                               SEQUENCE LISTING

<110> DAS, TAPAS
      MUKERJI, PRADIP
      PEREIRA, SUZETTE
      KRISHNAN, PADMAVATHY
 
<120> DELTA-8 DESATURASE GENES, ENZYMES ENCODED THEREBY AND USES 
      THEREOF

<130> 28072-139

<140>
<141>

<150> 61/103,107
<151> 2008-10-06

<160> 46    

<170> PatentIn version 3.5

<210> 1
<211> 419
<212> PRT
<213> Euglena gracialis

<400> 1
Met Lys Ser Lys Arg Gln Ala Leu Ser Pro Leu Gln Leu Met Glu Gln 
1               5                   10                  15      


Thr Tyr Asp Val Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile Glu 
            20                  25                  30          


Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val Met His Phe 
        35                  40                  45              


Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Pro Ser 
    50                  55                  60                  


Phe Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp Phe 
65                  70                  75                  80  


Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala Ser 
                85                  90                  95      


Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly Leu Gly Val 
            100                 105                 110         


Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe Ile Gly Ala 
        115                 120                 125             


Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu Ser His Asp 
    130                 135                 140                 


Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Leu Val 
145                 150                 155                 160 


Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Cys Trp 
                165                 170                 175     


Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly His 
            180                 185                 190         


Asp Pro Asp Ile Asp Asn Leu Pro Pro Leu Ala Trp Ser Glu Asp Asp 
        195                 200                 205             


Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln Gln 
    210                 215                 220                 


Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys Phe 
225                 230                 235                 240 


Gln Cys Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln Phe 
                245                 250                 255     


Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His Trp 
            260                 265                 270         


Thr Leu Lys Ala Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu Thr 
        275                 280                 285             


Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly Ile 
    290                 295                 300                 


Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly Asp 
305                 310                 315                 320 


Pro Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His Glu Thr 
                325                 330                 335     


Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly Leu 
            340                 345                 350         


Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn 
        355                 360                 365             


Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His Asn 
    370                 375                 380                 


Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu Leu 
385                 390                 395                 400 


Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln Pro Ala Gly 
                405                 410                 415     


Lys Ala Leu 
            


<210> 2
<211> 423
<212> PRT
<213> Pavlova lutheri

<400> 2
Met Gly Lys Gly Gly Asp Gly Gly Ala Gln Ala Val Ser Gly Thr Asp 
1               5                   10                  15      


Ala Ser Leu Ala Glu Val Ser Ser Val Asp Ser Lys Ser Val His Val 
            20                  25                  30          


Val Leu Tyr Gly Lys Arg Val Asp Val Thr Lys Phe Gln Lys Ala His 
        35                  40                  45              


Pro Gly Gly Ser Lys Val Phe Arg Ile Phe Gln Glu Arg Asp Ala Thr 
    50                  55                  60                  


Glu Gln Phe Glu Ser Tyr His Ser Pro Lys Ala Ile Lys Met Met Glu 
65                  70                  75                  80  


Gly Met Leu Lys Lys Ser Glu Asp Ala Pro Ala Ser Val Pro Leu Pro 
                85                  90                  95      


Ser Arg Ser Thr Met Gly Thr Glu Phe Lys Glu Met Ile Glu Arg His 
            100                 105                 110         


Lys Arg Ala Gly Leu Tyr Asp Pro Cys Pro Leu Asp Glu Leu Phe Lys 
        115                 120                 125             


Leu Thr Ile Val Leu Ala Pro Ile Phe Val Gly Ala Tyr Leu Val Arg 
    130                 135                 140                 


Ser Gly Val Ser Pro Leu Ala Gly Ala Leu Ser Met Gly Phe Gly Phe 
145                 150                 155                 160 


Tyr Leu Asp Gly Trp Leu Ala His Asp Tyr Leu His His Ala Val Phe 
                165                 170                 175     


Lys Gly Ser Val Asn Thr Leu Val Lys Ala Asn Asn Ala Met Gly Tyr 
            180                 185                 190         


Ala Leu Gly Phe Leu Gln Gly Tyr Asp Val Ala Trp Trp Arg Ala Arg 
        195                 200                 205             


His Asn Thr His His Val Cys Thr Asn Glu Asp Gly Ser Asp Pro Asp 
    210                 215                 220                 


Ile Lys Thr Ala Pro Leu Leu Ile Tyr Val Arg Glu Asn Pro Ser Ile 
225                 230                 235                 240 


Ala Lys Arg Leu Asn Phe Phe Gln Arg Trp Gln Gln Tyr Tyr Tyr Val 
                245                 250                 255     


Pro Thr Met Ala Ile Leu Asp Leu Tyr Trp Arg Leu Glu Ser Ile Ala 
            260                 265                 270         


Tyr Val Ala Val Arg Leu Pro Lys Met Trp Met Gln Ala Ala Ala Leu 
        275                 280                 285             


Ala Ala His Tyr Ala Leu Leu Cys Trp Val Phe Ala Ala His Leu Asn 
    290                 295                 300                 


Leu Ile Pro Leu Met Met Val Ala Arg Gly Phe Ala Thr Gly Ile Val 
305                 310                 315                 320 


Val Phe Ala Thr His Tyr Gly Glu Asp Ile Leu Asp Arg Glu His Val 
                325                 330                 335     


Glu Gly Met Thr Leu Val Glu Gln Thr Ala Lys Thr Ser Arg Asn Ile 
            340                 345                 350         


Thr Gly Gly Trp Leu Val Asn Val Leu Thr Gly Phe Ile Ser Leu Gln 
        355                 360                 365             


Thr Glu His His Leu Phe Pro Met Met Pro Thr Gly Asn Leu Met Thr 
    370                 375                 380                 


Ile Gln Pro Glu Val Arg Asp Phe Phe Lys Lys His Gly Leu Glu Tyr 
385                 390                 395                 400 


Arg Glu Gly Asn Leu Phe Gln Cys Val His Gln Asn Ile Lys Ala Leu 
                405                 410                 415     


Ala Phe Glu His Leu Leu His 
            420             


<210> 3
<211> 427
<212> PRT
<213> Pavlova salina

<400> 3
Met Gly Arg Gly Gly Asp Ser Ser Gly Gln Ala His Pro Ala Ala Glu 
1               5                   10                  15      


Leu Ala Val Pro Ser Asp Arg Ala Glu Val Ser Asn Ala Asp Ser Lys 
            20                  25                  30          


Ala Leu His Ile Val Leu Tyr Gly Lys Arg Val Asp Val Thr Lys Phe 
        35                  40                  45              


Gln Arg Thr His Pro Gly Gly Ser Lys Val Phe Arg Ile Phe Gln Asp 
    50                  55                  60                  


Arg Asp Ala Thr Glu Gln Phe Glu Ser Tyr His Ser Lys Arg Ala Ile 
65                  70                  75                  80  


Lys Met Met Glu Gly Met Leu Lys Lys Ser Glu Asp Ala Pro Ala Asp 
                85                  90                  95      


Thr Pro Leu Pro Ser Gln Ser Pro Met Gly Lys Asp Phe Lys Ala Met 
            100                 105                 110         


Ile Glu Arg His Val Ala Ala Gly Tyr Tyr Asp Pro Cys Pro Leu Asp 
        115                 120                 125             


Glu Leu Phe Lys Leu Ser Leu Val Leu Leu Pro Thr Phe Ala Gly Met 
    130                 135                 140                 


Tyr Met Leu Lys Ala Gly Val Gly Ser Pro Leu Cys Gly Ala Leu Met 
145                 150                 155                 160 


Val Ser Phe Gly Trp Tyr Leu Asp Gly Trp Leu Ala His Asp Tyr Leu 
                165                 170                 175     


His His Ser Val Phe Lys Gly Ser Val Ala Arg Thr Val Gly Trp Asn 
            180                 185                 190         


Asn Ala Ala Gly Tyr Phe Leu Gly Phe Val Gln Gly Tyr Ala Val Glu 
        195                 200                 205             


Trp Trp Arg Ala Arg His Asn Thr His His Val Cys Thr Asn Glu Asp 
    210                 215                 220                 


Gly Ser Asp Pro Asp Ile Lys Thr Ala Pro Leu Leu Ile Tyr Val Arg 
225                 230                 235                 240 


Asn Lys Pro Ser Ile Ala Lys Arg Leu Asn Ala Phe Gln Arg Tyr Gln 
                245                 250                 255     


Gln Tyr Tyr Tyr Val Pro Val Met Ala Ile Leu Asp Leu Tyr Trp Arg 
            260                 265                 270         


Leu Glu Ser Ile Ala Tyr Val Ala Met Arg Leu Pro Lys Met Leu Pro 
        275                 280                 285             


Gln Ala Leu Ala Leu Val Ala His Tyr Ala Ile Val Ala Trp Val Phe 
    290                 295                 300                 


Ala Gly Asn Tyr His Leu Leu Pro Leu Val Thr Val Leu Arg Gly Phe 
305                 310                 315                 320 


Gly Thr Gly Ile Thr Val Phe Ala Thr His Tyr Gly Glu Asp Ile Leu 
                325                 330                 335     


Asp Ala Asp Gln Val Arg His Met Thr Leu Val Glu Gln Thr Ala Leu 
            340                 345                 350         


Thr Ser Arg Asn Ile Ser Gly Gly Trp Leu Val Asn Val Leu Thr Gly 
        355                 360                 365             


Phe Ile Ser Leu Gln Thr Glu His His Leu Phe Pro Met Met Pro Thr 
    370                 375                 380                 


Gly Asn Leu Met Thr Ile Gln Pro Glu Val Arg Ala Phe Phe Lys Lys 
385                 390                 395                 400 


His Gly Leu Glu Tyr Arg Glu Gly Asn Leu Ile Glu Cys Val Arg Gln 
                405                 410                 415     


Asn Ile Arg Ala Leu Ala Phe Glu His Leu Leu 
            420                 425         


<210> 4
<211> 457
<212> PRT
<213> Perkinsus marinus

<400> 4
Met Thr Thr Ser Thr Thr Thr Val Gln Leu Gln Glu Asp Leu Ser Ser 
1               5                   10                  15      


Gly Asp Gln Asn Ala His Pro Ser Pro Ser Arg Ala Thr Pro Ser Val 
            20                  25                  30          


Gly Asp Thr Lys Glu Asp Ala Arg Val Val Ile Lys Leu Phe Gly Thr 
        35                  40                  45              


Trp Val Asp Val Thr Ala Trp Leu Asn Asp His Pro Gly Gly Ser Lys 
    50                  55                  60                  


Val Leu Arg Ala Phe Asn Lys Lys Asp Ala Thr Asp Ala Val Met Ala 
65                  70                  75                  80  


Met His Thr Asp Glu Ala Ile Lys Arg Ile Ile Arg Phe Ser Asn Val 
                85                  90                  95      


Val Ser Ser Ala Pro Ile Asn Ala Ser Ile Gly Asp Val Gln Val Ile 
            100                 105                 110         


Glu Lys Ser Leu Ser Arg Glu Gln Leu Met Tyr Tyr Lys Leu Arg Thr 
        115                 120                 125             


Leu Ala Arg Asn Gln Gly Trp Phe Gln Ser Asn Leu Leu Tyr Glu Gly 
    130                 135                 140                 


Val Lys Ala Met Ile Ala Phe Gly Leu Leu Ile Ile Gly Phe Ala Thr 
145                 150                 155                 160 


Leu Tyr Phe Asp Tyr Gly Ile Trp Ser Thr Ala Leu Ile Gly Phe Ala 
                165                 170                 175     


Trp Phe Gln Leu Gly Trp Leu Gly His Asp Trp Ser His His Thr Ala 
            180                 185                 190         


Leu Pro Lys Ser Thr Thr Asn Cys Ala Asn Tyr Asn Asp Tyr Leu Gly 
        195                 200                 205             


Trp Leu Thr Gly Leu Ala Arg Gly Asn Thr Leu Leu Trp Trp Lys Leu 
    210                 215                 220                 


Arg His Asn Thr His His Val Leu Thr Asn Gln Tyr Glu Asn Asp Pro 
225                 230                 235                 240 


Asp Ile Leu Thr Gln Pro Pro Leu His Phe Phe Glu Asp Phe Asp Val 
                245                 250                 255     


Gly Asn Val Asn Arg Tyr Gln Ala Val Tyr Tyr Leu Pro Met Leu Thr 
            260                 265                 270         


Leu Leu His Leu Phe Trp Leu Tyr Glu Ser Val Leu Val Cys Leu Arg 
        275                 280                 285             


Gln Ser Lys Ser Ile Asn Arg Tyr Asn Arg Met His Ala Arg Arg Asp 
    290                 295                 300                 


Thr Val Ala Leu Val Leu His Ile Leu Ile Val Gly Ile Ile Ser Tyr 
305                 310                 315                 320 


Thr Ser Gly Lys Tyr Leu Leu Ile Leu Leu Ala Tyr Met Leu Ser Gly 
                325                 330                 335     


Phe Leu Thr Ala Val Val Val Phe Ala Ser His Tyr Asn Glu Pro Arg 
            340                 345                 350         


Val Ala Ser Gly Glu Ser Leu Ser Leu Val Arg Gln Thr Leu Leu Thr 
        355                 360                 365             


Thr Ile Asn Ile Gly Ser Phe Ser Asp Thr His Trp Glu Lys Lys Leu 
    370                 375                 380                 


Trp Phe Tyr Leu Thr Gly Gly Leu Asn Met Gln Ile Glu His His Leu 
385                 390                 395                 400 


Phe Pro Thr Met Pro Arg His Asn Leu Pro Lys Thr Thr Phe Leu Val 
                405                 410                 415     


Lys Ser Leu Ala Gln Glu Leu Gly Leu Pro Tyr Lys Glu Thr Asn Ile 
            420                 425                 430         


Val Ser Leu Thr Lys Ala Ala Val Thr Thr Leu His His Asn Ala Leu 
        435                 440                 445             


Arg Asn Ile Glu Arg Leu Leu Ala Arg 
    450                 455         


<210> 5
<211> 456
<212> PRT
<213> Acanthamoenba castellani

<400> 5
Met Val Leu Thr Thr Pro Ala Leu Asn Leu Lys Lys Glu Arg Thr Ser 
1               5                   10                  15      


Phe Thr Gln Glu Ile Ser Lys Leu Trp Val Leu His Gly Gln Val Tyr 
            20                  25                  30          


Asp Phe Thr Asp Phe Val Lys Tyr His Pro Ala Gly Ser Arg Ala Ile 
        35                  40                  45              


Leu Leu Gly Arg Gly Arg Asp Cys Thr Val Leu Phe Glu Ser Tyr His 
    50                  55                  60                  


Thr Val Leu Pro Ser Asp Ala Leu Asp Glu Lys Tyr Arg Val Ser Ala 
65                  70                  75                  80  


Pro Asn Ala Lys Leu Glu Glu Ser Arg Ser Ala Lys Leu Phe Ser Phe 
                85                  90                  95      


Glu Glu Gly Ser Phe Tyr Arg Thr Leu Lys Gln Arg Thr Arg Glu Tyr 
            100                 105                 110         


Phe Lys Thr Asn Asn Leu Ser Thr Lys Ala Thr Thr Met Glu Val Ile 
        115                 120                 125             


Tyr Phe Val Ala Thr Ile Leu Ser Ile Tyr Phe Cys Thr Trp Ala Ala 
    130                 135                 140                 


Phe Val Gln Gly Ser Leu Ile Ala Ala Val Leu His Gly Val Gly Arg 
145                 150                 155                 160 


Ala Ile Cys Ile Ile Gln Pro Ile His Ala Thr Ser His Tyr Ala Met 
                165                 170                 175     


Phe Arg Ser Val Trp Leu Asn Gln Trp Ala Tyr Arg Ile Ser Met Ala 
            180                 185                 190         


Val Ser Gly Ser Ser Pro Ala Gln Trp Thr Thr Lys His Val Ile Asn 
        195                 200                 205             


His His Val Glu Thr Asn Leu Cys Pro Thr Asp Asp Asp Thr Asn Tyr 
    210                 215                 220                 


Pro Ile Lys Arg Ile Leu His Glu Phe Pro Arg Ile Phe Phe His Lys 
225                 230                 235                 240 


Tyr Gln His Ile Tyr Ile Trp Leu Val Tyr Pro Tyr Thr Thr Ile Leu 
                245                 250                 255     


Trp His Phe Ser Asn Leu Ala Lys Leu Ala Leu Gly Ala Ala Arg Gly 
            260                 265                 270         


Gln Met Tyr Glu Gly Ile Ala Lys Val Ser Gln Glu Thr Ser Gly Asp 
        275                 280                 285             


Trp Val Glu Thr Ala Met Thr Leu Phe Phe Phe Thr Phe Ser Arg Leu 
    290                 295                 300                 


Leu Leu Pro Phe Leu Cys Leu Pro Phe Thr Thr Ala Ala Ala Val Phe 
305                 310                 315                 320 


Leu Leu Ser Glu Trp Thr Cys Ser Thr Trp Phe Ala Ile Gln Phe Ala 
                325                 330                 335     


Val Ser His Glu Val Asp Glu Cys Val Glu His Glu Lys Ser Val Leu 
            340                 345                 350         


Asp Thr Ile Lys Ala Asn Glu Ala Lys Gly Ile Val Asn Gln Gly Gly 
        355                 360                 365             


Leu Val Asp Trp Gly Ala His Gln Val Arg Ala Ser His Asn Tyr Ser 
    370                 375                 380                 


Ala Asp Ser Leu Leu Ser Leu His Phe Ser Gly Gly Leu Asn Leu Gln 
385                 390                 395                 400 


Ile Glu His His Leu Phe Pro Ser Val His Tyr Thr His Tyr Pro Ala 
                405                 410                 415     


Pro Ser Lys Leu Val Gln Gln Thr Cys Lys Glu Phe Asn Leu Pro Cys 
            420                 425                 430         


Thr Leu Ser Pro Ser Met Met Gly Ala Val Thr Lys His Tyr His Gln 
        435                 440                 445             


Leu Lys Lys Met Gly Ala Glu Asn 
    450                 455     


<210> 6
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 6
cgcgacgcga cggassmgtt crwgkykwws cac                                    33


<210> 7
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 7
ggctggcttk ckcacgacww cyygcatcac                                        30


<210> 8
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 8
tggmrssygc gccataacrc gcaccacgtg kscagcaac                              39


<210> 9
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 9
atagtgggtt gcaaagacaa csayssccgt cscgaa                                 36


<210> 10
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 10
gggcatsrtg gggaagaggt gatgctcgrt ctg                                    33


<210> 11
<211> 531
<212> DNA
<213> Emiliana huxleyi

<400> 11
gccctttgga gggtgcgcca taacgcgcac cacgtgggca gcaacgaaga aggcaacgac       60

cccgacatca tgaccgcgcc tgtgctcatc ttcgtgcgca acagcccggt gatcgccgct      120

gccctcaacg cggcgcagcg gtggcagcag tactactacg tgcccgcgat gagcctcatg      180

gacatgtact ggcgcttcga gtcgatgcag tacctggccg cgcggccctt caacaaggtc      240

tgggcctcgt gggcgctcct cgcgctgcac tactcctttg tcggctacat gttccacgga      300

cagtaccagt ggctgctgct gacgatgctg gtgcgcggct tcctcacggg catcgtcgtc      360

ttctcgacgc attatggcga ggaggtcatc ccgggcgacc acggcatgac actcgtcgag      420

cagacggcgc tcacctctcg caacatcacc ggcgggtacc tcgtcaacct gctcacgggc      480

tacatctcgc tgcagaccga gcatcacctc ttccccatga tgcccaaggg c               531


<210> 12
<211> 177
<212> PRT
<213> Emiliana huxleyi

<400> 12
Ala Leu Trp Arg Val Arg His Asn Ala His His Val Gly Ser Asn Glu 
1               5                   10                  15      


Glu Gly Asn Asp Pro Asp Ile Met Thr Ala Pro Val Leu Ile Phe Val 
            20                  25                  30          


Arg Asn Ser Pro Val Ile Ala Ala Ala Leu Asn Ala Ala Gln Arg Trp 
        35                  40                  45              


Gln Gln Tyr Tyr Tyr Val Pro Ala Met Ser Leu Met Asp Met Tyr Trp 
    50                  55                  60                  


Arg Phe Glu Ser Met Gln Tyr Leu Ala Ala Arg Pro Phe Asn Lys Val 
65                  70                  75                  80  


Trp Ala Ser Trp Ala Leu Leu Ala Leu His Tyr Ser Phe Val Gly Tyr 
                85                  90                  95      


Met Phe His Gly Gln Tyr Gln Trp Leu Leu Leu Thr Met Leu Val Arg 
            100                 105                 110         


Gly Phe Leu Thr Gly Ile Val Val Phe Ser Thr His Tyr Gly Glu Glu 
        115                 120                 125             


Val Ile Pro Gly Asp His Gly Met Thr Leu Val Glu Gln Thr Ala Leu 
    130                 135                 140                 


Thr Ser Arg Asn Ile Thr Gly Gly Tyr Leu Val Asn Leu Leu Thr Gly 
145                 150                 155                 160 


Tyr Ile Ser Leu Gln Thr Glu His His Leu Phe Pro Met Met Pro Lys 
                165                 170                 175     


Gly 
    


<210> 13
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 13
gatcaccggg ctgttgcgca cgaag                                             25


<210> 14
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 14
agcggataac aatttcacac aggaaacagc                                        30


<210> 15
<211> 672
<212> DNA
<213> Emiliana huxleyi


<220>
<221> modified_base
<222> (572)..(573)
<223> a, c, g, or t

<400> 15
atgggcaagg gcggcaacgc gaacccgcgg gagctcaaag gcggcaaggc cgagcagctg       60

acagtctacc tgtatggcaa ggctgtcgac gtctcgaagt tcgcgaagct gcacccggga      120

ggcgccaagg cgctgcgcat cttcaacaac cgtgacgcca ccgagcagtt cgagatgtac      180

cactcgcccg ccgcccacaa gatgatgcgt gcgatgtcga agagcgcgcc ggaggccccg      240

agggagagcg aggtcgcgac gtcggtcgtt gggacggact tcgccaagct gacgcagacg      300

ctgcacgacg tcggatgctt cgaccctcac tacccagacg aggccttcaa gctcggcctc      360

acgctgctgc ccggattcct cggcttctac ctgctgcgga gcggcatgcc ggcgctcgga      420

tccttcctga tcgctttctc gtactacatg tctgggtgga cctcccacga ttacttgcac      480

cacggctgcc tcaagggcgg ccaaaagcag ctggtgcact ggaacaacgc cgtcggctac      540

gcaatcggcg cttggcaggg ctacgcggtc gnntggtggc gagcgcgcca caacacgcac      600

cacctcgtca cgaacgaaga aggcaacgac cccgacatca tgaccgcgcc tgtgctcatc      660

ttcgtgcgca ac                                                          672


<210> 16
<211> 224
<212> PRT
<213> Emiliana huxleyi


<220>
<221> MOD_RES
<222> (191)..(191)
<223> Any amino acid

<400> 16
Met Gly Lys Gly Gly Asn Ala Asn Pro Arg Glu Leu Lys Gly Gly Lys 
1               5                   10                  15      


Ala Glu Gln Leu Thr Val Tyr Leu Tyr Gly Lys Ala Val Asp Val Ser 
            20                  25                  30          


Lys Phe Ala Lys Leu His Pro Gly Gly Ala Lys Ala Leu Arg Ile Phe 
        35                  40                  45              


Asn Asn Arg Asp Ala Thr Glu Gln Phe Glu Met Tyr His Ser Pro Ala 
    50                  55                  60                  


Ala His Lys Met Met Arg Ala Met Ser Lys Ser Ala Pro Glu Ala Pro 
65                  70                  75                  80  


Arg Glu Ser Glu Val Ala Thr Ser Val Val Gly Thr Asp Phe Ala Lys 
                85                  90                  95      


Leu Thr Gln Thr Leu His Asp Val Gly Cys Phe Asp Pro His Tyr Pro 
            100                 105                 110         


Asp Glu Ala Phe Lys Leu Gly Leu Thr Leu Leu Pro Gly Phe Leu Gly 
        115                 120                 125             


Phe Tyr Leu Leu Arg Ser Gly Met Pro Ala Leu Gly Ser Phe Leu Ile 
    130                 135                 140                 


Ala Phe Ser Tyr Tyr Met Ser Gly Trp Thr Ser His Asp Tyr Leu His 
145                 150                 155                 160 


His Gly Cys Leu Lys Gly Gly Gln Lys Gln Leu Val His Trp Asn Asn 
                165                 170                 175     


Ala Val Gly Tyr Ala Ile Gly Ala Trp Gln Gly Tyr Ala Val Xaa Trp 
            180                 185                 190         


Trp Arg Ala Arg His Asn Thr His His Leu Val Thr Asn Glu Glu Gly 
        195                 200                 205             


Asn Asp Pro Asp Ile Met Thr Ala Pro Val Leu Ile Phe Val Arg Asn 
    210                 215                 220                 


<210> 17
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 17
gtaccagtgg ctgctgctga cgatg                                             25


<210> 18
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 18
ctggcgcttc gagtcgatgc agtacct                                           27


<210> 19
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 19
cttcgtgcgc aacagcccgg tgatc                                             25


<210> 20
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 20
cccagtcacg acgttgtaaa acgacggcca g                                      31


<210> 21
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 21
gctgtcaacg atacgctacg taacggcatg acagtgt                                37


<210> 22
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 22
gctgtcaacg atacgctacg taacg                                             25


<210> 23
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 23
cgctacgtaa cggcatgaca gtg                                               23


<210> 24
<211> 589
<212> DNA
<213> Emiliana huxleyi

<400> 24
gtaccagtgg ctgctgctga cgatgctggt gcgcggcttc ctcacgggca tcgtcgtctt       60

ctcgacgcat tatggcgagg aggtcatccc gggcgaccac ggcatgacac tcgtcgagca      120

gacggcgctc acctctcgca acatcaccgg cgggtacctc gtcaacctgc tcacgggcta      180

catctcgctg cagacggagc accacctctg gccgatgatg cccaccgcgc gcctcgaggc      240

ggcgcagccc tacgcgcgcg ccttcttcaa gaagcacggc ttcgtctacc gcgagtcgaa      300

cctcgtcgag tgcgtcaagt acaacatcgc cgccctcgac atcaccacgc gcaacggcga      360

gtgggcagag atgccgcact agccggcggg ctccgcctct ccaggccgga cgtgaccgcg      420

ccgcgggcga cagcgaccgc gcgggccgca cgggccacct gttgagggga gcggcgtcgc      480

aacaggcaga gagataataa tagagaggca tgtgtgctgg gacgctattg gctatttcgg      540

tatatggaca ggcaggtagc gcgcaaaaaa aaaaaaaaaa aaaaaaaaa                  589


<210> 25
<211> 126
<212> PRT
<213> Emiliana huxleyi

<400> 25
Tyr Gln Trp Leu Leu Leu Thr Met Leu Val Arg Gly Phe Leu Thr Gly 
1               5                   10                  15      


Ile Val Val Phe Ser Thr His Tyr Gly Glu Glu Val Ile Pro Gly Asp 
            20                  25                  30          


His Gly Met Thr Leu Val Glu Gln Thr Ala Leu Thr Ser Arg Asn Ile 
        35                  40                  45              


Thr Gly Gly Tyr Leu Val Asn Leu Leu Thr Gly Tyr Ile Ser Leu Gln 
    50                  55                  60                  


Thr Glu His His Leu Trp Pro Met Met Pro Thr Ala Arg Leu Glu Ala 
65                  70                  75                  80  


Ala Gln Pro Tyr Ala Arg Ala Phe Phe Lys Lys His Gly Phe Val Tyr 
                85                  90                  95      


Arg Glu Ser Asn Leu Val Glu Cys Val Lys Tyr Asn Ile Ala Ala Leu 
            100                 105                 110         


Asp Ile Thr Thr Arg Asn Gly Glu Trp Ala Glu Met Pro His 
        115                 120                 125     


<210> 26
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 26
aaagaattca tgggcaaggg cggcaacgcg aacc                                   34


<210> 27
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 27
aaaaagcttc tagtgcggca tctctgccca ctcg                                   34


<210> 28
<211> 1254
<212> DNA
<213> Emiliana huxleyi

<400> 28
atgggcaagg gcggcaacgc gaacccgcgg gagctcaaag gcggcaaggc cgagcagctg       60

acagtctacc tgcatggcaa ggctgtcgac gtctcgaagt tcgcgaagct gcacccggga      120

ggcgccaagg cgctgcgcat cttcaacaac cgtgacgcca ccgagcagtt cgagatgtac      180

cactcgcccg ccgcccacaa gatgatgcgt gcgatgtcga agagcgcgcc ggaggccccg      240

agggagagcg aggtcgcgac gtcggtcgtt gggacggact tcgccaagct gacgcagacg      300

ctgcacgacg tcggatgctt cgaccctcac tacccagacg aggccttcaa gctcggcctc      360

acgctgctgc ccggattcct cggcttctac ctgctgcgga gcggcatgcc ggcgctcgga      420

tccttcctga tcgctttctc gtactacatg tctgggtgga cctcccacga ttacttgcac      480

cacggctgcc tcaagggcgg ccaaaagcag ctggtgcact ggaacaacgc cgtcggctac      540

gcaatcggcg cttggcaggg ctacgcggtc ggctggtggc gagcgcgcca caacacgcac      600

cacctcgtca cgaacgaaga aggcaacgac cccgacatca tgaccgcgcc tgtgctcatc      660

ttcgtgcgca acaacccggt gatcgccgct gccctcaacg cggcgcagcg gtggcagcag      720

tactactacg tgcccgcgat gagcctcatg gacatgtact ggcgcttcga gtcgatgcag      780

tacctggccg cgcggccctt caacaaggtc tgggcctcgt gggcgctcct cgcgctgcac      840

tactcctttg tcggctacat gttccacgga cagtaccagt ggctgctgct gacgatgctg      900

gtgcgcggct tcctcacggg catcgtcgtc ttctcgacgc attatggcga ggaggtcatc      960

ccgggcgacc acggcatgac actcgtcgag cagacggcgc acacctctcg caacatcacc     1020

ggcgggtacc tcgtcaacct gctcacgggc tacatctcgc tgcagacgga gcaccacctc     1080

tggccgatga tgcccaccgc gcgcctcgag gcggcgcagc cctacgcgcg cgccttcttc     1140

aagaagcacg gcttcgtcta ccgcgagtcg aacctcgtcg agtgcgtcaa gtacaacatc     1200

gccgccctcg acatcaccac gcgcaacggc gagtgggcag agatgccgca ctag           1254


<210> 29
<211> 417
<212> PRT
<213> Emiliana huxleyi

<400> 29
Met Gly Lys Gly Gly Asn Ala Asn Pro Arg Glu Leu Lys Gly Gly Lys 
1               5                   10                  15      


Ala Glu Gln Leu Thr Val Tyr Leu His Gly Lys Ala Val Asp Val Ser 
            20                  25                  30          


Lys Phe Ala Lys Leu His Pro Gly Gly Ala Lys Ala Leu Arg Ile Phe 
        35                  40                  45              


Asn Asn Arg Asp Ala Thr Glu Gln Phe Glu Met Tyr His Ser Pro Ala 
    50                  55                  60                  


Ala His Lys Met Met Arg Ala Met Ser Lys Ser Ala Pro Glu Ala Pro 
65                  70                  75                  80  


Arg Glu Ser Glu Val Ala Thr Ser Val Val Gly Thr Asp Phe Ala Lys 
                85                  90                  95      


Leu Thr Gln Thr Leu His Asp Val Gly Cys Phe Asp Pro His Tyr Pro 
            100                 105                 110         


Asp Glu Ala Phe Lys Leu Gly Leu Thr Leu Leu Pro Gly Phe Leu Gly 
        115                 120                 125             


Phe Tyr Leu Leu Arg Ser Gly Met Pro Ala Leu Gly Ser Phe Leu Ile 
    130                 135                 140                 


Ala Phe Ser Tyr Tyr Met Ser Gly Trp Thr Ser His Asp Tyr Leu His 
145                 150                 155                 160 


His Gly Cys Leu Lys Gly Gly Gln Lys Gln Leu Val His Trp Asn Asn 
                165                 170                 175     


Ala Val Gly Tyr Ala Ile Gly Ala Trp Gln Gly Tyr Ala Val Gly Trp 
            180                 185                 190         


Trp Arg Ala Arg His Asn Thr His His Leu Val Thr Asn Glu Glu Gly 
        195                 200                 205             


Asn Asp Pro Asp Ile Met Thr Ala Pro Val Leu Ile Phe Val Arg Asn 
    210                 215                 220                 


Asn Pro Val Ile Ala Ala Ala Leu Asn Ala Ala Gln Arg Trp Gln Gln 
225                 230                 235                 240 


Tyr Tyr Tyr Val Pro Ala Met Ser Leu Met Asp Met Tyr Trp Arg Phe 
                245                 250                 255     


Glu Ser Met Gln Tyr Leu Ala Ala Arg Pro Phe Asn Lys Val Trp Ala 
            260                 265                 270         


Ser Trp Ala Leu Leu Ala Leu His Tyr Ser Phe Val Gly Tyr Met Phe 
        275                 280                 285             


His Gly Gln Tyr Gln Trp Leu Leu Leu Thr Met Leu Val Arg Gly Phe 
    290                 295                 300                 


Leu Thr Gly Ile Val Val Phe Ser Thr His Tyr Gly Glu Glu Val Ile 
305                 310                 315                 320 


Pro Gly Asp His Gly Met Thr Leu Val Glu Gln Thr Ala His Thr Ser 
                325                 330                 335     


Arg Asn Ile Thr Gly Gly Tyr Leu Val Asn Leu Leu Thr Gly Tyr Ile 
            340                 345                 350         


Ser Leu Gln Thr Glu His His Leu Trp Pro Met Met Pro Thr Ala Arg 
        355                 360                 365             


Leu Glu Ala Ala Gln Pro Tyr Ala Arg Ala Phe Phe Lys Lys His Gly 
    370                 375                 380                 


Phe Val Tyr Arg Glu Ser Asn Leu Val Glu Cys Val Lys Tyr Asn Ile 
385                 390                 395                 400 


Ala Ala Leu Asp Ile Thr Thr Arg Asn Gly Glu Trp Ala Glu Met Pro 
                405                 410                 415     


His 
    


<210> 30
<211> 1257
<212> DNA
<213> Emiliana huxleyi

<400> 30
aaaatgggta aaggtggtaa tgctaatcca agagaattga aaggtggtaa agctgaacaa       60

ttgactgttt atttgcatgg taaagctgtt gatgtttcta aatttgctaa attgcatcca      120

ggtggtgcta aagcattgag aatttttaat aatagagatg ctactgaaca atttgaaatg      180

tatcattctc cagctgctca taaaatgatg agagctatgt ctaaatctgc tccagaagct      240

ccaagagaat ctgaagttgc tacttctgtt gttggtactg attttgctaa attgactcaa      300

actttgcatg atgttggttg ttttgatcca cattatccag atgaagcatt taaattgggt      360

ttgactttgt tgccaggttt tttgggtttt tatttgttga gatctggtat gccagctttg      420

ggttcttttt tgattgcttt ttcttattat atgtctggtt ggacttctca tgattatttg      480

catcatggtt gtttgaaagg tggtcaaaaa caattggttc attggaataa tgctgttggt      540

tatgctattg gtgcttggca aggttatgct gttggttggt ggagagctag acataatact      600

catcatttgg ttactaatga agaaggtaat gatccagata ttatgactgc tccagttttg      660

atttttgtta gaaataatcc agttattgct gctgctttga atgctgctca aagatggcaa      720

caatattatt atgttccagc tatgtctttg atggatatgt attggagatt tgaatctatg      780

caatatttgg ctgctagacc atttaataaa gtttgggctt cttgggcttt gttggctttg      840

cattattctt ttgttggtta tatgtttcat ggtcaatatc aatggttgtt gttgactatg      900

ttggttagag gttttttgac tggtattgtt gttttttcta ctcattatgg tgaagaagtt      960

attccaggtg atcatggtat gactttggtt gaacaaactg ctcatacttc tagaaatatt     1020

actggtggtt atttggttaa tttgttgact ggttatattt ctttgcaaac tgaacatcat     1080

ttgtggccaa tgatgccaac tgctagattg gaagctgctc aaccatatgc tagagctttt     1140

tttaaaaaac atggttttgt ttatagagaa tctaatttgg ttgaatgtgt taaatataat     1200

attgctgctt tggatattac tactagaaat ggtgaatggg ctgaaatgcc acattaa        1257


<210> 31
<211> 792
<212> DNA
<213> Isochrysis galbana

<400> 31
atggccctcg caaacgacgc gggagagcgc atctgggcgg ctgtgaccga cccggaaatc       60

ctcattggca ccttctcgta cttgctactc aaaccgctgc tccgcaattc cgggctggtg      120

gatgagaaga agggcgcata caggacgtcc atgatctggt acaacgttct gctggcgctc      180

ttctctgcgc tgagcttcta cgtgacggcg accgccctcg gctgggacta tggtacgggc      240

gcgtggctgc gcaggcaaac cggcgacaca ccgcagccgc tcttccagtg cccgtccccg      300

gtttgggact cgaagctctt cacatggacc gccaaggcat tctattactc caagtacgtg      360

gagtacctcg acacggcctg gctggtgctc aagggcaaga gggtctcctt tctccaggcc      420

ttccaccact ttggcgcgcc gtgggatgtg tacctcggca ttcggctgca caacgagggc      480

gtatggatct tcatgttttt caactcgttc attcacacca tcatgtacac ctactacggc      540

ctcaccgccg ccgggtataa gttcaaggcc aagccgctca tcaccgcgat gcagatctgc      600

cagttcgtgg gcggcttcct gttggtctgg gactacatca acgtcccctg cttcaactcg      660

gacaaaggga agttgttcag ctgggctttc aactatgcat acgtcggctc ggtcttcttg      720

ctcttctgcc actttttcta ccaggacaac ttggcaacga agaaatcggc caaggcgggc      780

aagcagctct ag                                                          792


<210> 32
<211> 5
<212> PRT
<213> Emiliana huxleyi

<400> 32
His Asp Tyr Leu His 
1               5   


<210> 33
<211> 5
<212> PRT
<213> Emiliana huxleyi

<400> 33
His Asn Thr His His 
1               5   


<210> 34
<211> 5
<212> PRT
<213> Emiliana huxleyi

<400> 34
Gln Thr Glu His His 
1               5   


<210> 35
<211> 789
<212> DNA
<213> Euglenoid deses

<400> 35
atggacgtcg cgactacgct ggctggcatc gcggcggacg tgctgccccg cgtggactac       60

gcgcggcttg ggcgcgacgc cgccgcctgc gaggttctat acctttcgct gttcttcatc      120

gccatgaagt tcatccttcg ccccctcggc gacaaggggc aggcccgcct caagtcgctc      180

ttcaccctct acaacctcgt gatgtccatc tactccctcg gatctttcgt tgtaatgggc      240

tacgccttgg cggatatcgg agtgctcggt ggtgattgcg ggaaagcatt ctcaaatccc      300

atgttccgcc tcaccgctca gttgttctac atcagcaagt acgttgagta catcgattcc      360

ttctacgtgc ttctcaccaa caagcccctg acctacctgc agttcttcca ccacctcgga      420

gcccccgtcg acctctggct cttcctgcag tacgaaaacg aggcgctgtg gatcttcgtc      480

ttcctcaacg gcttcatcca cttcatcatg tacgggtact actgggcccg gctggtgaag      540

ctcccgttcc ccgtgccgaa gtcgttcatc acctccatgc agatcatcca gttcaacctg      600

ggcttctacc tcgtgtggcg gtaccacaca atcccgtgct accgacagga cccaatgcga      660

atgttcgctt ggctcttcaa ctacttctac gtgggagtgg tcttactgct gtttttgaat      720

ttctacgtgc acacgtacgt gatcaagaaa gcgcggcggc tggcgaagga tgagaagaaa      780

gtggactag                                                              789


<210> 36
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic oligonucleotide

<400> 36
tatagaattc aaatggacgt cgcgactacg ctg                                    33


<210> 37
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic oligonucleotide 

<400> 37
tattctcgag ttctagtcca ctttcttctc atccttc                                37


<210> 38
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Asp or Glu

<220>
<221> MOD_RES
<222> (6)..(6)
<223> Ala or Gln

<220>
<221> MOD_RES
<222> (8)..(8)
<223> Glu or Met

<220>
<221> MOD_RES
<222> (9)..(9)
<223> Ser or Val

<220>
<221> MOD_RES
<222> (10)..(10)
<223> Tyr or Met

<400> 38
Arg Asp Ala Thr Xaa Xaa Phe Xaa Xaa Xaa His 
1               5                   10      


<210> 39
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide 


<220>
<221> MOD_RES
<222> (4)..(4)
<223> Ala or Ser

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Tyr or Ile

<220>
<221> MOD_RES
<222> (8)..(8)
<223> Leu or Ser

<400> 39
Gly Trp Leu Xaa His Asp Xaa Xaa His His 
1               5                   10  


<210> 40
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide 


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Lys or Arg

<220>
<221> MOD_RES
<222> (3)..(3)
<223> Ala or Leu

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Thr or Ala

<400> 40
Trp Xaa Xaa Arg His Asn Xaa His His 
1               5                   


<210> 41
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide 


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Ala or Gly

<220>
<221> MOD_RES
<222> (4)..(4)
<223> Ala or Gly

<220>
<221> MOD_RES 
<222> (5)..(5)
<223> Ile or Val

<400> 41
Phe Xaa Thr Xaa Xaa Val Val Phe Ala Thr His Tyr 
1               5                   10          


<210> 42
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide 


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Ile or Thr

<220>
<221> MOD_RES
<222> (9)..(9)
<223> Thr or Met

<400> 42
Gln Xaa Glu His His Leu Phe Pro Xaa Met Pro 
1               5                   10      


<210> 43
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 43
gctgtcaacg atacgctacg taacggcatg acagtgtttt tttttttttt tttttttttt       60


<210> 44
<211> 10
<212> PRT
<213> Emiliana huxleyi 

<400> 44
Pro Ala Gly Ser Ala Ser Pro Gly Arg Thr 
1               5                   10  


<210> 45
<211> 47
<212> PRT
<213> Emiliana huxleyi 

<400> 45
Pro Arg Arg Gly Arg Gln Arg Pro Arg Gly Pro His Gly Pro Pro Val 
1               5                   10                  15      


Glu Gly Ser Gly Val Ala Thr Gly Arg Glu Ile Ile Ile Glu Arg His 
            20                  25                  30          


Val Cys Trp Asp Ala Ile Gly Tyr Phe Gly Ile Trp Thr Gly Arg 
        35                  40                  45          


<210> 46
<211> 17
<212> PRT
<213> Emiliana huxleyi 

<400> 46
Arg Lys Lys Lys Lys Lys Lys Lys Lys His Met Pro Leu Arg Ser Glu 
1               5                   10                  15      


Gly 
    


