                         SEQUENCE LISTING

<110>  UWS Ventures Ltd.
 
<120>  Yeast for fructan utilisation

<130>  55003WO

<150>  GB 0903224,4
<151>  2009-02-26

<160>  4     

<170>  PatentIn version 3.3

<210>  1
<211>  2249
<212>  DNA
<213>  Bacillus subtilis

<400>  1
gcggccgcga attcagatct atgaggtttc cctcaatctt taccgccgtt ttgttcgctg     60

catcttcagc attagctgca ccagtcaata caaccacaga ggatgaaaca gctcaaatac    120

cagcagaagc cgttattggt tatttggact tagaaggaga ttttgacgtt gcagtattac    180

cttttagtaa ctcaactaat aacggtttgt tatttatcaa taccaccata gcttcaattg    240

cagccaaaga agaaggcgtt tctttggaaa agagagaagc agaagcagca gattcatctt    300

actacgatga agactatagg ccccagtacc actttacccc tgaagccaat tggatgaatg    360

atccaaacgg tatggtgtat tacgctggag aataccattt gttctatcaa taccatcctt    420

atggtttaca gtggggcccc atgcactggg gccacgctgt gagtaaagac ttggtaacat    480

gggagcattt acctgtggca ttgtaccctg atgaaaaagg taccattttc tcaggttctg    540

cagttgtgga caaaaacaat acttctggtt ttcagactgg caaggaaaaa cctttagtag    600

ccatatatac acaagataga gaaggtcacc aagtccaatc aattgcttac tctaatgaca    660

aaggaagaac ctggacaaaa tacgcaggaa atccagttat accaaatccc ggaaagaaag    720

attttaggga ccctaaagta ttctggtatg agaaagaaaa gaaatgggtc atggtcttgg    780

ctgctggtga tagaatcttg atttatacat caaaaaattt gaaacaatgg acttacgctt    840

ctgagttcgg ccaagaccaa ggatcccacg gcggcgtttg ggaatgtcca gatttatttg    900

aattgcctgt tgacggaaat ccaaatcaga aaaagtgggt catgcaagtc tcagtgggaa    960

atggcgctgt aagtggtggt tcaggtatgc aatacttcgt gggtgatttt gatggcactc   1020

atttcaaaaa tgaaaatcct ccaaataaag tgttatggac agattatggt agagattttt   1080

atgccgcagt cagttggtcc gacattcctt ctactgacag taggagattg tggttgggat   1140

ggatgtcaaa ctggcagtac gccaacgatg taccaacctc cccatggaga agtgcaacta   1200

gtatcccaag agaattgaaa ttgaaagcat ttacagaagg agtcagagtg gtacagaccc   1260

ctgttaagga attagagact attagaggta ctagtaaaaa gtggaagaac ttaactatat   1320

ctcccgcctc ccataacgtt ttggctggtc aatcaggtga tgcttatgaa atcaacgccg   1380

aatttaaagt ttcccctggt tcagctgctg aatttggttt caaggttaga accggtgaga   1440

accaattcac aaaagttggt tatgatagaa gaaatgcaaa gttgtttgtt gacaggtctg   1500

agtctggtaa tgatacattt aatcctgctt tcaatacagg taaggagact gcaccattaa   1560

aaccagttaa tggtaaagtc aaattgagaa tttttgtaga cagatcatcc gttgaagttt   1620

ttggaaatga tggcaaacaa gtcatcaccg atatcatttt gcctgataga tcctctaagg   1680

gtttggagtt gtatgctgca aacggtggtg ttaaggtaaa gtctttgact atacatccat   1740

taaagaaggt ttggggtacc accccattca tgagtaacat gactggttgg actactgtaa   1800

atggcacatg ggcagacact atcgaaggta aacagggcag aagtgatgga gacagtttca   1860

ttttgtcctc tgcttctggt tctgacttta catatgagtc tgatattacc ataaaggatg   1920

gtaacggtag aggagctgga gctttaatgt tcagaagtga taaagatgct aaaaatggct   1980

atttagctaa cgtggacgct aagcatgatt tagtgaagtt tttcaaattt gagaacggag   2040

ccgcttctgt tattgcagag tataaaaccc ctatagatgt caacaagaag tatcatttaa   2100

aaaccgaagc cgaaggtgat agattcaaga tttacttgga tgacagattg gtcattgatg   2160

ctcatgatag tgtattctcc gaaggccaat ttggcttgaa tgtttgggat gccacagccg   2220

tttttcaaaa cgtaacaaag gagtcctaa                                     2249


<210>  2
<211>  742
<212>  PRT
<213>  Bacillus subtilis

<400>  2

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg Glu Ala Glu Ala Ala Asp Ser Ser Tyr Tyr Asp 
                85                  90                  95      


Glu Asp Tyr Arg Pro Gln Tyr His Phe Thr Pro Glu Ala Asn Trp Met 
            100                 105                 110         


Asn Asp Pro Asn Gly Met Val Tyr Tyr Ala Gly Glu Tyr His Leu Phe 
        115                 120                 125             


Tyr Gln Tyr His Pro Tyr Gly Leu Gln Trp Gly Pro Met His Trp Gly 
    130                 135                 140                 


His Ala Val Ser Lys Asp Leu Val Thr Trp Glu His Leu Pro Val Ala 
145                 150                 155                 160 


Leu Tyr Pro Asp Glu Lys Gly Thr Ile Phe Ser Gly Ser Ala Val Val 
                165                 170                 175     


Asp Lys Asn Asn Thr Ser Gly Phe Gln Thr Gly Lys Glu Lys Pro Leu 
            180                 185                 190         


Val Ala Ile Tyr Thr Gln Asp Arg Glu Gly His Gln Val Gln Ser Ile 
        195                 200                 205             


Ala Tyr Ser Asn Asp Lys Gly Arg Thr Trp Thr Lys Tyr Ala Gly Asn 
    210                 215                 220                 


Pro Val Ile Pro Asn Pro Gly Lys Lys Asp Phe Arg Asp Pro Lys Val 
225                 230                 235                 240 


Phe Trp Tyr Glu Lys Glu Lys Lys Trp Val Met Val Leu Ala Ala Gly 
                245                 250                 255     


Asp Arg Ile Leu Ile Tyr Thr Ser Lys Asn Leu Lys Gln Trp Thr Tyr 
            260                 265                 270         


Ala Ser Glu Phe Gly Gln Asp Gln Gly Ser His Gly Gly Val Trp Glu 
        275                 280                 285             


Cys Pro Asp Leu Phe Glu Leu Pro Val Asp Gly Asn Pro Asn Gln Lys 
    290                 295                 300                 


Lys Trp Val Met Gln Val Ser Val Gly Asn Gly Ala Val Ser Gly Gly 
305                 310                 315                 320 


Ser Gly Met Gln Tyr Phe Val Gly Asp Phe Asp Gly Thr His Phe Lys 
                325                 330                 335     


Asn Glu Asn Pro Pro Asn Lys Val Leu Trp Thr Asp Tyr Gly Arg Asp 
            340                 345                 350         


Phe Tyr Ala Ala Val Ser Trp Ser Asp Ile Pro Ser Thr Asp Ser Arg 
        355                 360                 365             


Arg Leu Trp Leu Gly Trp Met Ser Asn Trp Gln Tyr Ala Asn Asp Val 
    370                 375                 380                 


Pro Thr Ser Pro Trp Arg Ser Ala Thr Ser Ile Pro Arg Glu Leu Lys 
385                 390                 395                 400 


Leu Lys Ala Phe Thr Glu Gly Val Arg Val Val Gln Thr Pro Val Lys 
                405                 410                 415     


Glu Leu Glu Thr Ile Arg Gly Thr Ser Lys Lys Trp Lys Asn Leu Thr 
            420                 425                 430         


Ile Ser Pro Ala Ser His Asn Val Leu Ala Gly Gln Ser Gly Asp Ala 
        435                 440                 445             


Tyr Glu Ile Asn Ala Glu Phe Lys Val Ser Pro Gly Ser Ala Ala Glu 
    450                 455                 460                 


Phe Gly Phe Lys Val Arg Thr Gly Glu Asn Gln Phe Thr Lys Val Gly 
465                 470                 475                 480 


Tyr Asp Arg Arg Asn Ala Lys Leu Phe Val Asp Arg Ser Glu Ser Gly 
                485                 490                 495     


Asn Asp Thr Phe Asn Pro Ala Phe Asn Thr Gly Lys Glu Thr Ala Pro 
            500                 505                 510         


Leu Lys Pro Val Asn Gly Lys Val Lys Leu Arg Ile Phe Val Asp Arg 
        515                 520                 525             


Ser Ser Val Glu Val Phe Gly Asn Asp Gly Lys Gln Val Ile Thr Asp 
    530                 535                 540                 


Ile Ile Leu Pro Asp Arg Ser Ser Lys Gly Leu Glu Leu Tyr Ala Ala 
545                 550                 555                 560 


Asn Gly Gly Val Lys Val Lys Ser Leu Thr Ile His Pro Leu Lys Lys 
                565                 570                 575     


Val Trp Gly Thr Thr Pro Phe Met Ser Asn Met Thr Gly Trp Thr Thr 
            580                 585                 590         


Val Asn Gly Thr Trp Ala Asp Thr Ile Glu Gly Lys Gln Gly Arg Ser 
        595                 600                 605             


Asp Gly Asp Ser Phe Ile Leu Ser Ser Ala Ser Gly Ser Asp Phe Thr 
    610                 615                 620                 


Tyr Glu Ser Asp Ile Thr Ile Lys Asp Gly Asn Gly Arg Gly Ala Gly 
625                 630                 635                 640 


Ala Leu Met Phe Arg Ser Asp Lys Asp Ala Lys Asn Gly Tyr Leu Ala 
                645                 650                 655     


Asn Val Asp Ala Lys His Asp Leu Val Lys Phe Phe Lys Phe Glu Asn 
            660                 665                 670         


Gly Ala Ala Ser Val Ile Ala Glu Tyr Lys Thr Pro Ile Asp Val Asn 
        675                 680                 685             


Lys Lys Tyr His Leu Lys Thr Glu Ala Glu Gly Asp Arg Phe Lys Ile 
    690                 695                 700                 


Tyr Leu Asp Asp Arg Leu Val Ile Asp Ala His Asp Ser Val Phe Ser 
705                 710                 715                 720 


Glu Gly Gln Phe Gly Leu Asn Val Trp Asp Ala Thr Ala Val Phe Gln 
                725                 730                 735     


Asn Val Thr Lys Glu Ser 
            740         


<210>  3
<211>  2428
<212>  DNA
<213>  Lactobacillus paracasei

<400>  3
gcggccgcag atctaaaatg agattcccct ccatatttac agctgtcctt ttcgcagcga     60

gttctgcttt ggcagcgccg gtcaacacca caacagaaga cgagaccgct cagatccccg    120

ccgaagcagt cataggctat ttggatttgg agggtgactt tgacgttgcc gttttgccgt    180

tctccaactc tacaaacaat ggcctattgt tcatcaatac taccatagct tctatcgcag    240

caaaagaaga gggtgtttca ctagaaaaga gggaagcaga ggcggctaca tcggcaagtt    300

ctacacagat tagtcagaca aacactggtt cgcagcccaa caacgaaacc accggggaaa    360

cagcccaatc ttcggtaaat tccactgcta ccgcaagttc cagttcagtt gctgacttgc    420

catctagctc agatagcaaa agttcaattg gttccactat ttctcagcca acagttgaca    480

aaaaggagac ttccaaatcc gatacggctg acaatgatct tacaaagagc gttacaacca    540

gtgattctga caaagcacta cccacttcaa agaccacact gcctacgtca aacgaacaag    600

tgcaatcgtc agttggtcag agtcaaaccg atcaatccgc gtcctcggct acgatcgcaa    660

cgaatgcagt cacttcagat gtttcccaaa acgaccaata caacgaacca tatagaaatc    720

aatatcatta tagctcttct caaaactgga taaacgaccc taacggtctt ttctacgata    780

gtaaaacggg cttatataac ctatattatc agtacaatcc agaaggtaat caatggggca    840

acatgtcatg gggtcacgct gtgtcaaaag acttaataaa ctggactcaa gaagatgtag    900

caatcccaat gttgcagaat caaggatggg aggactttac ttacacgaac accacaggtt    960

ctctaaaaga caaaggagaa gttagatatg tgggtgtgcc tacaactaat tggggtgatg   1020

ctgatggtaa gaaagctatc ttttctggtt ctattgtcgt tgatacaaat aacgtatccg   1080

gtctagggaa ggatgctata ttggcatttt ataccgccga ctaccaaatt gcgactagga   1140

agaatgatgg tgcagaagat ggttggggca cttggatcgg tttaaccgaa atacaagaac   1200

aacatctggc ttattcgtta gacggaggta aaacatttat tcaatatagt aaagacggta   1260

atgctgctaa tcctcaagct atcattccta caagtatgaa ccagggaggt gacgcggcta   1320

atttcagaga tccgtcagtt gtttacgacg ccgttaataa acaatattac ttaactgtgg   1380

tgtctggcca gcaagccctt atatacaaga gcagcaattt gctggattgg acgtatgcat   1440

cgaaaataga gagggaaaat gatgttggta atggagtctg ggaatgccct agcttagtcc   1500

cgatgaaggt agcaggtact aacgaaacta aatgggtatt ttgtataagt gtccagcaag   1560

gtgctcatgc tacaggatca ggtatgcagt actacgtggg taatatgacc gccgatggga   1620

cctgggtacc agaatccagc aaaactttac aaaatccaat gaccatggat tcaggagagg   1680

atttttacgc agggatccca ttctctaata tgccagacgg aagaactgta atgctggcct   1740

ggcaatcgaa ttggagttac gtcgatgaag caaaaacatc cccatggtct gggaacatga   1800

cattaccacg tgagctgtcg ttgaagaaaa acgccgatac tacagatggc tatcttttaa   1860

ctaatacagt ggtcaaggaa attgcgaaca atgaagaagc caatgttata aataaggctg   1920

aatctaattt tactgtttct aggagtgatg aacaagttca atatgaaggg aagcaataca   1980

aaatttcagc tacattttct tgggacgaag ccgacaagcc taaatccgtt ggatttaagt   2040

tacgtgtctc tgatgatcaa aaatacgata tgattgttgg atatgattta actactggat   2100

tgttgtatgt gcaaagatta aatactggcg agcctaatat gggcgctcct cgtgataaga   2160

tgaatgccac tgtaaacgcc gatggttcaa ttacaatcac tgtttacgta gatgagacgt   2220

ctattgaagc attcgccaat gatggagaaa aatctattac ccaaaacttc tttatgagac   2280

cagagaatat tggagatcag gctactacgg gcgtatatgt atattcaaac gatggcacta   2340

ctaaaattag cgatttaacg attaatccaa ttacctcaat ttggaattca actggtcaat   2400

tgacggaaaa gtagagatct gcggccgc                                      2428


<210>  4
<211>  798
<212>  PRT
<213>  Lactobacillus paracasei

<400>  4

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg Glu Ala Glu Ala Ala Thr Ser Ala Ser Ser Thr 
                85                  90                  95      


Gln Ile Ser Gln Thr Asn Thr Gly Ser Gln Pro Asn Asn Glu Thr Thr 
            100                 105                 110         


Gly Glu Thr Ala Gln Ser Ser Val Asn Ser Thr Ala Thr Ala Ser Ser 
        115                 120                 125             


Ser Ser Val Ala Asp Leu Pro Ser Ser Ser Asp Ser Lys Ser Ser Ile 
    130                 135                 140                 


Gly Ser Thr Ile Ser Gln Pro Thr Val Asp Lys Lys Glu Thr Ser Lys 
145                 150                 155                 160 


Ser Asp Thr Ala Asp Asn Asp Leu Thr Lys Ser Val Thr Thr Ser Asp 
                165                 170                 175     


Ser Asp Lys Ala Leu Pro Thr Ser Lys Thr Thr Leu Pro Thr Ser Asn 
            180                 185                 190         


Glu Gln Val Gln Ser Ser Val Gly Gln Ser Gln Thr Asp Gln Ser Ala 
        195                 200                 205             


Ser Ser Ala Thr Ile Ala Thr Asn Ala Val Thr Ser Asp Val Ser Gln 
    210                 215                 220                 


Asn Asp Gln Tyr Asn Glu Pro Tyr Arg Asn Gln Tyr His Tyr Ser Ser 
225                 230                 235                 240 


Ser Gln Asn Trp Ile Asn Asp Pro Asn Gly Leu Phe Tyr Asp Ser Lys 
                245                 250                 255     


Thr Gly Leu Tyr Asn Leu Tyr Tyr Gln Tyr Asn Pro Glu Gly Asn Gln 
            260                 265                 270         


Trp Gly Asn Met Ser Trp Gly His Ala Val Ser Lys Asp Leu Ile Asn 
        275                 280                 285             


Trp Thr Gln Glu Asp Val Ala Ile Pro Met Leu Gln Asn Gln Gly Trp 
    290                 295                 300                 


Glu Asp Phe Thr Tyr Thr Asn Thr Thr Gly Ser Leu Lys Asp Lys Gly 
305                 310                 315                 320 


Glu Val Arg Tyr Val Gly Val Pro Thr Thr Asn Trp Gly Asp Ala Asp 
                325                 330                 335     


Gly Lys Lys Ala Ile Phe Ser Gly Ser Ile Val Val Asp Thr Asn Asn 
            340                 345                 350         


Val Ser Gly Leu Gly Lys Asp Ala Ile Leu Ala Phe Tyr Thr Ala Asp 
        355                 360                 365             


Tyr Gln Ile Ala Thr Arg Lys Asn Asp Gly Ala Glu Asp Gly Trp Gly 
    370                 375                 380                 


Thr Trp Ile Gly Leu Thr Glu Ile Gln Glu Gln His Leu Ala Tyr Ser 
385                 390                 395                 400 


Leu Asp Gly Gly Lys Thr Phe Ile Gln Tyr Ser Lys Asp Gly Asn Ala 
                405                 410                 415     


Ala Asn Pro Gln Ala Ile Ile Pro Thr Ser Met Asn Gln Gly Gly Asp 
            420                 425                 430         


Ala Ala Asn Phe Arg Asp Pro Ser Val Val Tyr Asp Ala Val Asn Lys 
        435                 440                 445             


Gln Tyr Tyr Leu Thr Val Val Ser Gly Gln Gln Ala Leu Ile Tyr Lys 
    450                 455                 460                 


Ser Ser Asn Leu Leu Asp Trp Thr Tyr Ala Ser Lys Ile Glu Arg Glu 
465                 470                 475                 480 


Asn Asp Val Gly Asn Gly Val Trp Glu Cys Pro Ser Leu Val Pro Met 
                485                 490                 495     


Lys Val Ala Gly Thr Asn Glu Thr Lys Trp Val Phe Cys Ile Ser Val 
            500                 505                 510         


Gln Gln Gly Ala His Ala Thr Gly Ser Gly Met Gln Tyr Tyr Val Gly 
        515                 520                 525             


Asn Met Thr Ala Asp Gly Thr Trp Val Pro Glu Ser Ser Lys Thr Leu 
    530                 535                 540                 


Gln Asn Pro Met Thr Met Asp Ser Gly Glu Asp Phe Tyr Ala Gly Ile 
545                 550                 555                 560 


Pro Phe Ser Asn Met Pro Asp Gly Arg Thr Val Met Leu Ala Trp Gln 
                565                 570                 575     


Ser Asn Trp Ser Tyr Val Asp Glu Ala Lys Thr Ser Pro Trp Ser Gly 
            580                 585                 590         


Asn Met Thr Leu Pro Arg Glu Leu Ser Leu Lys Lys Asn Ala Asp Thr 
        595                 600                 605             


Thr Asp Gly Tyr Leu Leu Thr Asn Thr Val Val Lys Glu Ile Ala Asn 
    610                 615                 620                 


Asn Glu Glu Ala Asn Val Ile Asn Lys Ala Glu Ser Asn Phe Thr Val 
625                 630                 635                 640 


Ser Arg Ser Asp Glu Gln Val Gln Tyr Glu Gly Lys Gln Tyr Lys Ile 
                645                 650                 655     


Ser Ala Thr Phe Ser Trp Asp Glu Ala Asp Lys Pro Lys Ser Val Gly 
            660                 665                 670         


Phe Lys Leu Arg Val Ser Asp Asp Gln Lys Tyr Asp Met Ile Val Gly 
        675                 680                 685             


Tyr Asp Leu Thr Thr Gly Leu Leu Tyr Val Gln Arg Leu Asn Thr Gly 
    690                 695                 700                 


Glu Pro Asn Met Gly Ala Pro Arg Asp Lys Met Asn Ala Thr Val Asn 
705                 710                 715                 720 


Ala Asp Gly Ser Ile Thr Ile Thr Val Tyr Val Asp Glu Thr Ser Ile 
                725                 730                 735     


Glu Ala Phe Ala Asn Asp Gly Glu Lys Ser Ile Thr Gln Asn Phe Phe 
            740                 745                 750         


Met Arg Pro Glu Asn Ile Gly Asp Gln Ala Thr Thr Gly Val Tyr Val 
        755                 760                 765             


Tyr Ser Asn Asp Gly Thr Thr Lys Ile Ser Asp Leu Thr Ile Asn Pro 
    770                 775                 780                 


Ile Thr Ser Ile Trp Asn Ser Thr Gly Gln Leu Thr Glu Lys 
785                 790                 795             


