                         SEQUENCE LISTING

<110>  FARMER, WILLIAM R
       BICKMEIER, JEFF
       LU, CHENFENG
       CHANG, DONG-EUN
       SKRALY, FRANK
       RAMSEIER, TOM M
 
<120>  METHODS AND COMPOSITIONS FOR PRODUCING POLY(5HV)  AND 5 CARBON 
       CHEMICALS

<130>  MBX 067

<150>  US 61/122,250
<151>  2008-12-12

<160>  65    

<170>  PatentIn version 3.3

<210>  1
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic FS-E5' primer

<400>  1
ggaattcagg aggtttttat gaacgatacg gccaacaaga ccagc                     45


<210>  2
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic FS-E3' Primer

<400>  2
ggggtacctc actggccggt ggtgggcttg gtggtcttgc ggcg                      44


<210>  3
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic FS-C5' Primer

<400>  3
ggggtaccag gaggttttta tgtccccatt cccgatcgac atccg                     45


<210>  4
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic FS-C3' Primer

<400>  4
cgggatcctc agccgcgttc gttcagccag cggccgatcg ccg                       43


<210>  5
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic K5-1 5' Primer

<400>  5
gctgaggatc caggaggttt ttatgttagg tcagatgatg cgtaatc                   47


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer K3-1

<400>  6
ctagaggatc cttattcaca gacagaagaa ctactg                               36


<210>  7
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer K5-2

<400>  7
aattcaggag gtttttatgt taggtcagat gatgcgtaat c                         41


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer K3-2

<400>  8
gatccttatt cacagacaga agaactactg                                      30


<210>  9
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer Posynrbs.c

<400>  9
ggaattcagg aggtttttat gttaggtcag atgatgcgta atcag                     45


<210>  10
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer Posynrbs.r

<400>  10
cgggatcctt attcacagac agaagaacta ctgcg                                35


<210>  11
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  Primer A.eut.PhaG.c

<400>  11
ggaattcgga tcccaagtac cttgccgaca tctatgcgct ggc                       43


<210>  12
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer A.eut.EcoRI.r

<400>  12
ggaattcccg gctccgggat tgccctggcc ggact                                35


<210>  13
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS069

<400>  13
ggtggatcct taagaggagg tttttatgac gcgtgaagtg gtagtgg                   47


<210>  14
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS070

<400>  14
ggtgctagct cagatacgct cgaagatggc g                                    31


<210>  15
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS071

<400>  15
ggtcctaggt taagaggagg tttttatgac aacattacaa ggtaaag                   47


<210>  16
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS072

<400>  16
ggtgcggccg cttacatgta taagccgccg ttaat                                35


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB123b

<400>  17
ttatttcatg aaccctcgaa ttgacgcgct c                                    31


<210>  18
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB124a

<400>  18
ttatttcatg agcttatcga taccgtcgac c                                    31


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB134

<400>  19
tgagcggata acaatttcac                                                 20


<210>  20
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB135

<400>  20
aataacacgt caacgcaaaa aggccatccg t                                    31


<210>  21
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB136

<400>  21
tttttcatat gaggaggttt ttatgaacaa gaagaaccgc ca                        42


<210>  22
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB137

<400>  22
ttttttgtac atcagccttt acgcaggtgc a                                    31


<210>  23
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB138

<400>  23
tatatactag taggaggata atatgagcaa aaccaacgaa tc                        42


<210>  24
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB139

<400>  24
tttttgtgca ctcaggcgat ttcagcgaag c                                    31


<210>  25
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JB145

<400>  25
tttttagatc taggaggttt ttatgctgcg tgctgcttct cg                        42


<210>  26
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer JB146

<400>  26
tttttagatc tttagcggaa atagtttgga c                                    31


<210>  27
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer RF314

<400>  27
gcaagccaga gtaaccccgg acgcacgctg cgagcggcac gtagtgtgga tgccttacac     60

gccgcattta atcaataacc ttgagcgatt gtgtaggctg gagctgctt                109


<210>  28
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer RF315

<400>  28
gaatttgccc aacgccacgg ggaatcgcct gactgcggcg ctgcattaac tctttattgc     60

tgttcattcg cattctccag atgggaatta gccatggtcc atatgaatat               110


<210>  29
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  Primer MS220

<400>  29
gcaagagtaa atctgcgtat cttcatacca tgactcataa aggagatacc ccggtgtagg     60

ctggagctgc ttc                                                        73


<210>  30
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS217

<400>  30
accgcaggtc tgaaaagacc tgcgagtata tcagagctga atatgtcgcg catatgaata     60

tcctccttag t                                                          71


<210>  31
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS223

<400>  31
tcgattcgtg aataagtggc ttaatattat tcattttaaa gcaagagtaa atctgcgtat     60

c                                                                     61


<210>  32
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS224

<400>  32
gccactttct actcctggac cgcaggtctg aaaagacctg cgagtatatc agagctg        57


<210>  33
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer DE081

<400>  33
aaaagaattc ttaattaatt ctagaaggag gtttcatatg ttcacgggaa gtattgtc       58


<210>  34
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer DE082

<400>  34
agcgatggct ttgaaatact gatacaaacc ttc                                  33


<210>  35
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer DE083

<400>  35
gaaggtttgt atcagtattt caaagccatc gct                                  33


<210>  36
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer DE084

<400>  36
cccgagctcg tttaaactta attaagacta gttttacagc aaaccggcat gctt           54


<210>  37
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA fragment

<400>  37
tttttctaga ttgacagcta gctcagtcct aggtataatg ctagcactag tgtttaaacc     60

ccc                                                                   63


<210>  38
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer DE118

<400>  38
tgtcccatgt gttgggaggg gcctttttta cctggagata tgactgtgta ggctggagct     60

gcttc                                                                 65


<210>  39
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer DE119

<400>  39
tgtcccatgt gttgggaggg gcctttttta cctggagata tgactgtgta ggctggagct     60

gcttc                                                                 65


<210>  40
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer DE122

<400>  40
gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacgtgta ggctggagct     60

gcttc                                                                 65


<210>  41
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer  DE123

<400>  41
tatttgttaa cagcacgtta ctcgcccgga agccgctctg gcaagatggg aattagccat     60

ggtcc                                                                 65


<210>  42
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer DE106

<400>  42
gtgttttctt attactttca aggtcttgca cttggggcgg ctatggtgta ggctggagct     60

gcttc                                                                 65


<210>  43
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer DE107

<400>  43
ctaactgaac aaggcttgtg catgagcaat accgtctctc gccagatggg aattagccat     60

ggtcc                                                                 65


<210>  44
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS286

<400>  44
ccgtttatgt tgccagacag cgctactgat taagcggatt ttttcgcttt catatgaata     60

tcctccttag t                                                          71


<210>  45
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS287

<400>  45
cgagcagatg atttactaaa aaagtttaac attatcagga gagcattatg gtgtaggctg     60

gagctgcttc                                                            70


<210>  46
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS289

<400>  46
tggctccctg acgttttttt agccacgtat caattatagg tacttccatg gtgtaggctg     60

gagctgcttc                                                            70


<210>  47
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS290

<400>  47
gcagcgcaaa gctgcggatg atgacgagat tactgctgct gtgcagactg catatgaata     60

tcctccttag t                                                          71


<210>  48
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS292

<400>  48
ctcccctgga atgcagggga gcggcaagat taaaccagtt cgttcgggca catatgaata     60

tcctccttag t                                                          71


<210>  49
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer MS293

<400>  49
tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg gtgtaggctg     60

gagctgcttc                                                            70


<210>  50
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer FS011

<400>  50
tcccctagga ttcaggaggt ttttatggag tgggaagaga tatataaa                  48


<210>  51
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer FS008

<400>  51
ccttaagtcg acaaattcta aaatctcttt ttaaattc                             38


<210>  52
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JRG047

<400>  52
ttcaggatcc tgcgcatgct agctatagtt ctagaggta                            39


<210>  53
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JRG048

<400>  53
catacgatag ctcataaaaa cctcctcgca gttagcgaat agaaaagccg ttg            53


<210>  54
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntheti Primer JRG049

<400>  54
gaggaggttt ttatgagcta tcgtatgagc tatcgtatgt ttgattatct ggtgc          55


<210>  55
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer JRG050

<400>  55
tctttcatga actcagaatg cctggcggaa aatcg                                35


<210>  56
<211>  1053
<212>  DNA
<213>  Aspergillus terreus

<400>  56
atgccactgg ttgctcaaaa tccactgcca cgtgctattc tgggtctgat gactttcggt     60

ccgagcgaaa gcaaaggtgc gcgtatcact tccctggatg agtttaacaa gtgcctggat    120

tacttccagc agcagggctt ccaggaaatc gataccgcgc gcatctacgt cggcggtgaa    180

caggaggcat tcacggcgca ggcaaagtgg aaagaacgcg gcctgacgct ggcgactaag    240

tggtatccgc agtacccggg tgcgcacaaa ccggatgtcc tgcgtcagaa cctggagctg    300

tccctgaaag aactgggcac gaaccaggtc gatatcttct atctgcacgc cgcggatcgt    360

tctgtgccgt tcgcggaaac tctggaaact gttaacgaac tgcacaaaga aggcaaattt    420

gttcagctgg gtctgtctaa ctacaccgct ttcgaagtag ctgaaatcgt gaccctgtgt    480

aacgagcgtg gttgggttcg tccgactatc taccaggcga tgtataacgc tatcacccgt    540

aacatcgaaa ctgaactgat cccggcgtgc aagcgttacg gtattgacat tgttatctac    600

aacccactgg cgggtggcct gttcagcggc aaatacaaag cacaggacat cccggctgaa    660

ggtcgttaca gcgaccaatc ttccatgggc cagatgtacc gcaaccgtta ctttaaggac    720

gcaacctttg acgctctgcg cctgatcgaa ccggttgttg cgaagcacgg cctgacgatg    780

ccggaaaccg cgttccgctg ggtccaccac cactccgcac tgaacatgga agatggcggc    840

cgtgacggca tcattctggg tgtaagcagc ctggctcagc tggaaaacaa cctgaaagac    900

attcagaaag gtccgctgcc gcaggaggtt gtagacgtcc tggatcaggc ttggctggtg    960

gctaagccga cggctccaaa ctactggcat ctggacctga aatacacgta cgacacccag   1020

gaagctctgt tcaaaccgaa atctaaggcg taa                                1053


<210>  57
<211>  350
<212>  PRT
<213>  Aspergillus terreus

<400>  57

Met Pro Leu Val Ala Gln Asn Pro Leu Pro Arg Ala Ile Leu Gly Leu 
1               5                   10                  15      


Met Thr Phe Gly Pro Ser Glu Ser Lys Gly Ala Arg Ile Thr Ser Leu 
            20                  25                  30          


Asp Glu Phe Asn Lys Cys Leu Asp Tyr Phe Gln Gln Gln Gly Phe Gln 
        35                  40                  45              


Glu Ile Asp Thr Ala Arg Ile Tyr Val Gly Gly Glu Gln Glu Ala Phe 
    50                  55                  60                  


Thr Ala Gln Ala Lys Trp Lys Glu Arg Gly Leu Thr Leu Ala Thr Lys 
65                  70                  75                  80  


Trp Tyr Pro Gln Tyr Pro Gly Ala His Lys Pro Asp Val Leu Arg Gln 
                85                  90                  95      


Asn Leu Glu Leu Ser Leu Lys Glu Leu Gly Thr Asn Gln Val Asp Ile 
            100                 105                 110         


Phe Tyr Leu His Ala Ala Asp Arg Ser Val Pro Phe Ala Glu Thr Leu 
        115                 120                 125             


Glu Thr Val Asn Glu Leu His Lys Glu Gly Lys Phe Val Gln Leu Gly 
    130                 135                 140                 


Leu Ser Asn Tyr Thr Ala Phe Glu Val Ala Glu Ile Val Thr Leu Cys 
145                 150                 155                 160 


Asn Glu Arg Gly Trp Val Arg Pro Thr Ile Tyr Gln Ala Met Tyr Asn 
                165                 170                 175     


Ala Ile Thr Arg Asn Ile Glu Thr Glu Leu Ile Pro Ala Cys Lys Arg 
            180                 185                 190         


Tyr Gly Ile Asp Ile Val Ile Tyr Asn Pro Leu Ala Gly Gly Leu Phe 
        195                 200                 205             


Ser Gly Lys Tyr Lys Ala Gln Asp Ile Pro Ala Glu Gly Arg Tyr Ser 
    210                 215                 220                 


Asp Gln Ser Ser Met Gly Gln Met Tyr Arg Asn Arg Tyr Phe Lys Asp 
225                 230                 235                 240 


Ala Thr Phe Asp Ala Leu Arg Leu Ile Glu Pro Val Val Ala Lys His 
                245                 250                 255     


Gly Leu Thr Met Pro Glu Thr Ala Phe Arg Trp Val His His His Ser 
            260                 265                 270         


Ala Leu Asn Met Glu Asp Gly Gly Arg Asp Gly Ile Ile Leu Gly Val 
        275                 280                 285             


Ser Ser Leu Ala Gln Leu Glu Asn Asn Leu Lys Asp Ile Gln Lys Gly 
    290                 295                 300                 


Pro Leu Pro Gln Glu Val Val Asp Val Leu Asp Gln Ala Trp Leu Val 
305                 310                 315                 320 


Ala Lys Pro Thr Ala Pro Asn Tyr Trp His Leu Asp Leu Lys Tyr Thr 
                325                 330                 335     


Tyr Asp Thr Gln Glu Ala Leu Phe Lys Pro Lys Ser Lys Ala 
            340                 345                 350 


<210>  58
<211>  870
<212>  DNA
<213>  Arabidopsis thaliana

<400>  58
atggaagtag gttttctggg tctgggcatt atgggtaaag ctatgtccat gaacctgctg     60

aaaaacggtt tcaaagttac cgtgtggaac cgcactctgt ctaaatgtga tgaactggtt    120

gaacacggtg caagcgtgtg cgagtctccg gctgaggtga tcaagaaatg caaatacacg    180

atcgcgatgc tgagcgatcc gtgtgcagct ctgtctgttg ttttcgataa aggcggtgtt    240

ctggaacaga tctgcgaggg taagggctac atcgacatgt ctaccgtcga cgcggaaact    300

agcctgaaaa ttaacgaagc gatcacgggc aaaggtggcc gttttgtaga aggtcctgtt    360

agcggttcca aaaagccggc agaagacggc cagctgatca tcctggcagc aggcgacaaa    420

gcactgttcg aggaatccat cccggccttt gatgtactgg gcaaacgttc cttttatctg    480

ggtcaggtgg gtaacggtgc gaaaatgaaa ctgattgtta acatgatcat gggttctatg    540

atgaacgcgt ttagcgaagg tctggtactg gcagataaaa gcggtctgtc tagcgacacg    600

ctgctggata ttctggatct gggtgctatg acgaatccga tgttcaaagg caaaggtccg    660

tccatgacta aatccagcta cccaccggct ttcccgctga aacaccagca gaaagacatg    720

cgtctggctc tggctctggg cgacgaaaac gctgttagca tgccggtcgc tgcggctgcg    780

aacgaagcct tcaagaaagc ccgtagcctg ggcctgggcg atctggactt ttctgctgtt    840

atcgaagcgg taaaattctc tcgtgaataa                                     870


<210>  59
<211>  289
<212>  PRT
<213>  Arabidopsis thaliana

<400>  59

Met Glu Val Gly Phe Leu Gly Leu Gly Ile Met Gly Lys Ala Met Ser 
1               5                   10                  15      


Met Asn Leu Leu Lys Asn Gly Phe Lys Val Thr Val Trp Asn Arg Thr 
            20                  25                  30          


Leu Ser Lys Cys Asp Glu Leu Val Glu His Gly Ala Ser Val Cys Glu 
        35                  40                  45              


Ser Pro Ala Glu Val Ile Lys Lys Cys Lys Tyr Thr Ile Ala Met Leu 
    50                  55                  60                  


Ser Asp Pro Cys Ala Ala Leu Ser Val Val Phe Asp Lys Gly Gly Val 
65                  70                  75                  80  


Leu Glu Gln Ile Cys Glu Gly Lys Gly Tyr Ile Asp Met Ser Thr Val 
                85                  90                  95      


Asp Ala Glu Thr Ser Leu Lys Ile Asn Glu Ala Ile Thr Gly Lys Gly 
            100                 105                 110         


Gly Arg Phe Val Glu Gly Pro Val Ser Gly Ser Lys Lys Pro Ala Glu 
        115                 120                 125             


Asp Gly Gln Leu Ile Ile Leu Ala Ala Gly Asp Lys Ala Leu Phe Glu 
    130                 135                 140                 


Glu Ser Ile Pro Ala Phe Asp Val Leu Gly Lys Arg Ser Phe Tyr Leu 
145                 150                 155                 160 


Gly Gln Val Gly Asn Gly Ala Lys Met Lys Leu Ile Val Asn Met Ile 
                165                 170                 175     


Met Gly Ser Met Met Asn Ala Phe Ser Glu Gly Leu Val Leu Ala Asp 
            180                 185                 190         


Lys Ser Gly Leu Ser Ser Asp Thr Leu Leu Asp Ile Leu Asp Leu Gly 
        195                 200                 205             


Ala Met Thr Asn Pro Met Phe Lys Gly Lys Gly Pro Ser Met Thr Lys 
    210                 215                 220                 


Ser Ser Tyr Pro Pro Ala Phe Pro Leu Lys His Gln Gln Lys Asp Met 
225                 230                 235                 240 


Arg Leu Ala Leu Ala Leu Gly Asp Glu Asn Ala Val Ser Met Pro Val 
                245                 250                 255     


Ala Ala Ala Ala Asn Glu Ala Phe Lys Lys Ala Arg Ser Leu Gly Leu 
            260                 265                 270         


Gly Asp Leu Asp Phe Ser Ala Val Ile Glu Ala Val Lys Phe Ser Arg 
        275                 280                 285             


Glu 
    


<210>  60
<211>  1680
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Pseudomonas putida/Zoogloea ramigera 
       polyhydroxyalkanoate synthase fusion gene phaC3/C5

<400>  60
atgagtaaca agaacaacga tgagctgcag tggcaatcct ggttcagcaa ggcgcccacc     60

accgaggcga acccgatggc caccatgttg caggatatcg gcgttgcgct caaaccggaa    120

gcgatggagc agctgaaaaa cgattatctg cgtgacttca ccgcgttgtg gcaggatttt    180

ttggctggca aggcgccagc cgtcagcgac cgccgcttca gctcggcagc ctggcagggc    240

aatccgatgt cggccttcaa tgccgcatct tacctgctca acgccaaatt cctcagtgcc    300

atggtggagg cggtggacac cgcaccccag caaaagcaga aaatacgctt tgccgtgcag    360

caggtgattg atgccatgtc gcccgcgaac ttcctcgcca ccaacccgga agcgcagcaa    420

aaactgattg aaaccaaggg cgagagcctg acgcgtggcc tggtcaatat gctgggcgat    480

atcaacaagg gccatatctc gctgtcggac gaatcggcct ttgaagtggg ccgcaacctg    540

gccattaccc cgggcaccgt gatttacgaa aatccgctgt tccagctgat ccagtacacg    600

ccgaccacgc cgacggtcag ccagcgcccg ctgttgatgg tgccgccgtg catcaacaag    660

ttctacatcc tcgacctgca accggaaaat tcgctggtgc gctacgcggt ggagcagggc    720

aacaccgtgt tcctgatctc gtggagcaat ccggacaagt cgctggccgg caccacctgg    780

gacgactacg tggagcaggg cgtgatcgaa gcgatccgca tcgtccagga cgtcagcggc    840

caggacaagc tgaacatgtt cggcttctgc gtgggcggca ccatcgttgc caccgcactg    900

gcggtactgg cggcgcgtgg ccagcacccg gcggccagcc tgaccctgct gaccaccttc    960

ctcgacttca gcgacaccgg cgtgctcgac gtcttcgtcg atgaaaccca ggtcgcgctg   1020

cgtgaacagc aattgcgcga tggcggcctg atgccgggcc gtgacctggc ctcgaccttc   1080

tcgagcctgc gtccgaacga cctggtatgg aactatgtgc agtcgaacta cctcaaaggc   1140

aatgagccgg cggcgtttga cctgctgttc tggaattcgg acagcaccaa tttgccgggc   1200

ccgatgttct gctggtacct gcgcaacacc tacctggaaa acagcctgaa agtgccgggc   1260

aagctgacgg tggccggcga aaagatcgac ctcggcctga tcgacgcccc ggccttcatc   1320

tacggttcgc gcgaagacca catcgtgccg tggatgtcgg cgtacggttc gctcgacatc   1380

ctcaaccagg gcaagccggg cgccaaccgc ttcgtgctgg gcgcgtccgg ccatatcgcc   1440

ggcgtgatca actcggtggc caagaacaag cgcagctact ggatcaacga cggtggcgcc   1500

gccgatgccc aggcctggtt cgatggcgcg caggaagtgc cgggcagctg gtggccgcaa   1560

tgggccgggt tcctgaccca gcatggcggc aagaaggtca agcccaaggc caagcccggc   1620

aacgcccgct acaccgcgat cgaggcggcg cccggccgtt acgtcaaagc caagggctga   1680


<210>  61
<211>  559
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Amino Acid Sequence: Pseudomonas putida/Zoogloea 
       ramigera polyhydroxyalkanoate synthase fusion gene PhaC3/C5

<400>  61

Met Ser Asn Lys Asn Asn Asp Glu Leu Gln Trp Gln Ser Trp Phe Ser 
1               5                   10                  15      


Lys Ala Pro Thr Thr Glu Ala Asn Pro Met Ala Thr Met Leu Gln Asp 
            20                  25                  30          


Ile Gly Val Ala Leu Lys Pro Glu Ala Met Glu Gln Leu Lys Asn Asp 
        35                  40                  45              


Tyr Leu Arg Asp Phe Thr Ala Leu Trp Gln Asp Phe Leu Ala Gly Lys 
    50                  55                  60                  


Ala Pro Ala Val Ser Asp Arg Arg Phe Ser Ser Ala Ala Trp Gln Gly 
65                  70                  75                  80  


Asn Pro Met Ser Ala Phe Asn Ala Ala Ser Tyr Leu Leu Asn Ala Lys 
                85                  90                  95      


Phe Leu Ser Ala Met Val Glu Ala Val Asp Thr Ala Pro Gln Gln Lys 
            100                 105                 110         


Gln Lys Ile Arg Phe Ala Val Gln Gln Val Ile Asp Ala Met Ser Pro 
        115                 120                 125             


Ala Asn Phe Leu Ala Thr Asn Pro Glu Ala Gln Gln Lys Leu Ile Glu 
    130                 135                 140                 


Thr Lys Gly Glu Ser Leu Thr Arg Gly Leu Val Asn Met Leu Gly Asp 
145                 150                 155                 160 


Ile Asn Lys Gly His Ile Ser Leu Ser Asp Glu Ser Ala Phe Glu Val 
                165                 170                 175     


Gly Arg Asn Leu Ala Ile Thr Pro Gly Thr Val Ile Tyr Glu Asn Pro 
            180                 185                 190         


Leu Phe Gln Leu Ile Gln Tyr Thr Pro Thr Thr Pro Thr Val Ser Gln 
        195                 200                 205             


Arg Pro Leu Leu Met Val Pro Pro Cys Ile Asn Lys Phe Tyr Ile Leu 
    210                 215                 220                 


Asp Leu Gln Pro Glu Asn Ser Leu Val Arg Tyr Ala Val Glu Gln Gly 
225                 230                 235                 240 


Asn Thr Val Phe Leu Ile Ser Trp Ser Asn Pro Asp Lys Ser Leu Ala 
                245                 250                 255     


Gly Thr Thr Trp Asp Asp Tyr Val Glu Gln Gly Val Ile Glu Ala Ile 
            260                 265                 270         


Arg Ile Val Gln Asp Val Ser Gly Gln Asp Lys Leu Asn Met Phe Gly 
        275                 280                 285             


Phe Cys Val Gly Gly Thr Ile Val Ala Thr Ala Leu Ala Val Leu Ala 
    290                 295                 300                 


Ala Arg Gly Gln His Pro Ala Ala Ser Leu Thr Leu Leu Thr Thr Phe 
305                 310                 315                 320 


Leu Asp Phe Ser Asp Thr Gly Val Leu Asp Val Phe Val Asp Glu Thr 
                325                 330                 335     


Gln Val Ala Leu Arg Glu Gln Gln Leu Arg Asp Gly Gly Leu Met Pro 
            340                 345                 350         


Gly Arg Asp Leu Ala Ser Thr Phe Ser Ser Leu Arg Pro Asn Asp Leu 
        355                 360                 365             


Val Trp Asn Tyr Val Gln Ser Asn Tyr Leu Lys Gly Asn Glu Pro Ala 
    370                 375                 380                 


Ala Phe Asp Leu Leu Phe Trp Asn Ser Asp Ser Thr Asn Leu Pro Gly 
385                 390                 395                 400 


Pro Met Phe Cys Trp Tyr Leu Arg Asn Thr Tyr Leu Glu Asn Ser Leu 
                405                 410                 415     


Lys Val Pro Gly Lys Leu Thr Val Ala Gly Glu Lys Ile Asp Leu Gly 
            420                 425                 430         


Leu Ile Asp Ala Pro Ala Phe Ile Tyr Gly Ser Arg Glu Asp His Ile 
        435                 440                 445             


Val Pro Trp Met Ser Ala Tyr Gly Ser Leu Asp Ile Leu Asn Gln Gly 
    450                 455                 460                 


Lys Pro Gly Ala Asn Arg Phe Val Leu Gly Ala Ser Gly His Ile Ala 
465                 470                 475                 480 


Gly Val Ile Asn Ser Val Ala Lys Asn Lys Arg Ser Tyr Trp Ile Asn 
                485                 490                 495     


Asp Gly Gly Ala Ala Asp Ala Gln Ala Trp Phe Asp Gly Ala Gln Glu 
            500                 505                 510         


Val Pro Gly Ser Trp Trp Pro Gln Trp Ala Gly Phe Leu Thr Gln His 
        515                 520                 525             


Gly Gly Lys Lys Val Lys Pro Lys Ala Lys Pro Gly Asn Ala Arg Tyr 
    530                 535                 540                 


Thr Ala Ile Glu Ala Ala Pro Gly Arg Tyr Val Lys Ala Lys Gly 
545                 550                 555                 


<210>  62
<211>  1152
<212>  DNA
<213>  Thiocapsa phenigii

<400>  62
atggctggtg accacgtcgt ggaatgcctt cgaattcagg aggtttttat gaacgatacg     60

gccaacaaga ccagcgactg gctggacatc caacgcaagt actgggagac ctggtcggag    120

ctcggccgca agaccttggg tctggagaag accccggcca atccttgggc cggcgccctc    180

gatcattggt ggcagacggt ctcgcccgcc gcccccaacg acctggttcg cgacttcatg    240

gagaagctcg ccgagcaggg caaggccttc ttcggcctca ccgactactt cacgaagggc    300

ctcggcggca gtagcggtac gcagggctgg gacaccctct cgaagaccat cgacgacatg    360

caaaaggcct tcgccagcgg ccggatcgaa ggcgacgaga ccttccgccg cctgatggcc    420

ttctgggaga tgccgctcga caactggcag cgcaccatgt cctcgctgtc cccggtgccc    480

ggcgacctgc tgcgcaacat gccgcacgac caagtcaggg acagcgtcga ccgcatcctc    540

tcggcacccg ggctcggcta cacgcgcgag gagcaggccc gctaccagga tctgatccgc    600

cgctcgctgg agtaccagtc ggccctgaac gaatacaacg gcttcttcgg ccagctcggt    660

gtcaagtccc tcgagcggat gcgcgccttc ctgcagggac aggccgagaa gggcgtcgcc    720

atcgagtcgg cgcgcaccct ctacgacgcc tgggtcggct gctgcgaaga ggtctatgcc    780

gaggaggtca gctccgccga ctacgcgcac atccacggcc gcctcgtcaa cgcccagatg    840

gccctcaagc agcgcatgtc gaccatggtc gacgaggtcc tcggcgcgat gccgctgccg    900

acccgcagcg agctgcgcac gctccaggat cggctccagg agtcgcgcgg cgagggcaag    960

cgccagcgcc aagagatcga gacgctgaag cggcaggtcg cggccttggc cggcggcgcc   1020

cagcccgcgc cccaggcctc cgcccagccc agcacccggc ccgcgccggc gacggccccg   1080

gcggcgagcg cggcgcccaa gcgcagcacc acgacccgcc gcaagaccac caagcccacc   1140

accggccagt ga                                                       1152


<210>  63
<211>  367
<212>  PRT
<213>  Thiocapsa phenigii

<400>  63

Met Asn Asp Thr Ala Asn Lys Thr Ser Asp Trp Leu Asp Ile Gln Arg 
1               5                   10                  15      


Lys Tyr Trp Glu Thr Trp Ser Glu Leu Gly Arg Lys Thr Leu Gly Leu 
            20                  25                  30          


Glu Lys Thr Pro Ala Asn Pro Trp Ala Gly Ala Leu Asp His Trp Trp 
        35                  40                  45              


Gln Thr Val Ser Pro Ala Ala Pro Asn Asp Leu Val Arg Asp Phe Met 
    50                  55                  60                  


Glu Lys Leu Ala Glu Gln Gly Lys Ala Phe Phe Gly Leu Thr Asp Tyr 
65                  70                  75                  80  


Phe Thr Lys Gly Leu Gly Gly Ser Ser Gly Thr Gln Gly Trp Asp Thr 
                85                  90                  95      


Leu Ser Lys Thr Ile Asp Asp Met Gln Lys Ala Phe Ala Ser Gly Arg 
            100                 105                 110         


Ile Glu Gly Asp Glu Thr Phe Arg Arg Leu Met Ala Phe Trp Glu Met 
        115                 120                 125             


Pro Leu Asp Asn Trp Gln Arg Thr Met Ser Ser Leu Ser Pro Val Pro 
    130                 135                 140                 


Gly Asp Leu Leu Arg Asn Met Pro His Asp Gln Val Arg Asp Ser Val 
145                 150                 155                 160 


Asp Arg Ile Leu Ser Ala Pro Gly Leu Gly Tyr Thr Arg Glu Glu Gln 
                165                 170                 175     


Ala Arg Tyr Gln Asp Leu Ile Arg Arg Ser Leu Glu Tyr Gln Ser Ala 
            180                 185                 190         


Leu Asn Glu Tyr Asn Gly Phe Phe Gly Gln Leu Gly Val Lys Ser Leu 
        195                 200                 205             


Glu Arg Met Arg Ala Phe Leu Gln Gly Gln Ala Glu Lys Gly Val Ala 
    210                 215                 220                 


Ile Glu Ser Ala Arg Thr Leu Tyr Asp Ala Trp Val Gly Cys Cys Glu 
225                 230                 235                 240 


Glu Val Tyr Ala Glu Glu Val Ser Ser Ala Asp Tyr Ala His Ile His 
                245                 250                 255     


Gly Arg Leu Val Asn Ala Gln Met Ala Leu Lys Gln Arg Met Ser Thr 
            260                 265                 270         


Met Val Asp Glu Val Leu Gly Ala Met Pro Leu Pro Thr Arg Ser Glu 
        275                 280                 285             


Leu Arg Thr Leu Gln Asp Arg Leu Gln Glu Ser Arg Gly Glu Gly Lys 
    290                 295                 300                 


Arg Gln Arg Gln Glu Ile Glu Thr Leu Lys Arg Gln Val Ala Ala Leu 
305                 310                 315                 320 


Ala Gly Gly Ala Gln Pro Ala Pro Gln Ala Ser Ala Gln Pro Ser Thr 
                325                 330                 335     


Arg Pro Ala Pro Ala Thr Ala Pro Ala Ala Ser Ala Ala Pro Lys Arg 
            340                 345                 350         


Ser Thr Thr Thr Arg Arg Lys Thr Thr Lys Pro Thr Thr Gly Gln 
        355                 360                 365         


<210>  64
<211>  1074
<212>  DNA
<213>  Thiocapsa phenigii

<400>  64
atgtccccat tcccgatcga catccggccc gacaagctga ccgaggagat gctggagtac     60

agccgcaagc tcggcgaggg tatgcagaac ctgctcaagg ccgaccagat cgacacaggc    120

gtcaccccca aggacgtcgt ccaccgcgag gacaagctgg tcctctaccg ctaccggcgc    180

ccggcgcagg tggcgaccca gacgatcccg ctgctgatcg tctacgccct cgtcaatcgg    240

ccctacatga ccgacatcca ggaggatcgc tcgacgatca agggcctgct cgccaccggt    300

caggacgtct atctgatcga ctggggctac ccggatcagg ccgaccgggc gctgaccctc    360

gatgactaca tcaacggcta catcgaccgc tgcgtcgact acctgcgcga gacccacggc    420

gtcgaccagg tcaacctgct cgggatctgc cagggcgggg ccttcagcct ctgctacacg    480

gccctgcact ccgagaaggt caaaaacctc gtcaccatgg tcacgccggt cgacttccag    540

accccgggca acctgctctc ggcctgggtc cagaacgtcg acgtcgacct ggccgtcgac    600

accatgggca acatcccggg cgaactgctc aactggacct tcctgtcgct caagcccttc    660

agcctgaccg gccagaagta cgtcaacatg gtcgacctgc tcgacgacga ggacaaggtc    720

aagaacttcc tgcggatgga gaagtggatc ttcgacagcc cggaccaggc cggcgagacc    780

ttccgccagt tcatcaagga cttctaccag cgcaacggct tcatcaacgg cggcgtcctg    840

atcggcgatc aggaggtcga cctgcgcaac atccgctgcc cggtcctgaa catctacccg    900

atgcaggacc acctggtgcc gccggatgcc tccaaggccc tcgcgggact gacctccagc    960

gaggactaca cggagctcgc cttccccggc gggcacatcg gcatctacgt cagcggcaag   1020

gcgcaggaag gagtcacccc ggcgatcggc cgctggctga acgaacgcgg ctga         1074


<210>  65
<211>  357
<212>  PRT
<213>  Thiocapsa phenigii

<400>  65

Met Ser Pro Phe Pro Ile Asp Ile Arg Pro Asp Lys Leu Thr Glu Glu 
1               5                   10                  15      


Met Leu Glu Tyr Ser Arg Lys Leu Gly Glu Gly Met Gln Asn Leu Leu 
            20                  25                  30          


Lys Ala Asp Gln Ile Asp Thr Gly Val Thr Pro Lys Asp Val Val His 
        35                  40                  45              


Arg Glu Asp Lys Leu Val Leu Tyr Arg Tyr Arg Arg Pro Ala Gln Val 
    50                  55                  60                  


Ala Thr Gln Thr Ile Pro Leu Leu Ile Val Tyr Ala Leu Val Asn Arg 
65                  70                  75                  80  


Pro Tyr Met Thr Asp Ile Gln Glu Asp Arg Ser Thr Ile Lys Gly Leu 
                85                  90                  95      


Leu Ala Thr Gly Gln Asp Val Tyr Leu Ile Asp Trp Gly Tyr Pro Asp 
            100                 105                 110         


Gln Ala Asp Arg Ala Leu Thr Leu Asp Asp Tyr Ile Asn Gly Tyr Ile 
        115                 120                 125             


Asp Arg Cys Val Asp Tyr Leu Arg Glu Thr His Gly Val Asp Gln Val 
    130                 135                 140                 


Asn Leu Leu Gly Ile Cys Gln Gly Gly Ala Phe Ser Leu Cys Tyr Thr 
145                 150                 155                 160 


Ala Leu His Ser Glu Lys Val Lys Asn Leu Val Thr Met Val Thr Pro 
                165                 170                 175     


Val Asp Phe Gln Thr Pro Gly Asn Leu Leu Ser Ala Trp Val Gln Asn 
            180                 185                 190         


Val Asp Val Asp Leu Ala Val Asp Thr Met Gly Asn Ile Pro Gly Glu 
        195                 200                 205             


Leu Leu Asn Trp Thr Phe Leu Ser Leu Lys Pro Phe Ser Leu Thr Gly 
    210                 215                 220                 


Gln Lys Tyr Val Asn Met Val Asp Leu Leu Asp Asp Glu Asp Lys Val 
225                 230                 235                 240 


Lys Asn Phe Leu Arg Met Glu Lys Trp Ile Phe Asp Ser Pro Asp Gln 
                245                 250                 255     


Ala Gly Glu Thr Phe Arg Gln Phe Ile Lys Asp Phe Tyr Gln Arg Asn 
            260                 265                 270         


Gly Phe Ile Asn Gly Gly Val Leu Ile Gly Asp Gln Glu Val Asp Leu 
        275                 280                 285             


Arg Asn Ile Arg Cys Pro Val Leu Asn Ile Tyr Pro Met Gln Asp His 
    290                 295                 300                 


Leu Val Pro Pro Asp Ala Ser Lys Ala Leu Ala Gly Leu Thr Ser Ser 
305                 310                 315                 320 


Glu Asp Tyr Thr Glu Leu Ala Phe Pro Gly Gly His Ile Gly Ile Tyr 
                325                 330                 335     


Val Ser Gly Lys Ala Gln Glu Gly Val Thr Pro Ala Ile Gly Arg Trp 
            340                 345                 350         


Leu Asn Glu Arg Gly 
        355         


