                         SEQUENCE LISTING

<110>  DiMarchi, Richard
       Jie, Han
 
<120>  Insulin Analogs

<130>  29920-210273

<140>  PCT/US2009/068712
<141>  2009-12-18

<150>  US 61/139,221
<151>  2008-12-19

<160>  52    

<170>  PatentIn version 3.3

<210>  1
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  1

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  2
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  2

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is threonine or histidine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is 4-amino phenylalanine or 4-methoxy 
       phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is asparagine, glycine, alanine, glutamine, 
       glutamate, threonine, or serine

<400>  3

Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Xaa 
            20      


<210>  4
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  4

Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  5
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine and threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is threonine or alanine

<400>  5

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Lys Xaa 
            20                  25                  30  


<210>  6
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin a chain


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is 4-amino phenylalanine

<400>  6

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Asn 
            20      


<210>  7
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  7

His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  8
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  8

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  9
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  9

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr 
            20                  25                  30  


<210>  10
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  10

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  11

Gly Gly Gly Pro Gly Lys Arg 
1               5           


<210>  12
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  12

Gly Tyr Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10          


<210>  13
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  13

Gly Gly Gly Gly Gly Lys Arg 
1               5           


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  14

Arg Arg Gly Gly Gly Gly Gly 
1               5           


<210>  15
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  15

Gly Gly Ala Pro Gly Asp Val Lys Arg 
1               5                   


<210>  16
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  16

Arg Arg Ala Pro Gly Asp Val Gly Gly 
1               5                   


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  17

Gly Gly Tyr Pro Gly Asp Val Lys Arg 
1               5                   


<210>  18
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  18

Arg Arg Tyr Pro Gly Asp Val Gly Gly 
1               5                   


<210>  19
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  19

Gly Gly His Pro Gly Asp Val Lys Arg 
1               5                   


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  20

Arg Arg His Pro Gly Asp Val Gly Gly 
1               5                   


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  21

Arg Arg Gly Pro Gly Gly Gly 
1               5           


<210>  22
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  single chain human insulin analog


<220>
<221>  MISC_FEATURE
<222>  (49)..(49)
<223>  Xaa at position 49 is 4-amino phenylalanine or 4-methoxy 
       phenylalanine

<400>  22

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Gly Ile 
            20                  25                  30          


Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn 
        35                  40                  45              


Xaa Cys Asn 
    50      


<210>  23
<211>  63
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  single-chain human insulin analog


<220>
<221>  MISC_FEATURE
<222>  (61)..(61)
<223>  Xaa at position 61 is 4-amino phenylalanine or 4-methoxy 
       phenylalanine

<400>  23

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Gln Pro 
            20                  25                  30          


Leu Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu Gln Cys 
        35                  40                  45              


Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Xaa Cys Asn 
    50                  55                  60              


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  24

Ala Gly Arg Gly Ser Gly Lys 
1               5           


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  25

Ala Gly Leu Gly Ser Gly Lys 
1               5           


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  26

Ala Gly Met Gly Ser Gly Lys 
1               5           


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  27

Ala Ser Trp Gly Ser Gly Lys 
1               5           


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  28

Thr Gly Leu Gly Ser Gly Gln 
1               5           


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  29

Thr Gly Leu Gly Arg Gly Lys 
1               5           


<210>  30
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  30

Thr Gly Leu Gly Ser Gly Lys 
1               5           


<210>  31
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  31

His Gly Leu Tyr Ser Gly Lys 
1               5           


<210>  32
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  32

Lys Gly Leu Gly Ser Gly Gln 
1               5           


<210>  33
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  33

Val Gly Leu Met Ser Gly Lys 
1               5           


<210>  34
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  34

Val Gly Leu Ser Ser Gly Gln 
1               5           


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  35

Val Gly Leu Tyr Ser Gly Lys 
1               5           


<210>  36
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  36

Val Gly Leu Ser Ser Gly Lys 
1               5           


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  37

Val Gly Met Ser Ser Gly Lys 
1               5           


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  38

Val Trp Ser Ser Ser Gly Lys 
1               5           


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  39

Val Gly Ser Ser Ser Gly Lys 
1               5           


<210>  40
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  40

Val Gly Met Ser Ser Gly Lys 
1               5           


<210>  41
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  41

Thr Gly Leu Gly Ser Gly Arg 
1               5           


<210>  42
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  42

Thr Gly Leu Gly Lys Gly Gln 
1               5           


<210>  43
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  43

Lys Gly Leu Ser Ser Gly Gln 
1               5           


<210>  44
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  44

Val Lys Leu Ser Ser Gly Gln 
1               5           


<210>  45
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  45

Val Lys Leu Ser Ser Gly Gln 
1               5           


<210>  46
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  46

Thr Gly Leu Gly Lys Gly Gln 
1               5           


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  47

Val Gly Leu Ser Lys Gly Gln 
1               5           


<210>  48
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine and threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is threonine or alanine

<400>  48

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Xaa 
            20                  25                  30  


<210>  49
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine and threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is threonine or alanine

<400>  49

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Xaa 
            20                  25                  30  


<210>  50
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine and threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  50

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Lys Thr Arg Arg 
            20                  25                  30          


<210>  51
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine and threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  51

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr Arg Arg 
            20                  25                  30          


<210>  52
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine and threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  52

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


