                         SEQUENCE LISTING

<110>  Sapphire Energy, Inc.
       Mendez, Michael
       O'Neill, Bryan
       Burkart, Michael
       Lieberman, Soyan
       Bielinski, Vince
       Behnke, Craig
       Poon, Yan
 
<120>  PRODUCTION OF FATTY ACIDS BY GENETICALLY MODIFIED PHOTOSYNTHETIC 
       ORGANISMS

<130>  0713WO1

<150>  US 61/088,591
<151>  2008-08-13

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  1
gtgctaggta actaacgttt gattttt                                           27


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  2
ccgaactgag gttgggttta                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
gggggagcga ataggattag                                                   20


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
ggaaggggac gtaggtacat aaa                                               23


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
ttagaacgtg ttttgttccc aat                                               23


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
tggtacaaga ggatttttgt tgtt                                              24


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
tggtacaaga ggatttttgt tgtt                                              24


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
ccccttacgg gcaagtaaac                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
tgtttgttaa ggctagctgc                                                   20


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
cgccactgtc atcctttaag t                                                 21


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
attccaccat ctgctaatgc tt                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
tgtaccccat gtaaatgtcc aa                                                22


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
cctgacatta cacgctcagg t                                                 21


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
attccaccat ctgctaatgc tt                                                22


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
attccaccat ctgctaatgc tt                                                22


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
cctgacatta cacgctcagg t                                                 21


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
cattccaacg aggagttaaa c                                                 21


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
actcccgccg gtactcaatg gtg                                               23


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
attccaccat ctgctaatgc tt                                                22


<210>  20
<211>  1275
<212>  DNA
<213>  Chlamydomonas reinhardtii


<220>
<221>  misc_feature
<223>  Full length thioesterase including N-terminal chloroplast 
       targeting signal

<220>
<221>  misc_feature
<222>  (1192)..(1272)
<223>  FLAG

<400>  20
atggtaccaa gacgttttgc aacattaaat gaacaagctc aaagtactgc ttctacatca       60

agtagtactc gtagtgtttg tgctcgtatt tgttctaatc gtagacgtgc ttcaattcag      120

ccattaggta cagtagcttt aggtcgtcgt tcaggtgttg catgcagaag tggtgtagca      180

gttcaggcag ctgctgttgt acaagaagaa tacgatgtag ttattcctaa atttcctgct      240

ccagctgcag gtgcattctt acctgataaa cgttcattta gagaagagca tagaattaga      300

ggatatgagg tatctccaga ccaacgtgct acaattgtta cagttgctaa cttattacaa      360

gaagtagctg gaaatcatgc tgtaggcatg tggggtcgta ctgatgaagg tttcgctagt      420

ttaccatcta tgaaagactt attattcgta atgactcgtc ttcaagttcg tatgtatgaa      480

tacccaaaat ggggtgacgt tgttgcagta gaaacttatt tcactgaaga aggtcgttta      540

gcttttcgtc gtgagtggaa attaatggac gtagctacag gtaaattatt aggcgctggt      600

acttcaactt gggtaacaat aaatacagca actcgtcgtt tatcaaaatt accagaagat      660

gttcgtaaac gtttcttacg ttttgctcct ccttcatctg tacatatttt acctccagaa      720

gaaactaaaa aaaaacttca agatatggaa ttaccaggac aagttcaatc tgctcaacaa      780

gttgctcgtc gtgctgatat ggacatgaac ggccacatta acaatgttac atatcttgct      840

tggacattag aatcattacc tgagcgtgta atgtcaggtg gctataaaat gcaagaaatt      900

gaattagatt tcaaagctga gtgcactgca ggtaacgcta ttgaggcaca ttgtaaccct      960

ttagatgatc atagtgcttc atttgttggt ccagcaccag ctaatggcaa tggtaatggt     1020

cacgctgcag aaccagcagc tgattcagct ccactttact ttctttctat gcttcaaaaa     1080

tgtgatgaaa atggttgcac agaattagta cgtgctcgta caacttggag tcgtacactt     1140

gaaggtgcaa aacctgcacc accaccatta tctgaattat cagcagctca aggtaccggt     1200

gaaaatttat attttcaagg ttcaggaggt ggtggctctg attataaaga tgatgatgat     1260

aaaggaaccg gttaa                                                      1275


<210>  21
<211>  424
<212>  PRT
<213>  Chlamydomonas reinhardtii


<220>
<221>  MISC_FEATURE
<223>  Full length thioesterase including N-terminal chloroplast 
       targeting signal

<220>
<221>  MISC_FEATURE
<222>  (398)..(424)
<223>  FLAG

<400>  21

Met Val Pro Arg Arg Phe Ala Thr Leu Asn Glu Gln Ala Gln Ser Thr 
1               5                   10                  15      


Ala Ser Thr Ser Ser Ser Thr Arg Ser Val Cys Ala Arg Ile Cys Ser 
            20                  25                  30          


Asn Arg Arg Arg Ala Ser Ile Gln Pro Leu Gly Thr Val Ala Leu Gly 
        35                  40                  45              


Arg Arg Ser Gly Val Ala Cys Arg Ser Gly Val Ala Val Gln Ala Ala 
    50                  55                  60                  


Ala Val Val Gln Glu Glu Tyr Asp Val Val Ile Pro Lys Phe Pro Ala 
65                  70                  75                  80  


Pro Ala Ala Gly Ala Phe Leu Pro Asp Lys Arg Ser Phe Arg Glu Glu 
                85                  90                  95      


His Arg Ile Arg Gly Tyr Glu Val Ser Pro Asp Gln Arg Ala Thr Ile 
            100                 105                 110         


Val Thr Val Ala Asn Leu Leu Gln Glu Val Ala Gly Asn His Ala Val 
        115                 120                 125             


Gly Met Trp Gly Arg Thr Asp Glu Gly Phe Ala Ser Leu Pro Ser Met 
    130                 135                 140                 


Lys Asp Leu Leu Phe Val Met Thr Arg Leu Gln Val Arg Met Tyr Glu 
145                 150                 155                 160 


Tyr Pro Lys Trp Gly Asp Val Val Ala Val Glu Thr Tyr Phe Thr Glu 
                165                 170                 175     


Glu Gly Arg Leu Ala Phe Arg Arg Glu Trp Lys Leu Met Asp Val Ala 
            180                 185                 190         


Thr Gly Lys Leu Leu Gly Ala Gly Thr Ser Thr Trp Val Thr Ile Asn 
        195                 200                 205             


Thr Ala Thr Arg Arg Leu Ser Lys Leu Pro Glu Asp Val Arg Lys Arg 
    210                 215                 220                 


Phe Leu Arg Phe Ala Pro Pro Ser Ser Val His Ile Leu Pro Pro Glu 
225                 230                 235                 240 


Glu Thr Lys Lys Lys Leu Gln Asp Met Glu Leu Pro Gly Gln Val Gln 
                245                 250                 255     


Ser Ala Gln Gln Val Ala Arg Arg Ala Asp Met Asp Met Asn Gly His 
            260                 265                 270         


Ile Asn Asn Val Thr Tyr Leu Ala Trp Thr Leu Glu Ser Leu Pro Glu 
        275                 280                 285             


Arg Val Met Ser Gly Gly Tyr Lys Met Gln Glu Ile Glu Leu Asp Phe 
    290                 295                 300                 


Lys Ala Glu Cys Thr Ala Gly Asn Ala Ile Glu Ala His Cys Asn Pro 
305                 310                 315                 320 


Leu Asp Asp His Ser Ala Ser Phe Val Gly Pro Ala Pro Ala Asn Gly 
                325                 330                 335     


Asn Gly Asn Gly His Ala Ala Glu Pro Ala Ala Asp Ser Ala Pro Leu 
            340                 345                 350         


Tyr Phe Leu Ser Met Leu Gln Lys Cys Asp Glu Asn Gly Cys Thr Glu 
        355                 360                 365             


Leu Val Arg Ala Arg Thr Thr Trp Ser Arg Thr Leu Glu Gly Ala Lys 
    370                 375                 380                 


Pro Ala Pro Pro Pro Leu Ser Glu Leu Ser Ala Ala Gln Gly Thr Gly 
385                 390                 395                 400 


Glu Asn Leu Tyr Phe Gln Gly Ser Gly Gly Gly Gly Ser Asp Tyr Lys 
                405                 410                 415     


Asp Asp Asp Asp Lys Gly Thr Gly 
            420                 


<210>  22
<211>  1824
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  misc_feature
<223>  Full length acyl-CoA synthetase including N-terminal chloroplast 
       targeting signal

<220>
<221>  misc_feature
<222>  (581)..(607)
<223>  FLAG

<400>  22
atggtaccag acaaccttgc tttatgtgaa gctaataacg ttccattaac accaattact       60

ttcttaaaac gtgcatctga atgctatcca aacagaacat caattatcta cggtaaaact      120

cgttttacat ggccacaaac ttatgaccgt tgctgtcgtt tagctgctag tttaatcagt      180

ttaaacattg gtaaaaacga tgttgtttca gtagtagctc ctaatacacc tgctatgtac      240

gagatgcact ttgctgttcc tatggctggt gctgtattaa atccaattaa tacacgttta      300

gatgctacat ctattgcagc tattttacgt cacgctaaac ctaaaattct ttttattgat      360

cgttcatttg agcctcttgc tcgtgagatt ttacaacttt tatcatcaga agattcaaac      420

ttaaacttac cagtaatctt tatccatgaa attgatttcc caaaacgtgt ttcatctgag      480

gaatcagatt atgaatgttt aattcaacgt ggtgaaccta cacctagttt attagcaaga      540

atgttctgta tacaagacga acatgaccct attagtttaa actatacatc aggaactact      600

gcagatccaa aaggtgttgt aatttcacat cgtggtgctt atttatcaac attatctgct      660

attatcggtt gggaaatggg tacatgtcca gtatacttat ggacattacc tatgtttcat      720

tgcaacggtt ggacatttac atggggtaca gcagctcgtg gtggtacatc agtatgtatg      780

cgtcacgtta cagcacctga aatttacaaa aacattgaga tgcacaatgt aactcacatg      840

tgttgcgttc ctactgtatt taacatctta cttaaaggaa actctttaga tttaagtcac      900

cgttctggtc ctgtacacgt attaacaggt ggtagtccac ctccagcagc tttagttaaa      960

aaagttcaac gtcttggctt ccaagtaatg catgcttacg gattaacaga agctactggt     1020

ccagttttat tctgtgagtg gcaagatgaa tggaatcgtt taccagaaaa tcaacaaatg     1080

gaattaaaag ctcgtcaagg tctttcaatc ttaggtttaa ctgaagttga tgttcgtaat     1140

aaagaaactc aagaatcagt tcctagagac ggtaaaacaa tgggtgagat agttatgaaa     1200

ggatcttcaa tcatgaaagg ttacttaaaa aacccaaaag ctacttatga agcatttaaa     1260

cacggttggt taaattctgg tgatgtaggt gttattcacc cagatggtca tgttgaaatt     1320

aaagaccgta gtaaagacat cattattagt ggaggtgaaa acataagttc tgtagaagtt     1380

gaaaacatca tttacaaata cccaaaagtt ttagagacag cagtagttgc aatgccacac     1440

ccaacatggg gtgaaacacc atgtgctttc gtagttttag aaaaaggcga aacaaataat     1500

gaagaccgtg aagataaatt agttacaaaa gaacgtgact taattgaata ttgtcgtgag     1560

aatttaccac actttatgtg tccacgtaaa gtagtattct tagacgaatt accaaaaaat     1620

ggtaacggaa aaatacttaa accaaaatta cgtgacattg caaaaggttt agtagctgaa     1680

gatgaagtaa atgttcgttc taaagtacaa cgtcctgttg agcactttac ttctcgtctt     1740

ggtaccggtg aaaacttata ctttcaagga tcaggtggtg gtggtagtga ttataaagat     1800

gatgacgaca aaggaaccgg ttaa                                            1824


<210>  23
<211>  607
<212>  PRT
<213>  Arabidopsis thaliana


<220>
<221>  MISC_FEATURE
<223>  Full length acyl-CoA synthetase including N-terminal chloroplast 
       targeting signal

<220>
<221>  MISC_FEATURE
<222>  (581)..(607)
<223>  FLAG

<400>  23

Met Val Pro Asp Asn Leu Ala Leu Cys Glu Ala Asn Asn Val Pro Leu 
1               5                   10                  15      


Thr Pro Ile Thr Phe Leu Lys Arg Ala Ser Glu Cys Tyr Pro Asn Arg 
            20                  25                  30          


Thr Ser Ile Ile Tyr Gly Lys Thr Arg Phe Thr Trp Pro Gln Thr Tyr 
        35                  40                  45              


Asp Arg Cys Cys Arg Leu Ala Ala Ser Leu Ile Ser Leu Asn Ile Gly 
    50                  55                  60                  


Lys Asn Asp Val Val Ser Val Val Ala Pro Asn Thr Pro Ala Met Tyr 
65                  70                  75                  80  


Glu Met His Phe Ala Val Pro Met Ala Gly Ala Val Leu Asn Pro Ile 
                85                  90                  95      


Asn Thr Arg Leu Asp Ala Thr Ser Ile Ala Ala Ile Leu Arg His Ala 
            100                 105                 110         


Lys Pro Lys Ile Leu Phe Ile Asp Arg Ser Phe Glu Pro Leu Ala Arg 
        115                 120                 125             


Glu Ile Leu Gln Leu Leu Ser Ser Glu Asp Ser Asn Leu Asn Leu Pro 
    130                 135                 140                 


Val Ile Phe Ile His Glu Ile Asp Phe Pro Lys Arg Val Ser Ser Glu 
145                 150                 155                 160 


Glu Ser Asp Tyr Glu Cys Leu Ile Gln Arg Gly Glu Pro Thr Pro Ser 
                165                 170                 175     


Leu Leu Ala Arg Met Phe Cys Ile Gln Asp Glu His Asp Pro Ile Ser 
            180                 185                 190         


Leu Asn Tyr Thr Ser Gly Thr Thr Ala Asp Pro Lys Gly Val Val Ile 
        195                 200                 205             


Ser His Arg Gly Ala Tyr Leu Ser Thr Leu Ser Ala Ile Ile Gly Trp 
    210                 215                 220                 


Glu Met Gly Thr Cys Pro Val Tyr Leu Trp Thr Leu Pro Met Phe His 
225                 230                 235                 240 


Cys Asn Gly Trp Thr Phe Thr Trp Gly Thr Ala Ala Arg Gly Gly Thr 
                245                 250                 255     


Ser Val Cys Met Arg His Val Thr Ala Pro Glu Ile Tyr Lys Asn Ile 
            260                 265                 270         


Glu Met His Asn Val Thr His Met Cys Cys Val Pro Thr Val Phe Asn 
        275                 280                 285             


Ile Leu Leu Lys Gly Asn Ser Leu Asp Leu Ser His Arg Ser Gly Pro 
    290                 295                 300                 


Val His Val Leu Thr Gly Gly Ser Pro Pro Pro Ala Ala Leu Val Lys 
305                 310                 315                 320 


Lys Val Gln Arg Leu Gly Phe Gln Val Met His Ala Tyr Gly Leu Thr 
                325                 330                 335     


Glu Ala Thr Gly Pro Val Leu Phe Cys Glu Trp Gln Asp Glu Trp Asn 
            340                 345                 350         


Arg Leu Pro Glu Asn Gln Gln Met Glu Leu Lys Ala Arg Gln Gly Leu 
        355                 360                 365             


Ser Ile Leu Gly Leu Thr Glu Val Asp Val Arg Asn Lys Glu Thr Gln 
    370                 375                 380                 


Glu Ser Val Pro Arg Asp Gly Lys Thr Met Gly Glu Ile Val Met Lys 
385                 390                 395                 400 


Gly Ser Ser Ile Met Lys Gly Tyr Leu Lys Asn Pro Lys Ala Thr Tyr 
                405                 410                 415     


Glu Ala Phe Lys His Gly Trp Leu Asn Ser Gly Asp Val Gly Val Ile 
            420                 425                 430         


His Pro Asp Gly His Val Glu Ile Lys Asp Arg Ser Lys Asp Ile Ile 
        435                 440                 445             


Ile Ser Gly Gly Glu Asn Ile Ser Ser Val Glu Val Glu Asn Ile Ile 
    450                 455                 460                 


Tyr Lys Tyr Pro Lys Val Leu Glu Thr Ala Val Val Ala Met Pro His 
465                 470                 475                 480 


Pro Thr Trp Gly Glu Thr Pro Cys Ala Phe Val Val Leu Glu Lys Gly 
                485                 490                 495     


Glu Thr Asn Asn Glu Asp Arg Glu Asp Lys Leu Val Thr Lys Glu Arg 
            500                 505                 510         


Asp Leu Ile Glu Tyr Cys Arg Glu Asn Leu Pro His Phe Met Cys Pro 
        515                 520                 525             


Arg Lys Val Val Phe Leu Asp Glu Leu Pro Lys Asn Gly Asn Gly Lys 
    530                 535                 540                 


Ile Leu Lys Pro Lys Leu Arg Asp Ile Ala Lys Gly Leu Val Ala Glu 
545                 550                 555                 560 


Asp Glu Val Asn Val Arg Ser Lys Val Gln Arg Pro Val Glu His Phe 
                565                 570                 575     


Thr Ser Arg Leu Gly Thr Gly Glu Asn Leu Tyr Phe Gln Gly Ser Gly 
            580                 585                 590         


Gly Gly Gly Ser Asp Tyr Lys Asp Asp Asp Asp Lys Gly Thr Gly 
        595                 600                 605         


<210>  24
<211>  882
<212>  DNA
<213>  Lycopersicon hirsutum


<220>
<221>  misc_feature
<223>  Full length methylketone synthase including N-terminal 
       chloroplast targeting signal

<220>
<221>  misc_feature
<222>  (798)..(879)
<223>  FLAG

<400>  24
atggtaccag aaaaatctat gtcacccttt gtaaagaaac attttgtttt agtacatact       60

gctttccacg gtgcttggtg ttggtacaaa attgttgcct taatgcgctc aagcggtcat      120

aatgttaccg cccttgattt agcttcaggt attaacccaa aacaagctct tcaaattcct      180

aacttctcag actatttaag tcctttaatg gaatttatgg ctagtttacc agccaatgag      240

aaaatcattt tagtaggtca tgccttaggc ggtttagcaa tctcaaaagc aatggaaaca      300

tttccagaaa agattagtgt agcagtattc ttatcaggtc ttatgccagg acctaacatt      360

gatgccacaa ctgtatgcac aaaagctggt tcagcagtat taggccagtt agataattgc      420

gttacatacg aaaatggtcc aacaaaccca cctacaacat taattgctgg tcctaaattc      480

ttagcaacta atgtttacca tttatcacca atcgaagatt tagccttagc aacagcttta      540

gtacgtccat tatacttata tcttgctgaa gatatttcaa aagaagttgt tttaagctca      600

aaacgttatg gctcagttaa acgtgtattt atagtagcta cagaaaacga tgctcttaaa      660

aaggagttct taaaattaat gatcgaaaag aatcctcctg atgaagtaaa agaaattgaa      720

ggtagtgatc atgttacaat gatgagcaaa ccacaacaat tattcactac tttattatct      780

attgctaaca aatacaaagg taccggtgaa aatctttatt ttcaaggtag cggtggtggt      840

ggttcagatt ataaagacga tgatgataaa ggaaccggat aa                         882


<210>  25
<211>  293
<212>  PRT
<213>  Lycopersicon hirsutum


<220>
<221>  MISC_FEATURE
<223>  Full length methylketone synthase including N-terminal 
       chloroplast targeting signal

<220>
<221>  MISC_FEATURE
<222>  (267)..(293)
<223>  FLAG

<400>  25

Met Val Pro Glu Lys Ser Met Ser Pro Phe Val Lys Lys His Phe Val 
1               5                   10                  15      


Leu Val His Thr Ala Phe His Gly Ala Trp Cys Trp Tyr Lys Ile Val 
            20                  25                  30          


Ala Leu Met Arg Ser Ser Gly His Asn Val Thr Ala Leu Asp Leu Ala 
        35                  40                  45              


Ser Gly Ile Asn Pro Lys Gln Ala Leu Gln Ile Pro Asn Phe Ser Asp 
    50                  55                  60                  


Tyr Leu Ser Pro Leu Met Glu Phe Met Ala Ser Leu Pro Ala Asn Glu 
65                  70                  75                  80  


Lys Ile Ile Leu Val Gly His Ala Leu Gly Gly Leu Ala Ile Ser Lys 
                85                  90                  95      


Ala Met Glu Thr Phe Pro Glu Lys Ile Ser Val Ala Val Phe Leu Ser 
            100                 105                 110         


Gly Leu Met Pro Gly Pro Asn Ile Asp Ala Thr Thr Val Cys Thr Lys 
        115                 120                 125             


Ala Gly Ser Ala Val Leu Gly Gln Leu Asp Asn Cys Val Thr Tyr Glu 
    130                 135                 140                 


Asn Gly Pro Thr Asn Pro Pro Thr Thr Leu Ile Ala Gly Pro Lys Phe 
145                 150                 155                 160 


Leu Ala Thr Asn Val Tyr His Leu Ser Pro Ile Glu Asp Leu Ala Leu 
                165                 170                 175     


Ala Thr Ala Leu Val Arg Pro Leu Tyr Leu Tyr Leu Ala Glu Asp Ile 
            180                 185                 190         


Ser Lys Glu Val Val Leu Ser Ser Lys Arg Tyr Gly Ser Val Lys Arg 
        195                 200                 205             


Val Phe Ile Val Ala Thr Glu Asn Asp Ala Leu Lys Lys Glu Phe Leu 
    210                 215                 220                 


Lys Leu Met Ile Glu Lys Asn Pro Pro Asp Glu Val Lys Glu Ile Glu 
225                 230                 235                 240 


Gly Ser Asp His Val Thr Met Met Ser Lys Pro Gln Gln Leu Phe Thr 
                245                 250                 255     


Thr Leu Leu Ser Ile Ala Asn Lys Tyr Lys Gly Thr Gly Glu Asn Leu 
            260                 265                 270         


Tyr Phe Gln Gly Ser Gly Gly Gly Gly Ser Asp Tyr Lys Asp Asp Asp 
        275                 280                 285             


Asp Lys Gly Thr Gly 
    290             


<210>  26
<211>  1023
<212>  DNA
<213>  Chlamydomonas reinhardtii


<220>
<221>  misc_feature
<223>  Thioesterase N-terminal truncation

<220>
<221>  misc_feature
<222>  (940)..(1020)
<223>  FLAG

<400>  26
atggtaccac ctgataaacg ttcatttaga gaagagcata gaattagagg atatgaggta       60

tctccagacc aacgtgctac aattgttaca gttgctaact tattacaaga agtagctgga      120

aatcatgctg taggcatgtg gggtcgtact gatgaaggtt tcgctagttt accatctatg      180

aaagacttat tattcgtaat gactcgtctt caagttcgta tgtatgaata cccaaaatgg      240

ggtgacgttg ttgcagtaga aacttatttc actgaagaag gtcgtttagc ttttcgtcgt      300

gagtggaaat taatggacgt agctacaggt aaattattag gcgctggtac ttcaacttgg      360

gtaacaataa atacagcaac tcgtcgttta tcaaaattac cagaagatgt tcgtaaacgt      420

ttcttacgtt ttgctcctcc ttcatctgta catattttac ctccagaaga aactaaaaaa      480

aaacttcaag atatggaatt accaggacaa gttcaatctg ctcaacaagt tgctcgtcgt      540

gctgatatgg acatgaacgg ccacattaac aatgttacat atcttgcttg gacattagaa      600

tcattacctg agcgtgtaat gtcaggtggc tataaaatgc aagaaattga attagatttc      660

aaagctgagt gcactgcagg taacgctatt gaggcacatt gtaacccttt agatgatcat      720

agtgcttcat ttgttggtcc agcaccagct aatggcaatg gtaatggtca cgctgcagaa      780

ccagcagctg attcagctcc actttacttt ctttctatgc ttcaaaaatg tgatgaaaat      840

ggttgcacag aattagtacg tgctcgtaca acttggagtc gtacacttga aggtgcaaaa      900

cctgcaccac caccattatc tgaattatca gcagctcaag gtaccggtga aaatttatat      960

tttcaaggtt caggaggtgg tggctctgat tataaagatg atgatgataa aggaaccggt     1020

taa                                                                   1023


<210>  27
<211>  340
<212>  PRT
<213>  Chlamydomonas reinhardtii


<220>
<221>  MISC_FEATURE
<223>  Thioesterase N-terminal truncation

<220>
<221>  MISC_FEATURE
<222>  (314)..(340)
<223>  FLAG

<400>  27

Met Val Pro Pro Asp Lys Arg Ser Phe Arg Glu Glu His Arg Ile Arg 
1               5                   10                  15      


Gly Tyr Glu Val Ser Pro Asp Gln Arg Ala Thr Ile Val Thr Val Ala 
            20                  25                  30          


Asn Leu Leu Gln Glu Val Ala Gly Asn His Ala Val Gly Met Trp Gly 
        35                  40                  45              


Arg Thr Asp Glu Gly Phe Ala Ser Leu Pro Ser Met Lys Asp Leu Leu 
    50                  55                  60                  


Phe Val Met Thr Arg Leu Gln Val Arg Met Tyr Glu Tyr Pro Lys Trp 
65                  70                  75                  80  


Gly Asp Val Val Ala Val Glu Thr Tyr Phe Thr Glu Glu Gly Arg Leu 
                85                  90                  95      


Ala Phe Arg Arg Glu Trp Lys Leu Met Asp Val Ala Thr Gly Lys Leu 
            100                 105                 110         


Leu Gly Ala Gly Thr Ser Thr Trp Val Thr Ile Asn Thr Ala Thr Arg 
        115                 120                 125             


Arg Leu Ser Lys Leu Pro Glu Asp Val Arg Lys Arg Phe Leu Arg Phe 
    130                 135                 140                 


Ala Pro Pro Ser Ser Val His Ile Leu Pro Pro Glu Glu Thr Lys Lys 
145                 150                 155                 160 


Lys Leu Gln Asp Met Glu Leu Pro Gly Gln Val Gln Ser Ala Gln Gln 
                165                 170                 175     


Val Ala Arg Arg Ala Asp Met Asp Met Asn Gly His Ile Asn Asn Val 
            180                 185                 190         


Thr Tyr Leu Ala Trp Thr Leu Glu Ser Leu Pro Glu Arg Val Met Ser 
        195                 200                 205             


Gly Gly Tyr Lys Met Gln Glu Ile Glu Leu Asp Phe Lys Ala Glu Cys 
    210                 215                 220                 


Thr Ala Gly Asn Ala Ile Glu Ala His Cys Asn Pro Leu Asp Asp His 
225                 230                 235                 240 


Ser Ala Ser Phe Val Gly Pro Ala Pro Ala Asn Gly Asn Gly Asn Gly 
                245                 250                 255     


His Ala Ala Glu Pro Ala Ala Asp Ser Ala Pro Leu Tyr Phe Leu Ser 
            260                 265                 270         


Met Leu Gln Lys Cys Asp Glu Asn Gly Cys Thr Glu Leu Val Arg Ala 
        275                 280                 285             


Arg Thr Thr Trp Ser Arg Thr Leu Glu Gly Ala Lys Pro Ala Pro Pro 
    290                 295                 300                 


Pro Leu Ser Glu Leu Ser Ala Ala Gln Gly Thr Gly Glu Asn Leu Tyr 
305                 310                 315                 320 


Phe Gln Gly Ser Gly Gly Gly Gly Ser Asp Tyr Lys Asp Asp Asp Asp 
                325                 330                 335     


Lys Gly Thr Gly 
            340 


<210>  28
<211>  1503
<212>  DNA
<213>  Ricinus communis


<220>
<221>  misc_feature
<223>  3-keto-acyl-ACP synthase II N-terminal truncation

<220>
<221>  misc_feature
<222>  (1420)..(1500)
<223>  FLAG

<400>  28
atggtaccat ttgaaccatg ttcacactac tatagttcaa acggtttatt tccaaacact       60

cctttattac ctaaacgtca tcctagatta catcatcgtt tacctcgttc tggagaagct      120

atggctgttg ctgttcaacc tgaaaaagaa gtagcaacta acaaaaaacc attaatgaaa      180

caaagacgtg ttgttgttac tggtatgggt gtagtttctc cattaggtca cgatatagat      240

gtttactaca ataatcttct tgatggttct tcaggtattt cacaaattga ttcttttgac      300

tgcgctcaat ttccaacacg tattgcagga gaaatcaaat ctttctcaac agatggttgg      360

gttgctccta aattatcaaa acgtatggac aaatttatgt tatatatgct tactgctgga      420

aaaaaagcat tagcagatgg tggaataaca gaagatatga tggatgaatt agataaagct      480

cgttgcggtg ttttaattgg tagtgcaatg ggtggaatga aagtatttaa tgatgctatc      540

gaagcacttc gtatatctta ccgtaaaatg aacccatttt gcgtaccttt cgcaactaca      600

aatatgggtt cagcaatgtt agcaatggat cttggttgga tgggtcctaa ctattcaatt      660

tcaactgctt gtgctacaag taatttttgc atccttaatg ctgcaaacca cattattcgt      720

ggtgaagctg atattatgtt atgtggtgga tctgatgctg caattattcc tattggatta      780

ggtggttttg tagcatgtcg tgctttatct caacgtaacg acgatcctac aaaagcaagt      840

cgtccttggg atatgaatcg tgatggtttc gttatgggtg aaggtgctgg tgtattatta      900

cttgaagaat tagaacatgc taaaaaacgt ggtgcaaaca tttatgcaga gttcttaggt      960

ggtagtttta catgtgatgc ataccacatg acagagccac gtcctgatgg tgtaggtgtt     1020

atactttgta ttgaaaaagc attagcacgt tcaggtgttt ctaaagaaga ggttaactac     1080

attaatgcac atgcaacatc aacacctgct ggtgatttaa aagaatatga ggctttaatg     1140

cgttgttttt ctcaaaaccc tgatttacgt gttaattcaa caaaatcaat gattggtcat     1200

ttattaggtg ctgcaggcgc tgtagaagct attgctacta ttcaagctat tcgtacaggt     1260

tgggttcacc caaacattaa tcttgaaaat ccagaagaag gtgttgacac taaagtttta     1320

gttggtccaa aaaaagaacg tttagacatt aaagtagctc tttcaaattc atttggtttt     1380

ggaggtcaca atagttcaat tatcttcgca ccatataaag gtaccggtga aaacttatac     1440

tttcaaggct caggtggtgg tggttctgac tataaagatg atgacgataa aggaaccggt     1500

taa                                                                   1503


<210>  29
<211>  500
<212>  PRT
<213>  Ricinus communis


<220>
<221>  MISC_FEATURE
<223>  3-keto-acyl-ACP synthase II N-terminal truncation

<220>
<221>  MISC_FEATURE
<222>  (474)..(500)
<223>  FLAG

<400>  29

Met Val Pro Phe Glu Pro Cys Ser His Tyr Tyr Ser Ser Asn Gly Leu 
1               5                   10                  15      


Phe Pro Asn Thr Pro Leu Leu Pro Lys Arg His Pro Arg Leu His His 
            20                  25                  30          


Arg Leu Pro Arg Ser Gly Glu Ala Met Ala Val Ala Val Gln Pro Glu 
        35                  40                  45              


Lys Glu Val Ala Thr Asn Lys Lys Pro Leu Met Lys Gln Arg Arg Val 
    50                  55                  60                  


Val Val Thr Gly Met Gly Val Val Ser Pro Leu Gly His Asp Ile Asp 
65                  70                  75                  80  


Val Tyr Tyr Asn Asn Leu Leu Asp Gly Ser Ser Gly Ile Ser Gln Ile 
                85                  90                  95      


Asp Ser Phe Asp Cys Ala Gln Phe Pro Thr Arg Ile Ala Gly Glu Ile 
            100                 105                 110         


Lys Ser Phe Ser Thr Asp Gly Trp Val Ala Pro Lys Leu Ser Lys Arg 
        115                 120                 125             


Met Asp Lys Phe Met Leu Tyr Met Leu Thr Ala Gly Lys Lys Ala Leu 
    130                 135                 140                 


Ala Asp Gly Gly Ile Thr Glu Asp Met Met Asp Glu Leu Asp Lys Ala 
145                 150                 155                 160 


Arg Cys Gly Val Leu Ile Gly Ser Ala Met Gly Gly Met Lys Val Phe 
                165                 170                 175     


Asn Asp Ala Ile Glu Ala Leu Arg Ile Ser Tyr Arg Lys Met Asn Pro 
            180                 185                 190         


Phe Cys Val Pro Phe Ala Thr Thr Asn Met Gly Ser Ala Met Leu Ala 
        195                 200                 205             


Met Asp Leu Gly Trp Met Gly Pro Asn Tyr Ser Ile Ser Thr Ala Cys 
    210                 215                 220                 


Ala Thr Ser Asn Phe Cys Ile Leu Asn Ala Ala Asn His Ile Ile Arg 
225                 230                 235                 240 


Gly Glu Ala Asp Ile Met Leu Cys Gly Gly Ser Asp Ala Ala Ile Ile 
                245                 250                 255     


Pro Ile Gly Leu Gly Gly Phe Val Ala Cys Arg Ala Leu Ser Gln Arg 
            260                 265                 270         


Asn Asp Asp Pro Thr Lys Ala Ser Arg Pro Trp Asp Met Asn Arg Asp 
        275                 280                 285             


Gly Phe Val Met Gly Glu Gly Ala Gly Val Leu Leu Leu Glu Glu Leu 
    290                 295                 300                 


Glu His Ala Lys Lys Arg Gly Ala Asn Ile Tyr Ala Glu Phe Leu Gly 
305                 310                 315                 320 


Gly Ser Phe Thr Cys Asp Ala Tyr His Met Thr Glu Pro Arg Pro Asp 
                325                 330                 335     


Gly Val Gly Val Ile Leu Cys Ile Glu Lys Ala Leu Ala Arg Ser Gly 
            340                 345                 350         


Val Ser Lys Glu Glu Val Asn Tyr Ile Asn Ala His Ala Thr Ser Thr 
        355                 360                 365             


Pro Ala Gly Asp Leu Lys Glu Tyr Glu Ala Leu Met Arg Cys Phe Ser 
    370                 375                 380                 


Gln Asn Pro Asp Leu Arg Val Asn Ser Thr Lys Ser Met Ile Gly His 
385                 390                 395                 400 


Leu Leu Gly Ala Ala Gly Ala Val Glu Ala Ile Ala Thr Ile Gln Ala 
                405                 410                 415     


Ile Arg Thr Gly Trp Val His Pro Asn Ile Asn Leu Glu Asn Pro Glu 
            420                 425                 430         


Glu Gly Val Asp Thr Lys Val Leu Val Gly Pro Lys Lys Glu Arg Leu 
        435                 440                 445             


Asp Ile Lys Val Ala Leu Ser Asn Ser Phe Gly Phe Gly Gly His Asn 
    450                 455                 460                 


Ser Ser Ile Ile Phe Ala Pro Tyr Lys Gly Thr Gly Glu Asn Leu Tyr 
465                 470                 475                 480 


Phe Gln Gly Ser Gly Gly Gly Gly Ser Asp Tyr Lys Asp Asp Asp Asp 
                485                 490                 495     


Lys Gly Thr Gly 
            500 


<210>  30
<211>  1050
<212>  DNA
<213>  Umbellularia californica


<220>
<221>  misc_feature
<223>  Thioesterase N-terminal trunication

<220>
<221>  misc_feature
<222>  (967)..(1047)
<223>  FLAG

<400>  30
atggtaccat ctatgttatt cgcagttatc acaactattt tctctgctgc tgaaaaacaa       60

tggacaaatt tagaatggaa accaaaacca aaattacctc aattattaga tgaccatttt      120

ggtcttcatg gcttagtttt tagacgtact ttcgctattc gttcttatga agttggccca      180

gatcgttcta catcaatttt agcagttatg aaccacatgc aagaagctac attaaaccat      240

gcaaaatcag ttggaatttt aggtgatggc tttggaacaa cattagagat gtcaaaacgt      300

gatttaatgt gggttgttcg tcgtactcac gtagctgttg aaagatatcc aacttggggc      360

gatactgtag aagttgaatg ttggattggt gcttcaggta acaatggtat gcgtcgtgat      420

ttcttagtac gtgattgtaa aacaggtgag attttaactc gttgtacatc tttatcagta      480

ttaatgaaca cacgtacacg tagattatct acaattccag acgaagtacg tggcgaaatt      540

ggtcctgctt ttattgataa cgttgctgtt aaagatgatg aaataaaaaa acttcaaaaa      600

cttaatgatt ctactgcaga ttatattcaa ggcggtttaa ctcctcgttg gaatgactta      660

gacgttaacc aacatgtaaa caatttaaaa tatgttgctt gggtatttga aacagttcca      720

gattctattt ttgaatcaca tcatatttca agttttactt tagagtatcg tagagaatgt      780

actcgtgatt cagttttacg ttctttaaca acagtatcag gtggttctag tgaagctggt      840

ttagtatgtg atcacttatt acaattagaa ggcggttctg aagtattacg tgctcgtaca      900

gaatggcgtc ctaaattaac agatagtttt cgtggtattt ctgtaatccc tgctgaacct      960

cgtgtaggta ccggtgagaa tttatatttc caaggtagtg gaggtggcgg tagtgattac     1020

aaagacgatg atgacaaagg aaccggttaa                                      1050


<210>  31
<211>  349
<212>  PRT
<213>  Umbellularia californica


<220>
<221>  MISC_FEATURE
<223>  Thioesterase N-terminal truncation

<220>
<221>  MISC_FEATURE
<222>  (323)..(349)
<223>  FLAG

<400>  31

Met Val Pro Ser Met Leu Phe Ala Val Ile Thr Thr Ile Phe Ser Ala 
1               5                   10                  15      


Ala Glu Lys Gln Trp Thr Asn Leu Glu Trp Lys Pro Lys Pro Lys Leu 
            20                  25                  30          


Pro Gln Leu Leu Asp Asp His Phe Gly Leu His Gly Leu Val Phe Arg 
        35                  40                  45              


Arg Thr Phe Ala Ile Arg Ser Tyr Glu Val Gly Pro Asp Arg Ser Thr 
    50                  55                  60                  


Ser Ile Leu Ala Val Met Asn His Met Gln Glu Ala Thr Leu Asn His 
65                  70                  75                  80  


Ala Lys Ser Val Gly Ile Leu Gly Asp Gly Phe Gly Thr Thr Leu Glu 
                85                  90                  95      


Met Ser Lys Arg Asp Leu Met Trp Val Val Arg Arg Thr His Val Ala 
            100                 105                 110         


Val Glu Arg Tyr Pro Thr Trp Gly Asp Thr Val Glu Val Glu Cys Trp 
        115                 120                 125             


Ile Gly Ala Ser Gly Asn Asn Gly Met Arg Arg Asp Phe Leu Val Arg 
    130                 135                 140                 


Asp Cys Lys Thr Gly Glu Ile Leu Thr Arg Cys Thr Ser Leu Ser Val 
145                 150                 155                 160 


Leu Met Asn Thr Arg Thr Arg Arg Leu Ser Thr Ile Pro Asp Glu Val 
                165                 170                 175     


Arg Gly Glu Ile Gly Pro Ala Phe Ile Asp Asn Val Ala Val Lys Asp 
            180                 185                 190         


Asp Glu Ile Lys Lys Leu Gln Lys Leu Asn Asp Ser Thr Ala Asp Tyr 
        195                 200                 205             


Ile Gln Gly Gly Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln 
    210                 215                 220                 


His Val Asn Asn Leu Lys Tyr Val Ala Trp Val Phe Glu Thr Val Pro 
225                 230                 235                 240 


Asp Ser Ile Phe Glu Ser His His Ile Ser Ser Phe Thr Leu Glu Tyr 
                245                 250                 255     


Arg Arg Glu Cys Thr Arg Asp Ser Val Leu Arg Ser Leu Thr Thr Val 
            260                 265                 270         


Ser Gly Gly Ser Ser Glu Ala Gly Leu Val Cys Asp His Leu Leu Gln 
        275                 280                 285             


Leu Glu Gly Gly Ser Glu Val Leu Arg Ala Arg Thr Glu Trp Arg Pro 
    290                 295                 300                 


Lys Leu Thr Asp Ser Phe Arg Gly Ile Ser Val Ile Pro Ala Glu Pro 
305                 310                 315                 320 


Arg Val Gly Thr Gly Glu Asn Leu Tyr Phe Gln Gly Ser Gly Gly Gly 
                325                 330                 335     


Gly Ser Asp Tyr Lys Asp Asp Asp Asp Lys Gly Thr Gly 
            340                 345                 


<210>  32
<211>  1050
<212>  DNA
<213>  Cinnamomum camphora


<220>
<221>  misc_feature
<223>  Thioesterase N-terminal truncation

<220>
<221>  misc_feature
<222>  (967)..(1047)
<223>  FLAG

<400>  32
atggtaccat caatgttatt tgctgttatt actacaatct tttctgctgc tgaaaaacaa       60

tggacaaatt tagaatggaa accaaaacca aatcctccac aattacttga cgaccacttt      120

ggtcctcatg gtttagtttt tcgtcgtaca tttgcaattc gtagttatga agttggccca      180

gaccgttcaa caagtattgt tgcagttatg aatcatttac aagaagcagc tttaaaccat      240

gcaaaaagtg taggtatttt aggtgatgga ttcggtacta cattagaaat gtcaaaacgt      300

gatcttattt gggttgttaa acgtactcac gtagcagtag aacgttaccc agcttggggt      360

gatacagtag aagtagaatg ttgggttggt gcatcaggta acaatggtcg tcgtcacgac      420

tttcttgtac gtgactgcaa aactggtgaa atcttaacac gttgtacatc actttcagtt      480

atgatgaata ctcgtacacg tcgtttatca aaaattccag aggaggtacg tggtgagatt      540

ggtccagctt ttattgataa tgtagcagtt aaagatgaag aaataaaaaa accacaaaaa      600

ttaaacgatt caacagcaga ctatatccag ggaggtctta ctccacgttg gaacgactta      660

gatattaatc aacatgtaaa caatataaaa tatgtagact ggatattaga aactgttcct      720

gattcaattt ttgaaagtca ccacattagt agttttacta tagagtatcg tcgtgaatgt      780

actatggatt cagttttaca atcattaact actgtttcag gaggttcatc agaagcagga      840

ttagtttgtg aacatttact tcaattagaa ggtggttcag aagttttacg tgctaaaaca      900

gaatggcgtc caaaattaac agattctttc cgtggtataa gtgttattcc agcagaatca      960

tctgtaggta ccggtgaaaa tttatatttt caaggttctg gcggtggtgg tagtgactat     1020

aaagatgatg atgataaagg aaccggttaa                                      1050


<210>  33
<211>  349
<212>  PRT
<213>  Cinnamomum camphora


<220>
<221>  MISC_FEATURE
<223>  Thioesterase N-terminal trunication

<220>
<221>  MISC_FEATURE
<222>  (323)..(349)
<223>  FLAG

<400>  33

Met Val Pro Ser Met Leu Phe Ala Val Ile Thr Thr Ile Phe Ser Ala 
1               5                   10                  15      


Ala Glu Lys Gln Trp Thr Asn Leu Glu Trp Lys Pro Lys Pro Asn Pro 
            20                  25                  30          


Pro Gln Leu Leu Asp Asp His Phe Gly Pro His Gly Leu Val Phe Arg 
        35                  40                  45              


Arg Thr Phe Ala Ile Arg Ser Tyr Glu Val Gly Pro Asp Arg Ser Thr 
    50                  55                  60                  


Ser Ile Val Ala Val Met Asn His Leu Gln Glu Ala Ala Leu Asn His 
65                  70                  75                  80  


Ala Lys Ser Val Gly Ile Leu Gly Asp Gly Phe Gly Thr Thr Leu Glu 
                85                  90                  95      


Met Ser Lys Arg Asp Leu Ile Trp Val Val Lys Arg Thr His Val Ala 
            100                 105                 110         


Val Glu Arg Tyr Pro Ala Trp Gly Asp Thr Val Glu Val Glu Cys Trp 
        115                 120                 125             


Val Gly Ala Ser Gly Asn Asn Gly Arg Arg His Asp Phe Leu Val Arg 
    130                 135                 140                 


Asp Cys Lys Thr Gly Glu Ile Leu Thr Arg Cys Thr Ser Leu Ser Val 
145                 150                 155                 160 


Met Met Asn Thr Arg Thr Arg Arg Leu Ser Lys Ile Pro Glu Glu Val 
                165                 170                 175     


Arg Gly Glu Ile Gly Pro Ala Phe Ile Asp Asn Val Ala Val Lys Asp 
            180                 185                 190         


Glu Glu Ile Lys Lys Pro Gln Lys Leu Asn Asp Ser Thr Ala Asp Tyr 
        195                 200                 205             


Ile Gln Gly Gly Leu Thr Pro Arg Trp Asn Asp Leu Asp Ile Asn Gln 
    210                 215                 220                 


His Val Asn Asn Ile Lys Tyr Val Asp Trp Ile Leu Glu Thr Val Pro 
225                 230                 235                 240 


Asp Ser Ile Phe Glu Ser His His Ile Ser Ser Phe Thr Ile Glu Tyr 
                245                 250                 255     


Arg Arg Glu Cys Thr Met Asp Ser Val Leu Gln Ser Leu Thr Thr Val 
            260                 265                 270         


Ser Gly Gly Ser Ser Glu Ala Gly Leu Val Cys Glu His Leu Leu Gln 
        275                 280                 285             


Leu Glu Gly Gly Ser Glu Val Leu Arg Ala Lys Thr Glu Trp Arg Pro 
    290                 295                 300                 


Lys Leu Thr Asp Ser Phe Arg Gly Ile Ser Val Ile Pro Ala Glu Ser 
305                 310                 315                 320 


Ser Val Gly Thr Gly Glu Asn Leu Tyr Phe Gln Gly Ser Gly Gly Gly 
                325                 330                 335     


Gly Ser Asp Tyr Lys Asp Asp Asp Asp Lys Gly Thr Gly 
            340                 345                 


<210>  34
<211>  1071
<212>  DNA
<213>  Chlamydomonas reinhardtii


<220>
<221>  misc_feature
<223>  Thioesterase N-terminal truncation

<220>
<221>  misc_feature
<222>  (988)..(1068)
<223>  FLAG

<400>  34
atggtaccag atgtagttat tcctaaattt cctgctccag ctgcaggtgc attcttacct       60

gataaacgtt catttagaga agagcataga attagaggat atgaggtatc tccagaccaa      120

cgtgctacaa ttgttacagt tgctaactta ttacaagaag tagctggaaa tcatgctgta      180

ggcatgtggg gtcgtactga tgaaggtttc gctagtttac catctatgaa agacttatta      240

ttcgtaatga ctcgtcttca agttcgtatg tatgaatacc caaaatgggg tgacgttgtt      300

gcagtagaaa cttatttcac tgaagaaggt cgtttagctt ttcgtcgtga gtggaaatta      360

atggacgtag ctacaggtaa attattaggc gctggtactt caacttgggt aacaataaat      420

acagcaactc gtcgtttatc aaaattacca gaagatgttc gtaaacgttt cttacgtttt      480

gctcctcctt catctgtaca tattttacct ccagaagaaa ctaaaaaaaa acttcaagat      540

atggaattac caggacaagt tcaatctgct caacaagttg ctcgtcgtgc tgatatggac      600

atgaacggcc acattaacaa tgttacatat cttgcttgga cattagaatc attacctgag      660

cgtgtaatgt caggtggcta taaaatgcaa gaaattgaat tagatttcaa agctgagtgc      720

actgcaggta acgctattga ggcacattgt aaccctttag atgatcatag tgcttcattt      780

gttggtccag caccagctaa tggcaatggt aatggtcacg ctgcagaacc agcagctgat      840

tcagctccac tttactttct ttctatgctt caaaaatgtg atgaaaatgg ttgcacagaa      900

ttagtacgtg ctcgtacaac ttggagtcgt acacttgaag gtgcaaaacc tgcaccacca      960

ccattatctg aattatcagc agctcaaggt accggtgaaa atttatattt tcaaggttca     1020

ggaggtggtg gctctgatta taaagatgat gatgataaag gaaccggtta a              1071


<210>  35
<211>  356
<212>  PRT
<213>  Chlamydomonas reinhardtii


<220>
<221>  MISC_FEATURE
<223>  Thioesterase N-terminal truncation

<220>
<221>  MISC_FEATURE
<222>  (330)..(356)
<223>  FLAG

<400>  35

Met Val Pro Asp Val Val Ile Pro Lys Phe Pro Ala Pro Ala Ala Gly 
1               5                   10                  15      


Ala Phe Leu Pro Asp Lys Arg Ser Phe Arg Glu Glu His Arg Ile Arg 
            20                  25                  30          


Gly Tyr Glu Val Ser Pro Asp Gln Arg Ala Thr Ile Val Thr Val Ala 
        35                  40                  45              


Asn Leu Leu Gln Glu Val Ala Gly Asn His Ala Val Gly Met Trp Gly 
    50                  55                  60                  


Arg Thr Asp Glu Gly Phe Ala Ser Leu Pro Ser Met Lys Asp Leu Leu 
65                  70                  75                  80  


Phe Val Met Thr Arg Leu Gln Val Arg Met Tyr Glu Tyr Pro Lys Trp 
                85                  90                  95      


Gly Asp Val Val Ala Val Glu Thr Tyr Phe Thr Glu Glu Gly Arg Leu 
            100                 105                 110         


Ala Phe Arg Arg Glu Trp Lys Leu Met Asp Val Ala Thr Gly Lys Leu 
        115                 120                 125             


Leu Gly Ala Gly Thr Ser Thr Trp Val Thr Ile Asn Thr Ala Thr Arg 
    130                 135                 140                 


Arg Leu Ser Lys Leu Pro Glu Asp Val Arg Lys Arg Phe Leu Arg Phe 
145                 150                 155                 160 


Ala Pro Pro Ser Ser Val His Ile Leu Pro Pro Glu Glu Thr Lys Lys 
                165                 170                 175     


Lys Leu Gln Asp Met Glu Leu Pro Gly Gln Val Gln Ser Ala Gln Gln 
            180                 185                 190         


Val Ala Arg Arg Ala Asp Met Asp Met Asn Gly His Ile Asn Asn Val 
        195                 200                 205             


Thr Tyr Leu Ala Trp Thr Leu Glu Ser Leu Pro Glu Arg Val Met Ser 
    210                 215                 220                 


Gly Gly Tyr Lys Met Gln Glu Ile Glu Leu Asp Phe Lys Ala Glu Cys 
225                 230                 235                 240 


Thr Ala Gly Asn Ala Ile Glu Ala His Cys Asn Pro Leu Asp Asp His 
                245                 250                 255     


Ser Ala Ser Phe Val Gly Pro Ala Pro Ala Asn Gly Asn Gly Asn Gly 
            260                 265                 270         


His Ala Ala Glu Pro Ala Ala Asp Ser Ala Pro Leu Tyr Phe Leu Ser 
        275                 280                 285             


Met Leu Gln Lys Cys Asp Glu Asn Gly Cys Thr Glu Leu Val Arg Ala 
    290                 295                 300                 


Arg Thr Thr Trp Ser Arg Thr Leu Glu Gly Ala Lys Pro Ala Pro Pro 
305                 310                 315                 320 


Pro Leu Ser Glu Leu Ser Ala Ala Gln Gly Thr Gly Glu Asn Leu Tyr 
                325                 330                 335     


Phe Gln Gly Ser Gly Gly Gly Gly Ser Asp Tyr Lys Asp Asp Asp Asp 
            340                 345                 350         


Lys Gly Thr Gly 
        355     


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
cgttttgctc ctccttcatc                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
ccacctcctg aaccttgaaa                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
tcgttttaca tggccacaaa                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
ccacctcctg aaccttgaaa                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
gtcatgcctt aggcggttta                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  41
caccaccgct accttgaaaa                                                   20


