                               SEQUENCE LISTING

<110> UNIVERSITY OF MIAMI
 
<120> OPEN-READING-FRAME (ORF) PHAGE DISPLAY

<130> 21331/2209586-WO0

<140> PCT/US09/46696
<141> 2009-06-08

<150> 61/096,015
<151> 2008-09-11

<160> 51    

<170> PatentIn version 3.5

<210> 1
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 6xHis tag

<400> 1
His His His His His His 
1               5       


<210> 2
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 2
Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210> 3
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 3
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210> 4
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 4
Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
1               5                   10      


<210> 5
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 5
Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp 
1               5                   10      


<210> 6
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 6
Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210> 7
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 7
Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210> 8
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 8
tagcggccgc taatgacttc caagccgcat tc                                     32


<210> 9
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 9
agctcgagct cgcaggccag cttgctgtc                                         29


<210> 10
<211> 104
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide

<400> 10
gcggatccgc ggccgctata actcgagggt agtgggagcg gattaaatga tatatttgag       60

gcacaaaaaa ttgaatggca ttaagaattc aagcttgtcg acgc                       104


<210> 11
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 11
cgggatccgc ggccgctata actcgagggt agtgggagcg gattaaatg                   49


<210> 12
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 12
agtgggagcg gattaaatga tatatttgag gcacaaaaaa ttgaatg                     47


<210> 13
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 13
acgtcgacaa gcttgaattc ttaatgccat tcaatttttt gtgcctc                     47


<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 14
ccaaagaatc ggttgttgaa                                                   20


<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 15
agcaaactac gctgctctga                                                   20


<210> 16
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 16
actgtaagga atccagtcgg a                                                 21


<210> 17
<211> 152
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide

<400> 17
cgggatccgc tggaagtgct gtttcagggc ccgggcagcg gttcaggctc gcggccgcta       60

gatatctaac tcgagggtag tgggagcgga ttaaatgata tatttgaggc acaaaaaatt      120

gaatggcatt aagaattcaa gcttgtcgac gt                                    152


<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer


<220>
<221> modified_base
<222> (12)..(20)
<223> a, c, t, g, unknown or other

<400> 18
gcgccgcgac cnnnnnnnnn                                                   20


<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer


<220>
<221> modified_base
<222> (12)..(20)
<223> a, c, t, g, unknown or other 

<400> 19
ggccggcctc cnnnnnnnnn                                                   20


<210> 20
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 20
aactagataa gaatagcggc cgcgcgccgc gacc                                   34


<210> 21
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 21
aactagataa gaatagcggc cgcagcgccg cgacc                                  35


<210> 22
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 22
aactagataa gaatagcggc cgcaagcgcc gcgacc                                 36


<210> 23
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 23
aatgatctcg agggccggcc tcc                                               23


<210> 24
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 24
aatgatctcg agtggccggc ctcc                                              24


<210> 25
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 25
aatgatctcg agtaggccgg cctcc                                             25


<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 26
ccaagcggac cagattatcg                                                   20


<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 27
gagcgcatat agttcctcct                                                   20


<210> 28
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 28
tgcggatatc tcggtagtg                                                    19


<210> 29
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 29
atgaattctc tagagtcgag gaatactgtt tcctgtgtg                              39


<210> 30
<211> 104
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide

<400> 30
gcggatccgc ggccgctata actcgagggt agtgggagcg gattaaatga tatatttgag       60

gcacaaaaaa ttgaatggca ttaagaattc aagcttgtcg acaa                       104


<210> 31
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 31
cagcggccgc tggaccagtg caccccaaac cct                                    33


<210> 32
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 32
acctcgaggg gggtggcatg ctccacag                                          28


<210> 33
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 33
cagcggccgc tgatgcaggt ctcccgtgtg ctg                                    33


<210> 34
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 34
gtctcgagac agcccagcag ctccaggcg                                         29


<210> 35
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 35
tcccactcct gcccgcctgt                                                   20


<210> 36
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 36
gggtggggac ggcagtattg                                                   20


<210> 37
<211> 162
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide


<220>
<221> CDS
<222> (1)..(75)

<220>
<221> CDS
<222> (79)..(138)

<400> 37
gtg atg ctc ggg gat ccg ctg gaa gtg ctg ttt cag ggc ccg ggc agc         48
Val Met Leu Gly Asp Pro Leu Glu Val Leu Phe Gln Gly Pro Gly Ser           
1               5                   10                  15                

ggt tca ggc tcg cgg ccg cta gat atc taa ctc gag ggt agt ggg agc         96
Gly Ser Gly Ser Arg Pro Leu Asp Ile     Leu Glu Gly Ser Gly Ser           
            20                  25                      30                

gga tta aat gat ata ttt gag gca caa aaa att gaa tgg cat                138
Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His                   
            35                  40                  45                    

taagaattca agcttgtcga gtaa                                             162


<210> 38
<211> 25
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 38
Val Met Leu Gly Asp Pro Leu Glu Val Leu Phe Gln Gly Pro Gly Ser 
1               5                   10                  15      


Gly Ser Gly Ser Arg Pro Leu Asp Ile 
            20                  25  


<210> 39
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 39
Leu Glu Gly Ser Gly Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
1               5                   10                  15      


Ile Glu Trp His 
            20  


<210> 40
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 40
Lys Gly Val Lys Lys Lys 
1               5       


<210> 41
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 41
Lys Gly Val Lys Ala Ala 
1               5       


<210> 42
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 42
Lys Gly Lys Gly Lys Lys Lys 
1               5           


<210> 43
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 43
Lys Gly Lys Gly Lys Ala Ala 
1               5           


<210> 44
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 44
Ala Gly Ala Gly Lys Lys Lys 
1               5           


<210> 45
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 45
Ala Gly Ala Gly Ala Lys Lys 
1               5           


<210> 46
<211> 111
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide


<220>
<221> CDS
<222> (1)..(27)

<220>
<221> CDS
<222> (31)..(90)

<400> 46
gtg atg ctc ggg gat ccg cgg ccg cta taa ctc gag ggt agt ggg agc         48
Val Met Leu Gly Asp Pro Arg Pro Leu     Leu Glu Gly Ser Gly Ser           
1               5                       10                  15            

gga tta aat gat ata ttt gag gca caa aaa att gaa tgg cat                 90
Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His                   
                20                  25                                    

taagaattca agcttgtcga g                                                111


<210> 47
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 47
Val Met Leu Gly Asp Pro Arg Pro Leu 
1               5                   


<210> 48
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 48
Leu Glu Gly Ser Gly Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
1               5                   10                  15      


Ile Glu Trp His 
            20  


<210> 49
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 49
ggagctgtcg tattccagtc                                                   20


<210> 50
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 50
aacccctcaa gacccgttta                                                   20


<210> 51
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 8xHis tag

<400> 51
His His His His His His His His 
1               5               


