

SEQUENCE LISTING

<110> TRELLIS  BIOSCIENCE, INC.
      KEYT, Bruce
      KAUVAR, Lawrence M.
      COLLARINI, Ellen J.
      FOORD, Orit
      SPERINDE, Gizette
      FATHOLAHI, Marjan
      NGUYEN, Hung

<120> IMPROVED ANTIBODIES IMMUNOREACTIVE WITH HEREGULIN-COUPLED HER3

<130> 388512012240

<140> PCT/US2010/033058   
<141> 2010-04-29  

<150> US 61/173,670       
<151> 2009-04-29  

<160> 12

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 354
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically constructed 1G4 heavy chain VHJ558,
      DFL16.2, JH1

<220> 
<221> CDS            
<222> (3)...(354)

<400> 1
ct ggg gct tca gtg aag atg tcc tgc aag gct tct gga tac aca ttc    47
   Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe
    1               5                   10                  15
 
act gac tat gtt ata agc tgg gtg aaa cag aga act gga cag ggc ctt   95
Thr Asp Tyr Val Ile Ser Trp Val Lys Gln Arg Thr Gly Gln Gly Leu
                 20                  25                  30
 
gag tgg att gga gag att tat cct gga agt ggt aga tat tac cac aat   143
Glu Trp Ile Gly Glu Ile Tyr Pro Gly Ser Gly Arg Tyr Tyr His Asn
             35                  40                  45
 
gag aag ttc aag ggc aag gcc aca ctg act gca gac aaa tcc tcc aac   191
Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Asn
         50                  55                  60
 
aca gcc tac ata caa ctc agc agc ctg aca tct gag gac tct gcg gtc   239
Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
     65                  70                  75
 
tat ttc tgt aca agg tca tta caa cgg cta cgg tac ttc gat gtc tgg   287
Tyr Phe Cys Thr Arg Ser Leu Gln Arg Leu Arg Tyr Phe Asp Val Trp
 80                  85                  90                  95
 
ggc gca ggg acc acg gtc acc gtc tcc tca gcc aaa aca aca ccc cca   335
Gly Ala Gly Thr Thr Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro
                100                 105                 110
 
tct gtc tat caa ttg tcc a                                         354
Ser Val Tyr Gln Leu Ser
            115
 

<210> 2
<211> 117
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetically constructed 1G4 heavy chain VHJ558,
      DFL16.2, JH1 deduced amino acid sequence

<400> 2
Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr
 1               5                  10                  15      
Asp Tyr Val Ile Ser Trp Val Lys Gln Arg Thr Gly Gln Gly Leu Glu
            20                  25                  30          
Trp Ile Gly Glu Ile Tyr Pro Gly Ser Gly Arg Tyr Tyr His Asn Glu
        35                  40                  45              
Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr
    50                  55                  60                  
Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr
65                  70                  75                  80  
Phe Cys Thr Arg Ser Leu Gln Arg Leu Arg Tyr Phe Asp Val Trp Gly
                85                  90                  95      
Ala Gly Thr Thr Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser
            100                 105                 110         
Val Tyr Gln Leu Ser
        115         


<210> 3
<211> 354
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically constructed complement of 1G4 heavy
      chain VHJ558, DFL16.2, JH1

<400> 3
gaccccgaag tcacttctac aggacgttcc gaagacctat gtgtaagtga ctgatacaat 60
attcgaccca ctttgtctct tgacctgtcc cggaactcac ctaacctctc taaataggac 120
cttcaccatc tataatggtg ttactcttca agttcccgtt ccggtgtgac tgacgtctgt 180
ttaggaggtt gtgtcggatg tatgttgagt cgtcggactg tagactcctg agacgccaga 240
taaagacatg ttccagtaat gttgccgatg ccatgaagct acagaccccg cgtccctggt 300
gccagtggca gaggagtcgg ttttgttgtg ggggtagaca gatagttaac aggt       354

<210> 4
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic construct

<220>
<221> PEPTIDE        
<222> (1)...(9)
<223> CDRH1 of 1G4 heavy chain VHJ558, DFL16.2, JH1

<400> 4
Gly Tyr Thr Phe Thr Asp Tyr Val Ser
 1               5                  


<210> 5
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic construct

<220>
<221> PEPTIDE        
<222> (1)...(7)
<223> CDRH2 of 1G4 heavy chain VHJ558, DFL16.2, JH1

<400> 5
Ile Tyr Pro Ser Gly Arg Tyr
 1               5          


<210> 6
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic construct

<220>
<221> PEPTIDE        
<222> (1)...(12)
<223> CDRH3 of 1G4 heavy chain VHJ558, DFL16.2, JH1

<400> 6
Thr Arg Ser Leu Gln Arg Leu Arg Tyr Phe Asp Val
 1               5                  10          


<210> 7
<211> 360
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically constructed 1G4 light chain
      IGKV5-45, JK5 kappa

<220>
<221> CDS            
<222> (1)...(360)

<400> 7
cag tct cca gcc acc ctg tct gtg act cca gga gat aga gtc agt ctt   48
Gln Ser Pro Ala Thr Leu Ser Val Thr Pro Gly Asp Arg Val Ser Leu
 1               5                   10                  15
 
tcc tgc agg gcc agt caa agt att agt gac tac ctt cac tgg tat caa   96
Ser Cys Arg Ala Ser Gln Ser Ile Ser Asp Tyr Leu His Trp Tyr Gln
             20                  25                  30
 
cag aaa tca cat gag tct cca agg cta ctc atc aag tat ggt tcc cag   144
Gln Lys Ser His Glu Ser Pro Arg Leu Leu Ile Lys Tyr Gly Ser Gln
         35                  40                  45
 
tcc atc tct ggg atc ccc tcc agg ttc agt ggc agt gga tca ggg aca   192
Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr
     50                  55                  60
 
gat ttc act ctc agt atc agc agt gtg gag act gaa gat ttt gga atg   240
Asp Phe Thr Leu Ser Ile Ser Ser Val Glu Thr Glu Asp Phe Gly Met
 65                  70                  75                  80
 
tat ttc tgt caa cag agt aac agc tgg cct ctc acg ttc ggt gct ggg   288
Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro Leu Thr Phe Gly Ala Gly
                 85                  90                  95
 
acc aag ctg gag ctg aaa cgg gct gat gct gca cca act gta tcc gca   336
Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ala
            100                 105                 110
 
tgc acc cga ctt tgc ccg act acg                                   360
Cys Thr Arg Leu Cys Pro Thr Thr
        115                 120
 

<210> 8
<211> 120
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetically constructed 1G4 light chain
      IGKV5-45, JK5 kappa deduced amino acid sequence

<400> 8
Gln Ser Pro Ala Thr Leu Ser Val Thr Pro Gly Asp Arg Val Ser Leu
 1               5                  10                  15      
Ser Cys Arg Ala Ser Gln Ser Ile Ser Asp Tyr Leu His Trp Tyr Gln
            20                  25                  30          
Gln Lys Ser His Glu Ser Pro Arg Leu Leu Ile Lys Tyr Gly Ser Gln
        35                  40                  45              
Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr
    50                  55                  60                  
Asp Phe Thr Leu Ser Ile Ser Ser Val Glu Thr Glu Asp Phe Gly Met
65                  70                  75                  80  
Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro Leu Thr Phe Gly Ala Gly
                85                  90                  95      
Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ala
            100                 105                 110         
Cys Thr Arg Leu Cys Pro Thr Thr
        115                 120 


<210> 9
<211> 360
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically constructed complement of 1G4 light
      chain IGKV5-45, JK5 kappa

<400> 9
gtcagaggtc ggtgggacag acactgaggt cctctatctc agtcagaaag gacgtcccgg 60
tcagtttcat aatcactgat ggaagtgacc atagttgtct ttagtgtact cagaggttcc 120
gatgagtagt tcataccaag ggtcaggtag agaccctagg ggaggtccaa gtcaccgtca 180
cctagtccct gtctaaagtg agagtcatag tcgtcacacc tctgacttct aaaaccttac 240
ataaagacag ttgtctcatt gtcgaccgga gagtgcaagc cacgaccctg gttcgacctc 300
gactttgccc gactacgacg tggttgacat aggcgtacgt gggctgaaac gggctgatgc 360


<210> 10
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic construct

<220>
<221> PEPTIDE        
<222> (1)...(11)
<223> CDRL1 of 1G4 light chain IGKV5-45, JK5 kappa

<400> 10
Arg Ala Ser Gln Ser Ile Ser Asp Tyr Leu His
 1               5                  10      


<210> 11
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic construct

<220>
<221> PEPTIDE        
<222> (1)...(7)
<223> CDRL2 of 1G4 light chain IGKV5-45, JK5 kappa

<400> 11
Tyr Gly Ser Gln Ser Ile Ser
 1               5          


<210> 12
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic construct

<220>
<221> PEPTIDE        
<222> (1)...(9)
<223> CDRL3 of 1G4 light chain IGKV5-45, JK5 kappa

<400> 12
Gln Gln Ser Asn Ser Trp Pro Leu Thr
 1               5                  

