                         SEQUENCE LISTING

<110>  The Provost Fellows and Scholars of the Holy and Undivided 
       Trinity of Queen Elizabeth near Dublin
 
<120>  A Method for Improving Gene Expression

<130>  31738WO

<150>  GB 0909689.2
<151>  2009-06-05

<160>  52    

<170>  PatentIn version 3.3

<210>  1
<211>  717
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  gfp+ from pZep08


<220>
<221>  CDS
<222>  (1)..(714)

<400>  1
atg agt aaa gga gaa gaa ctt ttc act gga gtt gtc cca att ctt gtt       48
Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val         
1               5                   10                  15              

gaa tta gat ggt gat gtt aat ggg cac aaa ttt tct gtc agt gga gag       96
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu         
            20                  25                  30                  

ggt gaa ggt gat gct aca tac gga aag ctt acc ctt aaa ttt att tgc      144
Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys         
        35                  40                  45                      

act act gga aaa cta cct gtt cca tgg cca aca ctt gtc act act ttg      192
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu         
    50                  55                  60                          

acc tat ggt gtt caa tgc ttt tcc cgt tat ccg gat cat atg aaa cgg      240
Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg         
65                  70                  75                  80          

cat gac ttt ttc aag agt gcc atg ccc gaa ggt tat gta cag gaa cgc      288
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg         
                85                  90                  95              

act ata tct ttc aaa gat gac ggg aac tac aag acg cgt gct gaa gtc      336
Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val         
            100                 105                 110                 

aag ttt gaa ggt gat acc ctt gtt aat cgt atc gag tta aaa ggt att      384
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile         
        115                 120                 125                     

gat ttt aaa gaa gat gga aac att ctc gga cac aaa ctc gag tac aac      432
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn         
    130                 135                 140                         

tat aac tca cac aat gta tac atc acg gca gac aaa caa aag aat gga      480
Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly         
145                 150                 155                 160         

atc aaa gct aac ttc aaa att cgc cac aac att gaa gat gga tcc gtt      528
Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val         
                165                 170                 175             

caa cta gca gac cat tat caa caa aat act cca att ggc gat ggc cct      576
Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro         
            180                 185                 190                 

gtc ctt tta cca gac aac cat tac ctg tcg aca caa tct gcc ctt tcg      624
Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser         
        195                 200                 205                     

aaa gat ccc aac gaa aag cgt gac cac atg gtc ctt ctt gag ttt gta      672
Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val         
    210                 215                 220                         

act gct gct ggg att aca cat ggc atg gat gag ctc tac aaa taa          717
Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys                 
225                 230                 235                             


<210>  2
<211>  238
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic Construct

<400>  2

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  3
<211>  717
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  gpfT


<220>
<221>  CDS
<222>  (1)..(714)

<400>  3
atg agc aaa ggc gaa gag ctg ttc acc ggt gtt gtc ccg att ctg gtg       48
Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val         
1               5                   10                  15              

gaa ctg gac ggt gac gtt aat ggt cat aag ttt agc gtg agc ggc gag       96
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu         
            20                  25                  30                  

ggt gaa ggt gac gcc acg tac ggc aaa ctg acc ctg aaa ttc att tgc      144
Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys         
        35                  40                  45                      

acc acc ggt aag ctg ccg gtc ccg tgg cca act ttg gtg acc acc ctg      192
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu         
    50                  55                  60                          

acc tac ggt gtc cag tgt ttt agc cgt tac ccg gac cac atg aag cgt      240
Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg         
65                  70                  75                  80          

cac gac ttt ttc aag agc gca atg cct gag ggc tat gtt caa gaa cgt      288
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg         
                85                  90                  95              

act atc tcg ttt aag gat gat ggc aat tac aag acg cgt gcc gaa gtt      336
Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val         
            100                 105                 110                 

aag ttc gag ggc gac acg ctg gtt aat cgc att gag ctg aaa ggc att      384
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile         
        115                 120                 125                     

gat ttc aaa gag gat ggt aac atc ctg ggt cac aaa ctg gag tat aac      432
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn         
    130                 135                 140                         

tac aac tct cat aat gtg tat atc acg gcg gac aaa cag aag aac ggt      480
Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly         
145                 150                 155                 160         

atc aag gcg aat ttc aaa atc cgt cac aac att gaa gat ggt agc gtg      528
Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val         
                165                 170                 175             

caa ctg gca gat cat tac caa cag aat acc ccg atc ggc gac ggt ccg      576
Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro         
            180                 185                 190                 

gtt ttg ctg ccg gac aat cat tac ctg agc acg caa tcc gcg ctg agc      624
Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser         
        195                 200                 205                     

aaa gat ccg aac gaa aaa cgc gac cac atg gtc ttg ctg gaa ttt gtc      672
Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val         
    210                 215                 220                         

acc gct gcg ggc atc acc cac ggt atg gat gaa ctg tat aag taa          717
Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys                 
225                 230                 235                             


<210>  4
<211>  238
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic Construct

<400>  4

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  5
<211>  717
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Gfpmut2


<220>
<221>  CDS
<222>  (1)..(714)

<400>  5
atg agt aaa gga gaa gaa ctt ttc act gga gtt gtc cca att ctt gtt       48
Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val         
1               5                   10                  15              

gaa tta gat ggc gat gtt aat ggg caa aaa ttc tct gtc agt gga gag       96
Glu Leu Asp Gly Asp Val Asn Gly Gln Lys Phe Ser Val Ser Gly Glu         
            20                  25                  30                  

ggt gaa ggt gat gca aca tac gga aaa ctt acc ctt aaa ttt att tgc      144
Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys         
        35                  40                  45                      

act act ggg aag cta cct gtt cca tgg cca aca ctt gtc act act ttc      192
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe         
    50                  55                  60                          

gcg tat ggt ctt caa tgc ttt gcg aga tac cca gat cat atg aaa cag      240
Ala Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln         
65                  70                  75                  80          

cat gac ttt ttc aag agt gcc atg ccc gaa ggt tat gta cag gaa aga      288
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg         
                85                  90                  95              

act ata ttt tac aaa gat gac ggg aac tac aag aca cgt gct gaa gtc      336
Thr Ile Phe Tyr Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val         
            100                 105                 110                 

aag ttt gaa ggt gat acc ctt gtt aat aga atc gag tta aaa ggt att      384
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile         
        115                 120                 125                     

gat ttt aaa gaa gat gga aac att ctt gga cac aaa atg gaa tac aac      432
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Met Glu Tyr Asn         
    130                 135                 140                         

tat aac tca cat aat gta tac atc atg gca gac aaa cca aag aat gga      480
Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Pro Lys Asn Gly         
145                 150                 155                 160         

atc aaa gtt aac ttc aaa att aga cac aac att aaa gat gga agc gtt      528
Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Lys Asp Gly Ser Val         
                165                 170                 175             

caa tta gca gac cat tat caa caa aat act cca att ggc gat ggc cct      576
Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro         
            180                 185                 190                 

gtc ctt tta cca gac aac cat tac ctg tcc aca caa tct gcc ctt tcc      624
Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser         
        195                 200                 205                     

aaa gat ccc aac gaa aag aga gat cac atg atc ctt ctt gag ttt gta      672
Lys Asp Pro Asn Glu Lys Arg Asp His Met Ile Leu Leu Glu Phe Val         
    210                 215                 220                         

aca gct gct ggg att aca cat ggc atg gat gaa cta tac aaa taa          717
Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys                 
225                 230                 235                             


<210>  6
<211>  238
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic Construct

<400>  6

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly Gln Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Ala Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Tyr Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Met Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Pro Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Lys Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Ile Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  7
<211>  678
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  DsRed


<220>
<221>  CDS
<222>  (1)..(675)

<400>  7
atg agg tct tcc aag aat gtt atc aag gag ttc atg agg ttt aag gtt       48
Met Arg Ser Ser Lys Asn Val Ile Lys Glu Phe Met Arg Phe Lys Val         
1               5                   10                  15              

cgc atg gaa gga acg gtc aat ggg cac gag ttt gaa ata gaa ggc gaa       96
Arg Met Glu Gly Thr Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu         
            20                  25                  30                  

gga gag ggg agg cca tac gaa ggc cac aat acc gta aag ctt aag gta      144
Gly Glu Gly Arg Pro Tyr Glu Gly His Asn Thr Val Lys Leu Lys Val         
        35                  40                  45                      

acc aag ggg gga cct ttg cca ttt gct tgg gat att ttg tca cca caa      192
Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln         
    50                  55                  60                          

ttt cag tat gga agc aag gta tat gtc aag cac cct gcc gac ata cca      240
Phe Gln Tyr Gly Ser Lys Val Tyr Val Lys His Pro Ala Asp Ile Pro         
65                  70                  75                  80          

gac tat aaa aag ctg tca ttt cct gaa gga ttt aaa tgg gaa agg gtc      288
Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val         
                85                  90                  95              

atg aac ttt gaa gac ggt ggc gtc gtt act gta acc cag gat tcc agt      336
Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser         
            100                 105                 110                 

ttg cag gat ggc tgt ttc atc tac aag gtc aag ttc att ggc gtg aac      384
Leu Gln Asp Gly Cys Phe Ile Tyr Lys Val Lys Phe Ile Gly Val Asn         
        115                 120                 125                     

ttt cct tcc gat gga cct gtt atg caa aag aag aca atg ggc tgg gaa      432
Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu         
    130                 135                 140                         

gcc agc act gag cgt ttg tat cct cgt gat ggc gtg ttg aaa gga gag      480
Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly Glu         
145                 150                 155                 160         

att cat aag gct ctg aag ctg aaa gac ggt ggt cat tac cta gtt gaa      528
Ile His Lys Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val Glu         
                165                 170                 175             

ttc aaa agt att tac atg gca aag aag cct gtg cag cta cca ggg tac      576
Phe Lys Ser Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Tyr         
            180                 185                 190                 

tac tat gtt gac tcc aaa ctg gat ata aca agc cac aac gaa gac tat      624
Tyr Tyr Val Asp Ser Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr         
        195                 200                 205                     

aca atc gtt gag cag tat gaa aga acc gag gga cgc cac cat ctg ttc      672
Thr Ile Val Glu Gln Tyr Glu Arg Thr Glu Gly Arg His His Leu Phe         
    210                 215                 220                         

ctt taa                                                              678
Leu                                                                     
225                                                                     


<210>  8
<211>  225
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic Construct

<400>  8

Met Arg Ser Ser Lys Asn Val Ile Lys Glu Phe Met Arg Phe Lys Val 
1               5                   10                  15      


Arg Met Glu Gly Thr Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu 
            20                  25                  30          


Gly Glu Gly Arg Pro Tyr Glu Gly His Asn Thr Val Lys Leu Lys Val 
        35                  40                  45              


Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln 
    50                  55                  60                  


Phe Gln Tyr Gly Ser Lys Val Tyr Val Lys His Pro Ala Asp Ile Pro 
65                  70                  75                  80  


Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val 
                85                  90                  95      


Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser 
            100                 105                 110         


Leu Gln Asp Gly Cys Phe Ile Tyr Lys Val Lys Phe Ile Gly Val Asn 
        115                 120                 125             


Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu 
    130                 135                 140                 


Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly Glu 
145                 150                 155                 160 


Ile His Lys Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val Glu 
                165                 170                 175     


Phe Lys Ser Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Tyr 
            180                 185                 190         


Tyr Tyr Val Asp Ser Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr 
        195                 200                 205             


Thr Ile Val Glu Gln Tyr Glu Arg Thr Glu Gly Arg His His Leu Phe 
    210                 215                 220                 


Leu 
225 


<210>  9
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 1 fw Example 1

<400>  9
gggtcatacc aacgttctgg                                                 20


<210>  10
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 1 rv Example 1

<400>  10
ttgtgcccat taacatcacc                                                 20


<210>  11
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 2 fw Example 1

<400>  11
tacaagacgc gtgctgaagt                                                 20


<210>  12
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 2 rv Example 1

<400>  12
tgtgtccgag aatgtttcca                                                 20


<210>  13
<211>  21
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 3 fw Example 1

<400>  13
ggcatggatg agctctacaa a                                               21


<210>  14
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 3 rv Example 1

<400>  14
tttccttacg cgaaatacgg                                                 20


<210>  15
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 4 fw Example 1

<400>  15
tcactaccgg gcgtattttt                                                 20


<210>  16
<211>  21
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 4 rv Example 1

<400>  16
ttgagcaact gactgaaatg c                                               21


<210>  17
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 5 fw Example 1

<400>  17
tgtcggcaga atgcttaatg                                                 20


<210>  18
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  ChIP primer 5 rv Example 1

<400>  18
ctgccattca tccgcttatt                                                 20


<210>  19
<211>  60
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  fimA-gfp+-cat.fw primer of Example 3

<400>  19
gattgatgcg ggtcatacca acgttctggc tctgcagtaa tgagcgttct agatttaaga     60


<210>  20
<211>  60
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  fimA-gfp+-cat.rv primer of Example 3

<400>  20
tctgcacacc aacgtttgtt gcgctacccg cagctgaact ctacgagaca gcacattaac     60


<210>  21
<211>  60
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  kan.int.fw primer of Example 3

<400>  21
aatgtcatga taataatggt ttcttagacg tcaggtggcg tgtaggctgg agctgcttcg     60


<210>  22
<211>  59
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  kan.int.rv primer of Example 3

<400>  22
agttcagctg cgggtagcgc aacaaacgtt ggtgtgcaga catatgaata tcctcctta      59


<210>  23
<211>  60
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  +98proU-gfp+.fw primer of Example 3

<400>  23
ggcaattaaa ttagaaatta aaaatcttta taaaatataa tgagcgttct agatttaaga     60


<210>  24
<211>  60
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  +936proU-gfp+.fw primer of Example 3

<400>  24
ccgccggaca ccgaatggct taattcgtaa aaccccttaa tgagcgttct agatttaaga     60


<210>  25
<211>  57
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  +98proU-gfpT.fw primer of Example 3

<400>  25
ggcaattaaa ttagaaatta aaaatcttta taaaatatga ttgattaaga aggagat        57


<210>  26
<211>  57
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  +936proU-gfpT.fw primer of Example 3

<400>  26
ccgccggaca ccgaatggct taattcgtaa aaccccttga ttgattaaga aggagat        57


<210>  27
<211>  59
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  proU.(stop).kan.rv primer of Example 3

<400>  27
gtccgccgct ggcgtggtat cccacggatt attttgatca catatgaata tcctcctta      59


<210>  28
<211>  60
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  +98proU-lacZ.fw primer of Example 3

<400>  28
gcaattaaat tagaaattaa aaatctttat aaaatataat gagcggataa caatttcaca     60


<210>  29
<211>  60
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  +936proU-lacZ.fw primer of Example 3

<400>  29
cgccggacac cgaatggctt aattcgtaaa accccttaat gagcggataa caatttcaca     60


<210>  30
<211>  60
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  proU.(stop).cat.rv primer of Example 3

<400>  30
gtccgccgct ggcgtggtat cccacggatt attttgatca catatgaata tcctccttag     60


<210>  31
<211>  34
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  kan.Xmal.fw primer of Example 3

<400>  31
cataacgagc ccgggtgtag gctggagctg cttc                                 34


<210>  32
<211>  34
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  kan.Xmal.rv primer of Example 3

<400>  32
cataacgagc ccgggcatat gaatatcctc ctta                                 34


<210>  33
<211>  26
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  fimA.fw primer of Example 3

<400>  33
ggaaagcagc atgaaaatta aaactc                                          26


<210>  34
<211>  25
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  fimA.rv primer of Example 3

<400>  34
ggttattgat actgaacctt gaagg                                           25


<210>  35
<211>  21
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  proU.fw primer of Example 3

<400>  35
aggggttgcc ctcagattct c                                               21


<210>  36
<211>  19
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  proU.rv primer of Example 3

<400>  36
gtcagtcggt gcagtcgtc                                                  19


<210>  37
<211>  56
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  fimA-gfpT-kan fw primer of Example 4

<400>  37
gattgatgcg ggtcatacca acgttctggc tctgcaggat tcattaagaa ggagat         56


<210>  38
<211>  59
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  fimA-gfpT-kan rv primer of Example 4

<400>  38
tctgcacacc aacgtttgtt gcgctacccg cagctgaact catatgaata tcctcctta      59


<210>  39
<211>  24
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 5 gfp+.bw.fw

<400>  39
atgagtaaag gagaagaact tttc                                            24


<210>  40
<211>  22
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 5 gfp+.bs.bio.rv

<400>  40
ttatttgtag agctcatcca tg                                              22


<210>  41
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 5 gfpT.bs.fw

<400>  41
atgagcaaag gcgaagagct                                                 20


<210>  42
<211>  25
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 5 gfpT.bs.bio.rv

<400>  42
ttacttatac agttcatcca taccg                                           25


<210>  43
<211>  25
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 5 proV.bs.fw

<400>  43
agggtgttat tttcaaaaat atcac                                           25


<210>  44
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 5 proV.bs.bio.rv

<400>  44
catatgcggc attaaggcaa                                                 20


<210>  45
<211>  25
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 5 lacZ.bs.fw

<400>  45
atgaccatga ttacggattc actgg                                           25


<210>  46
<211>  20
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 5 lacZ.bs.bio.rv

<400>  46
agcgcggctg aaatcatcat                                                 20


<210>  47
<211>  678
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 6 dsredT


<220>
<221>  CDS
<222>  (1)..(675)

<400>  47
atg cgt agc tct aaa aac gtg atc aaa gaa ttc atg cgc ttc aag gta       48
Met Arg Ser Ser Lys Asn Val Ile Lys Glu Phe Met Arg Phe Lys Val         
1               5                   10                  15              

cgt atg gaa ggt acc gtg aac ggt cac gaa ttt gag att gaa ggc gag       96
Arg Met Glu Gly Thr Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu         
            20                  25                  30                  

ggc gag ggc cgc ccg tac gaa ggc cac aat acc gtt aaa ctg aaa gtt      144
Gly Glu Gly Arg Pro Tyr Glu Gly His Asn Thr Val Lys Leu Lys Val         
        35                  40                  45                      

acc aag ggt ggt ccg ctg cct ttc gca tgg gac atc ctg tct cca cag      192
Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln         
    50                  55                  60                          

ttt cag tat ggc tct aaa gta tac gtg aaa cac cca gcc gat atc ccg      240
Phe Gln Tyr Gly Ser Lys Val Tyr Val Lys His Pro Ala Asp Ile Pro         
65                  70                  75                  80          

gac tat aag aaa ctg tct ttt ccg gaa ggt ttc aaa tgg gaa cgc gta      288
Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val         
                85                  90                  95              

atg aac ttc gag gat ggc ggc gtt gtt acc gtt act caa gac tct agc      336
Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser         
            100                 105                 110                 

ctg cag gac ggc tgc ttc atc tat aag gtg aaa ttc atc ggt gtt aac      384
Leu Gln Asp Gly Cys Phe Ile Tyr Lys Val Lys Phe Ile Gly Val Asn         
        115                 120                 125                     

ttc cct agc gat ggt ccg gtg atg cag aag aag act atg ggt tgg gaa      432
Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu         
    130                 135                 140                         

gcg tct acc gaa cgt ctg tac ccg cgt gac ggt gtt ctg aaa ggc gaa      480
Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly Glu         
145                 150                 155                 160         

att cac aaa gcg ctg aaa ctg aag gac ggt ggc cac tac ctg gtt gag      528
Ile His Lys Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val Glu         
                165                 170                 175             

ttt aag agc atc tat atg gca aag aaa ccg gtc caa ctg ccg ggt tat      576
Phe Lys Ser Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Tyr         
            180                 185                 190                 

tac tac gtg gat tct aaa ctg gat att acc agc cat aac gaa gac tac      624
Tyr Tyr Val Asp Ser Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr         
        195                 200                 205                     

act atc gtt gaa cag tac gag cgc acc gaa ggc cgt cac cat ctg ttt      672
Thr Ile Val Glu Gln Tyr Glu Arg Thr Glu Gly Arg His His Leu Phe         
    210                 215                 220                         

ctg tga                                                              678
Leu                                                                     
225                                                                     


<210>  48
<211>  225
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic Construct

<400>  48

Met Arg Ser Ser Lys Asn Val Ile Lys Glu Phe Met Arg Phe Lys Val 
1               5                   10                  15      


Arg Met Glu Gly Thr Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu 
            20                  25                  30          


Gly Glu Gly Arg Pro Tyr Glu Gly His Asn Thr Val Lys Leu Lys Val 
        35                  40                  45              


Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln 
    50                  55                  60                  


Phe Gln Tyr Gly Ser Lys Val Tyr Val Lys His Pro Ala Asp Ile Pro 
65                  70                  75                  80  


Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val 
                85                  90                  95      


Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser 
            100                 105                 110         


Leu Gln Asp Gly Cys Phe Ile Tyr Lys Val Lys Phe Ile Gly Val Asn 
        115                 120                 125             


Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu 
    130                 135                 140                 


Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly Glu 
145                 150                 155                 160 


Ile His Lys Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val Glu 
                165                 170                 175     


Phe Lys Ser Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Tyr 
            180                 185                 190         


Tyr Tyr Val Asp Ser Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr 
        195                 200                 205             


Thr Ile Val Glu Gln Tyr Glu Arg Thr Glu Gly Arg His His Leu Phe 
    210                 215                 220                 


Leu 
225 


<210>  49
<211>  21
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 7 gfp+.pPro24-blunt.fw

<400>  49
agtaaaggag aagaactttt c                                               21


<210>  50
<211>  32
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 7 gfp+pPro24.Pstl.rv

<400>  50
tctactgcag ttatttgtag agctcatcca tg                                   32


<210>  51
<211>  21
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 7 gfpT.pPro24-blunt.fw

<400>  51
agcaaaggcg aagagctgtt c                                               21


<210>  52
<211>  29
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Example 7 gfpT.pPro24-Pstl.rv

<400>  52
tctactgcag ttacttatac agttcatcc                                       29


