                         SEQUENCE LISTING

<110>  The Regents of the University of California
       Chen, Wilfred
       Tsai, Shen-Long
 
<120>  ENGINEERED YEAST FOR CELLULOSIC ETHANOL PRODUCTION

<130>  00013-024WO1

<140>  Not yet known
<141>  2009-11-13

<150>  US 61/115,068
<151>  2008-11-15

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  2052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant scaffoldin polypeptide


<220>
<221>  CDS
<222>  (1)..(2052)

<400>  1
atg ggc gat tct ctt aaa gtt aca gta gga aca gct aat ggt aag cct         48
Met Gly Asp Ser Leu Lys Val Thr Val Gly Thr Ala Asn Gly Lys Pro           
1               5                   10                  15                

ggc gat aca gta aca gtt cct gtt aca ttt gct gat gta gca aag atg         96
Gly Asp Thr Val Thr Val Pro Val Thr Phe Ala Asp Val Ala Lys Met           
            20                  25                  30                    

aaa aac gta gga aca tgt aat ttc tat ctt gga tat gat gca agc ctg        144
Lys Asn Val Gly Thr Cys Asn Phe Tyr Leu Gly Tyr Asp Ala Ser Leu           
        35                  40                  45                        

tta gag gta gta tca gta gat gca ggt cca ata gtt aag aat gca gca        192
Leu Glu Val Val Ser Val Asp Ala Gly Pro Ile Val Lys Asn Ala Ala           
    50                  55                  60                            

gtt aac ttc tca agc agt gca agc aac gga aca atc agc ttc ctg ttc        240
Val Asn Phe Ser Ser Ser Ala Ser Asn Gly Thr Ile Ser Phe Leu Phe           
65                  70                  75                  80            

ttg gat aac aca att aca gac gaa ttg ata act gca gac ggt gtg ttt        288
Leu Asp Asn Thr Ile Thr Asp Glu Leu Ile Thr Ala Asp Gly Val Phe           
                85                  90                  95                

gca aat att aag ttc aaa tta aag agt gta acg gct aaa act aca aca        336
Ala Asn Ile Lys Phe Lys Leu Lys Ser Val Thr Ala Lys Thr Thr Thr           
            100                 105                 110                   

cca gta aca ttt aaa gat ggt gga gct ttt ggt gac gga act atg tca        384
Pro Val Thr Phe Lys Asp Gly Gly Ala Phe Gly Asp Gly Thr Met Ser           
        115                 120                 125                       

aag ata gct tca gtt act aag aca aac ggt agt gta acg atc gat ccg        432
Lys Ile Ala Ser Val Thr Lys Thr Asn Gly Ser Val Thr Ile Asp Pro           
    130                 135                 140                           

acc aag gga gca aca cca aca aat aca gct acg ccg aca aaa tca gct        480
Thr Lys Gly Ala Thr Pro Thr Asn Thr Ala Thr Pro Thr Lys Ser Ala           
145                 150                 155                 160           

acg gct acg ccc acc agg cca tcg gta ccg aca aac aca ccg aca aac        528
Thr Ala Thr Pro Thr Arg Pro Ser Val Pro Thr Asn Thr Pro Thr Asn           
                165                 170                 175               

aca ccg gca aat aca ccg gta tca ggc aat ttg aag gtt gaa ttc tac        576
Thr Pro Ala Asn Thr Pro Val Ser Gly Asn Leu Lys Val Glu Phe Tyr           
            180                 185                 190                   

aac agc aat cct tca gat act act aac tca atc aat cct cag ttc aag        624
Asn Ser Asn Pro Ser Asp Thr Thr Asn Ser Ile Asn Pro Gln Phe Lys           
        195                 200                 205                       

gtt act aat acc gga agc agt gca att gat ttg tcc aaa ctc aca ttg        672
Val Thr Asn Thr Gly Ser Ser Ala Ile Asp Leu Ser Lys Leu Thr Leu           
    210                 215                 220                           

aga tat tat tat aca gta gac gga cag aaa gat cag acc ttc tgg tgt        720
Arg Tyr Tyr Tyr Thr Val Asp Gly Gln Lys Asp Gln Thr Phe Trp Cys           
225                 230                 235                 240           

gac cat gct gca ata atc ggc agt aac ggc agc tac aac gga att act        768
Asp His Ala Ala Ile Ile Gly Ser Asn Gly Ser Tyr Asn Gly Ile Thr           
                245                 250                 255               

tca aat gta aaa gga aca ttt gta aaa atg agt tcc tca aca aat aac        816
Ser Asn Val Lys Gly Thr Phe Val Lys Met Ser Ser Ser Thr Asn Asn           
            260                 265                 270                   

gca gac acc tac ctt gaa ata agc ttt aca ggc gga act ctt gaa ccg        864
Ala Asp Thr Tyr Leu Glu Ile Ser Phe Thr Gly Gly Thr Leu Glu Pro           
        275                 280                 285                       

ggt gca cat gtt cag ata caa ggt aga ttt gca aag aat gac tgg agt        912
Gly Ala His Val Gln Ile Gln Gly Arg Phe Ala Lys Asn Asp Trp Ser           
    290                 295                 300                           

aac tat aca cag tca aat gac tac tca ttc aag tct gct tca cag ttt        960
Asn Tyr Thr Gln Ser Asn Asp Tyr Ser Phe Lys Ser Ala Ser Gln Phe           
305                 310                 315                 320           

gtt gaa tgg gat cag gta aca gca tac ttg aac ggt gtt ctt gta tgg       1008
Val Glu Trp Asp Gln Val Thr Ala Tyr Leu Asn Gly Val Leu Val Trp           
                325                 330                 335               

ggt aaa gaa ccc ggt ggc agt gta gta cca tca aca cag cct gta aca       1056
Gly Lys Glu Pro Gly Gly Ser Val Val Pro Ser Thr Gln Pro Val Thr           
            340                 345                 350                   

aca cca cct gca aca aca aaa cca cct gca aca aca aaa cca cct gca       1104
Thr Pro Pro Ala Thr Thr Lys Pro Pro Ala Thr Thr Lys Pro Pro Ala           
        355                 360                 365                       

aca aca ata ccg ccg tca gat gat ccg aat gca ata aag att aag gtg       1152
Thr Thr Ile Pro Pro Ser Asp Asp Pro Asn Ala Ile Lys Ile Lys Val           
    370                 375                 380                           

gac aca gta aat gca aaa ccg gga gac aca gta aat ata cct gta aga       1200
Asp Thr Val Asn Ala Lys Pro Gly Asp Thr Val Asn Ile Pro Val Arg           
385                 390                 395                 400           

ttc agt ggt ata cca tcc aag gga ata gca aac tgt gac ttt gta tac       1248
Phe Ser Gly Ile Pro Ser Lys Gly Ile Ala Asn Cys Asp Phe Val Tyr           
                405                 410                 415               

agc tat gac ccg aat gta ctt gag ata ata gag ata aaa ccg gga gaa       1296
Ser Tyr Asp Pro Asn Val Leu Glu Ile Ile Glu Ile Lys Pro Gly Glu           
            420                 425                 430                   

ttg ata gtt gac ccg aat cct gac aag agc ttt gat act gca gta tat       1344
Leu Ile Val Asp Pro Asn Pro Asp Lys Ser Phe Asp Thr Ala Val Tyr           
        435                 440                 445                       

cct gac aga aag ata ata gta ttc ctg ttt gca gaa gac agc gga aca       1392
Pro Asp Arg Lys Ile Ile Val Phe Leu Phe Ala Glu Asp Ser Gly Thr           
    450                 455                 460                           

gga gcg tat gca ata act aaa gac gga gta ttt gct acg ata gta gcg       1440
Gly Ala Tyr Ala Ile Thr Lys Asp Gly Val Phe Ala Thr Ile Val Ala           
465                 470                 475                 480           

aaa gta aaa tcc gga gca cct aac gga ctc agt gta atc aaa ttt gta       1488
Lys Val Lys Ser Gly Ala Pro Asn Gly Leu Ser Val Ile Lys Phe Val           
                485                 490                 495               

gaa gta ggc gga ttt gcg aac aat gac ctt gta gaa cag agg aca cag       1536
Glu Val Gly Gly Phe Ala Asn Asn Asp Leu Val Glu Gln Arg Thr Gln           
            500                 505                 510                   

ttc ttt gac ggt gga gta aat gtt gga gat ata gga tcc gcc ggt ggt       1584
Phe Phe Asp Gly Gly Val Asn Val Gly Asp Ile Gly Ser Ala Gly Gly           
        515                 520                 525                       

tta tcc gct gtg cag cct aat gtt agt tta ggc gaa gta ctg gat gtt       1632
Leu Ser Ala Val Gln Pro Asn Val Ser Leu Gly Glu Val Leu Asp Val           
    530                 535                 540                           

tct gct aac aga acc gct gct gac gga aca gtt gaa tgg ctt atc cca       1680
Ser Ala Asn Arg Thr Ala Ala Asp Gly Thr Val Glu Trp Leu Ile Pro           
545                 550                 555                 560           

aca gta act gca gct cca ggc cag acg gtc act atg ccc gta gta gtc       1728
Thr Val Thr Ala Ala Pro Gly Gln Thr Val Thr Met Pro Val Val Val           
                565                 570                 575               

aag agt tca agt ctt gca gtt gct ggt gcg cag ttc aag atc cag gcg       1776
Lys Ser Ser Ser Leu Ala Val Ala Gly Ala Gln Phe Lys Ile Gln Ala           
            580                 585                 590                   

gcg aca ggc gta agt tat tcg tcc aag acg gac ggt gac gct tac ggt       1824
Ala Thr Gly Val Ser Tyr Ser Ser Lys Thr Asp Gly Asp Ala Tyr Gly           
        595                 600                 605                       

tca ggc att gtg tac aat aat agt aag tat gct ttt gga cag ggt gca       1872
Ser Gly Ile Val Tyr Asn Asn Ser Lys Tyr Ala Phe Gly Gln Gly Ala           
    610                 615                 620                           

ggt aga gga ata gtt gca gct gat gat tcg gtt gtg ctt act ctt gca       1920
Gly Arg Gly Ile Val Ala Ala Asp Asp Ser Val Val Leu Thr Leu Ala           
625                 630                 635                 640           

tat aca gtt ccc gct gat tgt gct gaa ggt aca tat gat gtc aag tgg       1968
Tyr Thr Val Pro Ala Asp Cys Ala Glu Gly Thr Tyr Asp Val Lys Trp           
                645                 650                 655               

tct gat gcg ttt gta agt gat aca gac gga cag aat atc aca agt aag       2016
Ser Asp Ala Phe Val Ser Asp Thr Asp Gly Gln Asn Ile Thr Ser Lys           
            660                 665                 670                   

gtt act ctt act gat ggc gct atc att gtt aag tag                       2052
Val Thr Leu Thr Asp Gly Ala Ile Ile Val Lys                               
        675                 680                                           


<210>  2
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Gly Asp Ser Leu Lys Val Thr Val Gly Thr Ala Asn Gly Lys Pro 
1               5                   10                  15      


Gly Asp Thr Val Thr Val Pro Val Thr Phe Ala Asp Val Ala Lys Met 
            20                  25                  30          


Lys Asn Val Gly Thr Cys Asn Phe Tyr Leu Gly Tyr Asp Ala Ser Leu 
        35                  40                  45              


Leu Glu Val Val Ser Val Asp Ala Gly Pro Ile Val Lys Asn Ala Ala 
    50                  55                  60                  


Val Asn Phe Ser Ser Ser Ala Ser Asn Gly Thr Ile Ser Phe Leu Phe 
65                  70                  75                  80  


Leu Asp Asn Thr Ile Thr Asp Glu Leu Ile Thr Ala Asp Gly Val Phe 
                85                  90                  95      


Ala Asn Ile Lys Phe Lys Leu Lys Ser Val Thr Ala Lys Thr Thr Thr 
            100                 105                 110         


Pro Val Thr Phe Lys Asp Gly Gly Ala Phe Gly Asp Gly Thr Met Ser 
        115                 120                 125             


Lys Ile Ala Ser Val Thr Lys Thr Asn Gly Ser Val Thr Ile Asp Pro 
    130                 135                 140                 


Thr Lys Gly Ala Thr Pro Thr Asn Thr Ala Thr Pro Thr Lys Ser Ala 
145                 150                 155                 160 


Thr Ala Thr Pro Thr Arg Pro Ser Val Pro Thr Asn Thr Pro Thr Asn 
                165                 170                 175     


Thr Pro Ala Asn Thr Pro Val Ser Gly Asn Leu Lys Val Glu Phe Tyr 
            180                 185                 190         


Asn Ser Asn Pro Ser Asp Thr Thr Asn Ser Ile Asn Pro Gln Phe Lys 
        195                 200                 205             


Val Thr Asn Thr Gly Ser Ser Ala Ile Asp Leu Ser Lys Leu Thr Leu 
    210                 215                 220                 


Arg Tyr Tyr Tyr Thr Val Asp Gly Gln Lys Asp Gln Thr Phe Trp Cys 
225                 230                 235                 240 


Asp His Ala Ala Ile Ile Gly Ser Asn Gly Ser Tyr Asn Gly Ile Thr 
                245                 250                 255     


Ser Asn Val Lys Gly Thr Phe Val Lys Met Ser Ser Ser Thr Asn Asn 
            260                 265                 270         


Ala Asp Thr Tyr Leu Glu Ile Ser Phe Thr Gly Gly Thr Leu Glu Pro 
        275                 280                 285             


Gly Ala His Val Gln Ile Gln Gly Arg Phe Ala Lys Asn Asp Trp Ser 
    290                 295                 300                 


Asn Tyr Thr Gln Ser Asn Asp Tyr Ser Phe Lys Ser Ala Ser Gln Phe 
305                 310                 315                 320 


Val Glu Trp Asp Gln Val Thr Ala Tyr Leu Asn Gly Val Leu Val Trp 
                325                 330                 335     


Gly Lys Glu Pro Gly Gly Ser Val Val Pro Ser Thr Gln Pro Val Thr 
            340                 345                 350         


Thr Pro Pro Ala Thr Thr Lys Pro Pro Ala Thr Thr Lys Pro Pro Ala 
        355                 360                 365             


Thr Thr Ile Pro Pro Ser Asp Asp Pro Asn Ala Ile Lys Ile Lys Val 
    370                 375                 380                 


Asp Thr Val Asn Ala Lys Pro Gly Asp Thr Val Asn Ile Pro Val Arg 
385                 390                 395                 400 


Phe Ser Gly Ile Pro Ser Lys Gly Ile Ala Asn Cys Asp Phe Val Tyr 
                405                 410                 415     


Ser Tyr Asp Pro Asn Val Leu Glu Ile Ile Glu Ile Lys Pro Gly Glu 
            420                 425                 430         


Leu Ile Val Asp Pro Asn Pro Asp Lys Ser Phe Asp Thr Ala Val Tyr 
        435                 440                 445             


Pro Asp Arg Lys Ile Ile Val Phe Leu Phe Ala Glu Asp Ser Gly Thr 
    450                 455                 460                 


Gly Ala Tyr Ala Ile Thr Lys Asp Gly Val Phe Ala Thr Ile Val Ala 
465                 470                 475                 480 


Lys Val Lys Ser Gly Ala Pro Asn Gly Leu Ser Val Ile Lys Phe Val 
                485                 490                 495     


Glu Val Gly Gly Phe Ala Asn Asn Asp Leu Val Glu Gln Arg Thr Gln 
            500                 505                 510         


Phe Phe Asp Gly Gly Val Asn Val Gly Asp Ile Gly Ser Ala Gly Gly 
        515                 520                 525             


Leu Ser Ala Val Gln Pro Asn Val Ser Leu Gly Glu Val Leu Asp Val 
    530                 535                 540                 


Ser Ala Asn Arg Thr Ala Ala Asp Gly Thr Val Glu Trp Leu Ile Pro 
545                 550                 555                 560 


Thr Val Thr Ala Ala Pro Gly Gln Thr Val Thr Met Pro Val Val Val 
                565                 570                 575     


Lys Ser Ser Ser Leu Ala Val Ala Gly Ala Gln Phe Lys Ile Gln Ala 
            580                 585                 590         


Ala Thr Gly Val Ser Tyr Ser Ser Lys Thr Asp Gly Asp Ala Tyr Gly 
        595                 600                 605             


Ser Gly Ile Val Tyr Asn Asn Ser Lys Tyr Ala Phe Gly Gln Gly Ala 
    610                 615                 620                 


Gly Arg Gly Ile Val Ala Ala Asp Asp Ser Val Val Leu Thr Leu Ala 
625                 630                 635                 640 


Tyr Thr Val Pro Ala Asp Cys Ala Glu Gly Thr Tyr Asp Val Lys Trp 
                645                 650                 655     


Ser Asp Ala Phe Val Ser Asp Thr Asp Gly Gln Asn Ile Thr Ser Lys 
            660                 665                 670         


Val Thr Leu Thr Asp Gly Ala Ile Ile Val Lys 
        675                 680             


<210>  3
<211>  2580
<212>  DNA
<213>  C. cellulolytica


<220>
<221>  CDS
<222>  (1)..(2580)

<400>  3
atg ctt gtt ggg gca gga gat ttg att cga aac cat acc ttt gac aac         48
Met Leu Val Gly Ala Gly Asp Leu Ile Arg Asn His Thr Phe Asp Asn           
1               5                   10                  15                

aga gta ggt ctt cca tgg cac gtg gtt gaa tca tac cct gca aag gca         96
Arg Val Gly Leu Pro Trp His Val Val Glu Ser Tyr Pro Ala Lys Ala           
            20                  25                  30                    

agt ttt gaa att aca tct gat ggt aag tac aag ata act gct caa aag        144
Ser Phe Glu Ile Thr Ser Asp Gly Lys Tyr Lys Ile Thr Ala Gln Lys           
        35                  40                  45                        

atc ggt gag gca gga aaa ggt gaa aga tgg gat ata caa ttc cgt cac        192
Ile Gly Glu Ala Gly Lys Gly Glu Arg Trp Asp Ile Gln Phe Arg His           
    50                  55                  60                            

aga gga ctc gca ttg caa caa ggt cat act tat aca gta aag ttt act        240
Arg Gly Leu Ala Leu Gln Gln Gly His Thr Tyr Thr Val Lys Phe Thr           
65                  70                  75                  80            

gtt act gct agc aga gct tgt aaa att tat cct aaa ata ggt gac cag        288
Val Thr Ala Ser Arg Ala Cys Lys Ile Tyr Pro Lys Ile Gly Asp Gln           
                85                  90                  95                

ggt gat cca tat gat gaa tac tgg aat atg aat caa caa tgg aat ttc        336
Gly Asp Pro Tyr Asp Glu Tyr Trp Asn Met Asn Gln Gln Trp Asn Phe           
            100                 105                 110                   

ctg gaa tta cag gct aat act cca aaa act gta act cag aca ttt aca        384
Leu Glu Leu Gln Ala Asn Thr Pro Lys Thr Val Thr Gln Thr Phe Thr           
        115                 120                 125                       

cag act aag gga gat aag aag aac gtt gaa ttt gct ttt cac ctt gct        432
Gln Thr Lys Gly Asp Lys Lys Asn Val Glu Phe Ala Phe His Leu Ala           
    130                 135                 140                           

ccc gat aaa act aca tct gag gca cag aat cca gca agt ttc caa cct        480
Pro Asp Lys Thr Thr Ser Glu Ala Gln Asn Pro Ala Ser Phe Gln Pro           
145                 150                 155                 160           

ata aca tat act ttt gat gaa att tat att cag gac cct caa ttt gca        528
Ile Thr Tyr Thr Phe Asp Glu Ile Tyr Ile Gln Asp Pro Gln Phe Ala           
                165                 170                 175               

gga tat act gaa gat cca cct gaa cct act aat gtt gta cgt ttg aat        576
Gly Tyr Thr Glu Asp Pro Pro Glu Pro Thr Asn Val Val Arg Leu Asn           
            180                 185                 190                   

cag gta ggt ttc tat cct aat gct gat aag att gca aca gta gca aca        624
Gln Val Gly Phe Tyr Pro Asn Ala Asp Lys Ile Ala Thr Val Ala Thr           
        195                 200                 205                       

agt tca aca act cca att aac tgg cag ttg gtt aat agt act gga gca        672
Ser Ser Thr Thr Pro Ile Asn Trp Gln Leu Val Asn Ser Thr Gly Ala           
    210                 215                 220                           

gct gtt tta aca ggt aaa tca act gtt aaa ggt gcc gac cgt gca tca        720
Ala Val Leu Thr Gly Lys Ser Thr Val Lys Gly Ala Asp Arg Ala Ser           
225                 230                 235                 240           

ggt gat aat gtc cat atc att gat ttc tct agt tac aca aca cct ggt        768
Gly Asp Asn Val His Ile Ile Asp Phe Ser Ser Tyr Thr Thr Pro Gly           
                245                 250                 255               

acc gac tat aag ata gta aca gat gta tca gta aca aaa gcc gga gac        816
Thr Asp Tyr Lys Ile Val Thr Asp Val Ser Val Thr Lys Ala Gly Asp           
            260                 265                 270                   

aat gaa agt atg aag ttc aat att gga gat gac ctt ttt act caa atg        864
Asn Glu Ser Met Lys Phe Asn Ile Gly Asp Asp Leu Phe Thr Gln Met           
        275                 280                 285                       

aaa tac gat tca atg aag tat ttc tat cac aac aga agt gct att cca        912
Lys Tyr Asp Ser Met Lys Tyr Phe Tyr His Asn Arg Ser Ala Ile Pro           
    290                 295                 300                           

ata caa atg cca tac tgt gat caa tca caa tgg gca cgt cct gca gga        960
Ile Gln Met Pro Tyr Cys Asp Gln Ser Gln Trp Ala Arg Pro Ala Gly           
305                 310                 315                 320           

cac aca act gat ata ctt gct cca gat cca aca aag gat tac aag gct       1008
His Thr Thr Asp Ile Leu Ala Pro Asp Pro Thr Lys Asp Tyr Lys Ala           
                325                 330                 335               

aac tac aca ctt gac gtt aca ggt ggt tgg tat gat gcc ggt gac cat       1056
Asn Tyr Thr Leu Asp Val Thr Gly Gly Trp Tyr Asp Ala Gly Asp His           
            340                 345                 350                   

ggt aag tat gtt gtt aat ggt ggt att gca acc tgg acc gta atg aat       1104
Gly Lys Tyr Val Val Asn Gly Gly Ile Ala Thr Trp Thr Val Met Asn           
        355                 360                 365                       

gca tat gag cgt gca cta cac atg ggt gga gac act tca gtt gct cca       1152
Ala Tyr Glu Arg Ala Leu His Met Gly Gly Asp Thr Ser Val Ala Pro           
    370                 375                 380                           

ttt aaa gac ggt tct tta gca ata cca gaa gcg gaa gtc tat cct gac       1200
Phe Lys Asp Gly Ser Leu Ala Ile Pro Glu Ala Glu Val Tyr Pro Asp           
385                 390                 395                 400           

ata ctg gac gaa gct cgt tac cag ctc att aac atg aaa aca tta tta       1248
Ile Leu Asp Glu Ala Arg Tyr Gln Leu Ile Asn Met Lys Thr Leu Leu           
                405                 410                 415               

aat agt cag gtt cca gca gga aag tat gcg ggt atg gct cac cac aaa       1296
Asn Ser Gln Val Pro Ala Gly Lys Tyr Ala Gly Met Ala His His Lys           
            420                 425                 430                   

gct cat gac gaa cgt tgg aca gct ctt gct gta cgt ccc gac cag gat       1344
Ala His Asp Glu Arg Trp Thr Ala Leu Ala Val Arg Pro Asp Gln Asp           
        435                 440                 445                       

aca atg aaa cgt tgg ttg cag cct cca agt aca gca gct aca tta aat       1392
Thr Met Lys Arg Trp Leu Gln Pro Pro Ser Thr Ala Ala Thr Leu Asn           
    450                 455                 460                           

ctg gct gct att gct gca caa agt tca cgt ctt tgg aaa cag ttt gat       1440
Leu Ala Ala Ile Ala Ala Gln Ser Ser Arg Leu Trp Lys Gln Phe Asp           
465                 470                 475                 480           

tct gct ttc gca act aag tgt tta act gca gca gaa act gct agg gat       1488
Ser Ala Phe Ala Thr Lys Cys Leu Thr Ala Ala Glu Thr Ala Arg Asp           
                485                 490                 495               

gca gct gta gct cat cca gaa ata tat gca act atg gaa cag ggt gcc       1536
Ala Ala Val Ala His Pro Glu Ile Tyr Ala Thr Met Glu Gln Gly Ala           
            500                 505                 510                   

ggt ggt gga gca tac gga gac aac tat gtt ctt gat gat ttc tac tgg       1584
Gly Gly Gly Ala Tyr Gly Asp Asn Tyr Val Leu Asp Asp Phe Tyr Trp           
        515                 520                 525                       

gca gca tgt gaa ttg tat gca act aca ggc agt gac aag tat ttg aac       1632
Ala Ala Cys Glu Leu Tyr Ala Thr Thr Gly Ser Asp Lys Tyr Leu Asn           
    530                 535                 540                           

tac ata aag agc tca aag cat tat ctc gaa atg cct aca gaa tta aca       1680
Tyr Ile Lys Ser Ser Lys His Tyr Leu Glu Met Pro Thr Glu Leu Thr           
545                 550                 555                 560           

ggc ggt gag aat act gga att aca ggg gct ttt gac tgg ggt tgt aca       1728
Gly Gly Glu Asn Thr Gly Ile Thr Gly Ala Phe Asp Trp Gly Cys Thr           
                565                 570                 575               

gca ggt atg gga aca ata aca ctt gca ctt gta cct aca aag ctt ccg       1776
Ala Gly Met Gly Thr Ile Thr Leu Ala Leu Val Pro Thr Lys Leu Pro           
            580                 585                 590                   

gca gca gat gtt gct aca gct aaa gct aat att caa gct gca gct gat       1824
Ala Ala Asp Val Ala Thr Ala Lys Ala Asn Ile Gln Ala Ala Ala Asp           
        595                 600                 605                       

aag ttc ata tca att tca aaa gca caa ggc tat ggt gta cca cta gaa       1872
Lys Phe Ile Ser Ile Ser Lys Ala Gln Gly Tyr Gly Val Pro Leu Glu           
    610                 615                 620                           

gaa aaa gta att tca tct cct ttt gat gca tct gtt gtt aaa ggt ttc       1920
Glu Lys Val Ile Ser Ser Pro Phe Asp Ala Ser Val Val Lys Gly Phe           
625                 630                 635                 640           

caa tgg gga tca aac tca ttc gtt att aat gaa gca ata gtt atg tca       1968
Gln Trp Gly Ser Asn Ser Phe Val Ile Asn Glu Ala Ile Val Met Ser           
                645                 650                 655               

tat gct tat gaa ttc agc gat gtt aat ggc aca aag aat aat aaa tat       2016
Tyr Ala Tyr Glu Phe Ser Asp Val Asn Gly Thr Lys Asn Asn Lys Tyr           
            660                 665                 670                   

att aat ggt gct tta aca gca atg gat tac ctc ctc gga cgt aac cca       2064
Ile Asn Gly Ala Leu Thr Ala Met Asp Tyr Leu Leu Gly Arg Asn Pro           
        675                 680                 685                       

aat att caa agc tat ata act ggt tat ggt gac aac cca ctt gaa aat       2112
Asn Ile Gln Ser Tyr Ile Thr Gly Tyr Gly Asp Asn Pro Leu Glu Asn           
    690                 695                 700                           

cct cat cac cgt ttc tgg gca tac cag gca gac aac aca ttc cca aaa       2160
Pro His His Arg Phe Trp Ala Tyr Gln Ala Asp Asn Thr Phe Pro Lys           
705                 710                 715                 720           

cca cct ccg gga tgt ctg tca gga gga cct aac tcc ggc ttg cag gat       2208
Pro Pro Pro Gly Cys Leu Ser Gly Gly Pro Asn Ser Gly Leu Gln Asp           
                725                 730                 735               

cct tgg gtt aag ggt tca ggc tgg cag cca ggt gaa aga cct gct gaa       2256
Pro Trp Val Lys Gly Ser Gly Trp Gln Pro Gly Glu Arg Pro Ala Glu           
            740                 745                 750                   

aaa tgc ttc atg gac aat att gaa tct tgg tca aca aac gaa ata acc       2304
Lys Cys Phe Met Asp Asn Ile Glu Ser Trp Ser Thr Asn Glu Ile Thr           
        755                 760                 765                       

atc aac tgg aat gct cct ctt gta tgg ata tca gct tac ctt gat gaa       2352
Ile Asn Trp Asn Ala Pro Leu Val Trp Ile Ser Ala Tyr Leu Asp Glu           
    770                 775                 780                           

aag ggg cca gag att ggt ggg tca gtg act cct cca act aat tta gga       2400
Lys Gly Pro Glu Ile Gly Gly Ser Val Thr Pro Pro Thr Asn Leu Gly           
785                 790                 795                 800           

gat gtt aac ggc gat gga aac aag gat gca ttg gac ttc gct gca ttg       2448
Asp Val Asn Gly Asp Gly Asn Lys Asp Ala Leu Asp Phe Ala Ala Leu           
                805                 810                 815               

aag aaa gcc ttg tta agc cag gat act tct act ata aat gtt gct aat       2496
Lys Lys Ala Leu Leu Ser Gln Asp Thr Ser Thr Ile Asn Val Ala Asn           
            820                 825                 830                   

gct gat ata aac aaa gat ggt tct att gat gca gtt gac ttt gca tta       2544
Ala Asp Ile Asn Lys Asp Gly Ser Ile Asp Ala Val Asp Phe Ala Leu           
        835                 840                 845                       

ctc aaa tca ttc ttg tta gga aaa atc aca cag tga                       2580
Leu Lys Ser Phe Leu Leu Gly Lys Ile Thr Gln                               
    850                 855                                               


<210>  4
<211>  859
<212>  PRT
<213>  C. cellulolytica

<400>  4

Met Leu Val Gly Ala Gly Asp Leu Ile Arg Asn His Thr Phe Asp Asn 
1               5                   10                  15      


Arg Val Gly Leu Pro Trp His Val Val Glu Ser Tyr Pro Ala Lys Ala 
            20                  25                  30          


Ser Phe Glu Ile Thr Ser Asp Gly Lys Tyr Lys Ile Thr Ala Gln Lys 
        35                  40                  45              


Ile Gly Glu Ala Gly Lys Gly Glu Arg Trp Asp Ile Gln Phe Arg His 
    50                  55                  60                  


Arg Gly Leu Ala Leu Gln Gln Gly His Thr Tyr Thr Val Lys Phe Thr 
65                  70                  75                  80  


Val Thr Ala Ser Arg Ala Cys Lys Ile Tyr Pro Lys Ile Gly Asp Gln 
                85                  90                  95      


Gly Asp Pro Tyr Asp Glu Tyr Trp Asn Met Asn Gln Gln Trp Asn Phe 
            100                 105                 110         


Leu Glu Leu Gln Ala Asn Thr Pro Lys Thr Val Thr Gln Thr Phe Thr 
        115                 120                 125             


Gln Thr Lys Gly Asp Lys Lys Asn Val Glu Phe Ala Phe His Leu Ala 
    130                 135                 140                 


Pro Asp Lys Thr Thr Ser Glu Ala Gln Asn Pro Ala Ser Phe Gln Pro 
145                 150                 155                 160 


Ile Thr Tyr Thr Phe Asp Glu Ile Tyr Ile Gln Asp Pro Gln Phe Ala 
                165                 170                 175     


Gly Tyr Thr Glu Asp Pro Pro Glu Pro Thr Asn Val Val Arg Leu Asn 
            180                 185                 190         


Gln Val Gly Phe Tyr Pro Asn Ala Asp Lys Ile Ala Thr Val Ala Thr 
        195                 200                 205             


Ser Ser Thr Thr Pro Ile Asn Trp Gln Leu Val Asn Ser Thr Gly Ala 
    210                 215                 220                 


Ala Val Leu Thr Gly Lys Ser Thr Val Lys Gly Ala Asp Arg Ala Ser 
225                 230                 235                 240 


Gly Asp Asn Val His Ile Ile Asp Phe Ser Ser Tyr Thr Thr Pro Gly 
                245                 250                 255     


Thr Asp Tyr Lys Ile Val Thr Asp Val Ser Val Thr Lys Ala Gly Asp 
            260                 265                 270         


Asn Glu Ser Met Lys Phe Asn Ile Gly Asp Asp Leu Phe Thr Gln Met 
        275                 280                 285             


Lys Tyr Asp Ser Met Lys Tyr Phe Tyr His Asn Arg Ser Ala Ile Pro 
    290                 295                 300                 


Ile Gln Met Pro Tyr Cys Asp Gln Ser Gln Trp Ala Arg Pro Ala Gly 
305                 310                 315                 320 


His Thr Thr Asp Ile Leu Ala Pro Asp Pro Thr Lys Asp Tyr Lys Ala 
                325                 330                 335     


Asn Tyr Thr Leu Asp Val Thr Gly Gly Trp Tyr Asp Ala Gly Asp His 
            340                 345                 350         


Gly Lys Tyr Val Val Asn Gly Gly Ile Ala Thr Trp Thr Val Met Asn 
        355                 360                 365             


Ala Tyr Glu Arg Ala Leu His Met Gly Gly Asp Thr Ser Val Ala Pro 
    370                 375                 380                 


Phe Lys Asp Gly Ser Leu Ala Ile Pro Glu Ala Glu Val Tyr Pro Asp 
385                 390                 395                 400 


Ile Leu Asp Glu Ala Arg Tyr Gln Leu Ile Asn Met Lys Thr Leu Leu 
                405                 410                 415     


Asn Ser Gln Val Pro Ala Gly Lys Tyr Ala Gly Met Ala His His Lys 
            420                 425                 430         


Ala His Asp Glu Arg Trp Thr Ala Leu Ala Val Arg Pro Asp Gln Asp 
        435                 440                 445             


Thr Met Lys Arg Trp Leu Gln Pro Pro Ser Thr Ala Ala Thr Leu Asn 
    450                 455                 460                 


Leu Ala Ala Ile Ala Ala Gln Ser Ser Arg Leu Trp Lys Gln Phe Asp 
465                 470                 475                 480 


Ser Ala Phe Ala Thr Lys Cys Leu Thr Ala Ala Glu Thr Ala Arg Asp 
                485                 490                 495     


Ala Ala Val Ala His Pro Glu Ile Tyr Ala Thr Met Glu Gln Gly Ala 
            500                 505                 510         


Gly Gly Gly Ala Tyr Gly Asp Asn Tyr Val Leu Asp Asp Phe Tyr Trp 
        515                 520                 525             


Ala Ala Cys Glu Leu Tyr Ala Thr Thr Gly Ser Asp Lys Tyr Leu Asn 
    530                 535                 540                 


Tyr Ile Lys Ser Ser Lys His Tyr Leu Glu Met Pro Thr Glu Leu Thr 
545                 550                 555                 560 


Gly Gly Glu Asn Thr Gly Ile Thr Gly Ala Phe Asp Trp Gly Cys Thr 
                565                 570                 575     


Ala Gly Met Gly Thr Ile Thr Leu Ala Leu Val Pro Thr Lys Leu Pro 
            580                 585                 590         


Ala Ala Asp Val Ala Thr Ala Lys Ala Asn Ile Gln Ala Ala Ala Asp 
        595                 600                 605             


Lys Phe Ile Ser Ile Ser Lys Ala Gln Gly Tyr Gly Val Pro Leu Glu 
    610                 615                 620                 


Glu Lys Val Ile Ser Ser Pro Phe Asp Ala Ser Val Val Lys Gly Phe 
625                 630                 635                 640 


Gln Trp Gly Ser Asn Ser Phe Val Ile Asn Glu Ala Ile Val Met Ser 
                645                 650                 655     


Tyr Ala Tyr Glu Phe Ser Asp Val Asn Gly Thr Lys Asn Asn Lys Tyr 
            660                 665                 670         


Ile Asn Gly Ala Leu Thr Ala Met Asp Tyr Leu Leu Gly Arg Asn Pro 
        675                 680                 685             


Asn Ile Gln Ser Tyr Ile Thr Gly Tyr Gly Asp Asn Pro Leu Glu Asn 
    690                 695                 700                 


Pro His His Arg Phe Trp Ala Tyr Gln Ala Asp Asn Thr Phe Pro Lys 
705                 710                 715                 720 


Pro Pro Pro Gly Cys Leu Ser Gly Gly Pro Asn Ser Gly Leu Gln Asp 
                725                 730                 735     


Pro Trp Val Lys Gly Ser Gly Trp Gln Pro Gly Glu Arg Pro Ala Glu 
            740                 745                 750         


Lys Cys Phe Met Asp Asn Ile Glu Ser Trp Ser Thr Asn Glu Ile Thr 
        755                 760                 765             


Ile Asn Trp Asn Ala Pro Leu Val Trp Ile Ser Ala Tyr Leu Asp Glu 
    770                 775                 780                 


Lys Gly Pro Glu Ile Gly Gly Ser Val Thr Pro Pro Thr Asn Leu Gly 
785                 790                 795                 800 


Asp Val Asn Gly Asp Gly Asn Lys Asp Ala Leu Asp Phe Ala Ala Leu 
                805                 810                 815     


Lys Lys Ala Leu Leu Ser Gln Asp Thr Ser Thr Ile Asn Val Ala Asn 
            820                 825                 830         


Ala Asp Ile Asn Lys Asp Gly Ser Ile Asp Ala Val Asp Phe Ala Leu 
        835                 840                 845             


Leu Lys Ser Phe Leu Leu Gly Lys Ile Thr Gln 
    850                 855                 


<210>  5
<211>  2118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion consruct enzyme-dockerin


<220>
<221>  CDS
<222>  (1)..(2118)

<400>  5
atg gga aca tat aac tat gga gaa gca tta cag aaa tca ata atg ttc         48
Met Gly Thr Tyr Asn Tyr Gly Glu Ala Leu Gln Lys Ser Ile Met Phe           
1               5                   10                  15                

tat gaa ttc cag cgt tcg gga gat ctt ccg gct gat aaa cgt gac aac         96
Tyr Glu Phe Gln Arg Ser Gly Asp Leu Pro Ala Asp Lys Arg Asp Asn           
            20                  25                  30                    

tgg aga gac gat tcc ggt atg aaa gac ggt tct gat gta gga gtt gat        144
Trp Arg Asp Asp Ser Gly Met Lys Asp Gly Ser Asp Val Gly Val Asp           
        35                  40                  45                        

ctt aca gga gga tgg tac gat gca ggt gac cat gtg aaa ttt aat cta        192
Leu Thr Gly Gly Trp Tyr Asp Ala Gly Asp His Val Lys Phe Asn Leu           
    50                  55                  60                            

cct atg tca tat aca tct gca atg ctt gca tgg tcc tta tat gag gat        240
Pro Met Ser Tyr Thr Ser Ala Met Leu Ala Trp Ser Leu Tyr Glu Asp           
65                  70                  75                  80            

aag gat gct tat gat aag agc ggt cag aca aaa tat ata atg gac ggt        288
Lys Asp Ala Tyr Asp Lys Ser Gly Gln Thr Lys Tyr Ile Met Asp Gly           
                85                  90                  95                

ata aaa tgg gct aat gat tat ttt att aaa tgt aat ccg aca ccc ggt        336
Ile Lys Trp Ala Asn Asp Tyr Phe Ile Lys Cys Asn Pro Thr Pro Gly           
            100                 105                 110                   

gta tat tat tac caa gta gga gac ggc gga aag gac cac tct tgg tgg        384
Val Tyr Tyr Tyr Gln Val Gly Asp Gly Gly Lys Asp His Ser Trp Trp           
        115                 120                 125                       

ggc cct gcg gaa gta atg cag atg gaa aga ccg tct ttt aag gtt gac        432
Gly Pro Ala Glu Val Met Gln Met Glu Arg Pro Ser Phe Lys Val Asp           
    130                 135                 140                           

gct tct aag ccc ggt tct gca gta tgt gct tcc act gca gct tct ctg        480
Ala Ser Lys Pro Gly Ser Ala Val Cys Ala Ser Thr Ala Ala Ser Leu           
145                 150                 155                 160           

gca tct gca gca gta gtc ttt aaa tcc agt gat cct act tat gca gaa        528
Ala Ser Ala Ala Val Val Phe Lys Ser Ser Asp Pro Thr Tyr Ala Glu           
                165                 170                 175               

aag tgc ata agc cat gca aag aac ctg ttt gat atg gct gac aaa gca        576
Lys Cys Ile Ser His Ala Lys Asn Leu Phe Asp Met Ala Asp Lys Ala           
            180                 185                 190                   

aag agt gat gct ggt tat act gcg gct tca ggc tac tac agc tca agc        624
Lys Ser Asp Ala Gly Tyr Thr Ala Ala Ser Gly Tyr Tyr Ser Ser Ser           
        195                 200                 205                       

tca ttt tac gat gat ctc tca tgg gct gca gta tgg tta tat ctt gct        672
Ser Phe Tyr Asp Asp Leu Ser Trp Ala Ala Val Trp Leu Tyr Leu Ala           
    210                 215                 220                           

aca aat gac agt aca tat tta gac aaa gca gaa tcc tat gta ccg aat        720
Thr Asn Asp Ser Thr Tyr Leu Asp Lys Ala Glu Ser Tyr Val Pro Asn           
225                 230                 235                 240           

tgg ggt aaa gaa cag cag aca gat att atc gcc tac aag tgg gga cag        768
Trp Gly Lys Glu Gln Gln Thr Asp Ile Ile Ala Tyr Lys Trp Gly Gln           
                245                 250                 255               

tgc tgg gat gat gtt cat tat ggt gct gag ctt ctt ctt gca aag ctt        816
Cys Trp Asp Asp Val His Tyr Gly Ala Glu Leu Leu Leu Ala Lys Leu           
            260                 265                 270                   

aca aac aaa caa ttg tat aag gat agt ata gaa atg aac ctt gac ttc        864
Thr Asn Lys Gln Leu Tyr Lys Asp Ser Ile Glu Met Asn Leu Asp Phe           
        275                 280                 285                       

tgg aca act ggt gtt aac gga aca cgt gtt tct tac acg cca aag ggt        912
Trp Thr Thr Gly Val Asn Gly Thr Arg Val Ser Tyr Thr Pro Lys Gly           
    290                 295                 300                           

ttg gcg tgg cta ttc caa tgg ggt tca tta aga cat gct aca act cag        960
Leu Ala Trp Leu Phe Gln Trp Gly Ser Leu Arg His Ala Thr Thr Gln           
305                 310                 315                 320           

gct ttt tta gcc ggt gtt tat gca gag tgg gaa ggc tgt acg cca tcc       1008
Ala Phe Leu Ala Gly Val Tyr Ala Glu Trp Glu Gly Cys Thr Pro Ser           
                325                 330                 335               

aaa gta tct gta tat aag gat ttc ctc aag agt caa att gat tat gca       1056
Lys Val Ser Val Tyr Lys Asp Phe Leu Lys Ser Gln Ile Asp Tyr Ala           
            340                 345                 350                   

ctt ggc agt acc gga aga agt ttt gtt gtc gga tat gga gta aat cct       1104
Leu Gly Ser Thr Gly Arg Ser Phe Val Val Gly Tyr Gly Val Asn Pro           
        355                 360                 365                       

cct caa cat cct cat cac aga act gct cac ggt tca tgg aca gat caa       1152
Pro Gln His Pro His His Arg Thr Ala His Gly Ser Trp Thr Asp Gln           
    370                 375                 380                           

atg act tca cca aca tac cac agg cat act att tat ggt gcg ttg gta       1200
Met Thr Ser Pro Thr Tyr His Arg His Thr Ile Tyr Gly Ala Leu Val           
385                 390                 395                 400           

gga gga ccg gat aat gca gat ggc tat act gat gaa ata aac aat tat       1248
Gly Gly Pro Asp Asn Ala Asp Gly Tyr Thr Asp Glu Ile Asn Asn Tyr           
                405                 410                 415               

gtc aat aat gaa ata gcc tgc gat tat aat gcc gga ttt aca ggt gca       1296
Val Asn Asn Glu Ile Ala Cys Asp Tyr Asn Ala Gly Phe Thr Gly Ala           
            420                 425                 430                   

ctt gca aaa atg tac aag cat tct ggc gga gat ccg att cca aac ttc       1344
Leu Ala Lys Met Tyr Lys His Ser Gly Gly Asp Pro Ile Pro Asn Phe           
        435                 440                 445                       

aag gct atc gaa aaa ata acc aac gat gaa gtt att ata aag gca ggt       1392
Lys Ala Ile Glu Lys Ile Thr Asn Asp Glu Val Ile Ile Lys Ala Gly           
    450                 455                 460                           

ttg aat tca act ggc cct aac tac act gaa atc aag gct gtt gtt tat       1440
Leu Asn Ser Thr Gly Pro Asn Tyr Thr Glu Ile Lys Ala Val Val Tyr           
465                 470                 475                 480           

aac cag aca gga tgg cct gca aga gtt acg gac aag ata tca ttt aaa       1488
Asn Gln Thr Gly Trp Pro Ala Arg Val Thr Asp Lys Ile Ser Phe Lys           
                485                 490                 495               

tat ttt atg gac ttg tct gaa att gta gca gca gga att gat cct tta       1536
Tyr Phe Met Asp Leu Ser Glu Ile Val Ala Ala Gly Ile Asp Pro Leu           
            500                 505                 510                   

agc ctt gta aca agt tca aat tat tct gaa ggt aag aat act aag gtt       1584
Ser Leu Val Thr Ser Ser Asn Tyr Ser Glu Gly Lys Asn Thr Lys Val           
        515                 520                 525                       

tcc ggt gtg ttg cca tgg gat gtt tca aat aat gtt tac tat gta aat       1632
Ser Gly Val Leu Pro Trp Asp Val Ser Asn Asn Val Tyr Tyr Val Asn           
    530                 535                 540                           

gtt gat ttg aca gga gaa aat atc tac cca ggc ggt cag tct gcg tgc       1680
Val Asp Leu Thr Gly Glu Asn Ile Tyr Pro Gly Gly Gln Ser Ala Cys           
545                 550                 555                 560           

aga cga gaa gtt cag ttc aga att gcc gca cca cag gga aga aga tat       1728
Arg Arg Glu Val Gln Phe Arg Ile Ala Ala Pro Gln Gly Arg Arg Tyr           
                565                 570                 575               

tgg aat ccg aaa aat gat ttc tca tat gat gga tta cca acc acc agt       1776
Trp Asn Pro Lys Asn Asp Phe Ser Tyr Asp Gly Leu Pro Thr Thr Ser           
            580                 585                 590                   

act gta aat acg gtt acc aac ata cct gtt tat gat aac ggc gta aaa       1824
Thr Val Asn Thr Val Thr Asn Ile Pro Val Tyr Asp Asn Gly Val Lys           
        595                 600                 605                       

gta ttt ggt aac gaa ccc gca ggt gga tca gaa ccc ggc aca aag ctc       1872
Val Phe Gly Asn Glu Pro Ala Gly Gly Ser Glu Pro Gly Thr Lys Leu           
    610                 615                 620                           

gtt cct aca tgg ggc gat aca aac tgc gac ggc gtt gta aat gtt gct       1920
Val Pro Thr Trp Gly Asp Thr Asn Cys Asp Gly Val Val Asn Val Ala           
625                 630                 635                 640           

gac gta gta gtt ctt aac aga ttc ctc aac gat cct aca tat tct aac       1968
Asp Val Val Val Leu Asn Arg Phe Leu Asn Asp Pro Thr Tyr Ser Asn           
                645                 650                 655               

att act gat cag ggt aag gtt aac gca gac gtt gtt gat cct cag gat       2016
Ile Thr Asp Gln Gly Lys Val Asn Ala Asp Val Val Asp Pro Gln Asp           
            660                 665                 670                   

aag tcc ggc gca gca gtt gat cct gca ggc gta aag ctc aca gta gct       2064
Lys Ser Gly Ala Ala Val Asp Pro Ala Gly Val Lys Leu Thr Val Ala           
        675                 680                 685                       

gac tct gag gca atc ctc aag gct atc gtt gaa ctc atc aca ctt cct       2112
Asp Ser Glu Ala Ile Leu Lys Ala Ile Val Glu Leu Ile Thr Leu Pro           
    690                 695                 700                           

caa tga                                                               2118
Gln                                                                       
705                                                                       


<210>  6
<211>  705
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Gly Thr Tyr Asn Tyr Gly Glu Ala Leu Gln Lys Ser Ile Met Phe 
1               5                   10                  15      


Tyr Glu Phe Gln Arg Ser Gly Asp Leu Pro Ala Asp Lys Arg Asp Asn 
            20                  25                  30          


Trp Arg Asp Asp Ser Gly Met Lys Asp Gly Ser Asp Val Gly Val Asp 
        35                  40                  45              


Leu Thr Gly Gly Trp Tyr Asp Ala Gly Asp His Val Lys Phe Asn Leu 
    50                  55                  60                  


Pro Met Ser Tyr Thr Ser Ala Met Leu Ala Trp Ser Leu Tyr Glu Asp 
65                  70                  75                  80  


Lys Asp Ala Tyr Asp Lys Ser Gly Gln Thr Lys Tyr Ile Met Asp Gly 
                85                  90                  95      


Ile Lys Trp Ala Asn Asp Tyr Phe Ile Lys Cys Asn Pro Thr Pro Gly 
            100                 105                 110         


Val Tyr Tyr Tyr Gln Val Gly Asp Gly Gly Lys Asp His Ser Trp Trp 
        115                 120                 125             


Gly Pro Ala Glu Val Met Gln Met Glu Arg Pro Ser Phe Lys Val Asp 
    130                 135                 140                 


Ala Ser Lys Pro Gly Ser Ala Val Cys Ala Ser Thr Ala Ala Ser Leu 
145                 150                 155                 160 


Ala Ser Ala Ala Val Val Phe Lys Ser Ser Asp Pro Thr Tyr Ala Glu 
                165                 170                 175     


Lys Cys Ile Ser His Ala Lys Asn Leu Phe Asp Met Ala Asp Lys Ala 
            180                 185                 190         


Lys Ser Asp Ala Gly Tyr Thr Ala Ala Ser Gly Tyr Tyr Ser Ser Ser 
        195                 200                 205             


Ser Phe Tyr Asp Asp Leu Ser Trp Ala Ala Val Trp Leu Tyr Leu Ala 
    210                 215                 220                 


Thr Asn Asp Ser Thr Tyr Leu Asp Lys Ala Glu Ser Tyr Val Pro Asn 
225                 230                 235                 240 


Trp Gly Lys Glu Gln Gln Thr Asp Ile Ile Ala Tyr Lys Trp Gly Gln 
                245                 250                 255     


Cys Trp Asp Asp Val His Tyr Gly Ala Glu Leu Leu Leu Ala Lys Leu 
            260                 265                 270         


Thr Asn Lys Gln Leu Tyr Lys Asp Ser Ile Glu Met Asn Leu Asp Phe 
        275                 280                 285             


Trp Thr Thr Gly Val Asn Gly Thr Arg Val Ser Tyr Thr Pro Lys Gly 
    290                 295                 300                 


Leu Ala Trp Leu Phe Gln Trp Gly Ser Leu Arg His Ala Thr Thr Gln 
305                 310                 315                 320 


Ala Phe Leu Ala Gly Val Tyr Ala Glu Trp Glu Gly Cys Thr Pro Ser 
                325                 330                 335     


Lys Val Ser Val Tyr Lys Asp Phe Leu Lys Ser Gln Ile Asp Tyr Ala 
            340                 345                 350         


Leu Gly Ser Thr Gly Arg Ser Phe Val Val Gly Tyr Gly Val Asn Pro 
        355                 360                 365             


Pro Gln His Pro His His Arg Thr Ala His Gly Ser Trp Thr Asp Gln 
    370                 375                 380                 


Met Thr Ser Pro Thr Tyr His Arg His Thr Ile Tyr Gly Ala Leu Val 
385                 390                 395                 400 


Gly Gly Pro Asp Asn Ala Asp Gly Tyr Thr Asp Glu Ile Asn Asn Tyr 
                405                 410                 415     


Val Asn Asn Glu Ile Ala Cys Asp Tyr Asn Ala Gly Phe Thr Gly Ala 
            420                 425                 430         


Leu Ala Lys Met Tyr Lys His Ser Gly Gly Asp Pro Ile Pro Asn Phe 
        435                 440                 445             


Lys Ala Ile Glu Lys Ile Thr Asn Asp Glu Val Ile Ile Lys Ala Gly 
    450                 455                 460                 


Leu Asn Ser Thr Gly Pro Asn Tyr Thr Glu Ile Lys Ala Val Val Tyr 
465                 470                 475                 480 


Asn Gln Thr Gly Trp Pro Ala Arg Val Thr Asp Lys Ile Ser Phe Lys 
                485                 490                 495     


Tyr Phe Met Asp Leu Ser Glu Ile Val Ala Ala Gly Ile Asp Pro Leu 
            500                 505                 510         


Ser Leu Val Thr Ser Ser Asn Tyr Ser Glu Gly Lys Asn Thr Lys Val 
        515                 520                 525             


Ser Gly Val Leu Pro Trp Asp Val Ser Asn Asn Val Tyr Tyr Val Asn 
    530                 535                 540                 


Val Asp Leu Thr Gly Glu Asn Ile Tyr Pro Gly Gly Gln Ser Ala Cys 
545                 550                 555                 560 


Arg Arg Glu Val Gln Phe Arg Ile Ala Ala Pro Gln Gly Arg Arg Tyr 
                565                 570                 575     


Trp Asn Pro Lys Asn Asp Phe Ser Tyr Asp Gly Leu Pro Thr Thr Ser 
            580                 585                 590         


Thr Val Asn Thr Val Thr Asn Ile Pro Val Tyr Asp Asn Gly Val Lys 
        595                 600                 605             


Val Phe Gly Asn Glu Pro Ala Gly Gly Ser Glu Pro Gly Thr Lys Leu 
    610                 615                 620                 


Val Pro Thr Trp Gly Asp Thr Asn Cys Asp Gly Val Val Asn Val Ala 
625                 630                 635                 640 


Asp Val Val Val Leu Asn Arg Phe Leu Asn Asp Pro Thr Tyr Ser Asn 
                645                 650                 655     


Ile Thr Asp Gln Gly Lys Val Asn Ala Asp Val Val Asp Pro Gln Asp 
            660                 665                 670         


Lys Ser Gly Ala Ala Val Asp Pro Ala Gly Val Lys Leu Thr Val Ala 
        675                 680                 685             


Asp Ser Glu Ala Ile Leu Lys Ala Ile Val Glu Leu Ile Thr Leu Pro 
    690                 695                 700                 


Gln 
705 


<210>  7
<211>  1341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion construct enzyme-dockerin


<220>
<221>  CDS
<222>  (1)..(1341)

<400>  7
atg gca ggt gtg cct ttt aac aca aaa tac ccc tat ggt cct act tct         48
Met Ala Gly Val Pro Phe Asn Thr Lys Tyr Pro Tyr Gly Pro Thr Ser           
1               5                   10                  15                

att gcc gat aat cag tcg gaa gta act gca atg ctc aaa gca gaa tgg         96
Ile Ala Asp Asn Gln Ser Glu Val Thr Ala Met Leu Lys Ala Glu Trp           
            20                  25                  30                    

gaa gac tgg aag agc aag aga att acc tcg aac ggt gca gga gga tac        144
Glu Asp Trp Lys Ser Lys Arg Ile Thr Ser Asn Gly Ala Gly Gly Tyr           
        35                  40                  45                        

aag aga gta cag cgt gat gct tcc acc aat tat gat acg gta tcc gaa        192
Lys Arg Val Gln Arg Asp Ala Ser Thr Asn Tyr Asp Thr Val Ser Glu           
    50                  55                  60                            

ggt atg gga tac gga ctt ctt ttg gcg gtt tgc ttt aac gaa cag gct        240
Gly Met Gly Tyr Gly Leu Leu Leu Ala Val Cys Phe Asn Glu Gln Ala           
65                  70                  75                  80            

ttg ttt gac gat tta tac cgt tac gta aaa tct cat ttc aat gga aac        288
Leu Phe Asp Asp Leu Tyr Arg Tyr Val Lys Ser His Phe Asn Gly Asn           
                85                  90                  95                

gga ctt atg cac tgg cac att gat gcc aac aac aat gtt aca agt cat        336
Gly Leu Met His Trp His Ile Asp Ala Asn Asn Asn Val Thr Ser His           
            100                 105                 110                   

gac ggc ggc gac ggt gcg gca acc gat gct gat gag gat att gca ctt        384
Asp Gly Gly Asp Gly Ala Ala Thr Asp Ala Asp Glu Asp Ile Ala Leu           
        115                 120                 125                       

gcg ctc ata ttt gcg gac aag tta tgg ggt tct tcc ggt gca ata aac        432
Ala Leu Ile Phe Ala Asp Lys Leu Trp Gly Ser Ser Gly Ala Ile Asn           
    130                 135                 140                           

tac ggg cag gaa gca agg aca ttg ata aac aat ctt tac aac cat tgt        480
Tyr Gly Gln Glu Ala Arg Thr Leu Ile Asn Asn Leu Tyr Asn His Cys           
145                 150                 155                 160           

gta gag cat gga tcc tat gta tta aag ccc ggt gac aga tgg gga ggt        528
Val Glu His Gly Ser Tyr Val Leu Lys Pro Gly Asp Arg Trp Gly Gly           
                165                 170                 175               

tca tca gta aca aac ccg tca tat ttt gcg cct gca tgg tac aaa gtg        576
Ser Ser Val Thr Asn Pro Ser Tyr Phe Ala Pro Ala Trp Tyr Lys Val           
            180                 185                 190                   

tat gct caa tat aca gga gac aca aga tgg aat caa gtg gcg gac aag        624
Tyr Ala Gln Tyr Thr Gly Asp Thr Arg Trp Asn Gln Val Ala Asp Lys           
        195                 200                 205                       

tgt tac caa att gtt gaa gaa gtt aag aaa tac aac aac gga acc ggc        672
Cys Tyr Gln Ile Val Glu Glu Val Lys Lys Tyr Asn Asn Gly Thr Gly           
    210                 215                 220                           

ctt gtt cct gac tgg tgt act gca agc gga act ccg gca agc ggt cag        720
Leu Val Pro Asp Trp Cys Thr Ala Ser Gly Thr Pro Ala Ser Gly Gln           
225                 230                 235                 240           

agt tac gac tac aaa tat gat gct aca cgt tac ggc tgg aga act gcc        768
Ser Tyr Asp Tyr Lys Tyr Asp Ala Thr Arg Tyr Gly Trp Arg Thr Ala           
                245                 250                 255               

gtg gac tat tca tgg ttt ggt gac cag aga gca aag gca aac tgc gat        816
Val Asp Tyr Ser Trp Phe Gly Asp Gln Arg Ala Lys Ala Asn Cys Asp           
            260                 265                 270                   

atg ctg acc aaa ttc ttt gcc aga gac ggg gca aaa gga atc gtt gac        864
Met Leu Thr Lys Phe Phe Ala Arg Asp Gly Ala Lys Gly Ile Val Asp           
        275                 280                 285                       

gga tac aca att caa ggt tca aaa att agc aac aat cac aac gca tca        912
Gly Tyr Thr Ile Gln Gly Ser Lys Ile Ser Asn Asn His Asn Ala Ser           
    290                 295                 300                           

ttt ata gga cct gtt gcg gca gca agt atg aca ggt tac gat ttg aac        960
Phe Ile Gly Pro Val Ala Ala Ala Ser Met Thr Gly Tyr Asp Leu Asn           
305                 310                 315                 320           

ttt gca aag gaa ctt tat agg gag act gtt gct gta aag gac agt gaa       1008
Phe Ala Lys Glu Leu Tyr Arg Glu Thr Val Ala Val Lys Asp Ser Glu           
                325                 330                 335               

tat tac gga tat tac gga aac agc ttg aga ctg ctc act ttg ttg tac       1056
Tyr Tyr Gly Tyr Tyr Gly Asn Ser Leu Arg Leu Leu Thr Leu Leu Tyr           
            340                 345                 350                   

ata aca gga aac ttc ccg aat cct ttg agt gac ctt tcc ggc caa ccg       1104
Ile Thr Gly Asn Phe Pro Asn Pro Leu Ser Asp Leu Ser Gly Gln Pro           
        355                 360                 365                       

aca cca ccg tcg aat ccg aca cct tca ttg cct cct cag gtt gtt tac       1152
Thr Pro Pro Ser Asn Pro Thr Pro Ser Leu Pro Pro Gln Val Val Tyr           
    370                 375                 380                           

ggt gat gta aat ggc gac ggt aat gtt aac tcc act gat ttg act atg       1200
Gly Asp Val Asn Gly Asp Gly Asn Val Asn Ser Thr Asp Leu Thr Met           
385                 390                 395                 400           

tta aaa aga tat ctg ctg aag agt gtt acc aat ata aac aga gag gct       1248
Leu Lys Arg Tyr Leu Leu Lys Ser Val Thr Asn Ile Asn Arg Glu Ala           
                405                 410                 415               

gca gac gtt aat cgt gac ggt gcg att aac tcc tct gac atg act ata       1296
Ala Asp Val Asn Arg Asp Gly Ala Ile Asn Ser Ser Asp Met Thr Ile           
            420                 425                 430                   

tta aag aga tat ctg ata aag agc ata ccc cac cta cct tat tag           1341
Leu Lys Arg Tyr Leu Ile Lys Ser Ile Pro His Leu Pro Tyr                   
        435                 440                 445                       


<210>  8
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Ala Gly Val Pro Phe Asn Thr Lys Tyr Pro Tyr Gly Pro Thr Ser 
1               5                   10                  15      


Ile Ala Asp Asn Gln Ser Glu Val Thr Ala Met Leu Lys Ala Glu Trp 
            20                  25                  30          


Glu Asp Trp Lys Ser Lys Arg Ile Thr Ser Asn Gly Ala Gly Gly Tyr 
        35                  40                  45              


Lys Arg Val Gln Arg Asp Ala Ser Thr Asn Tyr Asp Thr Val Ser Glu 
    50                  55                  60                  


Gly Met Gly Tyr Gly Leu Leu Leu Ala Val Cys Phe Asn Glu Gln Ala 
65                  70                  75                  80  


Leu Phe Asp Asp Leu Tyr Arg Tyr Val Lys Ser His Phe Asn Gly Asn 
                85                  90                  95      


Gly Leu Met His Trp His Ile Asp Ala Asn Asn Asn Val Thr Ser His 
            100                 105                 110         


Asp Gly Gly Asp Gly Ala Ala Thr Asp Ala Asp Glu Asp Ile Ala Leu 
        115                 120                 125             


Ala Leu Ile Phe Ala Asp Lys Leu Trp Gly Ser Ser Gly Ala Ile Asn 
    130                 135                 140                 


Tyr Gly Gln Glu Ala Arg Thr Leu Ile Asn Asn Leu Tyr Asn His Cys 
145                 150                 155                 160 


Val Glu His Gly Ser Tyr Val Leu Lys Pro Gly Asp Arg Trp Gly Gly 
                165                 170                 175     


Ser Ser Val Thr Asn Pro Ser Tyr Phe Ala Pro Ala Trp Tyr Lys Val 
            180                 185                 190         


Tyr Ala Gln Tyr Thr Gly Asp Thr Arg Trp Asn Gln Val Ala Asp Lys 
        195                 200                 205             


Cys Tyr Gln Ile Val Glu Glu Val Lys Lys Tyr Asn Asn Gly Thr Gly 
    210                 215                 220                 


Leu Val Pro Asp Trp Cys Thr Ala Ser Gly Thr Pro Ala Ser Gly Gln 
225                 230                 235                 240 


Ser Tyr Asp Tyr Lys Tyr Asp Ala Thr Arg Tyr Gly Trp Arg Thr Ala 
                245                 250                 255     


Val Asp Tyr Ser Trp Phe Gly Asp Gln Arg Ala Lys Ala Asn Cys Asp 
            260                 265                 270         


Met Leu Thr Lys Phe Phe Ala Arg Asp Gly Ala Lys Gly Ile Val Asp 
        275                 280                 285             


Gly Tyr Thr Ile Gln Gly Ser Lys Ile Ser Asn Asn His Asn Ala Ser 
    290                 295                 300                 


Phe Ile Gly Pro Val Ala Ala Ala Ser Met Thr Gly Tyr Asp Leu Asn 
305                 310                 315                 320 


Phe Ala Lys Glu Leu Tyr Arg Glu Thr Val Ala Val Lys Asp Ser Glu 
                325                 330                 335     


Tyr Tyr Gly Tyr Tyr Gly Asn Ser Leu Arg Leu Leu Thr Leu Leu Tyr 
            340                 345                 350         


Ile Thr Gly Asn Phe Pro Asn Pro Leu Ser Asp Leu Ser Gly Gln Pro 
        355                 360                 365             


Thr Pro Pro Ser Asn Pro Thr Pro Ser Leu Pro Pro Gln Val Val Tyr 
    370                 375                 380                 


Gly Asp Val Asn Gly Asp Gly Asn Val Asn Ser Thr Asp Leu Thr Met 
385                 390                 395                 400 


Leu Lys Arg Tyr Leu Leu Lys Ser Val Thr Asn Ile Asn Arg Glu Ala 
                405                 410                 415     


Ala Asp Val Asn Arg Asp Gly Ala Ile Asn Ser Ser Asp Met Thr Ile 
            420                 425                 430         


Leu Lys Arg Tyr Leu Ile Lys Ser Ile Pro His Leu Pro Tyr 
        435                 440                 445     


