                         SEQUENCE LISTING

<110>  Tocagen Inc.
       Gruber, Harry E.
       Jolly, Douglas
       Perez, Omar
 
<120>  GENE THERAPY VECTORS AND CYTOSINE DEAMINASES

<130>  00014-007WO1

<140>  Not yet known
<141>  2009-09-26

<150>  US 61/100,666
<151>  2008-09-26

<150>  US 61/120,618
<151>  2008-12-08

<150>  US 61/186,823
<151>  2009-06-13

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  477
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(477)

<400>  1
atg gtg aca ggg gga atg gca agc aag tgg gat cag aag ggt atg gac         48
Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp           
1               5                   10                  15                

att gcc tat gag gag gcg gcc tta ggt tac aaa gag ggt ggt gtt cct         96
Ile Ala Tyr Glu Glu Ala Ala Leu Gly Tyr Lys Glu Gly Gly Val Pro           
            20                  25                  30                    

att ggc gga tgt ctt atc aat aac aaa gac gga agt gtt ctc ggt cgt        144
Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg           
        35                  40                  45                        

ggt cac aac atg aga ttt caa aag gga tcc gcc aca cta cat ggt gag        192
Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu           
    50                  55                  60                            

atc tcc act ttg gaa aac tgt ggg aga tta gag ggc aaa gtg tac aaa        240
Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys           
65                  70                  75                  80            

gat acc act ttg tat acg acg ctg tct cca tgc gac atg tgt aca ggt        288
Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly           
                85                  90                  95                

gcc atc atc atg tat ggt att cca cgc tgt gtt gtc ggt gag aac gtt        336
Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Val Gly Glu Asn Val           
            100                 105                 110                   

aat ttc aaa agt aag ggc gag aaa tat tta caa act aga ggt cac gag        384
Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu           
        115                 120                 125                       

gtt gtt gtt gtt gac gat gag agg tgt aaa aag atc atg aaa caa ttt        432
Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Ile Met Lys Gln Phe           
    130                 135                 140                           

atc gat gaa aga cct cag gat tgg ttt gaa gat att ggt gag tag            477
Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu                   
145                 150                 155                               


<210>  2
<211>  158
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp 
1               5                   10                  15      


Ile Ala Tyr Glu Glu Ala Ala Leu Gly Tyr Lys Glu Gly Gly Val Pro 
            20                  25                  30          


Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg 
        35                  40                  45              


Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu 
    50                  55                  60                  


Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys 
65                  70                  75                  80  


Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly 
                85                  90                  95      


Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Val Gly Glu Asn Val 
            100                 105                 110         


Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu 
        115                 120                 125             


Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Ile Met Lys Gln Phe 
    130                 135                 140                 


Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu 
145                 150                 155             


<210>  3
<211>  477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered cytosine deaminase


<220>
<221>  CDS
<222>  (1)..(477)

<400>  3
atg gtg aca ggg gga atg gca agc aag tgg gat cag aag ggt atg gac         48
Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp           
1               5                   10                  15                

att gcc tat gag gag gcg tta tta ggt tac aaa gag ggt ggt gtt cct         96
Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro           
            20                  25                  30                    

att ggc gga tgt ctt atc aat aac aaa gac gga agt gtt ctc ggt cgt        144
Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg           
        35                  40                  45                        

ggt cac aac atg aga ttt caa aag gga tcc gcc aca cta cat ggt gag        192
Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu           
    50                  55                  60                            

atc tcc act ttg gaa aac tgt ggg aga tta gag ggc aaa gtg tac aaa        240
Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys           
65                  70                  75                  80            

gat acc act ttg tat acg acg ctg tct cca tgc gac atg tgt aca ggt        288
Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly           
                85                  90                  95                

gcc atc atc atg tat ggt att cca cgc tgt gtc atc ggt gag aac gtt        336
Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val           
            100                 105                 110                   

aat ttc aaa agt aag ggc gag aaa tat tta caa act aga ggt cac gag        384
Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu           
        115                 120                 125                       

gtt gtt gtt gtt gac gat gag agg tgt aaa aag tta atg aaa caa ttt        432
Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe           
    130                 135                 140                           

atc gat gaa aga cct cag gat tgg ttt gaa gat att ggt gag tag            477
Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu                   
145                 150                 155                               


<210>  4
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp 
1               5                   10                  15      


Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro 
            20                  25                  30          


Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg 
        35                  40                  45              


Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu 
    50                  55                  60                  


Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys 
65                  70                  75                  80  


Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly 
                85                  90                  95      


Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val 
            100                 105                 110         


Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu 
        115                 120                 125             


Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe 
    130                 135                 140                 


Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu 
145                 150                 155             


<210>  5
<211>  480
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human codon optimized cytosine deaminase


<220>
<221>  CDS
<222>  (1)..(480)

<400>  5
atg gtg acc ggc ggc atg gcc tcc aag tgg gat caa aag ggc atg gat         48
Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp           
1               5                   10                  15                

atc gct tac gag gag gcc gca ctg ggc tac aag gag ggc ggc gtg cct         96
Ile Ala Tyr Glu Glu Ala Ala Leu Gly Tyr Lys Glu Gly Gly Val Pro           
            20                  25                  30                    

atc ggc ggc tgt ctg atc aac aac aag gac ggc agt gtg ctg ggc agg        144
Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg           
        35                  40                  45                        

ggc cac aac atg agg ttc cag aag ggc tcc gcc acc ctg cac ggc gag        192
Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu           
    50                  55                  60                            

atc tcc acc ctg gag aac tgt ggc agg ctg gag ggc aag gtg tac aag        240
Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys           
65                  70                  75                  80            

gac acc acc ctg tac acc acc ctg tcc cct tgt gac atg tgt acc ggc        288
Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly           
                85                  90                  95                

gct atc atc atg tac ggc atc cct agg tgt gtg gtc ggc gag aac gtg        336
Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Val Gly Glu Asn Val           
            100                 105                 110                   

aac ttc aag tcc aag ggc gag aag tac ctg caa acc agg ggc cac gag        384
Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu           
        115                 120                 125                       

gtg gtg gtt gtt gac gat gag agg tgt aag aag atc atg aag cag ttc        432
Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Ile Met Lys Gln Phe           
    130                 135                 140                           

atc gac gag agg cct cag gac tgg ttc gag gat atc ggc gag tga taa        480
Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu                   
145                 150                 155                               


<210>  6
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp 
1               5                   10                  15      


Ile Ala Tyr Glu Glu Ala Ala Leu Gly Tyr Lys Glu Gly Gly Val Pro 
            20                  25                  30          


Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg 
        35                  40                  45              


Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu 
    50                  55                  60                  


Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys 
65                  70                  75                  80  


Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly 
                85                  90                  95      


Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Val Gly Glu Asn Val 
            100                 105                 110         


Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu 
        115                 120                 125             


Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Ile Met Lys Gln Phe 
    130                 135                 140                 


Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu 
145                 150                 155             


<210>  7
<211>  756
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(756)

<400>  7
atg aac ccg tta ttc ttt ttg gct tct cca ttc ttg tac ctt aca tat         48
Met Asn Pro Leu Phe Phe Leu Ala Ser Pro Phe Leu Tyr Leu Thr Tyr           
1               5                   10                  15                

ctt ata tat tat cca aac aaa ggg tct ttc gtt agc aaa cct aga aat         96
Leu Ile Tyr Tyr Pro Asn Lys Gly Ser Phe Val Ser Lys Pro Arg Asn           
            20                  25                  30                    

ctg caa aaa atg tct tcg gaa cca ttt aag aac gtc tac ttg cta cct        144
Leu Gln Lys Met Ser Ser Glu Pro Phe Lys Asn Val Tyr Leu Leu Pro           
        35                  40                  45                        

caa aca aac caa ttg ctg ggt ttg tac acc atc atc aga aat aag aat        192
Gln Thr Asn Gln Leu Leu Gly Leu Tyr Thr Ile Ile Arg Asn Lys Asn           
    50                  55                  60                            

aca act aga cct gat ttc att ttc tac tcc gat aga atc atc aga ttg        240
Thr Thr Arg Pro Asp Phe Ile Phe Tyr Ser Asp Arg Ile Ile Arg Leu           
65                  70                  75                  80            

ttg gtt gaa gaa ggt ttg aac cat cta cct gtg caa aag caa att gtg        288
Leu Val Glu Glu Gly Leu Asn His Leu Pro Val Gln Lys Gln Ile Val           
                85                  90                  95                

gaa act gac acc aac gaa aac ttc gaa ggt gtc tca ttc atg ggt aaa        336
Glu Thr Asp Thr Asn Glu Asn Phe Glu Gly Val Ser Phe Met Gly Lys           
            100                 105                 110                   

atc tgt ggt gtt tcc att gtc aga gct ggt gaa tcg atg gag caa gga        384
Ile Cys Gly Val Ser Ile Val Arg Ala Gly Glu Ser Met Glu Gln Gly           
        115                 120                 125                       

tta aga gac tgt tgt agg tct gtg cgt atc ggt aaa att tta att caa        432
Leu Arg Asp Cys Cys Arg Ser Val Arg Ile Gly Lys Ile Leu Ile Gln           
    130                 135                 140                           

agg gac gag gag act gct tta cca aag tta ttc tac gaa aaa tta cca        480
Arg Asp Glu Glu Thr Ala Leu Pro Lys Leu Phe Tyr Glu Lys Leu Pro           
145                 150                 155                 160           

gag gat ata tct gaa agg tat gtc ttc cta tta gac cca atg ctg gcc        528
Glu Asp Ile Ser Glu Arg Tyr Val Phe Leu Leu Asp Pro Met Leu Ala           
                165                 170                 175               

acc ggt ggt agt gct atc atg gct aca gaa gtc ttg att aag aga ggt        576
Thr Gly Gly Ser Ala Ile Met Ala Thr Glu Val Leu Ile Lys Arg Gly           
            180                 185                 190                   

gtt aag cca gag aga att tac ttc tta aac cta atc tgt agt aag gaa        624
Val Lys Pro Glu Arg Ile Tyr Phe Leu Asn Leu Ile Cys Ser Lys Glu           
        195                 200                 205                       

ggg att gaa aaa tac cat gcc gcc ttc cca gag gtc aga att gtt act        672
Gly Ile Glu Lys Tyr His Ala Ala Phe Pro Glu Val Arg Ile Val Thr           
    210                 215                 220                           

ggt gcc ctc gac aga ggt cta gat gaa aac aag tat cta gtt cca ggg        720
Gly Ala Leu Asp Arg Gly Leu Asp Glu Asn Lys Tyr Leu Val Pro Gly           
225                 230                 235                 240           

ttg ggt gac ttt ggt gac aga tac tac tgt gtt taa                        756
Leu Gly Asp Phe Gly Asp Arg Tyr Tyr Cys Val                               
                245                 250                                   


<210>  8
<211>  251
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  8

Met Asn Pro Leu Phe Phe Leu Ala Ser Pro Phe Leu Tyr Leu Thr Tyr 
1               5                   10                  15      


Leu Ile Tyr Tyr Pro Asn Lys Gly Ser Phe Val Ser Lys Pro Arg Asn 
            20                  25                  30          


Leu Gln Lys Met Ser Ser Glu Pro Phe Lys Asn Val Tyr Leu Leu Pro 
        35                  40                  45              


Gln Thr Asn Gln Leu Leu Gly Leu Tyr Thr Ile Ile Arg Asn Lys Asn 
    50                  55                  60                  


Thr Thr Arg Pro Asp Phe Ile Phe Tyr Ser Asp Arg Ile Ile Arg Leu 
65                  70                  75                  80  


Leu Val Glu Glu Gly Leu Asn His Leu Pro Val Gln Lys Gln Ile Val 
                85                  90                  95      


Glu Thr Asp Thr Asn Glu Asn Phe Glu Gly Val Ser Phe Met Gly Lys 
            100                 105                 110         


Ile Cys Gly Val Ser Ile Val Arg Ala Gly Glu Ser Met Glu Gln Gly 
        115                 120                 125             


Leu Arg Asp Cys Cys Arg Ser Val Arg Ile Gly Lys Ile Leu Ile Gln 
    130                 135                 140                 


Arg Asp Glu Glu Thr Ala Leu Pro Lys Leu Phe Tyr Glu Lys Leu Pro 
145                 150                 155                 160 


Glu Asp Ile Ser Glu Arg Tyr Val Phe Leu Leu Asp Pro Met Leu Ala 
                165                 170                 175     


Thr Gly Gly Ser Ala Ile Met Ala Thr Glu Val Leu Ile Lys Arg Gly 
            180                 185                 190         


Val Lys Pro Glu Arg Ile Tyr Phe Leu Asn Leu Ile Cys Ser Lys Glu 
        195                 200                 205             


Gly Ile Glu Lys Tyr His Ala Ala Phe Pro Glu Val Arg Ile Val Thr 
    210                 215                 220                 


Gly Ala Leu Asp Arg Gly Leu Asp Glu Asn Lys Tyr Leu Val Pro Gly 
225                 230                 235                 240 


Leu Gly Asp Phe Gly Asp Arg Tyr Tyr Cys Val 
                245                 250     


<210>  9
<211>  1443
<212>  DNA
<213>  homo sapiens


<220>
<221>  CDS
<222>  (1)..(1443)

<400>  9
atg gct gtt gct cgt gct gct ctt ggt cct ctt gtt act ggt ctt tat         48
Met Ala Val Ala Arg Ala Ala Leu Gly Pro Leu Val Thr Gly Leu Tyr           
1               5                   10                  15                

gat gtt caa gct ttt aaa ttt ggt gat ttt gtt ctt aaa tct ggt ctt         96
Asp Val Gln Ala Phe Lys Phe Gly Asp Phe Val Leu Lys Ser Gly Leu           
            20                  25                  30                    

tct tct cct att tat att gat ctt cgt ggt att gtt tct cgt cct cgt        144
Ser Ser Pro Ile Tyr Ile Asp Leu Arg Gly Ile Val Ser Arg Pro Arg           
        35                  40                  45                        

ctt ctt tct caa gtt gct gat att ctt ttt caa act gct caa aat gct        192
Leu Leu Ser Gln Val Ala Asp Ile Leu Phe Gln Thr Ala Gln Asn Ala           
    50                  55                  60                            

ggt att tct ttt gat act gtt tgt ggt gtt cct tat act gct ctt cct        240
Gly Ile Ser Phe Asp Thr Val Cys Gly Val Pro Tyr Thr Ala Leu Pro           
65                  70                  75                  80            

ctt gct act gtt att tgt tct act aat caa att cct atg ctt att cgt        288
Leu Ala Thr Val Ile Cys Ser Thr Asn Gln Ile Pro Met Leu Ile Arg           
                85                  90                  95                

cgt aaa gaa act aaa gat tat ggt act aaa cgt ctt gtt gaa ggt act        336
Arg Lys Glu Thr Lys Asp Tyr Gly Thr Lys Arg Leu Val Glu Gly Thr           
            100                 105                 110                   

att aat cct ggt gaa act tgt ctt att att gaa gat gtt gtt act tct        384
Ile Asn Pro Gly Glu Thr Cys Leu Ile Ile Glu Asp Val Val Thr Ser           
        115                 120                 125                       

ggt tct tct gtt ctt gaa act gtt gaa gtt ctt caa aaa gaa ggt ctt        432
Gly Ser Ser Val Leu Glu Thr Val Glu Val Leu Gln Lys Glu Gly Leu           
    130                 135                 140                           

aaa gtt act gat gct att gtt ctt ctt gat cgt gaa caa ggt ggt aaa        480
Lys Val Thr Asp Ala Ile Val Leu Leu Asp Arg Glu Gln Gly Gly Lys           
145                 150                 155                 160           

gat aaa ctt caa gct cat ggt att cgt ctt cat tct gtt tgt act ctt        528
Asp Lys Leu Gln Ala His Gly Ile Arg Leu His Ser Val Cys Thr Leu           
                165                 170                 175               

tct aaa atg ctt gaa att ctt gaa caa caa aaa aaa gtt gat gct gaa        576
Ser Lys Met Leu Glu Ile Leu Glu Gln Gln Lys Lys Val Asp Ala Glu           
            180                 185                 190                   

act gtt ggt cgt gtt aaa cgt ttt att caa gaa aat gtt ttt gtt gct        624
Thr Val Gly Arg Val Lys Arg Phe Ile Gln Glu Asn Val Phe Val Ala           
        195                 200                 205                       

gct aat cat aat ggt tct cct ctt tct att aaa gaa gct cct aaa gaa        672
Ala Asn His Asn Gly Ser Pro Leu Ser Ile Lys Glu Ala Pro Lys Glu           
    210                 215                 220                           

ctt tct ttt ggt gct cgt gct gaa ctt cct cgt att cat cct gtt gct        720
Leu Ser Phe Gly Ala Arg Ala Glu Leu Pro Arg Ile His Pro Val Ala           
225                 230                 235                 240           

tct aaa ctt ctt cgt ctt atg caa aaa aaa gaa act aat ctt tgt ctt        768
Ser Lys Leu Leu Arg Leu Met Gln Lys Lys Glu Thr Asn Leu Cys Leu           
                245                 250                 255               

tct gct gat gtt tct ctt gct cgt gaa ctt ctt caa ctt gct gat gct        816
Ser Ala Asp Val Ser Leu Ala Arg Glu Leu Leu Gln Leu Ala Asp Ala           
            260                 265                 270                   

ctt ggt cct tct att tgt atg ctt aaa act cat gtt gat att ctt aat        864
Leu Gly Pro Ser Ile Cys Met Leu Lys Thr His Val Asp Ile Leu Asn           
        275                 280                 285                       

gat ttt act ctt gat gtt atg aaa gaa ctt att act ctt gct aaa tgt        912
Asp Phe Thr Leu Asp Val Met Lys Glu Leu Ile Thr Leu Ala Lys Cys           
    290                 295                 300                           

cat gaa ttt ctt att ttt gaa gat cgt aaa ttt gct gat att ggt aat        960
His Glu Phe Leu Ile Phe Glu Asp Arg Lys Phe Ala Asp Ile Gly Asn           
305                 310                 315                 320           

act gtt aaa aaa caa tat gaa ggt ggt att ttt aaa att gct tct tgg       1008
Thr Val Lys Lys Gln Tyr Glu Gly Gly Ile Phe Lys Ile Ala Ser Trp           
                325                 330                 335               

gct gat ctt gtt aat gct cat gtt gtt cct ggt tct ggt gtt gtt aaa       1056
Ala Asp Leu Val Asn Ala His Val Val Pro Gly Ser Gly Val Val Lys           
            340                 345                 350                   

ggt ctt caa gaa gtt ggt ctt cct ctt cat cgt ggt tgt ctt ctt att       1104
Gly Leu Gln Glu Val Gly Leu Pro Leu His Arg Gly Cys Leu Leu Ile           
        355                 360                 365                       

gct gaa atg tct tct act ggt tct ctt gct act ggt gat tat act cgt       1152
Ala Glu Met Ser Ser Thr Gly Ser Leu Ala Thr Gly Asp Tyr Thr Arg           
    370                 375                 380                           

gct gct gtt cgt atg gct gaa gaa cat tct gaa ttt gtt gtt ggt ttt       1200
Ala Ala Val Arg Met Ala Glu Glu His Ser Glu Phe Val Val Gly Phe           
385                 390                 395                 400           

att tct ggt tct cgt gtt tct atg aaa cct gaa ttt ctt cat ctt act       1248
Ile Ser Gly Ser Arg Val Ser Met Lys Pro Glu Phe Leu His Leu Thr           
                405                 410                 415               

cct ggt gtt caa ctt gaa gct ggt ggt gat aat ctt ggt caa caa tat       1296
Pro Gly Val Gln Leu Glu Ala Gly Gly Asp Asn Leu Gly Gln Gln Tyr           
            420                 425                 430                   

aat tct cct caa gaa gtt att ggt aaa cgt ggt tct gat att att att       1344
Asn Ser Pro Gln Glu Val Ile Gly Lys Arg Gly Ser Asp Ile Ile Ile           
        435                 440                 445                       

gtt ggt cgt ggt att att tct gct gct gat cgt ctt gaa gct gct gaa       1392
Val Gly Arg Gly Ile Ile Ser Ala Ala Asp Arg Leu Glu Ala Ala Glu           
    450                 455                 460                           

atg tat cgt aaa gct gct tgg gaa gct tat ctt tct cgt ctt ggt gtt       1440
Met Tyr Arg Lys Ala Ala Trp Glu Ala Tyr Leu Ser Arg Leu Gly Val           
465                 470                 475                 480           

taa                                                                   1443


<210>  10
<211>  480
<212>  PRT
<213>  homo sapiens

<400>  10

Met Ala Val Ala Arg Ala Ala Leu Gly Pro Leu Val Thr Gly Leu Tyr 
1               5                   10                  15      


Asp Val Gln Ala Phe Lys Phe Gly Asp Phe Val Leu Lys Ser Gly Leu 
            20                  25                  30          


Ser Ser Pro Ile Tyr Ile Asp Leu Arg Gly Ile Val Ser Arg Pro Arg 
        35                  40                  45              


Leu Leu Ser Gln Val Ala Asp Ile Leu Phe Gln Thr Ala Gln Asn Ala 
    50                  55                  60                  


Gly Ile Ser Phe Asp Thr Val Cys Gly Val Pro Tyr Thr Ala Leu Pro 
65                  70                  75                  80  


Leu Ala Thr Val Ile Cys Ser Thr Asn Gln Ile Pro Met Leu Ile Arg 
                85                  90                  95      


Arg Lys Glu Thr Lys Asp Tyr Gly Thr Lys Arg Leu Val Glu Gly Thr 
            100                 105                 110         


Ile Asn Pro Gly Glu Thr Cys Leu Ile Ile Glu Asp Val Val Thr Ser 
        115                 120                 125             


Gly Ser Ser Val Leu Glu Thr Val Glu Val Leu Gln Lys Glu Gly Leu 
    130                 135                 140                 


Lys Val Thr Asp Ala Ile Val Leu Leu Asp Arg Glu Gln Gly Gly Lys 
145                 150                 155                 160 


Asp Lys Leu Gln Ala His Gly Ile Arg Leu His Ser Val Cys Thr Leu 
                165                 170                 175     


Ser Lys Met Leu Glu Ile Leu Glu Gln Gln Lys Lys Val Asp Ala Glu 
            180                 185                 190         


Thr Val Gly Arg Val Lys Arg Phe Ile Gln Glu Asn Val Phe Val Ala 
        195                 200                 205             


Ala Asn His Asn Gly Ser Pro Leu Ser Ile Lys Glu Ala Pro Lys Glu 
    210                 215                 220                 


Leu Ser Phe Gly Ala Arg Ala Glu Leu Pro Arg Ile His Pro Val Ala 
225                 230                 235                 240 


Ser Lys Leu Leu Arg Leu Met Gln Lys Lys Glu Thr Asn Leu Cys Leu 
                245                 250                 255     


Ser Ala Asp Val Ser Leu Ala Arg Glu Leu Leu Gln Leu Ala Asp Ala 
            260                 265                 270         


Leu Gly Pro Ser Ile Cys Met Leu Lys Thr His Val Asp Ile Leu Asn 
        275                 280                 285             


Asp Phe Thr Leu Asp Val Met Lys Glu Leu Ile Thr Leu Ala Lys Cys 
    290                 295                 300                 


His Glu Phe Leu Ile Phe Glu Asp Arg Lys Phe Ala Asp Ile Gly Asn 
305                 310                 315                 320 


Thr Val Lys Lys Gln Tyr Glu Gly Gly Ile Phe Lys Ile Ala Ser Trp 
                325                 330                 335     


Ala Asp Leu Val Asn Ala His Val Val Pro Gly Ser Gly Val Val Lys 
            340                 345                 350         


Gly Leu Gln Glu Val Gly Leu Pro Leu His Arg Gly Cys Leu Leu Ile 
        355                 360                 365             


Ala Glu Met Ser Ser Thr Gly Ser Leu Ala Thr Gly Asp Tyr Thr Arg 
    370                 375                 380                 


Ala Ala Val Arg Met Ala Glu Glu His Ser Glu Phe Val Val Gly Phe 
385                 390                 395                 400 


Ile Ser Gly Ser Arg Val Ser Met Lys Pro Glu Phe Leu His Leu Thr 
                405                 410                 415     


Pro Gly Val Gln Leu Glu Ala Gly Gly Asp Asn Leu Gly Gln Gln Tyr 
            420                 425                 430         


Asn Ser Pro Gln Glu Val Ile Gly Lys Arg Gly Ser Asp Ile Ile Ile 
        435                 440                 445             


Val Gly Arg Gly Ile Ile Ser Ala Ala Asp Arg Leu Glu Ala Ala Glu 
    450                 455                 460                 


Met Tyr Arg Lys Ala Ala Trp Glu Ala Tyr Leu Ser Arg Leu Gly Val 
465                 470                 475                 480 


<210>  11
<211>  1227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion construct CDopt-UPRT


<220>
<221>  CDS
<222>  (1)..(1227)

<400>  11
atg gtg acc ggc ggc atg gcc tcc aag tgg gat caa aag ggc atg gat         48
Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp           
1               5                   10                  15                

atc gct tac gag gag gcc ctg ctg ggc tac aag gag ggc ggc gtg cct         96
Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro           
            20                  25                  30                    

atc ggc ggc tgt ctg atc aac aac aag gac ggc agt gtg ctg ggc agg        144
Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg           
        35                  40                  45                        

ggc cac aac atg agg ttc cag aag ggc tcc gcc acc ctg cac ggc gag        192
Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu           
    50                  55                  60                            

atc tcc acc ctg gag aac tgt ggc agg ctg gag ggc aag gtg tac aag        240
Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys           
65                  70                  75                  80            

gac acc acc ctg tac acc acc ctg tcc cct tgt gac atg tgt acc ggc        288
Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly           
                85                  90                  95                

gct atc atc atg tac ggc atc cct agg tgt gtg atc ggc gag aac gtg        336
Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val           
            100                 105                 110                   

aac ttc aag tcc aag ggc gag aag tac ctg caa acc agg ggc cac gag        384
Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu           
        115                 120                 125                       

gtg gtg gtt gtt gac gat gag agg tgt aag aag ctg atg aag cag ttc        432
Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe           
    130                 135                 140                           

atc gac gag agg cct cag gac tgg ttc gag gat atc ggc gag aac ccg        480
Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu Asn Pro           
145                 150                 155                 160           

tta ttc ttt ttg gct tct cca ttc ttg tac ctt aca tat ctt ata tat        528
Leu Phe Phe Leu Ala Ser Pro Phe Leu Tyr Leu Thr Tyr Leu Ile Tyr           
                165                 170                 175               

tat cca aac aaa ggg tct ttc gtt agc aaa cct aga aat ctg caa aaa        576
Tyr Pro Asn Lys Gly Ser Phe Val Ser Lys Pro Arg Asn Leu Gln Lys           
            180                 185                 190                   

atg tct tcg gaa cca ttt aag aac gtc tac ttg cta cct caa aca aac        624
Met Ser Ser Glu Pro Phe Lys Asn Val Tyr Leu Leu Pro Gln Thr Asn           
        195                 200                 205                       

caa ttg ctg ggt ttg tac acc atc atc aga aat aag aat aca act aga        672
Gln Leu Leu Gly Leu Tyr Thr Ile Ile Arg Asn Lys Asn Thr Thr Arg           
    210                 215                 220                           

cct gat ttc att ttc tac tcc gat aga atc atc aga ttg ttg gtt gaa        720
Pro Asp Phe Ile Phe Tyr Ser Asp Arg Ile Ile Arg Leu Leu Val Glu           
225                 230                 235                 240           

gaa ggt ttg aac cat cta cct gtg caa aag caa att gtg gaa act gac        768
Glu Gly Leu Asn His Leu Pro Val Gln Lys Gln Ile Val Glu Thr Asp           
                245                 250                 255               

acc aac gaa aac ttc gaa ggt gtc tca ttc atg ggt aaa atc tgt ggt        816
Thr Asn Glu Asn Phe Glu Gly Val Ser Phe Met Gly Lys Ile Cys Gly           
            260                 265                 270                   

gtt tcc att gtc aga gct ggt gaa tcg atg gag caa gga tta aga gac        864
Val Ser Ile Val Arg Ala Gly Glu Ser Met Glu Gln Gly Leu Arg Asp           
        275                 280                 285                       

tgt tgt agg tct gtg cgt atc ggt aaa att tta att caa agg gac gag        912
Cys Cys Arg Ser Val Arg Ile Gly Lys Ile Leu Ile Gln Arg Asp Glu           
    290                 295                 300                           

gag act gct tta cca aag tta ttc tac gaa aaa tta cca gag gat ata        960
Glu Thr Ala Leu Pro Lys Leu Phe Tyr Glu Lys Leu Pro Glu Asp Ile           
305                 310                 315                 320           

tct gaa agg tat gtc ttc cta tta gac cca atg ctg gcc acc ggt ggt       1008
Ser Glu Arg Tyr Val Phe Leu Leu Asp Pro Met Leu Ala Thr Gly Gly           
                325                 330                 335               

agt gct atc atg gct aca gaa gtc ttg att aag aga ggt gtt aag cca       1056
Ser Ala Ile Met Ala Thr Glu Val Leu Ile Lys Arg Gly Val Lys Pro           
            340                 345                 350                   

gag aga att tac ttc tta aac cta atc tgt agt aag gaa ggg att gaa       1104
Glu Arg Ile Tyr Phe Leu Asn Leu Ile Cys Ser Lys Glu Gly Ile Glu           
        355                 360                 365                       

aaa tac cat gcc gcc ttc cca gag gtc aga att gtt act ggt gcc ctc       1152
Lys Tyr His Ala Ala Phe Pro Glu Val Arg Ile Val Thr Gly Ala Leu           
    370                 375                 380                           

gac aga ggt cta gat gaa aac aag tat cta gtt cca ggg ttg ggt gac       1200
Asp Arg Gly Leu Asp Glu Asn Lys Tyr Leu Val Pro Gly Leu Gly Asp           
385                 390                 395                 400           

ttt ggt gac aga tac tac tgt gtt taa                                   1227
Phe Gly Asp Arg Tyr Tyr Cys Val                                           
                405                                                       


<210>  12
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp 
1               5                   10                  15      


Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro 
            20                  25                  30          


Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg 
        35                  40                  45              


Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu 
    50                  55                  60                  


Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys 
65                  70                  75                  80  


Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly 
                85                  90                  95      


Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val 
            100                 105                 110         


Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu 
        115                 120                 125             


Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe 
    130                 135                 140                 


Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu Asn Pro 
145                 150                 155                 160 


Leu Phe Phe Leu Ala Ser Pro Phe Leu Tyr Leu Thr Tyr Leu Ile Tyr 
                165                 170                 175     


Tyr Pro Asn Lys Gly Ser Phe Val Ser Lys Pro Arg Asn Leu Gln Lys 
            180                 185                 190         


Met Ser Ser Glu Pro Phe Lys Asn Val Tyr Leu Leu Pro Gln Thr Asn 
        195                 200                 205             


Gln Leu Leu Gly Leu Tyr Thr Ile Ile Arg Asn Lys Asn Thr Thr Arg 
    210                 215                 220                 


Pro Asp Phe Ile Phe Tyr Ser Asp Arg Ile Ile Arg Leu Leu Val Glu 
225                 230                 235                 240 


Glu Gly Leu Asn His Leu Pro Val Gln Lys Gln Ile Val Glu Thr Asp 
                245                 250                 255     


Thr Asn Glu Asn Phe Glu Gly Val Ser Phe Met Gly Lys Ile Cys Gly 
            260                 265                 270         


Val Ser Ile Val Arg Ala Gly Glu Ser Met Glu Gln Gly Leu Arg Asp 
        275                 280                 285             


Cys Cys Arg Ser Val Arg Ile Gly Lys Ile Leu Ile Gln Arg Asp Glu 
    290                 295                 300                 


Glu Thr Ala Leu Pro Lys Leu Phe Tyr Glu Lys Leu Pro Glu Asp Ile 
305                 310                 315                 320 


Ser Glu Arg Tyr Val Phe Leu Leu Asp Pro Met Leu Ala Thr Gly Gly 
                325                 330                 335     


Ser Ala Ile Met Ala Thr Glu Val Leu Ile Lys Arg Gly Val Lys Pro 
            340                 345                 350         


Glu Arg Ile Tyr Phe Leu Asn Leu Ile Cys Ser Lys Glu Gly Ile Glu 
        355                 360                 365             


Lys Tyr His Ala Ala Phe Pro Glu Val Arg Ile Val Thr Gly Ala Leu 
    370                 375                 380                 


Asp Arg Gly Leu Asp Glu Asn Lys Tyr Leu Val Pro Gly Leu Gly Asp 
385                 390                 395                 400 


Phe Gly Asp Arg Tyr Tyr Cys Val 
                405             


<210>  13
<211>  1287
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion construction - CDopt - linker - UPRT


<220>
<221>  CDS
<222>  (1)..(1287)

<400>  13
atg gtg acc ggc ggc atg gcc tcc aag tgg gat caa aag ggc atg gat         48
Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp           
1               5                   10                  15                

atc gct tac gag gag gcc ctg ctg ggc tac aag gag ggc ggc gtg cct         96
Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro           
            20                  25                  30                    

atc ggc ggc tgt ctg atc aac aac aag gac ggc agt gtg ctg ggc agg        144
Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg           
        35                  40                  45                        

ggc cac aac atg agg ttc cag aag ggc tcc gcc acc ctg cac ggc gag        192
Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu           
    50                  55                  60                            

atc tcc acc ctg gag aac tgt ggc agg ctg gag ggc aag gtg tac aag        240
Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys           
65                  70                  75                  80            

gac acc acc ctg tac acc acc ctg tcc cct tgt gac atg tgt acc ggc        288
Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly           
                85                  90                  95                

gct atc atc atg tac ggc atc cct agg tgt gtg atc ggc gag aac gtg        336
Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val           
            100                 105                 110                   

aac ttc aag tcc aag ggc gag aag tac ctg caa acc agg ggc cac gag        384
Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu           
        115                 120                 125                       

gtg gtg gtt gtt gac gat gag agg tgt aag aag ctg atg aag cag ttc        432
Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe           
    130                 135                 140                           

atc gac gag agg cct cag gac tgg ttc gag gat atc ggc gag tcc ggc        480
Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu Ser Gly           
145                 150                 155                 160           

ggc ggc gcc tcc ggc ggc ggc gcc tcc ggc ggc ggc gcc tcc ggc ggc        528
Gly Gly Ala Ser Gly Gly Gly Ala Ser Gly Gly Gly Ala Ser Gly Gly           
                165                 170                 175               

ggc gcc aac ccg tta ttc ttt ttg gct tct cca ttc ttg tac ctt aca        576
Gly Ala Asn Pro Leu Phe Phe Leu Ala Ser Pro Phe Leu Tyr Leu Thr           
            180                 185                 190                   

tat ctt ata tat tat cca aac aaa ggg tct ttc gtt agc aaa cct aga        624
Tyr Leu Ile Tyr Tyr Pro Asn Lys Gly Ser Phe Val Ser Lys Pro Arg           
        195                 200                 205                       

aat ctg caa aaa atg tct tcg gaa cca ttt aag aac gtc tac ttg cta        672
Asn Leu Gln Lys Met Ser Ser Glu Pro Phe Lys Asn Val Tyr Leu Leu           
    210                 215                 220                           

cct caa aca aac caa ttg ctg ggt ttg tac acc atc atc aga aat aag        720
Pro Gln Thr Asn Gln Leu Leu Gly Leu Tyr Thr Ile Ile Arg Asn Lys           
225                 230                 235                 240           

aat aca act aga cct gat ttc att ttc tac tcc gat aga atc atc aga        768
Asn Thr Thr Arg Pro Asp Phe Ile Phe Tyr Ser Asp Arg Ile Ile Arg           
                245                 250                 255               

ttg ttg gtt gaa gaa ggt ttg aac cat cta cct gtg caa aag caa att        816
Leu Leu Val Glu Glu Gly Leu Asn His Leu Pro Val Gln Lys Gln Ile           
            260                 265                 270                   

gtg gaa act gac acc aac gaa aac ttc gaa ggt gtc tca ttc atg ggt        864
Val Glu Thr Asp Thr Asn Glu Asn Phe Glu Gly Val Ser Phe Met Gly           
        275                 280                 285                       

aaa atc tgt ggt gtt tcc att gtc aga gct ggt gaa tcg atg gag caa        912
Lys Ile Cys Gly Val Ser Ile Val Arg Ala Gly Glu Ser Met Glu Gln           
    290                 295                 300                           

gga tta aga gac tgt tgt agg tct gtg cgt atc ggt aaa att tta att        960
Gly Leu Arg Asp Cys Cys Arg Ser Val Arg Ile Gly Lys Ile Leu Ile           
305                 310                 315                 320           

caa agg gac gag gag act gct tta cca aag tta ttc tac gaa aaa tta       1008
Gln Arg Asp Glu Glu Thr Ala Leu Pro Lys Leu Phe Tyr Glu Lys Leu           
                325                 330                 335               

cca gag gat ata tct gaa agg tat gtc ttc cta tta gac cca atg ctg       1056
Pro Glu Asp Ile Ser Glu Arg Tyr Val Phe Leu Leu Asp Pro Met Leu           
            340                 345                 350                   

gcc acc ggt ggt agt gct atc atg gct aca gaa gtc ttg att aag aga       1104
Ala Thr Gly Gly Ser Ala Ile Met Ala Thr Glu Val Leu Ile Lys Arg           
        355                 360                 365                       

ggt gtt aag cca gag aga att tac ttc tta aac cta atc tgt agt aag       1152
Gly Val Lys Pro Glu Arg Ile Tyr Phe Leu Asn Leu Ile Cys Ser Lys           
    370                 375                 380                           

gaa ggg att gaa aaa tac cat gcc gcc ttc cca gag gtc aga att gtt       1200
Glu Gly Ile Glu Lys Tyr His Ala Ala Phe Pro Glu Val Arg Ile Val           
385                 390                 395                 400           

act ggt gcc ctc gac aga ggt cta gat gaa aac aag tat cta gtt cca       1248
Thr Gly Ala Leu Asp Arg Gly Leu Asp Glu Asn Lys Tyr Leu Val Pro           
                405                 410                 415               

ggg ttg ggt gac ttt ggt gac aga tac tac tgt gtt taa                   1287
Gly Leu Gly Asp Phe Gly Asp Arg Tyr Tyr Cys Val                           
            420                 425                                       


<210>  14
<211>  428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp 
1               5                   10                  15      


Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro 
            20                  25                  30          


Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg 
        35                  40                  45              


Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu 
    50                  55                  60                  


Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys 
65                  70                  75                  80  


Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly 
                85                  90                  95      


Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val 
            100                 105                 110         


Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu 
        115                 120                 125             


Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe 
    130                 135                 140                 


Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu Ser Gly 
145                 150                 155                 160 


Gly Gly Ala Ser Gly Gly Gly Ala Ser Gly Gly Gly Ala Ser Gly Gly 
                165                 170                 175     


Gly Ala Asn Pro Leu Phe Phe Leu Ala Ser Pro Phe Leu Tyr Leu Thr 
            180                 185                 190         


Tyr Leu Ile Tyr Tyr Pro Asn Lys Gly Ser Phe Val Ser Lys Pro Arg 
        195                 200                 205             


Asn Leu Gln Lys Met Ser Ser Glu Pro Phe Lys Asn Val Tyr Leu Leu 
    210                 215                 220                 


Pro Gln Thr Asn Gln Leu Leu Gly Leu Tyr Thr Ile Ile Arg Asn Lys 
225                 230                 235                 240 


Asn Thr Thr Arg Pro Asp Phe Ile Phe Tyr Ser Asp Arg Ile Ile Arg 
                245                 250                 255     


Leu Leu Val Glu Glu Gly Leu Asn His Leu Pro Val Gln Lys Gln Ile 
            260                 265                 270         


Val Glu Thr Asp Thr Asn Glu Asn Phe Glu Gly Val Ser Phe Met Gly 
        275                 280                 285             


Lys Ile Cys Gly Val Ser Ile Val Arg Ala Gly Glu Ser Met Glu Gln 
    290                 295                 300                 


Gly Leu Arg Asp Cys Cys Arg Ser Val Arg Ile Gly Lys Ile Leu Ile 
305                 310                 315                 320 


Gln Arg Asp Glu Glu Thr Ala Leu Pro Lys Leu Phe Tyr Glu Lys Leu 
                325                 330                 335     


Pro Glu Asp Ile Ser Glu Arg Tyr Val Phe Leu Leu Asp Pro Met Leu 
            340                 345                 350         


Ala Thr Gly Gly Ser Ala Ile Met Ala Thr Glu Val Leu Ile Lys Arg 
        355                 360                 365             


Gly Val Lys Pro Glu Arg Ile Tyr Phe Leu Asn Leu Ile Cys Ser Lys 
    370                 375                 380                 


Glu Gly Ile Glu Lys Tyr His Ala Ala Phe Pro Glu Val Arg Ile Val 
385                 390                 395                 400 


Thr Gly Ala Leu Asp Arg Gly Leu Asp Glu Asn Lys Tyr Leu Val Pro 
                405                 410                 415     


Gly Leu Gly Asp Phe Gly Asp Arg Tyr Tyr Cys Val 
            420                 425             


<210>  15
<211>  1200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion Construct - CDopt3 - OPRT


<220>
<221>  CDS
<222>  (1)..(1200)

<400>  15
atg gtg acc ggc ggc atg gcc tcc aag tgg gat caa aag ggc atg gat         48
Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp           
1               5                   10                  15                

atc gct tac gag gag gcc ctg ctg ggc tac aag gag ggc ggc gtg cct         96
Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro           
            20                  25                  30                    

atc ggc ggc tgt ctg atc aac aac aag gac ggc agt gtg ctg ggc agg        144
Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg           
        35                  40                  45                        

ggc cac aac atg agg ttc cag aag ggc tcc gcc acc ctg cac ggc gag        192
Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu           
    50                  55                  60                            

atc tcc acc ctg gag aac tgt ggc agg ctg gag ggc aag gtg tac aag        240
Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys           
65                  70                  75                  80            

gac acc acc ctg tac acc acc ctg tcc cct tgt gac atg tgt acc ggc        288
Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly           
                85                  90                  95                

gct atc atc atg tac ggc atc cct agg tgt gtg atc ggc gag aac gtg        336
Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val           
            100                 105                 110                   

aac ttc aag tcc aag ggc gag aag tac ctg caa acc agg ggc cac gag        384
Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu           
        115                 120                 125                       

gtg gtg gtt gtt gac gat gag agg tgt aag aag ctg atg aag cag ttc        432
Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe           
    130                 135                 140                           

atc gac gag agg cct cag gac tgg ttc gag gat atc ggc gag gcg gtc        480
Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu Ala Val           
145                 150                 155                 160           

gct cgt gca gct ttg ggg cca ttg gtg acg ggt ctg tac gac gtg cag        528
Ala Arg Ala Ala Leu Gly Pro Leu Val Thr Gly Leu Tyr Asp Val Gln           
                165                 170                 175               

gct ttc aag ttt ggg gac ttc gtg ctg aag agc ggg ctt tcc tcc ccc        576
Ala Phe Lys Phe Gly Asp Phe Val Leu Lys Ser Gly Leu Ser Ser Pro           
            180                 185                 190                   

atc tac atc gat ctg cgg ggc atc gtg tct cga ccg cgt ctt ctg agt        624
Ile Tyr Ile Asp Leu Arg Gly Ile Val Ser Arg Pro Arg Leu Leu Ser           
        195                 200                 205                       

cag gtt gca gat att tta ttc caa act gcc caa aat gca ggc atc agt        672
Gln Val Ala Asp Ile Leu Phe Gln Thr Ala Gln Asn Ala Gly Ile Ser           
    210                 215                 220                           

ttt gac acc gtg tgt gga gtg cct tat aca gct ttg cca ttg gct aca        720
Phe Asp Thr Val Cys Gly Val Pro Tyr Thr Ala Leu Pro Leu Ala Thr           
225                 230                 235                 240           

gtt atc tgt tca acc aat caa att cca atg ctt att aga agg aaa gaa        768
Val Ile Cys Ser Thr Asn Gln Ile Pro Met Leu Ile Arg Arg Lys Glu           
                245                 250                 255               

aca aag gat tat gga act aag cgt ctt gta gaa gga act att aat cca        816
Thr Lys Asp Tyr Gly Thr Lys Arg Leu Val Glu Gly Thr Ile Asn Pro           
            260                 265                 270                   

gga gaa acc tgt tta atc att gaa gat gtt gtc acc agt gga tct agt        864
Gly Glu Thr Cys Leu Ile Ile Glu Asp Val Val Thr Ser Gly Ser Ser           
        275                 280                 285                       

gtt ttg gaa act gtt gag gtt ctt cag aag gag ggc ttg aag gtc act        912
Val Leu Glu Thr Val Glu Val Leu Gln Lys Glu Gly Leu Lys Val Thr           
    290                 295                 300                           

gat gcc ata gtg ctg ttg gac aga gag cag gga ggc aag gac aag ttg        960
Asp Ala Ile Val Leu Leu Asp Arg Glu Gln Gly Gly Lys Asp Lys Leu           
305                 310                 315                 320           

cag gcg cac ggg atc cgc ctc cac tca gtg tgt aca ttg tcc aaa atg       1008
Gln Ala His Gly Ile Arg Leu His Ser Val Cys Thr Leu Ser Lys Met           
                325                 330                 335               

ctg gag att ctc gag cag cag aaa aaa gtt gat gct gag aca gtt ggg       1056
Leu Glu Ile Leu Glu Gln Gln Lys Lys Val Asp Ala Glu Thr Val Gly           
            340                 345                 350                   

aga gtg aag agg ttt att cag gag aat gtc ttt gtg gca gcg aat cat       1104
Arg Val Lys Arg Phe Ile Gln Glu Asn Val Phe Val Ala Ala Asn His           
        355                 360                 365                       

aat ggt tct ccc ctt tct ata aag gaa gca ccc aaa gaa ctc agc ttc       1152
Asn Gly Ser Pro Leu Ser Ile Lys Glu Ala Pro Lys Glu Leu Ser Phe           
    370                 375                 380                           

ggt gca cgt gca gag ctg ccc agg atc cac cca gtt gca tcg aag taa       1200
Gly Ala Arg Ala Glu Leu Pro Arg Ile His Pro Val Ala Ser Lys               
385                 390                 395                               


<210>  16
<211>  399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp 
1               5                   10                  15      


Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro 
            20                  25                  30          


Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg 
        35                  40                  45              


Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu 
    50                  55                  60                  


Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys 
65                  70                  75                  80  


Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly 
                85                  90                  95      


Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val 
            100                 105                 110         


Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu 
        115                 120                 125             


Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe 
    130                 135                 140                 


Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu Ala Val 
145                 150                 155                 160 


Ala Arg Ala Ala Leu Gly Pro Leu Val Thr Gly Leu Tyr Asp Val Gln 
                165                 170                 175     


Ala Phe Lys Phe Gly Asp Phe Val Leu Lys Ser Gly Leu Ser Ser Pro 
            180                 185                 190         


Ile Tyr Ile Asp Leu Arg Gly Ile Val Ser Arg Pro Arg Leu Leu Ser 
        195                 200                 205             


Gln Val Ala Asp Ile Leu Phe Gln Thr Ala Gln Asn Ala Gly Ile Ser 
    210                 215                 220                 


Phe Asp Thr Val Cys Gly Val Pro Tyr Thr Ala Leu Pro Leu Ala Thr 
225                 230                 235                 240 


Val Ile Cys Ser Thr Asn Gln Ile Pro Met Leu Ile Arg Arg Lys Glu 
                245                 250                 255     


Thr Lys Asp Tyr Gly Thr Lys Arg Leu Val Glu Gly Thr Ile Asn Pro 
            260                 265                 270         


Gly Glu Thr Cys Leu Ile Ile Glu Asp Val Val Thr Ser Gly Ser Ser 
        275                 280                 285             


Val Leu Glu Thr Val Glu Val Leu Gln Lys Glu Gly Leu Lys Val Thr 
    290                 295                 300                 


Asp Ala Ile Val Leu Leu Asp Arg Glu Gln Gly Gly Lys Asp Lys Leu 
305                 310                 315                 320 


Gln Ala His Gly Ile Arg Leu His Ser Val Cys Thr Leu Ser Lys Met 
                325                 330                 335     


Leu Glu Ile Leu Glu Gln Gln Lys Lys Val Asp Ala Glu Thr Val Gly 
            340                 345                 350         


Arg Val Lys Arg Phe Ile Gln Glu Asn Val Phe Val Ala Ala Asn His 
        355                 360                 365             


Asn Gly Ser Pro Leu Ser Ile Lys Glu Ala Pro Lys Glu Leu Ser Phe 
    370                 375                 380                 


Gly Ala Arg Ala Glu Leu Pro Arg Ile His Pro Val Ala Ser Lys 
385                 390                 395                 


<210>  17
<211>  1260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion Construct - CDopt3 - linker - OPRT


<220>
<221>  CDS
<222>  (1)..(1260)

<400>  17
atg gtg acc ggc ggc atg gcc tcc aag tgg gat caa aag ggc atg gat         48
Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp           
1               5                   10                  15                

atc gct tac gag gag gcc ctg ctg ggc tac aag gag ggc ggc gtg cct         96
Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro           
            20                  25                  30                    

atc ggc ggc tgt ctg atc aac aac aag gac ggc agt gtg ctg ggc agg        144
Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg           
        35                  40                  45                        

ggc cac aac atg agg ttc cag aag ggc tcc gcc acc ctg cac ggc gag        192
Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu           
    50                  55                  60                            

atc tcc acc ctg gag aac tgt ggc agg ctg gag ggc aag gtg tac aag        240
Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys           
65                  70                  75                  80            

gac acc acc ctg tac acc acc ctg tcc cct tgt gac atg tgt acc ggc        288
Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly           
                85                  90                  95                

gct atc atc atg tac ggc atc cct agg tgt gtg atc ggc gag aac gtg        336
Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val           
            100                 105                 110                   

aac ttc aag tcc aag ggc gag aag tac ctg caa acc agg ggc cac gag        384
Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu           
        115                 120                 125                       

gtg gtg gtt gtt gac gat gag agg tgt aag aag ctg atg aag cag ttc        432
Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe           
    130                 135                 140                           

atc gac gag agg cct cag gac tgg ttc gag gat atc ggc gag tcc ggc        480
Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu Ser Gly           
145                 150                 155                 160           

ggc ggc gcc tcc ggc ggc ggc gcc tcc ggc ggc ggc gcc tcc ggc ggc        528
Gly Gly Ala Ser Gly Gly Gly Ala Ser Gly Gly Gly Ala Ser Gly Gly           
                165                 170                 175               

ggc gcc gcg gtc gct cgt gca gct ttg ggg cca ttg gtg acg ggt ctg        576
Gly Ala Ala Val Ala Arg Ala Ala Leu Gly Pro Leu Val Thr Gly Leu           
            180                 185                 190                   

tac gac gtg cag gct ttc aag ttt ggg gac ttc gtg ctg aag agc ggg        624
Tyr Asp Val Gln Ala Phe Lys Phe Gly Asp Phe Val Leu Lys Ser Gly           
        195                 200                 205                       

ctt tcc tcc ccc atc tac atc gat ctg cgg ggc atc gtg tct cga ccg        672
Leu Ser Ser Pro Ile Tyr Ile Asp Leu Arg Gly Ile Val Ser Arg Pro           
    210                 215                 220                           

cgt ctt ctg agt cag gtt gca gat att tta ttc caa act gcc caa aat        720
Arg Leu Leu Ser Gln Val Ala Asp Ile Leu Phe Gln Thr Ala Gln Asn           
225                 230                 235                 240           

gca ggc atc agt ttt gac acc gtg tgt gga gtg cct tat aca gct ttg        768
Ala Gly Ile Ser Phe Asp Thr Val Cys Gly Val Pro Tyr Thr Ala Leu           
                245                 250                 255               

cca ttg gct aca gtt atc tgt tca acc aat caa att cca atg ctt att        816
Pro Leu Ala Thr Val Ile Cys Ser Thr Asn Gln Ile Pro Met Leu Ile           
            260                 265                 270                   

aga agg aaa gaa aca aag gat tat gga act aag cgt ctt gta gaa gga        864
Arg Arg Lys Glu Thr Lys Asp Tyr Gly Thr Lys Arg Leu Val Glu Gly           
        275                 280                 285                       

act att aat cca gga gaa acc tgt tta atc att gaa gat gtt gtc acc        912
Thr Ile Asn Pro Gly Glu Thr Cys Leu Ile Ile Glu Asp Val Val Thr           
    290                 295                 300                           

agt gga tct agt gtt ttg gaa act gtt gag gtt ctt cag aag gag ggc        960
Ser Gly Ser Ser Val Leu Glu Thr Val Glu Val Leu Gln Lys Glu Gly           
305                 310                 315                 320           

ttg aag gtc act gat gcc ata gtg ctg ttg gac aga gag cag gga ggc       1008
Leu Lys Val Thr Asp Ala Ile Val Leu Leu Asp Arg Glu Gln Gly Gly           
                325                 330                 335               

aag gac aag ttg cag gcg cac ggg atc cgc ctc cac tca gtg tgt aca       1056
Lys Asp Lys Leu Gln Ala His Gly Ile Arg Leu His Ser Val Cys Thr           
            340                 345                 350                   

ttg tcc aaa atg ctg gag att ctc gag cag cag aaa aaa gtt gat gct       1104
Leu Ser Lys Met Leu Glu Ile Leu Glu Gln Gln Lys Lys Val Asp Ala           
        355                 360                 365                       

gag aca gtt ggg aga gtg aag agg ttt att cag gag aat gtc ttt gtg       1152
Glu Thr Val Gly Arg Val Lys Arg Phe Ile Gln Glu Asn Val Phe Val           
    370                 375                 380                           

gca gcg aat cat aat ggt tct ccc ctt tct ata aag gaa gca ccc aaa       1200
Ala Ala Asn His Asn Gly Ser Pro Leu Ser Ile Lys Glu Ala Pro Lys           
385                 390                 395                 400           

gaa ctc agc ttc ggt gca cgt gca gag ctg ccc agg atc cac cca gtt       1248
Glu Leu Ser Phe Gly Ala Arg Ala Glu Leu Pro Arg Ile His Pro Val           
                405                 410                 415               

gca tcg aag taa                                                       1260
Ala Ser Lys                                                               
                                                                          


<210>  18
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  18

Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp 
1               5                   10                  15      


Ile Ala Tyr Glu Glu Ala Leu Leu Gly Tyr Lys Glu Gly Gly Val Pro 
            20                  25                  30          


Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg 
        35                  40                  45              


Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu 
    50                  55                  60                  


Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys 
65                  70                  75                  80  


Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly 
                85                  90                  95      


Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Ile Gly Glu Asn Val 
            100                 105                 110         


Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu 
        115                 120                 125             


Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Leu Met Lys Gln Phe 
    130                 135                 140                 


Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu Ser Gly 
145                 150                 155                 160 


Gly Gly Ala Ser Gly Gly Gly Ala Ser Gly Gly Gly Ala Ser Gly Gly 
                165                 170                 175     


Gly Ala Ala Val Ala Arg Ala Ala Leu Gly Pro Leu Val Thr Gly Leu 
            180                 185                 190         


Tyr Asp Val Gln Ala Phe Lys Phe Gly Asp Phe Val Leu Lys Ser Gly 
        195                 200                 205             


Leu Ser Ser Pro Ile Tyr Ile Asp Leu Arg Gly Ile Val Ser Arg Pro 
    210                 215                 220                 


Arg Leu Leu Ser Gln Val Ala Asp Ile Leu Phe Gln Thr Ala Gln Asn 
225                 230                 235                 240 


Ala Gly Ile Ser Phe Asp Thr Val Cys Gly Val Pro Tyr Thr Ala Leu 
                245                 250                 255     


Pro Leu Ala Thr Val Ile Cys Ser Thr Asn Gln Ile Pro Met Leu Ile 
            260                 265                 270         


Arg Arg Lys Glu Thr Lys Asp Tyr Gly Thr Lys Arg Leu Val Glu Gly 
        275                 280                 285             


Thr Ile Asn Pro Gly Glu Thr Cys Leu Ile Ile Glu Asp Val Val Thr 
    290                 295                 300                 


Ser Gly Ser Ser Val Leu Glu Thr Val Glu Val Leu Gln Lys Glu Gly 
305                 310                 315                 320 


Leu Lys Val Thr Asp Ala Ile Val Leu Leu Asp Arg Glu Gln Gly Gly 
                325                 330                 335     


Lys Asp Lys Leu Gln Ala His Gly Ile Arg Leu His Ser Val Cys Thr 
            340                 345                 350         


Leu Ser Lys Met Leu Glu Ile Leu Glu Gln Gln Lys Lys Val Asp Ala 
        355                 360                 365             


Glu Thr Val Gly Arg Val Lys Arg Phe Ile Gln Glu Asn Val Phe Val 
    370                 375                 380                 


Ala Ala Asn His Asn Gly Ser Pro Leu Ser Ile Lys Glu Ala Pro Lys 
385                 390                 395                 400 


Glu Leu Ser Phe Gly Ala Arg Ala Glu Leu Pro Arg Ile His Pro Val 
                405                 410                 415     


Ala Ser Lys 
            


<210>  19
<211>  11892
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RCR Vector - pAC3-yCD2

<400>  19
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaga     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgttc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag catcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgtt actggccgaa     8340

gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt     8400

cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg     8460

gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc     8520

ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc     8580

ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa     8640

aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc     8700

tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg     8760

gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac     8820

gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga ttataaatgg     8880

tgaccggcgg catggcctcc aagtgggatc aaaagggcat ggatatcgct tacgaggagg     8940

ccctgctggg ctacaaggag ggcggcgtgc ctatcggcgg ctgtctgatc aacaacaagg     9000

acggcagtgt gctgggcagg ggccacaaca tgaggttcca gaagggctcc gccaccctgc     9060

acggcgagat ctccaccctg gagaactgtg gcaggctgga gggcaaggtg tacaaggaca     9120

ccaccctgta caccaccctg tccccttgtg acatgtgtac cggcgctatc atcatgtacg     9180

gcatccctag gtgtgtgatc ggcgagaacg tgaacttcaa gtccaagggc gagaagtacc     9240

tgcaaaccag gggccacgag gtggtggttg ttgacgatga gaggtgtaag aagctgatga     9300

agcagttcat cgacgagagg cctcaggact ggttcgagga tatcggcgag taagcggccg     9360

cagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc     9420

tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa     9480

ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca     9540

aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca     9600

gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     9660

agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg     9720

tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag     9780

ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa     9840

cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca     9900

ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct     9960

ctgagtgatt gactacccgt cagcgggggt ctttcattac atgtgagcaa aaggccagca    10020

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc    10080

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata    10140

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc    10200

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc    10260

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga    10320

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc    10380

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag    10440

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag    10500

gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag    10560

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca    10620

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga    10680

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat    10740

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga    10800

gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg    10860

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga    10920

gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc    10980

agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac    11040

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc    11100

agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc    11160

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc    11220

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt    11280

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc    11340

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg    11400

tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag    11460

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat    11520

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc    11580

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa    11640

aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta    11700

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa    11760

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga    11820

aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct    11880

tcaagaattc at                                                        11892


<210>  20
<211>  11892
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RCR Vector - pAC3-yCD

<400>  20
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaga     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgttc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag catcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgtt actggccgaa     8340

gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt     8400

cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg     8460

gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc     8520

ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc     8580

ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa     8640

aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc     8700

tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg     8760

gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac     8820

gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga ttataaatgg     8880

tgacaggggg aatggcaagc aagtgggatc agaagggtat ggacattgcc tatgaggagg     8940

cggccttagg ttacaaagag ggtggtgttc ctattggcgg atgtcttatc aataacaaag     9000

acggaagtgt tctcggtcgt ggtcacaaca tgagatttca aaagggatcc gccacactac     9060

atggtgagat ctccactttg gaaaactgtg ggagattaga gggcaaagtg tacaaagata     9120

ccactttgta tacgacgctg tctccatgcg acatgtgtac aggtgccatc atcatgtatg     9180

gtattccacg ctgtgttgtc ggtgagaacg ttaatttcaa aagtaagggc gagaaatatt     9240

tacaaactag aggtcacgag gttgttgttg ttgacgatga gaggtgtaaa aagatcatga     9300

aacaatttat cgatgaaaga cctcaggatt ggtttgaaga tattggtgag taggcggccg     9360

cagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc     9420

tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa     9480

ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca     9540

aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca     9600

gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     9660

agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg     9720

tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag     9780

ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa     9840

cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca     9900

ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct     9960

ctgagtgatt gactacccgt cagcgggggt ctttcattac atgtgagcaa aaggccagca    10020

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc    10080

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata    10140

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc    10200

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc    10260

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga    10320

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc    10380

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag    10440

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag    10500

gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag    10560

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca    10620

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga    10680

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat    10740

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga    10800

gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg    10860

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga    10920

gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc    10980

agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac    11040

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc    11100

agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc    11160

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc    11220

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt    11280

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc    11340

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg    11400

tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag    11460

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat    11520

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc    11580

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa    11640

aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta    11700

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa    11760

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga    11820

aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct    11880

tcaagaattc at                                                        11892


<210>  21
<211>  12007
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RCR Vector - pACE-CD

<400>  21
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaga     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgttc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag catcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac     8280

tcaacaatat caccagctga agcctataga gtacgagcca tgacgtacgt tactggccga     8340

agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg     8400

tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg     8460

ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt     8520

cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac     8580

cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca     8640

aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg     8700

ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg     8760

ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa     8820

cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat     8880

ggtgacaggg ggaatggcaa gcaagtggga tcagaagggt atggacattg cctatgagga     8940

ggcggcctta ggttacaaag agggtggtgt tcctattggc ggatgtctta tcaataacaa     9000

agacggaagt gttctcggtc gtggtcacaa catgagattt caaaagggat ccgccacact     9060

acatggtgag atctccactt tggaaaactg tgggagatta gagggcaaag tgtacaaaga     9120

taccactttg tatacgacgc tgtctccatg cgacatgtgt acaggtgcca tcatcatgta     9180

tggtattcca cgctgtgttg tcggtgagaa cgttaatttc aaaagtaagg gcgagaaata     9240

tttacaaact agaggtcacg aggttgttgt tgttgacgat gagaggtgta aaaagatcat     9300

gaaacaattt atcgatgaaa gacctcagga ttggtttgaa gatattggtg agtaggcggc     9360

cgcgccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga     9420

ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa     9480

tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata     9540

tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga     9600

tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc     9660

agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca     9720

tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttgt ttaaactaac     9780

caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag     9840

cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt     9900

gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg     9960

gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg    10020

gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct    10080

cgcgcgtttc ggtgatgacg gtgaaaacct ctgacatgtg agcaaaaggc cagcaaaagg    10140

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg    10200

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat    10260

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta    10320

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct    10380

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc    10440

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa    10500

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg    10560

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag    10620

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt    10680

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta    10740

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc    10800

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca    10860

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa    10920

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat    10980

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct    11040

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt    11100

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat    11160

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta    11220

atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg    11280

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt    11340

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg    11400

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg    11460

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc    11520

ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa    11580

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac    11640

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt    11700

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg    11760

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa    11820

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata    11880

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca    11940

ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag    12000

aattcat                                                              12007


<210>  22
<211>  11893
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RCR Vector - pAC3-yCD2

<400>  22
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgtt actggccgaa     8340

gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt     8400

cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg     8460

gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc     8520

ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc     8580

ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa     8640

aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc     8700

tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg     8760

gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac     8820

gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga ttataaatgg     8880

tgaccggcgg catggcctcc aagtgggatc aaaagggcat ggatatcgct tacgaggagg     8940

ccctgctggg ctacaaggag ggcggcgtgc ctatcggcgg ctgtctgatc aacaacaagg     9000

acggcagtgt gctgggcagg ggccacaaca tgaggttcca gaagggctcc gccaccctgc     9060

acggcgagat ctccaccctg gagaactgtg gcaggctgga gggcaaggtg tacaaggaca     9120

ccaccctgta caccaccctg tccccttgtg acatgtgtac cggcgctatc atcatgtacg     9180

gcatccctag gtgtgtgatc ggcgagaacg tgaacttcaa gtccaagggc gagaagtacc     9240

tgcaaaccag gggccacgag gtggtggttg ttgacgatga gaggtgtaag aagctgatga     9300

agcagttcat cgacgagagg cctcaggact ggttcgagga tatcggcgag taagcggccg     9360

cagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc     9420

tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa     9480

ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca     9540

aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca     9600

gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     9660

agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg     9720

tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag     9780

ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa     9840

cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca     9900

ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct     9960

ctgagtgatt gactacccgt cagcgggggt ctttcattac atgtgagcaa aaggccagca    10020

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc    10080

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata    10140

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc    10200

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc    10260

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga    10320

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc    10380

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag    10440

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag    10500

gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag    10560

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca    10620

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga    10680

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat    10740

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga    10800

gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg    10860

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga    10920

gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc    10980

agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac    11040

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc    11100

agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc    11160

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc    11220

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt    11280

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc    11340

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg    11400

tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag    11460

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat    11520

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc    11580

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa    11640

aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta    11700

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa    11760

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga    11820

aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct    11880

tcaagaattc cat                                                       11893


