                         SEQUENCE LISTING

<110>  The Salk Institute for Biological Studies
 
<120>  IMPROVING UNNATURAL AMINO ACID INCORPORATION IN EUKARYOTIC CELLS

<130>  7158-82954-02

<150>  US 61/184,417
<151>  2009-06-05

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify E. coli TyrRS gene

<400>  1
ccaccatgga actcgagatt ttgatggcaa gcagtaactt gattaaac                    48


<210>  2
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify E. coli TyrRS gene

<400>  2
acaagatctg ctagcttatt tccagcaaat cagacagtaa ttc                         43


<210>  3
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify the gene for EctRNA Tyr/CUA  in construct tRNA2

<400>  3
gtgggatccc cggtggggtt cccgagcggc caaagggagc agactctaaa tctgccgtca       60

tcgacttcg                                                               69


<210>  4
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify the gene for EctRNA Tyr/CUA  in construct tRNA2

<400>  4
gataagcttt tccaaaaatg gtggtggggg aaggattcga accttcgaag tcgatgacgg       60

cagatttag                                                               69


<210>  5
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify E. coli LeuRS gene

<400>  5
gcctcgagaa gagcaatacc gcccgg                                            26


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify E. coli LeuRS gene

<400>  6
cgctagctta gccaacgacc agattgagga g                                      31


<210>  7
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify EctRNA Tyr/CUA

<400>  7
aaccttcgaa gtcgatgacg gcagatttac agtctgc                                37


<210>  8
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify EctRNA Tyr/CUA

<400>  8
ccgtctaaat gtcagacgag ggaaaccggc gag                                    33


<210>  9
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify a spacer sequence from pCDNA3

<400>  9
atactagtgc gggcgctagg gcgctg                                            26


<210>  10
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify a spacer sequence from pCDNA3

<400>  10
atggtacccc tggaaggtgc cactcc                                            26


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify the E. coli TyrRS gene

<400>  11
caactagtat ggagattttg atggcaagc                                         29


<210>  12
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify the E. coli TyrRS gene

<400>  12
aactcgagtt atttccagca aatcagacag                                        30


<210>  13
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify SNR52 promoter

<400>  13
cactgcagtc tttgaaaaga taatgtatga ttatg                                  35


<210>  14
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify SNR52 promoter

<400>  14
ggccgctcgg gaaccccacc gatcatttat ctttcactgc ggag                        44


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify EctRNA Tyr/CUA gene followed by the 
       3'-flanking sequence of the SUP4 suppressor tRNA

<400>  15
ggtggggttc ccgagcggcc aaag                                              24


<210>  16
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify EctRNA Tyr/CUA gene followed by the 
       3'-flanking sequence of the SUP4 suppressor tRNA

<400>  16
ggtcgacaga cataaaaaac aaaaaaatgg tgggggaagg attcgaacct tc               52


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify RPR1 promoter

<400>  17
cactgcagtc tgccaattga acataacatg g                                      31


<210>  18
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to amplify RPR1 promoter

<400>  18
ggccgctcgg gaaccccacc tgccaatcgc agctcccaga gttt                        44


<210>  19
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify a gene cassette containing the 5' flanking
       sequence of the SUP4 suppressor tRNA, the  EctRNATyr/CUA, and the
       3' flanking sequence of the SUP4 suppressor tRNA from plasmid  
       pEYCUA-YRS-tRNA-5

<400>  19
cactgcagct ctttttcaat tgtaatgtgt tatg                                   34


<210>  20
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify a gene cassette containing the 5' flanking
       sequence of the SUP4 suppressor tRNA, the EctRNA Leu/CUA  , and 
       the 3' flanking sequence of the SUP4 suppressor tRNA from plasmid
       pLeuRSB8T252A

<400>  20
caaagcttct ctttttcaat tgtatatgtg                                        30


<210>  21
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify a gene cassette containing the 5' flanking
       sequence of the SUP4 suppressor tRNA, the EctRNA Leu/CUA  , and 
       the 3' flanking sequence of the SUP4 suppressor tRNA from plasmid
       pLeuRSB8T252A

<400>  21
gagtcgacag acataaaaaa caaaaaaata c                                      31


<210>  22
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify the E. coli LeuRS gene

<400>  22
agctcgagtt agccaacgac cagattgag                                         29


<210>  23
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify the E. coli LeuRS gene

<400>  23
agactagtat gcaagagcaa taccgcccg                                         29


<210>  24
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify the SNR52 promoter from pSNR-TyrRS

<400>  24
ctaccgattc caccatccgg gcgatcattt atctttcact gcgg                        44


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify the  EctRNA Leu/CUA 3' flanking sequence 
       fragment from pLeuRSB8T252A

<400>  25
gcccggatgg tggaatcggt ag                                                22


<210>  26
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used toamplify a mutant GFP-TAG gene

<400>  26
tagtcggatc ctcagtgatg gtgatggtga tgcttgtaca gctcgtccat gcc              53


<210>  27
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used toamplify a mutant GFP-TAG gene

<400>  27
tagtcgtcga catggattac aaagatgatg atgataaagt gagcaagggc gaggag           56


<210>  28
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe  specific for the E. coli tRNATyr and the  EctRNA Tyr/CUA

<400>  28
tctgctccct ttggccgctc gggaacccc                                         29


<210>  29
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify a gene cassette containing ~200 bp 
       upstream of UPF1, the Kan-MX6, and ~200 bp downstream of UPF1

<400>  29
aatgaaaagc ttaccagaaa cttacg                                            26


<210>  30
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify a gene cassette containing ~200 bp 
       upstream of UPF1, the Kan-MX6, and ~200 bp downstream of UPF1

<400>  30
ggctaggata tcaagtccat gcc                                               23


<210>  31
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify genomic DNA ~300 bp away from the UPF1 
       gene

<400>  31
gatttgggag ggacaccttt atacgc                                            26


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer used to amplify genomic DNA ~300 bp away from the UPF1 
       gene

<400>  32
ttcattagaa gtacaatggt agccc                                             25


<210>  33
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid sequence encoding O-EctRNA Tyr/CUA

<400>  33
ggtggggttc ccgagcggcc aaagggagca gactctaaat ctgccgtcat cgacttcgaa       60

ggttcgaatc cttcccccac cacca                                             85


<210>  34
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid sequence encoding O-EctRNA Leu/CUA

<400>  34
gcccggatgg tggaatcggt agacacaagg gattctaaat ccctcggcgt tcgcgctgtg       60

cgggttcaag tcccgctccg ggtacca                                           87


<210>  35
<211>  1287
<212>  DNA
<213>  Escherichia coli

<400>  35
atggagattt tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta       60

gcccaggtga cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc      120

tattgcggct tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta      180

tgcctgaaac gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg      240

ggtctgattg gcgacccgag cttcaaagct gccgagcgta agctgaacac cgaagaaact      300

gttcaggagt gggtggacaa aatccgcaag caggttgccc cgttcctcga tttcgactgt      360

ggagaaaact ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg      420

accttcctgc gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg      480

gttaagcagc gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac      540

ctgttgcagg gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt      600

ggtggttctg atcagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat      660

cagaatcagg tgtttggcct gaccgttccg ctgatcacca aagcagatgg caccaaattt      720

ggtaaaactg aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc      780

taccagttct ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc      840

tttatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca      900

ccgcgcgccc agtatgtact ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt      960

ttacaggcgg caaaacgtat taccgaatgc ctgttcagcg gttctttgag tgcgctgagt     1020

gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc     1080

gcagacctga tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt     1140

aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac     1200

ttctttaaag aagaagatcg tctgtttggt cgttttacct tactgcgtcg cggcaaaaag     1260

aattactgtc tgatttgctg gaaataa                                         1287


<210>  36
<211>  428
<212>  PRT
<213>  Escherichia coli

<400>  36

Met Glu Ile Leu Met Ala Ser Ser Asn Leu Ile Lys Gln Leu Gln Glu 
1               5                   10                  15      


Arg Gly Leu Val Ala Gln Val Thr Asp Glu Glu Ala Leu Ala Glu Arg 
            20                  25                  30          


Leu Ala Gln Gly Pro Ile Ala Leu Tyr Cys Gly Phe Asp Pro Thr Ala 
        35                  40                  45              


Asp Ser Leu His Leu Gly His Leu Val Pro Leu Leu Cys Leu Lys Arg 
    50                  55                  60                  


Phe Gln Gln Ala Gly His Lys Pro Val Ala Leu Val Gly Gly Ala Thr 
65                  70                  75                  80  


Gly Leu Ile Gly Asp Pro Ser Phe Lys Ala Ala Glu Arg Lys Leu Asn 
                85                  90                  95      


Thr Glu Glu Thr Val Gln Glu Trp Val Asp Lys Ile Arg Lys Gln Val 
            100                 105                 110         


Ala Pro Phe Leu Asp Phe Asp Cys Gly Glu Asn Ser Ala Ile Ala Ala 
        115                 120                 125             


Asn Asn Tyr Asp Trp Phe Gly Asn Met Asn Val Leu Thr Phe Leu Arg 
    130                 135                 140                 


Asp Ile Gly Lys His Phe Ser Val Asn Gln Met Ile Asn Lys Glu Ala 
145                 150                 155                 160 


Val Lys Gln Arg Leu Asn Arg Glu Asp Gln Gly Ile Ser Phe Thr Glu 
                165                 170                 175     


Phe Ser Tyr Asn Leu Leu Gln Gly Tyr Asp Phe Ala Cys Leu Asn Lys 
            180                 185                 190         


Gln Tyr Gly Val Val Leu Gln Ile Gly Gly Ser Asp Gln Trp Gly Asn 
        195                 200                 205             


Ile Thr Ser Gly Ile Asp Leu Thr Arg Arg Leu His Gln Asn Gln Val 
    210                 215                 220                 


Phe Gly Leu Thr Val Pro Leu Ile Thr Lys Ala Asp Gly Thr Lys Phe 
225                 230                 235                 240 


Gly Lys Thr Glu Gly Gly Ala Val Trp Leu Asp Pro Lys Lys Thr Ser 
                245                 250                 255     


Pro Tyr Lys Phe Tyr Gln Phe Trp Ile Asn Thr Ala Asp Ala Asp Val 
            260                 265                 270         


Tyr Arg Phe Leu Lys Phe Phe Thr Phe Met Ser Ile Glu Glu Ile Asn 
        275                 280                 285             


Ala Leu Glu Glu Glu Asp Lys Asn Ser Gly Lys Ala Pro Arg Ala Gln 
    290                 295                 300                 


Tyr Val Leu Ala Glu Gln Val Thr Arg Leu Val His Gly Glu Glu Gly 
305                 310                 315                 320 


Leu Gln Ala Ala Lys Arg Ile Thr Glu Cys Leu Phe Ser Gly Ser Leu 
                325                 330                 335     


Ser Ala Leu Ser Glu Ala Asp Phe Glu Gln Leu Ala Gln Asp Gly Val 
            340                 345                 350         


Pro Met Val Glu Met Glu Lys Gly Ala Asp Leu Met Gln Ala Leu Val 
        355                 360                 365             


Asp Ser Glu Leu Gln Pro Ser Arg Gly Gln Ala Arg Lys Thr Ile Ala 
    370                 375                 380                 


Ser Asn Ala Ile Thr Ile Asn Gly Glu Lys Gln Ser Asp Pro Glu Tyr 
385                 390                 395                 400 


Phe Phe Lys Glu Glu Asp Arg Leu Phe Gly Arg Phe Thr Leu Leu Arg 
                405                 410                 415     


Arg Gly Lys Lys Asn Tyr Cys Leu Ile Cys Trp Lys 
            420                 425             


<210>  37
<211>  2583
<212>  DNA
<213>  Escherichia coli

<400>  37
atgcaagagc aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag       60

aagcgcacat ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg      120

cttccctatc cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac      180

gtgatcgccc gttaccagcg catgctgggc aaaaacgttc tgcagccgat cggctgggac      240

gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg      300

acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac      360

tggagccgcg agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc      420

accgagctgt ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg      480

aacgaccaga ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat      540

accaaagttg aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac      600

gagctgctca acgatttgga taaactggat cactggccag acaccgttaa aaccatgcag      660

cgtaactgga tcggtcgttc cgaaggcgtg gaaatcacct tcaacgttaa cgactatgac      720

aacacgctga ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg      780

gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc      840

tttattgacg aatgccgtaa caccaaagtt gctgaagctg aaatggcgac gatggagaaa      900

aaaggcgtca atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt      960

tgggcagcaa acttcgtatt gatggagtac ggcacgggcg cagtaatggc ggttccgggg     1020

cacgaccaac gcgactacga gtttgcctct aaatacggcc tgaacatcaa accggttatc     1080

ctggcggctg acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg     1140

ctgttcaact ctggcgagtt caacggtctt gaccatgaag cggccttcaa cgccatcgcc     1200

gataaactga ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgtgactgg     1260

ggtgtttctc gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt     1320

accgtaatgc cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg     1380

gacggcatta ccagcccgat taaagcagat ccacagtggg cgaaaactac cgttaacggt     1440

atgccagcgc tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat     1500

gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac     1560

tggctgccgg tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac     1620

ttccgcttct tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg     1680

aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac     1740

ggcgaacgta actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt     1800

atcgtgaaag cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg     1860

tccaagtcga agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac     1920

accgttcgtc tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa     1980

tccggcgtgg aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt gtacgagcat     2040

actgcgaaag gtgatgttgc ggcactgaac gttgatgcac tgactgaaga tcagaaagcg     2100

ctgcgtcgcg atgtgcataa aactatcgct aaagtgaccg atgatatcgg ccgtcgtcag     2160

accttcaaca ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcaccg     2220

accgatggcg agcaggatcg cgctctgatg caggaagcac tgctggccgt tgtccgtatg     2280

cttaacccgt tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc     2340

gatatcgaca acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg     2400

ctggtcgtgg tgcaggttaa cggtaaagtc cgtgctaaaa tcaccgttcc ggtggacgca     2460

acggaagaac aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat     2520

ggcgttactg tacgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc     2580

taa                                                                   2583


<210>  38
<211>  860
<212>  PRT
<213>  Escherichia coli

<400>  38

Met Gln Glu Gln Tyr Arg Pro Glu Glu Ile Glu Ser Lys Val Gln Leu 
1               5                   10                  15      


His Trp Asp Glu Lys Arg Thr Phe Glu Val Thr Glu Asp Glu Ser Lys 
            20                  25                  30          


Glu Lys Tyr Tyr Cys Leu Ser Met Leu Pro Tyr Pro Ser Gly Arg Leu 
        35                  40                  45              


His Met Gly His Val Arg Asn Tyr Thr Ile Gly Asp Val Ile Ala Arg 
    50                  55                  60                  


Tyr Gln Arg Met Leu Gly Lys Asn Val Leu Gln Pro Ile Gly Trp Asp 
65                  70                  75                  80  


Ala Phe Gly Leu Pro Ala Glu Gly Ala Ala Val Lys Asn Asn Thr Ala 
                85                  90                  95      


Pro Ala Pro Trp Thr Tyr Asp Asn Ile Ala Tyr Met Lys Asn Gln Leu 
            100                 105                 110         


Lys Met Leu Gly Phe Gly Tyr Asp Trp Ser Arg Glu Leu Ala Thr Cys 
        115                 120                 125             


Thr Pro Glu Tyr Tyr Arg Trp Glu Gln Lys Phe Phe Thr Glu Leu Tyr 
    130                 135                 140                 


Lys Lys Gly Leu Val Tyr Lys Lys Thr Ser Ala Val Asn Trp Cys Pro 
145                 150                 155                 160 


Asn Asp Gln Thr Val Leu Ala Asn Glu Gln Val Ile Asp Gly Cys Cys 
                165                 170                 175     


Trp Arg Cys Asp Thr Lys Val Glu Arg Lys Glu Ile Pro Gln Trp Phe 
            180                 185                 190         


Ile Lys Ile Thr Ala Tyr Ala Asp Glu Leu Leu Asn Asp Leu Asp Lys 
        195                 200                 205             


Leu Asp His Trp Pro Asp Thr Val Lys Thr Met Gln Arg Asn Trp Ile 
    210                 215                 220                 


Gly Arg Ser Glu Gly Val Glu Ile Thr Phe Asn Val Asn Asp Tyr Asp 
225                 230                 235                 240 


Asn Thr Leu Thr Val Tyr Thr Thr Arg Pro Asp Thr Phe Met Gly Cys 
                245                 250                 255     


Thr Tyr Leu Ala Val Ala Ala Gly His Pro Leu Ala Gln Lys Ala Ala 
            260                 265                 270         


Glu Asn Asn Pro Glu Leu Ala Ala Phe Ile Asp Glu Cys Arg Asn Thr 
        275                 280                 285             


Lys Val Ala Glu Ala Glu Met Ala Thr Met Glu Lys Lys Gly Val Asn 
    290                 295                 300                 


Thr Gly Phe Lys Ala Val His Pro Leu Thr Gly Glu Glu Ile Pro Val 
305                 310                 315                 320 


Trp Ala Ala Asn Phe Val Leu Met Glu Tyr Gly Thr Gly Ala Val Met 
                325                 330                 335     


Ala Val Pro Gly His Asp Gln Arg Asp Tyr Glu Phe Ala Ser Lys Tyr 
            340                 345                 350         


Gly Leu Asn Ile Lys Pro Val Ile Leu Ala Ala Asp Gly Ser Glu Pro 
        355                 360                 365             


Asp Leu Ser Gln Gln Ala Leu Thr Glu Lys Gly Val Leu Phe Asn Ser 
    370                 375                 380                 


Gly Glu Phe Asn Gly Leu Asp His Glu Ala Ala Phe Asn Ala Ile Ala 
385                 390                 395                 400 


Asp Lys Leu Thr Ala Met Gly Val Gly Glu Arg Lys Val Asn Tyr Arg 
                405                 410                 415     


Leu Arg Asp Trp Gly Val Ser Arg Gln Arg Tyr Trp Gly Ala Pro Ile 
            420                 425                 430         


Pro Met Val Thr Leu Glu Asp Gly Thr Val Met Pro Thr Pro Asp Asp 
        435                 440                 445             


Gln Leu Pro Val Ile Leu Pro Glu Asp Val Val Met Asp Gly Ile Thr 
    450                 455                 460                 


Ser Pro Ile Lys Ala Asp Pro Gln Trp Ala Lys Thr Thr Val Asn Gly 
465                 470                 475                 480 


Met Pro Ala Leu Arg Glu Thr Asp Thr Phe Asp Thr Phe Met Glu Ser 
                485                 490                 495     


Ser Trp Tyr Tyr Ala Arg Tyr Thr Cys Pro Glu Tyr Lys Glu Gly Met 
            500                 505                 510         


Leu Asp Ser Glu Ala Ala Asn Tyr Trp Leu Pro Val Asp Ile Tyr Ile 
        515                 520                 525             


Gly Gly Ile Glu His Ala Ile Met His Leu Leu Tyr Phe Arg Phe Phe 
    530                 535                 540                 


His Lys Leu Met Arg Asp Ala Gly Met Val Asn Ser Asp Glu Pro Ala 
545                 550                 555                 560 


Lys Gln Leu Leu Cys Gln Gly Met Val Leu Ala Asp Ala Phe Tyr Tyr 
                565                 570                 575     


Val Gly Glu Asn Gly Glu Arg Asn Trp Val Ser Pro Val Asp Ala Ile 
            580                 585                 590         


Val Glu Arg Asp Glu Lys Gly Arg Ile Val Lys Ala Lys Asp Ala Ala 
        595                 600                 605             


Gly His Glu Leu Val Tyr Thr Gly Met Ser Lys Met Ser Lys Ser Lys 
    610                 615                 620                 


Asn Asn Gly Ile Asp Pro Gln Val Met Val Glu Arg Tyr Gly Ala Asp 
625                 630                 635                 640 


Thr Val Arg Leu Phe Met Met Phe Ala Ser Pro Ala Asp Met Thr Leu 
                645                 650                 655     


Glu Trp Gln Glu Ser Gly Val Glu Gly Ala Asn Arg Phe Leu Lys Arg 
            660                 665                 670         


Val Trp Lys Leu Val Tyr Glu His Thr Ala Lys Gly Asp Val Ala Ala 
        675                 680                 685             


Leu Asn Val Asp Ala Leu Thr Glu Asp Gln Lys Ala Leu Arg Arg Asp 
    690                 695                 700                 


Val His Lys Thr Ile Ala Lys Val Thr Asp Asp Ile Gly Arg Arg Gln 
705                 710                 715                 720 


Thr Phe Asn Thr Ala Ile Ala Ala Ile Met Glu Leu Met Asn Lys Leu 
                725                 730                 735     


Ala Lys Ala Pro Thr Asp Gly Glu Gln Asp Arg Ala Leu Met Gln Glu 
            740                 745                 750         


Ala Leu Leu Ala Val Val Arg Met Leu Asn Pro Phe Thr Pro His Ile 
        755                 760                 765             


Cys Phe Thr Leu Trp Gln Glu Leu Lys Gly Glu Gly Asp Ile Asp Asn 
    770                 775                 780                 


Ala Pro Trp Pro Val Ala Asp Glu Lys Ala Met Val Glu Asp Ser Thr 
785                 790                 795                 800 


Leu Val Val Val Gln Val Asn Gly Lys Val Arg Ala Lys Ile Thr Val 
                805                 810                 815     


Pro Val Asp Ala Thr Glu Glu Gln Val Arg Glu Arg Ala Gly Gln Glu 
            820                 825                 830         


His Leu Val Ala Lys Tyr Leu Asp Gly Val Thr Val Arg Lys Val Ile 
        835                 840                 845             


Tyr Val Pro Gly Lys Leu Leu Asn Leu Val Val Gly 
    850                 855                 860 


<210>  39
<211>  1665
<212>  DNA
<213>  Escherichia coli

<400>  39
atgagtgagg cagaagcccg cccgactaac tttatccgtc agatcatcga tgaagatctg       60

gccagtggta agcacaccac agtacacacc cgtttcccgc cggagccgaa tggctatctg      120

catattggcc atgcgaaatc tatctgcctg aacttcggga tcgcccagga ctataaaggc      180

cagtgcaacc tgcgtttcga cgacactaac ccggtaaaag aagatatcga gtatgtcgat      240

tcgatcaaaa acgacgttga gtggttaggt tttcactggt ctggtaacgt ccgttactcc      300

tccgattatt ttgatcagct ccacgcctat gcgatcgaac tgatcaataa aggcctggcg      360

tacgttgatg aactgacgcc ggaacagatc cgcgaatacc gcggcaccct gacgcagcct      420

ggtaaaaaca gcccgtaccg cgaccgtagc gttgaagaga acctggcgct gttcgagaaa      480

atgcgtaccg gcggttttga agaaggtaaa gcctgcctgc gtgcgaaaat cgacatggct      540

tcaccgttta tcgtgatgcg cgatccggtg ctgtaccgca ttaaatttgc tgaacaccac      600

cagactggca acaagtggtg catctacccg atgtacgact tcacccactg catcagcgat      660

gccctggaag gtattacgca ctctctgtgt acgcttgagt tccaggataa ccgtcgtctg      720

tacgactggg tactggacaa catcaccatt cctgttcacc cgcgccagta cgaattctcg      780

cgcctgaatc tggaatacac tgtgatgtcc aagcgtaagc tgaacttgct ggtgaccgac      840

aagcacgttg aaggctggga tgacccgcgt atgccgacca tttccggtct gcgtcgtcgt      900

ggttacactg cggcttctat tcgtgagttc tgcaaacgca tcggcgtgac caagcaggac      960

aacaccattg agatggcgtc gctggaatcc tgcatccgtg aagatctcaa cgaaaatgcg     1020

ccgcgcgcaa tggcggttat cgatccggtg aaactggtta tcgaaaacta tcagggcgaa     1080

ggcgaaatgg ttaccatgcc gaaccatccg aacaaaccgg aaatggggag ccgtcaggtg     1140

ccgtttagcg gtgagatttg gattgatcgc tccgatttcc gcgaagaagc taacaagcag     1200

tacaaacgtc tggtgctggg taaagaagtg cgtctgcgta atgcttatgt gattaaggct     1260

gaacgcgtcg agaaagatgc cgaaggtaat atcaccacca tcttctgtac ttatgacgcc     1320

gataccttaa gcaaagatcc ggcagatggt cgtaaagtca aaggtgttat tcactgggtg     1380

agcgcggcac atgcgctgcc ggttgaaatc cgtttgtatg accgtctgtt cagcgtgccg     1440

aacccaggtg ctgcggatga tttcctgtcg gtgattaacc cggaatcgct ggtgatcaaa     1500

cagggctttg ctgaaccgtc gctgaaagat gcggttgcgg gtaaagcatt ccagtttgag     1560

cgtgaaggtt acttctgcct cgatagccgc cattctacgg cggaaaaacc ggtatttaac     1620

cgcaccgttg ggctgcgtga tacctgggcg aaagtaggcg agtaa                     1665


<210>  40
<211>  554
<212>  PRT
<213>  Escherichia coli

<400>  40

Met Ser Glu Ala Glu Ala Arg Pro Thr Asn Phe Ile Arg Gln Ile Ile 
1               5                   10                  15      


Asp Glu Asp Leu Ala Ser Gly Lys His Thr Thr Val His Thr Arg Phe 
            20                  25                  30          


Pro Pro Glu Pro Asn Gly Tyr Leu His Ile Gly His Ala Lys Ser Ile 
        35                  40                  45              


Cys Leu Asn Phe Gly Ile Ala Gln Asp Tyr Lys Gly Gln Cys Asn Leu 
    50                  55                  60                  


Arg Phe Asp Asp Thr Asn Pro Val Lys Glu Asp Ile Glu Tyr Val Asp 
65                  70                  75                  80  


Ser Ile Lys Asn Asp Val Glu Trp Leu Gly Phe His Trp Ser Gly Asn 
                85                  90                  95      


Val Arg Tyr Ser Ser Asp Tyr Phe Asp Gln Leu His Ala Tyr Ala Ile 
            100                 105                 110         


Glu Leu Ile Asn Lys Gly Leu Ala Tyr Val Asp Glu Leu Thr Pro Glu 
        115                 120                 125             


Gln Ile Arg Glu Tyr Arg Gly Thr Leu Thr Gln Pro Gly Lys Asn Ser 
    130                 135                 140                 


Pro Tyr Arg Asp Arg Ser Val Glu Glu Asn Leu Ala Leu Phe Glu Lys 
145                 150                 155                 160 


Met Arg Thr Gly Gly Phe Glu Glu Gly Lys Ala Cys Leu Arg Ala Lys 
                165                 170                 175     


Ile Asp Met Ala Ser Pro Phe Ile Val Met Arg Asp Pro Val Leu Tyr 
            180                 185                 190         


Arg Ile Lys Phe Ala Glu His His Gln Thr Gly Asn Lys Trp Cys Ile 
        195                 200                 205             


Tyr Pro Met Tyr Asp Phe Thr His Cys Ile Ser Asp Ala Leu Glu Gly 
    210                 215                 220                 


Ile Thr His Ser Leu Cys Thr Leu Glu Phe Gln Asp Asn Arg Arg Leu 
225                 230                 235                 240 


Tyr Asp Trp Val Leu Asp Asn Ile Thr Ile Pro Val His Pro Arg Gln 
                245                 250                 255     


Tyr Glu Phe Ser Arg Leu Asn Leu Glu Tyr Thr Val Met Ser Lys Arg 
            260                 265                 270         


Lys Leu Asn Leu Leu Val Thr Asp Lys His Val Glu Gly Trp Asp Asp 
        275                 280                 285             


Pro Arg Met Pro Thr Ile Ser Gly Leu Arg Arg Arg Gly Tyr Thr Ala 
    290                 295                 300                 


Ala Ser Ile Arg Glu Phe Cys Lys Arg Ile Gly Val Thr Lys Gln Asp 
305                 310                 315                 320 


Asn Thr Ile Glu Met Ala Ser Leu Glu Ser Cys Ile Arg Glu Asp Leu 
                325                 330                 335     


Asn Glu Asn Ala Pro Arg Ala Met Ala Val Ile Asp Pro Val Lys Leu 
            340                 345                 350         


Val Ile Glu Asn Tyr Gln Gly Glu Gly Glu Met Val Thr Met Pro Asn 
        355                 360                 365             


His Pro Asn Lys Pro Glu Met Gly Ser Arg Gln Val Pro Phe Ser Gly 
    370                 375                 380                 


Glu Ile Trp Ile Asp Arg Ser Asp Phe Arg Glu Glu Ala Asn Lys Gln 
385                 390                 395                 400 


Tyr Lys Arg Leu Val Leu Gly Lys Glu Val Arg Leu Arg Asn Ala Tyr 
                405                 410                 415     


Val Ile Lys Ala Glu Arg Val Glu Lys Asp Ala Glu Gly Asn Ile Thr 
            420                 425                 430         


Thr Ile Phe Cys Thr Tyr Asp Ala Asp Thr Leu Ser Lys Asp Pro Ala 
        435                 440                 445             


Asp Gly Arg Lys Val Lys Gly Val Ile His Trp Val Ser Ala Ala His 
    450                 455                 460                 


Ala Leu Pro Val Glu Ile Arg Leu Tyr Asp Arg Leu Phe Ser Val Pro 
465                 470                 475                 480 


Asn Pro Gly Ala Ala Asp Asp Phe Leu Ser Val Ile Asn Pro Glu Ser 
                485                 490                 495     


Leu Val Ile Lys Gln Gly Phe Ala Glu Pro Ser Leu Lys Asp Ala Val 
            500                 505                 510         


Ala Gly Lys Ala Phe Gln Phe Glu Arg Glu Gly Tyr Phe Cys Leu Asp 
        515                 520                 525             


Ser Arg His Ser Thr Ala Glu Lys Pro Val Phe Asn Arg Thr Val Gly 
    530                 535                 540                 


Leu Arg Asp Thr Trp Ala Lys Val Gly Glu 
545                 550                 


<210>  41
<211>  1275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant E. coli TyrRS EBzoRS encoding Y37G, D182G, L186A, and 
       D265R substitutions

<400>  41
atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg       60

gacgaggaag cgttagcaga gcgactggcg caaggcccga tcgcgctcgg ctgcggcttc      120

gatcctaccg ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc      180

ttccagcagg cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc      240

gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg      300

gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct      360

gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc      420

gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt      480

ctcaaccgtg aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt      540

tatggcttcg cctgtgccaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac      600

cagtggggta acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg      660

tttggcctga ccgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa      720

ggcggcgcag tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg      780

atcaacactg cgagagccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt      840

gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag      900

tatgtactgg cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca      960

aaacgtatta ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc     1020

gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg     1080

caggcactgg tcgattctga actgcaacct tcccgtggtc aggcacgtaa aactatcgcc     1140

tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa     1200

gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg     1260

atttgctgga aataa                                                      1275


<210>  42
<211>  1275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant E. coli TyrRS EAziRS encoding Y37L, D182S, F183A, L186A, 
       and D265R substitutions.

<400>  42
atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg       60

gacgaggaag cgttagcaga gcgactggcg caaggcccga tcgcgctcct gtgcggcttc      120

gatcctaccg ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc      180

ttccagcagg cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc      240

gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg      300

gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct      360

gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc      420

gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt      480

ctcaaccgtg aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt      540

tatagcgccg cctgtgccaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac      600

cagtggggta acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg      660

tttggcctga ccgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa      720

ggcggcgcag tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg      780

atcaacactg cgagagccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt      840

gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag      900

tatgtactgg cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca      960

aaacgtatta ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc     1020

gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg     1080

caggcactgg tcgattctga actgcaacct tcccgtggtc aggcacgtaa aactatcgcc     1140

tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa     1200

gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg     1260

atttgctgga aataa                                                      1275


<210>  43
<211>  1275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant E. coli TyrRS EOmeRS encoding Y37T, D182T, L183M, and 
       D265R substitutions

<400>  43
atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg       60

gacgaggaag cgttagcaga gcgactggcg caaggcccga tcgcgctcac ctgcggcttc      120

gatcctaccg ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc      180

ttccagcagg cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc      240

gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg      300

gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct      360

gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc      420

gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt      480

ctcaaccgtg aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt      540

tataccatgg cctgtctgaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac      600

cagtggggta acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg      660

tttggcctga ccgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa      720

ggcggcgcag tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg      780

atcaacactg cgagagccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt      840

gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag      900

tatgtactgg cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca      960

aaacgtatta ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc     1020

gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg     1080

caggcactgg tcgattctga actgcaacct tcccgtggtc aggcacgtaa aactatcgcc     1140

tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa     1200

gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg     1260

atttgctgga aataa                                                      1275


<210>  44
<211>  1275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant E. coli TyrRS EPyoRS encoding Y37G, D182S, F183M, and 
       D265R substitutions

<400>  44
atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg       60

gacgaggaag cgttagcaga gcgactggcg caaggcccga tcgcgctcgg ctgcggcttc      120

gatcctaccg ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc      180

ttccagcagg cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc      240

gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg      300

gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct      360

gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc      420

gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt      480

ctcaaccgtg aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt      540

tatagcatgg cctgtctgaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac      600

cagtggggta acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg      660

tttggcctga ccgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa      720

ggcggcgcag tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg      780

atcaacactg cgagagccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt      840

gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag      900

tatgtactgg cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca      960

aaacgtatta ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc     1020

gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg     1080

caggcactgg tcgattctga actgcaacct tcccgtggtc aggcacgtaa aactatcgcc     1140

tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa     1200

gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg     1260

atttgctgga aataa                                                      1275


<210>  45
<211>  1275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant E. coli TyrRS EKetRS encoding Y37I, D182G, F183M, L186A, 
       and D265R substitutions

<400>  45
atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg       60

gacgaggaag cgttagcaga gcgactggcg caaggcccga tcgcgctcat ctgcggcttc      120

gatcctaccg ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc      180

ttccagcagg cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc      240

gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg      300

gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct      360

gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc      420

gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt      480

ctcaaccgtg aaggccaggg gatttcgttc actgagtttt cctacaacct gttgcagggt      540

tatggcatgg cctgtgccaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac      600

cagtggggta acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg      660

tttggcctga ccgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa      720

ggcggcgcag tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg      780

atcaacactg cgagagccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt      840

gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag      900

tatgtactgg cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca      960

aaacgtatta ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc     1020

gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg     1080

caggcactgg tcgattctga actgcaacct tcccgtggtc aggcacgtaa aactatcgcc     1140

tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa     1200

gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg     1260

atttgctgga aataa                                                      1275


<210>  46
<211>  1275
<212>  DNA
<213>  Escherichia coli

<400>  46
atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg       60

gacgaggaag cgttagcaga gcgactggcg caaggcccga tcgcgctcta ttgcggcttc      120

gatcctaccg ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc      180

ttccagcagg cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc      240

gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg      300

gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct      360

gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc      420

gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt      480

ctcaaccgtg aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt      540

tatgacttcg cctgtctgaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac      600

cagtggggta acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg      660

tttggcctga ccgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa      720

ggcggcgcag tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg      780

atcaacactg cgagagccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt      840

gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag      900

tatgtactgg cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca      960

aaacgtatta ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc     1020

gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg     1080

caggcactgg tcgattctga actgcaacct tcccgtggtc aggcacgtaa aactatcgcc     1140

tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa     1200

gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg     1260

atttgctgga aataa                                                      1275


