                         SEQUENCE LISTING

<110>  Robbins, Thomas Charles
       Palais, Robert Andrew
       Wittwer, Carl Thomas
 
<120>  Systems and Methods for Automated Melting Curve Analysis

<130>  35328/101

<150>  US 61/178,886
<151>  2009-05-15

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized sequence for hairpin melting curve studies


<220>
<221>  stem_loop
<222>  (8)..(12)

<220>
<221>  stem_loop
<222>  (23)..(27)

<400>  1
gttaaccact gatagcacga cgtcagt                                           27


<210>  2
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized sequence for hairpin melting curve studies


<220>
<221>  stem_loop
<222>  (8)..(14)

<220>
<221>  stem_loop
<222>  (25)..(31)

<400>  2
gttaaccact gacatagcac gacgtgtcag t                                      31


<210>  3
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized sequence for hairpin melting curve studies


<220>
<221>  stem_loop
<222>  (8)..(16)

<220>
<221>  stem_loop
<222>  (27)..(35)

<400>  3
gttaaccact gacagttagc acgacgactg tcagt                                  35


<210>  4
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized sequence for melting curve studies

<400>  4
atcgtgattt ctatagttat ctaagtagtt ggcattaata atttcatttt                  50


<210>  5
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized sequence for melting curve studies


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  locked nucleic acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  locked nucleic acid

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  locked nucleic acid

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  locked nucleic acid

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  locked nucleic acid

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  locked nucleic acid

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  locked nucleic acid

<220>
<221>  misc_feature
<222>  (50)..(50)
<223>  locked nucleic acid

<400>  5
gcggtcagtc ggcctagcgg tagccagctg cggcactgcg tgacgctcag                  50


<210>  6
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  6
atggcaagct tggaattagc                                                   20


<210>  7
<211>  40
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Intentional 2-base mismatch

<220>
<221>  misc_feature
<222>  (3)..(21)
<223>  probe element

<220>
<221>  primer_bind
<222>  (3)..(21)

<220>
<221>  variation
<222>  (12)..(12)

<400>  7
ggtctgcaga ccgaatgtat gcctaagcca gcgtgttaga                             40


