                         SEQUENCE LISTING

<110>  ImClone LLC
       LLC, ImClone
 
<120>  ANTIBODY HUMANIZATION

<130>  P-71441-PC

<140>  PCT/US09/999999
<141>  2009-12-10

<150>  61/138,790
<151>  2008-12-18

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  119
<212>  PRT
<213>  Homo sapiens

<400>  1

Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Leu Val Gln Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Asn Tyr 
            20                  25                  30          


Gly His His Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ser Gly Gly Asn Thr Asp Tyr Asn Thr Pro Phe Thr 
    50                  55                  60                  


Ser Arg Leu Ser Ile Asn Lys Asp Asn Ser Lys Ser Gln Val Phe Phe 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Ser Asn Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Ala Leu Thr Tyr Tyr Asp Tyr Glu Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  2
<211>  118
<212>  PRT
<213>  Homo sapiens

<400>  2

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Asn Tyr 
            20                  25                  30          


Gly Val His Trp Ile Arg Gln Pro Pro Gly Lys Leu Glu Trp Ile Gly 
        35                  40                  45              


Val Ile Trp Ser Gly Gly Asn Thr Asp Tyr Asn Thr Pro Phe Thr Ser 
    50                  55                  60                  


Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser Leu Lys 
65                  70                  75                  80  


Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
                85                  90                  95      


Ala Leu Thr Tyr Tyr Asp Tyr Glu Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  3
<211>  119
<212>  PRT
<213>  Homo sapiens

<400>  3

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Asn Tyr 
            20                  25                  30          


Gly Val Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Trp Ser Gly Gly Asn Thr Asn Tyr Asn Pro Ser Leu Lys 
    50                  55                  60                  


Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser Leu 
65                  70                  75                  80  


Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Ala Leu Thr Tyr Tyr Asp Tyr Glu Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  4
<211>  119
<212>  PRT
<213>  Homo sapiens

<400>  4

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser Tyr 
            20                  25                  30          


Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys 
    50                  55                  60                  


Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser Leu 
65                  70                  75                  80  


Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Ala Glu Ala Ala Ala Pro Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  5
<211>  106
<212>  PRT
<213>  Homo sapiens

<400>  5

Asp Ile Leu Leu Thr Gln Ser Pro Val Ile Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Val Ser Phe Ser Cys Arg Ala Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Arg Thr Asn Gly Ser Pro Arg Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Glu Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser Val Glu Ser 
65                  70                  75                  80  


Glu Asp Ile Ala Asp Tyr Cys Gln Gln Asn Asn Asn Trp Pro Thr Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105     


<210>  6
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  6

Glu Thr Thr Leu Thr Gln Ser Pro Gly Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Thr Thr Leu Ser Cys Arg Ala Ser Glu Ser Ile Arg Asp Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Thr Arg Ala Thr Gly Val Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Tyr Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Ser Ala Val Tyr Tyr Cys Gln Gln Tyr Ser Asn Trp Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  7
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  7

Glu Thr Thr Leu Thr Gln Ser Pro Ala Thr Leu Ser Met Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Glu Ser Val Thr Thr Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Lys Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Thr Arg Ala Thr Asp Thr Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Ala Asp Phe Ala Ile Tyr Tyr Cys Gln Gln Tyr Lys Asn Trp Pro Val 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Thr Val Glu Ile Lys 
            100                 105         


<210>  8
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  8

Glu Thr Thr Leu Thr Gln Ser Pro Val Thr Leu Ser Met Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Glu Ser Val Thr Thr Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Lys Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Thr Arg Ala Thr Asp Thr Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Ala Asp Phe Ala Ile Tyr Tyr Cys Gln Gln Tyr Lys Asn Trp Pro Val 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Thr Val Glu Ile Lys 
            100                 105         


<210>  9
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  9

Glu Thr Thr Leu Thr Gln Ser Pro Val Thr Leu Ala Leu Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Glu Ser Val Thr Thr Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Lys Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Thr Arg Ala Thr Asp Thr Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Ala Asp Phe Ala Ile Tyr Tyr Cys Gln Gln Tyr Lys Asn Trp Pro Val 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Thr Val Glu Ile Lys 
            100                 105         


<210>  10
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  10

Glu Thr Thr Leu Thr Gln Ser Pro Val Thr Leu Ser Met Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Glu Ser Val Thr Thr Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Lys Lys Pro Gly Gln Pro Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Thr Arg Ala Thr Asp Thr Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Ala Asp Phe Ala Ile Tyr Tyr Cys Gln Gln Tyr Lys Asn Trp Pro Val 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Thr Val Glu Ile Lys 
            100                 105         


<210>  11
<211>  108
<212>  PRT
<213>  Homo sapiens

<400>  11

Glu Thr Thr Leu Thr Gln Ser Pro Pro Thr Leu Ala Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Thr Val Ser Asn Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Asp Thr Ser Thr Arg Ala Thr Gly Phe Pro Ala Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Val Phe Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Ser Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Asn Asn Trp Pro 
                85                  90                  95      


Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105             


<210>  12
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  12

Glu Thr Thr Leu Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly Glu 
1               5                   10                  15      


Arg Val Thr Leu Ser Cys Arg Ala Ser Ser Val Tyr Ser Asn Ile Ala 
            20                  25                  30          


Trp Tyr Lys Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              


Thr Ala Ser Arg Thr Arg Ala Thr Gly Val Pro Ala Arg Phe Ser Ala 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Ser Ala Val Tyr Tyr Cys Gln Gln Tyr Ser Asn Trp Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  13
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  13

Glu Thr Thr Leu Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Asn Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Phe Gly Ala Ser Thr Arg Ala Thr Gly Val Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Ser Ala Val Tyr Ser Cys Gln Gln Tyr Asn Asn Trp Pro Leu 
                85                  90                  95      


Thr Phe Ala Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  14
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  14

Glu Thr Thr Leu Thr Gln Ser Pro Gly Thr Leu Ser Ser Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Thr Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Thr Gly Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Ser Ala Val Tyr Tyr Cys Gln Gln Tyr Asn Asn Trp Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  15
<211>  106
<212>  PRT
<213>  Homo sapiens

<400>  15

Glu Thr Thr Leu Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Thr Thr Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Thr Arg Ala Thr Gly Val Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Asn Trp Pro Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105     


<210>  16
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  16

Arg Ala Ser Gln Ser Ile Gly Thr Asn Ile His Tyr Ala Ser Glu Ser 
1               5                   10                  15      


Ile Ser Gln Gln Asn Asn Asn Trp Pro Thr Thr 
            20                  25          


<210>  17
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  17

Arg Ala Ser Glu Ser Ile Arg Asp Asn Leu Ala Asp Ala Ser Thr Arg 
1               5                   10                  15      


Ala Thr Gln Gln Tyr Ser Asn Trp Pro Leu Thr 
            20                  25          


<210>  18
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  18

Arg Ala Ser Glu Ser Val Thr Thr Asn Leu Ala Asp Ala Ser Thr Arg 
1               5                   10                  15      


Ala Thr Gln Gln Tyr Lys Asn Trp Pro Val Thr 
            20                  25          


<210>  19
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  19

Arg Ala Ser Gln Thr Val Ser Asn Asn Leu Ala Asp Thr Ser Thr Arg 
1               5                   10                  15      


Ala Thr Gln Gln Tyr Asn Asn Trp Pro Leu Thr 
            20                  25          


<210>  20
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  20

Arg Ala Ser Gln Ser Val Tyr Ser Asn Ile Ala Thr Ala Ser Thr Arg 
1               5                   10                  15      


Ala Thr Gln Gln Tyr Ser Asn Trp Pro Leu Thr 
            20                  25          


<210>  21
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  21

Arg Ala Ser Gln Ser Val Ser Asn Asn Leu Ala Gly Ala Ser Thr Arg 
1               5                   10                  15      


Ala Thr Gln Gln Tyr Asn Asn Trp Pro Leu Thr 
            20                  25          


<210>  22
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  22

Arg Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Gly Ala Ser Thr Arg 
1               5                   10                  15      


Ala Thr Gln Gln Tyr Asn Asn Trp Pro Leu Thr 
            20                  25          


<210>  23
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  23

Arg Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Gly Ala Ser Thr Arg 
1               5                   10                  15      


Ala Thr Gln Gln Tyr Asn Asn Trp Pro Leu Thr 
            20                  25          


