                         SEQUENCE LISTING

<110>  Mucosis B.V.
 
<120>  Multifunctional linker protein

<130>  P85439EP00

<140>  EP08164785.1
<141>  2008-09-22

<160>  10    

<170>  PatentIn version 3.3

<210>  1
<211>  252
<212>  PRT
<213>  Artificial

<220>
<223>  scHC45


<220>
<221>  DOMAIN
<222>  (1)..(112)
<223>  HC45 VL

<220>
<221>  DOMAIN
<222>  (113)..(130)
<223>  "218" linker

<220>
<221>  DOMAIN
<222>  (131)..(252)
<223>  HC45 VH

<400>  1

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Thr Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
        115                 120                 125             


Lys Gly Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Arg Pro 
    130                 135                 140                 


Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Leu Thr 
145                 150                 155                 160 


Thr Tyr Trp Met Asn Trp Phe Lys Gln Arg Pro Asp Gln Gly Leu Glu 
                165                 170                 175     


Trp Ile Gly Arg Ile Asp Pro Tyr Asp Ser Glu Thr His Tyr Asn Gln 
            180                 185                 190         


Lys Phe Lys Asp Lys Ala Ile Leu Thr Val Asp Arg Ser Ser Ser Thr 
        195                 200                 205             


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
    210                 215                 220                 


Tyr Cys Thr Arg Phe Leu Gln Ile Thr Thr Ile Ile Tyr Gly Met Asp 
225                 230                 235                 240 


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
                245                 250         


<210>  2
<211>  129
<212>  PRT
<213>  Artificial

<220>
<223>  camelised VH45


<220>
<221>  DOMAIN
<222>  (1)..(122)
<223>  VH domain

<220>
<221>  DOMAIN
<222>  (123)..(129)
<223>  linker

<400>  2

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Ser Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Leu Thr Thr Tyr 
            20                  25                  30          


Trp Met Asn Trp Phe Lys Gln Arg Pro Asp Gln Glu Arg Glu Gly Ile 
        35                  40                  45              


Gly Arg Ile Asp Pro Tyr Asp Ser Glu Thr His Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Ile Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Leu Gln Ile Thr Thr Ile Ile Tyr Gly Met Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro 
        115                 120                 125             


Ser 
    


<210>  3
<211>  18
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  3

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  4
<211>  43
<212>  PRT
<213>  Lactococcus lactis

<400>  4

Tyr Thr Val Lys Ser Gly Asp Thr Leu Trp Gly Ile Ser Gln Arg Tyr 
1               5                   10                  15      


Gly Ile Ser Val Ala Gln Ile Gln Ser Ala Asn Asn Leu Lys Ser Thr 
            20                  25                  30          


Ile Ile Tyr Ile Gly Gln Lys Leu Val Leu Thr 
        35                  40              


<210>  5
<211>  43
<212>  PRT
<213>  Lactococcus lactis

<400>  5

Val Lys Val Lys Ser Gly Asp Thr Leu Trp Ala Leu Ser Val Lys Tyr 
1               5                   10                  15      


Lys Thr Ser Ile Ala Gln Leu Lys Ser Trp Asn His Leu Ser Ser Asp 
            20                  25                  30          


Thr Ile Tyr Ile Gly Gln Asn Leu Ile Val Ser 
        35                  40              


<210>  6
<211>  43
<212>  PRT
<213>  Lactococcus lactis

<400>  6

His Lys Val Val Lys Gly Asp Thr Leu Trp Gly Leu Ser Gln Lys Ser 
1               5                   10                  15      


Gly Ser Pro Ile Ala Ser Ile Lys Ala Trp Asn His Leu Ser Ser Asp 
            20                  25                  30          


Thr Ile Leu Ile Gly Gln Tyr Leu Arg Ile Lys 
        35                  40              


<210>  7
<211>  43
<212>  PRT
<213>  Artificial

<220>
<223>  consensus AcmA 1/4


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  may be H

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  may be L or I

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  may be R

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  may be E

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  may be S

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  may be V or I

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  may be A

<220>
<221>  misc_feature
<222>  (14)..(22)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  may be L

<220>
<221>  misc_feature
<222>  (24)..(26)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (29)..(29)
<223>  may be I

<220>
<221>  misc_feature
<222>  (30)..(33)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (34)..(34)
<223>  may be L or V

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (39)..(39)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (40)..(40)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (42)..(42)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Xaa can be any naturally occurring amino acid

<400>  7

Tyr Xaa Val Lys Xaa Gly Asp Thr Leu Xaa Xaa Leu Ser Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Ile Xaa Xaa Xaa Asn Xaa Leu Xaa Xaa Xaa 
            20                  25                  30          


Xaa Ile Xaa Xaa Gly Gln Xaa Leu Xaa Leu Xaa 
        35                  40              


<210>  8
<211>  44
<212>  PRT
<213>  Artificial

<220>
<223>  consensus 2/4


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  may be H

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  may be L or I

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  may be R

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  may be E

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  may be S

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  may be V or I

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  may be A

<220>
<221>  misc_feature
<222>  (14)..(22)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  may be L

<220>
<221>  misc_feature
<222>  (24)..(26)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (30)..(30)
<223>  may be I

<220>
<221>  misc_feature
<222>  (31)..(34)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (35)..(35)
<223>  may be L or V

<220>
<221>  misc_feature
<222>  (36)..(37)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (41)..(41)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa can be any naturally occurring amino acid

<400>  8

Tyr Xaa Val Lys Xaa Gly Asp Thr Leu Xaa Xaa Leu Ser Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Ile Xaa Xaa Xaa Asn Xaa Xaa Leu Xaa Xaa 
            20                  25                  30          


Xaa Xaa Ile Xaa Xaa Gly Gln Xaa Leu Xaa Leu Xaa 
        35                  40                  


<210>  9
<211>  44
<212>  PRT
<213>  Artificial

<220>
<223>  consensus 3/4


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  may be H

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  may be L or I

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  may be R

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  may be E

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  may be S

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  may be V or I

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  may be A

<220>
<221>  misc_feature
<222>  (14)..(22)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  may be L

<220>
<221>  misc_feature
<222>  (24)..(26)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (29)..(29)
<223>  may be I

<220>
<221>  misc_feature
<222>  (30)..(34)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (35)..(35)
<223>  may be L or V

<220>
<221>  misc_feature
<222>  (36)..(37)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (41)..(41)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa can be any naturally occurring amino acid

<400>  9

Tyr Xaa Val Lys Xaa Gly Asp Thr Leu Xaa Xaa Leu Ser Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Ile Xaa Xaa Xaa Asn Xaa Leu Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Ile Xaa Xaa Gly Gln Xaa Leu Xaa Leu Xaa 
        35                  40                  


<210>  10
<211>  45
<212>  PRT
<213>  Artificial

<220>
<223>  consensus 4/4


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  may be H

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  may be L or I

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  may be R

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  may be E

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  may be S

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  may be V or I

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  may be A

<220>
<221>  misc_feature
<222>  (14)..(22)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  may be L

<220>
<221>  misc_feature
<222>  (24)..(26)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (30)..(30)
<223>  may be I

<220>
<221>  misc_feature
<222>  (31)..(35)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (36)..(36)
<223>  may be L or V

<220>
<221>  misc_feature
<222>  (37)..(38)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (42)..(42)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (44)..(44)
<223>  may be V or I

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  Xaa can be any naturally occurring amino acid

<400>  10

Tyr Xaa Val Lys Xaa Gly Asp Thr Leu Xaa Xaa Leu Ser Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Ile Xaa Xaa Xaa Asn Xaa Xaa Leu Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Ile Xaa Xaa Gly Gln Xaa Leu Xaa Leu Xaa 
        35                  40                  45  


