                         SEQUENCE LISTING

<110>  Commonwealth Scientific and Industrial Research 
       Organisation
 
<120>  Enzymes and methods for degrading s-triazines and diazines

<130>  508352

<150>  US 61/094,044
<151>  2008-09-03

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  456
<212>  PRT
<213>  Arthrobacter aurescens

<400>  1

Met Ile Leu Ile Arg Gly Leu Thr Arg Val Ile Thr Phe Asp Asp Gln 
1               5                   10                  15      


Glu Arg Glu Leu Glu Asp Ala Asp Ile Leu Ile Asp Gly Pro Lys Ile 
            20                  25                  30          


Val Ala Val Gly Lys Asp Leu Ser Asp Arg Ser Val Ser Arg Thr Ile 
        35                  40                  45              


Asp Gly Arg Gly Met Ile Ala Leu Pro Gly Leu Ile Asn Ser His Gln 
    50                  55                  60                  


His Leu Tyr Glu Gly Ala Met Arg Ala Ile Pro Gln Leu Glu Arg Val 
65                  70                  75                  80  


Thr Met Ala Ser Trp Leu Glu Gly Val Leu Thr Arg Ser Ala Gly Trp 
                85                  90                  95      


Trp Arg Asp Gly Lys Phe Gly Pro Asp Val Ile Arg Glu Val Ala Arg 
            100                 105                 110         


Ala Val Leu Leu Glu Ser Leu Leu Gly Gly Ile Thr Thr Val Ala Asp 
        115                 120                 125             


Gln His Leu Phe Phe Pro Gly Ala Thr Ala Asp Ser Tyr Ile Asp Ala 
    130                 135                 140                 


Thr Ile Glu Ala Ala Thr Asp Leu Gly Ile Arg Phe His Ala Ala Arg 
145                 150                 155                 160 


Ser Ser Met Thr Leu Gly Lys Ser Glu Gly Gly Phe Cys Asp Asp Leu 
                165                 170                 175     


Phe Val Glu Pro Val Asp Arg Val Val Gln His Cys Leu Gly Leu Ile 
            180                 185                 190         


Asp Gln Tyr His Glu Pro Glu Pro Phe Gly Met Val Arg Ile Ala Leu 
        195                 200                 205             


Gly Pro Cys Gly Val Pro Tyr Asp Lys Pro Glu Leu Phe Glu Ala Phe 
    210                 215                 220                 


Ala Gln Met Ala Ala Asp Tyr Asp Val Arg Leu His Thr His Phe Tyr 
225                 230                 235                 240 


Glu Pro Leu Asp Ala Gly Met Ser Asp His Leu Tyr Gly Met Thr Pro 
                245                 250                 255     


Trp Arg Phe Leu Glu Lys His Gly Trp Ala Ser Asp Arg Val Trp Leu 
            260                 265                 270         


Ala His Ala Val Val Pro Pro Arg Glu Glu Ile Pro Glu Phe Ala Asp 
        275                 280                 285             


Ala Gly Val Ala Ile Ala His Leu Ile Ala Pro Asp Leu Arg Leu Gly 
    290                 295                 300                 


Trp Gly Leu Ala Pro Ile Arg Glu Tyr Leu Asp Ala Gly Ile Thr Val 
305                 310                 315                 320 


Gly Phe Gly Thr Thr Gly Ser Ala Ser Asn Asp Gly Gly Asn Leu Leu 
                325                 330                 335     


Gly Asp Leu Arg Leu Ala Ala Leu Ala His Arg Pro Ala Asp Pro Asn 
            340                 345                 350         


Glu Pro Glu Lys Trp Leu Ser Ala Arg Glu Leu Leu Arg Met Ala Thr 
        355                 360                 365             


Arg Gly Ser Ala Glu Cys Leu Gly Arg Pro Asp Leu Gly Val Leu Glu 
    370                 375                 380                 


Glu Gly Arg Ala Ala Asp Ile Ala Cys Trp Arg Leu Asp Gly Val Asp 
385                 390                 395                 400 


Arg Val Gly Val His Asp Pro Ala Ile Gly Leu Ile Met Thr Gly Leu 
                405                 410                 415     


Ser Asp Arg Ala Ser Leu Val Val Val Asn Gly Gln Val Leu Val Glu 
            420                 425                 430         


Asn Glu Arg Pro Val Leu Ala Asp Leu Glu Arg Ile Val Ala Asn Thr 
        435                 440                 445             


Thr Ala Leu Ile Pro Lys Asn Leu 
    450                 455     


<210>  2
<211>  1371
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimised open reading frame encoding TrzN

<400>  2
atgattctga tccgtggtct gacccgcgtt atcaccttcg atgatcagga gcgtgaactg       60

gaagacgcgg atattctgat tgacggtccg aagattgtcg ctgtgggtaa agatttaagc      120

gatcgtagcg tgagccgcac gatcgatggt cgcggcatga ttgccctgcc gggtctgatc      180

aatagccacc agcatctgta tgagggcgcg atgcgcgcta ttccgcaact ggaacgcgtt      240

acgatggcga gctggttaga gggcgtcctg actcgtagcg cgggttggtg gcgtgacggt      300

aaatttggtc cggacgtcat tcgcgaggtc gcacgtgccg tcctgctgga aagcctgctg      360

ggcggtatca ccaccgtcgc cgatcagcat ctgttttttc caggtgcaac cgcagatagc      420

tatattgatg caaccatcga agccgcgacc gacctgggta ttcgctttca tgccgcgcgc      480

agcagcatga ctctgggtaa gagcgaaggt ggcttctgtg atgacctgtt tgtggaacca      540

gtggatcgtg tggttcagca ttgcctgggc ctgattgatc agtaccacga gccggaacca      600

tttggcatgg tccgcattgc actgggtccg tgcggcgttc cgtatgataa accagaactg      660

tttgaggcgt ttgcccagat ggccgccgac tacgacgtgc gcctgcatac gcatttttat      720

gaaccgctgg acgcgggtat gagcgatcat ctgtatggca tgacgccgtg gcgtttctta      780

gagaagcatg gctgggccag cgaccgcgtt tggctggcac atgccgttgt gccgccgcgt      840

gaagagattc cagaatttgc ggatgccggc gttgcaattg cccacctgat tgcgccggat      900

ctgcgtctgg gctggggtct ggcaccgatt cgtgaatatc tggatgcggg catcaccgtg      960

ggtttcggaa ccaccggtag cgccagcaac gacggtggca acctgttagg tgatctgcgc     1020

ctggcagccc tggcgcatcg cccggcggac ccgaatgaac cagagaagtg gctgagcgcc     1080

cgtgaactgc tgcgcatggc tacgcgcggt agcgcagagt gtctgggccg cccggatctg     1140

ggtgtgctgg aagagggtcg tgccgcagac atcgcgtgct ggcgcctgga tggcgtggac     1200

cgcgtgggtg tgcacgatcc ggctatcggc ctgatcatga ccggtctgag cgaccgcgca     1260

agcctggttg tcgtcaacgg ccaggttctg gtcgaaaatg aacgtccggt tctggcggat     1320

ctggaacgca tcgtggctaa taccacggcc ctgattccga aaaatctgta a              1371


<210>  3
<211>  1371
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimised open reading frame encoding TrzN with 156th codon
       change from TTT to TTC

<400>  3
atgattctga tccgtggtct gacccgcgtt atcaccttcg atgatcagga gcgtgaactg       60

gaagacgcgg atattctgat tgacggtccg aagattgtcg ctgtgggtaa agatttaagc      120

gatcgtagcg tgagccgcac gatcgatggt cgcggcatga ttgccctgcc gggtctgatc      180

aatagccacc agcatctgta tgagggcgcg atgcgcgcta ttccgcaact ggaacgcgtt      240

acgatggcga gctggttaga gggcgtcctg actcgtagcg cgggttggtg gcgtgacggt      300

aaatttggtc cggacgtcat tcgcgaggtc gcacgtgccg tcctgctgga aagcctgctg      360

ggcggtatca ccaccgtcgc cgatcagcat ctgttttttc caggtgcaac cgcagatagc      420

tatattgatg caaccatcga agccgcgacc gacctgggta ttcgcttcca tgccgcgcgc      480

agcagcatga ctctgggtaa gagcgaaggt ggcttctgtg atgacctgtt tgtggaacca      540

gtggatcgtg tggttcagca ttgcctgggc ctgattgatc agtaccacga gccggaacca      600

tttggcatgg tccgcattgc actgggtccg tgcggcgttc cgtatgataa accagaactg      660

tttgaggcgt ttgcccagat ggccgccgac tacgacgtgc gcctgcatac gcatttttat      720

gaaccgctgg acgcgggtat gagcgatcat ctgtatggca tgacgccgtg gcgtttctta      780

gagaagcatg gctgggccag cgaccgcgtt tggctggcac atgccgttgt gccgccgcgt      840

gaagagattc cagaatttgc ggatgccggc gttgcaattg cccacctgat tgcgccggat      900

ctgcgtctgg gctggggtct ggcaccgatt cgtgaatatc tggatgcggg catcaccgtg      960

ggtttcggaa ccaccggtag cgccagcaac gacggtggca acctgttagg tgatctgcgc     1020

ctggcagccc tggcgcatcg cccggcggac ccgaatgaac cagagaagtg gctgagcgcc     1080

cgtgaactgc tgcgcatggc tacgcgcggt agcgcagagt gtctgggccg cccggatctg     1140

ggtgtgctgg aagagggtcg tgccgcagac atcgcgtgct ggcgcctgga tggcgtggac     1200

cgcgtgggtg tgcacgatcc ggctatcggc ctgatcatga ccggtctgag cgaccgcgca     1260

agcctggttg tcgtcaacgg ccaggttctg gtcgaaaatg aacgtccggt tctggcggat     1320

ctggaacgca tcgtggctaa taccacggcc ctgattccga aaaatctgta a              1371


<210>  4
<211>  1371
<212>  DNA
<213>  Arthrobacter aurescens


<220>
<221>  misc_feature
<222>  (792)..(792)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1241)..(1241)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1312)..(1312)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1321)..(1321)
<223>  n is a, c, g, or t

<400>  4
atgatcctga tccgcggact gacacgtgtc atcactttcg acgatcaaga acgcgagttg       60

gaagacgccg acattctgat cgacgggccg aagatcgtcg ccgtcggaaa ggacctgtcg      120

gatcgcagcg tttcacggac aatcgacggg cgtggaatga ttgcgctgcc aggtctcatc      180

aactcgcacc agcacctgta cgaaggcgca atgcgggcga ttccccaact cgagcgggtc      240

acgatggcca gttggctcga aggcgtcctg accagaagcg caggatggtg gcgcgacggg      300

aagttcggtc cagatgtcat ccgggaggtt gcgcgcgccg tcctcctcga gtcgctcctc      360

ggtggcatca ccacggtcgc cgaccagcac ctattcttcc ccggtgccac ggccgatagc      420

tatatcgacg caaccatcga ggccgccacc gacctcggga tccggttcca tgcggcaagg      480

tcatcgatga cgctcggcaa gagcgaaggt ggcttctgcg acgacctgtt cgtcgagccc      540

gtcgacagag tcgtccagca ctgtctgggt ctcatcgacc agtaccacga gccggaaccg      600

tttggaatgg ttcgaatcgc tcttggaccc tgcggcgtcc cctacgacaa gcccgaactc      660

ttcgaagcgt tcgcacagat ggcggcggac tacgacgtgc gcctccacac ccacttctac      720

gaaccgctcg acgcgggaat gagcgaccac ctctacggta tgacgccgtg gcgtttcctt      780

gagaagcacg gntgggccag cgaccgagta tggctggctc acgctgtggt acctccacgg      840

gaggagatcc ccgagttcgc cgacgcaggc gtcgcgatcg cgcatctcat tgcgccggac      900

cttcggttgg gctggggact cgcgcctatc cgggaatacc tcgacgccgg aatcacggtt      960

gggtttggaa cgaccgggtc cgccagcaac gatggcggga acctgcttgg cgatctgcgc     1020

ctggctgcgc tggcgcaccg ccccgctgac cccaacgagc ccgaaaagtg gctgtcggca     1080

cgcgagctgc tccgaatggc aaccagggga tcagcggagt gcctcggcag gcccgacctg     1140

ggcgtcctcg aggagggtcg tgctgcggac atcgcctgtt ggcgcctcga tggagtcgac     1200

cgggttggtg tgcacgaccc ggcgatcggc ctgatcatga ntggtctttc agaccgggca     1260

agcctggtgg tcgtgaatgg tcaggtgctc gtcgagaacg aacgacccgt tntggcagat     1320

ntcgaacgca tcgtcgcgaa tacgacagca ctcattccca agaacttgta g              1371


<210>  5
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  5
ccacaacata tgattctgat ccgtggtctg acccgcgtta tc                          42


<210>  6
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  6
cttcgaattc ttacagattt ttcggaatca gggccgtggt attag                       45


<210>  7
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (64)..(65)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  s = c or g

<400>  7
cgcacgatcg atggtcgcgg catgattgcc ctgccgggtc tgatcaatag ccaccagcat       60

ctgnnsgagg gc                                                           72


<210>  8
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  8
cagatgctgg tggctattga tcagacccgg cagggcaatc atgccgcgac catcgatcgt       60

gc                                                                      62


<210>  9
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  s = c or g

<400>  9
gcgagctggt tagagggcgt cctgnnscgt agcgcgggtt ggtggcgt                    48


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  10
acgccaccaa cccgcgctac g                                                 21


<210>  11
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (51)..(52)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (53)..(53)
<223>  s = c or g

<400>  11
tcctgctgga aagcctgctg ggcggtatca ccaccgtcgc cgatcagcat nnsttttttc       60

caggtgcaac cgc                                                          73


<210>  12
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  12
atgctgatcg gcgacggtgg tgataccgcc cagcaggctt tccagcagga                  50


<210>  13
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (55)..(56)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (57)..(57)
<223>  s = c or g

<400>  13
tatattgatg caaccatcga agccgcgacc gacctgggta ttcgctttca tgccnnscgc       60

agcagcatg                                                               69


<210>  14
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  14
ggcatgaaag cgaataccca ggtcggtcgc ggcttcgatg gttgcatcaa tata             54


<210>  15
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (49)..(49)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (50)..(50)
<223>  s = c or g

<400>  15
accatcgaag ccgcgaccga cctgggtatt cgctttcatg ccgcgcgcnn sagcatgact       60

ctgggtaag                                                               69


<210>  16
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  16
gcgcgcggca tgaaagcgaa tacccaggtc ggtcgcggct tcgatggt                    48


<210>  17
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  s = c or g

<400>  17
gaaccatttg gcatggtccg cattgcactg ggtnnstgcg gcgttccgta t                51


<210>  18
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  18
acccagtgca atgcggacca tgccaaatgg ttc                                    33


<210>  19
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  s = c or g

<400>  19
gtgcgcctgc atacgcattt ttatgaaccg nnsgacgcg                              39


<210>  20
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  20
cggttcataa aaatgcgtat gcaggcgcac                                        30


<210>  21
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  s = c or g

<400>  21
ctgcatacgc atttttatga accgctggac gcgnnsatga gc                          42


<210>  22
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  22
cgcgtccagc ggttcataaa aatgcgtatg cag                                    33


<210>  23
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  s = c or g

<400>  23
attccagaat ttgcggatgc cggcgttgca attnnscacc tgattgcgcc ggatctgcgt       60

c                                                                       61


<210>  24
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  24
aattgcaacg ccggcatccg caaattctgg aat                                    33


<210>  25
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  s = c or g

<400>  25
accggtagcg ccagcaacga cggtggcaac nnsttaggtg atctg                       45


<210>  26
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  26
gttgccaccg tcgttgctgg cgctaccggt                                        30


<210>  27
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (43)..(44)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  s = c or g

<400>  27
ttaggtgatc tgcgcctggc agccctggcg catcgcccgg cgnnsccgaa tgaa             54


<210>  28
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  28
cgccgggcga tgcgccaggg ctgccaggcg cagatcacct aa                          42


<210>  29
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  s = g or c

<400>  29
gctgtgggta aannsttaag cgatcgtag                                         29


<210>  30
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  s = g or c

<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  30
ctacgatcgc ttaasnnttt acccacagc                                         29


