siGenome ON-TARGETplus
       SMARTpool duplex (10), J-045088-10, NR2F6, sense sequence"

<400>  21
agguggaugc ugcggaguau u                                                 21


<210>  22
<211>  21
<212>  RNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: siGenome ON-TARGETplus
       SMARTpool duplex (10), J-045088-10,NR2F6, antisense sequence"

<400>  22
uacuccgcag cauccaccuu u                                               21


<210>  23
<211>  21
<212>  RNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: siGenome ON-TARGETplus
       SMARTpool duplex (11), J-045088-11, NR2F6, sense sequence"

<400>  23
gcaucgacaa cgugugcgau u                                               21


<210>  24
<211>  21
<212>  RNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: siGenome ON-TARGETplus
       SMARTpool duplex (11), J-045088-11,NR2F6, antisense sequence"

<400>  24
ucgcacacgu ugucgaugcu u                                               21


<210>  25
<211>  21
<212>  RNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: siGenome ON-TARGETplus
       SMARTpool duplex (12), J-045088-12,NR2F6, sense sequence"

<400>  25
ggcaagacac ccaucgagau u                                               21


<210>  26
<211>  21
<212>  RNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: siGenome ON-TARGETplus
       SMARTpool duplex (12), J-045088-12, NR2F6, antisense sequence"

<400>  26
ucucgauggg ugucuugccu u                                               21

210>   27
<211>  4044
<212>  DNA
<213>  pRL-TK RENILLA Vector sequence

<400>  27
agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct       60

gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt      120

cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc      180

cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc      240

gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg      300

gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg      360

gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac      420

gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat      480

cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat      540

ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt      600

ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac      660

gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt      720

ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca      780

acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag      840

gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc      900

ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca      960

ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta     1020

taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac     1080

tggtccgcag tggtgggcca atgtaaacaa atgaatgttc ttgattcatt tattaattat     1140

tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct     1200

tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca     1260

gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag gttacttgat     1320

cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt     1380

gtcggccatg attggggtgc ttgtttggca tttcattata gctatgagca tcaagataag     1440

atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg     1500

cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa aatggttttg     1560

gagaataact tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca     1620

gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca     1680

ttatcatggc ctcgtgaaat cccgttagta aaaggtggta aacctgacgt tgtacaaatt     1740

gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat gtttattgaa     1800

tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt tcctaatact     1860

gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga     1920

aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctagagcggc     1980

cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc     2040

agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta     2100

taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg     2160

gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata     2220

aggatccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct     2280

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     2340

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg     2400

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg     2460

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc     2520

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat     2580

gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact     2640

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca     2700

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact     2760

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg     2820

atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg     2880

agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg     2940

aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg     3000

caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag     3060

ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc     3120

gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga     3180

tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat     3240

atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc     3300

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     3360

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     3420

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     3480

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc     3540

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     3600

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     3660

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     3720

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     3780

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     3840

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     3900

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     3960

ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct     4020

ggccttttgc tcacatggct cgac                                            4044


<210>  28
<211>  29
<212>  DNA
<213>  NF-AT/AP-1 Consensus Oligonucleotide from IL-2 distal site 
       (3 repeats)

<400>  28
gcccaaagag gaaaatttgt ttcatacag                                         29


<210>  29
<211>  1159
<212>  DNA
<213>  Homo sapiens interleukin17 (IL17) gene, exon 1 promoter
       region and partial cds

<400>  29
ggatctcagg acaaacaggt tcaggatccg gcaaactaat tttacactcc agccattgag       60

ttggaactac tggccagcct ccccccgagt tagcatgtag aatatgggat accagctgag      120

tgcctgagag ttatcattca cctcagtggg ggtaggggcg gagaagggtg acatatagcc      180

agccacatct atatccactg gcccttcctt gtcctagtcc tctgtattcc tgagaaggaa      240

ctattctcaa ggacctgagt ccaagttcat cttacttaga gtacagagaa aagaaccgct      300

aactccttct ctctttcccc catcatgtct cctctccttt ctagttctca tcactctcta      360

ctcccccctg cccccctttt ctccatctcc atcacctttg tccagtctct atccccattt      420

tcaattcctt cctcaaaaca ccaagttgct tggtagcatg cagggttgga acatgccttt      480

aacagaaaat ctcgtgtctc ttgaacctag ttatttattc cttgagcaga gtagatattc      540

aacaaaagaa ttgttaaatt caattaaata ggatatatct tattattaaa tatttttttc      600

attttttgtt tacttatatg atgggaactt gagtagtttc cggaattgtc tccacaacac      660

ctggccaagg aatctgtgag gaaaagaaag atcaaatgga aaatcaaggt acatgacacc      720

agaagaccta catgttactt caaacttttt cttcctcatg aaccattaaa atagagcata      780

actcttctgg cagctgtaca tatgttcata aatacatgat attgacccat agcatagcag      840

ctctgctcag cttctaacaa gtaagaatga aaagaggaca tggtctttag gaacatgaat      900

ttctgccctt cccattttcc ttcagaagga gagattcttc tatgacctca ttgggggcgg      960

aaattttaac caaaatggtg tcacccctga acccactgcg acacgccacg taagtgacca     1020

cagaaggaga aaagccctat aaaaagagag acgatagcgc tacattttgt ccatctcata     1080

gcaggcacaa actcatccat ccccagttga ttggaagaaa caacgatgac tcctgggaag     1140

acctcattgg tggtgagtc                                                  1159


<210>  30
<211>  1804
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: coding sequence 
       of C112S mutated NR2F6"

<220>
<221>  mutation
<222>  (451)..(453)
<223>  C112S

<400>  30
gtgcagcccg tgccccccgc gcgccggggc cgaatgcgcg ccgcgtaggg tcccccgggc       60

cgagaggggt gcccggaggg aagagcgcgg tgggggcgcc ccggccccgc tgccctgggg      120

ctatggccat ggtgaccggc ggctggggcg gccccggcgg cgacacgaac ggcgtggaca      180

aggcgggcgg ctacccgcgc gcggccgagg acgactcggc ctcgcccccc ggtgccgcca      240

gcgacgccga gccgggcgac gaggagcggc cggggctgca ggtggactgc gtggtgtgcg      300

gggacaagtc gagcggcaag cattacggtg tcttcacctg cgagggctgc aagagctttt      360

tcaagcgaag catccgccgc aacctcagct acacctgccg gtccaaccgt gactgccaga      420

tcgaccagca ccaccggaac cagtgccagt ctggccgtct caagaagtgc ttccgggtgg      480

gcatgaggaa ggaggcggtg cagcgcggcc gcatcccgca ctcgctgcct ggtgccgtgg      540

ccgcctcctc gggcagcccc ccgggctcgg cgctggcggc agtggcgagc ggcggagacc      600

tcttcccggg gcagccggtg tccgaactga tcgcgcagct gctgcgcgct gagccctacc      660

ctgcggcggc cggacgcttc ggcgcagggg gcggcgcggc gggcgcggtg ctgggcatcg      720

acaacgtgtg cgagctggcg gcgcggctgc tcttcagcac cgtggagtgg gcgcgccacg      780

cgcccttctt ccccgagctg ccggtggccg accaggtggc gctgctgcgc ctgagctgga      840

gcgagctctt cgtgctgaac gcggcgcagg cggcgctgcc cctgcacacg gcgccgctac      900

tggccgccgc cggcctccac gccgcgccta tggccgccga gcgcgccgtg gctttcatgg      960

accaggtgcg cgccttccag gagcaggtgg acaagctggg ccgcctgcag gtcgactcgg     1020

ccgagtatgg ctgcctcaag gccatcgcgc tcttcacgcc cgacgcctgt ggcctctcag     1080

acccggccca cgttgagagc ctgcaggaga aggcgcaggt ggccctcacc gagtatgtgc     1140

gggcgcagta cccgtcccag ccccagcgct tcgggcgcct gctgctgcgg ctccccgccc     1200

tgcgcgcggt ccctgcctcc ctcatctccc agctgttctt catgcgcctg gtggggaaga     1260

cgcccattga gacactgatc agagacatgc tgctgtcggg gagtaccttc aactggccct     1320

acggctcggg ccagtgacca tgacggggcc acgtgtgctg tggccaggcc tgcagacaga     1380

cctcaaggga cagggaatgc tgaggcctcg aggggcctcc cggggcccag gactctggct     1440

tctctcctca gacttctatt ttttaaagac tgtgaaatgt ttgtcttttc tgttttttaa     1500

atgatcatga aaccaaaaag agactgatca tccaggcctc agcctcatcc tccccaggac     1560

ccctgtccag gatggagggt ccaatcctag gacagccttg ttcctcagca cccctagcat     1620

gaacttgtgg gatggtgggg ttggcttccc tggcatgatg gacaaaggcc tggcgtcggc     1680

cagaggggct gctccagtgg gcaggggtag ctagcgtgtg ccaggcagat cctctggaca     1740

cgtaacctat gtcagacact acatgatgac tcaaggccaa taataaagac atttcctacc     1800

tgca                                                                  1804


<210>  31
<211>  404
<212>  PRT
<213>  artificial sequence

<220>
<221>  VARIANT
<222>  (111)..(111)


<400>  31

Met Ala Met Val Thr Gly Gly Trp Gly Gly Pro Gly Gly Asp Thr Asn 
1               5                   10                  15      


Gly Val Asp Lys Ala Gly Gly Tyr Pro Arg Ala Ala Glu Asp Asp Ser 
            20                  25                  30          


Ala Ser Pro Pro Gly Ala Ala Ser Asp Ala Glu Pro Gly Asp Glu Glu 
        35                  40                  45              


Arg Pro Gly Leu Gln Val Asp Cys Val Val Cys Gly Asp Lys Ser Ser 
    50                  55                  60                  


Gly Lys His Tyr Gly Val Phe Thr Cys Glu Gly Cys Lys Ser Phe Phe 
65                  70                  75                  80  


Lys Arg Ser Ile Arg Arg Asn Leu Ser Tyr Thr Cys Arg Ser Asn Arg 
                85                  90                  95      


Asp Cys Gln Ile Asp Gln His His Arg Asn Gln Cys Gln Tyr Ser Arg 
            100                 105                 110         


Leu Lys Lys Cys Phe Arg Val Gly Met Arg Lys Glu Ala Val Gln Arg 
        115                 120                 125             


Gly Arg Ile Pro His Ser Leu Pro Gly Ala Val Ala Ala Ser Ser Gly 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ala Leu Ala Ala Val Ala Ser Gly Gly Asp Leu 
145                 150                 155                 160 


Phe Pro Gly Gln Pro Val Ser Glu Leu Ile Ala Gln Leu Leu Arg Ala 
                165                 170                 175     


Glu Pro Tyr Pro Ala Ala Ala Gly Arg Phe Gly Ala Gly Gly Gly Ala 
            180                 185                 190         


Ala Gly Ala Val Leu Gly Ile Asp Asn Val Cys Glu Leu Ala Ala Arg 
        195                 200                 205             


Leu Leu Phe Ser Thr Val Glu Trp Ala Arg His Ala Pro Phe Phe Pro 
    210                 215                 220                 


Glu Leu Pro Val Ala Asp Gln Val Ala Leu Leu Arg Leu Ser Trp Ser 
225                 230                 235                 240 


Glu Leu Phe Val Leu Asn Ala Ala Gln Ala Ala Leu Pro Leu His Thr 
                245                 250                 255     


Ala Pro Leu Leu Ala Ala Ala Gly Leu His Ala Ala Pro Met Ala Ala 
            260                 265                 270         


Glu Arg Ala Val Ala Phe Met Asp Gln Val Arg Ala Phe Gln Glu Gln 
        275                 280                 285             


Val Asp Lys Leu Gly Arg Leu Gln Val Asp Ser Ala Glu Tyr Gly Cys 
    290                 295                 300                 


Leu Lys Ala Ile Ala Leu Phe Thr Pro Asp Ala Cys Gly Leu Ser Asp 
305                 310                 315                 320 


Pro Ala His Val Glu Ser Leu Gln Glu Lys Ala Gln Val Ala Leu Thr 
                325                 330                 335     


Glu Tyr Val Arg Ala Gln Tyr Pro Ser Gln Pro Gln Arg Phe Gly Arg 
            340                 345                 350         


Leu Leu Leu Arg Leu Pro Ala Leu Arg Ala Val Pro Ala Ser Leu Ile 
        355                 360                 365             


Ser Gln Leu Phe Phe Met Arg Leu Val Gly Lys Thr Pro Ile Glu Thr 
    370                 375                 380                 


Leu Ile Arg Asp Met Leu Leu Ser Gly Ser Thr Phe Asn Trp Pro Tyr 
385                 390                 395                 400 


Gly Ser Gly Gln 
                


<210>  32
<211>  1653
<212>  DNA
<213>  Photinus pyralis (common eastern firefly luciferase)

<400>  32
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt      360

tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga      600

tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg      660

catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt      720

gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt      780

cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac      840

aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg      900

attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg      960

aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat     1020

gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc     1080

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa     1140

acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt     1200

tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct     1260

ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct     1320

ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa     1380

caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt     1440

cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat     1500

tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac     1560

gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata     1620

aaggccaaga agggcggaaa gtccaaattg taa                                  1653


<210>  33
<211>  550
<212>  PRT
<213>   Photinus pyralis (common eastern firefly luciferase)

<400>  33

Met Glu Asp Ala Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro 
1               5                   10                  15      


Leu Glu Asp Gly Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg 
            20                  25                  30          


Tyr Ala Leu Val Pro Gly Thr Ile Ala Phe Thr Asp Ala His Ile Glu 
        35                  40                  45              


Val Asn Ile Thr Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala 
    50                  55                  60                  


Glu Ala Met Lys Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val 
65                  70                  75                  80  


Cys Ser Glu Asn Ser Leu Gln Phe Phe Met Pro Val Leu Gly Ala Leu 
                85                  90                  95      


Phe Ile Gly Val Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg 
            100                 105                 110         


Glu Leu Leu Asn Ser Met Asn Ile Ser Gln Pro Thr Val Val Phe Val 
        115                 120                 125             


Ser Lys Lys Gly Leu Gln Lys Ile Leu Asn Val Gln Lys Lys Leu Pro 
    130                 135                 140                 


Ile Ile Gln Lys Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly 
145                 150                 155                 160 


Phe Gln Ser Met Tyr Thr Phe Val Thr Ser His Leu Pro Pro Gly Phe 
                165                 170                 175     


Asn Glu Tyr Asp Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile 
            180                 185                 190         


Ala Leu Ile Met Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val 
        195                 200                 205             


Ala Leu Pro His Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp 
    210                 215                 220                 


Pro Ile Phe Gly Asn Gln Ile Ile Pro Asp Thr Ala Ile Leu Ser Val 
225                 230                 235                 240 


Val Pro Phe His His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu 
                245                 250                 255     


Ile Cys Gly Phe Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu 
            260                 265                 270         


Phe Leu Arg Ser Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val 
        275                 280                 285             


Pro Thr Leu Phe Ser Phe Phe Ala Lys Ser Thr Leu Ile Asp Lys Tyr 
    290                 295                 300                 


Asp Leu Ser Asn Leu His Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser 
305                 310                 315                 320 


Lys Glu Val Gly Glu Ala Val Ala Lys Arg Phe His Leu Pro Gly Ile 
                325                 330                 335     


Arg Gln Gly Tyr Gly Leu Thr Glu Thr Thr Ser Ala Ile Leu Ile Thr 
            340                 345                 350         


Pro Glu Gly Asp Asp Lys Pro Gly Ala Val Gly Lys Val Val Pro Phe 
        355                 360                 365             


Phe Glu Ala Lys Val Val Asp Leu Asp Thr Gly Lys Thr Leu Gly Val 
    370                 375                 380                 


Asn Gln Arg Gly Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly 
385                 390                 395                 400 


Tyr Val Asn Asn Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly 
                405                 410                 415     


Trp Leu His Ser Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe 
            420                 425                 430         


Phe Ile Val Asp Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln 
        435                 440                 445             


Val Ala Pro Ala Glu Leu Glu Ser Ile Leu Leu Gln His Pro Asn Ile 
    450                 455                 460                 


Phe Asp Ala Gly Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu 
465                 470                 475                 480 


Pro Ala Ala Val Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys 
                485                 490                 495     


Glu Ile Val Asp Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu 
            500                 505                 510         


Arg Gly Gly Val Val Phe Val Asp Glu Val Pro Lys Gly Leu Thr Gly 
        515                 520                 525             


Lys Leu Asp Ala Arg Lys Ile Arg Glu Ile Leu Ile Lys Ala Lys Lys 
    530                 535                 540                 


Gly Gly Lys Ser Lys Leu 
545                 550 





