                         SEQUENCE LISTING

<110>  UNIVERSITE JOSEPH FOURIER
       INSTITUT PASTEUR
 
<120>  MUTATIONS DANS LA PROTEINE NS5B DU VHC

<130>  U19-B-27179 PCT

<150>  FR08.54292
<151>  2008-06-26

<160>  8     

<170>  PatentIn version 3.3

<210>  1
<211>  591
<212>  PRT
<213>  artificial sequence

<220>
<223>  Rfrence NS5B

<400>  1

Ser Met Ser Tyr Thr Trp Thr Gly Ala Leu Ile Thr Pro Cys Ala Ala 
1               5                   10                  15      


Glu Glu Ser Lys Leu Pro Ile Asn Ala Leu Ser Asn Ser Leu Leu Arg 
            20                  25                  30          


His His Asn Met Val Tyr Ala Thr Thr Ser Arg Ser Ala Ser Gln Arg 
        35                  40                  45              


Gln Lys Lys Val Thr Phe Asp Arg Leu Gln Val Leu Asp Asn His Tyr 
    50                  55                  60                  


Gln Asp Val Leu Lys Glu Met Lys Ala Lys Ala Ser Thr Val Lys Ala 
65                  70                  75                  80  


Arg Leu Leu Ser Val Glu Glu Ala Cys Lys Leu Thr Pro Pro His Ser 
                85                  90                  95      


Ala Lys Ser Lys Phe Gly Tyr Gly Ala Lys Asp Val Arg Asn Leu Ser 
            100                 105                 110         


Ser Lys Ala Val Asn His Ile Arg Ser Val Trp Glu Asp Leu Leu Glu 
        115                 120                 125             


Asp Thr Asp Thr Pro Ile Asp Thr Thr Ile Met Ala Lys Asn Glu Val 
    130                 135                 140                 


Phe Cys Val Gln Pro Glu Lys Gly Gly Arg Lys Pro Ala Arg Leu Ile 
145                 150                 155                 160 


Val Phe Pro Asp Leu Gly Val Arg Val Cys Glu Lys Met Ala Leu Tyr 
                165                 170                 175     


Asp Val Val Ser Thr Leu Pro Gln Ala Val Met Gly Ser Ala Tyr Gly 
            180                 185                 190         


Phe Gln Tyr Ser Pro Gly Gln Arg Val Glu Phe Leu Val Asn Ala Trp 
        195                 200                 205             


Lys Ser Lys Lys Thr Pro Met Gly Phe Ala Tyr Asp Thr Arg Cys Phe 
    210                 215                 220                 


Asp Ser Thr Val Thr Glu Ser Asp Ile Arg Val Glu Glu Ser Ile Tyr 
225                 230                 235                 240 


Gln Cys Cys Asp Leu Ala Pro Glu Ala Arg Gln Ala Ile Arg Ser Leu 
                245                 250                 255     


Thr Glu Arg Leu Tyr Val Gly Gly Pro Leu Thr Asn Ser Lys Gly Gln 
            260                 265                 270         


Asn Cys Gly Tyr Arg Arg Cys Arg Ala Ser Gly Val Leu Thr Thr Ser 
        275                 280                 285             


Cys Gly Asn Thr Leu Thr Cys Tyr Leu Lys Ala Ser Ala Ala Cys Arg 
    290                 295                 300                 


Ala Ala Lys Leu Gln Asp Cys Thr Met Leu Val Asn Gly Asp Asp Leu 
305                 310                 315                 320 


Val Val Ile Cys Glu Ser Ala Gly Thr Gln Glu Asp Ala Ala Ser Leu 
                325                 330                 335     


Arg Val Phe Thr Glu Ala Met Thr Arg Tyr Ser Ala Pro Pro Gly Asp 
            340                 345                 350         


Pro Pro Gln Pro Glu Tyr Asp Leu Glu Leu Ile Thr Ser Cys Ser Ser 
        355                 360                 365             


Asn Val Ser Val Ala His Asp Ala Ser Gly Lys Arg Val Tyr Tyr Leu 
    370                 375                 380                 


Thr Arg Asp Pro Thr Thr Pro Leu Ala Arg Ala Ala Trp Glu Thr Ala 
385                 390                 395                 400 


Arg His Thr Pro Val Asn Ser Trp Leu Gly Asn Ile Ile Met Tyr Ala 
                405                 410                 415     


Pro Thr Leu Trp Ala Arg Met Ile Leu Met Thr His Phe Phe Ser Ile 
            420                 425                 430         


Leu Leu Ala Gln Glu Gln Leu Glu Lys Ala Leu Asp Cys Gln Ile Tyr 
        435                 440                 445             


Gly Ala Cys Tyr Ser Ile Glu Pro Leu Asp Leu Pro Gln Ile Ile Glu 
    450                 455                 460                 


Arg Leu His Gly Leu Ser Ala Phe Ser Leu His Ser Tyr Ser Pro Gly 
465                 470                 475                 480 


Glu Ile Asn Arg Val Ala Ser Cys Leu Arg Lys Leu Gly Val Pro Pro 
                485                 490                 495     


Leu Arg Val Trp Arg His Arg Ala Arg Ser Val Arg Ala Arg Leu Leu 
            500                 505                 510         


Ser Gln Gly Gly Arg Ala Ala Thr Cys Gly Lys Tyr Leu Phe Asn Trp 
        515                 520                 525             


Ala Val Lys Thr Lys Leu Lys Leu Thr Pro Ile Pro Ala Ala Ser Gln 
    530                 535                 540                 


Leu Asp Leu Ser Gly Trp Phe Val Ala Gly Tyr Ser Gly Gly Asp Ile 
545                 550                 555                 560 


Tyr His Ser Leu Ser Arg Ala Arg Pro Arg Trp Phe Met Leu Cys Leu 
                565                 570                 575     


Leu Leu Leu Ser Val Gly Val Gly Ile Tyr Leu Leu Pro Asn Arg 
            580                 585                 590     


<210>  2
<211>  130
<212>  PRT
<213>  artificial sequence

<220>
<223>  216-345 NS5B non mute au jour 0

<400>  2

Gly Val Pro Tyr Asp Thr Arg Cys Phe Asp Ser Thr Val Thr Glu Asn 
1               5                   10                  15      


Asp Ile Arg Val Glu Glu Ser Ile Tyr Gln Cys Cys Asp Leu Ala Pro 
            20                  25                  30          


Glu Ala Arg Gln Ala Ile Arg Ser Leu Thr Glu Arg Leu Tyr Val Gly 
        35                  40                  45              


Gly Pro Leu Thr Asn Ser Lys Gly Gln Asn Cys Gly Tyr Arg Arg Cys 
    50                  55                  60                  


Arg Ala Ser Gly Val Leu Thr Thr Ser Cys Gly Asn Thr Leu Thr Cys 
65                  70                  75                  80  


Tyr Leu Lys Ala Thr Ala Ala Cys Arg Ala Ala Lys Leu Gln Asp Cys 
                85                  90                  95      


Thr Leu Leu Val Asn Gly Asp Asp Leu Val Val Ile Cys Glu Ser Ala 
            100                 105                 110         


Gly Thr Gln Glu Asp Ala Ala Asn Leu Arg Val Phe Thr Glu Ala Met 
        115                 120                 125             


Thr Arg 
    130 


<210>  3
<211>  130
<212>  PRT
<213>  artificial sequence

<220>
<223>  216-345 NS5B mute au jour 21

<400>  3

Gly Val Pro Tyr Asp Thr Arg Cys Phe Asp Ser Thr Val Thr Glu Asn 
1               5                   10                  15      


Asp Ile Arg Val Glu Glu Ser Ile Tyr Gln Cys Cys Asp Leu Ala Pro 
            20                  25                  30          


Glu Ala Arg Gln Ala Ile Arg Ser Leu Thr Glu Arg Leu Tyr Ala Gly 
        35                  40                  45              


Gly Ser Leu Thr Asn Ser Lys Gly Gln Asn Cys Gly Tyr Arg Arg Cys 
    50                  55                  60                  


Arg Ala Ser Gly Val Leu Thr Thr Ser Cys Gly Asn Thr Leu Thr Cys 
65                  70                  75                  80  


Tyr Leu Lys Ala Thr Ala Ala Cys Arg Ala Ala Lys Leu Gln Asp Cys 
                85                  90                  95      


Thr Leu Leu Val Asp Gly Asp Asp Leu Val Val Ile Cys Glu Ser Ala 
            100                 105                 110         


Gly Thr Gln Glu Asp Ala Ala Asn Leu Arg Val Phe Thr Glu Ala Met 
        115                 120                 125             


Thr Arg 
    130 


<210>  4
<211>  390
<212>  DNA
<213>  artificial sequence

<220>
<223>  216-345 NS5B non mute au jour 0

<400>  4
ggggtcccgt atgatacccg ctgctttgac tcaacggtca ctgagaatga catccgtgtt     60

gaggagtcaa tttaccaatg ttgtgactta gcccccgaag ccagacaggc tataaggtcg    120

ctcacggagc ggctttatgt cgggggcccc ctgactaatt caaaagggca gaactgcggt    180

tatcgccggt gccgcgcaag cggcgtgctg acgaccagct gcggtaatac cctcacatgt    240

tacttgaagg ccactgcggc ctgtcgagct gcaaagctcc aggactgcac attgctcgtg    300

aacggcgacg accttgtcgt tatctgcgaa agcgcgggaa cccaagagga cgcggcgaac    360

ctacgagtct tcacggaggc tatgaccagg                                     390


<210>  5
<211>  390
<212>  DNA
<213>  artificial sequence

<220>
<223>  216-345 NS5B mute au jour 21

<400>  5
ggggtcccgt atgatacccg ctgctttgac tcaacggtca ctgagaacga catccgtgtt     60

gaggagtcaa tttaccaatg ttgtgactta gcccccgaag ccagacaggc tataaggtcg    120

ctcacagagc ggctttatgc cgggggctcc ctgactaatt caaaagggca gaactgcggt    180

tatcgccggt gccgcgcgag cggcgtgctg acgactagct gcggtaatac cctcacatgt    240

tacttgaagg ccactgcggc ctgtcgagct gcgaagctcc aggactgcac attgctcgtg    300

gacggcgacg accttgttgt tatctgcgaa agcgcgggaa ctcaagagga cgcggcgaac    360

ctacgagtct tcacggaggc tatgaccagg                                     390


<210>  6
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  2CH

<400>  6
aactactgtc ttcacgcaga a                                               21


<210>  7
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  1TS

<400>  7
gcgacccaac actactcggc t                                               21


<210>  8
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TM416

<400>  8
aacccgctca atgcctgga                                                  19


