                         SEQUENCE LISTING

<110>  Carnegie Mellon University
       Bruchez, Marcel
       Ernst, Lauren
       Fitzpatrick, James
       Szent Gyorgyi, Chris
       Schmidt, Brigitte
       Waggoner, Alan
 
<120>  QUENCHED DENDRIMERIC DYES FOR BRIGHT DETECTION

<130>  0018.0006PCT

<150>  US 61/207,929
<151>  2009-02-18

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L5-MG E52D pPNL6 fusion protein

<400>  1

Met Gln Leu Leu Arg Cys Phe Ser Ile Phe Ser Val Ile Ala Ser Val 
1               5                   10                  15      


Leu Ala Gln Glu Leu Thr Thr Ile Cys Glu Gln Ile Pro Ser Pro Thr 
            20                  25                  30          


Leu Glu Ser Thr Pro Tyr Ser Leu Ser Thr Thr Thr Ile Leu Ala Asn 
        35                  40                  45              


Gly Lys Ala Met Gln Gly Val Phe Glu Tyr Tyr Lys Ser Val Thr Phe 
    50                  55                  60                  


Val Ser Asn Cys Gly Ser His Pro Ser Thr Thr Ser Lys Gly Ser Pro 
65                  70                  75                  80  


Ile Asn Thr Gln Tyr Val Phe Lys Asp Asn Ser Ser Thr Ile Glu Gly 
                85                  90                  95      


Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Leu Gln Ala Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Gln 
        115                 120                 125             


Ala Val Val Thr Gln Glu Pro Ser Val Thr Val Ser Pro Gly Gly Thr 
    130                 135                 140                 


Val Ile Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly His 
145                 150                 155                 160 


Tyr Ala Asn Trp Phe Gln Gln Lys Pro Gly Gln Ala Pro Arg Ala Leu 
                165                 170                 175     


Ile Phe Asp Thr Asp Lys Lys Tyr Pro Trp Thr Pro Gly Arg Phe Ser 
            180                 185                 190         


Gly Ser Leu Leu Gly Val Lys Ala Ala Leu Thr Ile Ser Asp Ala Gln 
        195                 200                 205             


Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Leu Leu Ser Asp Val Asp Gly 
    210                 215                 220                 


Tyr Leu Phe Gly Gly Gly Thr Gln Leu Thr Val Leu Ser Gly Ile Leu 
225                 230                 235                 240 


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
                245                 250 


<210>  2
<211>  813
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPNL6 L5-MG E52D nucleotide sequence

<400>  2
aaaaaacccc ggatcgaatt ctacttcata cattttcaat taagatgcag ttacttcgct       60

gtttttcaat attttctgtt attgcttcag ttttagcaca ggaactgaca actatatgcg      120

agcaaatccc ctcaccaact ttagaatcga cgccgtactc tttgtcaacg actactattt      180

tggccaacgg gaaggcaatg caaggagttt ttgaatatta caaatcagta acgtttgtca      240

gtaattgcgg ttctcacccc tcaacaacta gcaaaggcag ccccataaac acacagtatg      300

tttttaagga caatagctcg acgattgaag gtagataccc atacgacgtt ccagactacg      360

ctctgcaggc tagtggtggt ggtggttctg gtggtggtgg ttctggtggt ggtggttctg      420

ctagccaggc tgtggtgact caggagccgt cagtgactgt gtccccagga gggacagtca      480

ttctcacttg tggctccagc actggagctg tcaccagtgg tcattatgcc aactggttcc      540

agcagaaacc tggccaagcc cccagggcac ttatatttga caccgacaag aaatatccct      600

ggacccctgg ccgattctca ggctccctcc ttggggtcaa ggctgccctg accatctcgg      660

atgcgcagcc tgaagatgag gctgagtatt actgtttgct ctccgacgtt gacggttatc      720

tgttcggagg aggcacccag ctgaccgtcc tctccggaat tctagaacaa aagcttattt      780

ctgaagaaga cttgtaatag ctcggcggcc gca                                   813


<210>  3
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  3

Glu Ala Glu Ala Tyr Gln Ala Val Val Thr Gln Glu Pro Ser Val Thr 
1               5                   10                  15      


Val Ser Pro Gly Gly Thr Val Ile Leu Thr Cys Gly Ser Ser Thr Gly 
            20                  25                  30          


Ala Val Thr Ser Gly His Tyr Ala Asn Trp Phe Gln Gln Lys Pro Gly 
        35                  40                  45              


Gln Ala Pro Arg Ala Leu Ile Phe Glu Thr Asp Lys Lys Tyr Pro Trp 
    50                  55                  60                  


Thr Pro Gly Arg Phe Ser Gly Ser Leu Leu Gly Val Lys Ala Ala Leu 
65                  70                  75                  80  


Thr Ile Ser Asp Ala Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Leu 
                85                  90                  95      


Leu Ser Asp Val Asp Gly Tyr Leu Phe Gly Gly Gly Thr Gln Leu Thr 
            100                 105                 110         


Val Leu Ser Thr Gly His His His His His His 
        115                 120             


<210>  4
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv

<400>  4

Glu Ala Glu Ala Tyr Gln Ala Val Val Thr Gln Glu Pro Ser Val Thr 
1               5                   10                  15      


Val Ser Pro Gly Gly Thr Val Ile Leu Thr Cys Gly Ser Ser Thr Gly 
            20                  25                  30          


Ala Val Thr Ser Gly His Tyr Ala Asn Trp Phe Gln Gln Lys Pro Gly 
        35                  40                  45              


Gln Ala Pro Arg Ala Leu Ile Phe Asp Thr Asp Lys Lys Tyr Pro Trp 
    50                  55                  60                  


Thr Pro Gly Arg Phe Ser Gly Ser Leu Leu Gly Val Lys Ala Ala Leu 
65                  70                  75                  80  


Thr Ile Ser Asp Ala Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Leu 
                85                  90                  95      


Leu Ser Asp Val Asp Gly Tyr Leu Phe Gly Gly Gly Thr Gln Leu Thr 
            100                 105                 110         


Val Leu Ser Thr Gly His His His His His His 
        115                 120             


<210>  5
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv

<400>  5

Glu Ala Glu Ala Tyr Gln Ala Val Val Thr Gln Glu Pro Ser Val Thr 
1               5                   10                  15      


Val Ser Pro Gly Gly Thr Val Ile Leu Thr Cys Gly Ser Ser Thr Gly 
            20                  25                  30          


Ala Val Thr Ser Gly His Tyr Ala Asn Trp Phe Gln Gln Lys Pro Gly 
        35                  40                  45              


Gln Ala Pro Arg Ala Leu Ile Phe Glu Thr Asp Lys Lys Tyr Pro Trp 
    50                  55                  60                  


Thr Pro Gly Arg Phe Ser Gly Ser Leu Leu Gly Val Lys Ala Ala Leu 
65                  70                  75                  80  


Thr Ile Ser Asp Ala Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Ser 
                85                  90                  95      


Leu Ser Asp Val Asp Gly Tyr Leu Phe Gly Gly Gly Thr Gln Leu Thr 
            100                 105                 110         


Val Leu Ser Thr Gly His His His His His His 
        115                 120             


<210>  6
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv

<400>  6

Glu Ala Glu Ala Tyr Gln Ala Val Val Thr Gln Glu Pro Ser Val Thr 
1               5                   10                  15      


Val Ser Pro Gly Gly Thr Val Ile Leu Thr Cys Gly Ser Gly Thr Gly 
            20                  25                  30          


Ala Val Thr Ser Gly His Tyr Ala Asn Trp Phe Gln Gln Lys Pro Gly 
        35                  40                  45              


Gln Ala Pro Arg Ala Leu Ile Phe Asp Thr Asp Lys Lys Tyr Pro Trp 
    50                  55                  60                  


Thr Pro Gly Arg Phe Ser Gly Ser Leu Leu Gly Val Lys Ala Ala Leu 
65                  70                  75                  80  


Thr Ile Ser Asp Ala Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Ser 
                85                  90                  95      


Leu Ser Asp Val Asp Gly Tyr Leu Phe Gly Gly Gly Thr Gln Leu Thr 
            100                 105                 110         


Val Leu Ser Thr Gly His His His His His His 
        115                 120             


<210>  7
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv

<400>  7

Gln Val Gln Leu Val Glu Ser Glu Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Glu Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Arg Ile Asp Gly Asp Gly Ser Ser Thr Asn Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ala Arg Tyr Phe Gly Ser Val Ser Pro Tyr Gly Met Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ile Leu Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
    130                 135                 140                 


Ile Arg Val Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp 
145                 150                 155                 160 


Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Gly Ile Ala Thr Trp Leu 
                165                 170                 175     


Gly Trp Tyr Gln Gln Lys Pro Gly Lys Pro Pro Gln Leu Leu Ile Tyr 
            180                 185                 190         


Ser Ala Ser Thr Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
    210                 215                 220                 


Asp Val Ala Thr Tyr Tyr Cys Gln Glu Gly Ser Thr Phe Pro Leu Thr 
225                 230                 235                 240 


Phe Gly Gly Gly Thr Lys Val Asp Ile Lys Ser 
                245                 250     


<210>  8
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv

<400>  8

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


His Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro Glu 
        35                  40                  45              


Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro Ala 
    50                  55                  60                  


Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln Phe 
65                  70                  75                  80  


Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly Pro Ile 
            100                 105                 110         


Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu Val Thr 
        115                 120                 125             


Val Ser 
    130 


<210>  9
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv

<400>  9

Ser Tyr Glu Leu Thr Gln Pro Pro Ser Val Ser Val Ser Pro Gly Gln 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Ser Gly Asp Ala Leu Pro Lys Gln Tyr Thr 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Ala Gly Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Lys Asp Thr Glu Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Thr 
    50                  55                  60                  


Ser Ser Gly Thr Thr Val Thr Leu Thr Ile Ser Gly Val Gln Ala Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Ala Asp Ser Ser Gly Ser Tyr 
                85                  90                  95      


Val Phe Phe Gly Gly Gly Thr Lys Val Thr Val Leu Ser 
            100                 105                 


