                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
       Mink, Daniel
       Guit, Rudolf Philippus Maria
       Raemaekers, Karel Gerardus Hubertus
       Mller, Monika
 
<120>  Method for preparing epsilon-caprolactam from 
       N-acetyl-6-aminocaproic acid

<130>  P85292PC00

<150>  EP 08166286.8
<151>  2008-10-09

<160>  2     

<170>  PatentIn version 3.3

<210>  1
<211>  2448
<212>  DNA
<213>  Alcaligenes faecalis


<220>
<221>  CDS
<222>  (1)..(2448)

<400>  1
atg cag aaa ggg ctt gtt cgt acc ggg ctt gtg gcc gct ggt ttg atc       48
Met Gln Lys Gly Leu Val Arg Thr Gly Leu Val Ala Ala Gly Leu Ile         
1               5                   10                  15              

ttg ggt tgg gcg ggg gca ccg acc cac gcg caa gtg cag tcg gta gag       96
Leu Gly Trp Ala Gly Ala Pro Thr His Ala Gln Val Gln Ser Val Glu         
            20                  25                  30                  

gtg atg cgg gac agt tat ggc gtg ccg cac gtc ttt gcc gac agc cac      144
Val Met Arg Asp Ser Tyr Gly Val Pro His Val Phe Ala Asp Ser His         
        35                  40                  45                      

tat ggc ttg tat tac ggc tat ggt tat gcg gtc gcc caa gac cgt ctg      192
Tyr Gly Leu Tyr Tyr Gly Tyr Gly Tyr Ala Val Ala Gln Asp Arg Leu         
    50                  55                  60                          

ttc cag atg gac atg gcg cgt cgc tcc ttt gtc ggc aca acc gcc gcc      240
Phe Gln Met Asp Met Ala Arg Arg Ser Phe Val Gly Thr Thr Ala Ala         
65                  70                  75                  80          

gtc tta ggc cct ggt gag caa gat gcc tac gtc aag tac gac atg cag      288
Val Leu Gly Pro Gly Glu Gln Asp Ala Tyr Val Lys Tyr Asp Met Gln         
                85                  90                  95              

gtg cgg cag aac ttc acc ccg gct tcc ata cag cgg cag atc gcg gcc      336
Val Arg Gln Asn Phe Thr Pro Ala Ser Ile Gln Arg Gln Ile Ala Ala         
            100                 105                 110                 

ttg tcc aag gat gag cgc gat att ttt cgt ggc tat gcc gat ggc tat      384
Leu Ser Lys Asp Glu Arg Asp Ile Phe Arg Gly Tyr Ala Asp Gly Tyr         
        115                 120                 125                     

aac gcc tat ctg gag cag gtg cgg cgt cgc cct gag ttg ctg ccc aaa      432
Asn Ala Tyr Leu Glu Gln Val Arg Arg Arg Pro Glu Leu Leu Pro Lys         
    130                 135                 140                         

gaa tat gtg gat ttt gat ttc cag ccc gag ccg ctg acc gac ttt gat      480
Glu Tyr Val Asp Phe Asp Phe Gln Pro Glu Pro Leu Thr Asp Phe Asp         
145                 150                 155                 160         

gtg gtc atg atc tgg gtg ggc tcc atg gcc aat cgc ttc tcc gac acg      528
Val Val Met Ile Trp Val Gly Ser Met Ala Asn Arg Phe Ser Asp Thr         
                165                 170                 175             

aat ctg gaa gtg acg gca ctg gcc atg cgt cag tct ctg gag aaa cag      576
Asn Leu Glu Val Thr Ala Leu Ala Met Arg Gln Ser Leu Glu Lys Gln         
            180                 185                 190                 

cac ggc ccg gaa cga ggc cgt gcc ttg ttt gat gag ctg ctg tgg atc      624
His Gly Pro Glu Arg Gly Arg Ala Leu Phe Asp Glu Leu Leu Trp Ile         
        195                 200                 205                     

aat gac aca aca gct ccc act acg gtt ccg gcc ccc gct gcc gag cac      672
Asn Asp Thr Thr Ala Pro Thr Thr Val Pro Ala Pro Ala Ala Glu His         
    210                 215                 220                         

aag ccg cag gca caa gca ggg acg cag gat ctg gct cat gtt tcc tcg      720
Lys Pro Gln Ala Gln Ala Gly Thr Gln Asp Leu Ala His Val Ser Ser         
225                 230                 235                 240         

cca gta ctg gct acc gag cta gag cgc cag gac aag cac tgg ggc ggc      768
Pro Val Leu Ala Thr Glu Leu Glu Arg Gln Asp Lys His Trp Gly Gly         
                245                 250                 255             

cgt ggc ccg gac ttc gcg ccc aag gct agc aac ctg tgg agc act cgc      816
Arg Gly Pro Asp Phe Ala Pro Lys Ala Ser Asn Leu Trp Ser Thr Arg         
            260                 265                 270                 

ccc gag cga gtg cag gag ggc tcg acc gta ctg atc aac ggc cca cag      864
Pro Glu Arg Val Gln Glu Gly Ser Thr Val Leu Ile Asn Gly Pro Gln         
        275                 280                 285                     

ttt ggc tgg tac aac ccg gcc tac acc tat ggc att ggc ttg cat ggc      912
Phe Gly Trp Tyr Asn Pro Ala Tyr Thr Tyr Gly Ile Gly Leu His Gly         
    290                 295                 300                         

gcc ggc ttc gat gtg gtg ggt aat acg cct ttt gcc tat ccg atc gta      960
Ala Gly Phe Asp Val Val Gly Asn Thr Pro Phe Ala Tyr Pro Ile Val         
305                 310                 315                 320         

ctg ttt ggc acc aat agc gag att gcc tgg ggg gcg act gct ggc ccg     1008
Leu Phe Gly Thr Asn Ser Glu Ile Ala Trp Gly Ala Thr Ala Gly Pro         
                325                 330                 335             

caa gat gtg gtg gac ata tat cag gaa aaa ttg aac ccc tcg cgt gcc     1056
Gln Asp Val Val Asp Ile Tyr Gln Glu Lys Leu Asn Pro Ser Arg Ala         
            340                 345                 350                 

gat cag tac tgg ttc aac aat gcc tgg cgc acg atg gag cag cgc aag     1104
Asp Gln Tyr Trp Phe Asn Asn Ala Trp Arg Thr Met Glu Gln Arg Lys         
        355                 360                 365                     

gaa cgt atc cag gta cgc ggt cag gct gat cgg gaa atg acg atc tgg     1152
Glu Arg Ile Gln Val Arg Gly Gln Ala Asp Arg Glu Met Thr Ile Trp         
    370                 375                 380                         

cgc acc gtg cac ggc cct gtg atg cag ttt gat tac gat cag ggc gcg     1200
Arg Thr Val His Gly Pro Val Met Gln Phe Asp Tyr Asp Gln Gly Ala         
385                 390                 395                 400         

gcg tac agc aag aaa cgc agc tgg gat ggc tat gag gtg cag tcc ttg     1248
Ala Tyr Ser Lys Lys Arg Ser Trp Asp Gly Tyr Glu Val Gln Ser Leu         
                405                 410                 415             

cta gcc tgg ttg aac gtg gcc aag gcc cgc aac tgg acg gag ttt ctg     1296
Leu Ala Trp Leu Asn Val Ala Lys Ala Arg Asn Trp Thr Glu Phe Leu         
            420                 425                 430                 

gat caa gcc agc aag atg gcg att tcg atc aac tgg tac tac gcc gac     1344
Asp Gln Ala Ser Lys Met Ala Ile Ser Ile Asn Trp Tyr Tyr Ala Asp         
        435                 440                 445                     

aag cac ggc aat att ggt tat gtc tcg ccg gcc ttc ctg ccc cag cgt     1392
Lys His Gly Asn Ile Gly Tyr Val Ser Pro Ala Phe Leu Pro Gln Arg         
    450                 455                 460                         

cct gcc gat cag gac atc cgt gtc cct gcc aag ggg gat ggc agc atg     1440
Pro Ala Asp Gln Asp Ile Arg Val Pro Ala Lys Gly Asp Gly Ser Met         
465                 470                 475                 480         

gag tgg ctg ggc atc aag agt ttc gac gcg att ccc aaa gcc tac aat     1488
Glu Trp Leu Gly Ile Lys Ser Phe Asp Ala Ile Pro Lys Ala Tyr Asn         
                485                 490                 495             

cca ccc cag ggc tat ctg gtc aac tgg aac aac aag cct gcg ccg gac     1536
Pro Pro Gln Gly Tyr Leu Val Asn Trp Asn Asn Lys Pro Ala Pro Asp         
            500                 505                 510                 

aaa acc aat acg gat act tac tat tgg acc tat ggc gac cgc atg aat     1584
Lys Thr Asn Thr Asp Thr Tyr Tyr Trp Thr Tyr Gly Asp Arg Met Asn         
        515                 520                 525                     

gaa ctg gtc agt cag tac cag cag aaa gac ctc ttc agt gtg cag gag     1632
Glu Leu Val Ser Gln Tyr Gln Gln Lys Asp Leu Phe Ser Val Gln Glu         
    530                 535                 540                         

atc tgg gag ttc aat caa aaa gcc tcc tat agc gat gtg aac tgg cgc     1680
Ile Trp Glu Phe Asn Gln Lys Ala Ser Tyr Ser Asp Val Asn Trp Arg         
545                 550                 555                 560         

tac ttc cgc cca cat ctg gaa aag ctg gcg caa cag ctg ccg gcc gac     1728
Tyr Phe Arg Pro His Leu Glu Lys Leu Ala Gln Gln Leu Pro Ala Asp         
                565                 570                 575             

gat agc agc aag gcg gcg ctg acg atg ttg ctc gcc tgg gat gga atg     1776
Asp Ser Ser Lys Ala Ala Leu Thr Met Leu Leu Ala Trp Asp Gly Met         
            580                 585                 590                 

gaa cag gat cag gga ggg caa aat gcc gga ccg gcg cgg gtg ctc ttc     1824
Glu Gln Asp Gln Gly Gly Gln Asn Ala Gly Pro Ala Arg Val Leu Phe         
        595                 600                 605                     

aag acc tgg ctg gaa gaa atg tac aag cag gtc ttg atg ccg gtg gtg     1872
Lys Thr Trp Leu Glu Glu Met Tyr Lys Gln Val Leu Met Pro Val Val         
    610                 615                 620                         

cct gaa tcg cat cgc gcc atg tat agc cag act ggt ttt gcc acg cag     1920
Pro Glu Ser His Arg Ala Met Tyr Ser Gln Thr Gly Phe Ala Thr Gln         
625                 630                 635                 640         

caa ggt ccc aac ccc ggt tcc atc aac ttg agc atg ggc acc aag gtc     1968
Gln Gly Pro Asn Pro Gly Ser Ile Asn Leu Ser Met Gly Thr Lys Val         
                645                 650                 655             

ttg ttg cgt gcc ttg gtg ctg gaa gcc cat ccc gat ccc aag cgt gtg     2016
Leu Leu Arg Ala Leu Val Leu Glu Ala His Pro Asp Pro Lys Arg Val         
            660                 665                 670                 

aat gtc ttt ggt gag cgt tcg tct cag gaa atc atg cac aca gct ttg     2064
Asn Val Phe Gly Glu Arg Ser Ser Gln Glu Ile Met His Thr Ala Leu         
        675                 680                 685                     

caa aat gcg cag gcc cgc ttg agc cag gag cag ggc gct cag atg gcg     2112
Gln Asn Ala Gln Ala Arg Leu Ser Gln Glu Gln Gly Ala Gln Met Ala         
    690                 695                 700                         

cgc tgg acc atg ccg acc tcc gtg cat cgt ttc agc gac aag aac ttc     2160
Arg Trp Thr Met Pro Thr Ser Val His Arg Phe Ser Asp Lys Asn Phe         
705                 710                 715                 720         

acg gga acc ccg cag acg atg cct ggc aat acc ttt gcc ttt acc ggc     2208
Thr Gly Thr Pro Gln Thr Met Pro Gly Asn Thr Phe Ala Phe Thr Gly         
                725                 730                 735             

tat cag aat cga ggc acg gaa aat aac cgc gtg gtg ttt gat gcc aag     2256
Tyr Gln Asn Arg Gly Thr Glu Asn Asn Arg Val Val Phe Asp Ala Lys         
            740                 745                 750                 

ggc gtg gag ttc tgc gac gcc atg ccg ccc ggc caa agc ggt ttc acc     2304
Gly Val Glu Phe Cys Asp Ala Met Pro Pro Gly Gln Ser Gly Phe Thr         
        755                 760                 765                     

gac cgc aat gga gtg cgc agc ccg cat tat gag gat cag ctg aag ttg     2352
Asp Arg Asn Gly Val Arg Ser Pro His Tyr Glu Asp Gln Leu Lys Leu         
    770                 775                 780                         

tac gag aac ttc gag tgc aag acg atg gat gtg acg cat gcg gac att     2400
Tyr Glu Asn Phe Glu Cys Lys Thr Met Asp Val Thr His Ala Asp Ile         
785                 790                 795                 800         

cgt cgt aat gcg caa agc agc acg atg ctg ttg att cag cct cag cct     2448
Arg Arg Asn Ala Gln Ser Ser Thr Met Leu Leu Ile Gln Pro Gln Pro         
                805                 810                 815             


<210>  2
<211>  816
<212>  PRT
<213>  Alcaligenes faecalis

<400>  2

Met Gln Lys Gly Leu Val Arg Thr Gly Leu Val Ala Ala Gly Leu Ile 
1               5                   10                  15      


Leu Gly Trp Ala Gly Ala Pro Thr His Ala Gln Val Gln Ser Val Glu 
            20                  25                  30          


Val Met Arg Asp Ser Tyr Gly Val Pro His Val Phe Ala Asp Ser His 
        35                  40                  45              


Tyr Gly Leu Tyr Tyr Gly Tyr Gly Tyr Ala Val Ala Gln Asp Arg Leu 
    50                  55                  60                  


Phe Gln Met Asp Met Ala Arg Arg Ser Phe Val Gly Thr Thr Ala Ala 
65                  70                  75                  80  


Val Leu Gly Pro Gly Glu Gln Asp Ala Tyr Val Lys Tyr Asp Met Gln 
                85                  90                  95      


Val Arg Gln Asn Phe Thr Pro Ala Ser Ile Gln Arg Gln Ile Ala Ala 
            100                 105                 110         


Leu Ser Lys Asp Glu Arg Asp Ile Phe Arg Gly Tyr Ala Asp Gly Tyr 
        115                 120                 125             


Asn Ala Tyr Leu Glu Gln Val Arg Arg Arg Pro Glu Leu Leu Pro Lys 
    130                 135                 140                 


Glu Tyr Val Asp Phe Asp Phe Gln Pro Glu Pro Leu Thr Asp Phe Asp 
145                 150                 155                 160 


Val Val Met Ile Trp Val Gly Ser Met Ala Asn Arg Phe Ser Asp Thr 
                165                 170                 175     


Asn Leu Glu Val Thr Ala Leu Ala Met Arg Gln Ser Leu Glu Lys Gln 
            180                 185                 190         


His Gly Pro Glu Arg Gly Arg Ala Leu Phe Asp Glu Leu Leu Trp Ile 
        195                 200                 205             


Asn Asp Thr Thr Ala Pro Thr Thr Val Pro Ala Pro Ala Ala Glu His 
    210                 215                 220                 


Lys Pro Gln Ala Gln Ala Gly Thr Gln Asp Leu Ala His Val Ser Ser 
225                 230                 235                 240 


Pro Val Leu Ala Thr Glu Leu Glu Arg Gln Asp Lys His Trp Gly Gly 
                245                 250                 255     


Arg Gly Pro Asp Phe Ala Pro Lys Ala Ser Asn Leu Trp Ser Thr Arg 
            260                 265                 270         


Pro Glu Arg Val Gln Glu Gly Ser Thr Val Leu Ile Asn Gly Pro Gln 
        275                 280                 285             


Phe Gly Trp Tyr Asn Pro Ala Tyr Thr Tyr Gly Ile Gly Leu His Gly 
    290                 295                 300                 


Ala Gly Phe Asp Val Val Gly Asn Thr Pro Phe Ala Tyr Pro Ile Val 
305                 310                 315                 320 


Leu Phe Gly Thr Asn Ser Glu Ile Ala Trp Gly Ala Thr Ala Gly Pro 
                325                 330                 335     


Gln Asp Val Val Asp Ile Tyr Gln Glu Lys Leu Asn Pro Ser Arg Ala 
            340                 345                 350         


Asp Gln Tyr Trp Phe Asn Asn Ala Trp Arg Thr Met Glu Gln Arg Lys 
        355                 360                 365             


Glu Arg Ile Gln Val Arg Gly Gln Ala Asp Arg Glu Met Thr Ile Trp 
    370                 375                 380                 


Arg Thr Val His Gly Pro Val Met Gln Phe Asp Tyr Asp Gln Gly Ala 
385                 390                 395                 400 


Ala Tyr Ser Lys Lys Arg Ser Trp Asp Gly Tyr Glu Val Gln Ser Leu 
                405                 410                 415     


Leu Ala Trp Leu Asn Val Ala Lys Ala Arg Asn Trp Thr Glu Phe Leu 
            420                 425                 430         


Asp Gln Ala Ser Lys Met Ala Ile Ser Ile Asn Trp Tyr Tyr Ala Asp 
        435                 440                 445             


Lys His Gly Asn Ile Gly Tyr Val Ser Pro Ala Phe Leu Pro Gln Arg 
    450                 455                 460                 


Pro Ala Asp Gln Asp Ile Arg Val Pro Ala Lys Gly Asp Gly Ser Met 
465                 470                 475                 480 


Glu Trp Leu Gly Ile Lys Ser Phe Asp Ala Ile Pro Lys Ala Tyr Asn 
                485                 490                 495     


Pro Pro Gln Gly Tyr Leu Val Asn Trp Asn Asn Lys Pro Ala Pro Asp 
            500                 505                 510         


Lys Thr Asn Thr Asp Thr Tyr Tyr Trp Thr Tyr Gly Asp Arg Met Asn 
        515                 520                 525             


Glu Leu Val Ser Gln Tyr Gln Gln Lys Asp Leu Phe Ser Val Gln Glu 
    530                 535                 540                 


Ile Trp Glu Phe Asn Gln Lys Ala Ser Tyr Ser Asp Val Asn Trp Arg 
545                 550                 555                 560 


Tyr Phe Arg Pro His Leu Glu Lys Leu Ala Gln Gln Leu Pro Ala Asp 
                565                 570                 575     


Asp Ser Ser Lys Ala Ala Leu Thr Met Leu Leu Ala Trp Asp Gly Met 
            580                 585                 590         


Glu Gln Asp Gln Gly Gly Gln Asn Ala Gly Pro Ala Arg Val Leu Phe 
        595                 600                 605             


Lys Thr Trp Leu Glu Glu Met Tyr Lys Gln Val Leu Met Pro Val Val 
    610                 615                 620                 


Pro Glu Ser His Arg Ala Met Tyr Ser Gln Thr Gly Phe Ala Thr Gln 
625                 630                 635                 640 


Gln Gly Pro Asn Pro Gly Ser Ile Asn Leu Ser Met Gly Thr Lys Val 
                645                 650                 655     


Leu Leu Arg Ala Leu Val Leu Glu Ala His Pro Asp Pro Lys Arg Val 
            660                 665                 670         


Asn Val Phe Gly Glu Arg Ser Ser Gln Glu Ile Met His Thr Ala Leu 
        675                 680                 685             


Gln Asn Ala Gln Ala Arg Leu Ser Gln Glu Gln Gly Ala Gln Met Ala 
    690                 695                 700                 


Arg Trp Thr Met Pro Thr Ser Val His Arg Phe Ser Asp Lys Asn Phe 
705                 710                 715                 720 


Thr Gly Thr Pro Gln Thr Met Pro Gly Asn Thr Phe Ala Phe Thr Gly 
                725                 730                 735     


Tyr Gln Asn Arg Gly Thr Glu Asn Asn Arg Val Val Phe Asp Ala Lys 
            740                 745                 750         


Gly Val Glu Phe Cys Asp Ala Met Pro Pro Gly Gln Ser Gly Phe Thr 
        755                 760                 765             


Asp Arg Asn Gly Val Arg Ser Pro His Tyr Glu Asp Gln Leu Lys Leu 
    770                 775                 780                 


Tyr Glu Asn Phe Glu Cys Lys Thr Met Asp Val Thr His Ala Asp Ile 
785                 790                 795                 800 


Arg Arg Asn Ala Gln Ser Ser Thr Met Leu Leu Ile Gln Pro Gln Pro 
                805                 810                 815     


