                         SEQUENCE LISTING

<110>  Shirtliff, Mark E.
       O'May, Graeme
       Brady, Rebecca
       Leid, Jeff
 
<120>  PROTECTIVE VACCINE AGAINST STAPHYLOCOCCUS AUREUS BIOFILMS 
       COMPRISING CELL WALL-ASSOCIATED IMMUNOGENS

<130>  70089.0031WOU1

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  1257
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Met Ala Lys Lys Phe Asn Tyr Lys Leu Pro Ser Met Val Ala Leu Thr 
1               5                   10                  15      


Leu Val Gly Ser Ala Val Thr Ala His Gln Val Gln Ala Ala Glu Thr 
            20                  25                  30          


Thr Gln Asp Gln Thr Thr Asn Lys Asn Val Leu Asp Ser Asn Lys Val 
        35                  40                  45              


Lys Ala Thr Thr Glu Gln Ala Lys Ala Glu Val Lys Asn Pro Thr Gln 
    50                  55                  60                  


Asn Ile Ser Gly Thr Gln Val Tyr Gln Asp Pro Ala Ile Val Gln Pro 
65                  70                  75                  80  


Lys Ala Ala Asn Lys Thr Gly Asn Ala Gln Val Asn Gln Lys Val Asp 
                85                  90                  95      


Thr Thr Gln Val Asn Gly Asp Thr Arg Ala Thr Gln Ser Thr Thr Ser 
            100                 105                 110         


Asn Asn Ala Lys Pro Val Thr Lys Ser Thr Asn Thr Thr Ala Pro Lys 
        115                 120                 125             


Thr Asn Asn Asn Val Thr Ser Ala Gly Tyr Ser Leu Val Asp Asp Glu 
    130                 135                 140                 


Asp Asp Asn Ser Glu Asn Gln Ile Asn Pro Glu Leu Ile Lys Ser Ala 
145                 150                 155                 160 


Ala Lys Pro Ala Ala Leu Glu Thr Gln Tyr Lys Ala Ala Ala Pro Lys 
                165                 170                 175     


Ala Thr Pro Val Ala Pro Lys Ala Lys Thr Glu Ala Thr Pro Lys Val 
            180                 185                 190         


Thr Thr Phe Ser Ala Ser Ala Gln Pro Arg Ser Ala Ala Ala Ala Pro 
        195                 200                 205             


Lys Thr Ser Leu Pro Lys Tyr Lys Pro Gln Val Asn Ser Ser Ile Asn 
    210                 215                 220                 


Asp Tyr Ile Arg Lys Asn Asn Leu Lys Ala Pro Lys Ile Glu Glu Asp 
225                 230                 235                 240 


Tyr Thr Ser Tyr Phe Pro Lys Tyr Ala Tyr Arg Asn Gly Val Gly Arg 
                245                 250                 255     


Pro Glu Gly Ile Val Val His Asp Thr Ala Asn Asp Arg Ser Thr Ile 
            260                 265                 270         


Asn Gly Glu Ile Ser Tyr Met Lys Asn Asn Tyr Gln Asn Ala Phe Val 
        275                 280                 285             


His Ala Phe Val Asp Gly Asp Arg Ile Ile Glu Thr Ala Pro Thr Asp 
    290                 295                 300                 


Tyr Leu Ser Trp Gly Val Gly Ala Val Gly Asn Pro Arg Phe Ile Asn 
305                 310                 315                 320 


Val Glu Ile Val His Thr His Asp Tyr Ala Ser Phe Ala Arg Ser Met 
                325                 330                 335     


Asn Asn Tyr Ala Asp Tyr Ala Ala Thr Gln Leu Gln Tyr Tyr Gly Leu 
            340                 345                 350         


Lys Pro Asp Ser Ala Glu Tyr Asp Gly Asn Gly Thr Val Trp Thr His 
        355                 360                 365             


Tyr Ala Val Ser Lys Tyr Leu Gly Gly Thr Asp His Ala Asp Pro His 
    370                 375                 380                 


Gly Tyr Leu Arg Ser His Asn Tyr Ser Tyr Asp Gln Leu Tyr Asp Leu 
385                 390                 395                 400 


Ile Asn Glu Lys Tyr Leu Ile Lys Met Gly Lys Val Ala Pro Trp Gly 
                405                 410                 415     


Thr Gln Ser Thr Thr Thr Pro Thr Thr Pro Ser Lys Pro Ser Thr Pro 
            420                 425                 430         


Ser Lys Pro Ser Thr Pro Ser Thr Gly Lys Leu Thr Val Ala Ala Asn 
        435                 440                 445             


Asn Gly Val Ala Gln Ile Lys Pro Thr Asn Ser Gly Leu Tyr Thr Thr 
    450                 455                 460                 


Val Tyr Asp Lys Thr Gly Lys Ala Thr Asn Glu Val Gln Lys Thr Phe 
465                 470                 475                 480 


Ala Val Ser Lys Thr Ala Thr Leu Gly Asn Gln Lys Phe Tyr Leu Val 
                485                 490                 495     


Gln Asp Tyr Asn Ser Gly Asn Lys Phe Gly Trp Val Lys Glu Gly Asp 
            500                 505                 510         


Val Val Tyr Asn Thr Ala Lys Ser Pro Val Asn Val Asn Gln Ser Tyr 
        515                 520                 525             


Ser Ile Lys Pro Gly Thr Lys Leu Tyr Thr Val Pro Trp Gly Thr Ser 
    530                 535                 540                 


Lys Gln Val Ala Gly Ser Val Ser Gly Ser Gly Asn Gln Thr Phe Lys 
545                 550                 555                 560 


Ala Ser Lys Gln Gln Gln Ile Asp Lys Ser Ile Tyr Leu Tyr Gly Ser 
                565                 570                 575     


Val Asn Gly Lys Ser Gly Trp Val Ser Lys Ala Tyr Leu Val Asp Thr 
            580                 585                 590         


Ala Lys Pro Thr Pro Thr Pro Thr Pro Lys Pro Ser Thr Pro Thr Thr 
        595                 600                 605             


Asn Asn Lys Leu Thr Val Ser Ser Leu Asn Gly Val Ala Gln Ile Asn 
    610                 615                 620                 


Ala Lys Asn Asn Gly Leu Phe Thr Thr Val Tyr Asp Lys Thr Gly Lys 
625                 630                 635                 640 


Pro Thr Lys Glu Val Gln Lys Thr Phe Ala Val Thr Lys Glu Ala Ser 
                645                 650                 655     


Leu Gly Gly Asn Lys Phe Tyr Leu Val Lys Asp Tyr Asn Ser Pro Thr 
            660                 665                 670         


Leu Ile Gly Trp Val Lys Gln Gly Asp Val Ile Tyr Asn Asn Ala Lys 
        675                 680                 685             


Ser Pro Val Asn Val Met Gln Thr Tyr Thr Val Lys Pro Gly Thr Lys 
    690                 695                 700                 


Leu Tyr Ser Val Pro Trp Gly Thr Tyr Lys Gln Glu Ala Gly Ala Val 
705                 710                 715                 720 


Ser Gly Thr Gly Asn Gln Thr Phe Lys Ala Thr Lys Gln Gln Gln Ile 
                725                 730                 735     


Asp Lys Ser Ile Tyr Leu Tyr Gly Thr Val Asn Gly Lys Ser Gly Trp 
            740                 745                 750         


Ile Ser Lys Ala Tyr Leu Ala Val Pro Ala Ala Pro Lys Lys Ala Val 
        755                 760                 765             


Ala Gln Pro Lys Thr Ala Val Lys Ala Tyr Ala Val Thr Lys Pro Gln 
    770                 775                 780                 


Thr Thr Gln Thr Val Ser Lys Ile Ala Gln Val Lys Pro Asn Asn Thr 
785                 790                 795                 800 


Gly Ile Arg Ala Ser Val Tyr Glu Lys Thr Ala Lys Asn Gly Ala Lys 
                805                 810                 815     


Tyr Ala Asp Arg Thr Phe Tyr Val Thr Lys Glu Arg Ala His Gly Asn 
            820                 825                 830         


Glu Thr Tyr Val Leu Leu Asn Asn Thr Ser His Asn Ile Pro Leu Gly 
        835                 840                 845             


Trp Phe Asn Val Lys Asp Leu Asn Val Gln Asn Leu Gly Lys Glu Val 
    850                 855                 860                 


Lys Thr Thr Gln Lys Tyr Thr Val Asn Arg Ser Asn Asn Gly Leu Ser 
865                 870                 875                 880 


Met Val Pro Trp Gly Thr Lys Asn Gln Val Ile Leu Thr Gly Asn Asn 
                885                 890                 895     


Ile Ala Gln Gly Thr Phe Asn Ala Thr Lys Gln Val Ser Val Gly Lys 
            900                 905                 910         


Asp Val Tyr Leu Tyr Gly Thr Ile Asn Asn Arg Thr Gly Trp Val Asn 
        915                 920                 925             


Ser Lys Asp Leu Thr Ala Pro Thr Ala Val Lys Pro Thr Thr Ser Ala 
    930                 935                 940                 


Ala Lys Asp Tyr Asn Tyr Thr Tyr Val Ile Lys Asn Gly Asn Gly Tyr 
945                 950                 955                 960 


Tyr Tyr Val Thr Pro Asn Ser Asp Thr Ala Lys Tyr Ser Leu Lys Ala 
                965                 970                 975     


Phe Asn Glu Gln Pro Phe Ala Val Val Lys Glu Gln Val Ile Asn Gly 
            980                 985                 990         


Gln Thr Trp Tyr Tyr Gly Lys Leu  Ser Asn Gly Lys Leu  Ala Trp Ile 
        995                 1000                 1005             


Lys Ser  Thr Asp Leu Ala Lys  Glu Leu Ile Lys Tyr  Asn Gln Ile 
    1010                 1015                 1020             


Gly Met  Thr Leu Asn Gln Val  Ala Gln Ile Gln Ala  Gly Leu Gln 
    1025                 1030                 1035             


Tyr Lys  Pro Gln Val Gln Arg  Val Pro Gly Lys Trp  Thr Asp Ala 
    1040                 1045                 1050             


Asn Phe  Asn Asp Val Lys His  Ala Met Asp Thr Lys  Arg Leu Ala 
    1055                 1060                 1065             


Gln Asp  Pro Ala Leu Lys Tyr  Gln Phe Leu Arg Leu  Asp Gln Pro 
    1070                 1075                 1080             


Gln Asn  Ile Ser Ile Asp Lys  Ile Asn Gln Phe Leu  Lys Gly Lys 
    1085                 1090                 1095             


Gly Val  Leu Glu Asn Gln Gly  Ala Ala Phe Asn Lys  Ala Ala Gln 
    1100                 1105                 1110             


Met Tyr  Gly Ile Asn Glu Val  Tyr Leu Ile Ser His  Ala Leu Leu 
    1115                 1120                 1125             


Glu Thr  Gly Asn Gly Thr Ser  Gln Leu Ala Lys Gly  Ala Asp Val 
    1130                 1135                 1140             


Val Asn  Asn Lys Val Val Thr  Asn Ser Asn Thr Lys  Tyr His Asn 
    1145                 1150                 1155             


Val Phe  Gly Ile Ala Ala Tyr  Asp Asn Asp Pro Leu  Arg Glu Gly 
    1160                 1165                 1170             


Ile Lys  Tyr Ala Lys Gln Ala  Gly Trp Asp Thr Val  Ser Lys Ala 
    1175                 1180                 1185             


Ile Val  Gly Gly Ala Lys Phe  Ile Gly Asn Ser Tyr  Val Lys Ala 
    1190                 1195                 1200             


Gly Gln  Asn Thr Leu Tyr Lys  Met Arg Trp Asn Pro  Ala His Pro 
    1205                 1210                 1215             


Gly Thr  His Gln Tyr Ala Thr  Asp Val Asp Trp Ala  Asn Ile Asn 
    1220                 1225                 1230             


Ala Lys  Ile Ile Lys Gly Tyr  Tyr Asp Lys Ile Gly  Glu Val Gly 
    1235                 1240                 1245             


Lys Tyr  Phe Asp Ile Pro Gln  Tyr Lys 
    1250                 1255         


<210>  2
<211>  309
<212>  PRT
<213>  Staphylococcus aureus

<400>  2

Met Lys Lys Leu Val Pro Leu Leu Leu Ala Leu Leu Leu Leu Val Ala 
1               5                   10                  15      


Ala Cys Gly Thr Gly Gly Lys Gln Ser Ser Asp Lys Ser Asn Gly Lys 
            20                  25                  30          


Leu Lys Val Val Thr Thr Asn Ser Ile Leu Tyr Asp Met Ala Lys Asn 
        35                  40                  45              


Val Gly Gly Asp Asn Val Asp Ile His Ser Ile Val Pro Val Gly Gln 
    50                  55                  60                  


Asp Pro His Glu Tyr Glu Val Lys Pro Lys Asp Ile Lys Lys Leu Thr 
65                  70                  75                  80  


Asp Ala Asp Val Ile Leu Tyr Asn Gly Leu Asn Leu Glu Thr Gly Asn 
                85                  90                  95      


Gly Trp Phe Glu Lys Ala Leu Glu Gln Ala Gly Lys Ser Leu Lys Asp 
            100                 105                 110         


Lys Lys Val Ile Ala Val Ser Lys Asp Val Lys Pro Ile Tyr Leu Asn 
        115                 120                 125             


Gly Glu Glu Gly Asn Lys Asp Lys Gln Asp Pro His Ala Trp Leu Ser 
    130                 135                 140                 


Leu Asp Asn Gly Ile Lys Tyr Val Lys Thr Ile Gln Gln Thr Phe Ile 
145                 150                 155                 160 


Asp Asn Asp Lys Lys His Lys Ala Asp Tyr Glu Lys Gln Gly Asn Lys 
                165                 170                 175     


Tyr Ile Ala Gln Leu Glu Lys Leu Asn Asn Asp Ser Lys Asp Lys Phe 
            180                 185                 190         


Asn Asp Ile Pro Lys Glu Gln Arg Ala Met Ile Thr Ser Glu Gly Ala 
        195                 200                 205             


Phe Lys Tyr Phe Ser Lys Gln Tyr Gly Ile Thr Pro Gly Tyr Ile Trp 
    210                 215                 220                 


Glu Ile Asn Thr Glu Lys Gln Gly Thr Pro Glu Gln Met Arg Gln Ala 
225                 230                 235                 240 


Ile Glu Phe Val Lys Lys His Lys Leu Lys His Leu Leu Val Glu Thr 
                245                 250                 255     


Ser Val Asp Lys Lys Ala Met Glu Ser Leu Ser Glu Glu Thr Lys Lys 
            260                 265                 270         


Asp Ile Phe Gly Glu Val Tyr Thr Asp Ser Ile Gly Lys Glu Gly Thr 
        275                 280                 285             


Lys Gly Asp Ser Tyr Tyr Lys Met Met Lys Ser Asn Ile Glu Thr Val 
    290                 295                 300                 


His Gly Ser Met Lys 
305                 


<210>  3
<211>  168
<212>  PRT
<213>  Staphylococcus aureus

<400>  3

Met Asn Thr Ile Lys Asn Thr Ile Tyr Thr Glu Ala Ile Phe Ser Lys 
1               5                   10                  15      


Asp Glu Lys His Arg Tyr Leu Leu Lys Lys Thr Trp Asp Glu Lys Lys 
            20                  25                  30          


Pro Ala Cys Thr Val Ile Thr Met Tyr Pro His Leu Asp Gly Val Leu 
        35                  40                  45              


Ser Leu Asp Leu Thr Thr Val Leu Ile Leu Asn Gln Leu Ala Asn Ser 
    50                  55                  60                  


Glu Arg Tyr Gly Ala Val Tyr Leu Val Asn Leu Phe Ser Asn Ile Lys 
65                  70                  75                  80  


Thr Pro Glu Asn Leu Lys His Ile Lys Glu Pro Tyr Asp Lys His Thr 
                85                  90                  95      


Asp Ile His Leu Met Lys Ala Ile Ser Glu Ser Asp Thr Val Ile Leu 
            100                 105                 110         


Ala Tyr Gly Ala Tyr Ala Lys Arg Pro Val Val Val Glu Arg Val Glu 
        115                 120                 125             


Gln Val Met Glu Met Leu Lys Pro His Lys Lys Lys Val Lys Lys Leu 
    130                 135                 140                 


Ile Asn Pro Ala Thr Asn Glu Ile Met His Pro Leu Asn Pro Lys Ala 
145                 150                 155                 160 


Arg Gln Lys Trp Thr Leu Lys Ala 
                165             


<210>  4
<211>  270
<212>  PRT
<213>  Staphylococcus aureus

<400>  4

Met Lys Tyr Lys Thr Glu Arg Arg Glu Met Met Gly Asn Ile Lys Ser 
1               5                   10                  15      


Phe Ala Leu Tyr Ile Ser Ile Leu Leu Leu Ile Val Val Val Ala Gly 
            20                  25                  30          


Cys Gly Lys Ser Asp Lys Thr Lys Glu Asp Ser Lys Glu Glu Gln Ile 
        35                  40                  45              


Lys Lys Ser Phe Ala Lys Thr Leu Asp Met Tyr Pro Ile Lys Asn Leu 
    50                  55                  60                  


Glu Asp Leu Tyr Asp Lys Glu Gly Tyr Arg Asp Gly Glu Phe Lys Lys 
65                  70                  75                  80  


Gly Asp Lys Gly Thr Trp Thr Leu Leu Thr Ser Phe Ser Lys Ser Asn 
                85                  90                  95      


Lys Pro Asp Glu Ile Asp Asp Glu Gly Met Val Leu Tyr Leu Asn Arg 
            100                 105                 110         


Asn Thr Lys Lys Ala Thr Gly Tyr Tyr Phe Val Asn Lys Ile Tyr Asp 
        115                 120                 125             


Asp Ile Ser Lys Asn Gln Asn Glu Lys Lys Tyr Arg Val Glu Leu Lys 
    130                 135                 140                 


Asn Asn Lys Ile Val Leu Leu Asp Asn Val Glu Asp Glu Lys Leu Lys 
145                 150                 155                 160 


Gln Lys Ile Glu Asn Phe Lys Phe Phe Ser Gln Tyr Ala Asp Phe Lys 
                165                 170                 175     


Asp Leu Lys Asn Tyr Gln Asp Gly Ser Ile Thr Thr Asn Glu Asn Ile 
            180                 185                 190         


Pro Ser Tyr Glu Ala Glu Tyr Lys Leu Asn Asn Ser Asp Glu Asn Val 
        195                 200                 205             


Lys Lys Leu Arg Asp Ile Tyr Pro Ile Thr Thr Lys Lys Ala Pro Ile 
    210                 215                 220                 


Leu Lys Leu His Ile Asp Gly Asp Ile Lys Gly Ser Ser Val Gly Tyr 
225                 230                 235                 240 


Lys Lys Ile Glu Tyr Lys Phe Ser Lys Val Lys Asp Gln Glu Thr Thr 
                245                 250                 255     


Leu Arg Asp Tyr Leu Asn Phe Gly Pro Ser Asp Glu Asp Ser 
            260                 265                 270 


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  5
atgaatacaa tcaaaactac gaaa                                              24


<210>  6
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  6
cttctcatcg tcatctgatt tcaaaatcca tttttga                                37


<210>  7
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  7
actctaggtc tcactcccat ctgaaacaac attatgacca aat                         43


<210>  8
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  8
atggtaggtc tcatatcata aaggatttaa cggtaattca ttact                       45


<210>  9
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  9
atggtaggtc tcactccgat aagtcaaatg gcaaactaaa agt                         43


<210>  10
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  10
atggtaggtc tcatatcatt tcatgcttcc gtgtacagtt                             40


<210>  11
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  11
atggtaggtc tcactccgct tatactgtta ctaaaccaca aac                         43


<210>  12
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  12
atggtaggtc tcatatcatt tatattgtgg gatgtcgaag tatt                        44


<210>  13
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  13
actctaggtc tcactccaaa gaagattcaa aagaagaaca aat                         43


<210>  14
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized PCR primer

<400>  14
atggtaggtc tcatatcagc tatcttcatc agacggccca                             40


