
                                SEQUENCE LISTING

<110> Huh, Jin Hoe
      Fischer, Robert
      The Regents of the University of California

<120> ENGINEERED DEMETER 5-METHYLCYTOSINE DNA
      GLYCOSYLASE WITH IMPROVED YIELD, STABILITY AND SOLUBILITY
  

<130> 02307O-198510PC

<150> US 61/170,978       
<151> 2009-04-20  

<160> 14

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 120
<212> PRT
<213> Arabidopsis thaliana

<220> 
<223> positions 678 to 797 of DME gene

<400> 1
Tyr Lys Gly Asp Gly Ala Leu Val Pro Tyr Glu Ser Lys Lys Arg Lys
 1               5                  10                  15      
Pro Arg Pro Lys Val Asp Ile Asp Asp Glu Thr Thr Arg Ile Trp Asn
            20                  25                  30          
Leu Leu Met Gly Lys Gly Asp Glu Lys Glu Gly Asp Glu Glu Lys Asp
        35                  40                  45              
Lys Lys Lys Glu Lys Trp Trp Glu Glu Glu Arg Arg Val Phe Arg Gly
    50                  55                  60                  
Arg Ala Asp Ser Phe Ile Ala Arg Met His Leu Val Gln Gly Asp Arg
65                  70                  75                  80  
Arg Phe Ser Pro Trp Lys Gly Ser Val Val Asp Ser Val Ile Gly Val
                85                  90                  95      
Phe Leu Thr Gln Asn Val Ser Asp His Leu Ser Ser Ser Ala Phe Met
            100                 105                 110         
Ser Leu Ala Ala Arg Phe Pro Pro
        115                 120 


<210> 2
<211> 541
<212> PRT
<213> Arabidopsis thaliana

<220> 
<223> positions 1189 to 1729 of DME gene

<400> 2
Thr Ser Gln Trp Asp Ser Leu Arg Lys Asp Val Glu Gly Asn Glu Gly
 1               5                  10                  15      
Arg Gln Glu Arg Asn Lys Asn Asn Met Asp Ser Ile Asp Tyr Glu Ala
            20                  25                  30          
Ile Arg Arg Ala Ser Ile Ser Glu Ile Ser Glu Ala Ile Lys Glu Arg
        35                  40                  45              
Gly Met Asn Asn Met Leu Ala Val Arg Ile Lys Asp Phe Leu Glu Arg
    50                  55                  60                  
Ile Val Lys Asp His Gly Gly Ile Asp Leu Glu Trp Leu Arg Glu Ser
65                  70                  75                  80  
Pro Pro Asp Lys Ala Lys Asp Tyr Leu Leu Ser Ile Arg Gly Leu Gly
                85                  90                  95      
Leu Lys Ser Val Glu Cys Val Arg Leu Leu Thr Leu His Asn Leu Ala
            100                 105                 110         
Phe Pro Val Asp Thr Asn Val Gly Arg Ile Ala Val Arg Met Gly Trp
        115                 120                 125             
Val Pro Leu Gln Pro Leu Pro Glu Ser Leu Gln Leu His Leu Leu Glu
    130                 135                 140                 
Leu Tyr Pro Val Leu Glu Ser Ile Gln Lys Phe Leu Trp Pro Arg Leu
145                 150                 155                 160 
Cys Lys Leu Asp Gln Arg Thr Leu Tyr Glu Leu His Tyr Gln Leu Ile
                165                 170                 175     
Thr Phe Gly Lys Val Phe Cys Thr Lys Ser Arg Pro Asn Cys Asn Ala
            180                 185                 190         
Cys Pro Met Arg Gly Glu Cys Arg His Phe Ala Ser Ala Tyr Ala Ser
        195                 200                 205             
Ala Arg Leu Ala Leu Pro Ala Pro Glu Glu Arg Ser Leu Thr Ser Ala
    210                 215                 220                 
Thr Ile Pro Val Pro Pro Glu Ser Phe Pro Pro Val Ala Ile Pro Met
225                 230                 235                 240 
Ile Glu Leu Pro Leu Pro Leu Glu Lys Ser Leu Ala Ser Gly Ala Pro
                245                 250                 255     
Ser Asn Arg Glu Asn Cys Glu Pro Ile Ile Glu Glu Pro Ala Ser Pro
            260                 265                 270         
Gly Gln Glu Cys Thr Glu Ile Thr Glu Ser Asp Ile Glu Asp Ala Tyr
        275                 280                 285             
Tyr Asn Glu Asp Pro Asp Glu Ile Pro Thr Ile Lys Leu Asn Ile Glu
    290                 295                 300                 
Gln Phe Gly Met Thr Leu Arg Glu His Met Glu Arg Asn Met Glu Leu
305                 310                 315                 320 
Gln Glu Gly Asp Met Ser Lys Ala Leu Val Ala Leu His Pro Thr Thr
                325                 330                 335     
Thr Ser Ile Pro Thr Pro Lys Leu Lys Asn Ile Ser Arg Leu Arg Thr
            340                 345                 350         
Glu His Gln Val Tyr Glu Leu Pro Asp Ser His Arg Leu Leu Asp Gly
        355                 360                 365             
Met Asp Lys Arg Glu Pro Asp Asp Pro Ser Pro Tyr Leu Leu Ala Ile
    370                 375                 380                 
Trp Thr Pro Gly Glu Thr Ala Asn Ser Ala Gln Pro Pro Glu Gln Lys
385                 390                 395                 400 
Cys Gly Gly Lys Ala Ser Gly Lys Met Cys Phe Asp Glu Thr Cys Ser
                405                 410                 415     
Glu Cys Asn Ser Leu Arg Glu Ala Asn Ser Gln Thr Val Arg Gly Thr
            420                 425                 430         
Leu Leu Ile Pro Cys Arg Thr Ala Met Arg Gly Ser Phe Pro Leu Asn
        435                 440                 445             
Gly Thr Tyr Phe Gln Val Asn Glu Leu Phe Ala Asp His Glu Ser Ser
    450                 455                 460                 
Leu Lys Pro Ile Asp Val Pro Arg Asp Trp Ile Trp Asp Leu Pro Arg
465                 470                 475                 480 
Arg Thr Val Tyr Phe Gly Thr Ser Val Thr Ser Ile Phe Arg Gly Leu
                485                 490                 495     
Ser Thr Glu Gln Ile Gln Phe Cys Phe Trp Lys Gly Phe Val Cys Val
            500                 505                 510         
Arg Gly Phe Glu Gln Lys Thr Arg Ala Pro Arg Pro Leu Met Ala Arg
        515                 520                 525             
Leu His Phe Pro Ala Ser Lys Leu Lys Asn Asn Lys Thr
    530                 535                 540     


<210> 3
<211> 391
<212> PRT
<213> Arabidopsis thaliana

<220> 
<223> positions 798 to 1188 of DME gene

<400> 3
Lys Leu Ser Ser Ser Arg Glu Asp Glu Arg Asn Val Arg Ser Val Val
 1               5                  10                  15      
Val Glu Asp Pro Glu Gly Cys Ile Leu Asn Leu Asn Glu Ile Pro Ser
            20                  25                  30          
Trp Gln Glu Lys Val Gln His Pro Ser Asp Met Glu Val Ser Gly Val
        35                  40                  45              
Asp Ser Gly Ser Lys Glu Gln Leu Arg Asp Cys Ser Asn Ser Gly Ile
    50                  55                  60                  
Glu Arg Phe Asn Phe Leu Glu Lys Ser Ile Gln Asn Leu Glu Glu Glu
65                  70                  75                  80  
Val Leu Ser Ser Gln Asp Ser Phe Asp Pro Ala Ile Phe Gln Ser Cys
                85                  90                  95      
Gly Arg Val Gly Ser Cys Ser Cys Ser Lys Ser Asp Ala Glu Phe Pro
            100                 105                 110         
Thr Thr Arg Cys Glu Thr Lys Thr Val Ser Gly Thr Ser Gln Ser Val
        115                 120                 125             
Gln Thr Gly Ser Pro Asn Leu Ser Asp Glu Ile Cys Leu Gln Gly Asn
    130                 135                 140                 
Glu Arg Pro His Leu Tyr Glu Gly Ser Gly Asp Val Gln Lys Gln Glu
145                 150                 155                 160 
Thr Thr Asn Val Ala Gln Lys Lys Pro Asp Leu Glu Lys Thr Met Asn
                165                 170                 175     
Trp Lys Asp Ser Val Cys Phe Gly Gln Pro Arg Asn Asp Thr Asn Trp
            180                 185                 190         
Gln Thr Thr Pro Ser Ser Ser Tyr Glu Gln Cys Ala Thr Arg Gln Pro
        195                 200                 205             
His Val Leu Asp Ile Glu Asp Phe Gly Met Gln Gly Glu Gly Leu Gly
    210                 215                 220                 
Tyr Ser Trp Met Ser Ile Ser Pro Arg Val Asp Arg Val Lys Asn Lys
225                 230                 235                 240 
Asn Val Pro Arg Arg Phe Phe Arg Gln Gly Gly Ser Val Pro Arg Glu
                245                 250                 255     
Phe Thr Gly Gln Ile Ile Pro Ser Thr Pro His Glu Leu Pro Gly Met
            260                 265                 270         
Gly Leu Ser Gly Ser Ser Ser Ala Val Gln Glu His Gln Asp Asp Thr
        275                 280                 285             
Gln His Asn Gln Gln Asp Glu Met Asn Lys Ala Ser His Leu Gln Lys
    290                 295                 300                 
Thr Phe Leu Asp Leu Leu Asn Ser Ser Glu Glu Cys Leu Thr Arg Gln
305                 310                 315                 320 
Ser Ser Thr Lys Gln Asn Ile Thr Asp Gly Cys Leu Pro Arg Asp Arg
                325                 330                 335     
Thr Ala Glu Asp Val Val Asp Pro Leu Ser Asn Asn Ser Ser Leu Gln
            340                 345                 350         
Asn Ile Leu Val Glu Ser Asn Ser Ser Asn Lys Glu Gln Thr Ala Val
        355                 360                 365             
Glu Tyr Lys Glu Thr Asn Ala Thr Ile Leu Arg Glu Met Lys Gly Thr
    370                 375                 380                 
Leu Ala Asp Gly Lys Lys Pro
385                 390     


<210> 4
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic amino acid linker lnk

<400> 4
Ala Gly Ser Ser Gly Asn Gly Ser Ser Gly Asn Gly
 1               5                  10          


<210> 5
<211> 1729
<212> PRT
<213> Arabidopsis thaliana

<220> 
<223> amino acid sequence of gene DME

<400> 5
Met Gln Ser Ile Met Asp Ser Ser Ala Val Asn Ala Thr Glu Ala Thr
 1               5                  10                  15      
Glu Gln Asn Asp Gly Ser Arg Gln Asp Val Leu Glu Phe Asp Leu Asn
            20                  25                  30          
Lys Thr Pro Gln Gln Lys Pro Ser Lys Arg Lys Arg Lys Phe Met Pro
        35                  40                  45              
Lys Val Val Val Glu Gly Lys Pro Lys Arg Lys Pro Arg Lys Pro Ala
    50                  55                  60                  
Glu Leu Pro Lys Val Val Val Glu Gly Lys Pro Lys Arg Lys Pro Arg
65                  70                  75                  80  
Lys Ala Ala Thr Gln Glu Lys Val Lys Ser Lys Glu Thr Gly Ser Ala
                85                  90                  95      
Lys Lys Lys Asn Leu Lys Glu Ser Ala Thr Lys Lys Pro Ala Asn Val
            100                 105                 110         
Gly Asp Met Ser Asn Lys Ser Pro Glu Val Thr Leu Lys Ser Cys Arg
        115                 120                 125             
Lys Ala Leu Asn Phe Asp Leu Glu Asn Pro Gly Asp Ala Arg Gln Gly
    130                 135                 140                 
Asp Ser Glu Ser Glu Ile Val Gln Asn Ser Ser Gly Ala Asn Ser Phe
145                 150                 155                 160 
Ser Glu Ile Arg Asp Ala Ile Gly Gly Thr Asn Gly Ser Phe Leu Asp
                165                 170                 175     
Ser Val Ser Gln Ile Asp Lys Thr Asn Gly Leu Gly Ala Met Asn Gln
            180                 185                 190         
Pro Leu Glu Val Ser Met Gly Asn Gln Pro Asp Lys Leu Ser Thr Gly
        195                 200                 205             
Ala Lys Leu Ala Arg Asp Gln Gln Pro Asp Leu Leu Thr Arg Asn Gln
    210                 215                 220                 
Gln Cys Gln Phe Pro Val Ala Thr Gln Asn Thr Gln Phe Pro Met Glu
225                 230                 235                 240 
Asn Gln Gln Ala Trp Leu Gln Met Lys Asn Gln Leu Ile Gly Phe Pro
                245                 250                 255     
Phe Gly Asn Gln Gln Pro Arg Met Thr Ile Arg Asn Gln Gln Pro Cys
            260                 265                 270         
Leu Ala Met Gly Asn Gln Gln Pro Met Tyr Leu Ile Gly Thr Pro Arg
        275                 280                 285             
Pro Ala Leu Val Ser Gly Asn Gln Gln Leu Gly Gly Pro Gln Gly Asn
    290                 295                 300                 
Lys Arg Pro Ile Phe Leu Asn His Gln Thr Cys Leu Pro Ala Gly Asn
305                 310                 315                 320 
Gln Leu Tyr Gly Ser Pro Thr Asp Met His Gln Leu Val Met Ser Thr
                325                 330                 335     
Gly Gly Gln Gln His Gly Leu Leu Ile Lys Asn Gln Gln Pro Gly Ser
            340                 345                 350         
Leu Ile Arg Gly Gln Gln Pro Cys Val Pro Leu Ile Asp Gln Gln Pro
        355                 360                 365             
Ala Thr Pro Lys Gly Phe Thr His Leu Asn Gln Met Val Ala Thr Ser
    370                 375                 380                 
Met Ser Ser Pro Gly Leu Arg Pro His Ser Gln Ser Gln Val Pro Thr
385                 390                 395                 400 
Thr Tyr Leu His Val Glu Ser Val Ser Arg Ile Leu Asn Gly Thr Thr
                405                 410                 415     
Gly Thr Cys Gln Arg Ser Arg Ala Pro Ala Tyr Asp Ser Leu Gln Gln
            420                 425                 430         
Asp Ile His Gln Gly Asn Lys Tyr Ile Leu Ser His Glu Ile Ser Asn
        435                 440                 445             
Gly Asn Gly Cys Lys Lys Ala Leu Pro Gln Asn Ser Ser Leu Pro Thr
    450                 455                 460                 
Pro Ile Met Ala Lys Leu Glu Glu Ala Arg Gly Ser Lys Arg Gln Tyr
465                 470                 475                 480 
His Arg Ala Met Gly Gln Thr Glu Lys His Asp Leu Asn Leu Ala Gln
                485                 490                 495     
Gln Ile Ala Gln Ser Gln Asp Val Glu Arg His Asn Ser Ser Thr Cys
            500                 505                 510         
Val Glu Tyr Leu Asp Ala Ala Lys Lys Thr Lys Ile Gln Lys Val Val
        515                 520                 525             
Gln Glu Asn Leu His Gly Met Pro Pro Glu Val Ile Glu Ile Glu Asp
    530                 535                 540                 
Asp Pro Thr Asp Gly Ala Arg Lys Gly Lys Asn Thr Ala Ser Ile Ser
545                 550                 555                 560 
Lys Gly Ala Ser Lys Gly Asn Ser Ser Pro Val Lys Lys Thr Ala Glu
                565                 570                 575     
Lys Glu Lys Cys Ile Val Pro Lys Thr Pro Ala Lys Lys Gly Arg Ala
            580                 585                 590         
Gly Arg Lys Lys Ser Val Pro Pro Pro Ala His Ala Ser Glu Ile Gln
        595                 600                 605             
Leu Trp Gln Pro Thr Pro Pro Lys Thr Pro Leu Ser Arg Ser Lys Pro
    610                 615                 620                 
Lys Gly Lys Gly Arg Lys Ser Ile Gln Asp Ser Gly Lys Ala Arg Gly
625                 630                 635                 640 
Pro Ser Gly Glu Leu Leu Cys Gln Asp Ser Ile Ala Glu Ile Ile Tyr
                645                 650                 655     
Arg Met Gln Asn Leu Tyr Leu Gly Asp Lys Glu Arg Glu Gln Glu Gln
            660                 665                 670         
Asn Ala Met Val Leu Tyr Lys Gly Asp Gly Ala Leu Val Pro Tyr Glu
        675                 680                 685             
Ser Lys Lys Arg Lys Pro Arg Pro Lys Val Asp Ile Asp Asp Glu Thr
    690                 695                 700                 
Thr Arg Ile Trp Asn Leu Leu Met Gly Lys Gly Asp Glu Lys Glu Gly
705                 710                 715                 720 
Asp Glu Glu Lys Asp Lys Lys Lys Glu Lys Trp Trp Glu Glu Glu Arg
                725                 730                 735     
Arg Val Phe Arg Gly Arg Ala Asp Ser Phe Ile Ala Arg Met His Leu
            740                 745                 750         
Val Gln Gly Asp Arg Arg Phe Ser Pro Trp Lys Gly Ser Val Val Asp
        755                 760                 765             
Ser Val Ile Gly Val Phe Leu Thr Gln Asn Val Ser Asp His Leu Ser
    770                 775                 780                 
Ser Ser Ala Phe Met Ser Leu Ala Ala Arg Phe Pro Pro Lys Leu Ser
785                 790                 795                 800 
Ser Ser Arg Glu Asp Glu Arg Asn Val Arg Ser Val Val Val Glu Asp
                805                 810                 815     
Pro Glu Gly Cys Ile Leu Asn Leu Asn Glu Ile Pro Ser Trp Gln Glu
            820                 825                 830         
Lys Val Gln His Pro Ser Asp Met Glu Val Ser Gly Val Asp Ser Gly
        835                 840                 845             
Ser Lys Glu Gln Leu Arg Asp Cys Ser Asn Ser Gly Ile Glu Arg Phe
    850                 855                 860                 
Asn Phe Leu Glu Lys Ser Ile Gln Asn Leu Glu Glu Glu Val Leu Ser
865                 870                 875                 880 
Ser Gln Asp Ser Phe Asp Pro Ala Ile Phe Gln Ser Cys Gly Arg Val
                885                 890                 895     
Gly Ser Cys Ser Cys Ser Lys Ser Asp Ala Glu Phe Pro Thr Thr Arg
            900                 905                 910         
Cys Glu Thr Lys Thr Val Ser Gly Thr Ser Gln Ser Val Gln Thr Gly
        915                 920                 925             
Ser Pro Asn Leu Ser Asp Glu Ile Cys Leu Gln Gly Asn Glu Arg Pro
    930                 935                 940                 
His Leu Tyr Glu Gly Ser Gly Asp Val Gln Lys Gln Glu Thr Thr Asn
945                 950                 955                 960 
Val Ala Gln Lys Lys Pro Asp Leu Glu Lys Thr Met Asn Trp Lys Asp
                965                 970                 975     
Ser Val Cys Phe Gly Gln Pro Arg Asn Asp Thr Asn Trp Gln Thr Thr
            980                 985                 990         
Pro Ser Ser Ser Tyr Glu Gln Cys Ala Thr Arg Gln Pro His Val Leu
        995                 1000                1005            
Asp Ile Glu Asp Phe Gly Met Gln Gly Glu Gly Leu Gly Tyr Ser Trp
    1010                1015                1020                
Met Ser Ile Ser Pro Arg Val Asp Arg Val Lys Asn Lys Asn Val Pro
1025                1030                1035                1040
Arg Arg Phe Phe Arg Gln Gly Gly Ser Val Pro Arg Glu Phe Thr Gly
                1045                1050                1055    
Gln Ile Ile Pro Ser Thr Pro His Glu Leu Pro Gly Met Gly Leu Ser
            1060                1065                1070        
Gly Ser Ser Ser Ala Val Gln Glu His Gln Asp Asp Thr Gln His Asn
        1075                1080                1085            
Gln Gln Asp Glu Met Asn Lys Ala Ser His Leu Gln Lys Thr Phe Leu
    1090                1095                1100                
Asp Leu Leu Asn Ser Ser Glu Glu Cys Leu Thr Arg Gln Ser Ser Thr
1105                1110                1115                1120
Lys Gln Asn Ile Thr Asp Gly Cys Leu Pro Arg Asp Arg Thr Ala Glu
                1125                1130                1135    
Asp Val Val Asp Pro Leu Ser Asn Asn Ser Ser Leu Gln Asn Ile Leu
            1140                1145                1150        
Val Glu Ser Asn Ser Ser Asn Lys Glu Gln Thr Ala Val Glu Tyr Lys
        1155                1160                1165            
Glu Thr Asn Ala Thr Ile Leu Arg Glu Met Lys Gly Thr Leu Ala Asp
    1170                1175                1180                
Gly Lys Lys Pro Thr Ser Gln Trp Asp Ser Leu Arg Lys Asp Val Glu
1185                1190                1195                1200
Gly Asn Glu Gly Arg Gln Glu Arg Asn Lys Asn Asn Met Asp Ser Ile
                1205                1210                1215    
Asp Tyr Glu Ala Ile Arg Arg Ala Ser Ile Ser Glu Ile Ser Glu Ala
            1220                1225                1230        
Ile Lys Glu Arg Gly Met Asn Asn Met Leu Ala Val Arg Ile Lys Asp
        1235                1240                1245            
Phe Leu Glu Arg Ile Val Lys Asp His Gly Gly Ile Asp Leu Glu Trp
    1250                1255                1260                
Leu Arg Glu Ser Pro Pro Asp Lys Ala Lys Asp Tyr Leu Leu Ser Ile
1265                1270                1275                1280
Arg Gly Leu Gly Leu Lys Ser Val Glu Cys Val Arg Leu Leu Thr Leu
                1285                1290                1295    
His Asn Leu Ala Phe Pro Val Asp Thr Asn Val Gly Arg Ile Ala Val
            1300                1305                1310        
Arg Met Gly Trp Val Pro Leu Gln Pro Leu Pro Glu Ser Leu Gln Leu
        1315                1320                1325            
His Leu Leu Glu Leu Tyr Pro Val Leu Glu Ser Ile Gln Lys Phe Leu
    1330                1335                1340                
Trp Pro Arg Leu Cys Lys Leu Asp Gln Arg Thr Leu Tyr Glu Leu His
1345                1350                1355                1360
Tyr Gln Leu Ile Thr Phe Gly Lys Val Phe Cys Thr Lys Ser Arg Pro
                1365                1370                1375    
Asn Cys Asn Ala Cys Pro Met Arg Gly Glu Cys Arg His Phe Ala Ser
            1380                1385                1390        
Ala Tyr Ala Ser Ala Arg Leu Ala Leu Pro Ala Pro Glu Glu Arg Ser
        1395                1400                1405            
Leu Thr Ser Ala Thr Ile Pro Val Pro Pro Glu Ser Phe Pro Pro Val
    1410                1415                1420                
Ala Ile Pro Met Ile Glu Leu Pro Leu Pro Leu Glu Lys Ser Leu Ala
1425                1430                1435                1440
Ser Gly Ala Pro Ser Asn Arg Glu Asn Cys Glu Pro Ile Ile Glu Glu
                1445                1450                1455    
Pro Ala Ser Pro Gly Gln Glu Cys Thr Glu Ile Thr Glu Ser Asp Ile
            1460                1465                1470        
Glu Asp Ala Tyr Tyr Asn Glu Asp Pro Asp Glu Ile Pro Thr Ile Lys
        1475                1480                1485            
Leu Asn Ile Glu Gln Phe Gly Met Thr Leu Arg Glu His Met Glu Arg
    1490                1495                1500                
Asn Met Glu Leu Gln Glu Gly Asp Met Ser Lys Ala Leu Val Ala Leu
1505                1510                1515                1520
His Pro Thr Thr Thr Ser Ile Pro Thr Pro Lys Leu Lys Asn Ile Ser
                1525                1530                1535    
Arg Leu Arg Thr Glu His Gln Val Tyr Glu Leu Pro Asp Ser His Arg
            1540                1545                1550        
Leu Leu Asp Gly Met Asp Lys Arg Glu Pro Asp Asp Pro Ser Pro Tyr
        1555                1560                1565            
Leu Leu Ala Ile Trp Thr Pro Gly Glu Thr Ala Asn Ser Ala Gln Pro
    1570                1575                1580                
Pro Glu Gln Lys Cys Gly Gly Lys Ala Ser Gly Lys Met Cys Phe Asp
1585                1590                1595                1600
Glu Thr Cys Ser Glu Cys Asn Ser Leu Arg Glu Ala Asn Ser Gln Thr
                1605                1610                1615    
Val Arg Gly Thr Leu Leu Ile Pro Cys Arg Thr Ala Met Arg Gly Ser
            1620                1625                1630        
Phe Pro Leu Asn Gly Thr Tyr Phe Gln Val Asn Glu Leu Phe Ala Asp
        1635                1640                1645            
His Glu Ser Ser Leu Lys Pro Ile Asp Val Pro Arg Asp Trp Ile Trp
    1650                1655                1660                
Asp Leu Pro Arg Arg Thr Val Tyr Phe Gly Thr Ser Val Thr Ser Ile
1665                1670                1675                1680
Phe Arg Gly Leu Ser Thr Glu Gln Ile Gln Phe Cys Phe Trp Lys Gly
                1685                1690                1695    
Phe Val Cys Val Arg Gly Phe Glu Gln Lys Thr Arg Ala Pro Arg Pro
            1700                1705                1710        
Leu Met Ala Arg Leu His Phe Pro Ala Ser Lys Leu Lys Asn Asn Lys
        1715                1720                1725            
Thr
    


<210> 6
<211> 1393
<212> PRT
<213> Arabidopsis thaliana

<220> 
<223> amino acid sequence of ROS1 gene

<400> 6
Met Glu Lys Gln Arg Arg Glu Glu Ser Ser Phe Gln Gln Pro Pro Trp
 1               5                  10                  15      
Ile Pro Gln Thr Pro Met Lys Pro Phe Ser Pro Ile Cys Pro Tyr Thr
            20                  25                  30          
Val Glu Asp Gln Tyr His Ser Ser Gln Leu Glu Glu Arg Arg Phe Val
        35                  40                  45              
Gly Asn Lys Asp Met Ser Gly Leu Asp His Leu Ser Phe Gly Asp Leu
    50                  55                  60                  
Leu Ala Leu Ala Asn Thr Ala Ser Leu Ile Phe Ser Gly Gln Thr Pro
65                  70                  75                  80  
Ile Pro Thr Arg Asn Thr Glu Val Met Gln Lys Gly Thr Glu Glu Val
                85                  90                  95      
Glu Ser Leu Ser Ser Val Ser Asn Asn Val Ala Glu Gln Ile Leu Lys
            100                 105                 110         
Thr Pro Glu Lys Pro Lys Arg Lys Lys His Arg Pro Lys Val Arg Arg
        115                 120                 125             
Glu Ala Lys Pro Lys Arg Glu Pro Lys Pro Arg Ala Pro Arg Lys Ser
    130                 135                 140                 
Val Val Thr Asp Gly Gln Glu Ser Lys Thr Pro Lys Arg Lys Tyr Val
145                 150                 155                 160 
Arg Lys Lys Val Glu Val Ser Lys Asp Gln Asp Ala Thr Pro Val Glu
                165                 170                 175     
Ser Ser Ala Ala Val Glu Thr Ser Thr Arg Pro Lys Arg Leu Cys Arg
            180                 185                 190         
Arg Val Leu Asp Phe Glu Ala Glu Asn Gly Glu Asn Gln Thr Asn Gly
        195                 200                 205             
Asp Ile Arg Glu Ala Gly Glu Met Glu Ser Ala Leu Gln Glu Lys Gln
    210                 215                 220                 
Leu Asp Ser Gly Asn Gln Glu Leu Lys Asp Cys Leu Leu Ser Ala Pro
225                 230                 235                 240 
Ser Thr Pro Lys Arg Lys Arg Ser Gln Gly Lys Arg Lys Gly Val Gln
                245                 250                 255     
Pro Lys Lys Asn Gly Ser Asn Leu Glu Glu Val Asp Ile Ser Met Ala
            260                 265                 270         
Gln Ala Ala Lys Arg Arg Gln Gly Pro Thr Cys Cys Asp Met Asn Leu
        275                 280                 285             
Ser Gly Ile Gln Tyr Asp Glu Gln Cys Asp Tyr Gln Lys Met His Trp
    290                 295                 300                 
Leu Tyr Ser Pro Asn Leu Gln Gln Gly Gly Met Arg Tyr Asp Ala Ile
305                 310                 315                 320 
Cys Ser Lys Val Phe Ser Gly Gln Gln His Asn Tyr Val Ser Ala Phe
                325                 330                 335     
His Ala Thr Cys Tyr Ser Ser Thr Ser Gln Leu Ser Ala Asn Arg Val
            340                 345                 350         
Leu Thr Val Glu Glu Arg Arg Glu Gly Ile Phe Gln Gly Arg Gln Glu
        355                 360                 365             
Ser Glu Leu Asn Val Leu Ser Asp Lys Ile Asp Thr Pro Ile Lys Lys
    370                 375                 380                 
Lys Thr Thr Gly His Ala Arg Phe Arg Asn Leu Ser Ser Met Asn Lys
385                 390                 395                 400 
Leu Val Glu Val Pro Glu His Leu Thr Ser Gly Tyr Cys Ser Lys Pro
                405                 410                 415     
Gln Gln Asn Asn Lys Ile Leu Val Asp Thr Arg Val Thr Val Ser Lys
            420                 425                 430         
Lys Lys Pro Thr Lys Ser Glu Lys Ser Gln Thr Lys Gln Lys Asn Leu
        435                 440                 445             
Leu Pro Asn Leu Cys Arg Phe Pro Pro Ser Phe Thr Gly Leu Ser Pro
    450                 455                 460                 
Asp Glu Leu Trp Lys Arg Arg Asn Ser Ile Glu Thr Ile Ser Glu Leu
465                 470                 475                 480 
Leu Arg Leu Leu Asp Ile Asn Arg Glu His Ser Glu Thr Ala Leu Val
                485                 490                 495     
Pro Tyr Thr Met Asn Ser Gln Ile Val Leu Phe Gly Gly Gly Ala Gly
            500                 505                 510         
Ala Ile Val Pro Val Thr Pro Val Lys Lys Pro Arg Pro Arg Pro Lys
        515                 520                 525             
Val Asp Leu Asp Asp Glu Thr Asp Arg Val Trp Lys Leu Leu Leu Glu
    530                 535                 540                 
Asn Ile Asn Ser Glu Gly Val Asp Gly Ser Asp Glu Gln Lys Ala Lys
545                 550                 555                 560 
Trp Trp Glu Glu Glu Arg Asn Val Phe Arg Gly Arg Ala Asp Ser Phe
                565                 570                 575     
Ile Ala Arg Met His Leu Val Gln Gly Asp Arg Arg Phe Thr Pro Trp
            580                 585                 590         
Lys Gly Ser Val Val Asp Ser Val Val Gly Val Phe Leu Thr Gln Asn
        595                 600                 605             
Val Ser Asp His Leu Ser Ser Ser Ala Phe Met Ser Leu Ala Ser Gln
    610                 615                 620                 
Phe Pro Val Pro Phe Val Pro Ser Ser Asn Phe Asp Ala Gly Thr Ser
625                 630                 635                 640 
Ser Met Pro Ser Ile Gln Ile Thr Tyr Leu Asp Ser Glu Glu Thr Met
                645                 650                 655     
Ser Ser Pro Pro Asp His Asn His Ser Ser Val Thr Leu Lys Asn Thr
            660                 665                 670         
Gln Pro Asp Glu Glu Lys Asp Tyr Val Pro Ser Asn Glu Thr Ser Arg
        675                 680                 685             
Ser Ser Ser Glu Ile Ala Ile Ser Ala His Glu Ser Val Asp Lys Thr
    690                 695                 700                 
Thr Asp Ser Lys Glu Tyr Val Asp Ser Asp Arg Lys Gly Ser Ser Val
705                 710                 715                 720 
Glu Val Asp Lys Thr Asp Glu Lys Cys Arg Val Leu Asn Leu Phe Pro
                725                 730                 735     
Ser Glu Asp Ser Ala Leu Thr Cys Gln His Ser Met Val Ser Asp Ala
            740                 745                 750         
Pro Gln Asn Thr Glu Arg Ala Gly Ser Ser Ser Glu Ile Asp Leu Glu
        755                 760                 765             
Gly Glu Tyr Arg Thr Ser Phe Met Lys Leu Leu Gln Gly Val Gln Val
    770                 775                 780                 
Ser Leu Glu Asp Ser Asn Gln Val Ser Pro Asn Met Ser Pro Gly Asp
785                 790                 795                 800 
Cys Ser Ser Glu Ile Lys Gly Phe Gln Ser Met Lys Glu Pro Thr Lys
                805                 810                 815     
Ser Ser Val Asp Ser Ser Glu Pro Gly Cys Cys Ser Gln Gln Asp Gly
            820                 825                 830         
Asp Val Leu Ser Cys Gln Lys Pro Thr Leu Lys Glu Lys Gly Lys Lys
        835                 840                 845             
Val Leu Lys Glu Glu Lys Lys Ala Phe Asp Trp Asp Cys Leu Arg Arg
    850                 855                 860                 
Glu Ala Gln Ala Arg Ala Gly Ile Arg Glu Lys Thr Arg Ser Thr Met
865                 870                 875                 880 
Asp Thr Val Asp Trp Lys Ala Ile Arg Ala Ala Asp Val Lys Glu Val
                885                 890                 895     
Ala Glu Thr Ile Lys Ser Arg Gly Met Asn His Lys Leu Ala Glu Arg
            900                 905                 910         
Ile Gln Gly Phe Leu Asp Arg Leu Val Asn Asp His Gly Ser Ile Asp
        915                 920                 925             
Leu Glu Trp Leu Arg Asp Val Pro Pro Asp Lys Ala Lys Glu Tyr Leu
    930                 935                 940                 
Leu Ser Phe Asn Gly Leu Gly Leu Lys Ser Val Glu Cys Val Arg Leu
945                 950                 955                 960 
Leu Thr Leu His His Leu Ala Phe Pro Val Asp Thr Asn Val Gly Arg
                965                 970                 975     
Ile Ala Val Arg Leu Gly Trp Val Pro Leu Gln Pro Leu Pro Glu Ser
            980                 985                 990         
Leu Gln Leu His Leu Leu Glu Met Tyr Pro Met Leu Glu Ser Ile Gln
        995                 1000                1005            
Lys Tyr Leu Trp Pro Arg Leu Cys Lys Leu Asp Gln Lys Thr Leu Tyr
    1010                1015                1020                
Glu Leu His Tyr Gln Met Ile Thr Phe Gly Lys Val Phe Cys Thr Lys
1025                1030                1035                1040
Ser Lys Pro Asn Cys Asn Ala Cys Pro Met Lys Gly Glu Cys Arg His
                1045                1050                1055    
Phe Ala Ser Ala Phe Ala Ser Ala Arg Leu Ala Leu Pro Ser Thr Glu
            1060                1065                1070        
Lys Gly Met Gly Thr Pro Asp Lys Asn Pro Leu Pro Leu His Leu Pro
        1075                1080                1085            
Glu Pro Phe Gln Arg Glu Gln Gly Ser Glu Val Val Gln His Ser Glu
    1090                1095                1100                
Pro Ala Lys Lys Val Thr Cys Cys Glu Pro Ile Ile Glu Glu Pro Ala
1105                1110                1115                1120
Ser Pro Glu Pro Glu Thr Ala Glu Val Ser Ile Ala Asp Ile Glu Glu
                1125                1130                1135    
Ala Phe Phe Glu Asp Pro Glu Glu Ile Pro Thr Ile Arg Leu Asn Met
            1140                1145                1150        
Asp Ala Phe Thr Ser Asn Leu Lys Lys Ile Met Glu His Asn Lys Glu
        1155                1160                1165            
Leu Gln Asp Gly Asn Met Ser Ser Ala Leu Val Ala Leu Thr Ala Glu
    1170                1175                1180                
Thr Ala Ser Leu Pro Met Pro Lys Leu Lys Asn Ile Ser Gln Leu Arg
1185                1190                1195                1200
Thr Glu His Arg Val Tyr Glu Leu Pro Asp Glu His Pro Leu Leu Ala
                1205                1210                1215    
Gln Leu Glu Lys Arg Glu Pro Asp Asp Pro Cys Ser Tyr Leu Leu Ala
            1220                1225                1230        
Ile Trp Thr Pro Gly Glu Thr Ala Asp Ser Ile Gln Pro Ser Val Ser
        1235                1240                1245            
Thr Cys Ile Phe Gln Ala Asn Gly Met Leu Cys Asp Glu Glu Thr Cys
    1250                1255                1260                
Phe Ser Cys Asn Ser Ile Lys Glu Thr Arg Ser Gln Ile Val Arg Gly
1265                1270                1275                1280
Thr Ile Leu Ile Pro Cys Arg Thr Ala Met Arg Gly Ser Phe Pro Leu
                1285                1290                1295    
Asn Gly Thr Tyr Phe Gln Val Asn Glu Val Phe Ala Asp His Ala Ser
            1300                1305                1310        
Ser Leu Asn Pro Ile Asn Val Pro Arg Glu Leu Ile Trp Glu Leu Pro
        1315                1320                1325            
Arg Arg Thr Val Tyr Phe Gly Thr Ser Val Pro Thr Ile Phe Lys Gly
    1330                1335                1340                
Leu Ser Thr Glu Lys Ile Gln Ala Cys Phe Trp Lys Gly Tyr Val Cys
1345                1350                1355                1360
Val Arg Gly Phe Asp Arg Lys Thr Arg Gly Pro Lys Pro Leu Ile Ala
                1365                1370                1375    
Arg Leu His Phe Pro Ala Ser Lys Leu Lys Gly Gln Gln Ala Asn Leu
            1380                1385                1390        
Ala
    


<210> 7
<211> 1332
<212> PRT
<213> Arabidopsis thaliana

<220> 
<223> amino acid sequence of DML2 gene

<400> 7
Met Glu Val Glu Gly Glu Val Arg Glu Lys Glu Ala Arg Val Lys Gly
 1               5                  10                  15      
Arg Gln Pro Glu Thr Glu Val Leu His Gly Leu Pro Gln Glu Gln Ser
            20                  25                  30          
Ile Phe Asn Asn Met Gln His Asn His Gln Pro Asp Ser Asp Arg Arg
        35                  40                  45              
Arg Leu Ser Leu Glu Asn Leu Pro Gly Leu Tyr Asn Met Ser Cys Thr
    50                  55                  60                  
Gln Leu Leu Ala Leu Ala Asn Ala Thr Val Ala Thr Gly Ser Ser Ile
65                  70                  75                  80  
Gly Ala Ser Ser Ser Ser Leu Ser Ser Gln His Pro Thr Asp Ser Trp
                85                  90                  95      
Ile Asn Ser Trp Lys Met Asp Ser Asn Pro Trp Thr Leu Ser Lys Met
            100                 105                 110         
Gln Lys Gln Gln Tyr Asp Val Ser Thr Pro Gln Lys Phe Leu Cys Asp
        115                 120                 125             
Leu Asn Leu Thr Pro Glu Glu Leu Val Ser Thr Ser Thr Gln Arg Thr
    130                 135                 140                 
Glu Pro Glu Ser Pro Gln Ile Thr Leu Lys Thr Pro Gly Lys Ser Leu
145                 150                 155                 160 
Ser Glu Thr Asp His Glu Pro His Asp Arg Ile Lys Lys Ser Val Leu
                165                 170                 175     
Gly Thr Gly Ser Pro Ala Ala Val Lys Lys Arg Lys Ile Ala Arg Asn
            180                 185                 190         
Asp Glu Lys Ser Gln Leu Glu Thr Pro Thr Leu Lys Arg Lys Lys Ile
        195                 200                 205             
Arg Pro Lys Val Val Arg Glu Gly Lys Thr Lys Lys Ala Ser Ser Lys
    210                 215                 220                 
Ala Gly Ile Lys Lys Ser Ser Ile Ala Ala Thr Ala Thr Lys Thr Ser
225                 230                 235                 240 
Glu Glu Ser Asn Tyr Val Arg Pro Lys Arg Leu Thr Arg Arg Ser Ile
                245                 250                 255     
Arg Phe Asp Phe Asp Leu Gln Glu Glu Asp Glu Glu Phe Cys Gly Ile
            260                 265                 270         
Asp Phe Thr Ser Ala Gly His Val Glu Gly Ser Ser Gly Glu Glu Asn
        275                 280                 285             
Leu Thr Asp Thr Thr Leu Gly Met Phe Gly His Val Pro Lys Gly Arg
    290                 295                 300                 
Arg Gly Gln Arg Arg Ser Asn Gly Phe Lys Lys Thr Asp Asn Asp Cys
305                 310                 315                 320 
Leu Ser Ser Met Leu Ser Leu Val Asn Thr Gly Pro Gly Ser Phe Met
                325                 330                 335     
Glu Ser Glu Glu Asp Arg Pro Ser Asp Ser Gln Ile Ser Leu Gly Arg
            340                 345                 350         
Gln Arg Ser Ile Met Ala Thr Arg Pro Arg Asn Phe Arg Ser Leu Lys
        355                 360                 365             
Lys Leu Leu Gln Arg Ile Ile Pro Ser Lys Arg Asp Arg Lys Gly Cys
    370                 375                 380                 
Lys Leu Pro Arg Gly Leu Pro Lys Leu Thr Val Ala Ser Lys Leu Gln
385                 390                 395                 400 
Leu Lys Val Phe Arg Lys Lys Arg Ser Gln Arg Asn Arg Val Ala Ser
                405                 410                 415     
Gln Phe Asn Ala Arg Ile Leu Asp Leu Gln Trp Arg Arg Gln Asn Pro
            420                 425                 430         
Thr Gly Thr Ser Leu Ala Asp Ile Trp Glu Arg Ser Leu Thr Ile Asp
        435                 440                 445             
Ala Ile Thr Lys Leu Phe Glu Glu Leu Asp Ile Asn Lys Glu Gly Leu
    450                 455                 460                 
Cys Leu Pro His Asn Arg Glu Thr Ala Leu Ile Leu Tyr Lys Lys Ser
465                 470                 475                 480 
Tyr Glu Glu Gln Lys Ala Ile Val Lys Tyr Ser Lys Lys Gln Lys Pro
                485                 490                 495     
Lys Val Gln Leu Asp Pro Glu Thr Ser Arg Val Trp Lys Leu Leu Met
            500                 505                 510         
Ser Ser Ile Asp Cys Asp Gly Val Asp Gly Ser Asp Glu Glu Lys Arg
        515                 520                 525             
Lys Trp Trp Glu Glu Glu Arg Asn Met Phe His Gly Arg Ala Asn Ser
    530                 535                 540                 
Phe Ile Ala Arg Met Arg Val Val Gln Gly Asn Arg Thr Phe Ser Pro
545                 550                 555                 560 
Trp Lys Gly Ser Val Val Asp Ser Val Val Gly Val Phe Leu Thr Gln
                565                 570                 575     
Asn Val Ala Asp His Ser Ser Ser Ser Ala Tyr Met Asp Leu Ala Ala
            580                 585                 590         
Glu Phe Pro Val Glu Trp Asn Phe Asn Lys Gly Ser Cys His Glu Glu
        595                 600                 605             
Trp Gly Ser Ser Val Thr Gln Glu Thr Ile Leu Asn Leu Asp Pro Arg
    610                 615                 620                 
Thr Gly Val Ser Thr Pro Arg Ile Arg Asn Pro Thr Arg Val Ile Ile
625                 630                 635                 640 
Glu Glu Ile Asp Asp Asp Glu Asn Asp Ile Asp Ala Val Cys Ser Gln
                645                 650                 655     
Glu Ser Ser Lys Thr Ser Asp Ser Ser Ile Thr Ser Ala Asp Gln Ser
            660                 665                 670         
Lys Thr Met Leu Leu Asp Pro Phe Asn Thr Val Leu Met Asn Glu Gln
        675                 680                 685             
Val Asp Ser Gln Met Val Lys Gly Lys Gly His Ile Pro Tyr Thr Asp
    690                 695                 700                 
Asp Leu Asn Asp Leu Ser Gln Gly Ile Ser Met Val Ser Ser Ala Ser
705                 710                 715                 720 
Thr His Cys Glu Leu Asn Leu Asn Glu Val Pro Pro Glu Val Glu Leu
                725                 730                 735     
Cys Ser His Gln Gln Asp Pro Glu Ser Thr Ile Gln Thr Gln Asp Gln
            740                 745                 750         
Gln Glu Ser Thr Arg Thr Glu Asp Val Lys Lys Asn Arg Lys Lys Pro
        755                 760                 765             
Thr Thr Ser Lys Pro Lys Lys Lys Ser Lys Glu Ser Ala Lys Ser Thr
    770                 775                 780                 
Gln Lys Lys Ser Val Asp Trp Asp Ser Leu Arg Lys Glu Ala Glu Ser
785                 790                 795                 800 
Gly Gly Arg Lys Arg Glu Arg Thr Glu Arg Thr Met Asp Thr Val Asp
                805                 810                 815     
Trp Asp Ala Leu Arg Cys Thr Asp Val His Lys Ile Ala Asn Ile Ile
            820                 825                 830         
Ile Lys Arg Gly Met Asn Asn Met Leu Ala Glu Arg Ile Lys Ala Phe
        835                 840                 845             
Leu Asn Arg Leu Val Lys Lys His Gly Ser Ile Asp Leu Glu Trp Leu
    850                 855                 860                 
Arg Asp Val Pro Pro Asp Lys Ala Lys Glu Tyr Leu Leu Ser Ile Asn
865                 870                 875                 880 
Gly Leu Gly Leu Lys Ser Val Glu Cys Val Arg Leu Leu Ser Leu His
                885                 890                 895     
Gln Ile Ala Phe Pro Val Asp Thr Asn Val Gly Arg Ile Ala Val Arg
            900                 905                 910         
Leu Gly Trp Val Pro Leu Gln Pro Leu Pro Asp Glu Leu Gln Met His
        915                 920                 925             
Leu Leu Glu Leu Tyr Pro Val Leu Glu Ser Val Gln Lys Tyr Leu Trp
    930                 935                 940                 
Pro Arg Leu Cys Lys Leu Asp Gln Lys Thr Leu Tyr Glu Leu His Tyr
945                 950                 955                 960 
His Met Ile Thr Phe Gly Lys Val Phe Cys Thr Lys Val Lys Pro Asn
                965                 970                 975     
Cys Asn Ala Cys Pro Met Lys Ala Glu Cys Arg His Tyr Ser Ser Ala
            980                 985                 990         
Arg Ala Ser Ala Arg Leu Ala Leu Pro Glu Pro Glu Glu Ser Asp Arg
        995                 1000                1005            
Thr Ser Val Met Ile His Glu Arg Arg Ser Lys Arg Lys Pro Val Val
    1010                1015                1020                
Val Asn Phe Arg Pro Ser Leu Phe Leu Tyr Gln Glu Lys Glu Gln Glu
1025                1030                1035                1040
Ala Gln Arg Ser Gln Asn Cys Glu Pro Ile Ile Glu Glu Pro Ala Ser
                1045                1050                1055    
Pro Glu Pro Glu Tyr Ile Glu His Asp Ile Glu Asp Tyr Pro Arg Asp
            1060                1065                1070        
Lys Asn Asn Val Gly Thr Ser Glu Asp Pro Trp Glu Asn Lys Asp Val
        1075                1080                1085            
Ile Pro Thr Ile Ile Leu Asn Lys Glu Ala Gly Thr Ser His Asp Leu
    1090                1095                1100                
Val Val Asn Lys Glu Ala Gly Thr Ser His Asp Leu Val Val Leu Ser
1105                1110                1115                1120
Thr Tyr Ala Ala Ala Ile Pro Arg Arg Lys Leu Lys Ile Lys Glu Lys
                1125                1130                1135    
Leu Arg Thr Glu His His Val Phe Glu Leu Pro Asp His His Ser Ile
            1140                1145                1150        
Leu Glu Gly Phe Glu Arg Arg Glu Ala Glu Asp Ile Val Pro Tyr Leu
        1155                1160                1165            
Leu Ala Ile Trp Thr Pro Gly Glu Thr Val Asn Ser Ile Gln Pro Pro
    1170                1175                1180                
Lys Gln Arg Cys Ala Leu Phe Glu Ser Asn Asn Thr Leu Cys Asn Glu
1185                1190                1195                1200
Asn Lys Cys Phe Gln Cys Asn Lys Thr Arg Glu Glu Glu Ser Gln Thr
                1205                1210                1215    
Val Arg Gly Thr Ile Leu Ile Pro Cys Arg Thr Ala Met Arg Gly Gly
            1220                1225                1230        
Phe Pro Leu Asn Gly Thr Tyr Phe Gln Thr Asn Glu Val Phe Ala Asp
        1235                1240                1245            
His Asp Ser Ser Ile Asn Pro Ile Asp Val Pro Thr Glu Leu Ile Trp
    1250                1255                1260                
Asp Leu Lys Arg Arg Val Ala Tyr Leu Gly Ser Ser Val Ser Ser Ile
1265                1270                1275                1280
Cys Lys Gly Leu Ser Val Glu Ala Ile Lys Tyr Asn Phe Gln Glu Gly
                1285                1290                1295    
Tyr Val Cys Val Arg Gly Phe Asp Arg Glu Asn Arg Lys Pro Lys Ser
            1300                1305                1310        
Leu Val Lys Arg Leu His Cys Ser His Val Ala Ile Arg Thr Lys Glu
        1315                1320                1325            
Lys Thr Glu Glu
    1330        


<210> 8
<211> 1099
<212> PRT
<213> Arabidopsis thaliana

<220> 
<223> amino acid sequence of DML3 gene

<400> 8
Met Glu Phe Ser Ile Asp Arg Asp Lys Asn Leu Leu Met Val Val Pro
 1               5                  10                  15      
Glu Thr Arg Ile Lys Thr Lys Gln Phe Glu Lys Val Tyr Val Arg Arg
            20                  25                  30          
Lys Ser Ile Lys Leu Pro Gln Asn Ser Met Val His Asn Thr Leu Ile
        35                  40                  45              
Lys Met Ala Arg Gln Arg Ile Gln Lys Ser Met Lys Glu Ser Val Met
    50                  55                  60                  
Asn Gln His Ile Phe Lys Asn Phe Asp Ser Tyr Leu Ser Val Ile Tyr
65                  70                  75                  80  
His Pro Cys Cys Phe Val Ile Asn Asn Ser Gln Thr Thr His Lys Lys
                85                  90                  95      
Lys Glu Lys Lys Asn Ser Lys Glu Lys His Gly Ile Lys His Ser Glu
            100                 105                 110         
Ser Glu His Leu Gln Asp Asp Ile Ser Gln Arg Val Thr Gly Lys Gly
        115                 120                 125             
Arg Arg Arg Asn Ser Lys Gly Thr Pro Lys Lys Leu Arg Phe Asn Arg
    130                 135                 140                 
Pro Arg Ile Leu Glu Asp Gly Lys Lys Pro Arg Asn Pro Ala Thr Thr
145                 150                 155                 160 
Arg Leu Arg Thr Ile Ser Asn Lys Arg Arg Lys Lys Asp Ile Asp Ser
                165                 170                 175     
Glu Asp Glu Val Ile Pro Glu Leu Ala Thr Pro Thr Lys Glu Ser Phe
            180                 185                 190         
Pro Lys Arg Arg Lys Asn Glu Lys Ile Lys Arg Ser Val Ala Arg Thr
        195                 200                 205             
Leu Asn Phe Lys Gln Glu Ile Val Leu Ser Cys Leu Glu Phe Asp Lys
    210                 215                 220                 
Ile Cys Gly Pro Ile Phe Pro Arg Gly Lys Lys Arg Thr Thr Thr Arg
225                 230                 235                 240 
Arg Arg Tyr Asp Phe Leu Cys Phe Leu Leu Pro Met Pro Val Trp Lys
                245                 250                 255     
Lys Gln Ser Arg Arg Ser Lys Arg Arg Lys Asn Met Val Arg Trp Ala
            260                 265                 270         
Arg Ile Ala Ser Ser Ser Lys Leu Leu Glu Glu Thr Leu Pro Leu Ile
        275                 280                 285             
Val Ser His Pro Thr Ile Asn Gly Gln Ala Asp Ala Ser Leu His Ile
    290                 295                 300                 
Asp Asp Thr Leu Val Arg His Val Val Ser Lys Gln Thr Lys Lys Ser
305                 310                 315                 320 
Ala Asn Asn Val Ile Glu His Leu Asn Arg Gln Ile Thr Tyr Gln Lys
                325                 330                 335     
Asp His Gly Leu Ser Ser Leu Ala Asp Val Pro Leu His Ile Glu Asp
            340                 345                 350         
Thr Leu Ile Lys Ser Ala Ser Ser Val Leu Ser Glu Arg Pro Ile Lys
        355                 360                 365             
Lys Thr Lys Asp Ile Ala Lys Leu Ile Lys Asp Met Gly Arg Leu Lys
    370                 375                 380                 
Ile Asn Lys Lys Val Thr Thr Met Ile Lys Ala Asp Lys Lys Leu Val
385                 390                 395                 400 
Thr Ala Lys Val Asn Leu Asp Pro Glu Thr Ile Lys Glu Trp Asp Val
                405                 410                 415     
Leu Met Val Asn Asp Ser Pro Ser Arg Ser Tyr Asp Asp Lys Glu Thr
            420                 425                 430         
Glu Ala Lys Trp Lys Lys Glu Arg Glu Ile Phe Gln Thr Arg Ile Asp
        435                 440                 445             
Leu Phe Ile Asn Arg Met His Arg Leu Gln Gly Asn Arg Lys Phe Lys
    450                 455                 460                 
Gln Trp Lys Gly Ser Val Val Asp Ser Val Val Gly Val Phe Leu Thr
465                 470                 475                 480 
Gln Asn Thr Thr Asp Tyr Leu Ser Ser Asn Ala Phe Met Ser Val Ala
                485                 490                 495     
Ala Lys Phe Pro Val Asp Ala Arg Glu Gly Leu Ser Tyr Tyr Ile Glu
            500                 505                 510         
Glu Pro Gln Asp Ala Lys Ser Ser Glu Cys Ile Ile Leu Ser Asp Glu
        515                 520                 525             
Ser Ile Ser Lys Val Glu Asp His Glu Asn Thr Ala Lys Arg Lys Asn
    530                 535                 540                 
Glu Lys Thr Gly Ile Ile Glu Asp Glu Ile Val Asp Trp Asn Asn Leu
545                 550                 555                 560 
Arg Arg Met Tyr Thr Lys Glu Gly Ser Arg Pro Glu Met His Met Asp
                565                 570                 575     
Ser Val Asn Trp Ser Asp Val Arg Leu Ser Gly Gln Asn Val Leu Glu
            580                 585                 590         
Thr Thr Ile Lys Lys Arg Gly Gln Phe Arg Ile Leu Ser Glu Arg Ile
        595                 600                 605             
Leu Lys Phe Leu Asn Asp Glu Val Asn Gln Asn Gly Asn Ile Asp Leu
    610                 615                 620                 
Glu Trp Leu Arg Asn Ala Pro Ser His Leu Val Lys Arg Tyr Leu Leu
625                 630                 635                 640 
Glu Ile Glu Gly Ile Gly Leu Lys Ser Ala Glu Cys Val Arg Leu Leu
                645                 650                 655     
Gly Leu Lys His His Ala Phe Pro Val Asp Thr Asn Val Gly Arg Ile
            660                 665                 670         
Ala Val Arg Leu Gly Leu Val Pro Leu Glu Pro Leu Pro Asn Gly Val
        675                 680                 685             
Gln Met His Gln Leu Phe Glu Tyr Pro Ser Met Asp Ser Ile Gln Lys
    690                 695                 700                 
Tyr Leu Trp Pro Arg Leu Cys Lys Leu Pro Gln Glu Thr Leu Tyr Glu
705                 710                 715                 720 
Leu His Tyr Gln Met Ile Thr Phe Gly Lys Val Phe Cys Thr Lys Thr
                725                 730                 735     
Ile Pro Asn Cys Asn Ala Cys Pro Met Lys Ser Glu Cys Lys Tyr Phe
            740                 745                 750         
Ala Ser Ala Tyr Val Ser Ser Lys Val Leu Leu Glu Ser Pro Glu Glu
        755                 760                 765             
Lys Met His Glu Pro Asn Thr Phe Met Asn Ala His Ser Gln Asp Val
    770                 775                 780                 
Ala Val Asp Met Thr Ser Asn Ile Asn Leu Val Glu Glu Cys Val Ser
785                 790                 795                 800 
Ser Gly Cys Ser Asp Gln Ala Ile Cys Tyr Lys Pro Leu Val Glu Phe
                805                 810                 815     
Pro Ser Ser Pro Arg Ala Glu Ile Pro Glu Ser Thr Asp Ile Glu Asp
            820                 825                 830         
Val Pro Phe Met Asn Leu Tyr Gln Ser Tyr Ala Ser Val Pro Lys Ile
        835                 840                 845             
Asp Phe Asp Leu Asp Ala Leu Lys Lys Ser Val Glu Asp Ala Leu Val
    850                 855                 860                 
Ile Ser Gly Arg Met Ser Ser Ser Asp Glu Glu Ile Ser Lys Ala Leu
865                 870                 875                 880 
Val Ile Pro Thr Pro Glu Asn Ala Cys Ile Pro Ile Lys Pro Pro Arg
                885                 890                 895     
Lys Met Lys Tyr Tyr Asn Arg Leu Arg Thr Glu His Val Val Tyr Val
            900                 905                 910         
Leu Pro Asp Asn His Glu Leu Leu His Asp Phe Glu Arg Arg Lys Leu
        915                 920                 925             
Asp Asp Pro Ser Pro Tyr Leu Leu Ala Ile Trp Gln Pro Gly Glu Thr
    930                 935                 940                 
Ser Ser Ser Phe Val Pro Pro Lys Lys Lys Cys Ser Ser Asp Gly Ser
945                 950                 955                 960 
Lys Leu Cys Lys Ile Lys Asn Cys Ser Tyr Cys Trp Thr Ile Arg Glu
                965                 970                 975     
Gln Asn Ser Asn Ile Phe Arg Gly Thr Ile Leu Ile Pro Cys Arg Thr
            980                 985                 990         
Ala Met Arg Gly Ala Phe Pro Leu Asn Gly Thr Tyr Phe Gln Thr Asn
        995                 1000                1005            
Glu Val Phe Ala Asp His Glu Thr Ser Leu Asn Pro Ile Val Phe Arg
    1010                1015                1020                
Arg Glu Leu Cys Lys Gly Leu Glu Lys Arg Ala Leu Tyr Cys Gly Ser
1025                1030                1035                1040
Thr Val Thr Ser Ile Phe Lys Leu Leu Asp Thr Arg Arg Ile Glu Leu
                1045                1050                1055    
Cys Phe Trp Thr Gly Phe Leu Cys Leu Arg Ala Phe Asp Arg Lys Gln
            1060                1065                1070        
Arg Asp Pro Lys Glu Leu Val Arg Arg Leu His Thr Pro Pro Asp Glu
        1075                1080                1085            
Arg Gly Pro Lys Phe Met Ser Asp Asp Asp Ile
    1090                1095                


<210> 9
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer JH151

<400> 9
tgaagagcca ttaccacttg atcctgcagg agggaatcga gcagctag              48

<210> 10
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer JH152

<400> 10
agtggtaatg gctcttcagg aaacggtacc agccagtggg atagtctc              48

<210> 11
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer JH-RNseqF

<400> 11
cagatgtccg ctttctggta tg                                          22

<210> 12
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer JH153

<400> 12
acagtgttcg ttgatcgag                                              19

<210> 13
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer JH154

<400> 13
ttaaggatcc tacaaaggag atggtgcac                                   29

<210> 14
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer JH155

<400> 14
ttaagtcgac ttaggttttg ttgttcttca atttgc                           36
