                         SEQUENCE LISTING

<110>  Howard Hughes Medical Institute
       Sternson, Scott M.
       Looger, Loren L.
       Lee, Peter    
 
<120>  Novel Chimeric Ligand-Gated Ion Channels and Methods of Use Thereof

<130>  HOWA-003/01WO

<150>  US 61/136,856
<151>  2008-10-09

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/mouse serotonin receptor 3 chimera

<400>  1

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Ile Ile Arg Arg Arg Pro Leu Phe Tyr Ala Val Ser Leu Leu Leu Pro 
225                 230                 235                 240 


Ser Ile Phe Leu Met Val Val Asp Ile Val Gly Phe Cys Leu Pro Pro 
                245                 250                 255     


Asp Ser Gly Glu Arg Val Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr 
            260                 265                 270         


Ser Val Phe Leu Ile Ile Val Ser Asp Thr Leu Pro Ala Thr Ile Gly 
        275                 280                 285             


Thr Pro Leu Ile Gly Val Tyr Phe Val Val Cys Met Ala Leu Leu Val 
    290                 295                 300                 


Ile Ser Leu Ala Glu Thr Ile Phe Ile Val Arg Leu Val His Lys Gln 
305                 310                 315                 320 


Asp Leu Gln Arg Pro Val Pro Asp Trp Leu Arg His Leu Val Leu Asp 
                325                 330                 335     


Arg Ile Ala Trp Ile Leu Cys Leu Gly Glu Gln Pro Met Ala His Arg 
            340                 345                 350         


Pro Pro Ala Thr Phe Gln Ala Asn Lys Thr Asp Asp Cys Ser Gly Ser 
        355                 360                 365             


Asp Leu Leu Pro Ala Met Gly Asn His Cys Ser His Val Gly Gly Pro 
    370                 375                 380                 


Gln Asp Leu Glu Lys Thr Pro Arg Gly Arg Gly Ser Pro Leu Pro Pro 
385                 390                 395                 400 


Pro Arg Glu Ala Ser Leu Ala Val Arg Gly Leu Leu Gln Glu Leu Ser 
                405                 410                 415     


Ser Ile Arg His Phe Leu Glu Lys Arg Asp Glu Met Arg Glu Val Ala 
            420                 425                 430         


Arg Asp Trp Leu Arg Val Gly Tyr Val Leu Asp Arg Leu Leu Phe Arg 
        435                 440                 445             


Ile Tyr Leu Leu Ala Val Leu Ala Tyr Ser Ile Thr Leu Val Thr Leu 
    450                 455                 460                 


Trp Ser Ile Trp His Tyr Ser 
465                 470     


<210>  2
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/mouse serotonin receptor 3 chimera W77F mutant

<400>  2

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Phe Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Ile Ile Arg Arg Arg Pro Leu Phe Tyr Ala Val Ser Leu Leu Leu Pro 
225                 230                 235                 240 


Ser Ile Phe Leu Met Val Val Asp Ile Val Gly Phe Cys Leu Pro Pro 
                245                 250                 255     


Asp Ser Gly Glu Arg Val Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr 
            260                 265                 270         


Ser Val Phe Leu Ile Ile Val Ser Asp Thr Leu Pro Ala Thr Ile Gly 
        275                 280                 285             


Thr Pro Leu Ile Gly Val Tyr Phe Val Val Cys Met Ala Leu Leu Val 
    290                 295                 300                 


Ile Ser Leu Ala Glu Thr Ile Phe Ile Val Arg Leu Val His Lys Gln 
305                 310                 315                 320 


Asp Leu Gln Arg Pro Val Pro Asp Trp Leu Arg His Leu Val Leu Asp 
                325                 330                 335     


Arg Ile Ala Trp Ile Leu Cys Leu Gly Glu Gln Pro Met Ala His Arg 
            340                 345                 350         


Pro Pro Ala Thr Phe Gln Ala Asn Lys Thr Asp Asp Cys Ser Gly Ser 
        355                 360                 365             


Asp Leu Leu Pro Ala Met Gly Asn His Cys Ser His Val Gly Gly Pro 
    370                 375                 380                 


Gln Asp Leu Glu Lys Thr Pro Arg Gly Arg Gly Ser Pro Leu Pro Pro 
385                 390                 395                 400 


Pro Arg Glu Ala Ser Leu Ala Val Arg Gly Leu Leu Gln Glu Leu Ser 
                405                 410                 415     


Ser Ile Arg His Phe Leu Glu Lys Arg Asp Glu Met Arg Glu Val Ala 
            420                 425                 430         


Arg Asp Trp Leu Arg Val Gly Tyr Val Leu Asp Arg Leu Leu Phe Arg 
        435                 440                 445             


Ile Tyr Leu Leu Ala Val Leu Ala Tyr Ser Ile Thr Leu Val Thr Leu 
    450                 455                 460                 


Trp Ser Ile Trp His Tyr Ser 
465                 470     


<210>  3
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/mouse serotonin receptor 3 chimera Q79G mutant

<400>  3

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gly Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Ile Ile Arg Arg Arg Pro Leu Phe Tyr Ala Val Ser Leu Leu Leu Pro 
225                 230                 235                 240 


Ser Ile Phe Leu Met Val Val Asp Ile Val Gly Phe Cys Leu Pro Pro 
                245                 250                 255     


Asp Ser Gly Glu Arg Val Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr 
            260                 265                 270         


Ser Val Phe Leu Ile Ile Val Ser Asp Thr Leu Pro Ala Thr Ile Gly 
        275                 280                 285             


Thr Pro Leu Ile Gly Val Tyr Phe Val Val Cys Met Ala Leu Leu Val 
    290                 295                 300                 


Ile Ser Leu Ala Glu Thr Ile Phe Ile Val Arg Leu Val His Lys Gln 
305                 310                 315                 320 


Asp Leu Gln Arg Pro Val Pro Asp Trp Leu Arg His Leu Val Leu Asp 
                325                 330                 335     


Arg Ile Ala Trp Ile Leu Cys Leu Gly Glu Gln Pro Met Ala His Arg 
            340                 345                 350         


Pro Pro Ala Thr Phe Gln Ala Asn Lys Thr Asp Asp Cys Ser Gly Ser 
        355                 360                 365             


Asp Leu Leu Pro Ala Met Gly Asn His Cys Ser His Val Gly Gly Pro 
    370                 375                 380                 


Gln Asp Leu Glu Lys Thr Pro Arg Gly Arg Gly Ser Pro Leu Pro Pro 
385                 390                 395                 400 


Pro Arg Glu Ala Ser Leu Ala Val Arg Gly Leu Leu Gln Glu Leu Ser 
                405                 410                 415     


Ser Ile Arg His Phe Leu Glu Lys Arg Asp Glu Met Arg Glu Val Ala 
            420                 425                 430         


Arg Asp Trp Leu Arg Val Gly Tyr Val Leu Asp Arg Leu Leu Phe Arg 
        435                 440                 445             


Ile Tyr Leu Leu Ala Val Leu Ala Tyr Ser Ile Thr Leu Val Thr Leu 
    450                 455                 460                 


Trp Ser Ile Trp His Tyr Ser 
465                 470     


<210>  4
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/mouse serotonin receptor 3 chimera L141F mutant

<400>  4

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Phe Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Ile Ile Arg Arg Arg Pro Leu Phe Tyr Ala Val Ser Leu Leu Leu Pro 
225                 230                 235                 240 


Ser Ile Phe Leu Met Val Val Asp Ile Val Gly Phe Cys Leu Pro Pro 
                245                 250                 255     


Asp Ser Gly Glu Arg Val Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr 
            260                 265                 270         


Ser Val Phe Leu Ile Ile Val Ser Asp Thr Leu Pro Ala Thr Ile Gly 
        275                 280                 285             


Thr Pro Leu Ile Gly Val Tyr Phe Val Val Cys Met Ala Leu Leu Val 
    290                 295                 300                 


Ile Ser Leu Ala Glu Thr Ile Phe Ile Val Arg Leu Val His Lys Gln 
305                 310                 315                 320 


Asp Leu Gln Arg Pro Val Pro Asp Trp Leu Arg His Leu Val Leu Asp 
                325                 330                 335     


Arg Ile Ala Trp Ile Leu Cys Leu Gly Glu Gln Pro Met Ala His Arg 
            340                 345                 350         


Pro Pro Ala Thr Phe Gln Ala Asn Lys Thr Asp Asp Cys Ser Gly Ser 
        355                 360                 365             


Asp Leu Leu Pro Ala Met Gly Asn His Cys Ser His Val Gly Gly Pro 
    370                 375                 380                 


Gln Asp Leu Glu Lys Thr Pro Arg Gly Arg Gly Ser Pro Leu Pro Pro 
385                 390                 395                 400 


Pro Arg Glu Ala Ser Leu Ala Val Arg Gly Leu Leu Gln Glu Leu Ser 
                405                 410                 415     


Ser Ile Arg His Phe Leu Glu Lys Arg Asp Glu Met Arg Glu Val Ala 
            420                 425                 430         


Arg Asp Trp Leu Arg Val Gly Tyr Val Leu Asp Arg Leu Leu Phe Arg 
        435                 440                 445             


Ile Tyr Leu Leu Ala Val Leu Ala Tyr Ser Ile Thr Leu Val Thr Leu 
    450                 455                 460                 


Trp Ser Ile Trp His Tyr Ser 
465                 470     


<210>  5
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/mouse serotonin receptor 3 chimera L141F/L276G 
       double mutant

<400>  5

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Phe Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Ile Ile Arg Arg Arg Pro Leu Phe Tyr Ala Val Ser Leu Leu Leu Pro 
225                 230                 235                 240 


Ser Ile Phe Leu Met Val Val Asp Ile Val Gly Phe Cys Leu Pro Pro 
                245                 250                 255     


Asp Ser Gly Glu Arg Val Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr 
            260                 265                 270         


Ser Val Phe Gly Ile Ile Val Ser Asp Thr Leu Pro Ala Thr Ile Gly 
        275                 280                 285             


Thr Pro Leu Ile Gly Val Tyr Phe Val Val Cys Met Ala Leu Leu Val 
    290                 295                 300                 


Ile Ser Leu Ala Glu Thr Ile Phe Ile Val Arg Leu Val His Lys Gln 
305                 310                 315                 320 


Asp Leu Gln Arg Pro Val Pro Asp Trp Leu Arg His Leu Val Leu Asp 
                325                 330                 335     


Arg Ile Ala Trp Ile Leu Cys Leu Gly Glu Gln Pro Met Ala His Arg 
            340                 345                 350         


Pro Pro Ala Thr Phe Gln Ala Asn Lys Thr Asp Asp Cys Ser Gly Ser 
        355                 360                 365             


Asp Leu Leu Pro Ala Met Gly Asn His Cys Ser His Val Gly Gly Pro 
    370                 375                 380                 


Gln Asp Leu Glu Lys Thr Pro Arg Gly Arg Gly Ser Pro Leu Pro Pro 
385                 390                 395                 400 


Pro Arg Glu Ala Ser Leu Ala Val Arg Gly Leu Leu Gln Glu Leu Ser 
                405                 410                 415     


Ser Ile Arg His Phe Leu Glu Lys Arg Asp Glu Met Arg Glu Val Ala 
            420                 425                 430         


Arg Asp Trp Leu Arg Val Gly Tyr Val Leu Asp Arg Leu Leu Phe Arg 
        435                 440                 445             


Ile Tyr Leu Leu Ala Val Leu Ala Tyr Ser Ile Thr Leu Val Thr Leu 
    450                 455                 460                 


Trp Ser Ile Trp His Tyr Ser 
465                 470     


<210>  6
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/human glycine receptor 1 chimera

<400>  6

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Met Gly Tyr Tyr Leu Ile Gln Met Tyr Ile Pro 
225                 230                 235                 240 


Ser Leu Leu Ile Val Ile Leu Ser Trp Ile Ser Phe Trp Ile Asn Met 
                245                 250                 255     


Asp Ala Ala Pro Ala Arg Val Gly Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Thr Thr Gln Ser Ser Gly Ser Arg Ala Ser Leu Pro Lys Val Ser 
        275                 280                 285             


Tyr Val Lys Ala Ile Asp Ile Trp Met Ala Val Cys Leu Leu Phe Val 
    290                 295                 300                 


Phe Ser Ala Leu Leu Glu Tyr Ala Ala Val Asn Phe Val Ser Arg Gln 
305                 310                 315                 320 


His Lys Glu Leu Leu Arg Phe Arg Arg Lys Arg Arg His His Lys Glu 
                325                 330                 335     


Asp Glu Ala Gly Glu Gly Arg Phe Asn Phe Ser Ala Tyr Gly Met Gly 
            340                 345                 350         


Pro Ala Cys Leu Gln Ala Lys Asp Gly Ile Ser Val Lys Gly Ala Asn 
        355                 360                 365             


Asn Ser Asn Thr Thr Asn Pro Pro Pro Ala Pro Ser Lys Ser Pro Glu 
    370                 375                 380                 


Glu Met Arg Lys Leu Phe Ile Gln Arg Ala Lys Lys Ile Asp Lys Ile 
385                 390                 395                 400 


Ser Arg Ile Gly Phe Pro Met Ala Phe Leu Ile Phe Asn Met Phe Tyr 
                405                 410                 415     


Trp Ile Ile Tyr Lys Ile Val Arg Arg Glu Asp Val His Asn Gln 
            420                 425                 430     


<210>  7
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/human glycine receptor 1 chimera W77F mutant

<400>  7

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Phe Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Met Gly Tyr Tyr Leu Ile Gln Met Tyr Ile Pro 
225                 230                 235                 240 


Ser Leu Leu Ile Val Ile Leu Ser Trp Ile Ser Phe Trp Ile Asn Met 
                245                 250                 255     


Asp Ala Ala Pro Ala Arg Val Gly Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Thr Thr Gln Ser Ser Gly Ser Arg Ala Ser Leu Pro Lys Val Ser 
        275                 280                 285             


Tyr Val Lys Ala Ile Asp Ile Trp Met Ala Val Cys Leu Leu Phe Val 
    290                 295                 300                 


Phe Ser Ala Leu Leu Glu Tyr Ala Ala Val Asn Phe Val Ser Arg Gln 
305                 310                 315                 320 


His Lys Glu Leu Leu Arg Phe Arg Arg Lys Arg Arg His His Lys Glu 
                325                 330                 335     


Asp Glu Ala Gly Glu Gly Arg Phe Asn Phe Ser Ala Tyr Gly Met Gly 
            340                 345                 350         


Pro Ala Cys Leu Gln Ala Lys Asp Gly Ile Ser Val Lys Gly Ala Asn 
        355                 360                 365             


Asn Ser Asn Thr Thr Asn Pro Pro Pro Ala Pro Ser Lys Ser Pro Glu 
    370                 375                 380                 


Glu Met Arg Lys Leu Phe Ile Gln Arg Ala Lys Lys Ile Asp Lys Ile 
385                 390                 395                 400 


Ser Arg Ile Gly Phe Pro Met Ala Phe Leu Ile Phe Asn Met Phe Tyr 
                405                 410                 415     


Trp Ile Ile Tyr Lys Ile Val Arg Arg Glu Asp Val His Asn Gln 
            420                 425                 430     


<210>  8
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/human glycine receptor 1 chimera Q79G mutant

<400>  8

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gly Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Met Gly Tyr Tyr Leu Ile Gln Met Tyr Ile Pro 
225                 230                 235                 240 


Ser Leu Leu Ile Val Ile Leu Ser Trp Ile Ser Phe Trp Ile Asn Met 
                245                 250                 255     


Asp Ala Ala Pro Ala Arg Val Gly Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Thr Thr Gln Ser Ser Gly Ser Arg Ala Ser Leu Pro Lys Val Ser 
        275                 280                 285             


Tyr Val Lys Ala Ile Asp Ile Trp Met Ala Val Cys Leu Leu Phe Val 
    290                 295                 300                 


Phe Ser Ala Leu Leu Glu Tyr Ala Ala Val Asn Phe Val Ser Arg Gln 
305                 310                 315                 320 


His Lys Glu Leu Leu Arg Phe Arg Arg Lys Arg Arg His His Lys Glu 
                325                 330                 335     


Asp Glu Ala Gly Glu Gly Arg Phe Asn Phe Ser Ala Tyr Gly Met Gly 
            340                 345                 350         


Pro Ala Cys Leu Gln Ala Lys Asp Gly Ile Ser Val Lys Gly Ala Asn 
        355                 360                 365             


Asn Ser Asn Thr Thr Asn Pro Pro Pro Ala Pro Ser Lys Ser Pro Glu 
    370                 375                 380                 


Glu Met Arg Lys Leu Phe Ile Gln Arg Ala Lys Lys Ile Asp Lys Ile 
385                 390                 395                 400 


Ser Arg Ile Gly Phe Pro Met Ala Phe Leu Ile Phe Asn Met Phe Tyr 
                405                 410                 415     


Trp Ile Ile Tyr Lys Ile Val Arg Arg Glu Asp Val His Asn Gln 
            420                 425                 430     


<210>  9
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human nAChR LBD/human glycine receptor 1 chimera L141F mutant

<400>  9

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Phe Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Met Gly Tyr Tyr Leu Ile Gln Met Tyr Ile Pro 
225                 230                 235                 240 


Ser Leu Leu Ile Val Ile Leu Ser Trp Ile Ser Phe Trp Ile Asn Met 
                245                 250                 255     


Asp Ala Ala Pro Ala Arg Val Gly Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Thr Thr Gln Ser Ser Gly Ser Arg Ala Ser Leu Pro Lys Val Ser 
        275                 280                 285             


Tyr Val Lys Ala Ile Asp Ile Trp Met Ala Val Cys Leu Leu Phe Val 
    290                 295                 300                 


Phe Ser Ala Leu Leu Glu Tyr Ala Ala Val Asn Phe Val Ser Arg Gln 
305                 310                 315                 320 


His Lys Glu Leu Leu Arg Phe Arg Arg Lys Arg Arg His His Lys Glu 
                325                 330                 335     


Asp Glu Ala Gly Glu Gly Arg Phe Asn Phe Ser Ala Tyr Gly Met Gly 
            340                 345                 350         


Pro Ala Cys Leu Gln Ala Lys Asp Gly Ile Ser Val Lys Gly Ala Asn 
        355                 360                 365             


Asn Ser Asn Thr Thr Asn Pro Pro Pro Ala Pro Ser Lys Ser Pro Glu 
    370                 375                 380                 


Glu Met Arg Lys Leu Phe Ile Gln Arg Ala Lys Lys Ile Asp Lys Ile 
385                 390                 395                 400 


Ser Arg Ile Gly Phe Pro Met Ala Phe Leu Ile Phe Asn Met Phe Tyr 
                405                 410                 415     


Trp Ile Ile Tyr Lys Ile Val Arg Arg Glu Asp Val His Asn Gln 
            420                 425                 430     


<210>  10
<211>  428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the wild-type chimeric receptor between 
       the alpha 7-nicotinic acetylcholine receptor and the GABA C ion 
       channel domain

<400>  10

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Thr Leu Tyr Tyr Leu Leu Gln Thr Tyr Phe Pro 
225                 230                 235                 240 


Ala Thr Leu Met Val Met Leu Ser Trp Val Ser Phe Trp Ile Asp Arg 
                245                 250                 255     


Arg Ala Val Pro Ala Arg Val Pro Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Ser Thr Ile Ile Thr Gly Val Asn Ala Ser Met Pro Arg Val Ser 
        275                 280                 285             


Tyr Ile Lys Ala Val Asp Ile Tyr Leu Trp Val Ser Phe Val Phe Val 
    290                 295                 300                 


Phe Leu Ser Val Leu Glu Tyr Ala Ala Val Asn Tyr Leu Thr Thr Val 
305                 310                 315                 320 


Gln Glu Arg Lys Glu Gln Lys Leu Arg Glu Lys Leu Pro Cys Thr Ser 
                325                 330                 335     


Gly Leu Pro Pro Pro Arg Thr Ala Met Leu Asp Gly Asn Tyr Ser Asp 
            340                 345                 350         


Gly Glu Val Asn Asp Leu Asp Asn Tyr Met Pro Glu Asn Gly Glu Lys 
        355                 360                 365             


Pro Asp Arg Met Met Val Gln Leu Thr Leu Ala Ser Glu Arg Ser Ser 
    370                 375                 380                 


Pro Gln Arg Lys Ser Gln Arg Ser Ser Tyr Val Ser Met Arg Ile Asp 
385                 390                 395                 400 


Thr His Ala Ile Asp Lys Tyr Ser Arg Ile Ile Phe Pro Ala Ala Tyr 
                405                 410                 415     


Ile Leu Phe Asn Leu Ile Tyr Trp Ser Ile Phe Ser 
            420                 425             


<210>  11
<211>  428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  W77F mutant amino acid sequence of the wild-type chimeric 
       receptor between the alpha 7-nicotinic acetylcholine receptor and
       the GABA C ion channel domain

<400>  11

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Phe Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Thr Leu Tyr Tyr Leu Leu Gln Thr Tyr Phe Pro 
225                 230                 235                 240 


Ala Thr Leu Met Val Met Leu Ser Trp Val Ser Phe Trp Ile Asp Arg 
                245                 250                 255     


Arg Ala Val Pro Ala Arg Val Pro Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Ser Thr Ile Ile Thr Gly Val Asn Ala Ser Met Pro Arg Val Ser 
        275                 280                 285             


Tyr Ile Lys Ala Val Asp Ile Tyr Leu Trp Val Ser Phe Val Phe Val 
    290                 295                 300                 


Phe Leu Ser Val Leu Glu Tyr Ala Ala Val Asn Tyr Leu Thr Thr Val 
305                 310                 315                 320 


Gln Glu Arg Lys Glu Gln Lys Leu Arg Glu Lys Leu Pro Cys Thr Ser 
                325                 330                 335     


Gly Leu Pro Pro Pro Arg Thr Ala Met Leu Asp Gly Asn Tyr Ser Asp 
            340                 345                 350         


Gly Glu Val Asn Asp Leu Asp Asn Tyr Met Pro Glu Asn Gly Glu Lys 
        355                 360                 365             


Pro Asp Arg Met Met Val Gln Leu Thr Leu Ala Ser Glu Arg Ser Ser 
    370                 375                 380                 


Pro Gln Arg Lys Ser Gln Arg Ser Ser Tyr Val Ser Met Arg Ile Asp 
385                 390                 395                 400 


Thr His Ala Ile Asp Lys Tyr Ser Arg Ile Ile Phe Pro Ala Ala Tyr 
                405                 410                 415     


Ile Leu Phe Asn Leu Ile Tyr Trp Ser Ile Phe Ser 
            420                 425             


<210>  12
<211>  428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Q79G mutant amino acid sequence of the wild-type chimeric 
       receptor between the alpha 7-nicotinic acetylcholine receptor and
       the GABA C ion channel domain

<400>  12

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gly Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Thr Leu Tyr Tyr Leu Leu Gln Thr Tyr Phe Pro 
225                 230                 235                 240 


Ala Thr Leu Met Val Met Leu Ser Trp Val Ser Phe Trp Ile Asp Arg 
                245                 250                 255     


Arg Ala Val Pro Ala Arg Val Pro Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Ser Thr Ile Ile Thr Gly Val Asn Ala Ser Met Pro Arg Val Ser 
        275                 280                 285             


Tyr Ile Lys Ala Val Asp Ile Tyr Leu Trp Val Ser Phe Val Phe Val 
    290                 295                 300                 


Phe Leu Ser Val Leu Glu Tyr Ala Ala Val Asn Tyr Leu Thr Thr Val 
305                 310                 315                 320 


Gln Glu Arg Lys Glu Gln Lys Leu Arg Glu Lys Leu Pro Cys Thr Ser 
                325                 330                 335     


Gly Leu Pro Pro Pro Arg Thr Ala Met Leu Asp Gly Asn Tyr Ser Asp 
            340                 345                 350         


Gly Glu Val Asn Asp Leu Asp Asn Tyr Met Pro Glu Asn Gly Glu Lys 
        355                 360                 365             


Pro Asp Arg Met Met Val Gln Leu Thr Leu Ala Ser Glu Arg Ser Ser 
    370                 375                 380                 


Pro Gln Arg Lys Ser Gln Arg Ser Ser Tyr Val Ser Met Arg Ile Asp 
385                 390                 395                 400 


Thr His Ala Ile Asp Lys Tyr Ser Arg Ile Ile Phe Pro Ala Ala Tyr 
                405                 410                 415     


Ile Leu Phe Asn Leu Ile Tyr Trp Ser Ile Phe Ser 
            420                 425             


<210>  13
<211>  428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L141F amino acid sequence of the wild-type chimeric receptor 
       between the alpha 7-nicotinic acetylcholine receptor and the GABA
       C ion channel domain

<400>  13

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Phe Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Thr Leu Tyr Tyr Leu Leu Gln Thr Tyr Phe Pro 
225                 230                 235                 240 


Ala Thr Leu Met Val Met Leu Ser Trp Val Ser Phe Trp Ile Asp Arg 
                245                 250                 255     


Arg Ala Val Pro Ala Arg Val Pro Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Ser Thr Ile Ile Thr Gly Val Asn Ala Ser Met Pro Arg Val Ser 
        275                 280                 285             


Tyr Ile Lys Ala Val Asp Ile Tyr Leu Trp Val Ser Phe Val Phe Val 
    290                 295                 300                 


Phe Leu Ser Val Leu Glu Tyr Ala Ala Val Asn Tyr Leu Thr Thr Val 
305                 310                 315                 320 


Gln Glu Arg Lys Glu Gln Lys Leu Arg Glu Lys Leu Pro Cys Thr Ser 
                325                 330                 335     


Gly Leu Pro Pro Pro Arg Thr Ala Met Leu Asp Gly Asn Tyr Ser Asp 
            340                 345                 350         


Gly Glu Val Asn Asp Leu Asp Asn Tyr Met Pro Glu Asn Gly Glu Lys 
        355                 360                 365             


Pro Asp Arg Met Met Val Gln Leu Thr Leu Ala Ser Glu Arg Ser Ser 
    370                 375                 380                 


Pro Gln Arg Lys Ser Gln Arg Ser Ser Tyr Val Ser Met Arg Ile Asp 
385                 390                 395                 400 


Thr His Ala Ile Asp Lys Tyr Ser Arg Ile Ile Phe Pro Ala Ala Tyr 
                405                 410                 415     


Ile Leu Phe Asn Leu Ile Tyr Trp Ser Ile Phe Ser 
            420                 425             


