                         SEQUENCE LISTING

<110>  Kapa Biosystems
       McEwan, Paul
       Faurholm, Bjarne
       van der Walt, Eric
 
<120>  Nucleic Acid Amplification

<130>  2006735-0017

<140>  Not Yet Assigned
<141>  2009-12-02

<150>  US 61/119136
<151>  2008-12-02

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  1695
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of Tpol

<400>  1
atggctagcg ccgaaggttt tgaactgcat tatattccgg aagttggtcc gggtatgggt       60

gaactgctgg atctgctgat gcgtcagccg gttctgggtg ttgatctgga aaccaccggt      120

ctggatccgc ataccagccg tccgcgtctg ctgtctctgg ccatgcctgg tgcagttgtt      180

gtttttgacc tgtttggtgt tccgctggaa gttttttatc cgctgtttag ccgtgaagaa      240

ggtccgctgc tggttggtca taatctgaaa tttgatctgc tgtttctgct gaaagcaggt      300

gtttggcgtg caagcggtaa acgtctgtgg gataccggtc tggcccatca ggttctgcat      360

gcacaggcac gtatgcctgc actgaaagat ctggctccgg gtctggataa aaccctgcag      420

accagcgatt ggggtggtcc gctgtctagc gaacaggttg catatgcagc actggatgca      480

gcagttccgc tggttctgta tcgtgaacag cgtgaacgtg cacgtaccct gcgtctggaa      540

aaagttctgg aagttgaacg tcgtgcactg cctgcagttg catggatgga actgcgtggt      600

gttccgtttg caccggaact gtgggaagaa gcagcacgcg aagcagaacg tgaagccgaa      660

gcactgcgtg gtgaactgcc gtttggtgtt aattggaatt ctccggcaca ggttctggcc      720

tatctgaaag gtgaaggtct ggatctgccg gatacccgtg aagataccct ggctggttat      780

cgtgaacatc cgctggttgc aaaactgctg cgttatcgcg aagcagcaaa acgtgttagc      840

acctatggta aagaatgggc caaacatctg aatccggcaa ccggtcgtat tcatccgagc      900

tggcagcaga ttggtgcaga aaccggtcgc atggcatgtc gtaaaccgaa tctgcagcag      960

gttccgcgtg atccggcact gcgtcgtgca tttcgtccga aagaaggtcg tgttatgctg     1020

aaagccgatt ttagccagat tgaactgcgt attgcagcag caattgcaaa agaaggtcgc     1080

atgctgcgcg cctttcgtga aggtaaagat ctgcatgcac tgaccgcaag cctggttctg     1140

ggtaaaccgc tggaagaagt gggtaaagaa gatcgtcagc tggccaaagc actgaatttt     1200

ggtctgctgt atggtctggg tgcagaaggt ctgcgtcgtt acgccctgac cgcatatggt     1260

gttaaactga ccctggaaga agcacagaaa ctgcgcgatg cattttttcg tgcatatccg     1320

gctctgaaac gttggcatcg tagccagccg gaaggtgaag ttgttgttcg taccctgctg     1380

ggtcgtcgtc gtaccaccga tcgttatacc gaaaaactga atacaccggt tcagggcacc     1440

ggtgcagatg gtctgaaaat ggcactggcc ctgctgtggg aaaatcgtgg tctgctgtgg     1500

ggtgcatttc cggttctggc cgttcatgat gaagttgttc tggaagcacc ggaagaaggt     1560

gcaaaagaat atctggaaac cctgaccgca ctgatgcgcc agggtatgga agaagttctg     1620

ggcggcgcag ttccggttga agttgaaggt ggtatttatc gtgattgggg tgcaacaccg     1680

tgggaagagg cctaa                                                      1695


<210>  2
<211>  564
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Tpol

<400>  2

Met Ala Ser Ala Glu Gly Phe Glu Leu His Tyr Ile Pro Glu Val Gly 
1               5                   10                  15      


Pro Gly Met Gly Glu Leu Leu Asp Leu Leu Met Arg Gln Pro Val Leu 
            20                  25                  30          


Gly Val Asp Leu Glu Thr Thr Gly Leu Asp Pro His Thr Ser Arg Pro 
        35                  40                  45              


Arg Leu Leu Ser Leu Ala Met Pro Gly Ala Val Val Val Phe Asp Leu 
    50                  55                  60                  


Phe Gly Val Pro Leu Glu Val Phe Tyr Pro Leu Phe Ser Arg Glu Glu 
65                  70                  75                  80  


Gly Pro Leu Leu Val Gly His Asn Leu Lys Phe Asp Leu Leu Phe Leu 
                85                  90                  95      


Leu Lys Ala Gly Val Trp Arg Ala Ser Gly Lys Arg Leu Trp Asp Thr 
            100                 105                 110         


Gly Leu Ala His Gln Val Leu His Ala Gln Ala Arg Met Pro Ala Leu 
        115                 120                 125             


Lys Asp Leu Ala Pro Gly Leu Asp Lys Thr Leu Gln Thr Ser Asp Trp 
    130                 135                 140                 


Gly Gly Pro Leu Ser Ser Glu Gln Val Ala Tyr Ala Ala Leu Asp Ala 
145                 150                 155                 160 


Ala Val Pro Leu Val Leu Tyr Arg Glu Gln Arg Glu Arg Ala Arg Thr 
                165                 170                 175     


Leu Arg Leu Glu Lys Val Leu Glu Val Glu Arg Arg Ala Leu Pro Ala 
            180                 185                 190         


Val Ala Trp Met Glu Leu Arg Gly Val Pro Phe Ala Pro Glu Leu Trp 
        195                 200                 205             


Glu Glu Ala Ala Arg Glu Ala Glu Arg Glu Ala Glu Ala Leu Arg Gly 
    210                 215                 220                 


Glu Leu Pro Phe Gly Val Asn Trp Asn Ser Pro Ala Gln Val Leu Ala 
225                 230                 235                 240 


Tyr Leu Lys Gly Glu Gly Leu Asp Leu Pro Asp Thr Arg Glu Asp Thr 
                245                 250                 255     


Leu Ala Gly Tyr Arg Glu His Pro Leu Val Ala Lys Leu Leu Arg Tyr 
            260                 265                 270         


Arg Glu Ala Ala Lys Arg Val Ser Thr Tyr Gly Lys Glu Trp Ala Lys 
        275                 280                 285             


His Leu Asn Pro Ala Thr Gly Arg Ile His Pro Ser Trp Gln Gln Ile 
    290                 295                 300                 


Gly Ala Glu Thr Gly Arg Met Ala Cys Arg Lys Pro Asn Leu Gln Gln 
305                 310                 315                 320 


Val Pro Arg Asp Pro Ala Leu Arg Arg Ala Phe Arg Pro Lys Glu Gly 
                325                 330                 335     


Arg Val Met Leu Lys Ala Asp Phe Ser Gln Ile Glu Leu Arg Ile Ala 
            340                 345                 350         


Ala Ala Ile Ala Lys Glu Gly Arg Met Leu Arg Ala Phe Arg Glu Gly 
        355                 360                 365             


Lys Asp Leu His Ala Leu Thr Ala Ser Leu Val Leu Gly Lys Pro Leu 
    370                 375                 380                 


Glu Glu Val Gly Lys Glu Asp Arg Gln Leu Ala Lys Ala Leu Asn Phe 
385                 390                 395                 400 


Gly Leu Leu Tyr Gly Leu Gly Ala Glu Gly Leu Arg Arg Tyr Ala Leu 
                405                 410                 415     


Thr Ala Tyr Gly Val Lys Leu Thr Leu Glu Glu Ala Gln Lys Leu Arg 
            420                 425                 430         


Asp Ala Phe Phe Arg Ala Tyr Pro Ala Leu Lys Arg Trp His Arg Ser 
        435                 440                 445             


Gln Pro Glu Gly Glu Val Val Val Arg Thr Leu Leu Gly Arg Arg Arg 
    450                 455                 460                 


Thr Thr Asp Arg Tyr Thr Glu Lys Leu Asn Thr Pro Val Gln Gly Thr 
465                 470                 475                 480 


Gly Ala Asp Gly Leu Lys Met Ala Leu Ala Leu Leu Trp Glu Asn Arg 
                485                 490                 495     


Gly Leu Leu Trp Gly Ala Phe Pro Val Leu Ala Val His Asp Glu Val 
            500                 505                 510         


Val Leu Glu Ala Pro Glu Glu Gly Ala Lys Glu Tyr Leu Glu Thr Leu 
        515                 520                 525             


Thr Ala Leu Met Arg Gln Gly Met Glu Glu Val Leu Gly Gly Ala Val 
    530                 535                 540                 


Pro Val Glu Val Glu Gly Gly Ile Tyr Arg Asp Trp Gly Ala Thr Pro 
545                 550                 555                 560 


Trp Glu Glu Ala 
                


<210>  3
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of Truncated ORF904 Primase

<400>  3
atggctagcg ccattaataa acgcagcaaa gtgattctgc atggcaatgt gaaaaaaacc       60

cgtcgtaccg gtgtttatat gattagcctg gataatagcg gcaataaaga ttttagcagc      120

aattttagca gcgaacgtat tcgctatgca aaatggtttc tggaacatgg ctttaatatt      180

attccgattg atccggaaag caaaaaaccg gttctgaaag aatggcagaa atatagccat      240

gaaatgccgt ccgatgaaga aaaacagcgc tttctgaaaa tgattgaaga aggctataat      300

tacgcaattc cgggtggtca gaaaggtctg gtgattctgg attttgaaag caaagaaaaa      360

ctgaaagcct ggattggtga aagcgcactg gaagaactgt gtcgtaaaac cctgtgtacc      420

aataccgttc atggtggcat tcatatttat gttctgagca atgatattcc gccgcataaa      480

attaatccgc tgtttgaaga aaatggcaaa ggcattattg atctgcagag ctataatagc      540

tatgttctgg gtctgggtag ctgtgttaat catctgcatt gcaccaccga taaatgtccg      600

tggaaagaac agaattatac cacctgctat accctgtata atgaactgaa agaaattagc      660

aaagtggatc tgaaaagcct gctgcgtttt ctggccgaaa aaggtaaacg tctgggtatt      720

acactgagca aaaccgcaaa agaatggctg gaaggcaaaa aagaagaaga agataccgtt      780

gttgaatttg aagaactgcg caaagaactg gttaaacgtg atagcggtaa accggtggaa      840

aaaattaaag aagaaatttg caccaaaagc ccgccgaaac tgattaaaga aattatttgc      900

gaaaacaaaa cctatgccga tgtgaatatt gatcgtagcc gtggtgattg gcatgttatt      960

ctgtatctga tgaaacatgg tgttaccgat ccggataaaa ttctggaact gctgccgcgt     1020

gatagcaaag caaaagaaaa tgaaaaatgg aatacccaga aatattttgt gattaccctg     1080

agcaaagcat ggtctgtggt gaaaaaatat ctggaagcct aa                        1122


<210>  4
<211>  373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of Truncated ORF904 Primase

<400>  4

Met Ala Ser Ala Ile Asn Lys Arg Ser Lys Val Ile Leu His Gly Asn 
1               5                   10                  15      


Val Lys Lys Thr Arg Arg Thr Gly Val Tyr Met Ile Ser Leu Asp Asn 
            20                  25                  30          


Ser Gly Asn Lys Asp Phe Ser Ser Asn Phe Ser Ser Glu Arg Ile Arg 
        35                  40                  45              


Tyr Ala Lys Trp Phe Leu Glu His Gly Phe Asn Ile Ile Pro Ile Asp 
    50                  55                  60                  


Pro Glu Ser Lys Lys Pro Val Leu Lys Glu Trp Gln Lys Tyr Ser His 
65                  70                  75                  80  


Glu Met Pro Ser Asp Glu Glu Lys Gln Arg Phe Leu Lys Met Ile Glu 
                85                  90                  95      


Glu Gly Tyr Asn Tyr Ala Ile Pro Gly Gly Gln Lys Gly Leu Val Ile 
            100                 105                 110         


Leu Asp Phe Glu Ser Lys Glu Lys Leu Lys Ala Trp Ile Gly Glu Ser 
        115                 120                 125             


Ala Leu Glu Glu Leu Cys Arg Lys Thr Leu Cys Thr Asn Thr Val His 
    130                 135                 140                 


Gly Gly Ile His Ile Tyr Val Leu Ser Asn Asp Ile Pro Pro His Lys 
145                 150                 155                 160 


Ile Asn Pro Leu Phe Glu Glu Asn Gly Lys Gly Ile Ile Asp Leu Gln 
                165                 170                 175     


Ser Tyr Asn Ser Tyr Val Leu Gly Leu Gly Ser Cys Val Asn His Leu 
            180                 185                 190         


His Cys Thr Thr Asp Lys Cys Pro Trp Lys Glu Gln Asn Tyr Thr Thr 
        195                 200                 205             


Cys Tyr Thr Leu Tyr Asn Glu Leu Lys Glu Ile Ser Lys Val Asp Leu 
    210                 215                 220                 


Lys Ser Leu Leu Arg Phe Leu Ala Glu Lys Gly Lys Arg Leu Gly Ile 
225                 230                 235                 240 


Thr Leu Ser Lys Thr Ala Lys Glu Trp Leu Glu Gly Lys Lys Glu Glu 
                245                 250                 255     


Glu Asp Thr Val Val Glu Phe Glu Glu Leu Arg Lys Glu Leu Val Lys 
            260                 265                 270         


Arg Asp Ser Gly Lys Pro Val Glu Lys Ile Lys Glu Glu Ile Cys Thr 
        275                 280                 285             


Lys Ser Pro Pro Lys Leu Ile Lys Glu Ile Ile Cys Glu Asn Lys Thr 
    290                 295                 300                 


Tyr Ala Asp Val Asn Ile Asp Arg Ser Arg Gly Asp Trp His Val Ile 
305                 310                 315                 320 


Leu Tyr Leu Met Lys His Gly Val Thr Asp Pro Asp Lys Ile Leu Glu 
                325                 330                 335     


Leu Leu Pro Arg Asp Ser Lys Ala Lys Glu Asn Glu Lys Trp Asn Thr 
            340                 345                 350         


Gln Lys Tyr Phe Val Ile Thr Leu Ser Lys Ala Trp Ser Val Val Lys 
        355                 360                 365             


Lys Tyr Leu Glu Ala 
    370             


<210>  5
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo M13mp18-R

<400>  5
aacgccaggg ttttcccagt cacgacgttg taaaacgacg                             40


<210>  6
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M13-20 Primer

<400>  6
gtaaaacgac ggccagt                                                      17


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M13 Reverse Primer

<400>  7
ggaaacagct atgaccatg                                                    19


<210>  8
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DnaG-F Primer

<400>  8
attaggtctc agcgcccatc atcatcacca tcatgctgga cgaatcccac gc               52


<210>  9
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DnaG-R Primer

<400>  9
ttaaggtctc atatcattac tttttcgcca gctcctgg                               38


<210>  10
<211>  590
<212>  PRT
<213>  Escherichia coli

<400>  10

Met Ala Ser Ala His His His His His His Ala Gly Arg Ile Pro Arg 
1               5                   10                  15      


Val Phe Ile Asn Asp Leu Leu Ala Arg Thr Asp Ile Val Asp Leu Ile 
            20                  25                  30          


Asp Ala Arg Val Lys Leu Lys Lys Gln Gly Lys Asn Phe His Ala Cys 
        35                  40                  45              


Cys Pro Phe His Asn Glu Lys Thr Pro Ser Phe Thr Val Asn Gly Glu 
    50                  55                  60                  


Lys Gln Phe Tyr His Cys Phe Gly Cys Gly Ala His Gly Asn Ala Ile 
65                  70                  75                  80  


Asp Phe Leu Met Asn Tyr Asp Lys Leu Glu Phe Val Glu Thr Val Glu 
                85                  90                  95      


Glu Leu Ala Ala Met His Asn Leu Glu Val Pro Phe Glu Ala Gly Ser 
            100                 105                 110         


Gly Pro Ser Gln Ile Glu Arg His Gln Arg Gln Thr Leu Tyr Gln Leu 
        115                 120                 125             


Met Asp Gly Leu Asn Thr Phe Tyr Gln Gln Ser Leu Gln Gln Pro Val 
    130                 135                 140                 


Ala Thr Ser Ala Arg Gln Tyr Leu Glu Lys Arg Gly Leu Ser His Glu 
145                 150                 155                 160 


Val Ile Ala Arg Phe Ala Ile Gly Phe Ala Pro Pro Gly Trp Asp Asn 
                165                 170                 175     


Val Leu Lys Arg Phe Gly Gly Asn Pro Glu Asn Arg Gln Ser Leu Ile 
            180                 185                 190         


Asp Ala Gly Met Leu Val Thr Asn Asp Gln Gly Arg Ser Tyr Asp Arg 
        195                 200                 205             


Phe Arg Glu Arg Val Met Phe Pro Ile Arg Asp Lys Arg Gly Arg Val 
    210                 215                 220                 


Ile Gly Phe Gly Gly Arg Val Leu Gly Asn Asp Thr Pro Lys Tyr Leu 
225                 230                 235                 240 


Asn Ser Pro Glu Thr Asp Ile Phe His Lys Gly Arg Gln Leu Tyr Gly 
                245                 250                 255     


Leu Tyr Glu Ala Gln Gln Asp Asn Ala Glu Pro Asn Arg Leu Leu Val 
            260                 265                 270         


Val Glu Gly Tyr Met Asp Val Val Ala Leu Ala Gln Tyr Gly Ile Asn 
        275                 280                 285             


Tyr Ala Val Ala Ser Leu Gly Thr Ser Thr Thr Ala Asp His Ile Gln 
    290                 295                 300                 


Leu Leu Phe Arg Ala Thr Asn Asn Val Ile Cys Cys Tyr Asp Gly Asp 
305                 310                 315                 320 


Arg Ala Gly Arg Asp Ala Ala Trp Arg Ala Leu Glu Thr Ala Leu Pro 
                325                 330                 335     


Tyr Met Thr Asp Gly Arg Gln Leu Arg Phe Met Phe Leu Pro Asp Gly 
            340                 345                 350         


Glu Asp Pro Asp Thr Leu Val Arg Lys Glu Gly Lys Glu Ala Phe Glu 
        355                 360                 365             


Ala Arg Met Glu Gln Ala Met Pro Leu Ser Ala Phe Leu Phe Asn Ser 
    370                 375                 380                 


Leu Met Pro Gln Val Asp Leu Ser Thr Pro Asp Gly Arg Ala Arg Leu 
385                 390                 395                 400 


Ser Thr Leu Ala Leu Pro Leu Ile Ser Gln Val Pro Gly Glu Thr Leu 
                405                 410                 415     


Arg Ile Tyr Leu Arg Gln Glu Leu Gly Asn Lys Leu Gly Ile Leu Asp 
            420                 425                 430         


Asp Ser Gln Leu Glu Arg Leu Met Pro Lys Ala Ala Glu Ser Gly Val 
        435                 440                 445             


Ser Arg Pro Val Pro Gln Leu Lys Arg Thr Thr Met Arg Ile Leu Ile 
    450                 455                 460                 


Gly Leu Leu Val Gln Asn Pro Glu Leu Ala Thr Leu Val Pro Pro Leu 
465                 470                 475                 480 


Glu Asn Leu Asp Glu Asn Lys Leu Pro Gly Leu Gly Leu Phe Arg Glu 
                485                 490                 495     


Leu Val Asn Thr Cys Leu Ser Gln Pro Gly Leu Thr Thr Gly Gln Leu 
            500                 505                 510         


Leu Glu His Tyr Arg Gly Thr Asn Asn Ala Ala Thr Leu Glu Lys Leu 
        515                 520                 525             


Ser Met Trp Asp Asp Ile Ala Asp Lys Asn Ile Ala Glu Gln Thr Phe 
    530                 535                 540                 


Thr Asp Ser Leu Asn His Met Phe Asp Ser Leu Leu Glu Leu Arg Gln 
545                 550                 555                 560 


Glu Glu Leu Ile Ala Arg Glu Arg Thr His Gly Leu Ser Asn Glu Glu 
                565                 570                 575     


Arg Leu Glu Leu Trp Thr Leu Asn Gln Glu Leu Ala Lys Lys 
            580                 585                 590 


<210>  11
<211>  1749
<212>  DNA
<213>  Phage T7

<400>  11
catcatcatc atcaccacga caacagccac gatagcgatt ccgttttcct gtatcacatc       60

ccgtgtgaca attgtggttc ctcagatggc aatagcctgt tctcagacgg tcacaccttt      120

tgctatgtgt gtgagaaatg gaccgccggt aatgaggata cgaaagagcg tgcctctaaa      180

cgtaaaccga gtggcgggaa accaatgacc tataatgtgt ggaacttcgg cgaaagcaat      240

ggtcgttatt ctgccctgac tgcccgtggg attagtaaag aaacctgcca gaaagcgggg      300

tattggatcg ctaaagtgga tggggtgatg tatcaggttg ccgattatcg tgatcagaat      360

gggaacattg tgagtcaaaa agtccgtgac aaagacaaaa acttcaaaac aaccgggagc      420

cataaaagtg acgccctgtt tggtaaacac ctgtggaatg ggggtaagaa aatcgtcgta      480

accgagggtg aaattgatat gctgacagta atggagctgc aggactgtaa atatccggtg      540

gtatcactgg gacatggtgc ttcagctgcc aagaaaacat gtgccgccaa ctatgagtat      600

ttcgaccagt ttgagcaaat catcctgatg ttcgatatgg atgaagccgg tcgtaaagca      660

gtggaagaag ctgcccaggt tctgccagct ggtaaagttc gtgttgctgt actgccgtgt      720

aaagatgcca atgagtgcca cctgaatggt catgatcgtg agatcatgga acaggtctgg      780

aacgctggtc cttggatccc tgatggtgtt gttagcgctc tgtcactgcg tgagcgtatt      840

cgtgagcatc tgtccagcga agaaagtgtt ggtctgctgt ttagtgggtg taccggtatt      900

aatgacaaaa ccctgggtgc tcgtgggggt gaagtgatta tggtgaccag tggtagcggt      960

atgggtaaaa gcacgtttgt tcgccagcaa gcactgcaat ggggtactgc tatgggcaag     1020

aaagtgggtc tggccatgct ggaagagtct gtggaggaaa ccgccgagga tctgattgga     1080

ctgcataacc gtgtacgcct gcgccaaagc gacagcctga aacgtgaaat catcgagaac     1140

gggaaatttg atcagtggtt cgacgaactg ttcgggaatg acacgttcca tctgtatgac     1200

agctttgccg aggcagaaac cgatcgcctg ctggctaaac tggcctatat gcgctctggg     1260

ctgggttgtg acgtgatcat cctggaccat attagcattg tggtgtccgc ttcaggagag     1320

tcagacgagc gtaaaatgat tgataatctg atgaccaaac tgaaaggctt cgccaaatca     1380

acgggcgttg tactggtggt aatctgtcac ctgaaaaacc cggacaaagg caaagcacac     1440

gaagaaggtc gtccggttag tatcaccgat ctgcgtggta gtggtgcgct gcgtcaactg     1500

agcgatacga ttattgctct ggagcgtaac cagcaagggg atatgcctaa tctggttctg     1560

gtccgtattc tgaaatgccg cttcaccggc gatactggta ttgccggcta tatggagtat     1620

aacaaagaga ctggctggct ggaaccgtca tcttatagcg gcgaggagga gtctcattcg     1680

gaaagcacgg attggagcaa cgatactgat ttttgataaa gcgctgcact gagctaatga     1740

tatgagacc                                                             1749


<210>  12
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Heli-K318A-F Primer

<400>  12
gcgtcgacgt ttgttcgcca gcaagca                                           27


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Heli-K318A-R Primer

<400>  13
acccataccg ctaccactgg t                                                 21


<210>  14
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of gp4 K318A

<400>  14

Met Ala Ser Ala His His His His His His Asp Asn Ser His Asp Ser 
1               5                   10                  15      


Asp Ser Val Phe Leu Tyr His Ile Pro Cys Asp Asn Cys Gly Ser Ser 
            20                  25                  30          


Asp Gly Asn Ser Leu Phe Ser Asp Gly His Thr Phe Cys Tyr Val Cys 
        35                  40                  45              


Glu Lys Trp Thr Ala Gly Asn Glu Asp Thr Lys Glu Arg Ala Ser Lys 
    50                  55                  60                  


Arg Lys Pro Ser Gly Gly Lys Pro Met Thr Tyr Asn Val Trp Asn Phe 
65                  70                  75                  80  


Gly Glu Ser Asn Gly Arg Tyr Ser Ala Leu Thr Ala Arg Gly Ile Ser 
                85                  90                  95      


Lys Glu Thr Cys Gln Lys Ala Gly Tyr Trp Ile Ala Lys Val Asp Gly 
            100                 105                 110         


Val Met Tyr Gln Val Ala Asp Tyr Arg Asp Gln Asn Gly Asn Ile Val 
        115                 120                 125             


Ser Gln Lys Val Arg Asp Lys Asp Lys Asn Phe Lys Thr Thr Gly Ser 
    130                 135                 140                 


His Lys Ser Asp Ala Leu Phe Gly Lys His Leu Trp Asn Gly Gly Lys 
145                 150                 155                 160 


Lys Ile Val Val Thr Glu Gly Glu Ile Asp Met Leu Thr Val Met Glu 
                165                 170                 175     


Leu Gln Asp Cys Lys Tyr Pro Val Val Ser Leu Gly His Gly Ala Ser 
            180                 185                 190         


Ala Ala Lys Lys Thr Cys Ala Ala Asn Tyr Glu Tyr Phe Asp Gln Phe 
        195                 200                 205             


Glu Gln Ile Ile Leu Met Phe Asp Met Asp Glu Ala Gly Arg Lys Ala 
    210                 215                 220                 


Val Glu Glu Ala Ala Gln Val Leu Pro Ala Gly Lys Val Arg Val Ala 
225                 230                 235                 240 


Val Leu Pro Cys Lys Asp Ala Asn Glu Cys His Leu Asn Gly His Asp 
                245                 250                 255     


Arg Glu Ile Met Glu Gln Val Trp Asn Ala Gly Pro Trp Ile Pro Asp 
            260                 265                 270         


Gly Val Val Ser Ala Leu Ser Leu Arg Glu Arg Ile Arg Glu His Leu 
        275                 280                 285             


Ser Ser Glu Glu Ser Val Gly Leu Leu Phe Ser Gly Cys Thr Gly Ile 
    290                 295                 300                 


Asn Asp Lys Thr Leu Gly Ala Arg Gly Gly Glu Val Ile Met Val Thr 
305                 310                 315                 320 


Ser Gly Ser Gly Met Gly Ala Ser Thr Phe Val Arg Gln Gln Ala Leu 
                325                 330                 335     


Gln Trp Gly Thr Ala Met Gly Lys Lys Val Gly Leu Ala Met Leu Glu 
            340                 345                 350         


Glu Ser Val Glu Glu Thr Ala Glu Asp Leu Ile Gly Leu His Asn Arg 
        355                 360                 365             


Val Arg Leu Arg Gln Ser Asp Ser Leu Lys Arg Glu Ile Ile Glu Asn 
    370                 375                 380                 


Gly Lys Phe Asp Gln Trp Phe Asp Glu Leu Phe Gly Asn Asp Thr Phe 
385                 390                 395                 400 


His Leu Tyr Asp Ser Phe Ala Glu Ala Glu Thr Asp Arg Leu Leu Ala 
                405                 410                 415     


Lys Leu Ala Tyr Met Arg Ser Gly Leu Gly Cys Asp Val Ile Ile Leu 
            420                 425                 430         


Asp His Ile Ser Ile Val Val Ser Ala Ser Gly Glu Ser Asp Glu Arg 
        435                 440                 445             


Lys Met Ile Asp Asn Leu Met Thr Lys Leu Lys Gly Phe Ala Lys Ser 
    450                 455                 460                 


Thr Gly Val Val Leu Val Val Ile Cys His Leu Lys Asn Pro Asp Lys 
465                 470                 475                 480 


Gly Lys Ala His Glu Glu Gly Arg Pro Val Ser Ile Thr Asp Leu Arg 
                485                 490                 495     


Gly Ser Gly Ala Leu Arg Gln Leu Ser Asp Thr Ile Ile Ala Leu Glu 
            500                 505                 510         


Arg Asn Gln Gln Gly Asp Met Pro Asn Leu Val Leu Val Arg Ile Leu 
        515                 520                 525             


Lys Cys Arg Phe Thr Gly Asp Thr Gly Ile Ala Gly Tyr Met Glu Tyr 
    530                 535                 540                 


Asn Lys Glu Thr Gly Trp Leu Glu Pro Ser Ser Tyr Ser Gly Glu Glu 
545                 550                 555                 560 


Glu Ser His Ser Glu Ser Thr Asp Trp Ser Asn Asp Thr Asp Phe 
                565                 570                 575 


<210>  15
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of Truncated T7 gp4A, HeliTrunc

<400>  15

Met Ala Ser Ala His His His His His His Asp Asn Ser His Asp Ser 
1               5                   10                  15      


Asp Ser Val Phe Leu Tyr His Ile Pro Cys Asp Asn Cys Gly Ser Ser 
            20                  25                  30          


Asp Gly Asn Ser Leu Phe Ser Asp Gly His Thr Phe Cys Tyr Val Cys 
        35                  40                  45              


Glu Lys Trp Thr Ala Gly Asn Glu Asp Thr Lys Glu Arg Ala Ser Lys 
    50                  55                  60                  


Arg Lys Pro Ser Gly Gly Lys Pro Met Thr Tyr Asn Val Trp Asn Phe 
65                  70                  75                  80  


Gly Glu Ser Asn Gly Arg Tyr Ser Ala Leu Thr Ala Arg Gly Ile Ser 
                85                  90                  95      


Lys Glu Thr Cys Gln Lys Ala Gly Tyr Trp Ile Ala Lys Val Asp Gly 
            100                 105                 110         


Val Met Tyr Gln Val Ala Asp Tyr Arg Asp Gln Asn Gly Asn Ile Val 
        115                 120                 125             


Ser Gln Lys Val Arg Asp Lys Asp Lys Asn Phe Lys Thr Thr Gly Ser 
    130                 135                 140                 


His Lys Ser Asp Ala Leu Phe Gly Lys His Leu Trp Asn Gly Gly Lys 
145                 150                 155                 160 


Lys Ile Val Val Thr Glu Gly Glu Ile Asp Met Leu Thr Val Met Glu 
                165                 170                 175     


Leu Gln Asp Cys Lys Tyr Pro Val Val Ser Leu Gly His Gly Ala Ser 
            180                 185                 190         


Ala Ala Lys Lys Thr Cys Ala Ala Asn Tyr Glu Tyr Phe Asp Gln Phe 
        195                 200                 205             


Glu Gln Ile Ile Leu Met Phe Asp Met Asp Glu Ala Gly Arg Lys Ala 
    210                 215                 220                 


Val Glu Glu Ala Ala Gln Val Leu Pro Ala Gly Lys Val Arg Val Ala 
225                 230                 235                 240 


Val Leu Pro Cys Lys Asp Ala Asn Glu Cys His Leu Asn Gly His Asp 
                245                 250                 255     


Arg Glu Ile Met Glu Gln Val Trp Asn Ala Gly Pro Trp Ile Pro Asp 
            260                 265                 270         


Gly Val Val Ser Ala Ala Leu Ser 
        275                 280 


