                         SEQUENCE LISTING

<110>  Emergent Product Development United Kingdom Limited
       Telfer, Jonathan
       Caproni, Lisa
 
<120>  VACCINES AGAINST CLOSTRIDIUM DIFFICILE AND METHODS OF USE

<130>  EMER-001/01WO

<150>  US 61/086,673
<151>  2008-08-06

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  2679
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  CDS
<222>  (1)..(2679)

<400>  1
aca tat tac tac gac gaa gat tcg aag ttg gtc aag ggc ctg ata aac         48
Thr Tyr Tyr Tyr Asp Glu Asp Ser Lys Leu Val Lys Gly Leu Ile Asn           
1               5                   10                  15                

ata aac aac tcg tta ttt tat ttc gat cct att gaa ttt aac ctg gtg         96
Ile Asn Asn Ser Leu Phe Tyr Phe Asp Pro Ile Glu Phe Asn Leu Val           
            20                  25                  30                    

acg ggg tgg cag acc ata aac ggg aag aag tac tac ttt gac atc aat        144
Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asp Ile Asn           
        35                  40                  45                        

acc ggc gca gca ttg att tca tat aag ata att aac ggc aag cat ttc        192
Thr Gly Ala Ala Leu Ile Ser Tyr Lys Ile Ile Asn Gly Lys His Phe           
    50                  55                  60                            

tac ttt aac aac gat gga gtc atg caa ctg gga gtc ttt aag ggt ccc        240
Tyr Phe Asn Asn Asp Gly Val Met Gln Leu Gly Val Phe Lys Gly Pro           
65                  70                  75                  80            

gac ggc ttc gaa tac ttt gcc cca gcg aac acc caa aac aac aat att        288
Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Gln Asn Asn Asn Ile           
                85                  90                  95                

gag ggg cag gcg att gtc tat caa tca aag ttt ttg acg ctg aac ggt        336
Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn Gly           
            100                 105                 110                   

aag aaa tac tat ttt gat aac gat tcg aaa gca gtc acg ggg tgg cgg        384
Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Arg           
        115                 120                 125                       

att att aac aac gaa aaa tat tat ttt aat cca aat aat gct atc gca        432
Ile Ile Asn Asn Glu Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala           
    130                 135                 140                           

gca gtc ggg ctt caa gtg atc gat aat aat aag tac tac ttc aat cca        480
Ala Val Gly Leu Gln Val Ile Asp Asn Asn Lys Tyr Tyr Phe Asn Pro           
145                 150                 155                 160           

gat acg gct att att tca aaa ggg tgg cag act gtc aac ggc tcc agg        528
Asp Thr Ala Ile Ile Ser Lys Gly Trp Gln Thr Val Asn Gly Ser Arg           
                165                 170                 175               

tat tat ttc gac act gat act gct atc gct ttc aac ggg tat aag aca        576
Tyr Tyr Phe Asp Thr Asp Thr Ala Ile Ala Phe Asn Gly Tyr Lys Thr           
            180                 185                 190                   

atc gat ggt aag cat ttc tac ttt gat agc gac tgc gtg gtt aaa att        624
Ile Asp Gly Lys His Phe Tyr Phe Asp Ser Asp Cys Val Val Lys Ile           
        195                 200                 205                       

ggt gta ttc agt acc tct aat gga ttt gag tac ttc gct cct gca aac        672
Gly Val Phe Ser Thr Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn           
    210                 215                 220                           

act tac aat aac aat att gaa ggt cag gcc atc gta tac caa agc aag        720
Thr Tyr Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys           
225                 230                 235                 240           

ttc ctc acc tta aat ggc aaa aag tac tat ttc gac aac aat agc aaa        768
Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asn Ser Lys           
                245                 250                 255               

gcg gtc acc ggt tgg cag acc att gat agt aaa aaa tat tat ttt aat        816
Ala Val Thr Gly Trp Gln Thr Ile Asp Ser Lys Lys Tyr Tyr Phe Asn           
            260                 265                 270                   

acc aac act gcg gaa gct gct acc gga tgg cag aca atc gac ggc aag        864
Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys           
        275                 280                 285                       

aag tat tat ttc aac acc aat aca gca gaa gcg gcc aca ggg tgg caa        912
Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln           
    290                 295                 300                           

acg atc gac ggg aag aag tac tac ttt aat act aac acg gcc att gct        960
Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Ile Ala           
305                 310                 315                 320           

agc acc ggt tat acc att att aat ggg aaa cac ttt tac ttc aac act       1008
Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe Tyr Phe Asn Thr           
                325                 330                 335               

gac ggc att atg cag atc ggt gta ttc aaa ggg cct aac ggc ttc gaa       1056
Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu           
            340                 345                 350                   

tat ttc gca ccg gcc aat aca gac gcg aac aat ata gaa gga cag gcg       1104
Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala           
        355                 360                 365                       

att ctg tat cag aat gaa ttc ctg acc ctg aat ggt aag aaa tat tac       1152
Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr           
    370                 375                 380                           

ttc ggc agc gat tct aag gcc gtc acc ggg tgg cgg ata atc aat aat       1200
Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg Ile Ile Asn Asn           
385                 390                 395                 400           

aaa aag tac tat ttc aac ccg aat aac gcg att gca gct att cac ctg       1248
Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala Ala Ile His Leu           
                405                 410                 415               

tgc acg atc aac aat gat aag tat tat ttt agc tat gat ggg atc ctt       1296
Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr Asp Gly Ile Leu           
            420                 425                 430                   

caa aat gga tat att aca ata gaa aga aat aac ttc tat ttc gat gcg       1344
Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe Tyr Phe Asp Ala           
        435                 440                 445                       

aat aat gag tct aaa atg gtg act ggc gtt ttc aaa ggc cca aat ggg       1392
Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys Gly Pro Asn Gly           
    450                 455                 460                           

ttc gaa tac ttc gct ccg gcg aac aca cac aac aac aat att gaa ggg       1440
Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn Asn Ile Glu Gly           
465                 470                 475                 480           

cag gca ata gtg tat cag aat aaa ttc ttg acg ctg aat ggt aaa aag       1488
Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys           
                485                 490                 495               

tac tac ttt gat aat gat tcg aaa gcg gta aca ggc tgg cag acc ata       1536
Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Gln Thr Ile           
            500                 505                 510                   

gac ggc aag aaa tat tac ttt aat ctg aat act gcc gaa gct gcg acg       1584
Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala Thr           
        515                 520                 525                       

ggc tgg caa acc ata gac gga aag aaa tat tat ttt aat ctg aac acc       1632
Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr           
    530                 535                 540                           

gca gag gcc gcc acc gga tgg cag acc atc gac ggg aag aaa tac tat       1680
Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr           
545                 550                 555                 560           

ttc aac act aat acc ttc ata gcg agt acg ggg tat acc tcg atc aat       1728
Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr Thr Ser Ile Asn           
                565                 570                 575               

ggc aag cat ttc tac ttt aac acc gac ggg att atg cag atc ggt gtt       1776
Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val           
            580                 585                 590                   

ttc aag ggg ccg aac ggc ttc gaa tac ttc gct ccc gca aac aca cac       1824
Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His           
        595                 600                 605                       

aac aac aac atc gag gga cag gct ata ctg tat caa aat aaa ttt ctt       1872
Asn Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Lys Phe Leu           
    610                 615                 620                           

acg tta aat ggc aag aag tat tat ttt ggg tcg gac agc aaa gca gtg       1920
Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val           
625                 630                 635                 640           

acc ggt ttg cgt acc ata gat ggt aag aaa tat tat ttt aat act aac       1968
Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn           
                645                 650                 655               

acg gca gta gcc gtt acc gga tgg cag act att aat ggg aag aaa tac       2016
Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr           
            660                 665                 670                   

tat ttt aac act aac acg agc att gcc tcg act ggc tac acg atc att       2064
Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly Tyr Thr Ile Ile           
        675                 680                 685                       

agc ggg aaa cac ttc tac ttc aac acg gat ggt att atg cag ata ggt       2112
Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly           
    690                 695                 700                           

gtc ttt aaa ggt cct gac ggt ttt gag tac ttc gca ccc gcc aac acc       2160
Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr           
705                 710                 715                 720           

gac gct aat aac ata gag ggg caa gct atc agg tat cag aat cgc ttc       2208
Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe           
                725                 730                 735               

ctt tac ctg cat gat aac atc tat tac ttc ggg aac aac agt aag gct       2256
Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala           
            740                 745                 750                   

gct acc ggg tgg gtg aca att gac ggt aat cgc tat tat ttc gag cct       2304
Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr Tyr Phe Glu Pro           
        755                 760                 765                       

aac aca gca atg gga gcc aat ggc tat aag act atc gat aac aaa aat       2352
Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile Asp Asn Lys Asn           
    770                 775                 780                           

ttt tac ttt cgg aac ggt ttg cct caa atc ggg gtt ttt aaa gga tct       2400
Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val Phe Lys Gly Ser           
785                 790                 795                 800           

aac ggc ttc gag tac ttt gcc ccg gcg aac acg gat gcc aac aat att       2448
Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile           
                805                 810                 815               

gag ggc cag gcg ata agg tac cag aac cgc ttt ctg cat ctc ttg ggt       2496
Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu His Leu Leu Gly           
            820                 825                 830                   

aaa atc tat tac ttc ggc aac aac tca aag gcg gta aca gga tgg caa       2544
Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Val Thr Gly Trp Gln           
        835                 840                 845                       

act ata aac ggg aag gtt tac tat ttt atg cct gat acg gcc atg gct       2592
Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met Pro Asp Thr Ala Met Ala           
    850                 855                 860                           

gcg gcg gga ggc ctg ttc gaa att gac ggt gtt ata tac ttt ttc ggt       2640
Ala Ala Gly Gly Leu Phe Glu Ile Asp Gly Val Ile Tyr Phe Phe Gly           
865                 870                 875                 880           

gtg gac ggt gtt aag gcc cca ggc att tac ccc ggg taa                   2679
Val Asp Gly Val Lys Ala Pro Gly Ile Tyr Pro Gly                           
                885                 890                                   


<210>  2
<211>  892
<212>  PRT
<213>  Clostridium difficile

<400>  2

Thr Tyr Tyr Tyr Asp Glu Asp Ser Lys Leu Val Lys Gly Leu Ile Asn 
1               5                   10                  15      


Ile Asn Asn Ser Leu Phe Tyr Phe Asp Pro Ile Glu Phe Asn Leu Val 
            20                  25                  30          


Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asp Ile Asn 
        35                  40                  45              


Thr Gly Ala Ala Leu Ile Ser Tyr Lys Ile Ile Asn Gly Lys His Phe 
    50                  55                  60                  


Tyr Phe Asn Asn Asp Gly Val Met Gln Leu Gly Val Phe Lys Gly Pro 
65                  70                  75                  80  


Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Gln Asn Asn Asn Ile 
                85                  90                  95      


Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn Gly 
            100                 105                 110         


Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Arg 
        115                 120                 125             


Ile Ile Asn Asn Glu Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala 
    130                 135                 140                 


Ala Val Gly Leu Gln Val Ile Asp Asn Asn Lys Tyr Tyr Phe Asn Pro 
145                 150                 155                 160 


Asp Thr Ala Ile Ile Ser Lys Gly Trp Gln Thr Val Asn Gly Ser Arg 
                165                 170                 175     


Tyr Tyr Phe Asp Thr Asp Thr Ala Ile Ala Phe Asn Gly Tyr Lys Thr 
            180                 185                 190         


Ile Asp Gly Lys His Phe Tyr Phe Asp Ser Asp Cys Val Val Lys Ile 
        195                 200                 205             


Gly Val Phe Ser Thr Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn 
    210                 215                 220                 


Thr Tyr Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys 
225                 230                 235                 240 


Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asn Ser Lys 
                245                 250                 255     


Ala Val Thr Gly Trp Gln Thr Ile Asp Ser Lys Lys Tyr Tyr Phe Asn 
            260                 265                 270         


Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys 
        275                 280                 285             


Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln 
    290                 295                 300                 


Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Ile Ala 
305                 310                 315                 320 


Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe Tyr Phe Asn Thr 
                325                 330                 335     


Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu 
            340                 345                 350         


Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala 
        355                 360                 365             


Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr 
    370                 375                 380                 


Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg Ile Ile Asn Asn 
385                 390                 395                 400 


Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala Ala Ile His Leu 
                405                 410                 415     


Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr Asp Gly Ile Leu 
            420                 425                 430         


Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe Tyr Phe Asp Ala 
        435                 440                 445             


Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys Gly Pro Asn Gly 
    450                 455                 460                 


Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn Asn Ile Glu Gly 
465                 470                 475                 480 


Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys 
                485                 490                 495     


Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Gln Thr Ile 
            500                 505                 510         


Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala Thr 
        515                 520                 525             


Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr 
    530                 535                 540                 


Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr 
545                 550                 555                 560 


Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr Thr Ser Ile Asn 
                565                 570                 575     


Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val 
            580                 585                 590         


Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His 
        595                 600                 605             


Asn Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Lys Phe Leu 
    610                 615                 620                 


Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val 
625                 630                 635                 640 


Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn 
                645                 650                 655     


Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr 
            660                 665                 670         


Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly Tyr Thr Ile Ile 
        675                 680                 685             


Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly 
    690                 695                 700                 


Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr 
705                 710                 715                 720 


Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe 
                725                 730                 735     


Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala 
            740                 745                 750         


Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr Tyr Phe Glu Pro 
        755                 760                 765             


Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile Asp Asn Lys Asn 
    770                 775                 780                 


Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val Phe Lys Gly Ser 
785                 790                 795                 800 


Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile 
                805                 810                 815     


Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu His Leu Leu Gly 
            820                 825                 830         


Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Val Thr Gly Trp Gln 
        835                 840                 845             


Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met Pro Asp Thr Ala Met Ala 
    850                 855                 860                 


Ala Ala Gly Gly Leu Phe Glu Ile Asp Gly Val Ile Tyr Phe Phe Gly 
865                 870                 875                 880 


Val Asp Gly Val Lys Ala Pro Gly Ile Tyr Pro Gly 
                885                 890         


<210>  3
<211>  1635
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  CDS
<222>  (1)..(1635)

<400>  3
aag ttt tat atc aac aac ttc ggc atg atg gtg tct ggc ttg atc tac         48
Lys Phe Tyr Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Ile Tyr           
1               5                   10                  15                

atc aac gat agc ctc tat tat ttc aag ccg ccc gtt aat aac tta atc         96
Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn Leu Ile           
            20                  25                  30                    

aca ggc ttc gtg aca gta ggt gat gac aaa tac tat ttt aat ccg atc        144
Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro Ile           
        35                  40                  45                        

aat gga ggc gca gca agt att ggt gaa acg ata atc gac gac aag aac        192
Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys Asn           
    50                  55                  60                            

tat tat ttt aac caa tca gga gtg ctg caa act ggt gtg ttt tcc acc        240
Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser Thr           
65                  70                  75                  80            

gag gac ggc ttt aag tac ttc gcc ccc gcg aac acc ctg gac gaa aac        288
Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu Asn           
                85                  90                  95                

ctt gag ggt gaa gcc att gac ttc act ggt aaa ctt att atc gac gaa        336
Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp Glu           
            100                 105                 110                   

aac atc tac tat ttt gat gat aac tac aga ggc gca gtg gag tgg aaa        384
Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp Lys           
        115                 120                 125                       

gag ctg gac ggg gaa atg cat tac ttt tcc cca gag aca ggt aaa gct        432
Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys Ala           
    130                 135                 140                           

ttc aaa ggt ctg aat cag att ggg gat tac aaa tat tac ttc aac tct        480
Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn Ser           
145                 150                 155                 160           

gac ggt gtc atg cag aag gga ttt gtg tca atc aac gat aat aag cac        528
Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys His           
                165                 170                 175               

tac ttt gat gac tca gga gta atg aag gtg ggc tac acg gag att gac        576
Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile Asp           
            180                 185                 190                   

gga aaa cat ttc tat ttc gcc gaa aat ggt gaa atg cag att ggc gtt        624
Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly Val           
        195                 200                 205                       

ttc aat acc gag gat ggc ttc aag tat ttt gct cat cac aat gag gat        672
Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu Asp           
    210                 215                 220                           

ctg gga aac gaa gaa ggc gag gaa att tcc tac tcg ggc ata ctg aat        720
Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn           
225                 230                 235                 240           

ttt aac aat aaa ata tat tat ttc gac gac agt ttt acg gcg gtt gtt        768
Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val           
                245                 250                 255               

ggg tgg aag gat tta gaa gat ggt agt aaa tac tac ttc gat gag gac        816
Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp           
            260                 265                 270                   

acg gcc gaa gcc tat atc ggt ttg tcg ctg att aat gat gga cag tac        864
Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr           
        275                 280                 285                       

tat ttt aat gac gac ggc att atg caa gtt ggg ttc gtg acc att aac        912
Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile Asn           
    290                 295                 300                           

gac aaa gtg ttt tat ttt tca gac tca gga att atc gag agc ggg gtt        960
Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly Val           
305                 310                 315                 320           

caa aac att gat gat aat tat ttt tac ata gac gat aat ggg atc gtt       1008
Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val           
                325                 330                 335               

cag atc ggg gtg ttc gac aca tct gac ggt tac aaa tat ttt gct ccc       1056
Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro           
            340                 345                 350                   

gca aat acg gtg aac gac aac att tac ggg cag gca gtg gaa tat tcg       1104
Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser           
        355                 360                 365                       

ggt ttg gtt aga gtt ggc gag gat gtc tac tat ttt ggc gag aca tac       1152
Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr           
    370                 375                 380                           

acg att gaa acg ggg tgg att tac gat atg gag aac gaa agc gat aaa       1200
Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys           
385                 390                 395                 400           

tat tac ttt aac cca gaa aca aag aag gcc tgc aaa ggt atc aat tta       1248
Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu           
                405                 410                 415               

atc gat gat atc aaa tac tat ttc gac gaa aag ggt atc atg cgt act       1296
Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr           
            420                 425                 430                   

ggg ctg atc agc ttt gag aac aat aat tac tat ttc aat gaa aat ggg       1344
Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly           
        435                 440                 445                       

gaa atg caa ttt gga tat att aat ata gaa gat aag atg ttt tat ttc       1392
Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe           
    450                 455                 460                           

ggg gag gat ggt gtg atg cag atc ggc gtt ttc aac acc ccg gac ggg       1440
Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly           
465                 470                 475                 480           

ttt aaa tat ttc gca cat cag aat aca ctg gat gag aac ttc gag ggt       1488
Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly           
                485                 490                 495               

gag tct att aac tac acc ggg tgg ctg gac tta gac gag aaa cgc tac       1536
Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr           
            500                 505                 510                   

tat ttc aca gac gag tac att gca gct act ggt tcg gtc atc att gat       1584
Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp           
        515                 520                 525                       

ggc gag gaa tat tat ttc gac ccg gat acc gcc cag tta gtg atc tcc       1632
Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser           
    530                 535                 540                           

gag                                                                   1635
Glu                                                                       
545                                                                       


<210>  4
<211>  545
<212>  PRT
<213>  Clostridium difficile

<400>  4

Lys Phe Tyr Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Ile Tyr 
1               5                   10                  15      


Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn Leu Ile 
            20                  25                  30          


Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro Ile 
        35                  40                  45              


Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys Asn 
    50                  55                  60                  


Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser Thr 
65                  70                  75                  80  


Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu Asn 
                85                  90                  95      


Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp Glu 
            100                 105                 110         


Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp Lys 
        115                 120                 125             


Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys Ala 
    130                 135                 140                 


Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn Ser 
145                 150                 155                 160 


Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys His 
                165                 170                 175     


Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile Asp 
            180                 185                 190         


Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly Val 
        195                 200                 205             


Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu Asp 
    210                 215                 220                 


Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn 
225                 230                 235                 240 


Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val 
                245                 250                 255     


Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp 
            260                 265                 270         


Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr 
        275                 280                 285             


Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile Asn 
    290                 295                 300                 


Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly Val 
305                 310                 315                 320 


Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val 
                325                 330                 335     


Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro 
            340                 345                 350         


Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser 
        355                 360                 365             


Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr 
    370                 375                 380                 


Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys 
385                 390                 395                 400 


Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu 
                405                 410                 415     


Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr 
            420                 425                 430         


Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly 
        435                 440                 445             


Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe 
    450                 455                 460                 


Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly 
465                 470                 475                 480 


Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly 
                485                 490                 495     


Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr 
            500                 505                 510         


Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp 
        515                 520                 525             


Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser 
    530                 535                 540                 


Glu 
545 


<210>  5
<211>  305
<212>  PRT
<213>  Salmonella typhi

<400>  5

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Gly Ala Ala Ala Gly Ile Val Ala Gly Pro Phe Gly Leu 
            180                 185                 190         


Ile Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Ala 
    290                 295                 300                 


Ser 
305 


<210>  6
<211>  305
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Modified ClyA sequence

<400>  6

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu 
            180                 185                 190         


Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Ile Ser Gly Lys Lys Thr Leu Phe Glu Val Pro Asp 
    290                 295                 300                 


Val 
305 


<210>  7
<211>  1102
<212>  DNA
<213>  Salmonella typhi

<400>  7
ggaggtaata ggtaagaata ctttataaaa caggtactta attgcaattt atatatttaa       60

agaggcaaat gattatgacc ggaatatttg cagaacaaac tgtagaggta gttaaaagcg      120

cgatcgaaac cgcagatggg gcattagatc tttataacaa atacctcgac caggtcatcc      180

cctggaagac ctttgatgaa accataaaag agttaagccg ttttaaacag gagtactcgc      240

aggaagcttc tgttttagtt ggtgatatta aagttttgct tatggacagc caggacaagt      300

attttgaagc gacacaaact gtttatgaat ggtgtggtgt cgtgacgcaa ttactctcag      360

cgtatatttt actatttgat gaatataatg agaaaaaagc atcagcccag aaagacattc      420

tcattaggat attagatgat ggtgtcaaga aactgaatga agcgcaaaaa tctctcctga      480

caagttcaca aagtttcaac aacgcttccg gaaaactgct ggcattagat agccagttaa      540

ctaatgattt ttcggaaaaa agtagttatt tccagtcaca ggtggataga attcgtaagg      600

aagcttatgc cggtgctgca gccggcatag tcgccggtcc gtttggatta attatttcct      660

attctattgc tgcgggcgtg attgaaggga aattgattcc agaattgaat aacaggctaa      720

aaacagtgca aaatttcttt actagcttat cagctacagt gaaacaagcg aataaagata      780

tcgatgcggc aaaattgaaa ttagccactg aaatagcagc aattggggag ataaaaacgg      840

aaaccgaaac aaccagattc tacgttgatt atgatgattt aatgctttct ttattaaaag      900

gagctgcaaa gaaaatgatt aacacctgta atgaatacca acaaagacac ggtaagaaga      960

cgcttttcga ggttcctgac gtctgataca ttttcattcg atctgtgtac ttttaacgcc     1020

cgatagcgta aagaaaatga gagacggaga aaaagcgata ttcaacagcc cgataaacaa     1080

gagtcgttac cgggctgacg ag                                              1102


<210>  8
<211>  1102
<212>  DNA
<213>  Salmonella paratyphi

<400>  8
ggaggcaata ggtaggaata agttataaaa caatagctta attgcaattt atatatttaa       60

agaggcaaat gattatgact ggaatatttg cagaacaaac tgtagaggta gttaaaagcg      120

cgatcgaaac cgcagatggg gcattagatt tttataacaa atacctcgac caggttatcc      180

cctggaagac ctttgatgaa accataaaag agttaagccg ttttaaacag gagtactcgc      240

aggaagcttc tgttttagtt ggtgatatta aagttttgct tatggacagc caggataagt      300

attttgaagc gacacaaact gtttatgaat ggtgtggtgt cgtgacgcaa ttactctcag      360

cgtatatttt actatttgat gaatataatg agaaaaaagc atcagcgcag aaagacattc      420

tcatcaggat attagatgat ggcgtcaata aactgaatga agcgcaaaaa tctctcctgg      480

gaagttcaca aagtttcaac aacgcttcag gaaaactgct ggcattagat agccagttaa      540

ctaatgattt ctcggaaaaa agtagttatt tccagtcaca ggtggataga attcgtaagg      600

aagcttatgc cggtgctgca gcaggcatag tcgccggtcc gtttggatta attatttcct      660

attctattgc tgcgggcgtg attgaaggga aattgattcc agaattgaat gacaggctaa      720

aagcagtgca aaatttcttt actagcttat cagtcacagt gaaacaagcg aataaagata      780

tcgatgcggc aaaattgaaa ttagccactg aaatagcagc aattggggag ataaaaacgg      840

aaaccgaaac aaccagattc tacgttgatt atgatgattt aatgctttct ttactaaaag      900

gagctgcaaa gaaaatgatt aacacctgta atgaatacca acaaaggcac ggtaagaaga      960

cgcttctcga ggttcctgac atctgataca ttttcattcg ctctgtttac ttttaacgcc     1020

cgatagcgtg aagaaaatga gagacggaga aaaagcgata ttcaacagcc cgataaacaa     1080

gagtcgttac cgggctggcg ag                                              1102


<210>  9
<211>  904
<212>  DNA
<213>  Shigella flexneri

<400>  9
atgactgaaa tcgttgcaga taaaacggta gaagtagtta aaaacgcaat cgaaaccgca       60

gatggagcat tagatcttta taataaatat ctcgatcagg tcatcccctg gcagaccttt      120

gatgaaacca taaaagagtt aagtcgcttt aaacaggagt attcacaggc agcctccgtt      180

ttagtcggcg atattaaaac cttacttatg gatagccagg ataagtattt tgaagcaacc      240

caaacagtgt atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta tattttgcta      300

tttgatgagt acaatgagaa gaaagcatcc gcccctcatt aaggtactgg atgacggcat      360

cacgaagctg aatgaagcgc aaaattccct gctggtaagc tcacaaagtt tcaacaacgc      420

ttccgggaaa ctgctggcgt tagatagcca gttaaccaat gatttttcag aaaaaagcag      480

ctatttccag tcacaggtag ataaaatcag gaaggaagcg tatgccggtg ccgcagccgg      540

tgtcgtcgcc ggtccatttg gtttaatcat ttcctattct attgctgcgg gcgtagttga      600

agggaaactg attccagaat tgaagaacaa gttaaaatct gtgcagagtt tctttaccac      660

cctgtctaac acggttaaac aagcgaataa agatatcgat gccgccaaat tgaaattaac      720

caccgaaata gccgccatcg gggagataaa aacggaaact gaaaccacca gattctatgt      780

tgattatgat gatttaatgc tttctttgct aaaagcagcg gccaaaaaaa tgattaacac      840

ctgtaatgag tatcagaaaa gacacggtaa aaagacactc tttgaggtac ctgaagtctg      900

ataa                                                                   904


<210>  10
<211>  1080
<212>  DNA
<213>  Escherichia coli

<400>  10
agaaataaag acattgacgc atcccgcccg gctaactatg aattagatga agtaaaattt       60

attaatagtt gtaaaacagg agtttcatta caatttatat atttaaagag gcgaatgatt      120

atgactgaaa tcgttgcaga taaaacggta gaagtagtta aaaacgcaat cgaaaccgca      180

gatggagcat tagatcttta taataaatat ctcgatcagg tcatcccctg gcagaccttt      240

gatgaaacca taaaagagtt aagtcgcttt aaacaggagt attcacaggc agcctccgtt      300

ttagtcggcg atattaaaac cttacttatg gatagccagg ataagtattt tgaagcaacc      360

caaacagtgt atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta tattttgcta      420

tttgatgagt acaatgagaa gaaagcatcc gcccagaaag acattctcat taaggtactg      480

gatgacggca tcacgaagct gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt      540

ttcaacaacg cttccgggaa actgctggcg ttagatagcc agttaaccaa tgatttttca      600

gaaaaaagca gctatttcca gtcacaggta gataaaatca ggaaggaagc atatgccggt      660

gccgcagccg gtgtcgtcgc cggtccattt ggattaatca tttcctattc tattgctgcg      720

ggcgtagttg aaggaaaact gattccagaa ttgaagaaca agttaaaatc tgtgcagaat      780

ttctttacca ccctgtctaa cacggttaaa caagcgaata aagatatcga tgccgccaaa      840

ttgaaattaa ccaccgaaat agccgccatc ggtgagataa aaacggaaac tgaaacaacc      900

agattctacg ttgattatga tgatttaatg ctttctttgc taaaagaagc ggccaaaaaa      960

atgattaaca cctgtaatga gtatcagaaa agacacggta aaaagacact ctttgaggta     1020

cctgaagtct gataagcgat tattctctcc atgtactcaa ggtataaggt ttatcacatt     1080


<210>  11
<211>  50
<212>  PRT
<213>  Salmonella typhi

<400>  11

Met Ser Phe Ser Arg Arg Gln Phe Leu Gln Ala Ser Gly Ile Ala Leu 
1               5                   10                  15      


Cys Ala Gly Ala Ile Pro Leu Arg Ala Asn Ala Ala Gly Gln Gln Gln 
            20                  25                  30          


Pro Leu Pro Val Pro Pro Leu Leu Glu Ser Arg Arg Gly Gln Pro Leu 
        35                  40                  45              


Phe Met 
    50  


<210>  12
<211>  3594
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion A Codon-optimized sequence


<220>
<221>  CDS
<222>  (1)..(3594)

<400>  12
atg act tcg atc ttc gcc gaa cag acg gtt gag gtg gta aaa tca gcc         48
Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala           
1               5                   10                  15                

ata gaa acc gcg gat ggg gcg ctc gac ctt tac aat aag tac ctt gat         96
Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp           
            20                  25                  30                    

cag gtg atc ccg tgg aaa acg ttc gac gag act atc aaa gaa tta tca        144
Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser           
        35                  40                  45                        

cga ttt aag cag gaa tat tca cag gaa gca tcc gta ctt gtt ggt gat        192
Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp           
    50                  55                  60                            

att aaa gtc tta ctc atg gat tct cag gat aag tac ttc gag gca acc        240
Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr           
65                  70                  75                  80            

cag acg gtg tac gag tgg tgt ggc gtt gta aca cag ctt ctg tcg gct        288
Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala           
                85                  90                  95                

tac att ctt ctg ttc gat gaa tat aac gag aaa aaa gcc tcc gcc cag        336
Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln           
            100                 105                 110                   

aaa gac att ctg ata cgc att ctt gac gat ggt gtg aag aag ctg aac        384
Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn           
        115                 120                 125                       

gaa gca cag aaa tcg tta tta act tcc tct cag tcc ttt aat aac gcg        432
Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala           
    130                 135                 140                           

tca ggc aag tta ctg gct ctt gat tcc cag ttg act aat gac ttc agt        480
Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser           
145                 150                 155                 160           

gaa aaa tcg tcg tat ttc cag tca caa gtt gac cgt atc cgt aaa gag        528
Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu           
                165                 170                 175               

gct tac gct gtc gct gct gcg ggc tcg gtc agt ggc cca ttc ggt ctt        576
Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu           
            180                 185                 190                   

tct atc agc tat agc att gca gcc gga gtc ata gaa ggc aaa ctg atc        624
Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile           
        195                 200                 205                       

ccg gag ttg aac aat cgc ctg aaa acc gtg caa aat ttt ttt acg agt        672
Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser           
    210                 215                 220                           

ttg agc gcc act gtc aaa cag gcg aac aag gat ata gat gct gca aaa        720
Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys           
225                 230                 235                 240           

ctc aaa tta gcg acc gaa att gcc gcg ata ggt gaa att aag acc gaa        768
Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu           
                245                 250                 255               

acg gag aca acc cgg ttc tac gtc gac tac gac gac ttg atg tta tca        816
Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser           
            260                 265                 270                   

ttg ctg aaa ggc gcc gct aaa aag atg atc aac acc tgt aac gaa tat        864
Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr           
        275                 280                 285                       

cag cag cgg cac gga aaa aaa acc ctt ttt gag gtc cct gat gtc ggg        912
Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Gly           
    290                 295                 300                           

ccc aca tat tac tac gac gaa gat tcg aag ttg gtc aag ggc ctg ata        960
Pro Thr Tyr Tyr Tyr Asp Glu Asp Ser Lys Leu Val Lys Gly Leu Ile           
305                 310                 315                 320           

aac ata aac aac tcg tta ttt tat ttc gat cct att gaa ttt aac ctg       1008
Asn Ile Asn Asn Ser Leu Phe Tyr Phe Asp Pro Ile Glu Phe Asn Leu           
                325                 330                 335               

gtg acg ggg tgg cag acc ata aac ggg aag aag tac tac ttt gac atc       1056
Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asp Ile           
            340                 345                 350                   

aat acc ggc gca gca ttg att tca tat aag ata att aac ggc aag cat       1104
Asn Thr Gly Ala Ala Leu Ile Ser Tyr Lys Ile Ile Asn Gly Lys His           
        355                 360                 365                       

ttc tac ttt aac aac gat gga gtc atg caa ctg gga gtc ttt aag ggt       1152
Phe Tyr Phe Asn Asn Asp Gly Val Met Gln Leu Gly Val Phe Lys Gly           
    370                 375                 380                           

ccc gac ggc ttc gaa tac ttt gcc cca gcg aac acc caa aac aac aat       1200
Pro Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Gln Asn Asn Asn           
385                 390                 395                 400           

att gag ggg cag gcg att gtc tat caa tca aag ttt ttg acg ctg aac       1248
Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn           
                405                 410                 415               

ggt aag aaa tac tat ttt gat aac gat tcg aaa gca gtc acg ggg tgg       1296
Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp           
            420                 425                 430                   

cgg att att aac aac gaa aaa tat tat ttt aat cca aat aat gct atc       1344
Arg Ile Ile Asn Asn Glu Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile           
        435                 440                 445                       

gca gca gtc ggg ctt caa gtg atc gat aat aat aag tac tac ttc aat       1392
Ala Ala Val Gly Leu Gln Val Ile Asp Asn Asn Lys Tyr Tyr Phe Asn           
    450                 455                 460                           

cca gat acg gct att att tca aaa ggg tgg cag act gtc aac ggc tcc       1440
Pro Asp Thr Ala Ile Ile Ser Lys Gly Trp Gln Thr Val Asn Gly Ser           
465                 470                 475                 480           

agg tat tat ttc gac act gat act gct atc gct ttc aac ggg tat aag       1488
Arg Tyr Tyr Phe Asp Thr Asp Thr Ala Ile Ala Phe Asn Gly Tyr Lys           
                485                 490                 495               

aca atc gat ggt aag cat ttc tac ttt gat agc gac tgc gtg gtt aaa       1536
Thr Ile Asp Gly Lys His Phe Tyr Phe Asp Ser Asp Cys Val Val Lys           
            500                 505                 510                   

att ggt gta ttc agt acc tct aat gga ttt gag tac ttc gct cct gca       1584
Ile Gly Val Phe Ser Thr Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala           
        515                 520                 525                       

aac act tac aat aac aat att gaa ggt cag gcc atc gta tac caa agc       1632
Asn Thr Tyr Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser           
    530                 535                 540                           

aag ttc ctc acc tta aat ggc aaa aag tac tat ttc gac aac aat agc       1680
Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asn Ser           
545                 550                 555                 560           

aaa gcg gtc acc ggt tgg cag acc att gat agt aaa aaa tat tat ttt       1728
Lys Ala Val Thr Gly Trp Gln Thr Ile Asp Ser Lys Lys Tyr Tyr Phe           
                565                 570                 575               

aat acc aac act gcg gaa gct gct acc gga tgg cag aca atc gac ggc       1776
Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly           
            580                 585                 590                   

aag aag tat tat ttc aac acc aat aca gca gaa gcg gcc aca ggg tgg       1824
Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp           
        595                 600                 605                       

caa acg atc gac ggg aag aag tac tac ttt aat act aac acg gcc att       1872
Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Ile           
    610                 615                 620                           

gct agc acc ggt tat acc att att aat ggg aaa cac ttt tac ttc aac       1920
Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe Tyr Phe Asn           
625                 630                 635                 640           

act gac ggc att atg cag atc ggt gta ttc aaa ggg cct aac ggc ttc       1968
Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe           
                645                 650                 655               

gaa tat ttc gca ccg gcc aat aca gac gcg aac aat ata gaa gga cag       2016
Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln           
            660                 665                 670                   

gcg att ctg tat cag aat gaa ttc ctg acc ctg aat ggt aag aaa tat       2064
Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly Lys Lys Tyr           
        675                 680                 685                       

tac ttc ggc agc gat tct aag gcc gtc acc ggg tgg cgg ata atc aat       2112
Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg Ile Ile Asn           
    690                 695                 700                           

aat aaa aag tac tat ttc aac ccg aat aac gcg att gca gct att cac       2160
Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala Ala Ile His           
705                 710                 715                 720           

ctg tgc acg atc aac aat gat aag tat tat ttt agc tat gat ggg atc       2208
Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr Asp Gly Ile           
                725                 730                 735               

ctt caa aat gga tat att aca ata gaa aga aat aac ttc tat ttc gat       2256
Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe Tyr Phe Asp           
            740                 745                 750                   

gcg aat aat gag tct aaa atg gtg act ggc gtt ttc aaa ggc cca aat       2304
Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys Gly Pro Asn           
        755                 760                 765                       

ggg ttc gaa tac ttc gct ccg gcg aac aca cac aac aac aat att gaa       2352
Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn Asn Ile Glu           
    770                 775                 780                           

ggg cag gca ata gtg tat cag aat aaa ttc ttg acg ctg aat ggt aaa       2400
Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys           
785                 790                 795                 800           

aag tac tac ttt gat aat gat tcg aaa gcg gta aca ggc tgg cag acc       2448
Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Gln Thr           
                805                 810                 815               

ata gac ggc aag aaa tat tac ttt aat ctg aat act gcc gaa gct gcg       2496
Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala           
            820                 825                 830                   

acg ggc tgg caa acc ata gac gga aag aaa tat tat ttt aat ctg aac       2544
Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn           
        835                 840                 845                       

acc gca gag gcc gcc acc gga tgg cag acc atc gac ggg aag aaa tac       2592
Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr           
    850                 855                 860                           

tat ttc aac act aat acc ttc ata gcg agt acg ggg tat acc tcg atc       2640
Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr Thr Ser Ile           
865                 870                 875                 880           

aat ggc aag cat ttc tac ttt aac acc gac ggg att atg cag atc ggt       2688
Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly           
                885                 890                 895               

gtt ttc aag ggg ccg aac ggc ttc gaa tac ttc gct ccc gca aac aca       2736
Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr           
            900                 905                 910                   

cac aac aac aac atc gag gga cag gct ata ctg tat caa aat aaa ttt       2784
His Asn Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Lys Phe           
        915                 920                 925                       

ctt acg tta aat ggc aag aag tat tat ttt ggg tcg gac agc aaa gca       2832
Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala           
    930                 935                 940                           

gtg acc ggt ttg cgt acc ata gat ggt aag aaa tat tat ttt aat act       2880
Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr           
945                 950                 955                 960           

aac acg gca gta gcc gtt acc gga tgg cag act att aat ggg aag aaa       2928
Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys           
                965                 970                 975               

tac tat ttt aac act aac acg agc att gcc tcg act ggc tac acg atc       2976
Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly Tyr Thr Ile           
            980                 985                 990                   

att agc ggg aaa cac ttc tac ttc  aac acg gat ggt att  atg cag ata     3024
Ile Ser Gly Lys His Phe Tyr Phe  Asn Thr Asp Gly Ile  Met Gln Ile         
        995                 1000                 1005                     

ggt gtc  ttt aaa ggt cct gac  ggt ttt gag tac ttc  gca ccc gcc        3069
Gly Val  Phe Lys Gly Pro Asp  Gly Phe Glu Tyr Phe  Ala Pro Ala            
    1010                 1015                 1020                        

aac acc  gac gct aat aac ata  gag ggg caa gct atc  agg tat cag        3114
Asn Thr  Asp Ala Asn Asn Ile  Glu Gly Gln Ala Ile  Arg Tyr Gln            
    1025                 1030                 1035                        

aat cgc  ttc ctt tac ctg cat  gat aac atc tat tac  ttc ggg aac        3159
Asn Arg  Phe Leu Tyr Leu His  Asp Asn Ile Tyr Tyr  Phe Gly Asn            
    1040                 1045                 1050                        

aac agt  aag gct gct acc ggg  tgg gtg aca att gac  ggt aat cgc        3204
Asn Ser  Lys Ala Ala Thr Gly  Trp Val Thr Ile Asp  Gly Asn Arg            
    1055                 1060                 1065                        

tat tat  ttc gag cct aac aca  gca atg gga gcc aat  ggc tat aag        3249
Tyr Tyr  Phe Glu Pro Asn Thr  Ala Met Gly Ala Asn  Gly Tyr Lys            
    1070                 1075                 1080                        

act atc  gat aac aaa aat ttt  tac ttt cgg aac ggt  ttg cct caa        3294
Thr Ile  Asp Asn Lys Asn Phe  Tyr Phe Arg Asn Gly  Leu Pro Gln            
    1085                 1090                 1095                        

atc ggg  gtt ttt aaa gga tct  aac ggc ttc gag tac  ttt gcc ccg        3339
Ile Gly  Val Phe Lys Gly Ser  Asn Gly Phe Glu Tyr  Phe Ala Pro            
    1100                 1105                 1110                        

gcg aac  acg gat gcc aac aat  att gag ggc cag gcg  ata agg tac        3384
Ala Asn  Thr Asp Ala Asn Asn  Ile Glu Gly Gln Ala  Ile Arg Tyr            
    1115                 1120                 1125                        

cag aac  cgc ttt ctg cat ctc  ttg ggt aaa atc tat  tac ttc ggc        3429
Gln Asn  Arg Phe Leu His Leu  Leu Gly Lys Ile Tyr  Tyr Phe Gly            
    1130                 1135                 1140                        

aac aac  tca aag gcg gta aca  gga tgg caa act ata  aac ggg aag        3474
Asn Asn  Ser Lys Ala Val Thr  Gly Trp Gln Thr Ile  Asn Gly Lys            
    1145                 1150                 1155                        

gtt tac  tat ttt atg cct gat  acg gcc atg gct gcg  gcg gga ggc        3519
Val Tyr  Tyr Phe Met Pro Asp  Thr Ala Met Ala Ala  Ala Gly Gly            
    1160                 1165                 1170                        

ctg ttc  gaa att gac ggt gtt  ata tac ttt ttc ggt  gtg gac ggt        3564
Leu Phe  Glu Ile Asp Gly Val  Ile Tyr Phe Phe Gly  Val Asp Gly            
    1175                 1180                 1185                        

gtt aag  gcc cca ggc att tac  ccc ggg taa                             3594
Val Lys  Ala Pro Gly Ile Tyr  Pro Gly                                     
    1190                 1195                                             


<210>  13
<211>  1197
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu 
            180                 185                 190         


Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Gly 
    290                 295                 300                 


Pro Thr Tyr Tyr Tyr Asp Glu Asp Ser Lys Leu Val Lys Gly Leu Ile 
305                 310                 315                 320 


Asn Ile Asn Asn Ser Leu Phe Tyr Phe Asp Pro Ile Glu Phe Asn Leu 
                325                 330                 335     


Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asp Ile 
            340                 345                 350         


Asn Thr Gly Ala Ala Leu Ile Ser Tyr Lys Ile Ile Asn Gly Lys His 
        355                 360                 365             


Phe Tyr Phe Asn Asn Asp Gly Val Met Gln Leu Gly Val Phe Lys Gly 
    370                 375                 380                 


Pro Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Gln Asn Asn Asn 
385                 390                 395                 400 


Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn 
                405                 410                 415     


Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp 
            420                 425                 430         


Arg Ile Ile Asn Asn Glu Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile 
        435                 440                 445             


Ala Ala Val Gly Leu Gln Val Ile Asp Asn Asn Lys Tyr Tyr Phe Asn 
    450                 455                 460                 


Pro Asp Thr Ala Ile Ile Ser Lys Gly Trp Gln Thr Val Asn Gly Ser 
465                 470                 475                 480 


Arg Tyr Tyr Phe Asp Thr Asp Thr Ala Ile Ala Phe Asn Gly Tyr Lys 
                485                 490                 495     


Thr Ile Asp Gly Lys His Phe Tyr Phe Asp Ser Asp Cys Val Val Lys 
            500                 505                 510         


Ile Gly Val Phe Ser Thr Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala 
        515                 520                 525             


Asn Thr Tyr Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser 
    530                 535                 540                 


Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asn Ser 
545                 550                 555                 560 


Lys Ala Val Thr Gly Trp Gln Thr Ile Asp Ser Lys Lys Tyr Tyr Phe 
                565                 570                 575     


Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly 
            580                 585                 590         


Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp 
        595                 600                 605             


Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Ile 
    610                 615                 620                 


Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe Tyr Phe Asn 
625                 630                 635                 640 


Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe 
                645                 650                 655     


Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln 
            660                 665                 670         


Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly Lys Lys Tyr 
        675                 680                 685             


Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg Ile Ile Asn 
    690                 695                 700                 


Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala Ala Ile His 
705                 710                 715                 720 


Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr Asp Gly Ile 
                725                 730                 735     


Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe Tyr Phe Asp 
            740                 745                 750         


Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys Gly Pro Asn 
        755                 760                 765             


Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn Asn Ile Glu 
    770                 775                 780                 


Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys 
785                 790                 795                 800 


Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Gln Thr 
                805                 810                 815     


Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala 
            820                 825                 830         


Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn 
        835                 840                 845             


Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr 
    850                 855                 860                 


Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr Thr Ser Ile 
865                 870                 875                 880 


Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly 
                885                 890                 895     


Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr 
            900                 905                 910         


His Asn Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Lys Phe 
        915                 920                 925             


Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala 
    930                 935                 940                 


Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr 
945                 950                 955                 960 


Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys 
                965                 970                 975     


Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly Tyr Thr Ile 
            980                 985                 990         


Ile Ser Gly Lys His Phe Tyr Phe  Asn Thr Asp Gly Ile  Met Gln Ile 
        995                 1000                 1005             


Gly Val  Phe Lys Gly Pro Asp  Gly Phe Glu Tyr Phe  Ala Pro Ala 
    1010                 1015                 1020             


Asn Thr  Asp Ala Asn Asn Ile  Glu Gly Gln Ala Ile  Arg Tyr Gln 
    1025                 1030                 1035             


Asn Arg  Phe Leu Tyr Leu His  Asp Asn Ile Tyr Tyr  Phe Gly Asn 
    1040                 1045                 1050             


Asn Ser  Lys Ala Ala Thr Gly  Trp Val Thr Ile Asp  Gly Asn Arg 
    1055                 1060                 1065             


Tyr Tyr  Phe Glu Pro Asn Thr  Ala Met Gly Ala Asn  Gly Tyr Lys 
    1070                 1075                 1080             


Thr Ile  Asp Asn Lys Asn Phe  Tyr Phe Arg Asn Gly  Leu Pro Gln 
    1085                 1090                 1095             


Ile Gly  Val Phe Lys Gly Ser  Asn Gly Phe Glu Tyr  Phe Ala Pro 
    1100                 1105                 1110             


Ala Asn  Thr Asp Ala Asn Asn  Ile Glu Gly Gln Ala  Ile Arg Tyr 
    1115                 1120                 1125             


Gln Asn  Arg Phe Leu His Leu  Leu Gly Lys Ile Tyr  Tyr Phe Gly 
    1130                 1135                 1140             


Asn Asn  Ser Lys Ala Val Thr  Gly Trp Gln Thr Ile  Asn Gly Lys 
    1145                 1150                 1155             


Val Tyr  Tyr Phe Met Pro Asp  Thr Ala Met Ala Ala  Ala Gly Gly 
    1160                 1165                 1170             


Leu Phe  Glu Ile Asp Gly Val  Ile Tyr Phe Phe Gly  Val Asp Gly 
    1175                 1180                 1185             


Val Lys  Ala Pro Gly Ile Tyr  Pro Gly 
    1190                 1195         


<210>  14
<211>  2550
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion B Codon-optimized sequence


<220>
<221>  CDS
<222>  (1)..(2550)

<400>  14
atg acc agc att ttc gcc gaa cag act gtg gaa gtg gtg aag tcg gca         48
Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala           
1               5                   10                  15                

atc gaa acc gcg gac ggc gct ctg gat ctg tat aac aaa tat ctg gac         96
Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp           
            20                  25                  30                    

cag gta atc ccc tgg aaa acc ttc gat gaa acg atc aaa gaa ctt tcg        144
Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser           
        35                  40                  45                        

agg ttt aag cag gaa tat tcg cag gaa gcc tca gtc ctc gtc ggc gat        192
Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp           
    50                  55                  60                            

atc aaa gtg ctg ctc atg gat tct cag gat aag tat ttc gaa gca acg        240
Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr           
65                  70                  75                  80            

cag acg gtc tat gaa tgg tgt ggg gtg gtc aca cag tta ctt tcc gca        288
Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala           
                85                  90                  95                

tac atc ctt ctg ttc gat gaa tac aac gaa aaa aag gca tcc gcg cag        336
Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln           
            100                 105                 110                   

aaa gat atc tta atc agg att ctt gat gac ggt gtt aag aaa ctg aac        384
Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn           
        115                 120                 125                       

gaa gct cag aaa tcg ctg ctt aca agc tcc cag tcg ttc aac aat gcg        432
Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala           
    130                 135                 140                           

tca ggt aaa ctg tta gcg ctt gac tca cag ttg aca aat gat ttc tct        480
Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser           
145                 150                 155                 160           

gaa aag agc agt tat ttc cag tcc cag gtg gat aga ata aga aaa gag        528
Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu           
                165                 170                 175               

gca tac gcg gtg gca gcc gct ggt tcg gtg tcc ggg cca ttc ggt ctg        576
Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu           
            180                 185                 190                   

tcg att tct tat agc att gcg gct ggt gtt atc gag gga aag ctg att        624
Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile           
        195                 200                 205                       

ccg gag ctt aat aac cga ctt aag acc gtg cag aac ttc ttt act tca        672
Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser           
    210                 215                 220                           

ctc agc gcg aca gtc aag cag gcc aac aag gat atc gac gcc gcc aaa        720
Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys           
225                 230                 235                 240           

ctc aag ctg gcc aca gaa att gct gca atc ggt gag ata aag aca gag        768
Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu           
                245                 250                 255               

aca gaa acg acc cgc ttc tat gtg gac tat gat gac ctt atg ttg agt        816
Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser           
            260                 265                 270                   

ctc ctt aaa gga gcc gcc aaa aag atg ata aac acg tgc aac gag tat        864
Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr           
        275                 280                 285                       

caa caa agg cat gga aaa aag aca tta ttt gaa gtt cca gac gtt ccc        912
Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Pro           
    290                 295                 300                           

ggg aag ttt tat atc aac aac ttc ggc atg atg gtg tct ggc ttg atc        960
Gly Lys Phe Tyr Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Ile           
305                 310                 315                 320           

tac atc aac gat agc ctc tat tat ttc aag ccg ccc gtt aat aac tta       1008
Tyr Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn Leu           
                325                 330                 335               

atc aca ggc ttc gtg aca gta ggt gat gac aaa tac tat ttt aat ccg       1056
Ile Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro           
            340                 345                 350                   

atc aat gga ggc gca gca agt att ggt gaa acg ata atc gac gac aag       1104
Ile Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys           
        355                 360                 365                       

aac tat tat ttt aac caa tca gga gtg ctg caa act ggt gtg ttt tcc       1152
Asn Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser           
    370                 375                 380                           

acc gag gac ggc ttt aag tac ttc gcc ccc gcg aac acc ctg gac gaa       1200
Thr Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu           
385                 390                 395                 400           

aac ctt gag ggt gaa gcc att gac ttc act ggt aaa ctt att atc gac       1248
Asn Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp           
                405                 410                 415               

gaa aac atc tac tat ttt gat gat aac tac aga ggc gca gtg gag tgg       1296
Glu Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp           
            420                 425                 430                   

aaa gag ctg gac ggg gaa atg cat tac ttt tcc cca gag aca ggt aaa       1344
Lys Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys           
        435                 440                 445                       

gct ttc aaa ggt ctg aat cag att ggg gat tac aaa tat tac ttc aac       1392
Ala Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn           
    450                 455                 460                           

tct gac ggt gtc atg cag aag gga ttt gtg tca atc aac gat aat aag       1440
Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys           
465                 470                 475                 480           

cac tac ttt gat gac tca gga gta atg aag gtg ggc tac acg gag att       1488
His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile           
                485                 490                 495               

gac gga aaa cat ttc tat ttc gcc gaa aat ggt gaa atg cag att ggc       1536
Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly           
            500                 505                 510                   

gtt ttc aat acc gag gat ggc ttc aag tat ttt gct cat cac aat gag       1584
Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu           
        515                 520                 525                       

gat ctg gga aac gaa gaa ggc gag gaa att tcc tac tcg ggc ata ctg       1632
Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu           
    530                 535                 540                           

aat ttt aac aat aaa ata tat tat ttc gac gac agt ttt acg gcg gtt       1680
Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val           
545                 550                 555                 560           

gtt ggg tgg aag gat tta gaa gat ggt agt aaa tac tac ttc gat gag       1728
Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu           
                565                 570                 575               

gac acg gcc gaa gcc tat atc ggt ttg tcg ctg att aat gat gga cag       1776
Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln           
            580                 585                 590                   

tac tat ttt aat gac gac ggc att atg caa gtt ggg ttc gtg acc att       1824
Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile           
        595                 600                 605                       

aac gac aaa gtg ttt tat ttt tca gac tca gga att atc gag agc ggg       1872
Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly           
    610                 615                 620                           

gtt caa aac att gat gat aat tat ttt tac ata gac gat aat ggg atc       1920
Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile           
625                 630                 635                 640           

gtt cag atc ggg gtg ttc gac aca tct gac ggt tac aaa tat ttt gct       1968
Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala           
                645                 650                 655               

ccc gca aat acg gtg aac gac aac att tac ggg cag gca gtg gaa tat       2016
Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr           
            660                 665                 670                   

tcg ggt ttg gtt aga gtt ggc gag gat gtc tac tat ttt ggc gag aca       2064
Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr           
        675                 680                 685                       

tac acg att gaa acg ggg tgg att tac gat atg gag aac gaa agc gat       2112
Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp           
    690                 695                 700                           

aaa tat tac ttt aac cca gaa aca aag aag gcc tgc aaa ggt atc aat       2160
Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn           
705                 710                 715                 720           

tta atc gat gat atc aaa tac tat ttc gac gaa aag ggt atc atg cgt       2208
Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg           
                725                 730                 735               

act ggg ctg atc agc ttt gag aac aat aat tac tat ttc aat gaa aat       2256
Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn           
            740                 745                 750                   

ggg gaa atg caa ttt gga tat att aat ata gaa gat aag atg ttt tat       2304
Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr           
        755                 760                 765                       

ttc ggg gag gat ggt gtg atg cag atc ggc gtt ttc aac acc ccg gac       2352
Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp           
    770                 775                 780                           

ggg ttt aaa tat ttc gca cat cag aat aca ctg gat gag aac ttc gag       2400
Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu           
785                 790                 795                 800           

ggt gag tct att aac tac acc ggg tgg ctg gac tta gac gag aaa cgc       2448
Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg           
                805                 810                 815               

tac tat ttc aca gac gag tac att gca gct act ggt tcg gtc atc att       2496
Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile           
            820                 825                 830                   

gat ggc gag gaa tat tat ttc gac ccg gat acc gcc cag tta gtg atc       2544
Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile           
        835                 840                 845                       

tcc gag                                                               2550
Ser Glu                                                                   
    850                                                                   


<210>  15
<211>  850
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu 
            180                 185                 190         


Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Pro 
    290                 295                 300                 


Gly Lys Phe Tyr Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Ile 
305                 310                 315                 320 


Tyr Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn Leu 
                325                 330                 335     


Ile Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro 
            340                 345                 350         


Ile Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys 
        355                 360                 365             


Asn Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser 
    370                 375                 380                 


Thr Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu 
385                 390                 395                 400 


Asn Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp 
                405                 410                 415     


Glu Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp 
            420                 425                 430         


Lys Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys 
        435                 440                 445             


Ala Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn 
    450                 455                 460                 


Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys 
465                 470                 475                 480 


His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile 
                485                 490                 495     


Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly 
            500                 505                 510         


Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu 
        515                 520                 525             


Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu 
    530                 535                 540                 


Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val 
545                 550                 555                 560 


Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu 
                565                 570                 575     


Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln 
            580                 585                 590         


Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile 
        595                 600                 605             


Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly 
    610                 615                 620                 


Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile 
625                 630                 635                 640 


Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala 
                645                 650                 655     


Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr 
            660                 665                 670         


Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr 
        675                 680                 685             


Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp 
    690                 695                 700                 


Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn 
705                 710                 715                 720 


Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg 
                725                 730                 735     


Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn 
            740                 745                 750         


Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr 
        755                 760                 765             


Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp 
    770                 775                 780                 


Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu 
785                 790                 795                 800 


Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg 
                805                 810                 815     


Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile 
            820                 825                 830         


Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile 
        835                 840                 845             


Ser Glu 
    850 


<210>  16
<211>  506
<212>  DNA
<213>  Salmonella sp.

<400>  16
gcgcgccgct cgtagccctg gcagggattg gccttgctat tgccatcgcg gatgtcgcct       60

gtcttatcta ccatcataaa catcatttgc ctatggctca cgacagtata ggcaatgccg      120

ttttttatat tgctaattgt ttcgccaatc aacgcaaaag tatggcgatt gctaaagccg      180

tctccctggg cggtagatta gccttaaccg cgacggtaat gactcattca tactggagtg      240

gtagtttggg actacagcct catttattag agcgtcttaa tgatattacc tatggactaa      300

tgagttttac tcgcttcggt atggatggga tggcaatgac cggtatgcag gtcagcagcc      360

cattatatcg tttgctggct caggtaacgc cagaacaacg tgcgccggag taatcgtttt      420

caggtatata ccggatgttc attgctttct aaattttgct atgttgccag tatccttacg      480

atgtatttat tttaaggaaa agcatt                                           506


<210>  17
<211>  9290
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssaG antigen operon

<400>  17
tatccgaacg gtcaaaacgg atttttcgta ttctcccgcc gcgtcaatgc tgatttatcc       60

ctgtcttcgt ggcaaactag ccgccgaatt taatgcgagc atgccctgga ggaatacgtg      120

gataaaattt tcgtcgatga agcagtaagt gaactgcata ccattcagga catgttgcgc      180

tgggcggtaa gccgctttag cgcggcgaat atctggtatg gacacggtac cgataacccg      240

tgggatgaag cggtacaact ggtgttgccg tctctttatc tgccgctgga tattccggag      300

gatatgcgga ccgcgcggct gacgtccagc gaaagacacc gcattgtcga gcgagtgatt      360

cgtcgcatta acgagcgtat cccggtagcc tacctgacca ataaagcctg gttctgcggc      420

cacgaatttt atgttgatga gcgcgtgctg gtgccgcgtt caccgattgg cgagctgatt      480

aataaccact tcgctggcct tattagccaa cagccgaaat atattctgga tatgtgtacc      540

ggcagcggct gcatcgccat cgcctgtgct tatgctttcc cggacgcaga ggttgatgcg      600

gtcgatattt cgccggatgc gctggctgtc gccgagcata acattgaaga acacggtctt      660

atccatcacg tgacgccaat ccgttccgat ctgttccgcg atctgccgaa agttcagtac      720

gatctgattg tcactaaccc gccttatgtc gatgcggagg atatgtccga tctgccgaac      780

gaatatcgcc acgaacctga gctggggctg gcgtccggca ctgacggcct caaattgacc      840

cgccgtatcc tgggaaatgc gccggattat ctgtccgatg atggcgttct gatttgtgaa      900

gtcggaaaca gcatggtaca tctgatggag cagtatccgg atgtgccgtt cacctggctg      960

gagtttgaca acggcggcga tggcgtcttt atgttgacca aagcgcagtt gctcgcggcc     1020

cgtgaacatt tcaatattta taaagattaa aacacgcaaa cgacaacaac gataacggag     1080

ccgtgatggc aggaaacaca attggacaac tctttcgcgt aaccactttc ggcgaatcac     1140

acgggctggc gcttgggggt atcgtcgatg gcgtgccgcc cggcatcccg ttgacggagg     1200

ccgatctgca gcacgatctc gacagacgcc gccctggcac ctcgcgctat actactcagc     1260

gccgcgaacc ggaccaggta aaaattctct ccggcgtgtt tgatggcgtg acgaccggct     1320

cgagattgcc atcgcggatg tcgcctgtct tatctaccat cataaacatc atttgcctat     1380

ggctcacgac agtataggca atgccgtttt ttatattgct aattgtttcg ccaatcaacg     1440

caaaagtatg gcgattgcta aagccgtctc cctgggcggt agattagcct taaccgcgac     1500

ggtaatgact cattcatact ggagtggtag tttgggacta cagcctcatt tattagagcg     1560

tcttaatgat attacctatg gactaatgag ttttactcgc ttcggtatgg atgggatggc     1620

aatgaccggt atgcaggtca gcagcccatt atatcgtttg ctggctcagg taacgccaga     1680

acaacgtgcg ccggagtaat cgttttcagg tatataccgg atgttcattg ctttctaaat     1740

tttgctatgt tgccagtatc cttacgatgt atttatttta aggaaaagcc atatgacttc     1800

gatcttcgcc gaacagacgg ttgaggtggt aaaatcagcc atagaaaccg cggatggggc     1860

gctcgacctt tacaataagt accttgatca ggtgatcccg tggaaaacgt tcgacgagac     1920

tatcaaagaa ttatcacgat ttaagcagga atattcacag gaagcatccg tacttgttgg     1980

tgatattaaa gtcttactca tggattctca ggataagtac ttcgaggcaa cccagacggt     2040

gtacgagtgg tgtggcgttg taacacagct tctgtcggct tacattcttc tgttcgatga     2100

atataacgag aaaaaagcct ccgcccagaa agacattctg atacgcattc ttgacgatgg     2160

tgtgaagaag ctgaacgaag cacagaaatc gttattaact tcctctcagt cctttaataa     2220

cgcgtcaggc aagttactgg ctcttgattc ccagttgact aatgacttca gtgaaaaatc     2280

gtcgtatttc cagtcacaag ttgaccgtat ccgtaaagag gcttacgctg tcgctgctgc     2340

gggctcggtc agtggcccat tcggtctttc tatcagctat agcattgcag ccggagtcat     2400

agaaggcaaa ctgatcccgg agttgaacaa tcgcctgaaa accgtgcaaa atttttttac     2460

gagtttgagc gccactgtca aacaggcgaa caaggatata gatgctgcaa aactcaaatt     2520

agcgaccgaa attgccgcga taggtgaaat taagaccgaa acggagacaa cccggttcta     2580

cgtcgactac gacgacttga tgttatcatt gctgaaaggc gccgctaaaa agatgatcaa     2640

cacctgtaac gaatatcagc agcggcacgg aaaaaaaacc ctttttgagg tccctgatgt     2700

cgggcccaca tattactacg acgaagattc gaagttggtc aagggcctga taaacataaa     2760

caactcgtta ttttatttcg atcctattga atttaacctg gtgacggggt ggcagaccat     2820

aaacgggaag aagtactact ttgacatcaa taccggcgca gcattgattt catataagat     2880

aattaacggc aagcatttct actttaacaa cgatggagtc atgcaactgg gagtctttaa     2940

gggtcccgac ggcttcgaat actttgcccc agcgaacacc caaaacaaca atattgaggg     3000

gcaggcgatt gtctatcaat caaagttttt gacgctgaac ggtaagaaat actattttga     3060

taacgattcg aaagcagtca cggggtggcg gattattaac aacgaaaaat attattttaa     3120

tccaaataat gctatcgcag cagtcgggct tcaagtgatc gataataata agtactactt     3180

caatccagat acggctatta tttcaaaagg gtggcagact gtcaacggct ccaggtatta     3240

tttcgacact gatactgcta tcgctttcaa cgggtataag acaatcgatg gtaagcattt     3300

ctactttgat agcgactgcg tggttaaaat tggtgtattc agtacctcta atggatttga     3360

gtacttcgct cctgcaaaca cttacaataa caatattgaa ggtcaggcca tcgtatacca     3420

aagcaagttc ctcaccttaa atggcaaaaa gtactatttc gacaacaata gcaaagcggt     3480

caccggttgg cagaccattg atagtaaaaa atattatttt aataccaaca ctgcggaagc     3540

tgctaccgga tggcagacaa tcgacggcaa gaagtattat ttcaacacca atacagcaga     3600

agcggccaca gggtggcaaa cgatcgacgg gaagaagtac tactttaata ctaacacggc     3660

cattgctagc accggttata ccattattaa tgggaaacac ttttacttca acactgacgg     3720

cattatgcag atcggtgtat tcaaagggcc taacggcttc gaatatttcg caccggccaa     3780

tacagacgcg aacaatatag aaggacaggc gattctgtat cagaatgaat tcctgaccct     3840

gaatggtaag aaatattact tcggcagcga ttctaaggcc gtcaccgggt ggcggataat     3900

caataataaa aagtactatt tcaacccgaa taacgcgatt gcagctattc acctgtgcac     3960

gatcaacaat gataagtatt attttagcta tgatgggatc cttcaaaatg gatatattac     4020

aatagaaaga aataacttct atttcgatgc gaataatgag tctaaaatgg tgactggcgt     4080

tttcaaaggc ccaaatgggt tcgaatactt cgctccggcg aacacacaca acaacaatat     4140

tgaagggcag gcaatagtgt atcagaataa attcttgacg ctgaatggta aaaagtacta     4200

ctttgataat gattcgaaag cggtaacagg ctggcagacc atagacggca agaaatatta     4260

ctttaatctg aatactgccg aagctgcgac gggctggcaa accatagacg gaaagaaata     4320

ttattttaat ctgaacaccg cagaggccgc caccggatgg cagaccatcg acgggaagaa     4380

atactatttc aacactaata ccttcatagc gagtacgggg tatacctcga tcaatggcaa     4440

gcatttctac tttaacaccg acgggattat gcagatcggt gttttcaagg ggccgaacgg     4500

cttcgaatac ttcgctcccg caaacacaca caacaacaac atcgagggac aggctatact     4560

gtatcaaaat aaatttctta cgttaaatgg caagaagtat tattttgggt cggacagcaa     4620

agcagtgacc ggtttgcgta ccatagatgg taagaaatat tattttaata ctaacacggc     4680

agtagccgtt accggatggc agactattaa tgggaagaaa tactatttta acactaacac     4740

gagcattgcc tcgactggct acacgatcat tagcgggaaa cacttctact tcaacacgga     4800

tggtattatg cagataggtg tctttaaagg tcctgacggt tttgagtact tcgcacccgc     4860

caacaccgac gctaataaca tagaggggca agctatcagg tatcagaatc gcttccttta     4920

cctgcatgat aacatctatt acttcgggaa caacagtaag gctgctaccg ggtgggtgac     4980

aattgacggt aatcgctatt atttcgagcc taacacagca atgggagcca atggctataa     5040

gactatcgat aacaaaaatt tttactttcg gaacggtttg cctcaaatcg gggtttttaa     5100

aggatctaac ggcttcgagt actttgcccc ggcgaacacg gatgccaaca atattgaggg     5160

ccaggcgata aggtaccaga accgctttct gcatctcttg ggtaaaatct attacttcgg     5220

caacaactca aaggcggtaa caggatggca aactataaac gggaaggttt actattttat     5280

gcctgatacg gccatggctg cggcgggagg cctgttcgaa attgacggtg ttatatactt     5340

tttcggtgtg gacggtgtta aggccccagg catttacccc gggtaaggaa aagccatatg     5400

accagcattt tcgccgaaca gactgtggaa gtggtgaagt cggcaatcga aaccgcggac     5460

ggcgctctgg atctgtataa caaatatctg gaccaggtaa tcccctggaa aaccttcgat     5520

gaaacgatca aagaactttc gaggtttaag caggaatatt cgcaggaagc ctcagtcctc     5580

gtcggcgata tcaaagtgct gctcatggat tctcaggata agtatttcga agcaacgcag     5640

acggtctatg aatggtgtgg ggtggtcaca cagttacttt ccgcatacat ccttctgttc     5700

gatgaataca acgaaaaaaa ggcatccgcg cagaaagata tcttaatcag gattcttgat     5760

gacggtgtta agaaactgaa cgaagctcag aaatcgctgc ttacaagctc ccagtcgttc     5820

aacaatgcgt caggtaaact gttagcgctt gactcacagt tgacaaatga tttctctgaa     5880

aagagcagtt atttccagtc ccaggtggat agaataagaa aagaggcata cgcggtggca     5940

gccgctggtt cggtgtccgg gccattcggt ctgtcgattt cttatagcat tgcggctggt     6000

gttatcgagg gaaagctgat tccggagctt aataaccgac ttaagaccgt gcagaacttc     6060

tttacttcac tcagcgcgac agtcaagcag gccaacaagg atatcgacgc cgccaaactc     6120

aagctggcca cagaaattgc tgcaatcggt gagataaaga cagagacaga aacgacccgc     6180

ttctatgtgg actatgatga ccttatgttg agtctcctta aaggagccgc caaaaagatg     6240

ataaacacgt gcaacgagta tcaacaaagg catggaaaaa agacattatt tgaagttcca     6300

gacgttcccg ggaagtttta tatcaacaac ttcggcatga tggtgtctgg cttgatctac     6360

atcaacgata gcctctatta tttcaagccg cccgttaata acttaatcac aggcttcgtg     6420

acagtaggtg atgacaaata ctattttaat ccgatcaatg gaggcgcagc aagtattggt     6480

gaaacgataa tcgacgacaa gaactattat tttaaccaat caggagtgct gcaaactggt     6540

gtgttttcca ccgaggacgg ctttaagtac ttcgcccccg cgaacaccct ggacgaaaac     6600

cttgagggtg aagccattga cttcactggt aaacttatta tcgacgaaaa catctactat     6660

tttgatgata actacagagg cgcagtggag tggaaagagc tggacgggga aatgcattac     6720

ttttccccag agacaggtaa agctttcaaa ggtctgaatc agattgggga ttacaaatat     6780

tacttcaact ctgacggtgt catgcagaag ggatttgtgt caatcaacga taataagcac     6840

tactttgatg actcaggagt aatgaaggtg ggctacacgg agattgacgg aaaacatttc     6900

tatttcgccg aaaatggtga aatgcagatt ggcgttttca ataccgagga tggcttcaag     6960

tattttgctc atcacaatga ggatctggga aacgaagaag gcgaggaaat ttcctactcg     7020

ggcatactga attttaacaa taaaatatat tatttcgacg acagttttac ggcggttgtt     7080

gggtggaagg atttagaaga tggtagtaaa tactacttcg atgaggacac ggccgaagcc     7140

tatatcggtt tgtcgctgat taatgatgga cagtactatt ttaatgacga cggcattatg     7200

caagttgggt tcgtgaccat taacgacaaa gtgttttatt tttcagactc aggaattatc     7260

gagagcgggg ttcaaaacat tgatgataat tatttttaca tagacgataa tgggatcgtt     7320

cagatcgggg tgttcgacac atctgacggt tacaaatatt ttgctcccgc aaatacggtg     7380

aacgacaaca tttacgggca ggcagtggaa tattcgggtt tggttagagt tggcgaggat     7440

gtctactatt ttggcgagac atacacgatt gaaacggggt ggatttacga tatggagaac     7500

gaaagcgata aatattactt taacccagaa acaaagaagg cctgcaaagg tatcaattta     7560

atcgatgata tcaaatacta tttcgacgaa aagggtatca tgcgtactgg gctgatcagc     7620

tttgagaaca ataattacta tttcaatgaa aatggggaaa tgcaatttgg atatattaat     7680

atagaagata agatgtttta tttcggggag gatggtgtga tgcagatcgg cgttttcaac     7740

accccggacg ggtttaaata tttcgcacat cagaatacac tggatgagaa cttcgagggt     7800

gagtctatta actacaccgg gtggctggac ttagacgaga aacgctacta tttcacagac     7860

gagtacattg cagctactgg ttcggtcatc attgatggcg aggaatatta tttcgacccg     7920

gataccgccc agttagtgat ctccgagtaa tctagactag cctaggtcca gcattaccgt     7980

gccgggacgt acgatcaacc ggatgggtga agaggtcgag atgatcacca aagggcgcca     8040

cgatccgtgt gtggggattc gcgcagtgcc gatcgcagaa gccatgctgg cgatcgtact     8100

gatggatcac ctgctgcgcc atcgggcaca gaatgcggat gtaaagacag agattccacg     8160

ctggtaagaa atgaaaaaaa ccgcgattgc gctgctggca tggtttgtca gtagcgccag     8220

cctggcggcg acgccgtggc agaaaataac ccatcctgtc cccggcgccg cccagtctat     8280

cggtagcttt gccaacggat gcatcattgg cgccgacacg ttgccggtac agtccgataa     8340

ttatcaggtg atgcgcaccg atcagcgccg ttatttcggc cacccggatc tggtcatgtt     8400

tatccagcgg ttgagtcatc aggcgcagca acgggggctc ggaaccgtcc tgataggcga     8460

catggggatg cctgccggag gccgctttaa tggcggacac gccagtcatc agaccgggct     8520

tgatgtggat attttcttgc agttgccgaa aacgcgctgg agccaggcgc agctattgcg     8580

cccgcaggcg ttagatctgg tgtcccgcga cggtaaacat gtcgtgccgt cgcgctggtc     8640

gtcggatatc gccagtctga tcaaactggc ggcacaagac aatgacgtca cccgtatttt     8700

cgtcaatccg gctattaaac aacagctttg cctcgatgcc ggaagcgatc gtgactggct     8760

acgtaaagta cgcccctggt tccagcatcg cgcgcatatg cacgtgcgtt tacgctgccc     8820

tgccgacagc ctggagtgcg aagatcaacc tttacccccg ccgggcgatg gatgcggcgc     8880

tgaactgcaa agctggttcg aaccgccaaa acctggcacc acaaagcctg agaagaagac     8940

accgccgccg ttgccgcctt cctgccaggc gctactggat gagcatgtac tctgatggac     9000

aatttttatg atctgtttat ggtctccccg ctgctgctgg tggtgctgtt ttttgtcgcc     9060

gtactggcag gatttatcga ttctatcgcc ggaggcggag ggctgctcac tatccctgcg     9120

ctgatggccg ccgggatgtc gccggcaaac gcgttggcga ccaataaatt acaggcgtgc     9180

ggcggctccc tctcgtcttc gctctatttt attcgccgta aagtggtaaa cctggccgag     9240

caaaagctca atattctgat gacgttcatt ggctcgatga gcggcgcgct                9290


<210>  18
<211>  1197
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClyA-Toxin A repeat fusion sequence

<400>  18

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu 
            180                 185                 190         


Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Gly 
    290                 295                 300                 


Pro Thr Tyr Tyr Tyr Asp Glu Asp Ser Lys Leu Val Lys Gly Leu Ile 
305                 310                 315                 320 


Asn Ile Asn Asn Ser Leu Phe Tyr Phe Asp Pro Ile Glu Phe Asn Leu 
                325                 330                 335     


Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asp Ile 
            340                 345                 350         


Asn Thr Gly Ala Ala Leu Ile Ser Tyr Lys Ile Ile Asn Gly Lys His 
        355                 360                 365             


Phe Tyr Phe Asn Asn Asp Gly Val Met Gln Leu Gly Val Phe Lys Gly 
    370                 375                 380                 


Pro Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Gln Asn Asn Asn 
385                 390                 395                 400 


Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn 
                405                 410                 415     


Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp 
            420                 425                 430         


Arg Ile Ile Asn Asn Glu Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile 
        435                 440                 445             


Ala Ala Val Gly Leu Gln Val Ile Asp Asn Asn Lys Tyr Tyr Phe Asn 
    450                 455                 460                 


Pro Asp Thr Ala Ile Ile Ser Lys Gly Trp Gln Thr Val Asn Gly Ser 
465                 470                 475                 480 


Arg Tyr Tyr Phe Asp Thr Asp Thr Ala Ile Ala Phe Asn Gly Tyr Lys 
                485                 490                 495     


Thr Ile Asp Gly Lys His Phe Tyr Phe Asp Ser Asp Cys Val Val Lys 
            500                 505                 510         


Ile Gly Val Phe Ser Thr Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala 
        515                 520                 525             


Asn Thr Tyr Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser 
    530                 535                 540                 


Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asn Ser 
545                 550                 555                 560 


Lys Ala Val Thr Gly Trp Gln Thr Ile Asp Ser Lys Lys Tyr Tyr Phe 
                565                 570                 575     


Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly 
            580                 585                 590         


Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp 
        595                 600                 605             


Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Ile 
    610                 615                 620                 


Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe Tyr Phe Asn 
625                 630                 635                 640 


Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe 
                645                 650                 655     


Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln 
            660                 665                 670         


Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly Lys Lys Tyr 
        675                 680                 685             


Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg Ile Ile Asn 
    690                 695                 700                 


Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala Ala Ile His 
705                 710                 715                 720 


Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr Asp Gly Ile 
                725                 730                 735     


Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe Tyr Phe Asp 
            740                 745                 750         


Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys Gly Pro Asn 
        755                 760                 765             


Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn Asn Ile Glu 
    770                 775                 780                 


Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys 
785                 790                 795                 800 


Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Gln Thr 
                805                 810                 815     


Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala 
            820                 825                 830         


Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn 
        835                 840                 845             


Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr 
    850                 855                 860                 


Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr Thr Ser Ile 
865                 870                 875                 880 


Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly 
                885                 890                 895     


Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr 
            900                 905                 910         


His Asn Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Lys Phe 
        915                 920                 925             


Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala 
    930                 935                 940                 


Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr 
945                 950                 955                 960 


Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys 
                965                 970                 975     


Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly Tyr Thr Ile 
            980                 985                 990         


Ile Ser Gly Lys His Phe Tyr Phe  Asn Thr Asp Gly Ile  Met Gln Ile 
        995                 1000                 1005             


Gly Val  Phe Lys Gly Pro Asp  Gly Phe Glu Tyr Phe  Ala Pro Ala 
    1010                 1015                 1020             


Asn Thr  Asp Ala Asn Asn Ile  Glu Gly Gln Ala Ile  Arg Tyr Gln 
    1025                 1030                 1035             


Asn Arg  Phe Leu Tyr Leu His  Asp Asn Ile Tyr Tyr  Phe Gly Asn 
    1040                 1045                 1050             


Asn Ser  Lys Ala Ala Thr Gly  Trp Val Thr Ile Asp  Gly Asn Arg 
    1055                 1060                 1065             


Tyr Tyr  Phe Glu Pro Asn Thr  Ala Met Gly Ala Asn  Gly Tyr Lys 
    1070                 1075                 1080             


Thr Ile  Asp Asn Lys Asn Phe  Tyr Phe Arg Asn Gly  Leu Pro Gln 
    1085                 1090                 1095             


Ile Gly  Val Phe Lys Gly Ser  Asn Gly Phe Glu Tyr  Phe Ala Pro 
    1100                 1105                 1110             


Ala Asn  Thr Asp Ala Asn Asn  Ile Glu Gly Gln Ala  Ile Arg Tyr 
    1115                 1120                 1125             


Gln Asn  Arg Phe Leu His Leu  Leu Gly Lys Ile Tyr  Tyr Phe Gly 
    1130                 1135                 1140             


Asn Asn  Ser Lys Ala Val Thr  Gly Trp Gln Thr Ile  Asn Gly Lys 
    1145                 1150                 1155             


Val Tyr  Tyr Phe Met Pro Asp  Thr Ala Met Ala Ala  Ala Gly Gly 
    1160                 1165                 1170             


Leu Phe  Glu Ile Asp Gly Val  Ile Tyr Phe Phe Gly  Val Asp Gly 
    1175                 1180                 1185             


Val Lys  Ala Pro Gly Ile Tyr  Pro Gly 
    1190                 1195         


<210>  19
<211>  850
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClyA-Toxin B repeat fusion sequence

<400>  19

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu 
            180                 185                 190         


Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Pro 
    290                 295                 300                 


Gly Lys Phe Tyr Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Ile 
305                 310                 315                 320 


Tyr Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn Leu 
                325                 330                 335     


Ile Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro 
            340                 345                 350         


Ile Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys 
        355                 360                 365             


Asn Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser 
    370                 375                 380                 


Thr Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu 
385                 390                 395                 400 


Asn Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp 
                405                 410                 415     


Glu Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp 
            420                 425                 430         


Lys Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys 
        435                 440                 445             


Ala Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn 
    450                 455                 460                 


Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys 
465                 470                 475                 480 


His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile 
                485                 490                 495     


Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly 
            500                 505                 510         


Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu 
        515                 520                 525             


Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu 
    530                 535                 540                 


Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val 
545                 550                 555                 560 


Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu 
                565                 570                 575     


Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln 
            580                 585                 590         


Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile 
        595                 600                 605             


Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly 
    610                 615                 620                 


Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile 
625                 630                 635                 640 


Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala 
                645                 650                 655     


Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr 
            660                 665                 670         


Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr 
        675                 680                 685             


Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp 
    690                 695                 700                 


Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn 
705                 710                 715                 720 


Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg 
                725                 730                 735     


Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn 
            740                 745                 750         


Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr 
        755                 760                 765             


Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp 
    770                 775                 780                 


Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu 
785                 790                 795                 800 


Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg 
                805                 810                 815     


Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile 
            820                 825                 830         


Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile 
        835                 840                 845             


Ser Glu 
    850 


<210>  20
<211>  8356
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClyA-Toxin A repeats-Toxin B repeats in aroC and under the 
       control of an ssaG promoter

<400>  20
aacggtcaaa acggattttt cgtattctcc cgccgcgtca atgctgattt atccctgtct       60

tcgtggcaaa ctagccgccg aatttaatgc gagcatgccc tggaggaata cgtggataaa      120

attttcgtcg atgaagcagt aagtgaactg cataccattc aggacatgtt gcgctgggcg      180

gtaagccgct ttagcgcggc gaatatctgg tatggacacg gtaccgataa cccgtgggat      240

gaagcggtac aactggtgtt gccgtctctt tatctgccgc tggatattcc ggaggatatg      300

cggaccgcgc ggctgacgtc cagcgaaaga caccgcattg tcgagcgagt gattcgtcgc      360

attaacgagc gtatcccggt agcctacctg accaataaag cctggttctg cggccacgaa      420

ttttatgttg atgagcgcgt gctggtgccg cgttcaccga ttggcgagct gattaataac      480

cacttcgctg gccttattag ccaacagccg aaatatattc tggatatgtg taccggcagc      540

ggctgcatcg ccatcgcctg tgcttatgct ttcccggacg cagaggttga tgcggtcgat      600

atttcgccgg atgcgctggc tgtcgccgag cataacattg aagaacacgg tcttatccat      660

cacgtgacgc caatccgttc cgatctgttc cgcgatctgc cgaaagttca gtacgatctg      720

attgtcacta acccgcctta tgtcgatgcg gaggatatgt ccgatctgcc gaacgaatat      780

cgccacgaac ctgagctggg gctggcgtcc ggcactgacg gcctcaaatt gacccgccgt      840

atcctgggaa atgcgccgga ttatctgtcc gatgatggcg ttctgatttg tgaagtcgga      900

aacagcatgg tacatctgat ggagcagtat ccggatgtgc cgttcacctg gctggagttt      960

gacaacggcg gcgatggcgt ctttatgttg accaaagcgc agttgctcgc ggcccgtgaa     1020

catttcaata tttataaaga ttaaaacacg caaacgacaa caacgataac ggagccgtga     1080

tggcaggaaa cacaattgga caactctttc gcgtaaccac tttcggcgaa tcacacgggc     1140

tggcgcttgg gggtatcgtc gatggcgtgc cgcccggcat cccgttgacg gaggccgatc     1200

tgcagcacga tctcgacaga cgccgccctg gcacctcgcg ctatactact cagcgccgcg     1260

aaccggacca ggtaaaaatt ctctccggcg tgtttgatgg cgtgacgacc ggctcgagat     1320

tgccatcgcg gatgtcgcct gtcttatcta ccatcataaa catcatttgc ctatggctca     1380

cgacagtata ggcaatgccg ttttttatat tgctaattgt ttcgccaatc aacgcaaaag     1440

tatggcgatt gctaaagccg tctccctggg cggtagatta gccttaaccg cgacggtaat     1500

gactcattca tactggagtg gtagtttggg actacagcct catttattag agcgtcttaa     1560

tgatattacc tatggactaa tgagttttac tcgcttcggt atggatggga tggcaatgac     1620

cggtatgcag gtcagcagcc cattatatcg tttgctggct caggtaacgc cagaacaacg     1680

tgcgccggag taatcgtttt caggtatata ccggatgttc attgctttct aaattttgct     1740

atgttgccag tatccttacg atgtatttat tttaaggaaa agccatatga cttcgatctt     1800

cgccgaacag acggttgagg tggtaaaatc agccatagaa accgcggatg gggcgctcga     1860

cctttacaat aagtaccttg atcaggtgat cccgtggaaa acgttcgacg agactatcaa     1920

agaattatca cgatttaagc aggaatattc acaggaagca tccgtacttg ttggtgatat     1980

taaagtctta ctcatggatt ctcaggataa gtacttcgag gcaacccaga cggtgtacga     2040

gtggtgtggc gttgtaacac agcttctgtc ggcttacatt cttctgttcg atgaatataa     2100

cgagaaaaaa gcctccgccc agaaagacat tctgatacgc attcttgacg atggtgtgaa     2160

gaagctgaac gaagcacaga aatcgttatt aacttcctct cagtccttta ataacgcgtc     2220

aggcaagtta ctggctcttg attcccagtt gactaatgac ttcagtgaaa aatcgtcgta     2280

tttccagtca caagttgacc gtatccgtaa agaggcttac gctgtcgctg ctgcgggctc     2340

ggtcagtggc ccattcggtc tttctatcag ctatagcatt gcagccggag tcatagaagg     2400

caaactgatc ccggagttga acaatcgcct gaaaaccgtg caaaattttt ttacgagttt     2460

gagcgccact gtcaaacagg cgaacaagga tatagatgct gcaaaactca aattagcgac     2520

cgaaattgcc gcgataggtg aaattaagac cgaaacggag acaacccggt tctacgtcga     2580

ctacgacgac ttgatgttat cattgctgaa aggcgccgct aaaaagatga tcaacacctg     2640

taacgaatat cagcagcggc acggaaaaaa aacccttttt gaggtccctg atgtcgggcc     2700

cacatattac tacgacgaag attcgaagtt ggtcaagggc ctgataaaca taaacaactc     2760

gttattttat ttcgatccta ttgaatttaa cctggtgacg gggtggcaga ccataaacgg     2820

gaagaagtac tactttgaca tcaataccgg cgcagcattg atttcatata agataattaa     2880

cggcaagcat ttctacttta acaacgatgg agtcatgcaa ctgggagtct ttaagggtcc     2940

cgacggcttc gaatactttg ccccagcgaa cacccaaaac aacaatattg aggggcaggc     3000

gattgtctat caatcaaagt ttttgacgct gaacggtaag aaatactatt ttgataacga     3060

ttcgaaagca gtcacggggt ggcggattat taacaacgaa aaatattatt ttaatccaaa     3120

taatgctatc gcagcagtcg ggcttcaagt gatcgataat aataagtact acttcaatcc     3180

agatacggct attatttcaa aagggtggca gactgtcaac ggctccaggt attatttcga     3240

cactgatact gctatcgctt tcaacgggta taagacaatc gatggtaagc atttctactt     3300

tgatagcgac tgcgtggtta aaattggtgt attcagtacc tctaatggat ttgagtactt     3360

cgctcctgca aacacttaca ataacaatat tgaaggtcag gccatcgtat accaaagcaa     3420

gttcctcacc ttaaatggca aaaagtacta tttcgacaac aatagcaaag cggtcaccgg     3480

ttggcagacc attgatagta aaaaatatta ttttaatacc aacactgcgg aagctgctac     3540

cggatggcag acaatcgacg gcaagaagta ttatttcaac accaatacag cagaagcggc     3600

cacagggtgg caaacgatcg acgggaagaa gtactacttt aatactaaca cggccattgc     3660

tagcaccggt tataccatta ttaatgggaa acacttttac ttcaacactg acggcattat     3720

gcagatcggt gtattcaaag ggcctaacgg cttcgaatat ttcgcaccgg ccaatacaga     3780

cgcgaacaat atagaaggac aggcgattct gtatcagaat gaattcctga ccctgaatgg     3840

taagaaatat tacttcggca gcgattctaa ggccgtcacc gggtggcgga taatcaataa     3900

taaaaagtac tatttcaacc cgaataacgc gattgcagct attcacctgt gcacgatcaa     3960

caatgataag tattatttta gctatgatgg gatccttcaa aatggatata ttacaataga     4020

aagaaataac ttctatttcg atgcgaataa tgagtctaaa atggtgactg gcgttttcaa     4080

aggcccaaat gggttcgaat acttcgctcc ggcgaacaca cacaacaaca atattgaagg     4140

gcaggcaata gtgtatcaga ataaattctt gacgctgaat ggtaaaaagt actactttga     4200

taatgattcg aaagcggtaa caggctggca gaccatagac ggcaagaaat attactttaa     4260

tctgaatact gccgaagctg cgacgggctg gcaaaccata gacggaaaga aatattattt     4320

taatctgaac accgcagagg ccgccaccgg atggcagacc atcgacggga agaaatacta     4380

tttcaacact aataccttca tagcgagtac ggggtatacc tcgatcaatg gcaagcattt     4440

ctactttaac accgacggga ttatgcagat cggtgttttc aaggggccga acggcttcga     4500

atacttcgct cccgcaaaca cacacaacaa caacatcgag ggacaggcta tactgtatca     4560

aaataaattt cttacgttaa atggcaagaa gtattatttt gggtcggaca gcaaagcagt     4620

gaccggtttg cgtaccatag atggtaagaa atattatttt aatactaaca cggcagtagc     4680

cgttaccgga tggcagacta ttaatgggaa gaaatactat tttaacacta acacgagcat     4740

tgcctcgact ggctacacga tcattagcgg gaaacacttc tacttcaaca cggatggtat     4800

tatgcagata ggtgtcttta aaggtcctga cggttttgag tacttcgcac ccgccaacac     4860

cgacgctaat aacatagagg ggcaagctat caggtatcag aatcgcttcc tttacctgca     4920

tgataacatc tattacttcg ggaacaacag taaggctgct accgggtggg tgacaattga     4980

cggtaatcgc tattatttcg agcctaacac agcaatggga gccaatggct ataagactat     5040

cgataacaaa aatttttact ttcggaacgg tttgcctcaa atcggggttt ttaaaggatc     5100

taacggcttc gagtactttg ccccggcgaa cacggatgcc aacaatattg agggccaggc     5160

gataaggtac cagaaccgct ttctgcatct cttgggtaaa atctattact tcggcaacaa     5220

ctcaaaggcg gtaacaggat ggcaaactat aaacgggaag gtttactatt ttatgcctga     5280

tacggccatg gctgcggcgg gaggcctgtt cgaaattgac ggtgttatat actttttcgg     5340

tgtggacggt gttaaggccc caggcattta ccccgggaag ttttatatca acaacttcgg     5400

catgatggtg tctggcttga tctacatcaa cgatagcctc tattatttca agccgcccgt     5460

taataactta atcacaggct tcgtgacagt aggtgatgac aaatactatt ttaatccgat     5520

caatggaggc gcagcaagta ttggtgaaac gataatcgac gacaagaact attattttaa     5580

ccaatcagga gtgctgcaaa ctggtgtgtt ttccaccgag gacggcttta agtacttcgc     5640

ccccgcgaac accctggacg aaaaccttga gggtgaagcc attgacttca ctggtaaact     5700

tattatcgac gaaaacatct actattttga tgataactac agaggcgcag tggagtggaa     5760

agagctggac ggggaaatgc attacttttc cccagagaca ggtaaagctt tcaaaggtct     5820

gaatcagatt ggggattaca aatattactt caactctgac ggtgtcatgc agaagggatt     5880

tgtgtcaatc aacgataata agcactactt tgatgactca ggagtaatga aggtgggcta     5940

cacggagatt gacggaaaac atttctattt cgccgaaaat ggtgaaatgc agattggcgt     6000

tttcaatacc gaggatggct tcaagtattt tgctcatcac aatgaggatc tgggaaacga     6060

agaaggcgag gaaatttcct actcgggcat actgaatttt aacaataaaa tatattattt     6120

cgacgacagt tttacggcgg ttgttgggtg gaaggattta gaagatggta gtaaatacta     6180

cttcgatgag gacacggccg aagcctatat cggtttgtcg ctgattaatg atggacagta     6240

ctattttaat gacgacggca ttatgcaagt tgggttcgtg accattaacg acaaagtgtt     6300

ttatttttca gactcaggaa ttatcgagag cggggttcaa aacattgatg ataattattt     6360

ttacatagac gataatggga tcgttcagat cggggtgttc gacacatctg acggttacaa     6420

atattttgct cccgcaaata cggtgaacga caacatttac gggcaggcag tggaatattc     6480

gggtttggtt agagttggcg aggatgtcta ctattttggc gagacataca cgattgaaac     6540

ggggtggatt tacgatatgg agaacgaaag cgataaatat tactttaacc cagaaacaaa     6600

gaaggcctgc aaaggtatca atttaatcga tgatatcaaa tactatttcg acgaaaaggg     6660

tatcatgcgt actgggctga tcagctttga gaacaataat tactatttca atgaaaatgg     6720

ggaaatgcaa tttggatata ttaatataga agataagatg ttttatttcg gggaggatgg     6780

tgtgatgcag atcggcgttt tcaacacccc ggacgggttt aaatatttcg cacatcagaa     6840

tacactggat gagaacttcg agggtgagtc tattaactac accgggtggc tggacttaga     6900

cgagaaacgc tactatttca cagacgagta cattgcagct actggttcgg tcatcattga     6960

tggcgaggaa tattatttcg acccggatac cgcccagtta gtgatctccg agtaatctag     7020

actagcctag gtccagcatt accgtgccgg gacgtacgat caaccggatg ggtgaagagg     7080

tcgagatgat caccaaaggg cgccacgatc cgtgtgtggg gattcgcgca gtgccgatcg     7140

cagaagccat gctggcgatc gtactgatgg atcacctgct gcgccatcgg gcacagaatg     7200

cggatgtaaa gacagagatt ccacgctggt aagaaatgaa aaaaaccgcg attgcgctgc     7260

tggcatggtt tgtcagtagc gccagcctgg cggcgacgcc gtggcagaaa ataacccatc     7320

ctgtccccgg cgccgcccag tctatcggta gctttgccaa cggatgcatc attggcgccg     7380

acacgttgcc ggtacagtcc gataattatc aggtgatgcg caccgatcag cgccgttatt     7440

tcggccaccc ggatctggtc atgtttatcc agcggttgag tcatcaggcg cagcaacggg     7500

ggctcggaac cgtcctgata ggcgacatgg ggatgcctgc cggaggccgc tttaatggcg     7560

gacacgccag tcatcagacc gggcttgatg tggatatttt cttgcagttg ccgaaaacgc     7620

gctggagcca ggcgcagcta ttgcgcccgc aggcgttaga tctggtgtcc cgcgacggta     7680

aacatgtcgt gccgtcgcgc tggtcgtcgg atatcgccag tctgatcaaa ctggcggcac     7740

aagacaatga cgtcacccgt attttcgtca atccggctat taaacaacag ctttgcctcg     7800

atgccggaag cgatcgtgac tggctacgta aagtacgccc ctggttccag catcgcgcgc     7860

atatgcacgt gcgtttacgc tgccctgccg acagcctgga gtgcgaagat caacctttac     7920

ccccgccggg cgatggatgc ggcgctgaac tgcaaagctg gttcgaaccg ccaaaacctg     7980

gcaccacaaa gcctgagaag aagacaccgc cgccgttgcc gccttcctgc caggcgctac     8040

tggatgagca tgtactctga tggacaattt ttatgatctg tttatggtct ccccgctgct     8100

gctggtggtg ctgttttttg tcgccgtact ggcaggattt atcgattcta tcgccggagg     8160

cggagggctg ctcactatcc ctgcgctgat ggccgccggg atgtcgccgg caaacgcgtt     8220

ggcgaccaat aaattacagg cgtgcggcgg ctccctctcg tcttcgctct attttattcg     8280

ccgtaaagtg gtaaacctgg ccgagcaaaa gctcaatatt ctgatgacgt tcattggctc     8340

gatgagcggc gcgctg                                                     8356


<210>  21
<211>  1742
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClyA-Toxin A repeats-Toxin B repeats

<400>  21

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu 
            180                 185                 190         


Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Gly 
    290                 295                 300                 


Pro Thr Tyr Tyr Tyr Asp Glu Asp Ser Lys Leu Val Lys Gly Leu Ile 
305                 310                 315                 320 


Asn Ile Asn Asn Ser Leu Phe Tyr Phe Asp Pro Ile Glu Phe Asn Leu 
                325                 330                 335     


Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asp Ile 
            340                 345                 350         


Asn Thr Gly Ala Ala Leu Ile Ser Tyr Lys Ile Ile Asn Gly Lys His 
        355                 360                 365             


Phe Tyr Phe Asn Asn Asp Gly Val Met Gln Leu Gly Val Phe Lys Gly 
    370                 375                 380                 


Pro Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Gln Asn Asn Asn 
385                 390                 395                 400 


Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn 
                405                 410                 415     


Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp 
            420                 425                 430         


Arg Ile Ile Asn Asn Glu Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile 
        435                 440                 445             


Ala Ala Val Gly Leu Gln Val Ile Asp Asn Asn Lys Tyr Tyr Phe Asn 
    450                 455                 460                 


Pro Asp Thr Ala Ile Ile Ser Lys Gly Trp Gln Thr Val Asn Gly Ser 
465                 470                 475                 480 


Arg Tyr Tyr Phe Asp Thr Asp Thr Ala Ile Ala Phe Asn Gly Tyr Lys 
                485                 490                 495     


Thr Ile Asp Gly Lys His Phe Tyr Phe Asp Ser Asp Cys Val Val Lys 
            500                 505                 510         


Ile Gly Val Phe Ser Thr Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala 
        515                 520                 525             


Asn Thr Tyr Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser 
    530                 535                 540                 


Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asn Ser 
545                 550                 555                 560 


Lys Ala Val Thr Gly Trp Gln Thr Ile Asp Ser Lys Lys Tyr Tyr Phe 
                565                 570                 575     


Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly 
            580                 585                 590         


Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp 
        595                 600                 605             


Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Ile 
    610                 615                 620                 


Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe Tyr Phe Asn 
625                 630                 635                 640 


Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe 
                645                 650                 655     


Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln 
            660                 665                 670         


Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly Lys Lys Tyr 
        675                 680                 685             


Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg Ile Ile Asn 
    690                 695                 700                 


Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala Ala Ile His 
705                 710                 715                 720 


Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr Asp Gly Ile 
                725                 730                 735     


Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe Tyr Phe Asp 
            740                 745                 750         


Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys Gly Pro Asn 
        755                 760                 765             


Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn Asn Ile Glu 
    770                 775                 780                 


Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys 
785                 790                 795                 800 


Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Gln Thr 
                805                 810                 815     


Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala 
            820                 825                 830         


Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn 
        835                 840                 845             


Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr 
    850                 855                 860                 


Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr Thr Ser Ile 
865                 870                 875                 880 


Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly 
                885                 890                 895     


Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr 
            900                 905                 910         


His Asn Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Lys Phe 
        915                 920                 925             


Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala 
    930                 935                 940                 


Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr 
945                 950                 955                 960 


Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys 
                965                 970                 975     


Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly Tyr Thr Ile 
            980                 985                 990         


Ile Ser Gly Lys His Phe Tyr Phe  Asn Thr Asp Gly Ile  Met Gln Ile 
        995                 1000                 1005             


Gly Val  Phe Lys Gly Pro Asp  Gly Phe Glu Tyr Phe  Ala Pro Ala 
    1010                 1015                 1020             


Asn Thr  Asp Ala Asn Asn Ile  Glu Gly Gln Ala Ile  Arg Tyr Gln 
    1025                 1030                 1035             


Asn Arg  Phe Leu Tyr Leu His  Asp Asn Ile Tyr Tyr  Phe Gly Asn 
    1040                 1045                 1050             


Asn Ser  Lys Ala Ala Thr Gly  Trp Val Thr Ile Asp  Gly Asn Arg 
    1055                 1060                 1065             


Tyr Tyr  Phe Glu Pro Asn Thr  Ala Met Gly Ala Asn  Gly Tyr Lys 
    1070                 1075                 1080             


Thr Ile  Asp Asn Lys Asn Phe  Tyr Phe Arg Asn Gly  Leu Pro Gln 
    1085                 1090                 1095             


Ile Gly  Val Phe Lys Gly Ser  Asn Gly Phe Glu Tyr  Phe Ala Pro 
    1100                 1105                 1110             


Ala Asn  Thr Asp Ala Asn Asn  Ile Glu Gly Gln Ala  Ile Arg Tyr 
    1115                 1120                 1125             


Gln Asn  Arg Phe Leu His Leu  Leu Gly Lys Ile Tyr  Tyr Phe Gly 
    1130                 1135                 1140             


Asn Asn  Ser Lys Ala Val Thr  Gly Trp Gln Thr Ile  Asn Gly Lys 
    1145                 1150                 1155             


Val Tyr  Tyr Phe Met Pro Asp  Thr Ala Met Ala Ala  Ala Gly Gly 
    1160                 1165                 1170             


Leu Phe  Glu Ile Asp Gly Val  Ile Tyr Phe Phe Gly  Val Asp Gly 
    1175                 1180                 1185             


Val Lys  Ala Pro Gly Ile Tyr  Pro Gly Lys Phe Tyr  Ile Asn Asn 
    1190                 1195                 1200             


Phe Gly  Met Met Val Ser Gly  Leu Ile Tyr Ile Asn  Asp Ser Leu 
    1205                 1210                 1215             


Tyr Tyr  Phe Lys Pro Pro Val  Asn Asn Leu Ile Thr  Gly Phe Val 
    1220                 1225                 1230             


Thr Val  Gly Asp Asp Lys Tyr  Tyr Phe Asn Pro Ile  Asn Gly Gly 
    1235                 1240                 1245             


Ala Ala  Ser Ile Gly Glu Thr  Ile Ile Asp Asp Lys  Asn Tyr Tyr 
    1250                 1255                 1260             


Phe Asn  Gln Ser Gly Val Leu  Gln Thr Gly Val Phe  Ser Thr Glu 
    1265                 1270                 1275             


Asp Gly  Phe Lys Tyr Phe Ala  Pro Ala Asn Thr Leu  Asp Glu Asn 
    1280                 1285                 1290             


Leu Glu  Gly Glu Ala Ile Asp  Phe Thr Gly Lys Leu  Ile Ile Asp 
    1295                 1300                 1305             


Glu Asn  Ile Tyr Tyr Phe Asp  Asp Asn Tyr Arg Gly  Ala Val Glu 
    1310                 1315                 1320             


Trp Lys  Glu Leu Asp Gly Glu  Met His Tyr Phe Ser  Pro Glu Thr 
    1325                 1330                 1335             


Gly Lys  Ala Phe Lys Gly Leu  Asn Gln Ile Gly Asp  Tyr Lys Tyr 
    1340                 1345                 1350             


Tyr Phe  Asn Ser Asp Gly Val  Met Gln Lys Gly Phe  Val Ser Ile 
    1355                 1360                 1365             


Asn Asp  Asn Lys His Tyr Phe  Asp Asp Ser Gly Val  Met Lys Val 
    1370                 1375                 1380             


Gly Tyr  Thr Glu Ile Asp Gly  Lys His Phe Tyr Phe  Ala Glu Asn 
    1385                 1390                 1395             


Gly Glu  Met Gln Ile Gly Val  Phe Asn Thr Glu Asp  Gly Phe Lys 
    1400                 1405                 1410             


Tyr Phe  Ala His His Asn Glu  Asp Leu Gly Asn Glu  Glu Gly Glu 
    1415                 1420                 1425             


Glu Ile  Ser Tyr Ser Gly Ile  Leu Asn Phe Asn Asn  Lys Ile Tyr 
    1430                 1435                 1440             


Tyr Phe  Asp Asp Ser Phe Thr  Ala Val Val Gly Trp  Lys Asp Leu 
    1445                 1450                 1455             


Glu Asp  Gly Ser Lys Tyr Tyr  Phe Asp Glu Asp Thr  Ala Glu Ala 
    1460                 1465                 1470             


Tyr Ile  Gly Leu Ser Leu Ile  Asn Asp Gly Gln Tyr  Tyr Phe Asn 
    1475                 1480                 1485             


Asp Asp  Gly Ile Met Gln Val  Gly Phe Val Thr Ile  Asn Asp Lys 
    1490                 1495                 1500             


Val Phe  Tyr Phe Ser Asp Ser  Gly Ile Ile Glu Ser  Gly Val Gln 
    1505                 1510                 1515             


Asn Ile  Asp Asp Asn Tyr Phe  Tyr Ile Asp Asp Asn  Gly Ile Val 
    1520                 1525                 1530             


Gln Ile  Gly Val Phe Asp Thr  Ser Asp Gly Tyr Lys  Tyr Phe Ala 
    1535                 1540                 1545             


Pro Ala  Asn Thr Val Asn Asp  Asn Ile Tyr Gly Gln  Ala Val Glu 
    1550                 1555                 1560             


Tyr Ser  Gly Leu Val Arg Val  Gly Glu Asp Val Tyr  Tyr Phe Gly 
    1565                 1570                 1575             


Glu Thr  Tyr Thr Ile Glu Thr  Gly Trp Ile Tyr Asp  Met Glu Asn 
    1580                 1585                 1590             


Glu Ser  Asp Lys Tyr Tyr Phe  Asn Pro Glu Thr Lys  Lys Ala Cys 
    1595                 1600                 1605             


Lys Gly  Ile Asn Leu Ile Asp  Asp Ile Lys Tyr Tyr  Phe Asp Glu 
    1610                 1615                 1620             


Lys Gly  Ile Met Arg Thr Gly  Leu Ile Ser Phe Glu  Asn Asn Asn 
    1625                 1630                 1635             


Tyr Tyr  Phe Asn Glu Asn Gly  Glu Met Gln Phe Gly  Tyr Ile Asn 
    1640                 1645                 1650             


Ile Glu  Asp Lys Met Phe Tyr  Phe Gly Glu Asp Gly  Val Met Gln 
    1655                 1660                 1665             


Ile Gly  Val Phe Asn Thr Pro  Asp Gly Phe Lys Tyr  Phe Ala His 
    1670                 1675                 1680             


Gln Asn  Thr Leu Asp Glu Asn  Phe Glu Gly Glu Ser  Ile Asn Tyr 
    1685                 1690                 1695             


Thr Gly  Trp Leu Asp Leu Asp  Glu Lys Arg Tyr Tyr  Phe Thr Asp 
    1700                 1705                 1710             


Glu Tyr  Ile Ala Ala Thr Gly  Ser Val Ile Ile Asp  Gly Glu Glu 
    1715                 1720                 1725             


Tyr Tyr  Phe Asp Pro Asp Thr  Ala Gln Leu Val Ile  Ser Glu 
    1730                 1735                 1740         


<210>  22
<211>  7016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClyA-Toxin A repeat aroC and under the control of an ssaG 
       promoter

<400>  22
cgcccccagt tcctgcttgg cctgaagctg cgttaagccg tgtaaatcca gaaacagttc       60

tggcgaatag tcgccgcggc gcattttttt tagctcgaaa tggctgacat cttcacggac      120

atactttacc ggcccttccg tattgagcag cggctggaac tcatcagaaa aatagtggct      180

ggcgtccgcc tgttcctgaa ttaaccttcg ggtagggact tccgtgattt tcttgcgcag      240

tggccgatgg acgatagtat cctgtttaat ctttcgggtg ccgaccatca gctgacggaa      300

cagcgcctgg tcctcctcgc tgagcgatgt tttctttttc attcgtgggt ctcgtctgat      360

cttttgcctt agtttacccg acccggagga tatccgaacg gtcaaaacgg atttttcgta      420

ttctcccgcc gcgtcaatgc tgatttatcc ctgtcttcgt ggcaaactag ccgccgaatt      480

taatgcgagc atgccctgga ggaatacgtg gataaaattt tcgtcgatga agcagtaagt      540

gaactgcata ccattcagga catgttgcgc tgggcggtaa gccgctttag cgcggcgaat      600

atctggtatg gacacggtac cgataacccg tgggatgaag cggtacaact ggtgttgccg      660

tctctttatc tgccgctgga tattccggag gatatgcgga ccgcgcggct gacgtccagc      720

gaaagacacc gcattgtcga gcgagtgatt cgtcgcatta acgagcgtat cccggtagcc      780

tacctgacca ataaagcctg gttctgcggc cacgaatttt atgttgatga gcgcgtgctg      840

gtgccgcgtt caccgattgg cgagctgatt aataaccact tcgctggcct tattagccaa      900

cagccgaaat atattctgga tatgtgtacc ggcagcggct gcatcgccat cgcctgtgct      960

tatgctttcc cggacgcaga ggttgatgcg gtcgatattt cgccggatgc gctggctgtc     1020

gccgagcata acattgaaga acacggtctt atccatcacg tgacgccaat ccgttccgat     1080

ctgttccgcg atctgccgaa agttcagtac gatctgattg tcactaaccc gccttatgtc     1140

gatgcggagg atatgtccga tctgccgaac gaatatcgcc acgaacctga gctggggctg     1200

gcgtccggca ctgacggcct caaattgacc cgccgtatcc tgggaaatgc gccggattat     1260

ctgtccgatg atggcgttct gatttgtgaa gtcggaaaca gcatggtaca tctgatggag     1320

cagtatccgg atgtgccgtt cacctggctg gagtttgaca acggcggcga tggcgtcttt     1380

atgttgacca aagcgcagtt gctcgcggcc cgtgaacatt tcaatattta taaagattaa     1440

aacacgcaaa cgacaacaac gataacggag ccgtgatggc aggaaacaca attggacaac     1500

tctttcgcgt aaccactttc ggcgaatcac acgggctggc gcttgggggt atcgtcgatg     1560

gcgtgccgcc cggcatcccg ttgacggagg ccgatctgca gcacgatctc gacagacgcc     1620

gccctggcac ctcgcgctat actactcagc gccgcgaacc ggaccaggta aaaattctct     1680

ccggcgtgtt tgatggcgtg acgaccggct cgagattgcc atcgcggatg tcgcctgtct     1740

tatctaccat cataaacatc atttgcctat ggctcacgac agtataggca atgccgtttt     1800

ttatattgct aattgtttcg ccaatcaacg caaaagtatg gcgattgcta aagccgtctc     1860

cctgggcggt agattagcct taaccgcgac ggtaatgact cattcatact ggagtggtag     1920

tttgggacta cagcctcatt tattagagcg tcttaatgat attacctatg gactaatgag     1980

ttttactcgc ttcggtatgg atgggatggc aatgaccggt atgcaggtca gcagcccatt     2040

atatcgtttg ctggctcagg taacgccaga acaacgtgcg ccggagtaat cgttttcagg     2100

tatataccgg atgttcattg ctttctaaat tttgctatgt tgccagtatc cttacgatgt     2160

atttatttta aggaaaagcc atatgacttc gatcttcgcc gaacagacgg ttgaggtggt     2220

aaaatcagcc atagaaaccg cggatggggc gctcgacctt tacaataagt accttgatca     2280

ggtgatcccg tggaaaacgt tcgacgagac tatcaaagaa ttatcacgat ttaagcagga     2340

atattcacag gaagcatccg tacttgttgg tgatattaaa gtcttactca tggattctca     2400

ggataagtac ttcgaggcaa cccagacggt gtacgagtgg tgtggcgttg taacacagct     2460

tctgtcggct tacattcttc tgttcgatga atataacgag aaaaaagcct ccgcccagaa     2520

agacattctg atacgcattc ttgacgatgg tgtgaagaag ctgaacgaag cacagaaatc     2580

gttattaact tcctctcagt cctttaataa cgcgtcaggc aagttactgg ctcttgattc     2640

ccagttgact aatgacttca gtgaaaaatc gtcgtatttc cagtcacaag ttgaccgtat     2700

ccgtaaagag gcttacgctg tcgctgctgc gggctcggtc agtggcccat tcggtctttc     2760

tatcagctat agcattgcag ccggagtcat agaaggcaaa ctgatcccgg agttgaacaa     2820

tcgcctgaaa accgtgcaaa atttttttac gagtttgagc gccactgtca aacaggcgaa     2880

caaggatata gatgctgcaa aactcaaatt agcgaccgaa attgccgcga taggtgaaat     2940

taagaccgaa acggagacaa cccggttcta cgtcgactac gacgacttga tgttatcatt     3000

gctgaaaggc gccgctaaaa agatgatcaa cacctgtaac gaatatcagc agcggcacgg     3060

aaaaaaaacc ctttttgagg tccctgatgt cgggcccaca tattactacg acgaagattc     3120

gaagttggtc aagggcctga taaacataaa caactcgtta ttttatttcg atcctattga     3180

atttaacctg gtgacggggt ggcagaccat aaacgggaag aagtactact ttgacatcaa     3240

taccggcgca gcattgattt catataagat aattaacggc aagcatttct actttaacaa     3300

cgatggagtc atgcaactgg gagtctttaa gggtcccgac ggcttcgaat actttgcccc     3360

agcgaacacc caaaacaaca atattgaggg gcaggcgatt gtctatcaat caaagttttt     3420

gacgctgaac ggtaagaaat actattttga taacgattcg aaagcagtca cggggtggcg     3480

gattattaac aacgaaaaat attattttaa tccaaataat gctatcgcag cagtcgggct     3540

tcaagtgatc gataataata agtactactt caatccagat acggctatta tttcaaaagg     3600

gtggcagact gtcaacggct ccaggtatta tttcgacact gatactgcta tcgctttcaa     3660

cgggtataag acaatcgatg gtaagcattt ctactttgat agcgactgcg tggttaaaat     3720

tggtgtattc agtacctcta atggatttga gtacttcgct cctgcaaaca cttacaataa     3780

caatattgaa ggtcaggcca tcgtatacca aagcaagttc ctcaccttaa atggcaaaaa     3840

gtactatttc gacaacaata gcaaagcggt caccggttgg cagaccattg atagtaaaaa     3900

atattatttt aataccaaca ctgcggaagc tgctaccgga tggcagacaa tcgacggcaa     3960

gaagtattat ttcaacacca atacagcaga agcggccaca gggtggcaaa cgatcgacgg     4020

gaagaagtac tactttaata ctaacacggc cattgctagc accggttata ccattattaa     4080

tgggaaacac ttttacttca acactgacgg cattatgcag atcggtgtat tcaaagggcc     4140

taacggcttc gaatatttcg caccggccaa tacagacgcg aacaatatag aaggacaggc     4200

gattctgtat cagaatgaat tcctgaccct gaatggtaag aaatattact tcggcagcga     4260

ttctaaggcc gtcaccgggt ggcggataat caataataaa aagtactatt tcaacccgaa     4320

taacgcgatt gcagctattc acctgtgcac gatcaacaat gataagtatt attttagcta     4380

tgatgggatc cttcaaaatg gatatattac aatagaaaga aataacttct atttcgatgc     4440

gaataatgag tctaaaatgg tgactggcgt tttcaaaggc ccaaatgggt tcgaatactt     4500

cgctccggcg aacacacaca acaacaatat tgaagggcag gcaatagtgt atcagaataa     4560

attcttgacg ctgaatggta aaaagtacta ctttgataat gattcgaaag cggtaacagg     4620

ctggcagacc atagacggca agaaatatta ctttaatctg aatactgccg aagctgcgac     4680

gggctggcaa accatagacg gaaagaaata ttattttaat ctgaacaccg cagaggccgc     4740

caccggatgg cagaccatcg acgggaagaa atactatttc aacactaata ccttcatagc     4800

gagtacgggg tatacctcga tcaatggcaa gcatttctac tttaacaccg acgggattat     4860

gcagatcggt gttttcaagg ggccgaacgg cttcgaatac ttcgctcccg caaacacaca     4920

caacaacaac atcgagggac aggctatact gtatcaaaat aaatttctta cgttaaatgg     4980

caagaagtat tattttgggt cggacagcaa agcagtgacc ggtttgcgta ccatagatgg     5040

taagaaatat tattttaata ctaacacggc agtagccgtt accggatggc agactattaa     5100

tgggaagaaa tactatttta acactaacac gagcattgcc tcgactggct acacgatcat     5160

tagcgggaaa cacttctact tcaacacgga tggtattatg cagataggtg tctttaaagg     5220

tcctgacggt tttgagtact tcgcacccgc caacaccgac gctaataaca tagaggggca     5280

agctatcagg tatcagaatc gcttccttta cctgcatgat aacatctatt acttcgggaa     5340

caacagtaag gctgctaccg ggtgggtgac aattgacggt aatcgctatt atttcgagcc     5400

taacacagca atgggagcca atggctataa gactatcgat aacaaaaatt tttactttcg     5460

gaacggtttg cctcaaatcg gggtttttaa aggatctaac ggcttcgagt actttgcccc     5520

ggcgaacacg gatgccaaca atattgaggg ccaggcgata aggtaccaga accgctttct     5580

gcatctcttg ggtaaaatct attacttcgg caacaactca aaggcggtaa caggatggca     5640

aactataaac gggaaggttt actattttat gcctgatacg gccatggctg cggcgggagg     5700

cctgttcgaa attgacggtg ttatatactt tttcggtgtg gacggtgtta aggccccagg     5760

catttacccg gctagactag cctaggtcca gcattaccgt gccgggacgt acgatcaacc     5820

ggatgggtga agaggtcgag atgatcacca aagggcgcca cgatccgtgt gtggggattc     5880

gcgcagtgcc gatcgcagaa gccatgctgg cgatcgtact gatggatcac ctgctgcgcc     5940

atcgggcaca gaatgcggat gtaaagacag agattccacg ctggtaagaa atgaaaaaaa     6000

ccgcgattgc gctgctggca tggtttgtca gtagcgccag cctggcggcg acgccgtggc     6060

agaaaataac ccatcctgtc cccggcgccg cccagtctat cggtagcttt gccaacggat     6120

gcatcattgg cgccgacacg ttgccggtac agtccgataa ttatcaggtg atgcgcaccg     6180

atcagcgccg ttatttcggc cacccggatc tggtcatgtt tatccagcgg ttgagtcatc     6240

aggcgcagca acgggggctc ggaaccgtcc tgataggcga catggggatg cctgccggag     6300

gccgctttaa tggcggacac gccagtcatc agaccgggct tgatgtggat attttcttgc     6360

agttgccgaa aacgcgctgg agccaggcgc agctattgcg cccgcaggcg ttagatctgg     6420

tgtcccgcga cggtaaacat gtcgtgccgt cgcgctggtc gtcggatatc gccagtctga     6480

tcaaactggc ggcacaagac aatgacgtca cccgtatttt cgtcaatccg gctattaaac     6540

aacagctttg cctcgatgcc ggaagcgatc gtgactggct acgtaaagta cgcccctggt     6600

tccagcatcg cgcgcatatg cacgtgcgtt tacgctgccc tgccgacagc ctggagtgcg     6660

aagatcaacc tttacccccg ccgggcgatg gatgcggcgc tgaactgcaa agctggttcg     6720

aaccgccaaa acctggcacc acaaagcctg agaagaagac accgccgccg ttgccgcctt     6780

cctgccaggc gctactggat gagcatgtac tctgatggac aatttttatg atctgtttat     6840

ggtctccccg ctgctgctgg tggtgctgtt ttttgtcgcc gtactggcag gatttatcga     6900

ttctatcgcc ggaggcggag ggctgctcac tatccctgcg ctgatggccg ccgggatgtc     6960

gccggcaaac gcgttggcga ccaataaatt acaggcgtgc ggcggctccc tctcgt         7016


<210>  23
<211>  1195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClyA-Toxin A repeat

<400>  23

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu 
            180                 185                 190         


Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Gly 
    290                 295                 300                 


Pro Thr Tyr Tyr Tyr Asp Glu Asp Ser Lys Leu Val Lys Gly Leu Ile 
305                 310                 315                 320 


Asn Ile Asn Asn Ser Leu Phe Tyr Phe Asp Pro Ile Glu Phe Asn Leu 
                325                 330                 335     


Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asp Ile 
            340                 345                 350         


Asn Thr Gly Ala Ala Leu Ile Ser Tyr Lys Ile Ile Asn Gly Lys His 
        355                 360                 365             


Phe Tyr Phe Asn Asn Asp Gly Val Met Gln Leu Gly Val Phe Lys Gly 
    370                 375                 380                 


Pro Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Gln Asn Asn Asn 
385                 390                 395                 400 


Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn 
                405                 410                 415     


Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp 
            420                 425                 430         


Arg Ile Ile Asn Asn Glu Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile 
        435                 440                 445             


Ala Ala Val Gly Leu Gln Val Ile Asp Asn Asn Lys Tyr Tyr Phe Asn 
    450                 455                 460                 


Pro Asp Thr Ala Ile Ile Ser Lys Gly Trp Gln Thr Val Asn Gly Ser 
465                 470                 475                 480 


Arg Tyr Tyr Phe Asp Thr Asp Thr Ala Ile Ala Phe Asn Gly Tyr Lys 
                485                 490                 495     


Thr Ile Asp Gly Lys His Phe Tyr Phe Asp Ser Asp Cys Val Val Lys 
            500                 505                 510         


Ile Gly Val Phe Ser Thr Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala 
        515                 520                 525             


Asn Thr Tyr Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser 
    530                 535                 540                 


Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asn Ser 
545                 550                 555                 560 


Lys Ala Val Thr Gly Trp Gln Thr Ile Asp Ser Lys Lys Tyr Tyr Phe 
                565                 570                 575     


Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly 
            580                 585                 590         


Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp 
        595                 600                 605             


Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Ile 
    610                 615                 620                 


Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe Tyr Phe Asn 
625                 630                 635                 640 


Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe 
                645                 650                 655     


Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln 
            660                 665                 670         


Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly Lys Lys Tyr 
        675                 680                 685             


Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg Ile Ile Asn 
    690                 695                 700                 


Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala Ala Ile His 
705                 710                 715                 720 


Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr Asp Gly Ile 
                725                 730                 735     


Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe Tyr Phe Asp 
            740                 745                 750         


Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys Gly Pro Asn 
        755                 760                 765             


Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn Asn Ile Glu 
    770                 775                 780                 


Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys 
785                 790                 795                 800 


Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly Trp Gln Thr 
                805                 810                 815     


Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala 
            820                 825                 830         


Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn 
        835                 840                 845             


Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr 
    850                 855                 860                 


Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr Thr Ser Ile 
865                 870                 875                 880 


Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly 
                885                 890                 895     


Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr 
            900                 905                 910         


His Asn Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Lys Phe 
        915                 920                 925             


Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala 
    930                 935                 940                 


Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr 
945                 950                 955                 960 


Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys 
                965                 970                 975     


Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly Tyr Thr Ile 
            980                 985                 990         


Ile Ser Gly Lys His Phe Tyr Phe  Asn Thr Asp Gly Ile  Met Gln Ile 
        995                 1000                 1005             


Gly Val  Phe Lys Gly Pro Asp  Gly Phe Glu Tyr Phe  Ala Pro Ala 
    1010                 1015                 1020             


Asn Thr  Asp Ala Asn Asn Ile  Glu Gly Gln Ala Ile  Arg Tyr Gln 
    1025                 1030                 1035             


Asn Arg  Phe Leu Tyr Leu His  Asp Asn Ile Tyr Tyr  Phe Gly Asn 
    1040                 1045                 1050             


Asn Ser  Lys Ala Ala Thr Gly  Trp Val Thr Ile Asp  Gly Asn Arg 
    1055                 1060                 1065             


Tyr Tyr  Phe Glu Pro Asn Thr  Ala Met Gly Ala Asn  Gly Tyr Lys 
    1070                 1075                 1080             


Thr Ile  Asp Asn Lys Asn Phe  Tyr Phe Arg Asn Gly  Leu Pro Gln 
    1085                 1090                 1095             


Ile Gly  Val Phe Lys Gly Ser  Asn Gly Phe Glu Tyr  Phe Ala Pro 
    1100                 1105                 1110             


Ala Asn  Thr Asp Ala Asn Asn  Ile Glu Gly Gln Ala  Ile Arg Tyr 
    1115                 1120                 1125             


Gln Asn  Arg Phe Leu His Leu  Leu Gly Lys Ile Tyr  Tyr Phe Gly 
    1130                 1135                 1140             


Asn Asn  Ser Lys Ala Val Thr  Gly Trp Gln Thr Ile  Asn Gly Lys 
    1145                 1150                 1155             


Val Tyr  Tyr Phe Met Pro Asp  Thr Ala Met Ala Ala  Ala Gly Gly 
    1160                 1165                 1170             


Leu Phe  Glu Ile Asp Gly Val  Ile Tyr Phe Phe Gly  Val Asp Gly 
    1175                 1180                 1185             


Val Lys  Ala Pro Gly Ile Tyr  
    1190                 1195 


<210>  24
<211>  6784
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClyA-Toxin B repeat fusion construct in ssaV and under the 
       control of an ssaG promoter

<400>  24
ctgaactttc ctcaacacga tatgccgttg agttttcact ttctcgtcat ttcaacgcgt       60

tactgaaatg gttacgtaat ggtgaagata aaagaggtag cgatgaatat taaaattaat      120

gagataaaaa tgacgccccc tacagcattt acccctggcc tggttataga ggaacaagag      180

gttatttcgc cttcaatgtt agctctccat gagttacagg aaacggcggg ggcagcgctc      240

tatgagacga tggaagaaat aggaatggcg ctgagtggta aactgcgcga aagtaataaa      300

ttcactgatg ctgagaaact ggagcgcagg cagcaggctt tgctgcgttt gataaaacaa      360

atacaggagg ataatggggc agcgttgcgt ccgcttaccg aagagaatag tgatcctgat      420

ttacagaatg cgtatcaaat tatcgctctt gcaatggcgc ttactgccgg cgggttgtca      480

aaaaagaaaa aacgcgattt gcaatcgcaa ctggatacgc ttacagcgga ggagggatgg      540

gaacttgccg tttttagttt actggaactt ggcgaagtgg ataccgctac gctgtcctcg      600

ctgaagcgtt ttatgcaaca ggcgatagac aacgatgaaa tgcccttatc gcagtggttc      660

agacgcgtgg cagactggcc ggatcgcggt gaacgggtcc gtattttgct aagagcaata      720

gcctttgaac ttagcatatg catcgaaccc tcggagcaaa gtcgtttggc cgcagcatta      780

gtacgcttgc gtcgtttgtt gttattcctt ggccttgaaa aagagtgcca gcgtgaggag      840

tggatttgcc agttgccgcc taatacatta ctgccgctac tactcgatat catttgtgag      900

cgctggcttt tcagcgattg gttgcttgat agacttaccg ctatagtttc ttcatcgaag      960

atgttcaatc ggttactcca acaacttgat gcgcagttta tgctgatacc cgataactgt     1020

tttaacgacg aagatcaacg tgaacaaatt ctcgaaacgc ttcgtgaagt aaagataaat     1080

caggttttat tctgatacct ggctttcaat atttaggtaa attggctttc tggctcatca     1140

tgaggcgtca ggatggattg ggatctcatt actgaacgta atattcagct ttttattcaa     1200

ttagcaggat tagctgaacg gcctttagca accaatatgt tctggcggca aggacaatat     1260

gaaacctgtc taaactatca taatggtcgt attcacttat gtcagatact caagcaaacc     1320

ttcttagacg aagaactgct ttttaaagcg ttggctaact ggaaacccgc agcgttccag     1380

ggtattcctc aacgattatt tttgttgcgc gatgggcttg caatgagttg ttctccacct     1440

ctttccagct ccgccgagct ctggttacga ttacatcatc gacaaataaa atttctggag     1500

tcgcaatgcg ttcatggtta ggtgagggaa tcagggcgca acagtggctc agtgtatgcg     1560

cgggtcgtca ggatatggtc ctagctcgag attgccatcg cggatgtcgc ctgtcttatc     1620

taccatcata aacatcattt gcctatggct cacgacagta taggcaatgc cgttttttat     1680

attgctaatt gtttcgccaa tcaacgcaaa agtatggcga ttgctaaagc cgtctccctg     1740

ggcggtagat tagccttaac cgcgacggta atgactcatt catactggag tggtagtttg     1800

ggactacagc ctcatttatt agagcgtctt aatgatatta cctatggact aatgagtttt     1860

actcgcttcg gtatggatgg gatggcaatg accggtatgc aggtcagcag cccattatat     1920

cgtttgctgg ctcaggtaac gccagaacaa cgtgcgccgg agtaatcgtt ttcaggtata     1980

taccggatgt tcattgcttt ctaaattttg ctatgttgcc agtatcctta cgatgtattt     2040

attttaagga aaagccatat gaccagcatt ttcgccgaac agactgtgga agtggtgaag     2100

tcggcaatcg aaaccgcgga cggcgctctg gatctgtata acaaatatct ggaccaggta     2160

atcccctgga aaaccttcga tgaaacgatc aaagaacttt cgaggtttaa gcaggaatat     2220

tcgcaggaag cctcagtcct cgtcggcgat atcaaagtgc tgctcatgga ttctcaggat     2280

aagtatttcg aagcaacgca gacggtctat gaatggtgtg gggtggtcac acagttactt     2340

tccgcataca tccttctgtt cgatgaatac aacgaaaaaa aggcatccgc gcagaaagat     2400

atcttaatca ggattcttga tgacggtgtt aagaaactga acgaagctca gaaatcgctg     2460

cttacaagct cccagtcgtt caacaatgcg tcaggtaaac tgttagcgct tgactcacag     2520

ttgacaaatg atttctctga aaagagcagt tatttccagt cccaggtgga tagaataaga     2580

aaagaggcat acgcggtggc agccgctggt tcggtgtccg ggccattcgg tctgtcgatt     2640

tcttatagca ttgcggctgg tgttatcgag ggaaagctga ttccggagct taataaccga     2700

cttaagaccg tgcagaactt ctttacttca ctcagcgcga cagtcaagca ggccaacaag     2760

gatatcgacg ccgccaaact caagctggcc acagaaattg ctgcaatcgg tgagataaag     2820

acagagacag aaacgacccg cttctatgtg gactatgatg accttatgtt gagtctcctt     2880

aaaggagccg ccaaaaagat gataaacacg tgcaacgagt atcaacaaag gcatggaaaa     2940

aagacattat ttgaagttcc agacgttccc gggaagtttt atatcaacaa cttcggcatg     3000

atggtgtctg gcttgatcta catcaacgat agcctctatt atttcaagcc gcccgttaat     3060

aacttaatca caggcttcgt gacagtaggt gatgacaaat actattttaa tccgatcaat     3120

ggaggcgcag caagtattgg tgaaacgata atcgacgaca agaactatta ttttaaccaa     3180

tcaggagtgc tgcaaactgg tgtgttttcc accgaggacg gctttaagta cttcgccccc     3240

gcgaacaccc tggacgaaaa ccttgagggt gaagccattg acttcactgg taaacttatt     3300

atcgacgaaa acatctacta ttttgatgat aactacagag gcgcagtgga gtggaaagag     3360

ctggacgggg aaatgcatta cttttcccca gagacaggta aagctttcaa aggtctgaat     3420

cagattgggg attacaaata ttacttcaac tctgacggtg tcatgcagaa gggatttgtg     3480

tcaatcaacg ataataagca ctactttgat gactcaggag taatgaaggt gggctacacg     3540

gagattgacg gaaaacattt ctatttcgcc gaaaatggtg aaatgcagat tggcgttttc     3600

aataccgagg atggcttcaa gtattttgct catcacaatg aggatctggg aaacgaagaa     3660

ggcgaggaaa tttcctactc gggcatactg aattttaaca ataaaatata ttatttcgac     3720

gacagtttta cggcggttgt tgggtggaag gatttagaag atggtagtaa atactacttc     3780

gatgaggaca cggccgaagc ctatatcggt ttgtcgctga ttaatgatgg acagtactat     3840

tttaatgacg acggcattat gcaagttggg ttcgtgacca ttaacgacaa agtgttttat     3900

ttttcagact caggaattat cgagagcggg gttcaaaaca ttgatgataa ttatttttac     3960

atagacgata atgggatcgt tcagatcggg gtgttcgaca catctgacgg ttacaaatat     4020

tttgctcccg caaatacggt gaacgacaac atttacgggc aggcagtgga atattcgggt     4080

ttggttagag ttggcgagga tgtctactat tttggcgaga catacacgat tgaaacgggg     4140

tggatttacg atatggagaa cgaaagcgat aaatattact ttaacccaga aacaaagaag     4200

gcctgcaaag gtatcaattt aatcgatgat atcaaatact atttcgacga aaagggtatc     4260

atgcgtactg ggctgatcag ctttgagaac aataattact atttcaatga aaatggggaa     4320

atgcaatttg gatatattaa tatagaagat aagatgtttt atttcgggga ggatggtgtg     4380

atgcagatcg gcgttttcaa caccccggac gggtttaaat atttcgcaca tcagaataca     4440

ctggatgaga acttcgaggg tgagtctatt aactacaccg ggtggctgga cttagacgag     4500

aaacgctact atttcacaga cgagtacatt gcagctactg gttcggtcat cattgatggc     4560

gaggaatatt atttcgaccc ggataccgcc cagttagtga tctccgagta atctagacta     4620

gcctaggcta gtctagactt atacaagtgg tagaaagtat tgaccttagc gaagaggagt     4680

tggcggacaa tgaagaatga attgatgcaa cgtctgaggc tgaaatatcc gccccccgat     4740

ggttattgtc gatggggccg aattcaagat gtcagcgcaa cgttgttaaa tgcgtggttg     4800

cctggggtat ttatgggaga gttgtgctgt ataaagcctg gagaagaact tgctgaagtc     4860

gtggggatta atggcagcaa agctttgcta tctcctttta cgagtactat cgggcttcac     4920

tgcgggcagc aagtgatggc cttaaggcga cgccatcagg ttcccgtggg cgaagcgtta     4980

ttagggcgag tcattgatgg ttttggtcgt ccccttgatg gctgcgaact gcccgacgtc     5040

tgctggaaag actatgatgc aatgcctcct cccgcaatgg ttcgacagcc tatcactcaa     5100

ccattaatga cggggattcg cgctattgat agcgttgcga cctgtggtga agggcaacga     5160

gtgggtattt tttctgctcc tggcgtgggg aaaagcacgc ttctggcgat gctgtgtaat     5220

gcgccagacg cagactgcaa tgttctggtg ttaattggtg aacgtggacg agaagtccgc     5280

gagttcatcg attttacact gtctgaagag acccgaaaac gttgtgtcat tgttgtcgca     5340

acctctgaca gacccgcctt agagcgcgtg agggcgctgt ttgtggccac cacgatagca     5400

gaattttttc gcgataatgg aaaacgagtc gtcttgcttg ccgactcact gacgcgttat     5460

gccagggccg cacgggaaat cgctctggcc gccggagaga ccgcagtttc tggagaatat     5520

ccgccaggcg tatttagtgc attgccacga cttttagaac gtacaggaat gggggaaaaa     5580

ggcagtatta ccgcatttta tacggtcctg gtggaaggcg atgatatgaa tgagccgttg     5640

gcggatgaag tccgttcact gcttgacgga catattgtgc tatcccggcg gcttgcagag     5700

agggggcatt atcctgccat tgacgtgttg gcaacgctca gccgcgtttt tccagtcgtt     5760

accagccatg agcatcgtca actggcggct atattgcgac ggcgcctggc gctttaccag     5820

gaggttgaac tgttaatacg cattggggaa taccagcgag gagttgatac tgataccgat     5880

aaagccattg atacctatcc ggatatttgc acatttttgc gacaaagtaa ggatgaagta     5940

tgcggacccg agctactcat agaaaaatta catcaaatac tcaccgagtg atcatggaaa     6000

ctttgctgga gataatcgcg cggcgtgaaa agcaattacg cagcaaactt accgtgcttg     6060

atcagcagca acaggcgatt attactgaac agcagatttg ccagacgcgc gctttagcag     6120

tgactaccag actgaaagaa ttaatgggct ggcaaggtac gttatcttgt catttattgt     6180

tggataagaa acaacaaatg gccggactat tcactcaggc gcagagcttt ttgacgcaac     6240

ggcagcagtt agagaatcag tatcagcagc ttgtctccag gcgaagcgaa ttacagaaga     6300

attttaatgc gcttatgaaa aagaaagaaa aaattactat ggtattaagc gatgcgtatt     6360

accaaagttg agggaagtct tgggttgcca tgccagtctt atcaggatga taacgaggcg     6420

gaggcggaac gtatggactt tgaacaactc atgcaccagg cattacccat tggtgagaat     6480

aatcctcctg cagcattgaa taagaacgtg gttttcacgc aacgttatcg tgttagtggc     6540

ggttatcttg acggtgtaga gtgtgaagtc tgtgagtcag gagggctaat ccagttaaga     6600

atcaatgtcc ctcatcatga aatttaccgt tcgatgaaag cgctaaagca gtggctggag     6660

tctcagttgc tgcatatggg gtatataatt tccctggaga tattctatgt taagaatagc     6720

gaatgaagag cgtccgtggg tggagatact tccaacacaa ggcgctacca ttggtgagct     6780

gaca                                                                  6784


<210>  25
<211>  850
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClyA-Toxin B repeat fusion

<400>  25

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Val Ala Ala Ala Gly Ser Val Ser Gly Pro Phe Gly Leu 
            180                 185                 190         


Ser Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Pro 
    290                 295                 300                 


Gly Lys Phe Tyr Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Ile 
305                 310                 315                 320 


Tyr Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn Leu 
                325                 330                 335     


Ile Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro 
            340                 345                 350         


Ile Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys 
        355                 360                 365             


Asn Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser 
    370                 375                 380                 


Thr Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu 
385                 390                 395                 400 


Asn Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp 
                405                 410                 415     


Glu Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp 
            420                 425                 430         


Lys Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys 
        435                 440                 445             


Ala Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn 
    450                 455                 460                 


Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys 
465                 470                 475                 480 


His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile 
                485                 490                 495     


Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly 
            500                 505                 510         


Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu 
        515                 520                 525             


Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu 
    530                 535                 540                 


Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val 
545                 550                 555                 560 


Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu 
                565                 570                 575     


Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln 
            580                 585                 590         


Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile 
        595                 600                 605             


Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly 
    610                 615                 620                 


Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile 
625                 630                 635                 640 


Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala 
                645                 650                 655     


Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr 
            660                 665                 670         


Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr 
        675                 680                 685             


Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp 
    690                 695                 700                 


Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn 
705                 710                 715                 720 


Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg 
                725                 730                 735     


Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn 
            740                 745                 750         


Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr 
        755                 760                 765             


Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp 
    770                 775                 780                 


Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu 
785                 790                 795                 800 


Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg 
                805                 810                 815     


Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile 
            820                 825                 830         


Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile 
        835                 840                 845             


Ser Glu 
    850 


<210>  26
<211>  66
<212>  DNA
<213>  Escherichia coli

<400>  26
atgttaaaaa taaaatactt attaataggt ctttcactgt cagctatgag ttcatactca       60

ctagct                                                                  66


<210>  27
<211>  22
<212>  PRT
<213>  Escherichia coli

<400>  27

Met Leu Lys Ile Lys Tyr Leu Leu Ile Gly Leu Ser Leu Ser Ala Met 
1               5                   10                  15      


Ser Ser Tyr Ser Leu Ala 
            20          


