                         SEQUENCE LISTING

<110>  BASF SE
       
 
<120>  A process  for the enzymatic reduction of enoates

<130>  PF62172

<160>  4     

<170>  PatentIn version 3.4

<210>  1
<211>  399
<212>  PRT
<213>  saccharomyces carlsbergensis

<400>  1

Ser Phe Val Lys Asp Phe Lys Pro Gln Ala Leu Gly Asp Thr Asn Leu 
1               5                   10                  15      


Phe Lys Pro Ile Lys Ile Gly Asn Asn Glu Leu Leu His Arg Ala Val 
            20                  25                  30          


Ile Pro Pro Leu Thr Arg Met Arg Ala Leu His Pro Gly Asn Ile Pro 
        35                  40                  45              


Asn Arg Asp Trp Ala Val Glu Tyr Tyr Thr Gln Arg Ala Gln Arg Pro 
    50                  55                  60                  


Gly Thr Met Ile Ile Thr Glu Gly Ala Phe Ile Ser Pro Gln Ala Gly 
65                  70                  75                  80  


Gly Tyr Asp Asn Ala Pro Gly Val Trp Ser Glu Glu Gln Met Val Glu 
                85                  90                  95      


Trp Thr Lys Ile Phe Asn Ala Ile His Glu Lys Lys Ser Phe Val Trp 
            100                 105                 110         


Val Gln Leu Trp Val Leu Gly Trp Ala Ala Phe Pro Asp Asn Leu Ala 
        115                 120                 125             


Arg Asp Gly Leu Arg Tyr Asp Ser Ala Ser Asp Asn Val Phe Met Asp 
    130                 135                 140                 


Ala Glu Gln Glu Ala Lys Ala Lys Lys Ala Asn Asn Pro Gln His Ser 
145                 150                 155                 160 


Leu Thr Lys Asp Glu Ile Lys Gln Tyr Ile Lys Glu Tyr Val Gln Ala 
                165                 170                 175     


Ala Lys Asn Ser Ile Ala Ala Gly Ala Asp Gly Val Glu Ile His Ser 
            180                 185                 190         


Ala Asn Gly Tyr Leu Leu Asn Gln Phe Leu Asp Pro His Ser Asn Thr 
        195                 200                 205             


Arg Thr Asp Glu Tyr Gly Gly Ser Ile Glu Asn Arg Ala Arg Phe Thr 
    210                 215                 220                 


Leu Glu Val Val Asp Ala Leu Val Glu Ala Ile Gly His Glu Lys Val 
225                 230                 235                 240 


Gly Leu Arg Leu Ser Pro Tyr Gly Val Phe Asn Ser Met Ser Gly Gly 
                245                 250                 255     


Ala Glu Thr Gly Ile Val Ala Gln Tyr Ala Tyr Val Ala Gly Glu Leu 
            260                 265                 270         


Glu Lys Arg Ala Lys Ala Gly Lys Arg Leu Ala Phe Val His Leu Val 
        275                 280                 285             


Glu Pro Arg Val Thr Asn Pro Phe Leu Thr Glu Gly Glu Gly Glu Tyr 
    290                 295                 300                 


Glu Gly Gly Ser Asn Asp Phe Val Tyr Ser Ile Trp Lys Gly Pro Val 
305                 310                 315                 320 


Ile Arg Ala Gly Asn Phe Ala Leu His Pro Glu Val Val Arg Glu Glu 
                325                 330                 335     


Val Lys Asp Lys Arg Thr Leu Ile Gly Tyr Gly Arg Phe Phe Ile Ser 
            340                 345                 350         


Asn Pro Asp Leu Val Asp Arg Leu Glu Lys Gly Leu Pro Leu Asn Lys 
        355                 360                 365             


Tyr Asp Arg Asp Thr Phe Tyr Gln Met Ser Ala His Gly Tyr Ile Asp 
    370                 375                 380                 


Tyr Pro Thr Tyr Glu Glu Ala Leu Lys Leu Gly Trp Asp Lys Lys 
385                 390                 395                 


<210>  2
<211>  399
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Pro Phe Val Lys Asp Phe Lys Pro Gln Ala Leu Gly Asp Thr Asn Leu 
1               5                   10                  15      


Phe Lys Pro Ile Lys Ile Gly Asn Asn Glu Leu Leu His Arg Ala Val 
            20                  25                  30          


Ile Pro Pro Leu Thr Arg Met Arg Ala Gln His Pro Gly Asn Ile Pro 
        35                  40                  45              


Asn Arg Asp Trp Ala Val Glu Tyr Tyr Ala Gln Arg Ala Gln Arg Pro 
    50                  55                  60                  


Gly Thr Leu Ile Ile Thr Glu Gly Thr Phe Pro Ser Pro Gln Ser Gly 
65                  70                  75                  80  


Gly Tyr Asp Asn Ala Pro Gly Ile Trp Ser Glu Glu Gln Ile Lys Glu 
                85                  90                  95      


Trp Thr Lys Ile Phe Lys Ala Ile His Glu Asn Lys Ser Phe Ala Trp 
            100                 105                 110         


Val Gln Leu Trp Val Leu Gly Trp Ala Ala Phe Pro Asp Thr Leu Ala 
        115                 120                 125             


Arg Asp Gly Leu Arg Tyr Asp Ser Ala Ser Asp Asn Val Tyr Met Asn 
    130                 135                 140                 


Ala Glu Gln Glu Glu Lys Ala Lys Lys Ala Asn Asn Pro Gln His Ser 
145                 150                 155                 160 


Ile Thr Lys Asp Glu Ile Lys Gln Tyr Val Lys Glu Tyr Val Gln Ala 
                165                 170                 175     


Ala Lys Asn Ser Ile Ala Ala Gly Ala Asp Gly Val Glu Ile His Ser 
            180                 185                 190         


Ala Asn Gly Tyr Leu Leu Asn Gln Phe Leu Asp Pro His Ser Asn Asn 
        195                 200                 205             


Arg Thr Asp Glu Tyr Gly Gly Ser Ile Glu Asn Arg Ala Arg Phe Thr 
    210                 215                 220                 


Leu Glu Val Val Asp Ala Val Val Asp Ala Ile Gly Pro Glu Lys Val 
225                 230                 235                 240 


Gly Leu Arg Leu Ser Pro Tyr Gly Val Phe Asn Ser Met Ser Gly Gly 
                245                 250                 255     


Ala Glu Thr Gly Ile Val Ala Gln Tyr Ala Tyr Val Leu Gly Glu Leu 
            260                 265                 270         


Glu Arg Arg Ala Lys Ala Gly Lys Arg Leu Ala Phe Val His Leu Val 
        275                 280                 285             


Glu Pro Arg Val Thr Asn Pro Phe Leu Thr Glu Gly Glu Gly Glu Tyr 
    290                 295                 300                 


Asn Gly Gly Ser Asn Lys Phe Ala Tyr Ser Ile Trp Lys Gly Pro Ile 
305                 310                 315                 320 


Ile Arg Ala Gly Asn Phe Ala Leu His Pro Glu Val Val Arg Glu Glu 
                325                 330                 335     


Val Lys Asp Pro Arg Thr Leu Ile Gly Tyr Gly Arg Phe Phe Ile Ser 
            340                 345                 350         


Asn Pro Asp Leu Val Asp Arg Leu Glu Lys Gly Leu Pro Leu Asn Lys 
        355                 360                 365             


Tyr Asp Arg Asp Thr Phe Tyr Lys Met Ser Ala Glu Gly Tyr Ile Asp 
    370                 375                 380                 


Tyr Pro Thr Tyr Glu Glu Ala Leu Lys Leu Gly Trp Asp Lys Asn 
385                 390                 395                 


<210>  3
<211>  338
<212>  PRT
<213>  Bacillus subtilis

<400>  3

Met Ala Arg Lys Leu Phe Thr Pro Ile Thr Ile Lys Asp Met Thr Leu 
1               5                   10                  15      


Lys Asn Arg Ile Val Met Ser Pro Met Cys Met Tyr Ser Ser His Glu 
            20                  25                  30          


Lys Asp Gly Lys Leu Thr Pro Phe His Met Ala His Tyr Ile Ser Arg 
        35                  40                  45              


Ala Ile Gly Gln Val Gly Leu Ile Ile Val Glu Ala Ser Ala Val Asn 
    50                  55                  60                  


Pro Gln Gly Arg Ile Thr Asp Gln Asp Leu Gly Ile Trp Ser Asp Glu 
65                  70                  75                  80  


His Ile Glu Gly Phe Ala Lys Leu Thr Glu Gln Val Lys Glu Gln Gly 
                85                  90                  95      


Ser Lys Ile Gly Ile Gln Leu Ala His Ala Gly Arg Lys Ala Glu Leu 
            100                 105                 110         


Glu Gly Asp Ile Phe Ala Pro Ser Ala Ile Ala Phe Asp Glu Gln Ser 
        115                 120                 125             


Ala Thr Pro Val Glu Met Ser Ala Glu Lys Val Lys Glu Thr Val Gln 
    130                 135                 140                 


Glu Phe Lys Gln Ala Ala Ala Arg Ala Lys Glu Ala Gly Phe Asp Val 
145                 150                 155                 160 


Ile Glu Ile His Ala Ala His Gly Tyr Leu Ile His Glu Phe Leu Ser 
                165                 170                 175     


Pro Leu Ser Asn His Arg Thr Asp Glu Tyr Gly Gly Ser Pro Glu Asn 
            180                 185                 190         


Arg Tyr Arg Phe Leu Arg Glu Ile Ile Asp Glu Val Lys Gln Val Trp 
        195                 200                 205             


Asp Gly Pro Leu Phe Val Arg Val Ser Ala Ser Asp Tyr Thr Asp Lys 
    210                 215                 220                 


Gly Leu Asp Ile Ala Asp His Ile Gly Phe Ala Lys Trp Met Lys Glu 
225                 230                 235                 240 


Gln Gly Val Asp Leu Ile Asp Cys Ser Ser Gly Ala Leu Val His Ala 
                245                 250                 255     


Asp Ile Asn Val Phe Pro Gly Tyr Gln Val Ser Phe Ala Glu Lys Ile 
            260                 265                 270         


Arg Glu Gln Ala Asp Met Ala Thr Gly Ala Val Gly Met Ile Thr Asp 
        275                 280                 285             


Gly Ser Met Ala Glu Glu Ile Leu Gln Asn Gly Arg Ala Asp Leu Ile 
    290                 295                 300                 


Phe Ile Gly Arg Glu Leu Leu Arg Asp Pro Phe Phe Ala Arg Thr Ala 
305                 310                 315                 320 


Ala Lys Gln Leu Asn Thr Glu Ile Pro Ala Pro Val Gln Tyr Glu Arg 
                325                 330                 335     


Gly Trp 
        


<210>  4
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Estrogen binding protein

<400>  4

Met Thr Ile Glu Ser Thr Asn Ser Phe Val Val Pro Ser Asp Thr Lys 
1               5                   10                  15      


Leu Ile Asp Val Thr Pro Leu Gly Ser Thr Lys Leu Phe Gln Pro Ile 
            20                  25                  30          


Lys Val Gly Asn Asn Val Leu Pro Gln Arg Ile Ala Tyr Val Pro Thr 
        35                  40                  45              


Thr Arg Phe Arg Ala Ser Lys Asp His Ile Pro Ser Asp Leu Gln Leu 
    50                  55                  60                  


Asn Tyr Tyr Asn Ala Arg Ser Gln Tyr Pro Gly Thr Leu Ile Ile Thr 
65                  70                  75                  80  


Glu Ala Thr Phe Ala Ser Glu Arg Gly Gly Ile Asp Leu His Val Pro 
                85                  90                  95      


Gly Ile Tyr Asn Asp Ala Gln Ala Lys Ser Trp Lys Lys Ile Asn Glu 
            100                 105                 110         


Ala Ile His Gly Asn Gly Ser Phe Ser Ser Val Gln Leu Trp Tyr Leu 
        115                 120                 125             


Gly Arg Val Ala Asn Ala Lys Asp Leu Lys Asp Ser Gly Leu Pro Leu 
    130                 135                 140                 


Ile Ala Pro Ser Ala Val Tyr Trp Asp Glu Asn Ser Glu Lys Leu Ala 
145                 150                 155                 160 


Lys Glu Ala Gly Asn Glu Leu Arg Ala Leu Thr Glu Glu Glu Ile Asp 
                165                 170                 175     


His Ile Val Glu Val Glu Tyr Pro Asn Ala Ala Lys His Ala Leu Glu 
            180                 185                 190         


Ala Gly Phe Asp Tyr Val Glu Ile His Gly Ala His Gly Tyr Leu Leu 
        195                 200                 205             


Asp Gln Phe Leu Asn Leu Ala Ser Asn Lys Arg Thr Asp Lys Tyr Gly 
    210                 215                 220                 


Cys Gly Ser Ile Glu Asn Arg Ala Arg Leu Leu Leu Arg Val Val Asp 
225                 230                 235                 240 


Lys Leu Ile Glu Val Val Gly Ala Asn Arg Leu Ala Leu Arg Leu Ser 
                245                 250                 255     


Pro Trp Ala Ser Phe Gln Gly Met Glu Ile Glu Gly Glu Glu Ile His 
            260                 265                 270         


Ser Tyr Ile Leu Gln Gln Leu Gln Gln Arg Ala Asp Asn Gly Gln Gln 
        275                 280                 285             


Leu Ala Tyr Ile Ser Leu Val Glu Pro Arg Val Thr Gly Ile Tyr Asp 
    290                 295                 300                 


Val Ser Leu Lys Asp Gln Gln Gly Arg Ser Asn Glu Phe Ala Tyr Lys 
305                 310                 315                 320 


Ile Trp Lys Gly Asn Phe Ile Arg Ala Gly Asn Tyr Thr Tyr Asp Ala 
                325                 330                 335     


Trp Pro Glu Phe Lys Thr Leu Ile Asn Asp Leu Lys Asn Asp Arg Ser 
            340                 345                 350         


Ile Ile Gly Phe Ser Arg Phe Phe Thr Ser Asn Pro Asp Leu Val Glu 
        355                 360                 365             


Lys Leu Lys Leu Gly Lys Pro Leu Asn Tyr Tyr Asn Arg Glu Glu Phe 
    370                 375                 380                 


Tyr Lys Tyr Tyr Asn Tyr Gly Tyr Asn Ser Tyr Asp Glu Ser Glu Lys 
385                 390                 395                 400 


Gln Val Ile Gly Lys Pro Leu Ala 
                405             


