                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  Nachweis und Verwendung niedermolekularer Modulatoren des 
       Klte-Menthol-Rezeptors TRPM8

<130>  M/49197-PCT

<160>  2     

<170>  PatentIn version 3.3

<210>  1
<211>  5621
<212>  DNA
<213>  Homo sapiens

<400>  1
aagaaaatcc tgcttgacaa aaaccgtcac ttaggaaaag atgtcctttc gggcagccag     60

gctcagcatg aggaacagaa ggaatgacac tctggacagc acccggaccc tgtactccag    120

cgcgtctcgg agcacagact tgtcttacag tgaaagcgac ttggtgaatt ttattcaagc    180

aaattttaag aaacgagaat gtgtcttctt taccaaagat tccaaggcca cggagaatgt    240

gtgcaagtgt ggctatgccc agagccagca catggaaggc acccagatca accaaagtga    300

gaaatggaac tacaagaaac acaccaagga atttcctacc gacgcctttg gggatattca    360

gtttgagaca ctggggaaga aagggaagta tatacgtctg tcctgcgaca cggacgcgga    420

aatcctttac gagctgctga cccagcactg gcacctgaaa acacccaacc tggtcatttc    480

tgtgaccggg ggcgccaaga acttcgccct gaagccgcgc atgcgcaaga tcttcagccg    540

gctcatctac atcgcgcagt ccaaaggtgc ttggattctc acgggaggca cccattatgg    600

cctgatgaag tacatcgggg aggtggtgag agataacacc atcagcagga gttcagagga    660

gaatattgtg gccattggca tagcagcttg gggcatggtc tccaaccggg acaccctcat    720

caggaattgc gatgctgagg gctatttttt agcccagtac cttatggatg acttcacaag    780

agatccactg tatatcctgg acaacaacca cacacatttg ctgctcgtgg acaatggctg    840

tcatggacat cccactgtcg aagcaaagct ccggaatcag ctagagaagt atatctctga    900

gcgcactatt caagattcca actatggtgg caagatcccc attgtgtgtt ttgcccaagg    960

aggtggaaaa gagactttga aagccatcaa tacctccatc aaaaataaaa ttccttgtgt   1020

ggtggtggaa ggctcgggcc agatcgctga tgtgatcgct agcctggtgg aggtggagga   1080

tgccctgaca tcttctgccg tcaaggagaa gctggtgcgc tttttacccc gcacggtgtc   1140

ccggctgcct gaggaggaga ctgagagttg gatcaaatgg ctcaaagaaa ttctcgaatg   1200

ttctcaccta ttaacagtta ttaaaatgga agaagctggg gatgaaattg tgagcaatgc   1260

catctcctac gctctataca aagccttcag caccagtgag caagacaagg ataactggaa   1320

tgggcagctg aagcttctgc tggagtggaa ccagctggac ttagccaatg atgagatttt   1380

caccaatgac cgccgatggg agtctgctga ccttcaagaa gtcatgttta cggctctcat   1440

aaaggacaga cccaagtttg tccgcctctt tctggagaat ggcttgaacc tacggaagtt   1500

tctcacccat gatgtcctca ctgaactctt ctccaaccac ttcagcacgc ttgtgtaccg   1560

gaatctgcag atcgccaaga attcctataa tgatgccctc ctcacgtttg tctggaaact   1620

ggttgcgaac ttccgaagag gcttccggaa ggaagacaga aatggccggg acgagatgga   1680

catagaactc cacgacgtgt ctcctattac tcggcacccc ctgcaagctc tcttcatctg   1740

ggccattctt cagaataaga aggaactctc caaagtcatt tgggagcaga ccaggggctg   1800

cactctggca gccctgggag ccagcaagct tctgaagact ctggccaaag tgaagaacga   1860

catcaatgct gctggggagt ccgaggagct ggctaatgag tacgagaccc gggctgttga   1920

gctgttcact gagtgttaca gcagcgatga agacttggca gaacagctgc tggtctattc   1980

ctgtgaagct tggggtggaa gcaactgtct ggagctggcg gtggaggcca cagaccagca   2040

tttcatcgcc cagcctgggg tccagaattt tctttctaag caatggtatg gagagatttc   2100

ccgagacacc aagaactgga agattatcct gtgtctgttt attataccct tggtgggctg   2160

tggctttgta tcatttagga agaaacctgt cgacaagcac aagaagctgc tttggtacta   2220

tgtggcgttc ttcacctccc ccttcgtggt cttctcctgg aatgtggtct tctacatcgc   2280

cttcctcctg ctgtttgcct acgtgctgct catggatttc cattcggtgc cacacccccc   2340

cgagctggtc ctgtactcgc tggtctttgt cctcttctgt gatgaagtga gacagtggta   2400

cgtaaatggg gtgaattatt ttactgacct gtggaatgtg atggacacgc tggggctttt   2460

ttacttcata gcaggaattg tatttcggct ccactcttct aataaaagct ctttgtattc   2520

tggacgagtc attttctgtc tggactacat tattttcact ctaagattga tccacatttt   2580

tactgtaagc agaaacttag gacccaagat tataatgctg cagaggatgc tgatcgatgt   2640

gttcttcttc ctgttcctct ttgcggtgtg gatggtggcc tttggcgtgg ccaggcaagg   2700

gatccttagg cagaatgagc agcgctggag gtggatattc cgttcggtca tctacgagcc   2760

ctacctggcc atgttcggcc aggtgcccag tgacgtggat ggtaccacgt atgactttgc   2820

ccactgcacc ttcactggga atgagtccaa gccactgtgt gtggagctgg atgagcacaa   2880

cctgccccgg ttccccgagt ggatcaccat ccccctggtg tgcatctaca tgttatccac   2940

caacatcctg ctggtcaacc tgctggtcgc catgtttggc tacacggtgg gcaccgtcca   3000

ggagaacaat gaccaggtct ggaagttcca gaggtacttc ctggtgcagg agtactgcag   3060

ccgcctcaat atccccttcc ccttcatcgt cttcgcttac ttctacatgg tggtgaagaa   3120

gtgcttcaag tgttgctgca aggagaaaaa catggagtct tctgtctgct gtttcaaaaa   3180

tgaagacaat gagactctgg catgggaggg tgtcatgaag gaaaactacc ttgtcaagat   3240

caacacaaaa gccaacgaca cctcagagga aatgaggcat cgatttagac aactggatac   3300

aaagcttaat gatctcaagg gtcttctgaa agagattgct aataaaatca aataaaactg   3360

tatgaactct aatggagaaa aatctaatta tagcaagatc atattaagga atgctgatga   3420

acaattttgc tatcgactac taaatgagag attttcagac ccctgggtac atggtggatg   3480

attttaaatc accctagtgt gctgagacct tgagaataaa gtgtgtgatt ggtttcatac   3540

ttgaagacgg atataaagga agaatatttc ctttatgtgt ttctccagaa tggtgcctgt   3600

ttctctctgt gtctcaatgc ctgggactgg aggttgatag tttaagtgtg ttcttaccgc   3660

ctcctttttc ctttaatctt atttttgatg aacacatata taggagaaca tctatcctat   3720

gaataagaac ctggtcatgc tttactcctg tattgttatt ttgttcattt ccaattgatt   3780

ctctactttt cccttttttg tattatgtga ctaattagtt ggcatattgt taaaagtctc   3840

tcaaattagg ccagattcta aaacatgctg cagcaagagg accccgctct cttcaggaaa   3900

agtgttttca tttctcagga tgcttcttac ctgtcagagg aggtgacaag gcagtctctt   3960

gctctcttgg actcaccagg ctcctattga aggaaccacc cccattccta aatatgtgaa   4020

aagtcgccca aaatgcaacc ttgaaaggca ctactgactt tgttcttatt ggatactcct   4080

cttattattt ttccattaaa aataatagct ggctattata gaaaatttag accatacaga   4140

gatgtagaaa gaacataaat tgtccccatt accttaaggt aatcactgct aacaatttct   4200

ggatggtttt tcaagtctat tttttttcta tgtatgtctc aattctcttt caaaatttta   4260

cagaatgtta tcatactaca tatatacttt ttatgtaagc tttttcactt agtattttat   4320

caaatatgtt tttattatat tcatagcctt cttaaacatt atatcaataa ttgcataata   4380

ggcaacctct agcgattacc ataattttgc tcattgaagg ctatctccag ttgatcattg   4440

ggatgagcat ctttgtgcat gaatcctatt gctgtatttg ggaaaatttt ccaaggttag   4500

attccaataa atatctattt attattaaat attaaaatat ctatttatta ttaaaaccat   4560

ttataaggct ttttcataaa tgtatagcaa ataggaatta ttaacttgag cataagatat   4620

gagatacatg aacctgaact attaaaataa aatattatat ttaaccctta gtttaagaag   4680

aagtcaatat gcttatttaa atattatgga tggtgggcag atcacttgag gtcaggagtt   4740

cgagaccagc ctggccaaca tggcaaaacc acatctctac taaaaataaa aaaattagct   4800

gggtgtggtg gtgcactcct gtaatcccag ctactcagaa ggctgaggta caagaattgc   4860

tggaacctgg gaggcggagg ttgcagtgaa ccaagattgc accactgcac tccagccggg   4920

gtgacagagt gagactccga ctgaaaataa ataaataaat aaataaataa ataaataaat   4980

attatggatg gtgaagggaa tggtatagaa ttggagagat tatcttactg aacacctgta   5040

gtcccagctt tctctggaag tggtcgtatt tgagcaggat gtgcacaagg caattgaaat   5100

gcccataatt agtttctcag ctttgaatac actataaact cactggctga aggaggaaat   5160

tttagaagga agctactaaa agatctaatt tgaaaaacta caaaagcatt aactaaaaaa   5220

gtttattttc cttttgtctg ggcagtagtg aaaataacta ctcacaacat tcactatgtt   5280

tgcaaggaat taacacaaat aaaagatgcc tttttactta aacaccaaga cagaaaactt   5340

gcccaatact gagaagcaac ttgcattaga gagggaactg ttaaatgttt tcaacccagt   5400

tcatctggtg gatgtttttg caggttactc tgagaatttt gcttatgaaa aatcattatt   5460

tttagtgtag ttcacaataa tgtattgaac atacttctaa tcaaaggtgc tatgtccttg   5520

tgtatggtac taaatgtgtc ctgtgtactt ttgcacaact gagaatcctg cagcttggtt   5580

taatgagtgt gttcatgaaa taaataatgg aggaattgtc a                       5621


<210>  2
<211>  1104
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ser Phe Arg Ala Ala Arg Leu Ser Met Arg Asn Arg Arg Asn Asp 
1               5                   10                  15      


Thr Leu Asp Ser Thr Arg Thr Leu Tyr Ser Ser Ala Ser Arg Ser Thr 
            20                  25                  30          


Asp Leu Ser Tyr Ser Glu Ser Asp Leu Val Asn Phe Ile Gln Ala Asn 
        35                  40                  45              


Phe Lys Lys Arg Glu Cys Val Phe Phe Thr Lys Asp Ser Lys Ala Thr 
    50                  55                  60                  


Glu Asn Val Cys Lys Cys Gly Tyr Ala Gln Ser Gln His Met Glu Gly 
65                  70                  75                  80  


Thr Gln Ile Asn Gln Ser Glu Lys Trp Asn Tyr Lys Lys His Thr Lys 
                85                  90                  95      


Glu Phe Pro Thr Asp Ala Phe Gly Asp Ile Gln Phe Glu Thr Leu Gly 
            100                 105                 110         


Lys Lys Gly Lys Tyr Ile Arg Leu Ser Cys Asp Thr Asp Ala Glu Ile 
        115                 120                 125             


Leu Tyr Glu Leu Leu Thr Gln His Trp His Leu Lys Thr Pro Asn Leu 
    130                 135                 140                 


Val Ile Ser Val Thr Gly Gly Ala Lys Asn Phe Ala Leu Lys Pro Arg 
145                 150                 155                 160 


Met Arg Lys Ile Phe Ser Arg Leu Ile Tyr Ile Ala Gln Ser Lys Gly 
                165                 170                 175     


Ala Trp Ile Leu Thr Gly Gly Thr His Tyr Gly Leu Met Lys Tyr Ile 
            180                 185                 190         


Gly Glu Val Val Arg Asp Asn Thr Ile Ser Arg Ser Ser Glu Glu Asn 
        195                 200                 205             


Ile Val Ala Ile Gly Ile Ala Ala Trp Gly Met Val Ser Asn Arg Asp 
    210                 215                 220                 


Thr Leu Ile Arg Asn Cys Asp Ala Glu Gly Tyr Phe Leu Ala Gln Tyr 
225                 230                 235                 240 


Leu Met Asp Asp Phe Thr Arg Asp Pro Leu Tyr Ile Leu Asp Asn Asn 
                245                 250                 255     


His Thr His Leu Leu Leu Val Asp Asn Gly Cys His Gly His Pro Thr 
            260                 265                 270         


Val Glu Ala Lys Leu Arg Asn Gln Leu Glu Lys Tyr Ile Ser Glu Arg 
        275                 280                 285             


Thr Ile Gln Asp Ser Asn Tyr Gly Gly Lys Ile Pro Ile Val Cys Phe 
    290                 295                 300                 


Ala Gln Gly Gly Gly Lys Glu Thr Leu Lys Ala Ile Asn Thr Ser Ile 
305                 310                 315                 320 


Lys Asn Lys Ile Pro Cys Val Val Val Glu Gly Ser Gly Gln Ile Ala 
                325                 330                 335     


Asp Val Ile Ala Ser Leu Val Glu Val Glu Asp Ala Leu Thr Ser Ser 
            340                 345                 350         


Ala Val Lys Glu Lys Leu Val Arg Phe Leu Pro Arg Thr Val Ser Arg 
        355                 360                 365             


Leu Pro Glu Glu Glu Thr Glu Ser Trp Ile Lys Trp Leu Lys Glu Ile 
    370                 375                 380                 


Leu Glu Cys Ser His Leu Leu Thr Val Ile Lys Met Glu Glu Ala Gly 
385                 390                 395                 400 


Asp Glu Ile Val Ser Asn Ala Ile Ser Tyr Ala Leu Tyr Lys Ala Phe 
                405                 410                 415     


Ser Thr Ser Glu Gln Asp Lys Asp Asn Trp Asn Gly Gln Leu Lys Leu 
            420                 425                 430         


Leu Leu Glu Trp Asn Gln Leu Asp Leu Ala Asn Asp Glu Ile Phe Thr 
        435                 440                 445             


Asn Asp Arg Arg Trp Glu Ser Ala Asp Leu Gln Glu Val Met Phe Thr 
    450                 455                 460                 


Ala Leu Ile Lys Asp Arg Pro Lys Phe Val Arg Leu Phe Leu Glu Asn 
465                 470                 475                 480 


Gly Leu Asn Leu Arg Lys Phe Leu Thr His Asp Val Leu Thr Glu Leu 
                485                 490                 495     


Phe Ser Asn His Phe Ser Thr Leu Val Tyr Arg Asn Leu Gln Ile Ala 
            500                 505                 510         


Lys Asn Ser Tyr Asn Asp Ala Leu Leu Thr Phe Val Trp Lys Leu Val 
        515                 520                 525             


Ala Asn Phe Arg Arg Gly Phe Arg Lys Glu Asp Arg Asn Gly Arg Asp 
    530                 535                 540                 


Glu Met Asp Ile Glu Leu His Asp Val Ser Pro Ile Thr Arg His Pro 
545                 550                 555                 560 


Leu Gln Ala Leu Phe Ile Trp Ala Ile Leu Gln Asn Lys Lys Glu Leu 
                565                 570                 575     


Ser Lys Val Ile Trp Glu Gln Thr Arg Gly Cys Thr Leu Ala Ala Leu 
            580                 585                 590         


Gly Ala Ser Lys Leu Leu Lys Thr Leu Ala Lys Val Lys Asn Asp Ile 
        595                 600                 605             


Asn Ala Ala Gly Glu Ser Glu Glu Leu Ala Asn Glu Tyr Glu Thr Arg 
    610                 615                 620                 


Ala Val Glu Leu Phe Thr Glu Cys Tyr Ser Ser Asp Glu Asp Leu Ala 
625                 630                 635                 640 


Glu Gln Leu Leu Val Tyr Ser Cys Glu Ala Trp Gly Gly Ser Asn Cys 
                645                 650                 655     


Leu Glu Leu Ala Val Glu Ala Thr Asp Gln His Phe Ile Ala Gln Pro 
            660                 665                 670         


Gly Val Gln Asn Phe Leu Ser Lys Gln Trp Tyr Gly Glu Ile Ser Arg 
        675                 680                 685             


Asp Thr Lys Asn Trp Lys Ile Ile Leu Cys Leu Phe Ile Ile Pro Leu 
    690                 695                 700                 


Val Gly Cys Gly Phe Val Ser Phe Arg Lys Lys Pro Val Asp Lys His 
705                 710                 715                 720 


Lys Lys Leu Leu Trp Tyr Tyr Val Ala Phe Phe Thr Ser Pro Phe Val 
                725                 730                 735     


Val Phe Ser Trp Asn Val Val Phe Tyr Ile Ala Phe Leu Leu Leu Phe 
            740                 745                 750         


Ala Tyr Val Leu Leu Met Asp Phe His Ser Val Pro His Pro Pro Glu 
        755                 760                 765             


Leu Val Leu Tyr Ser Leu Val Phe Val Leu Phe Cys Asp Glu Val Arg 
    770                 775                 780                 


Gln Trp Tyr Val Asn Gly Val Asn Tyr Phe Thr Asp Leu Trp Asn Val 
785                 790                 795                 800 


Met Asp Thr Leu Gly Leu Phe Tyr Phe Ile Ala Gly Ile Val Phe Arg 
                805                 810                 815     


Leu His Ser Ser Asn Lys Ser Ser Leu Tyr Ser Gly Arg Val Ile Phe 
            820                 825                 830         


Cys Leu Asp Tyr Ile Ile Phe Thr Leu Arg Leu Ile His Ile Phe Thr 
        835                 840                 845             


Val Ser Arg Asn Leu Gly Pro Lys Ile Ile Met Leu Gln Arg Met Leu 
    850                 855                 860                 


Ile Asp Val Phe Phe Phe Leu Phe Leu Phe Ala Val Trp Met Val Ala 
865                 870                 875                 880 


Phe Gly Val Ala Arg Gln Gly Ile Leu Arg Gln Asn Glu Gln Arg Trp 
                885                 890                 895     


Arg Trp Ile Phe Arg Ser Val Ile Tyr Glu Pro Tyr Leu Ala Met Phe 
            900                 905                 910         


Gly Gln Val Pro Ser Asp Val Asp Gly Thr Thr Tyr Asp Phe Ala His 
        915                 920                 925             


Cys Thr Phe Thr Gly Asn Glu Ser Lys Pro Leu Cys Val Glu Leu Asp 
    930                 935                 940                 


Glu His Asn Leu Pro Arg Phe Pro Glu Trp Ile Thr Ile Pro Leu Val 
945                 950                 955                 960 


Cys Ile Tyr Met Leu Ser Thr Asn Ile Leu Leu Val Asn Leu Leu Val 
                965                 970                 975     


Ala Met Phe Gly Tyr Thr Val Gly Thr Val Gln Glu Asn Asn Asp Gln 
            980                 985                 990         


Val Trp Lys Phe Gln Arg Tyr Phe  Leu Val Gln Glu Tyr  Cys Ser Arg 
        995                 1000                 1005             


Leu Asn  Ile Pro Phe Pro Phe  Ile Val Phe Ala Tyr  Phe Tyr Met 
    1010                 1015                 1020             


Val Val  Lys Lys Cys Phe Lys  Cys Cys Cys Lys Glu  Lys Asn Met 
    1025                 1030                 1035             


Glu Ser  Ser Val Cys Cys Phe  Lys Asn Glu Asp Asn  Glu Thr Leu 
    1040                 1045                 1050             


Ala Trp  Glu Gly Val Met Lys  Glu Asn Tyr Leu Val  Lys Ile Asn 
    1055                 1060                 1065             


Thr Lys  Ala Asn Asp Thr Ser  Glu Glu Met Arg His  Arg Phe Arg 
    1070                 1075                 1080             


Gln Leu  Asp Thr Lys Leu Asn  Asp Leu Lys Gly Leu  Leu Lys Glu 
    1085                 1090                 1095             


Ile Ala  Asn Lys Ile Lys 
    1100                 


