                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
       DSM Fine Chemicals Austria Nfg GmbH & Co KG
 
<120>  Improved pig liver esterases

<130>  27193-WO-PCT

<150>  EP09158759.2
<151>  2009-04-24

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  6407
<212>  DNA
<213>  Unknown

<220>
<223>  Plasmid


<220>
<221>  exon
<222>  (4754)..(6397)
<223>  APLE (alternative Pig Liver Esterase]

<220>
<221>  CDS
<222>  (4754)..(6397)

<400>  1
ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga       60

agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc      120

atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg      180

agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt      240

tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc      300

ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac      360

tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca      420

aactcttttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gacgaataaa      480

tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg      540

ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca      600

actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt      660

caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat      720

cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata      780

accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca      840

agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc      900

gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg      960

ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc     1020

ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt     1080

tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca     1140

ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg     1200

gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg     1260

ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg     1320

agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg     1380

ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac     1440

ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc     1500

ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagtta     1560

gatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta     1620

gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     1680

acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     1740

tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag     1800

ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     1860

atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     1920

agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     1980

cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa     2040

agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     2100

acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     2160

gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     2220

ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt     2280

gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt     2340

gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag     2400

gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac     2460

cgcacgaacg ccagcaagac gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact     2520

tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct     2580

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg     2640

tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag     2700

agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgctgg     2760

tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga     2820

tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct     2880

gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt     2940

gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat     3000

tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg     3060

ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc     3120

gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa     3180

gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca     3240

gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt     3300

tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat     3360

cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg     3420

tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa     3480

tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc     3540

tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat     3600

cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc     3660

atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg ccgcttcgcc     3720

ttcgcgcgcg aattgcaagc tgatccgggc ttatcgactg cacggtgcac caatgcttct     3780

ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa     3840

ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac     3900

ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg     3960

tggaattgtg agcggataac aatttcacac aggaaacaga attcgagctc ggtacccggg     4020

gatcctttaa ctttaagaag gagatataat ggatgacgcg gcaattcaac aaacgttagc     4080

caaaatgggc atcaaaagca gcgatattca gcccgcgcct gtagctggca tgaagacagt     4140

tctgactaac agcggcgtgt tgtacatcac cgatgatggt aaacatatca ttcaggggcc     4200

aatgtatgac gttagtggca cggctccggt caatgtcacc aataagatgc tgttaaagca     4260

gttgaatgcg cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat     4320

caccgtgttt actgatatta cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga     4380

ctacaacgcg ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag     4440

cgatgcagag aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga     4500

tgatgtgatg gcaggtaaaa gcgtcgcacc agccagttgc gacgtggata ttgccgacca     4560

ttacgcactt ggcgtccagc ttggcgttag cggtactccg gcagttgtgc tgagcaatgg     4620

cacacttgtt ccgggttacc agccgccgaa agagatgaaa gaattcctcg acgaacacca     4680

aaaaatgacc agcggtaaat aaggatcctc tagaaataat tttgtttaac tttaagaagg     4740

agatatacat atg gga caa cca gct tcg ccg cct gtc gtt gat acc gct        4789
               Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala            
               1               5                   10                     

caa gga cga gtc ttg ggt aag tac gtc tct tta gag gga ttg gca caa       4837
Gln Gly Arg Val Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln           
        15                  20                  25                        

ccg gtt gct gtc ttc ttg gga gtc cct ttt gct aag cca cct ctt gga       4885
Pro Val Ala Val Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly           
    30                  35                  40                            

tct ttg agg ttt gcc ccg ccg caa cca gca gag cca tgg tct ttc gtt       4933
Ser Leu Arg Phe Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val           
45                  50                  55                  60            

aag aac act act tcc tac cct cca atg tgt tgt caa gaa cca atc gga       4981
Lys Asn Thr Thr Ser Tyr Pro Pro Met Cys Cys Gln Glu Pro Ile Gly           
                65                  70                  75                

gga caa atg ctt tca gac cta ttc act aac aga aag gaa agg ctt atc       5029
Gly Gln Met Leu Ser Asp Leu Phe Thr Asn Arg Lys Glu Arg Leu Ile           
            80                  85                  90                    

ccg gag ttc tct gag gat tgc ctt tac cta aat att tac act cct gcc       5077
Pro Glu Phe Ser Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala           
        95                  100                 105                       

gat ttg aca aag agg ggt agg ttg ccg gtt atg gtt tgg att cat gga       5125
Asp Leu Thr Lys Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly           
    110                 115                 120                           

gga ggt ttg gtt gtt ggc gga gca tcc act tat gac gga ttg gct ctt       5173
Gly Gly Leu Val Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu           
125                 130                 135                 140           

gcc gcg cac gag aac gtt gtt gtt gtt gct att caa tac cgt ttg ggt       5221
Ala Ala His Glu Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly           
                145                 150                 155               

att tgg gga ttt ttc tcc aca gga gat gag cat tcc cgt gga aac tgg       5269
Ile Trp Gly Phe Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp           
            160                 165                 170                   

ggc cat tta gat caa gtt gct gca ttg cat tgg gtc caa gaa aac att       5317
Gly His Leu Asp Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile           
        175                 180                 185                       

gct aac ttc gga ggt gat cca ggt tct gtt act att ttc gga gaa tca       5365
Ala Asn Phe Gly Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser           
    190                 195                 200                           

gca ggc gga gag agt gtc tct gta ttg gtt tta tca cca tta gct aag       5413
Ala Gly Gly Glu Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys           
205                 210                 215                 220           

aac ctt ttt cat cgt gct att tcc gaa agt ggt gtt gct ttt acc gcc       5461
Asn Leu Phe His Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala           
                225                 230                 235               

ggt ttg gtc agg aag gat atg aag gcc gca gcc aag cag atc gct gtc       5509
Gly Leu Val Arg Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val           
            240                 245                 250                   

ctt gca gga tgc aaa act act act tcg gca gtc ttc gtg cat tgt ttg       5557
Leu Ala Gly Cys Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu           
        255                 260                 265                       

cgt caa aag tcg gaa gat gaa ctt tta gac ctc acg ttg aag atg aaa       5605
Arg Gln Lys Ser Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys           
    270                 275                 280                           

ttc ttt gcc ctt gac tta cac gga gat cca agg gaa tct cac cct ttt       5653
Phe Phe Ala Leu Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe           
285                 290                 295                 300           

ttg acc act gtt gtt gac gga gtt ttg ttg cct aag atg cct gag gaa       5701
Leu Thr Thr Val Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu           
                305                 310                 315               

atc ttg gcc gag aag gac ttt aac acc gtc cca tac att gtt gga att       5749
Ile Leu Ala Glu Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile           
            320                 325                 330                   

aac aag cag gag ttc gga tgg ctt ttg cca acg atg atg gga ttt cct       5797
Asn Lys Gln Glu Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro           
        335                 340                 345                       

ctt tcc gag gga aag ttg gat caa aag acg gct acg tca ctt ttg tgg       5845
Leu Ser Glu Gly Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp           
    350                 355                 360                           

aag tcc tac cca att gcc aac att cct gaa gag ttg acc cca gtt gct       5893
Lys Ser Tyr Pro Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala           
365                 370                 375                 380           

acc gat aag tat tta gga gga aca gat gat cct gtc aaa aag aaa gat       5941
Thr Asp Lys Tyr Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp           
                385                 390                 395               

ttg ttt ttg gat ctg atg gga gac gtt gtt ttc ggc gtc cca tca gtt       5989
Leu Phe Leu Asp Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val           
            400                 405                 410                   

acg gtt gct cgt cag cat agg gac gca gga gct cca act tac atg tat       6037
Thr Val Ala Arg Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr           
        415                 420                 425                       

gag ttc caa tat cgt cca tct ttt tca tcg gat aag aaa cct aag acg       6085
Glu Phe Gln Tyr Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr           
    430                 435                 440                           

gtt att gga gat cat gga gac gaa att ttt tcc gtc ttc ggc ttc cca       6133
Val Ile Gly Asp His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro           
445                 450                 455                 460           

ttg ctc aaa ggt gac gct cca gag gaa gaa gtc agt ctt tct aag acg       6181
Leu Leu Lys Gly Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr           
                465                 470                 475               

gtt atg aaa ttt tgg gct aac ttc gcc cgt agt gga aac cct aat gga       6229
Val Met Lys Phe Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly           
            480                 485                 490                   

gaa gga ttg cct cac tgg ccg atg tac gat caa gag gag gga tac ctt       6277
Glu Gly Leu Pro His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu           
        495                 500                 505                       

caa att ggt gtc aac act caa gca gct aag agg ttg aaa ggc gag gag       6325
Gln Ile Gly Val Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu           
    510                 515                 520                           

gtt gct ttt tgg aac gac ctg ttg tcc aag gaa gca gca aag aag cca       6373
Val Ala Phe Trp Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro           
525                 530                 535                 540           

cct aag ata aag cac gcc gaa ttg taataagctt                            6407
Pro Lys Ile Lys His Ala Glu Leu                                           
                545                                                       


<210>  2
<211>  548
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  2

Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val 
1               5                   10                  15      


Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val 
            20                  25                  30          


Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe 
        35                  40                  45              


Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr 
    50                  55                  60                  


Ser Tyr Pro Pro Met Cys Cys Gln Glu Pro Ile Gly Gly Gln Met Leu 
65                  70                  75                  80  


Ser Asp Leu Phe Thr Asn Arg Lys Glu Arg Leu Ile Pro Glu Phe Ser 
                85                  90                  95      


Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys 
            100                 105                 110         


Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val 
        115                 120                 125             


Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala His Glu 
    130                 135                 140                 


Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe 
145                 150                 155                 160 


Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp 
                165                 170                 175     


Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly 
            180                 185                 190         


Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu 
        195                 200                 205             


Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His 
    210                 215                 220                 


Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu Val Arg 
225                 230                 235                 240 


Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys 
                245                 250                 255     


Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser 
            260                 265                 270         


Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Phe Ala Leu 
        275                 280                 285             


Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr Thr Val 
    290                 295                 300                 


Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu 
305                 310                 315                 320 


Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu 
                325                 330                 335     


Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly 
            340                 345                 350         


Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Tyr Pro 
        355                 360                 365             


Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr 
    370                 375                 380                 


Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp 
385                 390                 395                 400 


Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg 
                405                 410                 415     


Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr 
            420                 425                 430         


Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp 
        435                 440                 445             


His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu Lys Gly 
    450                 455                 460                 


Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe 
465                 470                 475                 480 


Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro 
                485                 490                 495     


His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val 
            500                 505                 510         


Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp 
        515                 520                 525             


Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys 
    530                 535                 540                 


His Ala Glu Leu 
545             


<210>  3
<211>  1660
<212>  DNA
<213>  Unknown

<220>
<223>  Synthetic gene


<220>
<221>  exon
<222>  (7)..(1650)
<223>  gamma PLE

<220>
<221>  CDS
<222>  (7)..(1650)
<223>  gamma PLE

<400>  3
catatg gga caa cca gct tcg ccg cct gtc gtt gat acc gct caa gga          48
       Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly            
       1               5                   10                             

cga gtc ttg ggt aag tac gtc tct tta gag gga ttg gca caa ccg gtt         96
Arg Val Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val           
15                  20                  25                  30            

gct gtc ttc ttg gga gtc cct ttt gct aag cca cct ctt gga tct ttg        144
Ala Val Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu           
                35                  40                  45                

agg ttt gcc ccg ccg caa cca gca gag cca tgg tct ttc gtt aag aac        192
Arg Phe Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn           
            50                  55                  60                    

act act tcc tac cct cca atg tgt tgt caa gat cca gtc gta gaa caa        240
Thr Thr Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Val Glu Gln           
        65                  70                  75                        

atg acg tca gac cta ttc act aac gga aag gaa agg ctt acc ctg gag        288
Met Thr Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Thr Leu Glu           
    80                  85                  90                            

ttc tct gag gat tgc ctt tac cta aat att tac act cct gcc gat ttg        336
Phe Ser Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu           
95                  100                 105                 110           

aca aag agg ggt agg ttg ccg gtt atg gtt tgg att cat gga gga ggt        384
Thr Lys Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly           
                115                 120                 125               

ttg gtt ctg ggc gga gca ccg atg tat gac gga gtg gtt ctt gcc gcg        432
Leu Val Leu Gly Gly Ala Pro Met Tyr Asp Gly Val Val Leu Ala Ala           
            130                 135                 140                   

cac gag aac gtt gtt gtt gtt gct att caa tac cgt ttg ggt att tgg        480
His Glu Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp           
        145                 150                 155                       

gga ttt ttc tcc aca gga gat gag cat tcc cgt gga aac tgg ggc cat        528
Gly Phe Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His           
    160                 165                 170                           

tta gat caa gtt gct gca ttg cat tgg gtc caa gaa aac att gct aac        576
Leu Asp Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn           
175                 180                 185                 190           

ttc gga ggt gat cca ggt tct gtt act att ttc gga gaa tca gca ggc        624
Phe Gly Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly           
                195                 200                 205               

gga gag agt gtc tct gta ttg gtt tta tca cca tta gct aag aac ctt        672
Gly Glu Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu           
            210                 215                 220                   

ttt cat cgt gct att tcc gaa agt ggt gtt gct tta acc gtc gct ttg        720
Phe His Arg Ala Ile Ser Glu Ser Gly Val Ala Leu Thr Val Ala Leu           
        225                 230                 235                       

gtc agg aag gat atg aag gcc gca gcc aag cag atc gct gtc ctt gca        768
Val Arg Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala           
    240                 245                 250                           

gga tgc aaa act act act tcg gca gtc ttc gtg cat tgt ttg cgt caa        816
Gly Cys Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln           
255                 260                 265                 270           

aag tcg gaa gat gaa ctt tta gac ctc acg ttg aag atg aaa ttc ctg        864
Lys Ser Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Leu           
                275                 280                 285               

acc ctt gac ttt cac gga gat caa agg gaa tct cac cct ttt ttg ccg        912
Thr Leu Asp Phe His Gly Asp Gln Arg Glu Ser His Pro Phe Leu Pro           
            290                 295                 300                   

act gtt gtt gac gga gtt ttg ttg cct aag atg cct gag gaa atc ttg        960
Thr Val Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu           
        305                 310                 315                       

gcc gag aag gac ttt aac acc gtc cca tac att gtt gga att aac aag       1008
Ala Glu Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys           
    320                 325                 330                           

cag gag ttc gga tgg ctt ttg cca acg atg atg gga ttt cct ctt tcc       1056
Gln Glu Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser           
335                 340                 345                 350           

gag gga aag ttg gat caa aag acg gct acg tca ctt ttg tgg aag tcc       1104
Glu Gly Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser           
                355                 360                 365               

tac cca att gcc aac att cct gaa gag ttg acc cca gtt gct acc gat       1152
Tyr Pro Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp           
            370                 375                 380                   

aag tat tta gga gga aca gat gat cct gtc aaa aag aaa gat ttg ttt       1200
Lys Tyr Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe           
        385                 390                 395                       

ttg gat ctg atg gga gac gtt gtt ttc ggc gtc cca tca gtt acg gtt       1248
Leu Asp Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val           
    400                 405                 410                           

gct cgt cag cat agg gac gca gga gct cca act tac atg tat gag ttc       1296
Ala Arg Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe           
415                 420                 425                 430           

caa tat cgt cca tct ttt tca tcg gat aag aaa cct aag acg gtt att       1344
Gln Tyr Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile           
                435                 440                 445               

gga gat cat gga gac gaa att ttt tcc gtc ttc ggc ttc cca ttg ctc       1392
Gly Asp His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu           
            450                 455                 460                   

aaa ggt gac gct cca gag gaa gaa gtc agt ctt tct aag acg gtt atg       1440
Lys Gly Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met           
        465                 470                 475                       

aaa ttt tgg gct aac ttc gcc cgt agt gga aac cct aat gga gaa gga       1488
Lys Phe Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly           
    480                 485                 490                           

ttg cct cac tgg ccg atg tac gat caa gag gag gga tac ctt caa att       1536
Leu Pro His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile           
495                 500                 505                 510           

ggt gtc aac act caa gca gct aag agg ttg aaa ggc gag gag gtt gct       1584
Gly Val Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala           
                515                 520                 525               

ttt tgg aac gac ctg ttg tcc aag gaa gca gca aag aag cca cct aag       1632
Phe Trp Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys           
            530                 535                 540                   

ata aag cac gcc gaa ttg taataagctt                                    1660
Ile Lys His Ala Glu Leu                                                   
        545                                                               


<210>  4
<211>  548
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  4

Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val 
1               5                   10                  15      


Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val 
            20                  25                  30          


Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe 
        35                  40                  45              


Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr 
    50                  55                  60                  


Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Val Glu Gln Met Thr 
65                  70                  75                  80  


Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Thr Leu Glu Phe Ser 
                85                  90                  95      


Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys 
            100                 105                 110         


Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val 
        115                 120                 125             


Leu Gly Gly Ala Pro Met Tyr Asp Gly Val Val Leu Ala Ala His Glu 
    130                 135                 140                 


Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe 
145                 150                 155                 160 


Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp 
                165                 170                 175     


Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly 
            180                 185                 190         


Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu 
        195                 200                 205             


Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His 
    210                 215                 220                 


Arg Ala Ile Ser Glu Ser Gly Val Ala Leu Thr Val Ala Leu Val Arg 
225                 230                 235                 240 


Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys 
                245                 250                 255     


Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser 
            260                 265                 270         


Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Leu Thr Leu 
        275                 280                 285             


Asp Phe His Gly Asp Gln Arg Glu Ser His Pro Phe Leu Pro Thr Val 
    290                 295                 300                 


Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu 
305                 310                 315                 320 


Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu 
                325                 330                 335     


Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly 
            340                 345                 350         


Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Tyr Pro 
        355                 360                 365             


Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr 
    370                 375                 380                 


Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp 
385                 390                 395                 400 


Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg 
                405                 410                 415     


Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr 
            420                 425                 430         


Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp 
        435                 440                 445             


His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu Lys Gly 
    450                 455                 460                 


Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe 
465                 470                 475                 480 


Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro 
                485                 490                 495     


His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val 
            500                 505                 510         


Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp 
        515                 520                 525             


Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys 
    530                 535                 540                 


His Ala Glu Leu 
545             


<210>  5
<211>  1660
<212>  DNA
<213>  Unknown

<220>
<223>  Synthetic gene


<220>
<221>  exon
<222>  (7)..(1650)
<223>  Porcine Intestinal Colon Esterase

<220>
<221>  CDS
<222>  (7)..(1650)
<223>  Porcine Intestinal Colon Esterase

<400>  5
catatg gga caa cca gct tcg ccg cct gtc gtt gat acc gct caa gga          48
       Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly            
       1               5                   10                             

cga gtc ttg ggt aag tac gtc tct tta gag gga ttg gca caa ccg gtt         96
Arg Val Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val           
15                  20                  25                  30            

gct gtc ttc ttg gga gtc cct ttt gct aag cca cct ctt gga tct ttg        144
Ala Val Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu           
                35                  40                  45                

agg ttt gcc ccg ccg caa cca gca gag cca tgg tct ttc gtt aag aac        192
Arg Phe Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn           
            50                  55                  60                    

act act tcc tac cct cca atg tgt tgt caa gat cca gtc gca gga caa        240
Thr Thr Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Ala Gly Gln           
        65                  70                  75                        

atg acg tca gac cta ttc act aac gga aag gaa agg ctt atc ccg gag        288
Met Thr Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Ile Pro Glu           
    80                  85                  90                            

ttc tct gag gat tgc ctt tac cta aat att tac act cct gcc gat ttg        336
Phe Ser Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu           
95                  100                 105                 110           

aca aag agg ggt agg ttg ccg gtt atg gtt tgg att cat gga gga ggt        384
Thr Lys Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly           
                115                 120                 125               

ttg gtt gtt ggc gga gca tcc act tat gac gga ttg gct ctt gcc gcg        432
Leu Val Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala           
            130                 135                 140                   

cac gag aac gtt gtt gtt gtt gct att caa tac cgt ttg ggt att tgg        480
His Glu Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp           
        145                 150                 155                       

gga ttt ttc tcc aca gga gat gag cat tcc cgt gga aac tgg ggc cat        528
Gly Phe Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His           
    160                 165                 170                           

tta gat caa gtt gct gca ttg cat tgg gtc caa gaa aac att gct aac        576
Leu Asp Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn           
175                 180                 185                 190           

ttc gga ggt gat aca ggt tct gtt act att ttc gga gaa tca gca ggc        624
Phe Gly Gly Asp Thr Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly           
                195                 200                 205               

gga gag agt gtc tct gta ttg gtt tta tca cca tta gct aag aac ctt        672
Gly Glu Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu           
            210                 215                 220                   

ttt cat cgt gct att tcc gaa agt ggt gtt gct tta acc gcc ggt ttg        720
Phe His Arg Ala Ile Ser Glu Ser Gly Val Ala Leu Thr Ala Gly Leu           
        225                 230                 235                       

gtc agg aag gat atg aag gcc gca gcc aag cag atc gct gtc ctt gca        768
Val Arg Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala           
    240                 245                 250                           

gga tgc aaa act act act tcg gca gtc ttc gtg cat tgt ttg cgt caa        816
Gly Cys Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln           
255                 260                 265                 270           

aag tcg gaa gat gaa ctt tta gac ctc acg ttg aag atg aaa ttc ctg        864
Lys Ser Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Leu           
                275                 280                 285               

acc ctt gac tta cac gga gat cca agg gaa tct cac cct ttt ttg ccg        912
Thr Leu Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Pro           
            290                 295                 300                   

act gtt gtt gac gga gtt ttg ttg cct aag atg cct gag gaa atc ttg        960
Thr Val Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu           
        305                 310                 315                       

gcc gag aag gac ttt aac acc gtc cca tac att gtt gga att aac aag       1008
Ala Glu Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys           
    320                 325                 330                           

cag gag ttc gga tgg ctt ttg cca acg atg atg gga ttt cct ctt tcc       1056
Gln Glu Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser           
335                 340                 345                 350           

gag gga aag ttg gat caa aag acg gct acg tca ctt ttg tgg aag tcc       1104
Glu Gly Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser           
                355                 360                 365               

ttc cca att acc aac att cct gaa gag ttg acc cca gtt gct acc gat       1152
Phe Pro Ile Thr Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp           
            370                 375                 380                   

aag tat tta gga gga aca gat gat cct gtc aaa aag aaa gat ttg ttt       1200
Lys Tyr Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe           
        385                 390                 395                       

ttg gat ctg atg gga gac gtt gtt ttc ggc gtc cca tca gtt acg gtt       1248
Leu Asp Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val           
    400                 405                 410                           

gct cgt cag cat agg gac gca gga gct cca act tac atg tat gag ttc       1296
Ala Arg Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe           
415                 420                 425                 430           

caa tat cgt cca tct ttt tca tcg gat aag aaa cct aag acg gtt att       1344
Gln Tyr Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile           
                435                 440                 445               

gga gat cat gga gac gaa att ttt tcc gtc ttc ggc ttc cca ttg ctc       1392
Gly Asp His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu           
            450                 455                 460                   

aaa ggt gac gct cca gag gaa gaa gtc agt ctt tct aag acg gtt atg       1440
Lys Gly Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met           
        465                 470                 475                       

aaa ttt tgg gct aac ttc gcc cgt agt gga aac cct aat gga gaa gga       1488
Lys Phe Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly           
    480                 485                 490                           

ttg cct cac tgg ccg atg tac gat caa gag gag gga tac ctt caa att       1536
Leu Pro His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile           
495                 500                 505                 510           

ggt gtc aac act caa gca gct aag agg ttg aaa ggc gag gag gtt gct       1584
Gly Val Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala           
                515                 520                 525               

ttt tgg aac gac ctg ttg tcc aag gaa gca gca aag aag cca cct aag       1632
Phe Trp Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys           
            530                 535                 540                   

ata aag cac gcc gaa ttg taataagctt                                    1660
Ile Lys His Ala Glu Leu                                                   
        545                                                               


<210>  6
<211>  548
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  6

Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val 
1               5                   10                  15      


Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val 
            20                  25                  30          


Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe 
        35                  40                  45              


Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr 
    50                  55                  60                  


Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Ala Gly Gln Met Thr 
65                  70                  75                  80  


Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Ile Pro Glu Phe Ser 
                85                  90                  95      


Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys 
            100                 105                 110         


Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val 
        115                 120                 125             


Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala His Glu 
    130                 135                 140                 


Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe 
145                 150                 155                 160 


Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp 
                165                 170                 175     


Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly 
            180                 185                 190         


Gly Asp Thr Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu 
        195                 200                 205             


Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His 
    210                 215                 220                 


Arg Ala Ile Ser Glu Ser Gly Val Ala Leu Thr Ala Gly Leu Val Arg 
225                 230                 235                 240 


Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys 
                245                 250                 255     


Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser 
            260                 265                 270         


Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Leu Thr Leu 
        275                 280                 285             


Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Pro Thr Val 
    290                 295                 300                 


Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu 
305                 310                 315                 320 


Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu 
                325                 330                 335     


Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly 
            340                 345                 350         


Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Phe Pro 
        355                 360                 365             


Ile Thr Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr 
    370                 375                 380                 


Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp 
385                 390                 395                 400 


Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg 
                405                 410                 415     


Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr 
            420                 425                 430         


Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp 
        435                 440                 445             


His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu Lys Gly 
    450                 455                 460                 


Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe 
465                 470                 475                 480 


Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro 
                485                 490                 495     


His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val 
            500                 505                 510         


Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp 
        515                 520                 525             


Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys 
    530                 535                 540                 


His Ala Glu Leu 
545             


<210>  7
<211>  1660
<212>  DNA
<213>  Unknown

<220>
<223>  Synthetic gene


<220>
<221>  exon
<222>  (7)..(1650)
<223>  Pig Liver Esrterase 2

<220>
<221>  CDS
<222>  (7)..(1650)
<223>  Pig Liver Esrterase 2

<400>  7
catatg gga caa cca gct tcg ccg cct gtc gtt gat acc gct caa gga          48
       Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly            
       1               5                   10                             

cga gtc ttg ggt aag tac gtc tct tta gag gga ttg gca caa ccg gtt         96
Arg Val Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val           
15                  20                  25                  30            

gct gtc ttc ttg gga gtc cct ttt gct aag cca cct ctt gga tct ttg        144
Ala Val Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu           
                35                  40                  45                

agg ttt gcc ccg ccg caa cca gca gag cca tgg tct ttc gtt aag aac        192
Arg Phe Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn           
            50                  55                  60                    

act act tcc tac cct cca atg tgt tgt caa gat cca gtc gta gaa caa        240
Thr Thr Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Val Glu Gln           
        65                  70                  75                        

atg acg tca gac cta ttc act aac gga aag gaa agg ctt acc ctg gag        288
Met Thr Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Thr Leu Glu           
    80                  85                  90                            

ttc tct gag gat tgc ctt tac cta aat att tac act cct gcc gat ttg        336
Phe Ser Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu           
95                  100                 105                 110           

aca aag agg ggt agg ttg ccg gtt atg gtt tgg att cat gga gga ggt        384
Thr Lys Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly           
                115                 120                 125               

ttg gtt ctg ggc gga gca ccg atg tat gac gga gtg gtt ctt gcc gcg        432
Leu Val Leu Gly Gly Ala Pro Met Tyr Asp Gly Val Val Leu Ala Ala           
            130                 135                 140                   

cac gag aac gtt gtt gtt gtt gct att caa tac cgt ttg ggt att tgg        480
His Glu Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp           
        145                 150                 155                       

gga ttt ttc tcc aca gga gat gag cat tcc cgt gga aac tgg ggc cat        528
Gly Phe Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His           
    160                 165                 170                           

tta gat caa gtt gct gca ttg cat tgg gtc caa gaa aac att gct aac        576
Leu Asp Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn           
175                 180                 185                 190           

ttc gga ggt gat cca ggt tct gtt act att ttc gga gaa tca gca ggc        624
Phe Gly Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly           
                195                 200                 205               

gga gag agt gtc tct gta ttg gtt tta tca cca tta gct aag aac ctt        672
Gly Glu Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu           
            210                 215                 220                   

ttt cat cgt gct att tcc gaa agt ggt gtt gct tta acc gtc gct ttg        720
Phe His Arg Ala Ile Ser Glu Ser Gly Val Ala Leu Thr Val Ala Leu           
        225                 230                 235                       

gtc agg aag gat atg aag gcc gca gcc aag cag atc gct gtc ctt gca        768
Val Arg Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala           
    240                 245                 250                           

gga tgc aaa act act act tcg gca gtc ttc gtg cat tgt ttg cgt caa        816
Gly Cys Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln           
255                 260                 265                 270           

aag tcg gaa gat gaa ctt tta gac ctc acg ttg aag atg aaa ttc ctg        864
Lys Ser Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Leu           
                275                 280                 285               

acc ctt gac ttt cac gga gat caa agg gaa tct cac cct ttt ttg ccg        912
Thr Leu Asp Phe His Gly Asp Gln Arg Glu Ser His Pro Phe Leu Pro           
            290                 295                 300                   

act gtt gtt gac gga gtt ttg ttg cct aag atg cct gag gaa atc ttg        960
Thr Val Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu           
        305                 310                 315                       

gcc gag aag gac ttt aac acc gtc cca tac att gtt gga att aac aag       1008
Ala Glu Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys           
    320                 325                 330                           

cag gag ttc gga tgg ctt ttg cca acg atg atg gga ttt cct ctt tcc       1056
Gln Glu Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser           
335                 340                 345                 350           

gag gga aag ttg gat caa aag acg gct acg tca ctt ttg tgg aag tcc       1104
Glu Gly Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser           
                355                 360                 365               

tac cca att gcc aac att cct gaa gag ttg acc cca gtt gct acc gat       1152
Tyr Pro Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp           
            370                 375                 380                   

aag tat tta gga gga aca gat gat cct gtc aaa aag aaa gat ttg ttt       1200
Lys Tyr Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe           
        385                 390                 395                       

ttg gat ctg atg gga gac gtt gtt ttc ggc gtc cca tca gtt acg gtt       1248
Leu Asp Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val           
    400                 405                 410                           

gct cgt cag cat agg gac gca gga gct cca act tac atg tat gag ttc       1296
Ala Arg Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe           
415                 420                 425                 430           

caa tat cgt cca tct ttt tca tcg gat aag aaa cct aag acg gtt att       1344
Gln Tyr Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile           
                435                 440                 445               

gga gat cat gga gac gaa att ttt tcc gtc ttc ggc gcc cca ttc ctc       1392
Gly Asp His Gly Asp Glu Ile Phe Ser Val Phe Gly Ala Pro Phe Leu           
            450                 455                 460                   

aga ggt gac gct cca gag gaa gaa gtc agt ctt tct aag acg gtt atg       1440
Arg Gly Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met           
        465                 470                 475                       

aaa ttt tgg gct aac ttc gcc cgt agt gga aac cct aat gga gaa gga       1488
Lys Phe Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly           
    480                 485                 490                           

ttg cct cac tgg ccg atg tac gat caa gag gag gga tac ctt caa att       1536
Leu Pro His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile           
495                 500                 505                 510           

ggt gtc aac act caa gca gct aag agg ttg aaa ggc gag gag gtt gct       1584
Gly Val Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala           
                515                 520                 525               

ttt tgg aac gac ctg ttg tcc aag gaa gca gca aag aag cca cct aag       1632
Phe Trp Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys           
            530                 535                 540                   

ata aag cac gcc gaa ttg taataagctt                                    1660
Ile Lys His Ala Glu Leu                                                   
        545                                                               


<210>  8
<211>  548
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  8

Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val 
1               5                   10                  15      


Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val 
            20                  25                  30          


Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe 
        35                  40                  45              


Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr 
    50                  55                  60                  


Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Val Glu Gln Met Thr 
65                  70                  75                  80  


Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Thr Leu Glu Phe Ser 
                85                  90                  95      


Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys 
            100                 105                 110         


Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val 
        115                 120                 125             


Leu Gly Gly Ala Pro Met Tyr Asp Gly Val Val Leu Ala Ala His Glu 
    130                 135                 140                 


Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe 
145                 150                 155                 160 


Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp 
                165                 170                 175     


Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly 
            180                 185                 190         


Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu 
        195                 200                 205             


Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His 
    210                 215                 220                 


Arg Ala Ile Ser Glu Ser Gly Val Ala Leu Thr Val Ala Leu Val Arg 
225                 230                 235                 240 


Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys 
                245                 250                 255     


Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser 
            260                 265                 270         


Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Leu Thr Leu 
        275                 280                 285             


Asp Phe His Gly Asp Gln Arg Glu Ser His Pro Phe Leu Pro Thr Val 
    290                 295                 300                 


Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu 
305                 310                 315                 320 


Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu 
                325                 330                 335     


Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly 
            340                 345                 350         


Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Tyr Pro 
        355                 360                 365             


Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr 
    370                 375                 380                 


Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp 
385                 390                 395                 400 


Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg 
                405                 410                 415     


Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr 
            420                 425                 430         


Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp 
        435                 440                 445             


His Gly Asp Glu Ile Phe Ser Val Phe Gly Ala Pro Phe Leu Arg Gly 
    450                 455                 460                 


Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe 
465                 470                 475                 480 


Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro 
                485                 490                 495     


His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val 
            500                 505                 510         


Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp 
        515                 520                 525             


Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys 
    530                 535                 540                 


His Ala Glu Leu 
545             


<210>  9
<211>  1660
<212>  DNA
<213>  Unknown

<220>
<223>  Synthetic gene


<220>
<221>  exon
<222>  (7)..(1650)
<223>  Pig Liver Esterase 3

<220>
<221>  CDS
<222>  (7)..(1650)
<223>  Pig Liver Esterase 3

<400>  9
catatg gga caa cca gct tcg ccg cct gtc gtt gat acc gct caa gga          48
       Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly            
       1               5                   10                             

cga gtc ttg ggt aag tac gtc tct tta gag gga ttg gca caa ccg gtt         96
Arg Val Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val           
15                  20                  25                  30            

gct gtc ttc ttg gga gtc cct ttt gct aag cca cct ctt gga tct ttg        144
Ala Val Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu           
                35                  40                  45                

agg ttt gcc ccg ccg caa cca gca gag cca tgg tct ttc gtt aag aac        192
Arg Phe Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn           
            50                  55                  60                    

act act tcc tac cct cca atg tgt tgt caa gaa cca atc gga gga caa        240
Thr Thr Ser Tyr Pro Pro Met Cys Cys Gln Glu Pro Ile Gly Gly Gln           
        65                  70                  75                        

atg ctt tca gac cta ttc act aac aga aag gaa agg ctt atc ccg gag        288
Met Leu Ser Asp Leu Phe Thr Asn Arg Lys Glu Arg Leu Ile Pro Glu           
    80                  85                  90                            

ttc tct gag gat tgc ctt tac cta aat att tac act cct gcc gat ttg        336
Phe Ser Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu           
95                  100                 105                 110           

aca aag agg ggt agg ttg ccg gtt atg gtt tgg att cat gga gga ggt        384
Thr Lys Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly           
                115                 120                 125               

ttg gtt gtt ggc gga gca tcc act tat gac gga ttg gct ctt gcc gcg        432
Leu Val Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala           
            130                 135                 140                   

cac gag aac gtt gtt gtt gtt gct att caa tac cgt ttg ggt att tgg        480
His Glu Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp           
        145                 150                 155                       

gga ttt ttc tcc aca gga gat gag cat tcc cgt gga aac tgg ggc cat        528
Gly Phe Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His           
    160                 165                 170                           

tta gat caa gtt gct gca ttg cat tgg gtc caa gaa aac att gct aac        576
Leu Asp Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn           
175                 180                 185                 190           

ttc gga ggt gat cca ggt tct gtt act att ttc gga gaa tca gca ggc        624
Phe Gly Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly           
                195                 200                 205               

gga gag agt gtc tct gta ttg gtt tta tca cca tta gct aag aac ctt        672
Gly Glu Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu           
            210                 215                 220                   

ttt cat cgt gct att tcc gaa agt ggt gtt gct ttt acc gcc ggt ttg        720
Phe His Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu           
        225                 230                 235                       

gtc agg aag gat atg aag gcc gca gcc aag cag atc gct gtc ctt gca        768
Val Arg Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala           
    240                 245                 250                           

gga tgc aaa act act act tcg gca gtc ttc gtg cat tgt ttg cgt caa        816
Gly Cys Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln           
255                 260                 265                 270           

aag tcg gaa gat gaa ctt tta gac ctc acg ttg aag atg aaa ccc tta        864
Lys Ser Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Pro Leu           
                275                 280                 285               

acc ctt gac tta cac gga gat cca agg gaa tct cac cct ttt ttg acc        912
Thr Leu Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr           
            290                 295                 300                   

act gtt gtt gac gga gtt ttg ttg cct aag atg cct gag gaa atc ttg        960
Thr Val Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu           
        305                 310                 315                       

gcc gag aag gac ttt aac acc gtc cca tac att gtt gga att aac aag       1008
Ala Glu Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys           
    320                 325                 330                           

cag gag ttc gga tgg ctt ttg cca acg atg atg gga ttt cct ctt tcc       1056
Gln Glu Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser           
335                 340                 345                 350           

gag gga aag ttg gat caa aag acg gct acg tca ctt ttg tgg aag tcc       1104
Glu Gly Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser           
                355                 360                 365               

tac cca att gcc aac att cct gaa gag ttg acc cca gtt gct acc gat       1152
Tyr Pro Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp           
            370                 375                 380                   

aag tat tta gga gga aca gat gat cct gtc aaa aag aaa gat ttg ttt       1200
Lys Tyr Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe           
        385                 390                 395                       

ttg gat ctg atg gga gac gtt gtt ttc ggc gtc cca tca gtt acg gtt       1248
Leu Asp Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val           
    400                 405                 410                           

gct cgt cag cat agg gac gca gga gct cca act tac atg tat gag ttc       1296
Ala Arg Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe           
415                 420                 425                 430           

caa tat cgt cca tct ttt tca tcg gat aag aaa cct aag acg gtt att       1344
Gln Tyr Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile           
                435                 440                 445               

gga gat cat gga gac gaa att ttt tcc gtc ttc ggc ttc cca ttg ctc       1392
Gly Asp His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu           
            450                 455                 460                   

aaa ggt gac gct cca gag gaa gaa gtc agt ctt tct aag acg gtt atg       1440
Lys Gly Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met           
        465                 470                 475                       

aaa ttt tgg gct aac ttc gcc cgt agt gga aac cct aat gga gaa gga       1488
Lys Phe Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly           
    480                 485                 490                           

ttg cct cac tgg ccg atg tac gat caa gag gag gga tac ctt caa att       1536
Leu Pro His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile           
495                 500                 505                 510           

ggt gtc aac act caa gca gct aag agg ttg aaa ggc gag gag gtt gct       1584
Gly Val Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala           
                515                 520                 525               

ttt tgg aac gac ctg ttg tcc aag gaa gca gca aag aag cca cct aag       1632
Phe Trp Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys           
            530                 535                 540                   

ata aag cac gcc gaa ttg taataagctt                                    1660
Ile Lys His Ala Glu Leu                                                   
        545                                                               


<210>  10
<211>  548
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  10

Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val 
1               5                   10                  15      


Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val 
            20                  25                  30          


Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe 
        35                  40                  45              


Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr 
    50                  55                  60                  


Ser Tyr Pro Pro Met Cys Cys Gln Glu Pro Ile Gly Gly Gln Met Leu 
65                  70                  75                  80  


Ser Asp Leu Phe Thr Asn Arg Lys Glu Arg Leu Ile Pro Glu Phe Ser 
                85                  90                  95      


Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys 
            100                 105                 110         


Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val 
        115                 120                 125             


Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala His Glu 
    130                 135                 140                 


Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe 
145                 150                 155                 160 


Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp 
                165                 170                 175     


Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly 
            180                 185                 190         


Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu 
        195                 200                 205             


Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His 
    210                 215                 220                 


Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu Val Arg 
225                 230                 235                 240 


Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys 
                245                 250                 255     


Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser 
            260                 265                 270         


Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Pro Leu Thr Leu 
        275                 280                 285             


Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr Thr Val 
    290                 295                 300                 


Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu 
305                 310                 315                 320 


Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu 
                325                 330                 335     


Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly 
            340                 345                 350         


Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Tyr Pro 
        355                 360                 365             


Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr 
    370                 375                 380                 


Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp 
385                 390                 395                 400 


Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg 
                405                 410                 415     


Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr 
            420                 425                 430         


Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp 
        435                 440                 445             


His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu Lys Gly 
    450                 455                 460                 


Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe 
465                 470                 475                 480 


Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro 
                485                 490                 495     


His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val 
            500                 505                 510         


Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp 
        515                 520                 525             


Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys 
    530                 535                 540                 


His Ala Glu Leu 
545             


<210>  11
<211>  1660
<212>  DNA
<213>  Unknown

<220>
<223>  Synthetic gene


<220>
<221>  exon
<222>  (7)..(1650)
<223>  Pig Liver Esterase 4

<220>
<221>  CDS
<222>  (7)..(1650)
<223>  Pig Liver Esterase 4

<400>  11
catatg gga caa cca gct tcg ccg cct gtc gtt gat acc gct caa gga          48
       Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly            
       1               5                   10                             

cga gtc ttg ggt aag tac gtc tct tta gag gga ttg gca caa ccg gtt         96
Arg Val Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val           
15                  20                  25                  30            

gct gtc ttc ttg gga gtc cct ttt gct aag cca cct ctt gga tct ttg        144
Ala Val Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu           
                35                  40                  45                

agg ttt gcc ccg ccg caa cca gca gag cca tgg tct ttc gtt aag aac        192
Arg Phe Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn           
            50                  55                  60                    

act act tcc tac cct cca atg tgt tgt caa gat cca gtc gca gga caa        240
Thr Thr Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Ala Gly Gln           
        65                  70                  75                        

atg acg tca gac cta ttc act aac gga aag gaa agg ctt atc ccg gag        288
Met Thr Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Ile Pro Glu           
    80                  85                  90                            

ttc tct gag gat tgc ctt tac cta aat att tac act cct gcc gat ttg        336
Phe Ser Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu           
95                  100                 105                 110           

aca aag agg ggt agg ttg ccg gtt atg gtt tgg att cat gga gga ggt        384
Thr Lys Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly           
                115                 120                 125               

ttg gtt gtt ggc gga gca tcc act tat gac gga ttg gct ctt gcc gcg        432
Leu Val Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala           
            130                 135                 140                   

cac gag aac gtt gtt gtt gtt gct att caa tac cgt ttg ggt att tgg        480
His Glu Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp           
        145                 150                 155                       

gga ttt ttc tcc aca gga gat gag cat tcc cgt gga aac tgg ggc cat        528
Gly Phe Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His           
    160                 165                 170                           

tta gat caa gtt gct gca ttg cat tgg gtc caa gaa aac att gct aac        576
Leu Asp Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn           
175                 180                 185                 190           

ttc gga ggt gat cca ggt tct gtt act att ttc gga gaa tca gca ggc        624
Phe Gly Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly           
                195                 200                 205               

gga gag agt gtc tct gta ttg gtt tta tca cca tta gct aag aac ctt        672
Gly Glu Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu           
            210                 215                 220                   

ttt cat cgt gct att tcc gaa agt ggt gtt gct ttt acc gcc ggt ttg        720
Phe His Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu           
        225                 230                 235                       

gtc agg aag gat atg aag gcc gca gcc aag cag atc gct gtc ctt gca        768
Val Arg Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala           
    240                 245                 250                           

gga tgc aaa act act act tcg gca gtc ttc gtg cat tgt ttg cgt caa        816
Gly Cys Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln           
255                 260                 265                 270           

aag tcg gaa gat gaa ctt tta gac ctc acg ttg aag atg aaa ccc tta        864
Lys Ser Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Pro Leu           
                275                 280                 285               

acc ctt gac tta cac gga gat cca agg gaa tct cac cct ttt ttg acc        912
Thr Leu Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr           
            290                 295                 300                   

act gtt gtt gac gga gtt ttg ttg cct aag atg cct gag gaa atc ttg        960
Thr Val Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu           
        305                 310                 315                       

gcc gag aag gac ttt aac acc gtc cca tac att gtt gga att aac aag       1008
Ala Glu Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys           
    320                 325                 330                           

cag gag ttc gga tgg ctt ttg cca acg atg atg gga ttt cct ctt tcc       1056
Gln Glu Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser           
335                 340                 345                 350           

gag gga aag ttg gat caa aag acg gct acg tca ctt ttg tgg aag tcc       1104
Glu Gly Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser           
                355                 360                 365               

tac cca att gcc aac att cct gaa gag ttg acc cca gtt gct acc gat       1152
Tyr Pro Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp           
            370                 375                 380                   

aag tat tta gga gga aca gat gat cct gtc aaa aag aaa gat ttg ttt       1200
Lys Tyr Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe           
        385                 390                 395                       

ttg gat ctg atg gga gac gtt gtt ttc ggc gtc cca tca gtt acg gtt       1248
Leu Asp Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val           
    400                 405                 410                           

gct cgt cag cat agg gac gca gga gct cca act tac atg tat gag ttc       1296
Ala Arg Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe           
415                 420                 425                 430           

caa tat cgt cca tct ttt tca tcg gat aag aaa cct aag acg gtt att       1344
Gln Tyr Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile           
                435                 440                 445               

gga gat cat gga gac gaa att ttt tcc gtc ttc ggc gcc cca ttc ctc       1392
Gly Asp His Gly Asp Glu Ile Phe Ser Val Phe Gly Ala Pro Phe Leu           
            450                 455                 460                   

aga ggt gac gct cca gag gaa gaa gtc agt ctt tct aag acg gtt atg       1440
Arg Gly Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met           
        465                 470                 475                       

aaa ttt tgg gct aac ttc gcc cgt agt gga aac cct aat gga gaa gga       1488
Lys Phe Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly           
    480                 485                 490                           

ttg cct cac tgg ccg atg tac gat caa gag gag gga tac ctt caa att       1536
Leu Pro His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile           
495                 500                 505                 510           

ggt gtc aac act caa gca gct aag agg ttg aaa ggc gag gag gtt gct       1584
Gly Val Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala           
                515                 520                 525               

ttt tgg aac gac ctg ttg tcc aag gaa gca gca aag aag cca cct aag       1632
Phe Trp Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys           
            530                 535                 540                   

ata aag cac gcc gaa ttg taataagctt                                    1660
Ile Lys His Ala Glu Leu                                                   
        545                                                               


<210>  12
<211>  548
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  12

Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val 
1               5                   10                  15      


Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val 
            20                  25                  30          


Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe 
        35                  40                  45              


Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr 
    50                  55                  60                  


Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Ala Gly Gln Met Thr 
65                  70                  75                  80  


Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Ile Pro Glu Phe Ser 
                85                  90                  95      


Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys 
            100                 105                 110         


Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val 
        115                 120                 125             


Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala His Glu 
    130                 135                 140                 


Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe 
145                 150                 155                 160 


Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp 
                165                 170                 175     


Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly 
            180                 185                 190         


Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu 
        195                 200                 205             


Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His 
    210                 215                 220                 


Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu Val Arg 
225                 230                 235                 240 


Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys 
                245                 250                 255     


Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser 
            260                 265                 270         


Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Pro Leu Thr Leu 
        275                 280                 285             


Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr Thr Val 
    290                 295                 300                 


Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu 
305                 310                 315                 320 


Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu 
                325                 330                 335     


Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly 
            340                 345                 350         


Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Tyr Pro 
        355                 360                 365             


Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr 
    370                 375                 380                 


Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp 
385                 390                 395                 400 


Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg 
                405                 410                 415     


Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr 
            420                 425                 430         


Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp 
        435                 440                 445             


His Gly Asp Glu Ile Phe Ser Val Phe Gly Ala Pro Phe Leu Arg Gly 
    450                 455                 460                 


Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe 
465                 470                 475                 480 


Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro 
                485                 490                 495     


His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val 
            500                 505                 510         


Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp 
        515                 520                 525             


Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys 
    530                 535                 540                 


His Ala Glu Leu 
545             


<210>  13
<211>  1660
<212>  DNA
<213>  Unknown

<220>
<223>  Synthic gene


<220>
<221>  exon
<222>  (7)..(1650)
<223>  Pig Liver esterase 5

<220>
<221>  CDS
<222>  (7)..(1650)
<223>  Pig Liver esterase 5

<400>  13
catatg gga caa cca gct tcg ccg cct gtc gtt gat acc gct caa gga          48
       Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly            
       1               5                   10                             

cga gtc ttg ggt aag tac gtc tct tta gag gga ttg gca caa ccg gtt         96
Arg Val Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val           
15                  20                  25                  30            

gct gtc ttc ttg gga gtc cct ttt gct aag cca cct ctt gga tct ttg        144
Ala Val Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu           
                35                  40                  45                

agg ttt gcc ccg ccg caa cca gca gag cca tgg tct ttc gtt aag aac        192
Arg Phe Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn           
            50                  55                  60                    

act act tcc tac cct cca atg tgt tgt caa gat cca gtc gca gga caa        240
Thr Thr Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Ala Gly Gln           
        65                  70                  75                        

atg acg tca gac cta ttc act aac gga aag gaa agg ctt atc ccg gag        288
Met Thr Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Ile Pro Glu           
    80                  85                  90                            

ttc tct gag gat tgc ctt tac cta aat att tac act cct gcc gat ttg        336
Phe Ser Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu           
95                  100                 105                 110           

aca aag agg ggt agg ttg ccg gtt atg gtt tgg att cat gga gga ggt        384
Thr Lys Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly           
                115                 120                 125               

ttg gtt gtt ggc gga gca tcc act tat gac gga ttg gct ctt gcc gcg        432
Leu Val Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala           
            130                 135                 140                   

cac gag aac gtt gtt gtt gtt gct att caa tac cgt ttg ggt att tgg        480
His Glu Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp           
        145                 150                 155                       

gga ttt ttc tcc aca gga gat gag cat tcc cgt gga aac tgg ggc cat        528
Gly Phe Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His           
    160                 165                 170                           

tta gat caa gtt gct gca ttg cat tgg gtc caa gaa aac att gct aac        576
Leu Asp Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn           
175                 180                 185                 190           

ttc gga ggt gat cca ggt tct gtt act att ttc gga gaa tca gca ggc        624
Phe Gly Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly           
                195                 200                 205               

gga gag agt gtc tct gta ttg gtt tta tca cca tta gct aag aac ctt        672
Gly Glu Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu           
            210                 215                 220                   

ttt cat cgt gct att tcc gaa agt ggt gtt gct ttt acc gcc ggt ttg        720
Phe His Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu           
        225                 230                 235                       

gtc agg aag gat atg aag gcc gca gcc aag cag atc gct gtc ctt gca        768
Val Arg Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala           
    240                 245                 250                           

gga tgc aaa act act act tcg gca gtc ttc gtg cat tgt ttg cgt caa        816
Gly Cys Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln           
255                 260                 265                 270           

aag tcg gaa gat gaa ctt tta gac ctc acg ttg aag atg aaa ttc ttt        864
Lys Ser Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Phe           
                275                 280                 285               

gcc ctt gac tta cac gga gat cca agg gaa tct cac cct ttt ttg acc        912
Ala Leu Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr           
            290                 295                 300                   

act gtt gtt gac gga gtt ttg ttg cct aag atg cct gag gaa atc ttg        960
Thr Val Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu           
        305                 310                 315                       

gcc gag aag gac ttt aac acc gtc cca tac att gtt gga att aac aag       1008
Ala Glu Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys           
    320                 325                 330                           

cag gag ttc gga tgg ctt ttg cca acg atg atg gga ttt cct ctt tcc       1056
Gln Glu Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser           
335                 340                 345                 350           

gag gga aag ttg gat caa aag acg gct acg tca ctt ttg tgg aag tcc       1104
Glu Gly Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser           
                355                 360                 365               

tac cca att gcc aac att cct gaa gag ttg acc cca gtt gct acc gat       1152
Tyr Pro Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp           
            370                 375                 380                   

aag tat tta gga gga aca gat gat cct gtc aaa aag aaa gat ttg ttt       1200
Lys Tyr Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe           
        385                 390                 395                       

ttg gat ctg atg gga gac gtt gtt ttc ggc gtc cca tca gtt acg gtt       1248
Leu Asp Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val           
    400                 405                 410                           

gct cgt cag cat agg gac gca gga gct cca act tac atg tat gag ttc       1296
Ala Arg Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe           
415                 420                 425                 430           

caa tat cgt cca tct ttt tca tcg gat aag aaa cct aag acg gtt att       1344
Gln Tyr Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile           
                435                 440                 445               

gga gat cat gga gac gaa att ttt tcc gtc ttc ggc gcc cca ttc ctc       1392
Gly Asp His Gly Asp Glu Ile Phe Ser Val Phe Gly Ala Pro Phe Leu           
            450                 455                 460                   

aga ggt gac gct cca gag gaa gaa gtc agt ctt tct aag acg gtt atg       1440
Arg Gly Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met           
        465                 470                 475                       

aaa ttt tgg gct aac ttc gcc cgt agt gga aac cct aat gga gaa gga       1488
Lys Phe Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly           
    480                 485                 490                           

ttg cct cac tgg ccg atg tac gat caa gag gag gga tac ctt caa att       1536
Leu Pro His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile           
495                 500                 505                 510           

ggt gtc aac act caa gca gct aag agg ttg aaa ggc gag gag gtt gct       1584
Gly Val Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala           
                515                 520                 525               

ttt tgg aac gac ctg ttg tcc aag gaa gca gca aag aag cca cct aag       1632
Phe Trp Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys           
            530                 535                 540                   

ata aag cac gcc gaa ttg taataagctt                                    1660
Ile Lys His Ala Glu Leu                                                   
        545                                                               


<210>  14
<211>  548
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  14

Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val 
1               5                   10                  15      


Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val 
            20                  25                  30          


Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe 
        35                  40                  45              


Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr 
    50                  55                  60                  


Ser Tyr Pro Pro Met Cys Cys Gln Asp Pro Val Ala Gly Gln Met Thr 
65                  70                  75                  80  


Ser Asp Leu Phe Thr Asn Gly Lys Glu Arg Leu Ile Pro Glu Phe Ser 
                85                  90                  95      


Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys 
            100                 105                 110         


Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val 
        115                 120                 125             


Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala His Glu 
    130                 135                 140                 


Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe 
145                 150                 155                 160 


Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp 
                165                 170                 175     


Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly 
            180                 185                 190         


Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu 
        195                 200                 205             


Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His 
    210                 215                 220                 


Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu Val Arg 
225                 230                 235                 240 


Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys 
                245                 250                 255     


Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser 
            260                 265                 270         


Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Phe Ala Leu 
        275                 280                 285             


Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr Thr Val 
    290                 295                 300                 


Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu 
305                 310                 315                 320 


Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu 
                325                 330                 335     


Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly 
            340                 345                 350         


Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Tyr Pro 
        355                 360                 365             


Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr 
    370                 375                 380                 


Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp 
385                 390                 395                 400 


Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg 
                405                 410                 415     


Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr 
            420                 425                 430         


Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp 
        435                 440                 445             


His Gly Asp Glu Ile Phe Ser Val Phe Gly Ala Pro Phe Leu Arg Gly 
    450                 455                 460                 


Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe 
465                 470                 475                 480 


Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro 
                485                 490                 495     


His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val 
            500                 505                 510         


Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp 
        515                 520                 525             


Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys 
    530                 535                 540                 


His Ala Glu Leu 
545             


<210>  15
<211>  41
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for L43D F

<400>  15
gtcccttttg ctaagccacc tgacggatct ttgaggtttg c                           41


<210>  16
<211>  41
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for L43D R

<400>  16
gcaaacctca aagatccgtc aggtggctta gcaaaaggga c                           41


<210>  17
<211>  37
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for T260P F

<400>  17
gcaggatgca aaactactcc ttcggcagtc ttcgtgc                                37


<210>  18
<211>  37
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for T260P R

<400>  18
gcacgaagac tgccgaagga gtagttttgc atcctgc                                37


<210>  19
<211>  37
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for T260A F

<400>  19
gcaggatgca aaactactgc ttcggcagtc ttcgtgc                                37


<210>  20
<211>  37
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for T260A R

<400>  20
gcacgaagac tgccgaagca gtagttttgc atcctgc                                37


<210>  21
<211>  33
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for T263D F

<400>  21
ctactacttc ggcagacttc gtgcattgtt tgc                                    33


<210>  22
<211>  33
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for T263D R

<400>  22
gcaaacaatg cacgaagtct gccgaagtag tag                                    33


<210>  23
<211>  41
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for V263G F

<400>  23
caaaactact acttcggcag ggttcgtgca ttgtttgcgt c                           41


<210>  24
<211>  41
<212>  DNA
<213>  Unknown

<220>
<223>  Primer for V263G R

<400>  24
gacgcaaaca atgcacgaac cctgccgaag tagtagtttt g                           41


