                         SEQUENCE LISTING

<110>  Novartis AG
 
<120>  Organic Compounds

<130>  PAT052777A

<160>  26    

<170>  PatentIn version 3.3

<210>  1
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  1
gctagcatgc gagaatatga tagattatat gaagaatata cc                        42


<210>  2
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  2
gcctccacca cctccgcctg gtttaatgct gttcatacgt ttgtc                     45


<210>  3
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  3
tactagtccg cctccaccac ctccgcctcc accacctccg cc                        42


<210>  4
<211>  1180
<212>  PRT
<213>  Artificial

<220>
<223>  PI3K kinase construct

<400>  4

Met Arg Glu Tyr Asp Arg Leu Tyr Glu Glu Tyr Thr Arg Thr Ser Gln 
1               5                   10                  15      


Glu Ile Gln Met Lys Arg Thr Ala Ile Glu Ala Phe Asn Glu Thr Ile 
            20                  25                  30          


Lys Ile Phe Glu Glu Gln Cys Gln Thr Gln Glu Arg Tyr Ser Lys Glu 
        35                  40                  45              


Tyr Ile Glu Lys Phe Lys Arg Glu Gly Asn Glu Lys Glu Ile Gln Arg 
    50                  55                  60                  


Ile Met His Asn Tyr Asp Lys Leu Lys Ser Arg Ile Ser Glu Ile Ile 
65                  70                  75                  80  


Asp Ser Arg Arg Arg Leu Glu Glu Asp Leu Lys Lys Gln Ala Ala Glu 
                85                  90                  95      


Tyr Arg Glu Ile Asp Lys Arg Met Asn Ser Ile Lys Pro Gly Gly Gly 
            100                 105                 110         


Gly Gly Gly Gly Gly Gly Gly Gly Gly Leu Val Glu Cys Leu Leu Pro 
        115                 120                 125             


Asn Gly Met Ile Val Thr Leu Glu Cys Leu Arg Glu Ala Thr Leu Ile 
    130                 135                 140                 


Thr Ile Lys His Glu Leu Phe Lys Glu Ala Arg Lys Tyr Pro Leu His 
145                 150                 155                 160 


Gln Leu Leu Gln Asp Glu Ser Ser Tyr Ile Phe Val Ser Val Thr Gln 
                165                 170                 175     


Glu Ala Glu Arg Glu Glu Phe Phe Asp Glu Thr Arg Arg Leu Cys Asp 
            180                 185                 190         


Leu Arg Leu Phe Gln Pro Phe Leu Lys Val Ile Glu Pro Val Gly Asn 
        195                 200                 205             


Arg Glu Glu Lys Ile Leu Asn Arg Glu Ile Gly Phe Ala Ile Gly Met 
    210                 215                 220                 


Pro Val Cys Glu Phe Asp Met Val Lys Asp Pro Glu Val Gln Asp Phe 
225                 230                 235                 240 


Arg Arg Asn Ile Leu Asn Val Cys Lys Glu Ala Val Asp Leu Arg Asp 
                245                 250                 255     


Leu Asn Ser Pro His Ser Arg Ala Met Tyr Val Tyr Pro Pro Asn Val 
            260                 265                 270         


Glu Ser Ser Pro Glu Leu Pro Lys His Ile Tyr Asn Lys Leu Asp Lys 
        275                 280                 285             


Gly Gln Ile Ile Val Val Ile Trp Val Ile Val Ser Pro Asn Asn Asp 
    290                 295                 300                 


Lys Gln Lys Tyr Thr Leu Lys Ile Asn His Asp Cys Val Pro Glu Gln 
305                 310                 315                 320 


Val Ile Ala Glu Ala Ile Arg Lys Lys Thr Arg Ser Met Leu Leu Ser 
                325                 330                 335     


Ser Glu Gln Leu Lys Leu Cys Val Leu Glu Tyr Gln Gly Lys Tyr Ile 
            340                 345                 350         


Leu Lys Val Cys Gly Cys Asp Glu Tyr Phe Leu Glu Lys Tyr Pro Leu 
        355                 360                 365             


Ser Gln Tyr Lys Tyr Ile Arg Ser Cys Ile Met Leu Gly Arg Met Pro 
    370                 375                 380                 


Asn Leu Met Leu Met Ala Lys Glu Ser Leu Tyr Ser Gln Leu Pro Met 
385                 390                 395                 400 


Asp Cys Phe Thr Met Pro Ser Tyr Ser Arg Arg Ile Ser Thr Ala Thr 
                405                 410                 415     


Pro Tyr Met Asn Gly Glu Thr Ser Thr Lys Ser Leu Trp Val Ile Asn 
            420                 425                 430         


Ser Ala Leu Arg Ile Lys Ile Leu Cys Ala Thr Tyr Val Asn Val Asn 
        435                 440                 445             


Ile Arg Asp Ile Asp Lys Ile Tyr Val Arg Thr Gly Ile Tyr His Gly 
    450                 455                 460                 


Gly Glu Pro Leu Cys Asp Asn Val Asn Thr Gln Arg Val Pro Cys Ser 
465                 470                 475                 480 


Asn Pro Arg Trp Asn Glu Trp Leu Asn Tyr Asp Ile Tyr Ile Pro Asp 
                485                 490                 495     


Leu Pro Arg Ala Ala Arg Leu Cys Leu Ser Ile Cys Ser Val Lys Gly 
            500                 505                 510         


Arg Lys Gly Ala Lys Glu Glu His Cys Pro Leu Ala Trp Gly Asn Ile 
        515                 520                 525             


Asn Leu Phe Asp Tyr Thr Asp Thr Leu Val Ser Gly Lys Met Ala Leu 
    530                 535                 540                 


Asn Leu Trp Pro Val Pro His Gly Leu Glu Asp Leu Leu Asn Pro Ile 
545                 550                 555                 560 


Gly Val Thr Gly Ser Asn Pro Asn Lys Glu Thr Pro Cys Leu Glu Leu 
                565                 570                 575     


Glu Phe Asp Trp Phe Ser Ser Val Val Lys Phe Pro Asp Met Ser Val 
            580                 585                 590         


Ile Glu Glu His Ala Asn Trp Ser Val Ser Arg Glu Ala Gly Phe Ser 
        595                 600                 605             


Tyr Ser His Ala Gly Leu Ser Asn Arg Leu Ala Arg Asp Asn Glu Leu 
    610                 615                 620                 


Arg Glu Asn Asp Lys Glu Gln Leu Lys Ala Ile Ser Thr Arg Asp Pro 
625                 630                 635                 640 


Leu Ser Glu Ile Thr Glu Gln Glu Lys Asp Phe Leu Trp Ser His Arg 
                645                 650                 655     


His Tyr Cys Val Thr Ile Pro Glu Ile Leu Pro Lys Leu Leu Leu Ser 
            660                 665                 670         


Val Lys Trp Asn Ser Arg Asp Glu Val Ala Gln Met Tyr Cys Leu Val 
        675                 680                 685             


Lys Asp Trp Pro Pro Ile Lys Pro Glu Gln Ala Met Glu Leu Leu Asp 
    690                 695                 700                 


Cys Asn Tyr Pro Asp Pro Met Val Arg Gly Phe Ala Val Arg Cys Leu 
705                 710                 715                 720 


Glu Lys Tyr Leu Thr Asp Asp Lys Leu Ser Gln Tyr Leu Ile Gln Leu 
                725                 730                 735     


Val Gln Val Leu Lys Tyr Glu Gln Tyr Leu Asp Asn Leu Leu Val Arg 
            740                 745                 750         


Phe Leu Leu Lys Lys Ala Leu Thr Asn Gln Arg Ile Gly His Phe Phe 
        755                 760                 765             


Phe Trp His Leu Lys Ser Glu Met His Asn Lys Thr Val Ser Gln Arg 
    770                 775                 780                 


Phe Gly Leu Leu Leu Glu Ser Tyr Cys Arg Ala Cys Gly Met Tyr Leu 
785                 790                 795                 800 


Lys His Leu Asn Arg Gln Val Glu Ala Met Glu Lys Leu Ile Asn Leu 
                805                 810                 815     


Thr Asp Ile Leu Lys Gln Glu Lys Lys Asp Glu Thr Gln Lys Val Gln 
            820                 825                 830         


Met Lys Phe Leu Val Glu Gln Met Arg Arg Pro Asp Phe Met Asp Ala 
        835                 840                 845             


Leu Gln Gly Phe Leu Ser Pro Leu Asn Pro Ala His Gln Leu Gly Asn 
    850                 855                 860                 


Leu Arg Leu Glu Glu Cys Arg Ile Met Ser Ser Ala Lys Arg Pro Leu 
865                 870                 875                 880 


Trp Leu Asn Trp Glu Asn Pro Asp Ile Met Ser Glu Leu Leu Phe Gln 
                885                 890                 895     


Asn Asn Glu Ile Ile Phe Lys Asn Gly Asp Asp Leu Arg Gln Asp Met 
            900                 905                 910         


Leu Thr Leu Gln Ile Ile Arg Ile Met Glu Asn Ile Trp Gln Asn Gln 
        915                 920                 925             


Gly Leu Asp Leu Arg Met Leu Pro Tyr Gly Cys Leu Ser Ile Gly Asp 
    930                 935                 940                 


Cys Val Gly Leu Ile Glu Val Val Arg Asn Ser His Thr Ile Met Gln 
945                 950                 955                 960 


Ile Gln Cys Lys Gly Gly Leu Lys Gly Ala Leu Gln Phe Asn Ser His 
                965                 970                 975     


Thr Leu His Gln Trp Leu Lys Asp Lys Asn Lys Gly Glu Ile Tyr Asp 
            980                 985                 990         


Ala Ala Ile Asp Leu Phe Thr Arg  Ser Cys Ala Gly Tyr  Cys Val Ala 
        995                 1000                 1005             


Thr Phe  Ile Leu Gly Ile Gly  Asp Arg His Asn Ser  Asn Ile Met 
    1010                 1015                 1020             


Val Lys  Asp Asp Gly Gln Leu  Phe His Ile Asp Phe  Gly His Phe 
    1025                 1030                 1035             


Leu Asp  His Lys Lys Lys Lys  Phe Gly Tyr Lys Arg  Glu Arg Val 
    1040                 1045                 1050             


Pro Phe  Val Leu Thr Gln Asp  Phe Leu Ile Val Ile  Ser Lys Gly 
    1055                 1060                 1065             


Ala Gln  Glu Cys Thr Lys Thr  Arg Glu Phe Glu Arg  Phe Gln Glu 
    1070                 1075                 1080             


Met Cys  Tyr Lys Ala Tyr Leu  Ala Ile Arg Gln His  Ala Asn Leu 
    1085                 1090                 1095             


Phe Ile  Asn Leu Phe Ser Met  Met Leu Gly Ser Gly  Met Pro Glu 
    1100                 1105                 1110             


Leu Gln  Ser Phe Asp Asp Ile  Ala Tyr Ile Arg Lys  Thr Leu Ala 
    1115                 1120                 1125             


Leu Asp  Lys Thr Glu Gln Glu  Ala Leu Glu Tyr Phe  Met Lys Gln 
    1130                 1135                 1140             


Met Asn  Asp Ala His His Gly  Gly Trp Thr Thr Lys  Met Asp Trp 
    1145                 1150                 1155             


Ile Phe  His Thr Ile Lys Gln  His Ala Leu Asn Glu  Leu Gly Gly 
    1160                 1165                 1170             


Ala His  His His His His His  
    1175                 1180 


<210>  5
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  5
cgagaatatg atagattata tgaagaat                                        28


<210>  6
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  6
tggtttaatg ctgttcatac gtttgtcaat                                      30


<210>  7
<211>  76
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  7
gggacaagtt tgtacaaaaa agcaggctac gaaggagata tacatatgcg agaatatgat     60

agattatatg aagaat                                                     76


<210>  8
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  8
actgaagcat cctcctcctc ctcctcctgg tttaatgctg ttcatacgtt tgtc           54


<210>  9
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  9
attaaaccag gaggaggagg aggaggatgc ttcagtttca taatgcctcc tgct           54


<210>  10
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  10
agctccgtga tggtgatggt gatgtgctcc agatctgtag tctttccgaa ctgtgtg        57


<210>  11
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  11
gggaccactt tgtacaagaa agctgggttt aagctccgtg atggtgatgg tgatgtgctc     60

c                                                                     61


<210>  12
<211>  1194
<212>  PRT
<213>  Artificial

<220>
<223>  PI3K kinase construct

<400>  12

Met Arg Glu Tyr Asp Arg Leu Tyr Glu Glu Tyr Thr Arg Thr Ser Gln 
1               5                   10                  15      


Glu Ile Gln Met Lys Arg Thr Ala Ile Glu Ala Phe Asn Glu Thr Ile 
            20                  25                  30          


Lys Ile Phe Glu Glu Gln Cys Gln Thr Gln Glu Arg Tyr Ser Lys Glu 
        35                  40                  45              


Tyr Ile Glu Lys Phe Lys Arg Glu Gly Lys Glu Lys Glu Ile Gln Arg 
    50                  55                  60                  


Ile Met His Asn Tyr Asp Lys Leu Lys Ser Arg Ile Ser Glu Ile Ile 
65                  70                  75                  80  


Asp Ser Arg Arg Arg Leu Glu Glu Asp Leu Lys Lys Gln Ala Ala Glu 
                85                  90                  95      


Tyr Arg Glu Ile Asp Lys Arg Met Asn Ser Ile Lys Pro Gly Gly Gly 
            100                 105                 110         


Gly Gly Gly Cys Phe Ser Phe Ile Met Pro Pro Ala Met Ala Asp Ile 
        115                 120                 125             


Leu Asp Ile Trp Ala Val Asp Ser Gln Ile Ala Ser Asp Gly Ser Ile 
    130                 135                 140                 


Pro Val Asp Phe Leu Leu Pro Thr Gly Ile Tyr Ile Gln Leu Glu Val 
145                 150                 155                 160 


Pro Arg Glu Ala Thr Ile Ser Tyr Ile Lys Gln Met Leu Trp Lys Gln 
                165                 170                 175     


Val His Asn Tyr Pro Met Phe Asn Leu Leu Met Asp Ile Asp Ser Tyr 
            180                 185                 190         


Met Phe Ala Cys Val Asn Gln Thr Ala Val Tyr Glu Glu Leu Glu Asp 
        195                 200                 205             


Glu Thr Arg Arg Leu Cys Asp Val Arg Pro Phe Leu Pro Val Leu Lys 
    210                 215                 220                 


Leu Val Thr Arg Ser Cys Asp Pro Gly Glu Lys Leu Asp Ser Lys Ile 
225                 230                 235                 240 


Gly Val Leu Ile Gly Lys Gly Leu His Glu Phe Asp Ser Leu Lys Asp 
                245                 250                 255     


Pro Glu Val Asn Glu Phe Arg Arg Lys Met Arg Lys Phe Ser Glu Glu 
            260                 265                 270         


Lys Ile Leu Ser Leu Val Gly Leu Ser Trp Met Asp Trp Leu Lys Gln 
        275                 280                 285             


Thr Tyr Pro Pro Glu His Glu Pro Ser Ile Pro Glu Asn Leu Glu Asp 
    290                 295                 300                 


Lys Leu Tyr Gly Gly Lys Leu Ile Val Ala Val His Phe Glu Asn Cys 
305                 310                 315                 320 


Gln Asp Val Phe Ser Phe Gln Val Ser Pro Asn Met Asn Pro Ile Lys 
                325                 330                 335     


Val Asn Glu Leu Ala Ile Gln Lys Arg Leu Thr Ile His Gly Lys Glu 
            340                 345                 350         


Asp Glu Val Ser Pro Tyr Asp Tyr Val Leu Gln Val Ser Gly Arg Val 
        355                 360                 365             


Glu Tyr Val Phe Gly Asp His Pro Leu Ile Gln Phe Gln Tyr Ile Arg 
    370                 375                 380                 


Asn Cys Val Met Asn Arg Ala Leu Pro His Phe Ile Leu Val Glu Cys 
385                 390                 395                 400 


Cys Lys Ile Lys Lys Met Tyr Glu Gln Glu Met Ile Ala Ile Glu Ala 
                405                 410                 415     


Ala Ile Asn Arg Asn Ser Ser Asn Leu Pro Leu Pro Leu Pro Pro Lys 
            420                 425                 430         


Lys Thr Arg Ile Ile Ser His Val Trp Glu Asn Asn Asn Pro Phe Gln 
        435                 440                 445             


Ile Val Leu Val Lys Gly Asn Lys Leu Asn Thr Glu Glu Thr Val Lys 
    450                 455                 460                 


Val His Val Arg Ala Gly Leu Phe His Gly Thr Glu Leu Leu Cys Lys 
465                 470                 475                 480 


Thr Ile Val Ser Ser Glu Val Ser Gly Lys Asn Asp His Ile Trp Asn 
                485                 490                 495     


Glu Pro Leu Glu Phe Asp Ile Asn Ile Cys Asp Leu Pro Arg Met Ala 
            500                 505                 510         


Arg Leu Cys Phe Ala Val Tyr Ala Val Leu Asp Lys Val Lys Thr Lys 
        515                 520                 525             


Lys Ser Thr Lys Thr Ile Asn Pro Ser Lys Tyr Gln Thr Ile Arg Lys 
    530                 535                 540                 


Ala Gly Lys Val His Tyr Pro Val Ala Trp Val Asn Thr Met Val Phe 
545                 550                 555                 560 


Asp Phe Lys Gly Gln Leu Arg Thr Gly Asp Ile Ile Leu His Ser Trp 
                565                 570                 575     


Ser Ser Phe Pro Asp Glu Leu Glu Glu Met Leu Asn Pro Met Gly Thr 
            580                 585                 590         


Val Gln Thr Asn Pro Tyr Thr Glu Asn Ala Thr Ala Leu His Val Lys 
        595                 600                 605             


Phe Pro Glu Asn Lys Lys Gln Pro Tyr Tyr Tyr Pro Pro Phe Asp Lys 
    610                 615                 620                 


Ile Ile Glu Lys Ala Ala Glu Ile Ala Ser Ser Asp Ser Ala Asn Val 
625                 630                 635                 640 


Ser Ser Arg Gly Gly Lys Lys Phe Leu Pro Val Leu Lys Glu Ile Leu 
                645                 650                 655     


Asp Arg Asp Pro Leu Ser Gln Leu Cys Glu Asn Glu Met Asp Leu Ile 
            660                 665                 670         


Trp Thr Leu Arg Gln Asp Cys Arg Glu Ile Phe Pro Gln Ser Leu Pro 
        675                 680                 685             


Lys Leu Leu Leu Ser Ile Lys Trp Asn Lys Leu Glu Asp Val Ala Gln 
    690                 695                 700                 


Leu Gln Ala Leu Leu Gln Ile Trp Pro Lys Leu Pro Pro Arg Glu Ala 
705                 710                 715                 720 


Leu Glu Leu Leu Asp Phe Asn Tyr Pro Asp Gln Tyr Val Arg Glu Tyr 
                725                 730                 735     


Ala Val Gly Cys Leu Arg Gln Met Ser Asp Glu Glu Leu Ser Gln Tyr 
            740                 745                 750         


Leu Leu Gln Leu Val Gln Val Leu Lys Tyr Glu Pro Phe Leu Asp Cys 
        755                 760                 765             


Ala Leu Ser Arg Phe Leu Leu Glu Arg Ala Leu Gly Asn Arg Arg Ile 
    770                 775                 780                 


Gly Gln Phe Leu Phe Trp His Leu Arg Ser Glu Val His Ile Pro Ala 
785                 790                 795                 800 


Val Ser Val Gln Phe Gly Val Ile Leu Glu Ala Tyr Cys Arg Gly Ser 
                805                 810                 815     


Val Gly His Met Lys Val Leu Ser Lys Gln Val Glu Ala Leu Asn Lys 
            820                 825                 830         


Leu Lys Thr Leu Asn Ser Leu Ile Lys Leu Asn Ala Val Lys Leu Asn 
        835                 840                 845             


Arg Ala Lys Gly Lys Glu Ala Met His Thr Cys Leu Lys Gln Ser Ala 
    850                 855                 860                 


Tyr Arg Glu Ala Leu Ser Asp Leu Gln Ser Pro Leu Asn Pro Cys Val 
865                 870                 875                 880 


Ile Leu Ser Glu Leu Tyr Val Glu Lys Cys Lys Tyr Met Asp Ser Lys 
                885                 890                 895     


Met Lys Pro Leu Trp Leu Val Tyr Asn Asn Lys Val Phe Gly Glu Asp 
            900                 905                 910         


Ser Val Gly Val Ile Phe Lys Asn Gly Asp Asp Leu Arg Gln Asp Met 
        915                 920                 925             


Leu Thr Leu Gln Met Leu Arg Leu Met Asp Leu Leu Trp Lys Glu Ala 
    930                 935                 940                 


Gly Leu Asp Leu Arg Met Leu Pro Tyr Gly Cys Leu Ala Thr Gly Asp 
945                 950                 955                 960 


Arg Ser Gly Leu Ile Glu Val Val Ser Thr Ser Glu Thr Ile Ala Asp 
                965                 970                 975     


Ile Gln Leu Asn Ser Ser Asn Val Ala Ala Ala Ala Ala Phe Asn Lys 
            980                 985                 990         


Asp Ala Leu Leu Asn Trp Leu Lys  Glu Tyr Asn Ser Gly  Asp Asp Leu 
        995                 1000                 1005             


Asp Arg  Ala Ile Glu Glu Phe  Thr Leu Ser Cys Ala  Gly Tyr Cys 
    1010                 1015                 1020             


Val Ala  Ser Tyr Val Leu Gly  Ile Gly Asp Arg His  Ser Asp Asn 
    1025                 1030                 1035             


Ile Met  Val Lys Lys Thr Gly  Gln Leu Phe His Ile  Asp Phe Gly 
    1040                 1045                 1050             


His Ile  Leu Gly Asn Phe Lys  Ser Lys Phe Gly Ile  Lys Arg Glu 
    1055                 1060                 1065             


Arg Val  Pro Phe Ile Leu Thr  Tyr Asp Phe Ile His  Val Ile Gln 
    1070                 1075                 1080             


Gln Gly  Lys Thr Gly Asn Thr  Glu Lys Phe Gly Arg  Phe Arg Gln 
    1085                 1090                 1095             


Cys Cys  Glu Asp Ala Tyr Leu  Ile Leu Arg Arg His  Gly Asn Leu 
    1100                 1105                 1110             


Phe Ile  Thr Leu Phe Ala Leu  Met Leu Thr Ala Gly  Leu Pro Glu 
    1115                 1120                 1125             


Leu Thr  Ser Val Lys Asp Ile  Gln Tyr Leu Lys Asp  Ser Leu Ala 
    1130                 1135                 1140             


Leu Gly  Lys Ser Glu Glu Glu  Ala Leu Lys Gln Phe  Lys Gln Lys 
    1145                 1150                 1155             


Phe Asp  Glu Ala Leu Arg Glu  Ser Trp Thr Thr Lys  Val Asn Trp 
    1160                 1165                 1170             


Met Ala  His Thr Val Arg Lys  Asp Tyr Arg Ser Gly  Ala His His 
    1175                 1180                 1185             


His His  His His Gly Ala 
    1190                 


<210>  13
<211>  966
<212>  PRT
<213>  Artificial

<220>
<223>  PI3K kinase construct

<400>  13

Met Ser Glu Glu Ser Gln Ala Phe Gln Arg Gln Leu Thr Ala Leu Ile 
1               5                   10                  15      


Gly Tyr Asp Val Thr Asp Val Ser Asn Val His Asp Asp Glu Leu Glu 
            20                  25                  30          


Phe Thr Arg Arg Gly Leu Val Thr Pro Arg Met Ala Glu Val Ala Ser 
        35                  40                  45              


Arg Asp Pro Lys Leu Tyr Ala Met His Pro Trp Val Thr Ser Lys Pro 
    50                  55                  60                  


Leu Pro Glu Tyr Leu Trp Lys Lys Ile Ala Asn Asn Cys Ile Phe Ile 
65                  70                  75                  80  


Val Ile His Arg Ser Thr Thr Ser Gln Thr Ile Lys Val Ser Pro Asp 
                85                  90                  95      


Asp Thr Pro Gly Ala Ile Leu Gln Ser Phe Phe Thr Lys Met Ala Lys 
            100                 105                 110         


Lys Lys Ser Leu Met Asp Ile Pro Glu Ser Gln Ser Glu Gln Asp Phe 
        115                 120                 125             


Val Leu Arg Val Cys Gly Arg Asp Glu Tyr Leu Val Gly Glu Thr Pro 
    130                 135                 140                 


Ile Lys Asn Phe Gln Trp Val Arg His Cys Leu Lys Asn Gly Glu Glu 
145                 150                 155                 160 


Ile His Val Val Leu Asp Thr Pro Pro Asp Pro Ala Leu Asp Glu Val 
                165                 170                 175     


Arg Lys Glu Glu Trp Pro Leu Val Asp Asp Cys Thr Gly Val Thr Gly 
            180                 185                 190         


Tyr His Glu Gln Leu Thr Ile His Gly Lys Asp His Glu Ser Val Phe 
        195                 200                 205             


Thr Val Ser Leu Trp Asp Cys Asp Arg Lys Phe Arg Val Lys Ile Arg 
    210                 215                 220                 


Gly Ile Asp Ile Pro Val Leu Pro Arg Asn Thr Asp Leu Thr Val Phe 
225                 230                 235                 240 


Val Glu Ala Asn Ile Gln His Gly Gln Gln Val Leu Cys Gln Arg Arg 
                245                 250                 255     


Thr Ser Pro Lys Pro Phe Thr Glu Glu Val Leu Trp Asn Val Trp Leu 
            260                 265                 270         


Glu Phe Ser Ile Lys Ile Lys Asp Leu Pro Lys Gly Ala Leu Leu Asn 
        275                 280                 285             


Leu Gln Ile Tyr Cys Gly Lys Ala Pro Ala Leu Ser Ser Lys Ala Ser 
    290                 295                 300                 


Ala Glu Ser Pro Ser Ser Glu Ser Lys Gly Lys Val Arg Leu Leu Tyr 
305                 310                 315                 320 


Tyr Val Asn Leu Leu Leu Ile Asp His Arg Phe Leu Leu Arg Arg Gly 
                325                 330                 335     


Glu Tyr Val Leu His Met Trp Gln Ile Ser Gly Lys Gly Glu Asp Gln 
            340                 345                 350         


Gly Ser Phe Asn Ala Asp Lys Leu Thr Ser Ala Thr Asn Pro Asp Lys 
        355                 360                 365             


Glu Asn Ser Met Ser Ile Ser Ile Leu Leu Asp Asn Tyr Cys His Pro 
    370                 375                 380                 


Ile Ala Leu Pro Lys His Gln Pro Thr Pro Asp Pro Glu Gly Asp Arg 
385                 390                 395                 400 


Val Arg Ala Glu Met Pro Asn Gln Leu Arg Lys Gln Leu Glu Ala Ile 
                405                 410                 415     


Ile Ala Thr Asp Pro Leu Asn Pro Leu Thr Ala Glu Asp Lys Glu Leu 
            420                 425                 430         


Leu Trp His Phe Arg Tyr Glu Ser Leu Lys His Pro Lys Ala Tyr Pro 
        435                 440                 445             


Lys Leu Phe Ser Ser Val Lys Trp Gly Gln Gln Glu Ile Val Ala Lys 
    450                 455                 460                 


Thr Tyr Gln Leu Leu Ala Arg Arg Glu Val Trp Asp Gln Ser Ala Leu 
465                 470                 475                 480 


Asp Val Gly Leu Thr Met Gln Leu Leu Asp Cys Asn Phe Ser Asp Glu 
                485                 490                 495     


Asn Val Arg Ala Ile Ala Val Gln Lys Leu Glu Ser Leu Glu Asp Asp 
            500                 505                 510         


Asp Val Leu His Tyr Leu Leu Gln Leu Val Gln Ala Val Lys Phe Glu 
        515                 520                 525             


Pro Tyr His Asp Ser Ala Leu Ala Arg Phe Leu Leu Lys Arg Gly Leu 
    530                 535                 540                 


Arg Asn Lys Arg Ile Gly His Phe Leu Phe Trp Phe Leu Arg Ser Glu 
545                 550                 555                 560 


Ile Ala Gln Ser Arg His Tyr Gln Gln Arg Phe Ala Val Ile Leu Glu 
                565                 570                 575     


Ala Tyr Leu Arg Gly Cys Gly Thr Ala Met Leu His Asp Phe Thr Gln 
            580                 585                 590         


Gln Val Gln Val Ile Glu Met Leu Gln Lys Val Thr Leu Asp Ile Lys 
        595                 600                 605             


Ser Leu Ser Ala Glu Lys Tyr Asp Val Ser Ser Gln Val Ile Ser Gln 
    610                 615                 620                 


Leu Lys Gln Lys Leu Glu Asn Leu Gln Asn Ser Gln Leu Pro Glu Ser 
625                 630                 635                 640 


Phe Arg Val Pro Tyr Asp Pro Gly Leu Lys Ala Gly Ala Leu Ala Ile 
                645                 650                 655     


Glu Lys Cys Lys Val Met Ala Ser Lys Lys Lys Pro Leu Trp Leu Glu 
            660                 665                 670         


Phe Lys Cys Ala Asp Pro Thr Ala Leu Ser Asn Glu Thr Ile Gly Ile 
        675                 680                 685             


Ile Phe Lys His Gly Asp Asp Leu Arg Gln Asp Met Leu Ile Leu Gln 
    690                 695                 700                 


Ile Leu Arg Ile Met Glu Ser Ile Trp Glu Thr Glu Ser Leu Asp Leu 
705                 710                 715                 720 


Cys Leu Leu Pro Tyr Gly Cys Ile Ser Thr Gly Asp Lys Ile Gly Met 
                725                 730                 735     


Ile Glu Ile Val Lys Asp Ala Thr Thr Ile Ala Lys Ile Gln Gln Ser 
            740                 745                 750         


Thr Val Gly Asn Thr Gly Ala Phe Lys Asp Glu Val Leu Asn His Trp 
        755                 760                 765             


Leu Lys Glu Lys Ser Pro Thr Glu Glu Lys Phe Gln Ala Ala Val Glu 
    770                 775                 780                 


Arg Phe Val Tyr Ser Cys Ala Gly Tyr Cys Val Ala Thr Phe Val Leu 
785                 790                 795                 800 


Gly Ile Gly Asp Arg His Asn Asp Asn Ile Met Ile Thr Glu Thr Gly 
                805                 810                 815     


Asn Leu Phe His Ile Asp Phe Gly His Ile Leu Gly Asn Tyr Lys Ser 
            820                 825                 830         


Phe Leu Gly Ile Asn Lys Glu Arg Val Pro Phe Val Leu Thr Pro Asp 
        835                 840                 845             


Phe Leu Phe Val Met Gly Thr Ser Gly Lys Lys Thr Ser Pro His Phe 
    850                 855                 860                 


Gln Lys Phe Gln Asp Ile Cys Val Lys Ala Tyr Leu Ala Leu Arg His 
865                 870                 875                 880 


His Thr Asn Leu Leu Ile Ile Leu Phe Ser Met Met Leu Met Thr Gly 
                885                 890                 895     


Met Pro Gln Leu Thr Ser Lys Glu Asp Ile Glu Tyr Ile Arg Asp Ala 
            900                 905                 910         


Leu Thr Val Gly Lys Asn Glu Glu Asp Ala Lys Lys Tyr Phe Leu Asp 
        915                 920                 925             


Gln Ile Glu Val Cys Arg Asp Lys Gly Trp Thr Val Gln Phe Asn Trp 
    930                 935                 940                 


Phe Leu His Leu Val Leu Gly Ile Lys Gln Gly Glu Lys His Ser Ala 
945                 950                 955                 960 


His His His His His His 
                965     


<210>  14
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  14
tcctcctcct cctcctcctg gtttaatgct gttcatacgt ttgtc                     45


<210>  15
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  15
atgccccctg gggtggactg ccccat                                          26


<210>  16
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  16
ctactgcctg ttgtctttgg acacgt                                          26


<210>  17
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  17
attaaaccag gaggaggagg aggaggaccc cctggggtgg actgccccat gga            53


<210>  18
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  18
agctccgtga tggtgatggt gatgtgctcc ctgcctgttg tctttggaca cgttgt         56


<210>  19
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  19
gggaccactt tgtacaagaa agctgggttt aagctccgtg atggtgatgg tgagtgctcc     60


<210>  20
<211>  1169
<212>  PRT
<213>  Artificial

<220>
<223>  PI3K kinase construct

<400>  20

Met Arg Glu Tyr Asp Arg Leu Tyr Glu Glu Tyr Thr Arg Thr Ser Gln 
1               5                   10                  15      


Glu Ile Gln Met Lys Arg Thr Ala Ile Glu Ala Phe Asn Glu Thr Ile 
            20                  25                  30          


Lys Ile Phe Glu Glu Gln Cys Gln Thr Gln Glu Arg Tyr Ser Lys Glu 
        35                  40                  45              


Tyr Ile Glu Lys Phe Lys Arg Glu Gly Asn Glu Lys Glu Ile Gln Arg 
    50                  55                  60                  


Ile Met His Asn Tyr Asp Lys Leu Lys Ser Arg Ile Ser Glu Ile Ile 
65                  70                  75                  80  


Asp Ser Arg Arg Arg Leu Glu Glu Asp Leu Lys Lys Gln Ala Ala Glu 
                85                  90                  95      


Tyr Arg Glu Ile Asp Lys Arg Met Asn Ser Ile Lys Pro Gly Gly Gly 
            100                 105                 110         


Gly Gly Gly Pro Pro Gly Val Asp Cys Pro Met Glu Phe Trp Thr Lys 
        115                 120                 125             


Glu Glu Asn Gln Ser Val Val Val Asp Phe Leu Leu Pro Thr Gly Val 
    130                 135                 140                 


Tyr Leu Asn Phe Pro Val Ser Arg Asn Ala Asn Leu Ser Thr Ile Lys 
145                 150                 155                 160 


Gln Leu Leu Trp His Arg Ala Gln Tyr Glu Pro Leu Phe His Met Leu 
                165                 170                 175     


Ser Gly Pro Glu Ala Tyr Val Phe Thr Cys Ile Asn Gln Thr Ala Glu 
            180                 185                 190         


Gln Gln Glu Leu Glu Asp Glu Gln Arg Arg Leu Cys Asp Val Gln Pro 
        195                 200                 205             


Phe Leu Pro Val Leu Arg Leu Val Ala Arg Glu Gly Asp Arg Val Lys 
    210                 215                 220                 


Lys Leu Ile Asn Ser Gln Ile Ser Leu Leu Ile Gly Lys Gly Leu His 
225                 230                 235                 240 


Glu Phe Asp Ser Leu Cys Asp Pro Glu Val Asn Asp Phe Arg Ala Lys 
                245                 250                 255     


Met Cys Gln Phe Cys Glu Glu Ala Ala Ala Arg Arg Gln Gln Leu Gly 
            260                 265                 270         


Trp Glu Ala Trp Leu Gln Tyr Ser Phe Pro Leu Gln Leu Glu Pro Ser 
        275                 280                 285             


Ala Gln Thr Trp Gly Pro Gly Thr Leu Arg Leu Pro Asn Arg Ala Leu 
    290                 295                 300                 


Leu Val Asn Val Lys Phe Glu Gly Ser Glu Glu Ser Phe Thr Phe Gln 
305                 310                 315                 320 


Val Ser Thr Lys Asp Val Pro Leu Ala Leu Met Ala Cys Ala Leu Arg 
                325                 330                 335     


Lys Lys Ala Thr Val Phe Arg Gln Pro Leu Val Glu Gln Pro Glu Asp 
            340                 345                 350         


Tyr Thr Leu Gln Val Asn Gly Arg His Glu Tyr Leu Tyr Gly Ser Tyr 
        355                 360                 365             


Pro Leu Cys Gln Phe Gln Tyr Ile Cys Ser Cys Leu His Ser Gly Leu 
    370                 375                 380                 


Thr Pro His Leu Thr Met Val His Ser Ser Ser Ile Leu Ala Met Arg 
385                 390                 395                 400 


Asp Glu Gln Ser Asn Pro Ala Pro Gln Val Gln Lys Pro Arg Ala Lys 
                405                 410                 415     


Pro Pro Pro Ile Pro Ala Lys Lys Pro Ser Ser Val Ser Leu Trp Ser 
            420                 425                 430         


Leu Glu Gln Pro Phe Arg Ile Glu Leu Ile Gln Gly Ser Lys Val Asn 
        435                 440                 445             


Ala Asp Glu Arg Met Lys Leu Val Val Gln Ala Gly Leu Phe His Gly 
    450                 455                 460                 


Asn Glu Met Leu Cys Lys Thr Val Ser Ser Ser Glu Val Ser Val Cys 
465                 470                 475                 480 


Ser Glu Pro Val Trp Lys Gln Arg Leu Glu Phe Asp Ile Asn Ile Cys 
                485                 490                 495     


Asp Leu Pro Arg Met Ala Arg Leu Cys Phe Ala Leu Tyr Ala Val Ile 
            500                 505                 510         


Glu Lys Ala Lys Lys Ala Arg Ser Thr Lys Lys Lys Ser Lys Lys Ala 
        515                 520                 525             


Asp Cys Pro Ile Ala Trp Ala Asn Leu Met Leu Phe Asp Tyr Lys Asp 
    530                 535                 540                 


Gln Leu Lys Thr Gly Glu Arg Cys Leu Tyr Met Trp Pro Ser Val Pro 
545                 550                 555                 560 


Asp Glu Lys Gly Glu Leu Leu Asn Pro Thr Gly Thr Val Arg Ser Asn 
                565                 570                 575     


Pro Asn Thr Asp Ser Ala Ala Ala Leu Leu Ile Cys Leu Pro Glu Val 
            580                 585                 590         


Ala Pro His Pro Val Tyr Tyr Pro Ala Leu Glu Lys Ile Leu Glu Leu 
        595                 600                 605             


Gly Arg His Ser Glu Cys Val His Val Thr Glu Glu Glu Gln Leu Gln 
    610                 615                 620                 


Leu Arg Glu Ile Leu Glu Arg Arg Gly Ser Gly Glu Leu Tyr Glu His 
625                 630                 635                 640 


Glu Lys Asp Leu Val Trp Lys Leu Arg His Glu Val Gln Glu His Phe 
                645                 650                 655     


Pro Glu Ala Leu Ala Arg Leu Leu Leu Val Thr Lys Trp Asn Lys His 
            660                 665                 670         


Glu Asp Val Ala Gln Met Leu Tyr Leu Leu Cys Ser Trp Pro Glu Leu 
        675                 680                 685             


Pro Val Leu Ser Ala Leu Glu Leu Leu Asp Phe Ser Phe Pro Asp Cys 
    690                 695                 700                 


His Val Gly Ser Phe Ala Ile Lys Ser Leu Arg Lys Leu Thr Asp Asp 
705                 710                 715                 720 


Glu Leu Phe Gln Tyr Leu Leu Gln Leu Val Gln Val Leu Lys Tyr Glu 
                725                 730                 735     


Ser Tyr Leu Asp Cys Glu Leu Thr Lys Phe Leu Leu Asp Arg Ala Leu 
            740                 745                 750         


Ala Asn Arg Lys Ile Gly His Phe Leu Phe Trp His Leu Arg Ser Glu 
        755                 760                 765             


Met His Val Pro Ser Val Ala Leu Arg Phe Gly Leu Ile Leu Glu Ala 
    770                 775                 780                 


Tyr Cys Arg Gly Ser Thr His His Met Lys Val Leu Met Lys Gln Gly 
785                 790                 795                 800 


Glu Ala Leu Ser Lys Leu Lys Ala Leu Asn Asp Phe Val Lys Leu Ser 
                805                 810                 815     


Ser Gln Lys Thr Pro Lys Pro Gln Thr Lys Glu Leu Met His Leu Cys 
            820                 825                 830         


Met Arg Gln Glu Ala Tyr Leu Glu Ala Leu Ser His Leu Gln Ser Pro 
        835                 840                 845             


Leu Asp Pro Ser Thr Leu Leu Ala Glu Val Cys Val Glu Gln Cys Thr 
    850                 855                 860                 


Phe Met Asp Ser Lys Met Lys Pro Leu Trp Ile Met Tyr Ser Asn Glu 
865                 870                 875                 880 


Glu Ala Gly Ser Gly Gly Ser Val Gly Ile Ile Phe Lys Asn Gly Asp 
                885                 890                 895     


Asp Leu Arg Gln Asp Met Leu Thr Leu Gln Met Ile Gln Leu Met Asp 
            900                 905                 910         


Val Leu Trp Lys Gln Glu Gly Leu Asp Leu Arg Met Thr Pro Tyr Gly 
        915                 920                 925             


Cys Leu Pro Thr Gly Asp Arg Thr Gly Leu Ile Glu Val Val Leu Arg 
    930                 935                 940                 


Ser Asp Thr Ile Ala Asn Ile Gln Leu Asn Lys Ser Asn Met Ala Ala 
945                 950                 955                 960 


Thr Ala Ala Phe Asn Lys Asp Ala Leu Leu Asn Trp Leu Lys Ser Lys 
                965                 970                 975     


Asn Pro Gly Glu Ala Leu Asp Arg Ala Ile Glu Glu Phe Thr Leu Ser 
            980                 985                 990         


Cys Ala Gly Tyr Cys Val Ala Thr  Tyr Val Leu Gly Ile  Gly Asp Arg 
        995                 1000                 1005             


His Ser  Asp Asn Ile Met Ile  Arg Glu Ser Gly Gln  Leu Phe His 
    1010                 1015                 1020             


Ile Asp  Phe Gly His Phe Leu  Gly Asn Phe Lys Thr  Lys Phe Gly 
    1025                 1030                 1035             


Ile Asn  Arg Glu Arg Val Pro  Phe Ile Leu Thr Tyr  Asp Phe Val 
    1040                 1045                 1050             


His Val  Ile Gln Gln Gly Lys  Thr Asn Asn Ser Glu  Lys Phe Glu 
    1055                 1060                 1065             


Arg Phe  Arg Gly Tyr Cys Glu  Arg Ala Tyr Thr Ile  Leu Arg Arg 
    1070                 1075                 1080             


His Gly  Leu Leu Phe Leu His  Leu Phe Ala Leu Met  Arg Ala Ala 
    1085                 1090                 1095             


Gly Leu  Pro Glu Leu Ser Cys  Ser Lys Asp Ile Gln  Tyr Leu Lys 
    1100                 1105                 1110             


Asp Ser  Leu Ala Leu Gly Lys  Thr Glu Glu Glu Ala  Leu Lys His 
    1115                 1120                 1125             


Phe Arg  Val Lys Phe Asn Glu  Ala Leu Arg Glu Ser  Trp Lys Thr 
    1130                 1135                 1140             


Lys Val  Asn Trp Leu Ala His  Asn Val Ser Lys Asp  Asn Arg Gln 
    1145                 1150                 1155             


Glu Leu  Gly Gly Ala His His  His His His His 
    1160                 1165                 


<210>  21
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  21
ctctctgaaa tcactaagca ggagaaagat ttt                                  33


<210>  22
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  22
aaaatctttc tcctgcttag tgatttcaga gag                                  33


<210>  23
<211>  1180
<212>  PRT
<213>  Artificial

<220>
<223>  PI3K kinase construct

<400>  23

Met Arg Glu Tyr Asp Arg Leu Tyr Glu Glu Tyr Thr Arg Thr Ser Gln 
1               5                   10                  15      


Glu Ile Gln Met Lys Arg Thr Ala Ile Glu Ala Phe Asn Glu Thr Ile 
            20                  25                  30          


Lys Ile Phe Glu Glu Gln Cys Gln Thr Gln Glu Arg Tyr Ser Lys Glu 
        35                  40                  45              


Tyr Ile Glu Lys Phe Lys Arg Glu Gly Asn Glu Lys Glu Ile Gln Arg 
    50                  55                  60                  


Ile Met His Asn Tyr Asp Lys Leu Lys Ser Arg Ile Ser Glu Ile Ile 
65                  70                  75                  80  


Asp Ser Arg Arg Arg Leu Glu Glu Asp Leu Lys Lys Gln Ala Ala Glu 
                85                  90                  95      


Tyr Arg Glu Ile Asp Lys Arg Met Asn Ser Ile Lys Pro Gly Gly Ile 
            100                 105                 110         


Ser Gly Gly Gly Gly Gly Ile Met Val Leu Val Glu Cys Leu Leu Pro 
        115                 120                 125             


Asn Gly Met Ile Val Thr Leu Glu Cys Leu Arg Glu Ala Thr Leu Ile 
    130                 135                 140                 


Thr Ile Lys His Glu Leu Phe Lys Glu Ala Arg Lys Tyr Pro Leu His 
145                 150                 155                 160 


Gln Leu Leu Gln Asp Glu Ser Ser Tyr Ile Phe Val Ser Val Thr Gln 
                165                 170                 175     


Glu Ala Glu Arg Glu Glu Phe Phe Asp Glu Thr Arg Arg Leu Cys Asp 
            180                 185                 190         


Leu Arg Leu Phe Gln Pro Phe Leu Lys Val Ile Glu Pro Val Gly Asn 
        195                 200                 205             


Arg Glu Glu Lys Ile Leu Asn Arg Glu Ile Gly Phe Ala Ile Gly Met 
    210                 215                 220                 


Pro Val Cys Glu Phe Asp Met Val Lys Asp Pro Glu Val Gln Asp Phe 
225                 230                 235                 240 


Arg Arg Asn Ile Leu Asn Val Cys Lys Glu Ala Val Asp Leu Arg Asp 
                245                 250                 255     


Leu Asn Ser Pro His Ser Arg Ala Met Tyr Val Tyr Pro Pro Asn Val 
            260                 265                 270         


Glu Ser Ser Pro Glu Leu Pro Lys His Ile Tyr Asn Lys Leu Asp Lys 
        275                 280                 285             


Gly Gln Ile Ile Val Val Ile Trp Val Ile Val Ser Pro Asn Asn Asp 
    290                 295                 300                 


Lys Gln Lys Tyr Thr Leu Lys Ile Asn His Asp Cys Val Pro Glu Gln 
305                 310                 315                 320 


Val Ile Ala Glu Ala Ile Arg Lys Lys Thr Arg Ser Met Leu Leu Ser 
                325                 330                 335     


Ser Glu Gln Leu Lys Leu Cys Val Leu Glu Tyr Gln Gly Lys Tyr Ile 
            340                 345                 350         


Leu Lys Val Cys Gly Cys Asp Glu Tyr Phe Leu Glu Lys Tyr Pro Leu 
        355                 360                 365             


Ser Gln Tyr Lys Tyr Ile Arg Ser Cys Ile Met Leu Gly Arg Met Pro 
    370                 375                 380                 


Asn Leu Met Leu Met Ala Lys Glu Ser Leu Tyr Ser Gln Leu Pro Met 
385                 390                 395                 400 


Asp Cys Phe Thr Met Pro Ser Tyr Ser Arg Arg Ile Ser Thr Ala Thr 
                405                 410                 415     


Pro Tyr Met Asn Gly Glu Thr Ser Thr Lys Ser Leu Trp Val Ile Asn 
            420                 425                 430         


Ser Ala Leu Arg Ile Lys Ile Leu Cys Ala Thr Tyr Val Asn Val Asn 
        435                 440                 445             


Ile Arg Asp Ile Asp Lys Ile Tyr Val Arg Thr Gly Ile Tyr His Gly 
    450                 455                 460                 


Gly Glu Pro Leu Cys Asp Asn Val Asn Thr Gln Arg Val Pro Cys Ser 
465                 470                 475                 480 


Asn Pro Arg Trp Asn Glu Trp Leu Asn Tyr Asp Ile Tyr Ile Pro Asp 
                485                 490                 495     


Leu Pro Arg Ala Ala Arg Leu Cys Leu Ser Ile Cys Ser Val Lys Gly 
            500                 505                 510         


Arg Lys Gly Ala Lys Glu Glu His Cys Pro Leu Ala Trp Gly Asn Ile 
        515                 520                 525             


Asn Leu Phe Asp Tyr Thr Asp Thr Leu Val Ser Gly Lys Met Ala Leu 
    530                 535                 540                 


Asn Leu Trp Pro Val Pro His Gly Leu Glu Asp Leu Leu Asn Pro Ile 
545                 550                 555                 560 


Gly Val Thr Gly Ser Asn Pro Asn Lys Glu Thr Pro Cys Leu Glu Leu 
                565                 570                 575     


Glu Phe Asp Trp Phe Ser Ser Val Val Lys Phe Pro Asp Met Ser Val 
            580                 585                 590         


Ile Glu Glu His Ala Asn Trp Ser Val Ser Arg Glu Ala Gly Phe Ser 
        595                 600                 605             


Tyr Ser His Ala Gly Leu Ser Asn Arg Leu Ala Arg Asp Asn Glu Leu 
    610                 615                 620                 


Arg Glu Asn Asp Lys Glu Gln Leu Lys Ala Ile Ser Thr Arg Asp Pro 
625                 630                 635                 640 


Leu Ser Glu Ile Thr Lys Gln Glu Lys Asp Phe Leu Trp Ser His Arg 
                645                 650                 655     


His Tyr Cys Val Thr Ile Pro Glu Ile Leu Pro Lys Leu Leu Leu Ser 
            660                 665                 670         


Val Lys Trp Asn Ser Arg Asp Glu Val Ala Gln Met Tyr Cys Leu Val 
        675                 680                 685             


Lys Asp Trp Pro Pro Ile Lys Pro Glu Gln Ala Met Glu Leu Leu Asp 
    690                 695                 700                 


Cys Asn Tyr Pro Asp Pro Met Val Arg Gly Phe Ala Val Arg Cys Leu 
705                 710                 715                 720 


Glu Lys Tyr Leu Thr Asp Asp Lys Leu Ser Gln Tyr Leu Ile Gln Leu 
                725                 730                 735     


Val Gln Val Leu Lys Tyr Glu Gln Tyr Leu Asp Asn Leu Leu Val Arg 
            740                 745                 750         


Phe Leu Leu Lys Lys Ala Leu Thr Asn Gln Arg Ile Gly His Phe Phe 
        755                 760                 765             


Phe Trp His Leu Lys Ser Glu Met His Asn Lys Thr Val Ser Gln Arg 
    770                 775                 780                 


Phe Gly Leu Leu Leu Glu Ser Tyr Cys Arg Ala Cys Gly Met Tyr Leu 
785                 790                 795                 800 


Lys His Leu Asn Arg Gln Val Glu Ala Met Glu Lys Leu Ile Asn Leu 
                805                 810                 815     


Thr Asp Ile Leu Lys Gln Glu Lys Lys Asp Glu Thr Gln Lys Val Gln 
            820                 825                 830         


Met Lys Phe Leu Val Glu Gln Met Arg Arg Pro Asp Phe Met Asp Ala 
        835                 840                 845             


Leu Gln Gly Phe Leu Ser Pro Leu Asn Pro Ala His Gln Leu Gly Asn 
    850                 855                 860                 


Leu Arg Leu Glu Glu Cys Arg Ile Met Ser Ser Ala Lys Arg Pro Leu 
865                 870                 875                 880 


Trp Leu Asn Trp Glu Asn Pro Asp Ile Met Ser Glu Leu Leu Phe Gln 
                885                 890                 895     


Asn Asn Glu Ile Ile Phe Lys Asn Gly Asp Asp Leu Arg Gln Asp Met 
            900                 905                 910         


Leu Thr Leu Gln Ile Ile Arg Ile Met Glu Asn Ile Trp Gln Asn Gln 
        915                 920                 925             


Gly Leu Asp Leu Arg Met Leu Pro Tyr Gly Cys Leu Ser Ile Gly Asp 
    930                 935                 940                 


Cys Val Gly Leu Ile Glu Val Val Arg Asn Ser His Thr Ile Met Gln 
945                 950                 955                 960 


Ile Gln Cys Lys Gly Gly Leu Lys Gly Ala Leu Gln Phe Asn Ser His 
                965                 970                 975     


Thr Leu His Gln Trp Leu Lys Asp Lys Asn Lys Gly Glu Ile Tyr Asp 
            980                 985                 990         


Ala Ala Ile Asp Leu Phe Thr Arg  Ser Cys Ala Gly Tyr  Cys Val Ala 
        995                 1000                 1005             


Thr Phe  Ile Leu Gly Ile Gly  Asp Arg His Asn Ser  Asn Ile Met 
    1010                 1015                 1020             


Val Lys  Asp Asp Gly Gln Leu  Phe His Ile Asp Phe  Gly His Phe 
    1025                 1030                 1035             


Leu Asp  His Lys Lys Lys Lys  Phe Gly Tyr Lys Arg  Glu Arg Val 
    1040                 1045                 1050             


Pro Phe  Val Leu Thr Gln Asp  Phe Leu Ile Val Ile  Ser Lys Gly 
    1055                 1060                 1065             


Ala Gln  Glu Cys Thr Lys Thr  Arg Glu Phe Glu Arg  Phe Gln Glu 
    1070                 1075                 1080             


Met Cys  Tyr Lys Ala Tyr Leu  Ala Ile Arg Gln His  Ala Asn Leu 
    1085                 1090                 1095             


Phe Ile  Asn Leu Phe Ser Met  Met Leu Gly Ser Gly  Met Pro Glu 
    1100                 1105                 1110             


Leu Gln  Ser Phe Asp Asp Ile  Ala Tyr Ile Arg Lys  Thr Leu Ala 
    1115                 1120                 1125             


Leu Asp  Lys Thr Glu Gln Glu  Ala Leu Glu Tyr Phe  Met Lys Gln 
    1130                 1135                 1140             


Met Asn  Asp Ala His His Gly  Gly Trp Thr Thr Lys  Met Asp Trp 
    1145                 1150                 1155             


Ile Phe  His Thr Ile Lys Gln  His Ala Leu Asn Glu  Leu Gly Gly 
    1160                 1165                 1170             


Ala His  His His His His His  
    1175                 1180 


<210>  24
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  24
caaatgaatg atgcacgtca tggtggctgg aca                                  33


<210>  25
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  25
tgtccagcca ccatgacgtg catcattcat ttg                                  33


<210>  26
<211>  1180
<212>  PRT
<213>  Artificial

<220>
<223>  PI3K kinase construct

<400>  26

Met Arg Glu Tyr Asp Arg Leu Tyr Glu Glu Tyr Thr Arg Thr Ser Gln 
1               5                   10                  15      


Glu Ile Gln Met Lys Arg Thr Ala Ile Glu Ala Phe Asn Glu Thr Ile 
            20                  25                  30          


Lys Ile Phe Glu Glu Gln Cys Gln Thr Gln Glu Arg Tyr Ser Lys Glu 
        35                  40                  45              


Tyr Ile Glu Lys Phe Lys Arg Glu Gly Asn Glu Lys Glu Ile Gln Arg 
    50                  55                  60                  


Ile Met His Asn Tyr Asp Lys Leu Lys Ser Arg Ile Ser Glu Ile Ile 
65                  70                  75                  80  


Asp Ser Arg Arg Arg Leu Glu Glu Asp Leu Lys Lys Gln Ala Ala Glu 
                85                  90                  95      


Tyr Arg Glu Ile Asp Lys Arg Met Asn Ser Ile Lys Pro Gly Gly Ile 
            100                 105                 110         


Ser Gly Gly Gly Gly Gly Ile Met Val Leu Val Glu Cys Leu Leu Pro 
        115                 120                 125             


Asn Gly Met Ile Val Thr Leu Glu Cys Leu Arg Glu Ala Thr Leu Ile 
    130                 135                 140                 


Thr Ile Lys His Glu Leu Phe Lys Glu Ala Arg Lys Tyr Pro Leu His 
145                 150                 155                 160 


Gln Leu Leu Gln Asp Glu Ser Ser Tyr Ile Phe Val Ser Val Thr Gln 
                165                 170                 175     


Glu Ala Glu Arg Glu Glu Phe Phe Asp Glu Thr Arg Arg Leu Cys Asp 
            180                 185                 190         


Leu Arg Leu Phe Gln Pro Phe Leu Lys Val Ile Glu Pro Val Gly Asn 
        195                 200                 205             


Arg Glu Glu Lys Ile Leu Asn Arg Glu Ile Gly Phe Ala Ile Gly Met 
    210                 215                 220                 


Pro Val Cys Glu Phe Asp Met Val Lys Asp Pro Glu Val Gln Asp Phe 
225                 230                 235                 240 


Arg Arg Asn Ile Leu Asn Val Cys Lys Glu Ala Val Asp Leu Arg Asp 
                245                 250                 255     


Leu Asn Ser Pro His Ser Arg Ala Met Tyr Val Tyr Pro Pro Asn Val 
            260                 265                 270         


Glu Ser Ser Pro Glu Leu Pro Lys His Ile Tyr Asn Lys Leu Asp Lys 
        275                 280                 285             


Gly Gln Ile Ile Val Val Ile Trp Val Ile Val Ser Pro Asn Asn Asp 
    290                 295                 300                 


Lys Gln Lys Tyr Thr Leu Lys Ile Asn His Asp Cys Val Pro Glu Gln 
305                 310                 315                 320 


Val Ile Ala Glu Ala Ile Arg Lys Lys Thr Arg Ser Met Leu Leu Ser 
                325                 330                 335     


Ser Glu Gln Leu Lys Leu Cys Val Leu Glu Tyr Gln Gly Lys Tyr Ile 
            340                 345                 350         


Leu Lys Val Cys Gly Cys Asp Glu Tyr Phe Leu Glu Lys Tyr Pro Leu 
        355                 360                 365             


Ser Gln Tyr Lys Tyr Ile Arg Ser Cys Ile Met Leu Gly Arg Met Pro 
    370                 375                 380                 


Asn Leu Met Leu Met Ala Lys Glu Ser Leu Tyr Ser Gln Leu Pro Met 
385                 390                 395                 400 


Asp Cys Phe Thr Met Pro Ser Tyr Ser Arg Arg Ile Ser Thr Ala Thr 
                405                 410                 415     


Pro Tyr Met Asn Gly Glu Thr Ser Thr Lys Ser Leu Trp Val Ile Asn 
            420                 425                 430         


Ser Ala Leu Arg Ile Lys Ile Leu Cys Ala Thr Tyr Val Asn Val Asn 
        435                 440                 445             


Ile Arg Asp Ile Asp Lys Ile Tyr Val Arg Thr Gly Ile Tyr His Gly 
    450                 455                 460                 


Gly Glu Pro Leu Cys Asp Asn Val Asn Thr Gln Arg Val Pro Cys Ser 
465                 470                 475                 480 


Asn Pro Arg Trp Asn Glu Trp Leu Asn Tyr Asp Ile Tyr Ile Pro Asp 
                485                 490                 495     


Leu Pro Arg Ala Ala Arg Leu Cys Leu Ser Ile Cys Ser Val Lys Gly 
            500                 505                 510         


Arg Lys Gly Ala Lys Glu Glu His Cys Pro Leu Ala Trp Gly Asn Ile 
        515                 520                 525             


Asn Leu Phe Asp Tyr Thr Asp Thr Leu Val Ser Gly Lys Met Ala Leu 
    530                 535                 540                 


Asn Leu Trp Pro Val Pro His Gly Leu Glu Asp Leu Leu Asn Pro Ile 
545                 550                 555                 560 


Gly Val Thr Gly Ser Asn Pro Asn Lys Glu Thr Pro Cys Leu Glu Leu 
                565                 570                 575     


Glu Phe Asp Trp Phe Ser Ser Val Val Lys Phe Pro Asp Met Ser Val 
            580                 585                 590         


Ile Glu Glu His Ala Asn Trp Ser Val Ser Arg Glu Ala Gly Phe Ser 
        595                 600                 605             


Tyr Ser His Ala Gly Leu Ser Asn Arg Leu Ala Arg Asp Asn Glu Leu 
    610                 615                 620                 


Arg Glu Asn Asp Lys Glu Gln Leu Lys Ala Ile Ser Thr Arg Asp Pro 
625                 630                 635                 640 


Leu Ser Glu Ile Thr Glu Gln Glu Lys Asp Phe Leu Trp Ser His Arg 
                645                 650                 655     


His Tyr Cys Val Thr Ile Pro Glu Ile Leu Pro Lys Leu Leu Leu Ser 
            660                 665                 670         


Val Lys Trp Asn Ser Arg Asp Glu Val Ala Gln Met Tyr Cys Leu Val 
        675                 680                 685             


Lys Asp Trp Pro Pro Ile Lys Pro Glu Gln Ala Met Glu Leu Leu Asp 
    690                 695                 700                 


Cys Asn Tyr Pro Asp Pro Met Val Arg Gly Phe Ala Val Arg Cys Leu 
705                 710                 715                 720 


Glu Lys Tyr Leu Thr Asp Asp Lys Leu Ser Gln Tyr Leu Ile Gln Leu 
                725                 730                 735     


Val Gln Val Leu Lys Tyr Glu Gln Tyr Leu Asp Asn Leu Leu Val Arg 
            740                 745                 750         


Phe Leu Leu Lys Lys Ala Leu Thr Asn Gln Arg Ile Gly His Phe Phe 
        755                 760                 765             


Phe Trp His Leu Lys Ser Glu Met His Asn Lys Thr Val Ser Gln Arg 
    770                 775                 780                 


Phe Gly Leu Leu Leu Glu Ser Tyr Cys Arg Ala Cys Gly Met Tyr Leu 
785                 790                 795                 800 


Lys His Leu Asn Arg Gln Val Glu Ala Met Glu Lys Leu Ile Asn Leu 
                805                 810                 815     


Thr Asp Ile Leu Lys Gln Glu Lys Lys Asp Glu Thr Gln Lys Val Gln 
            820                 825                 830         


Met Lys Phe Leu Val Glu Gln Met Arg Arg Pro Asp Phe Met Asp Ala 
        835                 840                 845             


Leu Gln Gly Phe Leu Ser Pro Leu Asn Pro Ala His Gln Leu Gly Asn 
    850                 855                 860                 


Leu Arg Leu Glu Glu Cys Arg Ile Met Ser Ser Ala Lys Arg Pro Leu 
865                 870                 875                 880 


Trp Leu Asn Trp Glu Asn Pro Asp Ile Met Ser Glu Leu Leu Phe Gln 
                885                 890                 895     


Asn Asn Glu Ile Ile Phe Lys Asn Gly Asp Asp Leu Arg Gln Asp Met 
            900                 905                 910         


Leu Thr Leu Gln Ile Ile Arg Ile Met Glu Asn Ile Trp Gln Asn Gln 
        915                 920                 925             


Gly Leu Asp Leu Arg Met Leu Pro Tyr Gly Cys Leu Ser Ile Gly Asp 
    930                 935                 940                 


Cys Val Gly Leu Ile Glu Val Val Arg Asn Ser His Thr Ile Met Gln 
945                 950                 955                 960 


Ile Gln Cys Lys Gly Gly Leu Lys Gly Ala Leu Gln Phe Asn Ser His 
                965                 970                 975     


Thr Leu His Gln Trp Leu Lys Asp Lys Asn Lys Gly Glu Ile Tyr Asp 
            980                 985                 990         


Ala Ala Ile Asp Leu Phe Thr Arg  Ser Cys Ala Gly Tyr  Cys Val Ala 
        995                 1000                 1005             


Thr Phe  Ile Leu Gly Ile Gly  Asp Arg His Asn Ser  Asn Ile Met 
    1010                 1015                 1020             


Val Lys  Asp Asp Gly Gln Leu  Phe His Ile Asp Phe  Gly His Phe 
    1025                 1030                 1035             


Leu Asp  His Lys Lys Lys Lys  Phe Gly Tyr Lys Arg  Glu Arg Val 
    1040                 1045                 1050             


Pro Phe  Val Leu Thr Gln Asp  Phe Leu Ile Val Ile  Ser Lys Gly 
    1055                 1060                 1065             


Ala Gln  Glu Cys Thr Lys Thr  Arg Glu Phe Glu Arg  Phe Gln Glu 
    1070                 1075                 1080             


Met Cys  Tyr Lys Ala Tyr Leu  Ala Ile Arg Gln His  Ala Asn Leu 
    1085                 1090                 1095             


Phe Ile  Asn Leu Phe Ser Met  Met Leu Gly Ser Gly  Met Pro Glu 
    1100                 1105                 1110             


Leu Gln  Ser Phe Asp Asp Ile  Ala Tyr Ile Arg Lys  Thr Leu Ala 
    1115                 1120                 1125             


Leu Asp  Lys Thr Glu Gln Glu  Ala Leu Glu Tyr Phe  Met Lys Gln 
    1130                 1135                 1140             


Met Asn  Asp Ala Arg His Gly  Gly Trp Thr Thr Lys  Met Asp Trp 
    1145                 1150                 1155             


Ile Phe  His Thr Ile Lys Gln  His Ala Leu Asn Glu  Leu Gly Gly 
    1160                 1165                 1170             


Ala His  His His His His His  
    1175                 1180 


