                         SEQUENCE LISTING

<110>  Ecole de Biologie Industrielle
 
<120>  Procd de production d'une protine d'intrt grce  une 
       nouvelle protine de fusion

<130>  BCT100127

<160>  29    

<170>  PatentIn version 3.3

<210>  1
<211>  486
<212>  DNA
<213>  Spirulina platensis

<400>  1
atgagtatcg ttaccaaatc catcgtcaat gcagacgctg aagcgcgtta tctgagccct     60

ggtgaattag atcggatcaa atcctttgtt acctctggcg aacgccgggt tcggattgct    120

gaaaccatga caggtgctcg tgagcgcatc atcaaggaag caggaaacca acttttccaa    180

aaacgtcctg atgtagtctc tcccggtgga aatgcctacg gtgaggaaat gactgccacc    240

tgcctgcggg atctagacta ctacctgcgt ctgatcacct acggaattgt tgctggcgat    300

gttaccccca ttgaagaaat cggggttgta ggtgttcgcg aaatgtacaa atctcttggt    360

actcccatcg aagcagtagc tgaaggtgta cgggctatga agagtgtagc cacttccctg    420

ctgtctggag aagacgcggc cgaagcaggt gcttacttcg actacctaat tggtgcaatg    480

tcataa                                                               486


<210>  2
<211>  161
<212>  PRT
<213>  Spirulina platensis

<400>  2

Met Ser Ile Val Thr Lys Ser Ile Val Asn Ala Asp Ala Glu Ala Arg 
1               5                   10                  15      


Tyr Leu Ser Pro Gly Glu Leu Asp Arg Ile Lys Ser Phe Val Thr Ser 
            20                  25                  30          


Gly Glu Arg Arg Val Arg Ile Ala Glu Thr Met Thr Gly Ala Arg Glu 
        35                  40                  45              


Arg Ile Ile Lys Glu Ala Gly Asn Gln Leu Phe Gln Lys Arg Pro Asp 
    50                  55                  60                  


Val Val Ser Pro Gly Gly Asn Ala Tyr Gly Glu Glu Met Thr Ala Thr 
65                  70                  75                  80  


Cys Leu Arg Asp Leu Asp Tyr Tyr Leu Arg Leu Ile Thr Tyr Gly Ile 
                85                  90                  95      


Val Ala Gly Asp Val Thr Pro Ile Glu Glu Ile Gly Val Val Gly Val 
            100                 105                 110         


Arg Glu Met Tyr Lys Ser Leu Gly Thr Pro Ile Glu Ala Val Ala Glu 
        115                 120                 125             


Gly Val Arg Ala Met Lys Ser Val Ala Thr Ser Leu Leu Ser Gly Glu 
    130                 135                 140                 


Asp Ala Ala Glu Ala Gly Ala Tyr Phe Asp Tyr Leu Ile Gly Ala Met 
145                 150                 155                 160 


Ser 
    


<210>  3
<211>  303
<212>  DNA
<213>  Spirulina platensis

<400>  3
acctctggcg aacgccgggt tcggattgct gaaaccatga caggtgctcg tgagcgcatc     60

atcaaggaag caggaaacca acttttccaa aaacgtcctg atgtagtctc tcccggtgga    120

aatgcctacg gtgaggaaat gactgccacc tgcctgcggg atctagacta ctacctgcgt    180

ctgatcacct acggaattgt tgctggcgat gttaccccca ttgaagaaat cggggttgta    240

ggtgttcgcg aaatgtacaa atctcttggt actcccatcg aagcagtagc tgaaggtgta    300

cgg                                                                  303


<210>  4
<211>  101
<212>  PRT
<213>  Spirulina platensis

<400>  4

Thr Ser Gly Glu Arg Arg Val Arg Ile Ala Glu Thr Met Thr Gly Ala 
1               5                   10                  15      


Arg Glu Arg Ile Ile Lys Glu Ala Gly Asn Gln Leu Phe Gln Lys Arg 
            20                  25                  30          


Pro Asp Val Val Ser Pro Gly Gly Asn Ala Tyr Gly Glu Glu Met Thr 
        35                  40                  45              


Ala Thr Cys Leu Arg Asp Leu Asp Tyr Tyr Leu Arg Leu Ile Thr Tyr 
    50                  55                  60                  


Gly Ile Val Ala Gly Asp Val Thr Pro Ile Glu Glu Ile Gly Val Val 
65                  70                  75                  80  


Gly Val Arg Glu Met Tyr Lys Ser Leu Gly Thr Pro Ile Glu Ala Val 
                85                  90                  95      


Ala Glu Gly Val Arg 
            100     


<210>  5
<211>  183
<212>  DNA
<213>  Spirulina platensis

<400>  5
gctgaaacca tgacaggtgc tcgtgagcgc atcatcaagg aagcaggaaa ccaacttttc     60

caaaaacgtc ctgatgtagt ctctcccggt ggaaatgcct acggtgagga aatgactgcc    120

acctgcctgc gggatctaga ctactacctg cgtctgatca cctacggaat tgttgctggc    180

gat                                                                  183


<210>  6
<211>  61
<212>  PRT
<213>  Spirulina platensis

<400>  6

Ala Glu Thr Met Thr Gly Ala Arg Glu Arg Ile Ile Lys Glu Ala Gly 
1               5                   10                  15      


Asn Gln Leu Phe Gln Lys Arg Pro Asp Val Val Ser Pro Gly Gly Asn 
            20                  25                  30          


Ala Tyr Gly Glu Glu Met Thr Ala Thr Cys Leu Arg Asp Leu Asp Tyr 
        35                  40                  45              


Tyr Leu Arg Leu Ile Thr Tyr Gly Ile Val Ala Gly Asp 
    50                  55                  60      


<210>  7
<211>  396
<212>  DNA
<213>  Spirulina platensis

<400>  7
gctgaaacca tgacaggtgc tcgtgagcgc atcatcaagg aagcaggaaa ccaacttttc     60

caaaaacgtc ctgatgtagt ctctcccggt ggaaatgcct acggtgagga aatgactgcc    120

acctgcctgc gggatctaga ctactacctg cgtctgatca cctacggaat tgttgctggc    180

gatgttaccc ccattgaaga aatcggggtt gtagctgaaa ccatgacagg tgctcgtgag    240

cgcatcatca aggaagcagg aaaccaactt ttccaaaaac gtcctgatgt agtctctccc    300

ggtggaaatg cctacggtga ggaaatgact gccacctgcc tgcgggatct agactactac    360

ctgcgtctga tcacctacgg aattgttgct ggcgat                              396


<210>  8
<211>  132
<212>  PRT
<213>  Spirulina platensis

<400>  8

Ala Glu Thr Met Thr Gly Ala Arg Glu Arg Ile Ile Lys Glu Ala Gly 
1               5                   10                  15      


Asn Gln Leu Phe Gln Lys Arg Pro Asp Val Val Ser Pro Gly Gly Asn 
            20                  25                  30          


Ala Tyr Gly Glu Glu Met Thr Ala Thr Cys Leu Arg Asp Leu Asp Tyr 
        35                  40                  45              


Tyr Leu Arg Leu Ile Thr Tyr Gly Ile Val Ala Gly Asp Val Thr Pro 
    50                  55                  60                  


Ile Glu Glu Ile Gly Val Val Ala Glu Thr Met Thr Gly Ala Arg Glu 
65                  70                  75                  80  


Arg Ile Ile Lys Glu Ala Gly Asn Gln Leu Phe Gln Lys Arg Pro Asp 
                85                  90                  95      


Val Val Ser Pro Gly Gly Asn Ala Tyr Gly Glu Glu Met Thr Ala Thr 
            100                 105                 110         


Cys Leu Arg Asp Leu Asp Tyr Tyr Leu Arg Leu Ile Thr Tyr Gly Ile 
        115                 120                 125             


Val Ala Gly Asp 
    130         


<210>  9
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  9
catggactcg tactaccatc accatcacca tcaccatcac catcactgcg gccgc          55


<210>  10
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  10
gcggccgcag tgatggtgat ggtgatggtg atggtgatgg tagtacgagg c              51


<210>  11
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  11
gcggccgcgg ctcagggatg gacgagaa                                        28


<210>  12
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  12
ttaattaagg taccggatcc tggttcac                                        28


<210>  13
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  13
atcgccgagc actcagacaa g                                               21


<210>  14
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  14
aagggtttcc gaaggctt                                                   18


<210>  15
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  15
atcagcagct ttgtggaaaa gatg                                            24


<210>  16
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  16
gctggaactc ctcgccagt                                                  19


<210>  17
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  17
atggtacctg attgtcggag gaagtga                                         27


<210>  18
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  18
atgtcgactc aatgtagaaa actttgtatc ca                                   32


<210>  19
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  19
atccatggca attgtcggag gaagtgact                                       29


<210>  20
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  20
atgtcgactc acttttcgaa ctgcgggtgg ctccaatgta gaaaactttg tatcc          55


<210>  21
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  21
atccatggct agctggagcc acccgcagtt cgaaaaaggc gccggagaaa gcttgtttaa     60

g                                                                     61


<210>  22
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  22
agtacaccat cattcatgag                                                 20


<210>  23
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  23
cacgtggtgc cgcgcggcag ccatat                                          26


<210>  24
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  24
cctaggctag gattttttaa tgtta                                           25


<210>  25
<211>  769
<212>  DNA
<213>  Artificial

<220>
<223>  10xHis-SBP-Cyantag-SalI-site tev-SnabI-site 
       EK-PmlI-NdeI-SwaI-AvrII-xhoI

<400>  25
ccatggcctc gtactaccat caccatcacc atcaccatca ccatcactgc ggccgcggct     60

cagggatgga cgagaagacc actggttggc gaggtggaca cgttgttgaa ggactggctg    120

gggaacttga acaacttcgt gcacgactgg agcatcaccc acaaggtcaa cgtgaaccag    180

gatccggtac cgatatcagt atcgttacca aatccatcgt caatgcagac gctgaagcgc    240

gttatctgag ccctggtgaa ttagatcgga tcaaatcctt tgttacctct ggcgaacgcc    300

gggttcggat tgctgaaacc atgacaggtg ctcgtgagcg catcatcaag gaagcaggaa    360

accaactttt ccaaaaacgt cctgatgtag tctctcccgg tggaaatgcc tacggtgagg    420

aaatgactgc cacctgcctg cgggatctag actactacct gcgtctgatc acctacggaa    480

ttgttgctgg cgatgttacc cccattgaag aaatcggggt tgtaggtgtt cgcgaaatgt    540

acaaatctct tggtactccc atcgaagcag tagctgaagg tgtacgggct atgaagagtg    600

tagccacttc cctgctgtct ggagaagacg cggccgaagc aggtgcttac ttcgactacc    660

taattggtgc aatgtcagag ctcgggcacg tcgacgaaaa cctgtatttt cagggctacg    720

tagacgacga cgacaagcac gtgcatatga tttaaatcct aggctcgag                769


<210>  26
<211>  876
<212>  DNA
<213>  Artificial

<220>
<223>  enzyme

<400>  26
atgcaggatt ctgaatcaaa aaccccaggt gcatcccccc tagtgggcgc agataatgcg     60

cctttaactg ggggagaagc tgaagggggt gatgcttgga cagtagaaca ggcgatcgcc    120

gccctaaacc atcaagaacc aaatcgtcgc tattatgcgg cttggtggtt ggggcgtttt    180

cgtgtgtgtg atgcgatcgc agttgatgcc ttaattatgg ccttggatga tgagtccgat    240

cgcactaagg acgggggcta tcctttacgc agaaatgcgg ctcgggcttt gggtaaattg    300

ggcgatcgtc gcgcggtgac tcccctagtc aaatgtttag attgccaaga tttttatgtg    360

cgggaagcgg cggctcaggc tttagaaggt ctgggagact ctagctgtgt ttctgctttg    420

cgtgggttat tggtgggtgg agtagaagat actgcaccga tagcgggtaa accccattta    480

aaacagcctt atgacgcagt aattgaagcc ataggaactc tgggcgcaac tgaggcgtta    540

actgatattg aacctttcat aaaccatccg atcgctagaa tacagtatgc ggctttaagg    600

gctatgtatc agcttacggg tgatccggtt tatggcgatc gcttaattgc agctttgcgg    660

ggggaagatt tacaattgcg acgcagtgct ttaatggatt taggggcgat cggatatctt    720

caggccgccg aacctatcgc caacacccta gctgaaaata gcctgaaact aattgcttta    780

cagggaattc tggaacacca tctcagtcaa attggggaaa aatgcctatc tgaagaggca    840

gttaaaatca tgaagctgat ggattctcta ttatga                              876


<210>  27
<211>  615
<212>  DNA
<213>  Artificial

<220>
<223>  enzyme

<400>  27
atgacaaagg ttgaggaact aattttagcg gttgaacagg cggactctgc tggaaaactg     60

ataggcgcgg tgtcacaatt ggccgcgact ggttctgtgg atgcagtacc aatgttaatt    120

cgtgttttgg gttataacaa ccctggcgcg gcggtggctg cagttgaggg attgatagcc    180

attggtaaac ctgctgtggt tggtatacta gagaggttag atggttataa ctatggtgct    240

agggcttggg cggttcgcgc tttgtctggt attggtgatc ctaggggttt agatgttttg    300

ctggaggcgg cgggtagtga ttttgctctg agtgtgcgtc gtgctgcggc gcggggtttg    360

ggagcaattc gctgggagga tatgtcacca gaagcagtct ctgaggctaa ggaaagaacc    420

ctaaaaactc tgctgcatac ttctgaagat acggaatgga tcgttcgtta tgcaacggtt    480

gtgggtttgg aaggtttggc ggcttctgag gtggataaac cccattggtt tacacaggtt    540

tcgcaaggtt ggaggggatg gttaataatg atgaaactcc ggcggtttct gctagggcta    600

gtctggctag gatga                                                     615


<210>  28
<211>  723
<212>  DNA
<213>  Artificial

<220>
<223>  enzyme

<400>  28
atgagtgtca acttagcttc ccagttgcgg gaagggacga aaaaatccca ctccatggcg     60

gagaacgtcg gctttgtcaa atgcttcctc aagggcgttg tcgagaaaaa ttcctaccgt    120

aagctggttg gcaatctcta ctttgtctac agtgccatgg aagaggaaat ggcaaaattt    180

aaggaccatc ccatcctcag ccacatttac ttccccgaac tcaaccgcaa acaaagccta    240

gagcaagacc tgcaattcta ttacggctcc aactggcggc aagaagtgaa aatttctgcc    300

gctggccaag cctatgtgga ccgagtccgg caagtggccg ctacggcccc tgaattgttg    360

gtggcccatt cctacacccg ttacctgggg gatctttccg gcggtcaaat tctcaagaaa    420

attgcccaaa atgccatgaa tctccacgat ggtggcacag ctttctatga atttgccgac    480

attgatgacg aaaaggcttt taaaaatacc taccgtcaag ctatgaatga tctgcccatt    540

gaccaagcca ccgccgaacg gattgtggat gaagccaatg acgcctttgc catgaacatg    600

aaaatgttca acgaacttga aggcaacctg atcaaggcga tcggcattat ggtgttcaac    660

agcctcaccc gtcgccgcag tcaaggcagc accgaagttg gcctcgccac ctccgaaggc    720

tag                                                                  723


<210>  29
<211>  747
<212>  DNA
<213>  Artificial

<220>
<223>  enzyme

<400>  29
atggccgtca ctgatttaag tttgaccaat tcttccctga tgcctacgtt gaacccgatg     60

attcaacagt tggccctggc gatcgccgct agttggcaaa gtttacccct caagccctat    120

caattgccgg aggatttggg ctacgtagaa ggccgcctgg aaggggaaaa gttagtgatt    180

gaaaatcggt gctaccaaac gccccagttt cgcaaaatgc atttggagtt ggccaaggtg    240

ggcaaagggt tggatattct ccactgtgta atgtttcctg agcctttata cggtctacct    300

ttgtttggct gtgacattgt ggccggcccc ggtggagtaa gtgcggctat tgcggatcta    360

tcccccaccc aaagcgatcg ccaattgccc gcagcgtacc aaaaatcatt ggcagagcta    420

ggccagccag aatttgagca acaacgggaa ttgcccccct ggggagaaat attttctgaa    480

tattgtttat tcatccgtcc cagcaatgtc actgaagaag aaagatttgt acaaagggta    540

gtggactttt tgcaaattca ttgtcaccaa tccatcgttg ccgaaccctt gtctgaagct    600

caaactttgg agcaccgtca ggggcaaatt cattactgcc aacaacaaca gaaaaatgat    660

aaaacccgtc gggtactgga aaaagctttt ggggaagctt gggcggaacg gtatatgagc    720

caagtcttat ttgatgttat ccaataa                                        747


