                         SEQUENCE LISTING

<110>  E.I. duPont de Nemours & Company, Inc.
       Zhu, Quinn
 
<120>  IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH 
       EICOSAPENTAENOIC ACID PRODUCTION

<130>  CL4674

<150>  US 61/187366
<151>  2009-06-16

<150>  US 61/187368
<151>  2009-06-16

<150>  US 61/187359
<151>  2009-06-16

<160>  156   

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(24)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(24)

<400>  1

Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr Tyr Tyr 
1               5                   10                  15      


Asp Ser Leu Ala Val Met Gly Ser 
            20                  


<210>  2
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(19)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(19)

<400>  2

Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr Tyr Tyr Asp Ser 
1               5                   10                  15      


Leu Ala Val 
            


<210>  3
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(18)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(18)

<400>  3

Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr Tyr Tyr Asp Ser Leu 
1               5                   10                  15      


Ala Val 
        


<210>  4
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(18)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(18)

<400>  4

Pro Thr Arg Pro Ala Gly Pro Pro Pro Ala Thr Tyr Tyr Asp Ser Leu 
1               5                   10                  15      


Ala Val 
        


<210>  5
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(33)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(33)

<400>  5

Pro Gly Gly Pro Gly Lys Pro Ser Glu Ile Ala Ser Leu Pro Pro Pro 
1               5                   10                  15      


Ile Arg Pro Val Gly Asn Pro Pro Ala Ala Tyr Tyr Asp Ala Leu Ala 
            20                  25                  30          


Thr 
    


<210>  6
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(22)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(22)

<400>  6

Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr Tyr Tyr Asp Ser Leu 
1               5                   10                  15      


Ala Val Ser Gly Arg Thr 
            20          


<210>  7
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(36)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(36)

<400>  7

Pro Gly Gly Pro Gly Lys Pro Ser Glu Ile Ala Ser Leu Pro Pro Pro 
1               5                   10                  15      


Ile Arg Pro Val Gly Asn Pro Pro Ala Ala Tyr Tyr Asp Ala Leu Ala 
            20                  25                  30          


Thr Gly Arg Thr 
        35      


<210>  8
<211>  2112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multizyme: EgD9ES/EgD8M gene fusion


<220>
<221>  CDS
<222>  (1)..(2112)
<223>  Multizyme: EgD9ES/EgD8M gene fusion

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2112)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2112)

<400>  8
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag ggc gcc ggt ccc gct cga cct gcc gga ctt cct ccc gct acc        816
Ile Gln Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr           
            260                 265                 270                   

tac tac gac tct ctg gcc gtc atg gga tcc gtg aag gct tct cga cag        864
Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Val Lys Ala Ser Arg Gln           
        275                 280                 285                       

gct ctg ccc ctc gtc atc gac gga aag gtg tac gac gtc tcc gct tgg        912
Ala Leu Pro Leu Val Ile Asp Gly Lys Val Tyr Asp Val Ser Ala Trp           
    290                 295                 300                           

gtg aac ttc cac cct ggt gga gct gaa atc att gag aac tac cag gga        960
Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile Glu Asn Tyr Gln Gly           
305                 310                 315                 320           

cga gat gct act gac gcc ttc atg gtt atg cac tct cag gaa gcc ttc       1008
Arg Asp Ala Thr Asp Ala Phe Met Val Met His Ser Gln Glu Ala Phe           
                325                 330                 335               

gac aag ctc aag cga atg ccc aag atc aac cag gct tcc gag ctg cct       1056
Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Gln Ala Ser Glu Leu Pro           
            340                 345                 350                   

ccc cag gct gcc gtc aac gaa gct cag gag gat ttc cga aag ctc cga       1104
Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp Phe Arg Lys Leu Arg           
        355                 360                 365                       

gaa gag ctg atc gcc act ggc atg ttt gac gcc tct ccc ctc tgg tac       1152
Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala Ser Pro Leu Trp Tyr           
    370                 375                 380                           

tcg tac aag atc ttg acc acc ctg ggt ctt ggc gtg ctt gcc ttc ttc       1200
Ser Tyr Lys Ile Leu Thr Thr Leu Gly Leu Gly Val Leu Ala Phe Phe           
385                 390                 395                 400           

atg ctg gtc cag tac cac ctg tac ttc att ggt gct ctc gtg ctc ggt       1248
Met Leu Val Gln Tyr His Leu Tyr Phe Ile Gly Ala Leu Val Leu Gly           
                405                 410                 415               

atg cac tac cag caa atg gga tgg ctg tct cat gac atc tgc cac cac       1296
Met His Tyr Gln Gln Met Gly Trp Leu Ser His Asp Ile Cys His His           
            420                 425                 430                   

cag acc ttc aag aac cga aac tgg aat aac gtc ctg ggt ctg gtc ttt       1344
Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Val Leu Gly Leu Val Phe           
        435                 440                 445                       

ggc aac gga ctc cag ggc ttc tcc gtg acc tgg tgg aag gac aga cac       1392
Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Trp Trp Lys Asp Arg His           
    450                 455                 460                           

aac gcc cat cat tct gct acc aac gtt cag ggt cac gat ccc gac att       1440
Asn Ala His His Ser Ala Thr Asn Val Gln Gly His Asp Pro Asp Ile           
465                 470                 475                 480           

gat aac ctg cct ctg ctc gcc tgg tcc gag gac gat gtc act cga gct       1488
Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu Asp Asp Val Thr Arg Ala           
                485                 490                 495               

tct ccc atc tcc cga aag ctc att cag ttc caa cag tac tat ttc ctg       1536
Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln Gln Tyr Tyr Phe Leu           
            500                 505                 510                   

gtc atc tgt att ctc ctg cga ttc atc tgg tgt ttc cag tct gtg ctg       1584
Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys Phe Gln Ser Val Leu           
        515                 520                 525                       

acc gtt cga tcc ctc aag gac cga gac aac cag ttc tac cga tct cag       1632
Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln Phe Tyr Arg Ser Gln           
    530                 535                 540                           

tac aag aaa gag gcc att gga ctc gct ctg cac tgg act ctc aag acc       1680
Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His Trp Thr Leu Lys Thr           
545                 550                 555                 560           

ctg ttc cac ctc ttc ttt atg ccc tcc atc ctg acc tcg atg ctg gtg       1728
Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu Thr Ser Met Leu Val           
                565                 570                 575               

ttc ttt gtt tcc gag ctc gtc ggt ggc ttc gga att gcc atc gtg gtc       1776
Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly Ile Ala Ile Val Val           
            580                 585                 590                   

ttc atg aac cac tac cct ctg gag aag atc ggt gat tcc gtc tgg gac       1824
Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly Asp Ser Val Trp Asp           
        595                 600                 605                       

gga cat ggc ttc tct gtg ggt cag atc cat gag acc atg aac att cga       1872
Gly His Gly Phe Ser Val Gly Gln Ile His Glu Thr Met Asn Ile Arg           
    610                 615                 620                           

cga ggc atc att act gac tgg ttc ttt gga ggc ctg aac tac cag atc       1920
Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly Leu Asn Tyr Gln Ile           
625                 630                 635                 640           

gag cac cat ctc tgg ccc acc ctg cct cga cac aac ctc act gcc gtt       1968
Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn Leu Thr Ala Val           
                645                 650                 655               

tcc tac cag gtg gaa cag ctg tgc cag aag cac aac ctc ccc tac cga       2016
Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His Asn Leu Pro Tyr Arg           
            660                 665                 670                   

aac cct ctg ccc cat gaa ggt ctc gtc atc ctg ctc cga tac ctg tcc       2064
Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu Leu Arg Tyr Leu Ser           
        675                 680                 685                       

cag ttc gct cga atg gcc gag aag cag ccc ggt gcc aag gct cag taa       2112
Gln Phe Ala Arg Met Ala Glu Lys Gln Pro Gly Ala Lys Ala Gln               
    690                 695                 700                           


<210>  9
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  9

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr 
            260                 265                 270         


Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Val Lys Ala Ser Arg Gln 
        275                 280                 285             


Ala Leu Pro Leu Val Ile Asp Gly Lys Val Tyr Asp Val Ser Ala Trp 
    290                 295                 300                 


Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile Glu Asn Tyr Gln Gly 
305                 310                 315                 320 


Arg Asp Ala Thr Asp Ala Phe Met Val Met His Ser Gln Glu Ala Phe 
                325                 330                 335     


Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Gln Ala Ser Glu Leu Pro 
            340                 345                 350         


Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp Phe Arg Lys Leu Arg 
        355                 360                 365             


Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala Ser Pro Leu Trp Tyr 
    370                 375                 380                 


Ser Tyr Lys Ile Leu Thr Thr Leu Gly Leu Gly Val Leu Ala Phe Phe 
385                 390                 395                 400 


Met Leu Val Gln Tyr His Leu Tyr Phe Ile Gly Ala Leu Val Leu Gly 
                405                 410                 415     


Met His Tyr Gln Gln Met Gly Trp Leu Ser His Asp Ile Cys His His 
            420                 425                 430         


Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Val Leu Gly Leu Val Phe 
        435                 440                 445             


Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Trp Trp Lys Asp Arg His 
    450                 455                 460                 


Asn Ala His His Ser Ala Thr Asn Val Gln Gly His Asp Pro Asp Ile 
465                 470                 475                 480 


Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu Asp Asp Val Thr Arg Ala 
                485                 490                 495     


Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln Gln Tyr Tyr Phe Leu 
            500                 505                 510         


Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys Phe Gln Ser Val Leu 
        515                 520                 525             


Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln Phe Tyr Arg Ser Gln 
    530                 535                 540                 


Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His Trp Thr Leu Lys Thr 
545                 550                 555                 560 


Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu Thr Ser Met Leu Val 
                565                 570                 575     


Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly Ile Ala Ile Val Val 
            580                 585                 590         


Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly Asp Ser Val Trp Asp 
        595                 600                 605             


Gly His Gly Phe Ser Val Gly Gln Ile His Glu Thr Met Asn Ile Arg 
    610                 615                 620                 


Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly Leu Asn Tyr Gln Ile 
625                 630                 635                 640 


Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn Leu Thr Ala Val 
                645                 650                 655     


Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His Asn Leu Pro Tyr Arg 
            660                 665                 670         


Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu Leu Arg Tyr Leu Ser 
        675                 680                 685             


Gln Phe Ala Arg Met Ala Glu Lys Gln Pro Gly Ala Lys Ala Gln 
    690                 695                 700             


<210>  10
<211>  2109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multizyme: EaD9ES/EaD8S gene fusion


<220>
<221>  CDS
<222>  (1)..(2109)
<223>  Multizyme: EaD9ES/EaD8S gene fusion

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2109)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2109)

<400>  10
atg gag gct gcc aag gag ctg gtc tcc atc gtc cag gag gaa ctt ccc         48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro           
1               5                   10                  15                

aag gtg gac tac gcc cag ctc tgg cag gac gcc tcc tct tgc gag gtt         96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcg gtc gct ttc gtg gcc atc aag ttc atg ctt cga cct        144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro           
        35                  40                  45                        

ctg gac ctc aag cga caa gcc acc ctc aaa aag ctg ttc acc gca tac        192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr           
    50                  55                  60                            

aac ttt ctc atg tcc atc tac tcg ttc ggc tcc ttc ctg gcg atg gcc        240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala           
65                  70                  75                  80            

tac gct ctc tct gtc act ggt att ctt tcc ggc gat tgt gag act gcc        288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala           
                85                  90                  95                

ttc aac aat gac gtg ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc gtc gag tac atc gac tcc ttc tac ctt ccc ctc atg gac aag        384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys           
        115                 120                 125                       

ccc ttg tcg ttt ctg cag ttc ttt cac cat ctc gga gct ccc atc gac        432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aag tat cga aac gaa ggc gtc tgg atc ttt gtt        480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggt tac tat tgg acg        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctc atc aag ctg aac ttc cct atg ccc aag aac ctc att acc tcc        576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser           
            180                 185                 190                   

atg caa att atc cag ttc aac gtc gga ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cga aac gtg ccc tgc tac cgg cag gac ggt atg cga atg ttt gcc tgg        672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp           
    210                 215                 220                           

atc ttc aac tac tgg tat gtc ggc acg gtg ctg ctt ctg ttc ctc aac        720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtc cag acc tac att cgg aag cct cga aag aac cga ggc aaa        768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys           
                245                 250                 255               

aag gag ggc gcc ggt ccc gct cga cct gcc gga ctt cct ccc gct acc        816
Lys Glu Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr           
            260                 265                 270                   

tac tac gac tct ctg gcc gtc atg gga tcc atg gtc aag cga ccc gct        864
Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Met Val Lys Arg Pro Ala           
        275                 280                 285                       

ctg cct ctc acc gtg gac ggt gtc acc tac gac gtt tct gcc tgg ctc        912
Leu Pro Leu Thr Val Asp Gly Val Thr Tyr Asp Val Ser Ala Trp Leu           
    290                 295                 300                           

aac cac cat ccc gga ggt gcc gac att atc gag aac tac cga ggt cgg        960
Asn His His Pro Gly Gly Ala Asp Ile Ile Glu Asn Tyr Arg Gly Arg           
305                 310                 315                 320           

gat gct acc gac gtc ttc atg gtt atg cac tcc gag aac gcc gtg tcc       1008
Asp Ala Thr Asp Val Phe Met Val Met His Ser Glu Asn Ala Val Ser           
                325                 330                 335               

aaa ctc aga cga atg ccc atc atg gaa cct tcc tct ccc ctg act cca       1056
Lys Leu Arg Arg Met Pro Ile Met Glu Pro Ser Ser Pro Leu Thr Pro           
            340                 345                 350                   

aca cct ccc aag cca aac tcc gac gaa cct cag gag gat ttc cga aag       1104
Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro Gln Glu Asp Phe Arg Lys           
        355                 360                 365                       

ctg cga gac gag ctc att gct gca ggc atg ttc gat gcc tct ccc atg       1152
Leu Arg Asp Glu Leu Ile Ala Ala Gly Met Phe Asp Ala Ser Pro Met           
    370                 375                 380                           

tgg tac gct tac aag acc ctg tcg act ctc gga ctg ggt gtc ctt gcc       1200
Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu Gly Leu Gly Val Leu Ala           
385                 390                 395                 400           

gtg ctg ttg atg acc cag tgg cac tgg tac ctg gtt ggt gct atc gtc       1248
Val Leu Leu Met Thr Gln Trp His Trp Tyr Leu Val Gly Ala Ile Val           
                405                 410                 415               

ctc ggc att cac ttt caa cag atg gga tgg ctc tcg cac gac att tgc       1296
Leu Gly Ile His Phe Gln Gln Met Gly Trp Leu Ser His Asp Ile Cys           
            420                 425                 430                   

cat cac cag ctg ttc aag gac cga tcc atc aac aat gcc att ggc ctg       1344
His His Gln Leu Phe Lys Asp Arg Ser Ile Asn Asn Ala Ile Gly Leu           
        435                 440                 445                       

ctc ttc gga aac gtg ctt cag ggc ttt tct gtc act tgg tgg aag gac       1392
Leu Phe Gly Asn Val Leu Gln Gly Phe Ser Val Thr Trp Trp Lys Asp           
    450                 455                 460                           

cga cac aac gct cat cac tcc gcc acc aac gtg cag ggt cac gat ccc       1440
Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly His Asp Pro           
465                 470                 475                 480           

gac atc gac aac ctg cct ctc ctg gcg tgg tcc aag gag gac gtc gag       1488
Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Lys Glu Asp Val Glu           
                485                 490                 495               

cga gct ggc ccg ttt tct cga cgg atg atc aag tac caa cag tat tac       1536
Arg Ala Gly Pro Phe Ser Arg Arg Met Ile Lys Tyr Gln Gln Tyr Tyr           
            500                 505                 510                   

ttc ttt ttc atc tgt gcc ctt ctg cga ttc atc tgg tgc ttt cag tcc       1584
Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe Ile Trp Cys Phe Gln Ser           
        515                 520                 525                       

att cat act gcc acg ggt ctc aag gat cga agc aat cag tac tat cga       1632
Ile His Thr Ala Thr Gly Leu Lys Asp Arg Ser Asn Gln Tyr Tyr Arg           
    530                 535                 540                           

aga cag tac gag aag gag tcc gtc ggt ctg gca ctc cac tgg ggt ctc       1680
Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu Ala Leu His Trp Gly Leu           
545                 550                 555                 560           

aag gcc ttg ttc tac tat ttc tac atg ccc tcg ttt ctc acc gga ctc       1728
Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro Ser Phe Leu Thr Gly Leu           
                565                 570                 575               

atg gtg ttc ttt gtc tcc gag ctg ctt ggt ggc ttc gga att gcc atc       1776
Met Val Phe Phe Val Ser Glu Leu Leu Gly Gly Phe Gly Ile Ala Ile           
            580                 585                 590                   

gtt gtc ttc atg aac cac tac cct ctg gag aag att cag gac tcc gtg       1824
Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gln Asp Ser Val           
        595                 600                 605                       

tgg gat ggt cat ggc ttc tgt gct gga cag att cac gag acc atg aac       1872
Trp Asp Gly His Gly Phe Cys Ala Gly Gln Ile His Glu Thr Met Asn           
    610                 615                 620                           

gtt cag cga ggc ctc gtc aca gac tgg ttt ttc ggt ggc ctc aac tac       1920
Val Gln Arg Gly Leu Val Thr Asp Trp Phe Phe Gly Gly Leu Asn Tyr           
625                 630                 635                 640           

cag atc gaa cat cac ctg tgg cct act ctt ccc aga cac aac ctc acc       1968
Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn Leu Thr           
                645                 650                 655               

gct gcc tcc atc aaa gtg gag cag ctg tgc aag aag cac aac ctg ccc       2016
Ala Ala Ser Ile Lys Val Glu Gln Leu Cys Lys Lys His Asn Leu Pro           
            660                 665                 670                   

tac cga tct cct ccc atg ctc gaa ggt gtc ggc att ctt atc tcc tac       2064
Tyr Arg Ser Pro Pro Met Leu Glu Gly Val Gly Ile Leu Ile Ser Tyr           
        675                 680                 685                       

ctg ggc acc ttc gct cga atg gtt gcc aag gca gac aag gcc taa           2109
Leu Gly Thr Phe Ala Arg Met Val Ala Lys Ala Asp Lys Ala                   
    690                 695                 700                           


<210>  11
<211>  702
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr 
            260                 265                 270         


Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Met Val Lys Arg Pro Ala 
        275                 280                 285             


Leu Pro Leu Thr Val Asp Gly Val Thr Tyr Asp Val Ser Ala Trp Leu 
    290                 295                 300                 


Asn His His Pro Gly Gly Ala Asp Ile Ile Glu Asn Tyr Arg Gly Arg 
305                 310                 315                 320 


Asp Ala Thr Asp Val Phe Met Val Met His Ser Glu Asn Ala Val Ser 
                325                 330                 335     


Lys Leu Arg Arg Met Pro Ile Met Glu Pro Ser Ser Pro Leu Thr Pro 
            340                 345                 350         


Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro Gln Glu Asp Phe Arg Lys 
        355                 360                 365             


Leu Arg Asp Glu Leu Ile Ala Ala Gly Met Phe Asp Ala Ser Pro Met 
    370                 375                 380                 


Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu Gly Leu Gly Val Leu Ala 
385                 390                 395                 400 


Val Leu Leu Met Thr Gln Trp His Trp Tyr Leu Val Gly Ala Ile Val 
                405                 410                 415     


Leu Gly Ile His Phe Gln Gln Met Gly Trp Leu Ser His Asp Ile Cys 
            420                 425                 430         


His His Gln Leu Phe Lys Asp Arg Ser Ile Asn Asn Ala Ile Gly Leu 
        435                 440                 445             


Leu Phe Gly Asn Val Leu Gln Gly Phe Ser Val Thr Trp Trp Lys Asp 
    450                 455                 460                 


Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly His Asp Pro 
465                 470                 475                 480 


Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Lys Glu Asp Val Glu 
                485                 490                 495     


Arg Ala Gly Pro Phe Ser Arg Arg Met Ile Lys Tyr Gln Gln Tyr Tyr 
            500                 505                 510         


Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe Ile Trp Cys Phe Gln Ser 
        515                 520                 525             


Ile His Thr Ala Thr Gly Leu Lys Asp Arg Ser Asn Gln Tyr Tyr Arg 
    530                 535                 540                 


Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu Ala Leu His Trp Gly Leu 
545                 550                 555                 560 


Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro Ser Phe Leu Thr Gly Leu 
                565                 570                 575     


Met Val Phe Phe Val Ser Glu Leu Leu Gly Gly Phe Gly Ile Ala Ile 
            580                 585                 590         


Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gln Asp Ser Val 
        595                 600                 605             


Trp Asp Gly His Gly Phe Cys Ala Gly Gln Ile His Glu Thr Met Asn 
    610                 615                 620                 


Val Gln Arg Gly Leu Val Thr Asp Trp Phe Phe Gly Gly Leu Asn Tyr 
625                 630                 635                 640 


Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn Leu Thr 
                645                 650                 655     


Ala Ala Ser Ile Lys Val Glu Gln Leu Cys Lys Lys His Asn Leu Pro 
            660                 665                 670         


Tyr Arg Ser Pro Pro Met Leu Glu Gly Val Gly Ile Leu Ile Ser Tyr 
        675                 680                 685             


Leu Gly Thr Phe Ala Arg Met Val Ala Lys Ala Asp Lys Ala 
    690                 695                 700         


<210>  12
<211>  2127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multizyme: E389D9eS/EgD8M gene fusion


<220>
<221>  CDS
<222>  (1)..(2127)
<223>  Multizyme: E389D9eS/EgD8M gene fusion

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2127)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2127)

<400>  12
atg gct gcc gtc atc gag gtg gcc aac gag ttc gtc gct atc act gcc         48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala           
1               5                   10                  15                

gag acc ctt ccc aag gtg gac tat cag cga ctc tgg cga gac atc tac         96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr           
            20                  25                  30                    

tcc tgc gag ctc ctg tac ttc tcc att gct ttc gtc atc ctc aag ttt        144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe           
        35                  40                  45                        

acc ctt ggc gag ctc tcg gat tct ggc aaa aag att ctg cga gtg ctg        192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu           
    50                  55                  60                            

ttc aag tgg tac aac ctc ttc atg tcc gtc ttt tcg ctg gtg tcc ttc        240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe           
65                  70                  75                  80            

ctc tgt atg ggt tac gcc atc tac acc gtt gga ctg tac tcc aac gaa        288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu           
                85                  90                  95                

tgc gac aga gct ttc gac aac agc ttg ttc cga ttt gcc acc aag gtc        336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val           
            100                 105                 110                   

ttc tac tat tcc aag ttt ctg gag tac atc gac tct ttc tac ctt ccc        384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro           
        115                 120                 125                       

ctc atg gcc aag cct ctg tcc ttt ctg cag ttc ttt cat cac ttg gga        432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly           
    130                 135                 140                           

gct cct atg gac atg tgg ctc ttc gtg cag tac tct ggc gaa tcc att        480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile           
145                 150                 155                 160           

tgg atc ttt gtg ttc ctg aac gga ttc att cac ttt gtc atg tac ggc        528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly           
                165                 170                 175               

tac tat tgg aca cgg ctg atg aag ttc aac ttt ccc atg ccc aag cag        576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln           
            180                 185                 190                   

ctc att acc gca atg cag atc acc cag ttc aac gtt ggc ttc tac ctc        624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu           
        195                 200                 205                       

gtg tgg tgg tac aag gac att ccc tgt tac cga aag gat ccc atg cga        672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg           
    210                 215                 220                           

atg ctg gcc tgg atc ttc aac tac tgg tac gtc ggt acc gtt ctt ctg        720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu           
225                 230                 235                 240           

ctc ttc atc aac ttc ttt gtc aag tcc tac gtg ttt ccc aag cct aag        768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys           
                245                 250                 255               

act gcc gac aaa aag gtc cag ggc gcc ggt ccc gct cga cct gcc gga        816
Thr Ala Asp Lys Lys Val Gln Gly Ala Gly Pro Ala Arg Pro Ala Gly           
            260                 265                 270                   

ctt cct ccc gct acc tac tac gac tct ctg gcc gtc atg gga tcc gtg        864
Leu Pro Pro Ala Thr Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Val           
        275                 280                 285                       

aag gct tct cga cag gct ctg ccc ctc gtc atc gac gga aag gtg tac        912
Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys Val Tyr           
    290                 295                 300                           

gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa atc att        960
Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile           
305                 310                 315                 320           

gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt atg cac       1008
Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val Met His           
                325                 330                 335               

tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc aac cag       1056
Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Gln           
            340                 345                 350                   

gct tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag gag gat       1104
Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp           
        355                 360                 365                       

ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt gac gcc       1152
Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala           
    370                 375                 380                           

tct ccc ctc tgg tac tcg tac aag atc ttg acc acc ctg ggt ctt ggc       1200
Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly Leu Gly           
385                 390                 395                 400           

gtg ctt gcc ttc ttc atg ctg gtc cag tac cac ctg tac ttc att ggt       1248
Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe Ile Gly           
                405                 410                 415               

gct ctc gtg ctc ggt atg cac tac cag caa atg gga tgg ctg tct cat       1296
Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu Ser His           
            420                 425                 430                   

gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat aac gtc       1344
Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Val           
        435                 440                 445                       

ctg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg acc tgg       1392
Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Trp           
    450                 455                 460                           

tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt cag ggt       1440
Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly           
465                 470                 475                 480           

cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc gag gac       1488
His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu Asp           
                485                 490                 495               

gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag ttc caa       1536
Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln           
            500                 505                 510                   

cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc tgg tgt       1584
Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys           
        515                 520                 525                       

ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac aac cag       1632
Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln           
    530                 535                 540                           

ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct ctg cac       1680
Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His           
545                 550                 555                 560           

tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc atc ctg       1728
Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu           
                565                 570                 575               

acc tcg atg ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc ttc gga       1776
Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly           
            580                 585                 590                   

att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag atc ggt       1824
Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly           
        595                 600                 605                       

gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc cat gag       1872
Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His Glu           
    610                 615                 620                           

acc atg aac att cga cga ggc atc att act gac tgg ttc ttt gga ggc       1920
Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly           
625                 630                 635                 640           

ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct cga cac       1968
Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His           
                645                 650                 655               

aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag aag cac       2016
Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His           
            660                 665                 670                   

aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc atc ctg       2064
Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu           
        675                 680                 685                       

ctc cga tac ctg tcc cag ttc gct cga atg gcc gag aag cag ccc ggt       2112
Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln Pro Gly           
    690                 695                 700                           

gcc aag gct cag taa                                                   2127
Ala Lys Ala Gln                                                           
705                                                                       


<210>  13
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln Gly Ala Gly Pro Ala Arg Pro Ala Gly 
            260                 265                 270         


Leu Pro Pro Ala Thr Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Val 
        275                 280                 285             


Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys Val Tyr 
    290                 295                 300                 


Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile 
305                 310                 315                 320 


Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val Met His 
                325                 330                 335     


Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Gln 
            340                 345                 350         


Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp 
        355                 360                 365             


Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala 
    370                 375                 380                 


Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly Leu Gly 
385                 390                 395                 400 


Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe Ile Gly 
                405                 410                 415     


Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu Ser His 
            420                 425                 430         


Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Val 
        435                 440                 445             


Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Trp 
    450                 455                 460                 


Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly 
465                 470                 475                 480 


His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu Asp 
                485                 490                 495     


Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln 
            500                 505                 510         


Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys 
        515                 520                 525             


Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln 
    530                 535                 540                 


Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His 
545                 550                 555                 560 


Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu 
                565                 570                 575     


Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly 
            580                 585                 590         


Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly 
        595                 600                 605             


Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His Glu 
    610                 615                 620                 


Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly 
625                 630                 635                 640 


Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His 
                645                 650                 655     


Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His 
            660                 665                 670         


Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu 
        675                 680                 685             


Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln Pro Gly 
    690                 695                 700                 


Ala Lys Ala Gln 
705             


<210>  14
<211>  1860
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1860)
<223>  GenBank Accession No. NP_014818; "YOR175C"

<300>
<302>  Genes encoding a novel type of lysophophatidylcholine 
       acyltransferases and their use to increase triacylglycerol 
       production and/or modify fatty acid composition
<310>  US-2008-0145867-A1
<311>  2007-06-15
<312>  2008-06-19
<313>  (1)..(1860)

<300>
<302>  Genes encoding a novel type of lysophophatidylcholine 
       acyltransferases and their use to increase triacylglycerol 
       production and/or modify fatty acid composition
<310>  WO 2008/076377
<311>  2007-12-13
<312>  2008-06-26
<313>  (1)..(1860)

<300>
<302>  USE OF A CLASS OF GENES ENCODING LYSOPHOSPHOLIPID ACYL 
       TRANSFERASES FOR APPLICATION IN AGRICULTURE, BIOTECHNOLOGY AND 
       MEDICINE
<310>  WO 2009/001315
<311>  2008-06-25
<312>  2008-12-31
<313>  (1)..(1860)

<400>  14
atg tac aat cct gtg gac gct gtt tta aca aag ata att acc aac tat         48
Met Tyr Asn Pro Val Asp Ala Val Leu Thr Lys Ile Ile Thr Asn Tyr           
1               5                   10                  15                

ggg att gat agt ttt aca ctg cga tat gct atc tgc tta ttg gga tcg         96
Gly Ile Asp Ser Phe Thr Leu Arg Tyr Ala Ile Cys Leu Leu Gly Ser           
            20                  25                  30                    

ttc cca ctg aat gct att ttg aag aga att ccc gag aag cgt ata ggt        144
Phe Pro Leu Asn Ala Ile Leu Lys Arg Ile Pro Glu Lys Arg Ile Gly           
        35                  40                  45                        

tta aaa tgt tgt ttt atc att tct atg tcg atg ttt tac tta ttc ggt        192
Leu Lys Cys Cys Phe Ile Ile Ser Met Ser Met Phe Tyr Leu Phe Gly           
    50                  55                  60                            

gtg ctg aat cta gta agt gga ttc agg acc ctg ttt att agt acc atg        240
Val Leu Asn Leu Val Ser Gly Phe Arg Thr Leu Phe Ile Ser Thr Met           
65                  70                  75                  80            

ttt act tac ttg atc tca aga ttt tac cgt tcc aag ttt atg cca cac        288
Phe Thr Tyr Leu Ile Ser Arg Phe Tyr Arg Ser Lys Phe Met Pro His           
                85                  90                  95                

ttg aat ttc atg ttt gtt atg ggt cat ttg gca ata aat cat ata cac        336
Leu Asn Phe Met Phe Val Met Gly His Leu Ala Ile Asn His Ile His           
            100                 105                 110                   

gcc caa ttc ctt aac gaa cag act caa act acc gtt gac att aca agt        384
Ala Gln Phe Leu Asn Glu Gln Thr Gln Thr Thr Val Asp Ile Thr Ser           
        115                 120                 125                       

tca caa atg gtt tta gcc atg aaa cta act tct ttt gca tgg tcg tac        432
Ser Gln Met Val Leu Ala Met Lys Leu Thr Ser Phe Ala Trp Ser Tyr           
    130                 135                 140                           

tat gat ggt tca tgc act agc gaa agc gat ttc aaa gat ttg act gag        480
Tyr Asp Gly Ser Cys Thr Ser Glu Ser Asp Phe Lys Asp Leu Thr Glu           
145                 150                 155                 160           

cat caa aaa tct cgt gct gtc aga ggt cat cca ccc tta tta aag ttc        528
His Gln Lys Ser Arg Ala Val Arg Gly His Pro Pro Leu Leu Lys Phe           
                165                 170                 175               

ctg gca tat gca ttt ttc tat tca acg ttg cta act ggc cca agt ttc        576
Leu Ala Tyr Ala Phe Phe Tyr Ser Thr Leu Leu Thr Gly Pro Ser Phe           
            180                 185                 190                   

gat tat gcc gat ttt gac agc tgg ttg aat tgt gag atg ttc cgt gac        624
Asp Tyr Ala Asp Phe Asp Ser Trp Leu Asn Cys Glu Met Phe Arg Asp           
        195                 200                 205                       

ttg cct gaa agc aaa aag cct atg aga aga cac cac cct ggt gaa aga        672
Leu Pro Glu Ser Lys Lys Pro Met Arg Arg His His Pro Gly Glu Arg           
    210                 215                 220                           

aga cag att cca aag aat ggt aaa ctt gca tta tgg aaa gtt gtt caa        720
Arg Gln Ile Pro Lys Asn Gly Lys Leu Ala Leu Trp Lys Val Val Gln           
225                 230                 235                 240           

ggt ctt gct tgg atg att tta agt aca cta gga atg aag cac ttc ccc        768
Gly Leu Ala Trp Met Ile Leu Ser Thr Leu Gly Met Lys His Phe Pro           
                245                 250                 255               

gta aaa tac gtt ttg gac aaa gat ggc ttc cca acg aga tct ttt ata        816
Val Lys Tyr Val Leu Asp Lys Asp Gly Phe Pro Thr Arg Ser Phe Ile           
            260                 265                 270                   

ttc aga atc cat tac tta ttc ttg ctt ggt ttc atc cat aga ttc aag        864
Phe Arg Ile His Tyr Leu Phe Leu Leu Gly Phe Ile His Arg Phe Lys           
        275                 280                 285                       

tac tac gct gcc tgg act att tcg gaa gga tct tgt att ttg tgc ggt        912
Tyr Tyr Ala Ala Trp Thr Ile Ser Glu Gly Ser Cys Ile Leu Cys Gly           
    290                 295                 300                           

ttg ggt tat aat ggt tat gat tca aag aca caa aag atc aga tgg gat        960
Leu Gly Tyr Asn Gly Tyr Asp Ser Lys Thr Gln Lys Ile Arg Trp Asp           
305                 310                 315                 320           

cgt gtc aga aat att gac att tgg acc gta gaa acg gcg cag aat acg       1008
Arg Val Arg Asn Ile Asp Ile Trp Thr Val Glu Thr Ala Gln Asn Thr           
                325                 330                 335               

cgt gaa atg ttg gaa gca tgg aat atg aat act aac aag tgg cta aaa       1056
Arg Glu Met Leu Glu Ala Trp Asn Met Asn Thr Asn Lys Trp Leu Lys           
            340                 345                 350                   

tac tct gtt tat tta cgt gtc aca aag aag ggc aaa aaa cct ggt ttc       1104
Tyr Ser Val Tyr Leu Arg Val Thr Lys Lys Gly Lys Lys Pro Gly Phe           
        355                 360                 365                       

cgc tca act ttg ttt act ttc cta act tcc gca ttt tgg cat ggt acc       1152
Arg Ser Thr Leu Phe Thr Phe Leu Thr Ser Ala Phe Trp His Gly Thr           
    370                 375                 380                           

aga cct ggg tac tat ctg act ttt gcg aca ggg gct ttg tac caa aca       1200
Arg Pro Gly Tyr Tyr Leu Thr Phe Ala Thr Gly Ala Leu Tyr Gln Thr           
385                 390                 395                 400           

tgt ggt aaa atc tac aga cgc aat ttt aga cca att ttc ttg cga gaa       1248
Cys Gly Lys Ile Tyr Arg Arg Asn Phe Arg Pro Ile Phe Leu Arg Glu           
                405                 410                 415               

gat ggt gtc act cct ttg cct tct aaa aaa atc tac gat tta gtt ggc       1296
Asp Gly Val Thr Pro Leu Pro Ser Lys Lys Ile Tyr Asp Leu Val Gly           
            420                 425                 430                   

ata tat gca att aaa cta gca ttt ggt tac atg gtg caa cca ttt att       1344
Ile Tyr Ala Ile Lys Leu Ala Phe Gly Tyr Met Val Gln Pro Phe Ile           
        435                 440                 445                       

atc ctt gat ttg aag cca tct tta atg gta tgg ggc tct gtt tat ttc       1392
Ile Leu Asp Leu Lys Pro Ser Leu Met Val Trp Gly Ser Val Tyr Phe           
    450                 455                 460                           

tat gtt cat att att gtt gct ttc tca ttt ttc cta ttc aga gga cca       1440
Tyr Val His Ile Ile Val Ala Phe Ser Phe Phe Leu Phe Arg Gly Pro           
465                 470                 475                 480           

tat gct aaa caa gtt act gaa ttt ttt aaa tcc aaa caa cct aaa gaa       1488
Tyr Ala Lys Gln Val Thr Glu Phe Phe Lys Ser Lys Gln Pro Lys Glu           
                485                 490                 495               

ata ttc att aga aaa caa aag aag ttg gaa aaa gat att tct gca agc       1536
Ile Phe Ile Arg Lys Gln Lys Lys Leu Glu Lys Asp Ile Ser Ala Ser           
            500                 505                 510                   

tct cca aac ttg ggt ggt ata ttg aag gca aag att gaa cat gaa aag       1584
Ser Pro Asn Leu Gly Gly Ile Leu Lys Ala Lys Ile Glu His Glu Lys           
        515                 520                 525                       

gga aag aca gca gaa gaa gaa gaa atg aac tta ggt att cca cca att       1632
Gly Lys Thr Ala Glu Glu Glu Glu Met Asn Leu Gly Ile Pro Pro Ile           
    530                 535                 540                           

gag tta gaa aag tgg gac aat gct aag gaa gat tgg gaa gat ttc tgc       1680
Glu Leu Glu Lys Trp Asp Asn Ala Lys Glu Asp Trp Glu Asp Phe Cys           
545                 550                 555                 560           

aaa gat tac aaa gaa tgg aga aat aaa aat ggt ctt gaa ata gaa gag       1728
Lys Asp Tyr Lys Glu Trp Arg Asn Lys Asn Gly Leu Glu Ile Glu Glu           
                565                 570                 575               

gaa aac ctt tct aaa gct ttt gaa aga ttc aag cag gaa ttt tct aac       1776
Glu Asn Leu Ser Lys Ala Phe Glu Arg Phe Lys Gln Glu Phe Ser Asn           
            580                 585                 590                   

gct gca agt gga tca ggt gaa cgt gtg aga aaa atg agt ttt agt ggt       1824
Ala Ala Ser Gly Ser Gly Glu Arg Val Arg Lys Met Ser Phe Ser Gly           
        595                 600                 605                       

tac tca cca aag cct att tca aaa aag gaa gag tag                       1860
Tyr Ser Pro Lys Pro Ile Ser Lys Lys Glu Glu                               
    610                 615                                               


<210>  15
<211>  619
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  15

Met Tyr Asn Pro Val Asp Ala Val Leu Thr Lys Ile Ile Thr Asn Tyr 
1               5                   10                  15      


Gly Ile Asp Ser Phe Thr Leu Arg Tyr Ala Ile Cys Leu Leu Gly Ser 
            20                  25                  30          


Phe Pro Leu Asn Ala Ile Leu Lys Arg Ile Pro Glu Lys Arg Ile Gly 
        35                  40                  45              


Leu Lys Cys Cys Phe Ile Ile Ser Met Ser Met Phe Tyr Leu Phe Gly 
    50                  55                  60                  


Val Leu Asn Leu Val Ser Gly Phe Arg Thr Leu Phe Ile Ser Thr Met 
65                  70                  75                  80  


Phe Thr Tyr Leu Ile Ser Arg Phe Tyr Arg Ser Lys Phe Met Pro His 
                85                  90                  95      


Leu Asn Phe Met Phe Val Met Gly His Leu Ala Ile Asn His Ile His 
            100                 105                 110         


Ala Gln Phe Leu Asn Glu Gln Thr Gln Thr Thr Val Asp Ile Thr Ser 
        115                 120                 125             


Ser Gln Met Val Leu Ala Met Lys Leu Thr Ser Phe Ala Trp Ser Tyr 
    130                 135                 140                 


Tyr Asp Gly Ser Cys Thr Ser Glu Ser Asp Phe Lys Asp Leu Thr Glu 
145                 150                 155                 160 


His Gln Lys Ser Arg Ala Val Arg Gly His Pro Pro Leu Leu Lys Phe 
                165                 170                 175     


Leu Ala Tyr Ala Phe Phe Tyr Ser Thr Leu Leu Thr Gly Pro Ser Phe 
            180                 185                 190         


Asp Tyr Ala Asp Phe Asp Ser Trp Leu Asn Cys Glu Met Phe Arg Asp 
        195                 200                 205             


Leu Pro Glu Ser Lys Lys Pro Met Arg Arg His His Pro Gly Glu Arg 
    210                 215                 220                 


Arg Gln Ile Pro Lys Asn Gly Lys Leu Ala Leu Trp Lys Val Val Gln 
225                 230                 235                 240 


Gly Leu Ala Trp Met Ile Leu Ser Thr Leu Gly Met Lys His Phe Pro 
                245                 250                 255     


Val Lys Tyr Val Leu Asp Lys Asp Gly Phe Pro Thr Arg Ser Phe Ile 
            260                 265                 270         


Phe Arg Ile His Tyr Leu Phe Leu Leu Gly Phe Ile His Arg Phe Lys 
        275                 280                 285             


Tyr Tyr Ala Ala Trp Thr Ile Ser Glu Gly Ser Cys Ile Leu Cys Gly 
    290                 295                 300                 


Leu Gly Tyr Asn Gly Tyr Asp Ser Lys Thr Gln Lys Ile Arg Trp Asp 
305                 310                 315                 320 


Arg Val Arg Asn Ile Asp Ile Trp Thr Val Glu Thr Ala Gln Asn Thr 
                325                 330                 335     


Arg Glu Met Leu Glu Ala Trp Asn Met Asn Thr Asn Lys Trp Leu Lys 
            340                 345                 350         


Tyr Ser Val Tyr Leu Arg Val Thr Lys Lys Gly Lys Lys Pro Gly Phe 
        355                 360                 365             


Arg Ser Thr Leu Phe Thr Phe Leu Thr Ser Ala Phe Trp His Gly Thr 
    370                 375                 380                 


Arg Pro Gly Tyr Tyr Leu Thr Phe Ala Thr Gly Ala Leu Tyr Gln Thr 
385                 390                 395                 400 


Cys Gly Lys Ile Tyr Arg Arg Asn Phe Arg Pro Ile Phe Leu Arg Glu 
                405                 410                 415     


Asp Gly Val Thr Pro Leu Pro Ser Lys Lys Ile Tyr Asp Leu Val Gly 
            420                 425                 430         


Ile Tyr Ala Ile Lys Leu Ala Phe Gly Tyr Met Val Gln Pro Phe Ile 
        435                 440                 445             


Ile Leu Asp Leu Lys Pro Ser Leu Met Val Trp Gly Ser Val Tyr Phe 
    450                 455                 460                 


Tyr Val His Ile Ile Val Ala Phe Ser Phe Phe Leu Phe Arg Gly Pro 
465                 470                 475                 480 


Tyr Ala Lys Gln Val Thr Glu Phe Phe Lys Ser Lys Gln Pro Lys Glu 
                485                 490                 495     


Ile Phe Ile Arg Lys Gln Lys Lys Leu Glu Lys Asp Ile Ser Ala Ser 
            500                 505                 510         


Ser Pro Asn Leu Gly Gly Ile Leu Lys Ala Lys Ile Glu His Glu Lys 
        515                 520                 525             


Gly Lys Thr Ala Glu Glu Glu Glu Met Asn Leu Gly Ile Pro Pro Ile 
    530                 535                 540                 


Glu Leu Glu Lys Trp Asp Asn Ala Lys Glu Asp Trp Glu Asp Phe Cys 
545                 550                 555                 560 


Lys Asp Tyr Lys Glu Trp Arg Asn Lys Asn Gly Leu Glu Ile Glu Glu 
                565                 570                 575     


Glu Asn Leu Ser Lys Ala Phe Glu Arg Phe Lys Gln Glu Phe Ser Asn 
            580                 585                 590         


Ala Ala Ser Gly Ser Gly Glu Arg Val Arg Lys Met Ser Phe Ser Gly 
        595                 600                 605             


Tyr Ser Pro Lys Pro Ile Ser Lys Lys Glu Glu 
    610                 615                 


<210>  16
<211>  1539
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1539)
<223>  GenBank Accession No. XP_505624; "YALI0F19514p"

<400>  16
atg gcc ttt cca tgg gca gat aag tgg gca gcc gat gcg tct gca tct         48
Met Ala Phe Pro Trp Ala Asp Lys Trp Ala Ala Asp Ala Ser Ala Ser           
1               5                   10                  15                

aca ggg ctg cct ccg gac ctc ctc aag att gca ttc act ctg gtc atg         96
Thr Gly Leu Pro Pro Asp Leu Leu Lys Ile Ala Phe Thr Leu Val Met           
            20                  25                  30                    

tct tat ccg ctg agt tct ctc atg aaa cgg ctg cca gat gac gcc aaa        144
Ser Tyr Pro Leu Ser Ser Leu Met Lys Arg Leu Pro Asp Asp Ala Lys           
        35                  40                  45                        

aac ctc aag atc atc tat atc atc tcc gtg tcc atc ttc tac atg gtg        192
Asn Leu Lys Ile Ile Tyr Ile Ile Ser Val Ser Ile Phe Tyr Met Val           
    50                  55                  60                            

ggt gtc ttc tcc ctc tat ggc gga gct gcc act ctg ctc ttc tcc tca        240
Gly Val Phe Ser Leu Tyr Gly Gly Ala Ala Thr Leu Leu Phe Ser Ser           
65                  70                  75                  80            

atg ggt acc ttc ttc atc acc caa tgg aag agc cct tac atg ccc tgg        288
Met Gly Thr Phe Phe Ile Thr Gln Trp Lys Ser Pro Tyr Met Pro Trp           
                85                  90                  95                

gtc aat ttt ggt ttt gtc atg acc cat ctc ttc gtc aat cac ctg cgt        336
Val Asn Phe Gly Phe Val Met Thr His Leu Phe Val Asn His Leu Arg           
            100                 105                 110                   

tcg cag ttt ttc ccc gaa aca tac gac ccc aat gtc att gac atc acc        384
Ser Gln Phe Phe Pro Glu Thr Tyr Asp Pro Asn Val Ile Asp Ile Thr           
        115                 120                 125                       

gga gca cag atg gtt ctg tgt atg aag cta tcg tct ttt gga tgg aac        432
Gly Ala Gln Met Val Leu Cys Met Lys Leu Ser Ser Phe Gly Trp Asn           
    130                 135                 140                           

gtc tac gat gga tgg cag att gag aag ggt gag cag ctc agc gag ttc        480
Val Tyr Asp Gly Trp Gln Ile Glu Lys Gly Glu Gln Leu Ser Glu Phe           
145                 150                 155                 160           

cag act aaa agg gct gtt ctc aag cac ccc agt ctt atg gac ttc cta        528
Gln Thr Lys Arg Ala Val Leu Lys His Pro Ser Leu Met Asp Phe Leu           
                165                 170                 175               

gct ttt gtg ttc tac ttc cct tcc att ctg aca ggt cct tct tac gac        576
Ala Phe Val Phe Tyr Phe Pro Ser Ile Leu Thr Gly Pro Ser Tyr Asp           
            180                 185                 190                   

tat atg gag ttc cat aac tgg ctc gat ctc agc ctg ttc aag gag ctg        624
Tyr Met Glu Phe His Asn Trp Leu Asp Leu Ser Leu Phe Lys Glu Leu           
        195                 200                 205                       

gag aaa gat aag gac ccc aag cga gct gct cga cga aag cga cac aag        672
Glu Lys Asp Lys Asp Pro Lys Arg Ala Ala Arg Arg Lys Arg His Lys           
    210                 215                 220                           

atc ccc cga tct gga atc gct gct tcc aag aaa ctc gcc gct ggt atc        720
Ile Pro Arg Ser Gly Ile Ala Ala Ser Lys Lys Leu Ala Ala Gly Ile           
225                 230                 235                 240           

ttc tgg atc gtt ctg tgg acc cag gtg gac tct cga atc tcc acc gcc        768
Phe Trp Ile Val Leu Trp Thr Gln Val Asp Ser Arg Ile Ser Thr Ala           
                245                 250                 255               

tac gct tac tca gac gca ttc acc aag gag cac aac atc ttt gga cga        816
Tyr Ala Tyr Ser Asp Ala Phe Thr Lys Glu His Asn Ile Phe Gly Arg           
            260                 265                 270                   

att gtg tac ctc tac atg ctc ggt ttc atg tac cga ctc aag tac tac        864
Ile Val Tyr Leu Tyr Met Leu Gly Phe Met Tyr Arg Leu Lys Tyr Tyr           
        275                 280                 285                       

gga gcc tgg tcc att tcc gag gga gcc tgc atc ttg tct ggc ctc gga        912
Gly Ala Trp Ser Ile Ser Glu Gly Ala Cys Ile Leu Ser Gly Leu Gly           
    290                 295                 300                           

ttc cat ggc gtg gac ccc aaa act ggc aag tac aag tgg gac cgt gtc        960
Phe His Gly Val Asp Pro Lys Thr Gly Lys Tyr Lys Trp Asp Arg Val           
305                 310                 315                 320           

cag aac gtg gac ccg tgg gga ttc gaa act ggt caa aac aca aag gct       1008
Gln Asn Val Asp Pro Trp Gly Phe Glu Thr Gly Gln Asn Thr Lys Ala           
                325                 330                 335               

ctg ctg gag gcc tgg aac cag aac act aac aag tgg cta cga aac tat       1056
Leu Leu Glu Ala Trp Asn Gln Asn Thr Asn Lys Trp Leu Arg Asn Tyr           
            340                 345                 350                   

gtg tac ctc cga gtg gtg ccc aaa ggc caa aag cct gga ttc cga gcc       1104
Val Tyr Leu Arg Val Val Pro Lys Gly Gln Lys Pro Gly Phe Arg Ala           
        355                 360                 365                       

act atc ttc aca ttt gtg gtt tcc gcc ttc tgg cat gga act cga cct       1152
Thr Ile Phe Thr Phe Val Val Ser Ala Phe Trp His Gly Thr Arg Pro           
    370                 375                 380                           

ggc tac tat ctc acc ttt gtg acc gct gcc atg tac cag tct gtt ggt       1200
Gly Tyr Tyr Leu Thr Phe Val Thr Ala Ala Met Tyr Gln Ser Val Gly           
385                 390                 395                 400           

aag ttc ttc cga cga tac ctg cga ccc ttc ttc atg gag tct gat gga       1248
Lys Phe Phe Arg Arg Tyr Leu Arg Pro Phe Phe Met Glu Ser Asp Gly           
                405                 410                 415               

aag act gcc ggt ccc tat aag atc tac tac gac att gtg tgt tgg atc       1296
Lys Thr Ala Gly Pro Tyr Lys Ile Tyr Tyr Asp Ile Val Cys Trp Ile           
            420                 425                 430                   

gtt gtc caa acc gca ttt gga tac gct acc cag tcc ttt atg att cta       1344
Val Val Gln Thr Ala Phe Gly Tyr Ala Thr Gln Ser Phe Met Ile Leu           
        435                 440                 445                       

gac ttc tgg ctg tcg ctc aag tgt tgg aag aac tcc tgg ttc ctg tac       1392
Asp Phe Trp Leu Ser Leu Lys Cys Trp Lys Asn Ser Trp Phe Leu Tyr           
    450                 455                 460                           

cac att gct ctg ggc gcc atc ttt gca att tct agc ccc tac aag gca       1440
His Ile Ala Leu Gly Ala Ile Phe Ala Ile Ser Ser Pro Tyr Lys Ala           
465                 470                 475                 480           

tgg gcg att ccc aag atc aag aaa aag cag gct gga gcc gtc act gac       1488
Trp Ala Ile Pro Lys Ile Lys Lys Lys Gln Ala Gly Ala Val Thr Asp           
                485                 490                 495               

aag aag gac gcc aag gag gag gtg aag aag gac acc atc aag acc aag       1536
Lys Lys Asp Ala Lys Glu Glu Val Lys Lys Asp Thr Ile Lys Thr Lys           
            500                 505                 510                   

taa                                                                   1539


<210>  17
<211>  512
<212>  PRT
<213>  Yarrowia lipolytica

<400>  17

Met Ala Phe Pro Trp Ala Asp Lys Trp Ala Ala Asp Ala Ser Ala Ser 
1               5                   10                  15      


Thr Gly Leu Pro Pro Asp Leu Leu Lys Ile Ala Phe Thr Leu Val Met 
            20                  25                  30          


Ser Tyr Pro Leu Ser Ser Leu Met Lys Arg Leu Pro Asp Asp Ala Lys 
        35                  40                  45              


Asn Leu Lys Ile Ile Tyr Ile Ile Ser Val Ser Ile Phe Tyr Met Val 
    50                  55                  60                  


Gly Val Phe Ser Leu Tyr Gly Gly Ala Ala Thr Leu Leu Phe Ser Ser 
65                  70                  75                  80  


Met Gly Thr Phe Phe Ile Thr Gln Trp Lys Ser Pro Tyr Met Pro Trp 
                85                  90                  95      


Val Asn Phe Gly Phe Val Met Thr His Leu Phe Val Asn His Leu Arg 
            100                 105                 110         


Ser Gln Phe Phe Pro Glu Thr Tyr Asp Pro Asn Val Ile Asp Ile Thr 
        115                 120                 125             


Gly Ala Gln Met Val Leu Cys Met Lys Leu Ser Ser Phe Gly Trp Asn 
    130                 135                 140                 


Val Tyr Asp Gly Trp Gln Ile Glu Lys Gly Glu Gln Leu Ser Glu Phe 
145                 150                 155                 160 


Gln Thr Lys Arg Ala Val Leu Lys His Pro Ser Leu Met Asp Phe Leu 
                165                 170                 175     


Ala Phe Val Phe Tyr Phe Pro Ser Ile Leu Thr Gly Pro Ser Tyr Asp 
            180                 185                 190         


Tyr Met Glu Phe His Asn Trp Leu Asp Leu Ser Leu Phe Lys Glu Leu 
        195                 200                 205             


Glu Lys Asp Lys Asp Pro Lys Arg Ala Ala Arg Arg Lys Arg His Lys 
    210                 215                 220                 


Ile Pro Arg Ser Gly Ile Ala Ala Ser Lys Lys Leu Ala Ala Gly Ile 
225                 230                 235                 240 


Phe Trp Ile Val Leu Trp Thr Gln Val Asp Ser Arg Ile Ser Thr Ala 
                245                 250                 255     


Tyr Ala Tyr Ser Asp Ala Phe Thr Lys Glu His Asn Ile Phe Gly Arg 
            260                 265                 270         


Ile Val Tyr Leu Tyr Met Leu Gly Phe Met Tyr Arg Leu Lys Tyr Tyr 
        275                 280                 285             


Gly Ala Trp Ser Ile Ser Glu Gly Ala Cys Ile Leu Ser Gly Leu Gly 
    290                 295                 300                 


Phe His Gly Val Asp Pro Lys Thr Gly Lys Tyr Lys Trp Asp Arg Val 
305                 310                 315                 320 


Gln Asn Val Asp Pro Trp Gly Phe Glu Thr Gly Gln Asn Thr Lys Ala 
                325                 330                 335     


Leu Leu Glu Ala Trp Asn Gln Asn Thr Asn Lys Trp Leu Arg Asn Tyr 
            340                 345                 350         


Val Tyr Leu Arg Val Val Pro Lys Gly Gln Lys Pro Gly Phe Arg Ala 
        355                 360                 365             


Thr Ile Phe Thr Phe Val Val Ser Ala Phe Trp His Gly Thr Arg Pro 
    370                 375                 380                 


Gly Tyr Tyr Leu Thr Phe Val Thr Ala Ala Met Tyr Gln Ser Val Gly 
385                 390                 395                 400 


Lys Phe Phe Arg Arg Tyr Leu Arg Pro Phe Phe Met Glu Ser Asp Gly 
                405                 410                 415     


Lys Thr Ala Gly Pro Tyr Lys Ile Tyr Tyr Asp Ile Val Cys Trp Ile 
            420                 425                 430         


Val Val Gln Thr Ala Phe Gly Tyr Ala Thr Gln Ser Phe Met Ile Leu 
        435                 440                 445             


Asp Phe Trp Leu Ser Leu Lys Cys Trp Lys Asn Ser Trp Phe Leu Tyr 
    450                 455                 460                 


His Ile Ala Leu Gly Ala Ile Phe Ala Ile Ser Ser Pro Tyr Lys Ala 
465                 470                 475                 480 


Trp Ala Ile Pro Lys Ile Lys Lys Lys Gln Ala Gly Ala Val Thr Asp 
                485                 490                 495     


Lys Lys Asp Ala Lys Glu Glu Val Lys Lys Asp Thr Ile Lys Thr Lys 
            500                 505                 510         


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  membrane bound O-acyltransferase motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = Val [V] or Ile [I]

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  18

Met Xaa Leu Xaa Xaa Lys Leu 
1               5           


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  membrane bound O-acyltransferase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  19

Arg Xaa Lys Tyr Tyr Xaa Xaa Trp 
1               5               


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  membrane bound O-acyltransferase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  20

Ser Ala Xaa Trp His Gly 
1               5       


<210>  21
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif


<220>
<221>  misc_feature
<222>  (2)..(12)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (15)..(16)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa = Thr [T] or Val [V]

<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  GENES ENCODING A NOVEL TYPE OF LYSOPHOPHATIDYLCHOLINE 
       ACYLTRANSFERASES AND THEIR USE TO INCREASE TRIACYLGLYCEROL 
       PRODUCTION AND/OR MODIFY FATTY ACID COMPOSITION
<310>  U.S. Patent Publication No. 2008-0145867-A1
<311>  2007-06-15
<312>  2008-06-19
<313>  (1)..(15)

<400>  21

Glu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Asn Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Trp 
            20  


<210>  22
<211>  1870
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (3)..(1862)
<223>  synthetic Ale1 (codon-optimized for Yarrowia lipolytica)

<400>  22
ac atg tac aac ccc gtg gac gca gtg ttg act aag att att aca aac          47
   Met Tyr Asn Pro Val Asp Ala Val Leu Thr Lys Ile Ile Thr Asn            
   1               5                   10                  15             

tac gga att gat tct ttt acc ctg cga tat gcc att tgt ctg ttg gga         95
Tyr Gly Ile Asp Ser Phe Thr Leu Arg Tyr Ala Ile Cys Leu Leu Gly           
                20                  25                  30                

tct ttt cct ctt aac gct att ctg aag cgg att cct gaa aag cga atc        143
Ser Phe Pro Leu Asn Ala Ile Leu Lys Arg Ile Pro Glu Lys Arg Ile           
            35                  40                  45                    

ggc ctg aag tgt tgt ttt atc att tct atg tcc atg ttt tat ctc ttc        191
Gly Leu Lys Cys Cys Phe Ile Ile Ser Met Ser Met Phe Tyr Leu Phe           
        50                  55                  60                        

ggc gtt ctg aat ctc gtg agc gga ttt cga acc ctc ttc att tcc aca        239
Gly Val Leu Asn Leu Val Ser Gly Phe Arg Thr Leu Phe Ile Ser Thr           
    65                  70                  75                            

atg ttc aca tac ctt atc tct cgg ttc tac cga tcc aag ttt atg ccc        287
Met Phe Thr Tyr Leu Ile Ser Arg Phe Tyr Arg Ser Lys Phe Met Pro           
80                  85                  90                  95            

cat ctc aac ttc atg ttc gtc atg ggc cac ttg gct atc aac cac att        335
His Leu Asn Phe Met Phe Val Met Gly His Leu Ala Ile Asn His Ile           
                100                 105                 110               

cat gct cag ttc ctg aac gaa caa act caa acg acc gtc gat att aca        383
His Ala Gln Phe Leu Asn Glu Gln Thr Gln Thr Thr Val Asp Ile Thr           
            115                 120                 125                   

tcc tcg cag atg gtc ctg gct atg aag ctg aca agc ttt gcc tgg tct        431
Ser Ser Gln Met Val Leu Ala Met Lys Leu Thr Ser Phe Ala Trp Ser           
        130                 135                 140                       

tac tat gac ggt tcg tgt acg agc gag tcc gac ttc aag gac ctt acc        479
Tyr Tyr Asp Gly Ser Cys Thr Ser Glu Ser Asp Phe Lys Asp Leu Thr           
    145                 150                 155                           

gaa cac cag aag tcc cga gcc gtc cga ggc cat cct ccc ctt ctg aaa        527
Glu His Gln Lys Ser Arg Ala Val Arg Gly His Pro Pro Leu Leu Lys           
160                 165                 170                 175           

ttt ttg gct tac gcc ttt ttc tac tct acc ctt ctc acc ggt ccc tcc        575
Phe Leu Ala Tyr Ala Phe Phe Tyr Ser Thr Leu Leu Thr Gly Pro Ser           
                180                 185                 190               

ttc gat tac gct gat ttc gac tct tgg ctg aac tgc gaa atg ttc cgg        623
Phe Asp Tyr Ala Asp Phe Asp Ser Trp Leu Asn Cys Glu Met Phe Arg           
            195                 200                 205                   

gac ctt ccc gag tcc aag aaa ccc atg cga aga cat cat cct ggt gag        671
Asp Leu Pro Glu Ser Lys Lys Pro Met Arg Arg His His Pro Gly Glu           
        210                 215                 220                       

cgg cgt cag att ccc aag aac ggc aag ctc gcc ctg tgg aag gtt gtc        719
Arg Arg Gln Ile Pro Lys Asn Gly Lys Leu Ala Leu Trp Lys Val Val           
    225                 230                 235                           

cag ggc ctc gcc tgg atg att ctg agc acg ttg ggt atg aag cac ttc        767
Gln Gly Leu Ala Trp Met Ile Leu Ser Thr Leu Gly Met Lys His Phe           
240                 245                 250                 255           

ccc gtg aag tac gtg ctg gac aag gac gga ttt cct acc cgt tcc ttt        815
Pro Val Lys Tyr Val Leu Asp Lys Asp Gly Phe Pro Thr Arg Ser Phe           
                260                 265                 270               

atc ttc cgt att cat tat ctg ttt ctg ctg gga ttc atc cac cga ttt        863
Ile Phe Arg Ile His Tyr Leu Phe Leu Leu Gly Phe Ile His Arg Phe           
            275                 280                 285                   

aag tat tac gct gcg tgg acg att agc gaa ggt tcg tgc att ctc tgt        911
Lys Tyr Tyr Ala Ala Trp Thr Ile Ser Glu Gly Ser Cys Ile Leu Cys           
        290                 295                 300                       

ggt ctt ggt tat aat gga tac gat tct aag acc cag aag atc cgg tgg        959
Gly Leu Gly Tyr Asn Gly Tyr Asp Ser Lys Thr Gln Lys Ile Arg Trp           
    305                 310                 315                           

gat cga gtg cgg aat att gat att tgg aca gtg gag act gca caa aac       1007
Asp Arg Val Arg Asn Ile Asp Ile Trp Thr Val Glu Thr Ala Gln Asn           
320                 325                 330                 335           

acc cga gag atg ctg gaa gcg tgg aac atg aat act aac aaa tgg ctg       1055
Thr Arg Glu Met Leu Glu Ala Trp Asn Met Asn Thr Asn Lys Trp Leu           
                340                 345                 350               

aag tat agc gtg tat ctt aga gtg act aag aag ggt aag aag cca ggt       1103
Lys Tyr Ser Val Tyr Leu Arg Val Thr Lys Lys Gly Lys Lys Pro Gly           
            355                 360                 365                   

ttt cga tct acc ctg ttt acc ttc ctg acc tcc gcc ttt tgg cac ggt       1151
Phe Arg Ser Thr Leu Phe Thr Phe Leu Thr Ser Ala Phe Trp His Gly           
        370                 375                 380                       

acc cgt cct gga tac tac ctt acc ttc gca act ggt gcc ctg tac caa       1199
Thr Arg Pro Gly Tyr Tyr Leu Thr Phe Ala Thr Gly Ala Leu Tyr Gln           
    385                 390                 395                           

acc tgt gga aag atc tat aga cga aac ttt cgt ccc atc ttt ctg aga       1247
Thr Cys Gly Lys Ile Tyr Arg Arg Asn Phe Arg Pro Ile Phe Leu Arg           
400                 405                 410                 415           

gaa gat ggc gtg aca cct ctc ccg tcc aag aag att tac gac ctg gtc       1295
Glu Asp Gly Val Thr Pro Leu Pro Ser Lys Lys Ile Tyr Asp Leu Val           
                420                 425                 430               

ggc att tac gct att aag ctg gcc ttt ggt tac atg gtt caa ccc ttc       1343
Gly Ile Tyr Ala Ile Lys Leu Ala Phe Gly Tyr Met Val Gln Pro Phe           
            435                 440                 445                   

att atc ctt gac ctg aag ccc tct ctt atg gtt tgg gga tcc gtg tat       1391
Ile Ile Leu Asp Leu Lys Pro Ser Leu Met Val Trp Gly Ser Val Tyr           
        450                 455                 460                       

ttc tac gtg cat att att gtg gcc ttc tcg ttc ttt ctg ttc cga gga       1439
Phe Tyr Val His Ile Ile Val Ala Phe Ser Phe Phe Leu Phe Arg Gly           
    465                 470                 475                           

cca tac gct aag cag gtt act gaa ttt ttc aaa agc aag caa ccg aag       1487
Pro Tyr Ala Lys Gln Val Thr Glu Phe Phe Lys Ser Lys Gln Pro Lys           
480                 485                 490                 495           

gag atc ttc atc cga aag cag aag aag ttg gaa aaa gac atc tct gcc       1535
Glu Ile Phe Ile Arg Lys Gln Lys Lys Leu Glu Lys Asp Ile Ser Ala           
                500                 505                 510               

tct tcc ccc aac ctc gga ggt att ctt aag gca aaa atc gaa cat gag       1583
Ser Ser Pro Asn Leu Gly Gly Ile Leu Lys Ala Lys Ile Glu His Glu           
            515                 520                 525                   

aag gga aag acg gca gag gag gaa gag atg aac ttg ggc att cca ccc       1631
Lys Gly Lys Thr Ala Glu Glu Glu Glu Met Asn Leu Gly Ile Pro Pro           
        530                 535                 540                       

atc gaa ctg gag aag tgg gac aac gcc aag gag gac tgg gag gat ttc       1679
Ile Glu Leu Glu Lys Trp Asp Asn Ala Lys Glu Asp Trp Glu Asp Phe           
    545                 550                 555                           

tgc aag gac tac aag gag tgg cgg aac aag aac gga ctg gaa att gaa       1727
Cys Lys Asp Tyr Lys Glu Trp Arg Asn Lys Asn Gly Leu Glu Ile Glu           
560                 565                 570                 575           

gag gag aac ctg tcc aag gcc ttc gag cga ttt aag cag gaa ttt tcc       1775
Glu Glu Asn Leu Ser Lys Ala Phe Glu Arg Phe Lys Gln Glu Phe Ser           
                580                 585                 590               

aac gct gcg tcg ggc tct ggt gaa cgg gtt cgg aaa atg tcc ttc tcc       1823
Asn Ala Ala Ser Gly Ser Gly Glu Arg Val Arg Lys Met Ser Phe Ser           
            595                 600                 605                   

gga tat tct cct aaa ccc atc tcg aag aaa gaa gaa tag gcggccgc          1870
Gly Tyr Ser Pro Lys Pro Ile Ser Lys Lys Glu Glu                           
        610                 615                                           


<210>  23
<211>  619
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  23

Met Tyr Asn Pro Val Asp Ala Val Leu Thr Lys Ile Ile Thr Asn Tyr 
1               5                   10                  15      


Gly Ile Asp Ser Phe Thr Leu Arg Tyr Ala Ile Cys Leu Leu Gly Ser 
            20                  25                  30          


Phe Pro Leu Asn Ala Ile Leu Lys Arg Ile Pro Glu Lys Arg Ile Gly 
        35                  40                  45              


Leu Lys Cys Cys Phe Ile Ile Ser Met Ser Met Phe Tyr Leu Phe Gly 
    50                  55                  60                  


Val Leu Asn Leu Val Ser Gly Phe Arg Thr Leu Phe Ile Ser Thr Met 
65                  70                  75                  80  


Phe Thr Tyr Leu Ile Ser Arg Phe Tyr Arg Ser Lys Phe Met Pro His 
                85                  90                  95      


Leu Asn Phe Met Phe Val Met Gly His Leu Ala Ile Asn His Ile His 
            100                 105                 110         


Ala Gln Phe Leu Asn Glu Gln Thr Gln Thr Thr Val Asp Ile Thr Ser 
        115                 120                 125             


Ser Gln Met Val Leu Ala Met Lys Leu Thr Ser Phe Ala Trp Ser Tyr 
    130                 135                 140                 


Tyr Asp Gly Ser Cys Thr Ser Glu Ser Asp Phe Lys Asp Leu Thr Glu 
145                 150                 155                 160 


His Gln Lys Ser Arg Ala Val Arg Gly His Pro Pro Leu Leu Lys Phe 
                165                 170                 175     


Leu Ala Tyr Ala Phe Phe Tyr Ser Thr Leu Leu Thr Gly Pro Ser Phe 
            180                 185                 190         


Asp Tyr Ala Asp Phe Asp Ser Trp Leu Asn Cys Glu Met Phe Arg Asp 
        195                 200                 205             


Leu Pro Glu Ser Lys Lys Pro Met Arg Arg His His Pro Gly Glu Arg 
    210                 215                 220                 


Arg Gln Ile Pro Lys Asn Gly Lys Leu Ala Leu Trp Lys Val Val Gln 
225                 230                 235                 240 


Gly Leu Ala Trp Met Ile Leu Ser Thr Leu Gly Met Lys His Phe Pro 
                245                 250                 255     


Val Lys Tyr Val Leu Asp Lys Asp Gly Phe Pro Thr Arg Ser Phe Ile 
            260                 265                 270         


Phe Arg Ile His Tyr Leu Phe Leu Leu Gly Phe Ile His Arg Phe Lys 
        275                 280                 285             


Tyr Tyr Ala Ala Trp Thr Ile Ser Glu Gly Ser Cys Ile Leu Cys Gly 
    290                 295                 300                 


Leu Gly Tyr Asn Gly Tyr Asp Ser Lys Thr Gln Lys Ile Arg Trp Asp 
305                 310                 315                 320 


Arg Val Arg Asn Ile Asp Ile Trp Thr Val Glu Thr Ala Gln Asn Thr 
                325                 330                 335     


Arg Glu Met Leu Glu Ala Trp Asn Met Asn Thr Asn Lys Trp Leu Lys 
            340                 345                 350         


Tyr Ser Val Tyr Leu Arg Val Thr Lys Lys Gly Lys Lys Pro Gly Phe 
        355                 360                 365             


Arg Ser Thr Leu Phe Thr Phe Leu Thr Ser Ala Phe Trp His Gly Thr 
    370                 375                 380                 


Arg Pro Gly Tyr Tyr Leu Thr Phe Ala Thr Gly Ala Leu Tyr Gln Thr 
385                 390                 395                 400 


Cys Gly Lys Ile Tyr Arg Arg Asn Phe Arg Pro Ile Phe Leu Arg Glu 
                405                 410                 415     


Asp Gly Val Thr Pro Leu Pro Ser Lys Lys Ile Tyr Asp Leu Val Gly 
            420                 425                 430         


Ile Tyr Ala Ile Lys Leu Ala Phe Gly Tyr Met Val Gln Pro Phe Ile 
        435                 440                 445             


Ile Leu Asp Leu Lys Pro Ser Leu Met Val Trp Gly Ser Val Tyr Phe 
    450                 455                 460                 


Tyr Val His Ile Ile Val Ala Phe Ser Phe Phe Leu Phe Arg Gly Pro 
465                 470                 475                 480 


Tyr Ala Lys Gln Val Thr Glu Phe Phe Lys Ser Lys Gln Pro Lys Glu 
                485                 490                 495     


Ile Phe Ile Arg Lys Gln Lys Lys Leu Glu Lys Asp Ile Ser Ala Ser 
            500                 505                 510         


Ser Pro Asn Leu Gly Gly Ile Leu Lys Ala Lys Ile Glu His Glu Lys 
        515                 520                 525             


Gly Lys Thr Ala Glu Glu Glu Glu Met Asn Leu Gly Ile Pro Pro Ile 
    530                 535                 540                 


Glu Leu Glu Lys Trp Asp Asn Ala Lys Glu Asp Trp Glu Asp Phe Cys 
545                 550                 555                 560 


Lys Asp Tyr Lys Glu Trp Arg Asn Lys Asn Gly Leu Glu Ile Glu Glu 
                565                 570                 575     


Glu Asn Leu Ser Lys Ala Phe Glu Arg Phe Lys Gln Glu Phe Ser Asn 
            580                 585                 590         


Ala Ala Ser Gly Ser Gly Glu Arg Val Arg Lys Met Ser Phe Ser Gly 
        595                 600                 605             


Tyr Ser Pro Lys Pro Ile Ser Lys Lys Glu Glu 
    610                 615                 


<210>  24
<211>  849
<212>  DNA
<213>  Caenorhabditis elegans


<220>
<221>  CDS
<222>  (1)..(849)
<223>  GenBank Accession No. CAA98276; "clone T06E8.1"

<300>
<302>  METHOD FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS
<310>  US 2006-0168687-A1
<311>  2004-01-29
<312>  2006-07-27
<313>  (1)..(849)

<300>
<302>  METHOD FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS
<310>  WO 2004/076617
<311>  2004-01-29
<312>  2004-09-10
<313>  (1)..(849)

<400>  24
atg gag aac ttc tgg tcg atc gtc gtg ttt ttt cta ctc tca att ctc         48
Met Glu Asn Phe Trp Ser Ile Val Val Phe Phe Leu Leu Ser Ile Leu           
1               5                   10                  15                

ttc att tta tat aac ata tcg aca gta tgc cac tac tat atg cgg att         96
Phe Ile Leu Tyr Asn Ile Ser Thr Val Cys His Tyr Tyr Met Arg Ile           
            20                  25                  30                    

tcg ttt tat tac ttc aca att tta ttg cat gga atg gaa gtt tgt gtt        144
Ser Phe Tyr Tyr Phe Thr Ile Leu Leu His Gly Met Glu Val Cys Val           
        35                  40                  45                        

aca atg atc cct tct tgg cta aat ggg aag ggt gct gat tac gtg ttt        192
Thr Met Ile Pro Ser Trp Leu Asn Gly Lys Gly Ala Asp Tyr Val Phe           
    50                  55                  60                            

cac tcg ttt ttc tat tgg tgt aaa tgg act ggt gtt cat aca aca gtc        240
His Ser Phe Phe Tyr Trp Cys Lys Trp Thr Gly Val His Thr Thr Val           
65                  70                  75                  80            

tat gga tat gaa aaa aca caa gtt gaa ggt ccg gct gta gtt att tgt        288
Tyr Gly Tyr Glu Lys Thr Gln Val Glu Gly Pro Ala Val Val Ile Cys           
                85                  90                  95                

aat cat cag agt tct ctc gac att cta tcg atg gca tca atc tgg ccg        336
Asn His Gln Ser Ser Leu Asp Ile Leu Ser Met Ala Ser Ile Trp Pro           
            100                 105                 110                   

aag aat tgt gtt gta atg atg aaa cga att ctt gcc tat gtt cca ttc        384
Lys Asn Cys Val Val Met Met Lys Arg Ile Leu Ala Tyr Val Pro Phe           
        115                 120                 125                       

ttc aat ctc gga gcc tac ttt tcc aac aca atc ttc atc gat cga tat        432
Phe Asn Leu Gly Ala Tyr Phe Ser Asn Thr Ile Phe Ile Asp Arg Tyr           
    130                 135                 140                           

aac cgt gaa cgt gcg atg gct tca gtt gat tat tgt gca tct gaa atg        480
Asn Arg Glu Arg Ala Met Ala Ser Val Asp Tyr Cys Ala Ser Glu Met           
145                 150                 155                 160           

aag aac aga aat ctt aaa ctt tgg gta ttt ccg gaa gga aca aga aat        528
Lys Asn Arg Asn Leu Lys Leu Trp Val Phe Pro Glu Gly Thr Arg Asn           
                165                 170                 175               

cgt gaa gga ggg ttc att cca ttc aag aaa gga gca ttc aat att gca        576
Arg Glu Gly Gly Phe Ile Pro Phe Lys Lys Gly Ala Phe Asn Ile Ala           
            180                 185                 190                   

gtt cgt gcg cag att ccc att att cca gtt gta ttc tca gac tat cgg        624
Val Arg Ala Gln Ile Pro Ile Ile Pro Val Val Phe Ser Asp Tyr Arg           
        195                 200                 205                       

gat ttc tac tca aag cca ggc cga tat ttc aag aat gat gga gaa gtt        672
Asp Phe Tyr Ser Lys Pro Gly Arg Tyr Phe Lys Asn Asp Gly Glu Val           
    210                 215                 220                           

gtt att cga gtt ctg gat gcg att cca aca aaa ggg ctc act ctt gat        720
Val Ile Arg Val Leu Asp Ala Ile Pro Thr Lys Gly Leu Thr Leu Asp           
225                 230                 235                 240           

gac gtc agc gag ttg tct gat atg tgt cgg gac gtt atg ttg gca gcc        768
Asp Val Ser Glu Leu Ser Asp Met Cys Arg Asp Val Met Leu Ala Ala           
                245                 250                 255               

tat aag gaa gtt act cta gaa gct cag caa cga aat gcg aca cgg cgt        816
Tyr Lys Glu Val Thr Leu Glu Ala Gln Gln Arg Asn Ala Thr Arg Arg           
            260                 265                 270                   

gga gaa aca aaa gac ggg aag aaa tct gag taa                            849
Gly Glu Thr Lys Asp Gly Lys Lys Ser Glu                                   
        275                 280                                           


<210>  25
<211>  282
<212>  PRT
<213>  Caenorhabditis elegans

<400>  25

Met Glu Asn Phe Trp Ser Ile Val Val Phe Phe Leu Leu Ser Ile Leu 
1               5                   10                  15      


Phe Ile Leu Tyr Asn Ile Ser Thr Val Cys His Tyr Tyr Met Arg Ile 
            20                  25                  30          


Ser Phe Tyr Tyr Phe Thr Ile Leu Leu His Gly Met Glu Val Cys Val 
        35                  40                  45              


Thr Met Ile Pro Ser Trp Leu Asn Gly Lys Gly Ala Asp Tyr Val Phe 
    50                  55                  60                  


His Ser Phe Phe Tyr Trp Cys Lys Trp Thr Gly Val His Thr Thr Val 
65                  70                  75                  80  


Tyr Gly Tyr Glu Lys Thr Gln Val Glu Gly Pro Ala Val Val Ile Cys 
                85                  90                  95      


Asn His Gln Ser Ser Leu Asp Ile Leu Ser Met Ala Ser Ile Trp Pro 
            100                 105                 110         


Lys Asn Cys Val Val Met Met Lys Arg Ile Leu Ala Tyr Val Pro Phe 
        115                 120                 125             


Phe Asn Leu Gly Ala Tyr Phe Ser Asn Thr Ile Phe Ile Asp Arg Tyr 
    130                 135                 140                 


Asn Arg Glu Arg Ala Met Ala Ser Val Asp Tyr Cys Ala Ser Glu Met 
145                 150                 155                 160 


Lys Asn Arg Asn Leu Lys Leu Trp Val Phe Pro Glu Gly Thr Arg Asn 
                165                 170                 175     


Arg Glu Gly Gly Phe Ile Pro Phe Lys Lys Gly Ala Phe Asn Ile Ala 
            180                 185                 190         


Val Arg Ala Gln Ile Pro Ile Ile Pro Val Val Phe Ser Asp Tyr Arg 
        195                 200                 205             


Asp Phe Tyr Ser Lys Pro Gly Arg Tyr Phe Lys Asn Asp Gly Glu Val 
    210                 215                 220                 


Val Ile Arg Val Leu Asp Ala Ile Pro Thr Lys Gly Leu Thr Leu Asp 
225                 230                 235                 240 


Asp Val Ser Glu Leu Ser Asp Met Cys Arg Asp Val Met Leu Ala Ala 
                245                 250                 255     


Tyr Lys Glu Val Thr Leu Glu Ala Gln Gln Arg Asn Ala Thr Arg Arg 
            260                 265                 270         


Gly Glu Thr Lys Asp Gly Lys Lys Ser Glu 
        275                 280         


<210>  26
<211>  859
<212>  DNA
<213>  Caenorhabditis elegans


<220>
<221>  CDS
<222>  (3)..(851)
<223>  synthetic LPCAT (codon-optimized for Yarrowia lipolytica)

<400>  26
cc atg gag aac ttc tgg tcc atc gtc gtg ttc ttt ctg ctc tcc att          47
   Met Glu Asn Phe Trp Ser Ile Val Val Phe Phe Leu Leu Ser Ile            
   1               5                   10                  15             

ctg ttc atc ctc tac aac att tcg aca gtc tgc cac tac tac atg cga         95
Leu Phe Ile Leu Tyr Asn Ile Ser Thr Val Cys His Tyr Tyr Met Arg           
                20                  25                  30                

atc tcc ttc tac tac ttt acc atc ctg ctt cac ggc atg gag gtg tgc        143
Ile Ser Phe Tyr Tyr Phe Thr Ile Leu Leu His Gly Met Glu Val Cys           
            35                  40                  45                    

gtt acc atg att ccc tct tgg ctc aac ggc aag ggt gcc gac tac gtg        191
Val Thr Met Ile Pro Ser Trp Leu Asn Gly Lys Gly Ala Asp Tyr Val           
        50                  55                  60                        

ttt cac tcg ttc ttc tac tgg tgc aag tgg act gga gtc cac acc act        239
Phe His Ser Phe Phe Tyr Trp Cys Lys Trp Thr Gly Val His Thr Thr           
    65                  70                  75                            

gtg tat ggc tac gag aag acc cag gtc gaa ggt cct gcc gtg gtc atc        287
Val Tyr Gly Tyr Glu Lys Thr Gln Val Glu Gly Pro Ala Val Val Ile           
80                  85                  90                  95            

tgc aac cat cag tcc tcg ctc gac att ctg tct atg gct tcc atc tgg        335
Cys Asn His Gln Ser Ser Leu Asp Ile Leu Ser Met Ala Ser Ile Trp           
                100                 105                 110               

ccc aag aac tgt gtt gtc atg atg aag cgg att ctt gcc tac gtt ccc        383
Pro Lys Asn Cys Val Val Met Met Lys Arg Ile Leu Ala Tyr Val Pro           
            115                 120                 125                   

ttc ttc aac ctg gga gcc tac ttt tcc aac acc atc ttc atc gac cga        431
Phe Phe Asn Leu Gly Ala Tyr Phe Ser Asn Thr Ile Phe Ile Asp Arg           
        130                 135                 140                       

tac aac cga gag cga gct atg gct tct gtc gac tac tgt gcc tcc gag        479
Tyr Asn Arg Glu Arg Ala Met Ala Ser Val Asp Tyr Cys Ala Ser Glu           
    145                 150                 155                           

atg aag aac cga aac ctg aag ctc tgg gtg ttt ccc gaa ggc act cgg        527
Met Lys Asn Arg Asn Leu Lys Leu Trp Val Phe Pro Glu Gly Thr Arg           
160                 165                 170                 175           

aat cga gag ggt gga ttc att ccc ttc aag aaa ggt gcc ttc aac atc        575
Asn Arg Glu Gly Gly Phe Ile Pro Phe Lys Lys Gly Ala Phe Asn Ile           
                180                 185                 190               

gct gtt cga gcc cag att ccc atc att cct gtc gtg ttc tct gac tat        623
Ala Val Arg Ala Gln Ile Pro Ile Ile Pro Val Val Phe Ser Asp Tyr           
            195                 200                 205                   

cga gac ttc tac tcc aag cct ggc cga tac ttc aag aac gat gga gag        671
Arg Asp Phe Tyr Ser Lys Pro Gly Arg Tyr Phe Lys Asn Asp Gly Glu           
        210                 215                 220                       

gtc gtg atc cga gtc ctg gat gcc att ccc acc aag ggt ctg acc ctc        719
Val Val Ile Arg Val Leu Asp Ala Ile Pro Thr Lys Gly Leu Thr Leu           
    225                 230                 235                           

gat gac gtc tct gag ctt tcg gac atg tgt cga gac gtc atg ctg gct        767
Asp Asp Val Ser Glu Leu Ser Asp Met Cys Arg Asp Val Met Leu Ala           
240                 245                 250                 255           

gcc tac aag gaa gtt acc ctc gag gct cag caa cga aac gcc act cga        815
Ala Tyr Lys Glu Val Thr Leu Glu Ala Gln Gln Arg Asn Ala Thr Arg           
                260                 265                 270               

aga gga gag acc aag gac ggc aag aaa tcc gag taa gcggccgc               859
Arg Gly Glu Thr Lys Asp Gly Lys Lys Ser Glu                               
            275                 280                                       


<210>  27
<211>  282
<212>  PRT
<213>  Caenorhabditis elegans

<400>  27

Met Glu Asn Phe Trp Ser Ile Val Val Phe Phe Leu Leu Ser Ile Leu 
1               5                   10                  15      


Phe Ile Leu Tyr Asn Ile Ser Thr Val Cys His Tyr Tyr Met Arg Ile 
            20                  25                  30          


Ser Phe Tyr Tyr Phe Thr Ile Leu Leu His Gly Met Glu Val Cys Val 
        35                  40                  45              


Thr Met Ile Pro Ser Trp Leu Asn Gly Lys Gly Ala Asp Tyr Val Phe 
    50                  55                  60                  


His Ser Phe Phe Tyr Trp Cys Lys Trp Thr Gly Val His Thr Thr Val 
65                  70                  75                  80  


Tyr Gly Tyr Glu Lys Thr Gln Val Glu Gly Pro Ala Val Val Ile Cys 
                85                  90                  95      


Asn His Gln Ser Ser Leu Asp Ile Leu Ser Met Ala Ser Ile Trp Pro 
            100                 105                 110         


Lys Asn Cys Val Val Met Met Lys Arg Ile Leu Ala Tyr Val Pro Phe 
        115                 120                 125             


Phe Asn Leu Gly Ala Tyr Phe Ser Asn Thr Ile Phe Ile Asp Arg Tyr 
    130                 135                 140                 


Asn Arg Glu Arg Ala Met Ala Ser Val Asp Tyr Cys Ala Ser Glu Met 
145                 150                 155                 160 


Lys Asn Arg Asn Leu Lys Leu Trp Val Phe Pro Glu Gly Thr Arg Asn 
                165                 170                 175     


Arg Glu Gly Gly Phe Ile Pro Phe Lys Lys Gly Ala Phe Asn Ile Ala 
            180                 185                 190         


Val Arg Ala Gln Ile Pro Ile Ile Pro Val Val Phe Ser Asp Tyr Arg 
        195                 200                 205             


Asp Phe Tyr Ser Lys Pro Gly Arg Tyr Phe Lys Asn Asp Gly Glu Val 
    210                 215                 220                 


Val Ile Arg Val Leu Asp Ala Ile Pro Thr Lys Gly Leu Thr Leu Asp 
225                 230                 235                 240 


Asp Val Ser Glu Leu Ser Asp Met Cys Arg Asp Val Met Leu Ala Ala 
                245                 250                 255     


Tyr Lys Glu Val Thr Leu Glu Ala Gln Gln Arg Asn Ala Thr Arg Arg 
            260                 265                 270         


Gly Glu Thr Lys Asp Gly Lys Lys Ser Glu 
        275                 280         


<210>  28
<211>  945
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(945)
<223>  LPAAT1

<300>
<302>  High eicosapentaenoic acid producing strains of Yarrowia 
       lipolytica
<310>  US-2006-0115881-A1
<311>  2005-11-02
<312>  2006-06-01
<313>  (1)..(945)

<300>
<302>  High eicosapentaenoic acid producing strains of Yarrowia 
       lipolytica
<310>  WO 2006/052870
<311>  2005-11-03
<312>  2006-05-18
<313>  (1)..(945)

<400>  28
atg tcc ata ggt tct tcc aat cct gtc ctg ctg gca gcg atc ccc ttc         48
Met Ser Ile Gly Ser Ser Asn Pro Val Leu Leu Ala Ala Ile Pro Phe           
1               5                   10                  15                

gtc tac ctc ttc gtc ctc cct cgt gtc ctc gcc ttc ctc cct caa aag         96
Val Tyr Leu Phe Val Leu Pro Arg Val Leu Ala Phe Leu Pro Gln Lys           
            20                  25                  30                    

gcc cag ttc ctc gca aaa tgc atc gtg gtc ttg atc gcc acc ctt atc        144
Ala Gln Phe Leu Ala Lys Cys Ile Val Val Leu Ile Ala Thr Leu Ile           
        35                  40                  45                        

atg tcc gtc gca ggc tgc ttc att tcc atc gtc tgt gcg ctc ctc gat        192
Met Ser Val Ala Gly Cys Phe Ile Ser Ile Val Cys Ala Leu Leu Asp           
    50                  55                  60                            

aaa cgc tat gtg atc aac tac gtc gtc tca aga ctc ttc tca ttc ctc        240
Lys Arg Tyr Val Ile Asn Tyr Val Val Ser Arg Leu Phe Ser Phe Leu           
65                  70                  75                  80            

gct gca aga ccc tgc ggt gtc acc tac aag atc gtc ggc gag gaa cat        288
Ala Ala Arg Pro Cys Gly Val Thr Tyr Lys Ile Val Gly Glu Glu His           
                85                  90                  95                

ctg gac aag tac ccc gcc att gtc gtc tgc aac cac cag agc tcc atg        336
Leu Asp Lys Tyr Pro Ala Ile Val Val Cys Asn His Gln Ser Ser Met           
            100                 105                 110                   

gac atg atg gtc ctg gga cgc gtc ttc cca aag cac tgt gtc gtc atg        384
Asp Met Met Val Leu Gly Arg Val Phe Pro Lys His Cys Val Val Met           
        115                 120                 125                       

gca aag aag gaa ctt ctt tac ttt ccg ttc ctg ggc atg ttt atg aag        432
Ala Lys Lys Glu Leu Leu Tyr Phe Pro Phe Leu Gly Met Phe Met Lys           
    130                 135                 140                           

ctg agt aac gcc atc ttc att gac cgc aag aac cac aag aag gcg atc        480
Leu Ser Asn Ala Ile Phe Ile Asp Arg Lys Asn His Lys Lys Ala Ile           
145                 150                 155                 160           

gag tcc acc acc caa gct gtc gcc gac atg aag aag cac aac tct gga        528
Glu Ser Thr Thr Gln Ala Val Ala Asp Met Lys Lys His Asn Ser Gly           
                165                 170                 175               

atc tgg att ttc ccc gaa gga aca cgt tcc cgc ttg gac aag gcc gat        576
Ile Trp Ile Phe Pro Glu Gly Thr Arg Ser Arg Leu Asp Lys Ala Asp           
            180                 185                 190                   

ctc ttg ccc ttc aag aag gga gcc ttc cac ctc gcc att caa gcc caa        624
Leu Leu Pro Phe Lys Lys Gly Ala Phe His Leu Ala Ile Gln Ala Gln           
        195                 200                 205                       

ctc ccg atc ctc ccc atc atc tcg caa gga tac tca cac atc tac gat        672
Leu Pro Ile Leu Pro Ile Ile Ser Gln Gly Tyr Ser His Ile Tyr Asp           
    210                 215                 220                           

tcg tca aaa cgc tac ttc ccc ggt gga gag ctc gag atc aga gtc ctg        720
Ser Ser Lys Arg Tyr Phe Pro Gly Gly Glu Leu Glu Ile Arg Val Leu           
225                 230                 235                 240           

gaa cct atc ccc acc acg gga ttg acc aca gac gat gtg aac gac ctg        768
Glu Pro Ile Pro Thr Thr Gly Leu Thr Thr Asp Asp Val Asn Asp Leu           
                245                 250                 255               

atg gac aag act cgc aac ctg atg ctg aag cac ctc aag gag atg gat        816
Met Asp Lys Thr Arg Asn Leu Met Leu Lys His Leu Lys Glu Met Asp           
            260                 265                 270                   

tct caa tac tcc tcc tcc acc gct gaa aac gga tcg acc cat att gac        864
Ser Gln Tyr Ser Ser Ser Thr Ala Glu Asn Gly Ser Thr His Ile Asp           
        275                 280                 285                       

gcc gat atc gca aag tca act gcc aca tcg atc gga aac acg gac gat        912
Ala Asp Ile Ala Lys Ser Thr Ala Thr Ser Ile Gly Asn Thr Asp Asp           
    290                 295                 300                           

gct atc aca aag agg agg aca cca aaa gag tag                            945
Ala Ile Thr Lys Arg Arg Thr Pro Lys Glu                                   
305                 310                                                   


<210>  29
<211>  314
<212>  PRT
<213>  Mortierella alpina

<400>  29

Met Ser Ile Gly Ser Ser Asn Pro Val Leu Leu Ala Ala Ile Pro Phe 
1               5                   10                  15      


Val Tyr Leu Phe Val Leu Pro Arg Val Leu Ala Phe Leu Pro Gln Lys 
            20                  25                  30          


Ala Gln Phe Leu Ala Lys Cys Ile Val Val Leu Ile Ala Thr Leu Ile 
        35                  40                  45              


Met Ser Val Ala Gly Cys Phe Ile Ser Ile Val Cys Ala Leu Leu Asp 
    50                  55                  60                  


Lys Arg Tyr Val Ile Asn Tyr Val Val Ser Arg Leu Phe Ser Phe Leu 
65                  70                  75                  80  


Ala Ala Arg Pro Cys Gly Val Thr Tyr Lys Ile Val Gly Glu Glu His 
                85                  90                  95      


Leu Asp Lys Tyr Pro Ala Ile Val Val Cys Asn His Gln Ser Ser Met 
            100                 105                 110         


Asp Met Met Val Leu Gly Arg Val Phe Pro Lys His Cys Val Val Met 
        115                 120                 125             


Ala Lys Lys Glu Leu Leu Tyr Phe Pro Phe Leu Gly Met Phe Met Lys 
    130                 135                 140                 


Leu Ser Asn Ala Ile Phe Ile Asp Arg Lys Asn His Lys Lys Ala Ile 
145                 150                 155                 160 


Glu Ser Thr Thr Gln Ala Val Ala Asp Met Lys Lys His Asn Ser Gly 
                165                 170                 175     


Ile Trp Ile Phe Pro Glu Gly Thr Arg Ser Arg Leu Asp Lys Ala Asp 
            180                 185                 190         


Leu Leu Pro Phe Lys Lys Gly Ala Phe His Leu Ala Ile Gln Ala Gln 
        195                 200                 205             


Leu Pro Ile Leu Pro Ile Ile Ser Gln Gly Tyr Ser His Ile Tyr Asp 
    210                 215                 220                 


Ser Ser Lys Arg Tyr Phe Pro Gly Gly Glu Leu Glu Ile Arg Val Leu 
225                 230                 235                 240 


Glu Pro Ile Pro Thr Thr Gly Leu Thr Thr Asp Asp Val Asn Asp Leu 
                245                 250                 255     


Met Asp Lys Thr Arg Asn Leu Met Leu Lys His Leu Lys Glu Met Asp 
            260                 265                 270         


Ser Gln Tyr Ser Ser Ser Thr Ala Glu Asn Gly Ser Thr His Ile Asp 
        275                 280                 285             


Ala Asp Ile Ala Lys Ser Thr Ala Thr Ser Ile Gly Asn Thr Asp Asp 
    290                 295                 300                 


Ala Ile Thr Lys Arg Arg Thr Pro Lys Glu 
305                 310                 


<210>  30
<211>  1549
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (501)..(1349)
<223>  LPAAT1

<300>
<302>  High eicosapentaenoic acid producing strains of Yarrowia 
       lipolytica
<310>  US-2006-0115881-A1
<311>  2005-11-02
<312>  2006-06-01
<313>  (1)..(1549)

<300>
<302>  High eicosapentaenoic acid producing strains of Yarrowia 
       lipolytica
<310>  WO 2006/052870
<311>  2005-11-03
<312>  2006-05-18
<313>  (1)..(1549)

<400>  30
cacagcataa taccacggca tgaccccgct gactccaacc ttcatttcgg cacatgtagg       60

tgcacaaggg acttcaagag gggccaattt catgcggaca catggcgcaa aaaacgcccg      120

actttgatta cacagacacg taataacgac gaagccgaga tgagcacacg tggccaagtc      180

tgccaatggc cccctggacc cccctgacaa agtttcccaa caagcccagc cgtgcatggt      240

gtgtttttgt gcggagacac acgccaatta ggctcatttg agggtatgca gcgaaaaaaa      300

attagtgtgg gtagtttgtt tgcaggaatc aagtgggtgg ttgaaaaaca agaaagagcg      360

acgacaagag agagagaaaa agagagagag actccataaa gcgtgcatca aaattaaggt      420

gtgtgactat ccgaaaacca aacatgaaca gttggatata tgtcgctgtg attgcagttg      480

ctgccgttct cattgcccga atg tcc gtt gca tcc aag ctc gtc ttc tac gtc      533
                      Met Ser Val Ala Ser Lys Leu Val Phe Tyr Val         
                      1               5                   10              

cgc gcc gcc atc gcc gtg gtc atc ttt gcc gcc tgt gcc acc tac ggc        581
Arg Ala Ala Ile Ala Val Val Ile Phe Ala Ala Cys Ala Thr Tyr Gly           
            15                  20                  25                    

gtg ctg gcg tcc acc att ctc acc gcc atc ggc aag cag ggc ctg gcc        629
Val Leu Ala Ser Thr Ile Leu Thr Ala Ile Gly Lys Gln Gly Leu Ala           
        30                  35                  40                        

caa tgg acc gtt gcc aga gcc ttc tac tac tcg gtg cgc atc ttc ctg        677
Gln Trp Thr Val Ala Arg Ala Phe Tyr Tyr Ser Val Arg Ile Phe Leu           
    45                  50                  55                            

ggt atc agc atc aag ctg cgt agc cgg cag gtg acc gga acc gcc ggt        725
Gly Ile Ser Ile Lys Leu Arg Ser Arg Gln Val Thr Gly Thr Ala Gly           
60                  65                  70                  75            

ctg gat gcc tcc aag atc cag gtc gcc aac acc acc aag ccc att gac        773
Leu Asp Ala Ser Lys Ile Gln Val Ala Asn Thr Thr Lys Pro Ile Asp           
                80                  85                  90                

gac atc acc aaa cac ctg ccc cga cca tgc att ctg att tcc aac cac        821
Asp Ile Thr Lys His Leu Pro Arg Pro Cys Ile Leu Ile Ser Asn His           
            95                  100                 105                   

cag aac gaa atg gac att ctg gtg ctc ggt cgc atc ttc ccc cag tac        869
Gln Asn Glu Met Asp Ile Leu Val Leu Gly Arg Ile Phe Pro Gln Tyr           
        110                 115                 120                       

tgc tcc gtc acc gcc aaa aag gcc ctc aag tgg tac cct ctg ctg ggc        917
Cys Ser Val Thr Ala Lys Lys Ala Leu Lys Trp Tyr Pro Leu Leu Gly           
    125                 130                 135                           

cag ttc atg gcg ctg tcc ggc acc atc ttc ctg gac cga aag gac cga        965
Gln Phe Met Ala Leu Ser Gly Thr Ile Phe Leu Asp Arg Lys Asp Arg           
140                 145                 150                 155           

acc aag tcc gtg cag acc ctc ggc ggc gcc gtc aag acc atc cag agc       1013
Thr Lys Ser Val Gln Thr Leu Gly Gly Ala Val Lys Thr Ile Gln Ser           
                160                 165                 170               

ggc aac gga ggc aag ggc cag agc gtc ttc atg ttc ccc gag gga acc       1061
Gly Asn Gly Gly Lys Gly Gln Ser Val Phe Met Phe Pro Glu Gly Thr           
            175                 180                 185                   

cga tcc tac tcc aag gac gtc ggc atc atg ccc ttc aag aag ggc tgt       1109
Arg Ser Tyr Ser Lys Asp Val Gly Ile Met Pro Phe Lys Lys Gly Cys           
        190                 195                 200                       

ttc cac ctg gcg gtc cag tcg ggc gct ccc att gtc ccc gtg gtg gtc       1157
Phe His Leu Ala Val Gln Ser Gly Ala Pro Ile Val Pro Val Val Val           
    205                 210                 215                           

cag aac acc tcc cga atg ttt tct ttc ggc cga ggc aag ctg gac gcc       1205
Gln Asn Thr Ser Arg Met Phe Ser Phe Gly Arg Gly Lys Leu Asp Ala           
220                 225                 230                 235           

gga gag atc ctt gtc gac gtc ctg agc ccc att gag acc aag ggt ctg       1253
Gly Glu Ile Leu Val Asp Val Leu Ser Pro Ile Glu Thr Lys Gly Leu           
                240                 245                 250               

gac gcc agc aac gtc gac gct ctc atg gcc acc act tat aag gcc atg       1301
Asp Ala Ser Asn Val Asp Ala Leu Met Ala Thr Thr Tyr Lys Ala Met           
            255                 260                 265                   

tgc gag act gcc gac cag att ggc tac gct ggc cag aag act cag tag       1349
Cys Glu Thr Ala Asp Gln Ile Gly Tyr Ala Gly Gln Lys Thr Gln               
        270                 275                 280                       

agactgcagc acaagaagtg cttgtagcta ctttaggaga gagataggta atatgaaaca     1409

tttttcagat cgacacccac ggcgaaccat tggctgtgga gctatgggtg aatggattaa     1469

tatagcaacg aaatctacct cgattaccaa cgcaaaacga gcccactttc tctgtactgt     1529

gctatatcgt gtatacccca                                                 1549


<210>  31
<211>  282
<212>  PRT
<213>  Yarrowia lipolytica

<400>  31

Met Ser Val Ala Ser Lys Leu Val Phe Tyr Val Arg Ala Ala Ile Ala 
1               5                   10                  15      


Val Val Ile Phe Ala Ala Cys Ala Thr Tyr Gly Val Leu Ala Ser Thr 
            20                  25                  30          


Ile Leu Thr Ala Ile Gly Lys Gln Gly Leu Ala Gln Trp Thr Val Ala 
        35                  40                  45              


Arg Ala Phe Tyr Tyr Ser Val Arg Ile Phe Leu Gly Ile Ser Ile Lys 
    50                  55                  60                  


Leu Arg Ser Arg Gln Val Thr Gly Thr Ala Gly Leu Asp Ala Ser Lys 
65                  70                  75                  80  


Ile Gln Val Ala Asn Thr Thr Lys Pro Ile Asp Asp Ile Thr Lys His 
                85                  90                  95      


Leu Pro Arg Pro Cys Ile Leu Ile Ser Asn His Gln Asn Glu Met Asp 
            100                 105                 110         


Ile Leu Val Leu Gly Arg Ile Phe Pro Gln Tyr Cys Ser Val Thr Ala 
        115                 120                 125             


Lys Lys Ala Leu Lys Trp Tyr Pro Leu Leu Gly Gln Phe Met Ala Leu 
    130                 135                 140                 


Ser Gly Thr Ile Phe Leu Asp Arg Lys Asp Arg Thr Lys Ser Val Gln 
145                 150                 155                 160 


Thr Leu Gly Gly Ala Val Lys Thr Ile Gln Ser Gly Asn Gly Gly Lys 
                165                 170                 175     


Gly Gln Ser Val Phe Met Phe Pro Glu Gly Thr Arg Ser Tyr Ser Lys 
            180                 185                 190         


Asp Val Gly Ile Met Pro Phe Lys Lys Gly Cys Phe His Leu Ala Val 
        195                 200                 205             


Gln Ser Gly Ala Pro Ile Val Pro Val Val Val Gln Asn Thr Ser Arg 
    210                 215                 220                 


Met Phe Ser Phe Gly Arg Gly Lys Leu Asp Ala Gly Glu Ile Leu Val 
225                 230                 235                 240 


Asp Val Leu Ser Pro Ile Glu Thr Lys Gly Leu Asp Ala Ser Asn Val 
                245                 250                 255     


Asp Ala Leu Met Ala Thr Thr Tyr Lys Ala Met Cys Glu Thr Ala Asp 
            260                 265                 270         


Gln Ile Gly Tyr Ala Gly Gln Lys Thr Gln 
        275                 280         


<210>  32
<211>  303
<212>  PRT
<213>  Saccharomyces cerevisiae


<220>
<221>  MISC_FEATURE
<222>  (1)..(303)
<223>  Slc1p; GenBank Accession No.  NP_010231

<400>  32

Met Ser Val Ile Gly Arg Phe Leu Tyr Tyr Leu Arg Ser Val Leu Val 
1               5                   10                  15      


Val Leu Ala Leu Ala Gly Cys Gly Phe Tyr Gly Val Ile Ala Ser Ile 
            20                  25                  30          


Leu Cys Thr Leu Ile Gly Lys Gln His Leu Ala Gln Trp Ile Thr Ala 
        35                  40                  45              


Arg Cys Phe Tyr His Val Met Lys Leu Met Leu Gly Leu Asp Val Lys 
    50                  55                  60                  


Val Val Gly Glu Glu Asn Leu Ala Lys Lys Pro Tyr Ile Met Ile Ala 
65                  70                  75                  80  


Asn His Gln Ser Thr Leu Asp Ile Phe Met Leu Gly Arg Ile Phe Pro 
                85                  90                  95      


Pro Gly Cys Thr Val Thr Ala Lys Lys Ser Leu Lys Tyr Val Pro Phe 
            100                 105                 110         


Leu Gly Trp Phe Met Ala Leu Ser Gly Thr Tyr Phe Leu Asp Arg Ser 
        115                 120                 125             


Lys Arg Gln Glu Ala Ile Asp Thr Leu Asn Lys Gly Leu Glu Asn Val 
    130                 135                 140                 


Lys Lys Asn Lys Arg Ala Leu Trp Val Phe Pro Glu Gly Thr Arg Ser 
145                 150                 155                 160 


Tyr Thr Ser Glu Leu Thr Met Leu Pro Phe Lys Lys Gly Ala Phe His 
                165                 170                 175     


Leu Ala Gln Gln Gly Lys Ile Pro Ile Val Pro Val Val Val Ser Asn 
            180                 185                 190         


Thr Ser Thr Leu Val Ser Pro Lys Tyr Gly Val Phe Asn Arg Gly Cys 
        195                 200                 205             


Met Ile Val Arg Ile Leu Lys Pro Ile Ser Thr Glu Asn Leu Thr Lys 
    210                 215                 220                 


Asp Lys Ile Gly Glu Phe Ala Glu Lys Val Arg Asp Gln Met Val Asp 
225                 230                 235                 240 


Thr Leu Lys Glu Ile Gly Tyr Ser Pro Ala Ile Asn Asp Thr Thr Leu 
                245                 250                 255     


Pro Pro Gln Ala Ile Glu Tyr Ala Ala Leu Gln His Asp Lys Lys Val 
            260                 265                 270         


Asn Lys Lys Ile Lys Asn Glu Pro Val Pro Ser Val Ser Ile Ser Asn 
        275                 280                 285             


Asp Val Asn Thr His Asn Glu Gly Ser Ser Val Lys Lys Met His 
    290                 295                 300             


<210>  33
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-acyl-sn-glycerol-3-phosphate acyltransferase motif


<220>
<221>  misc_feature
<222>  (3)..(6)
<223>  Xaa can be any naturally occurring amino acid

<400>  33

Asn His Xaa Xaa Xaa Xaa Asp 
1               5           


<210>  34
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-acyl-sn-glycerol-3-phosphate acyltransferase motif

<400>  34

Glu Gly Thr Arg 
1               


<210>  35
<211>  955
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (3)..(947)
<223>  synthetic LPAAT1 (codon-optimized for Yarrowia lipolytica)

<400>  35
ac atg tct att ggt tcg tcc aac ccc gtg ctc ttg gct gcg att ccc          47
   Met Ser Ile Gly Ser Ser Asn Pro Val Leu Leu Ala Ala Ile Pro            
   1               5                   10                  15             

ttc gtc tac ctg ttt gtc ctc cca cga gtc ctg gct ttc ctg cct cag         95
Phe Val Tyr Leu Phe Val Leu Pro Arg Val Leu Ala Phe Leu Pro Gln           
                20                  25                  30                

aag gct cag ttc ctg gcc aaa tgt att gtg gtc ctg att gcc acg ctt        143
Lys Ala Gln Phe Leu Ala Lys Cys Ile Val Val Leu Ile Ala Thr Leu           
            35                  40                  45                    

atc atg tcc gtt gca ggc tgc ttc atc tcg atc gtg tgc gct ctt ctg        191
Ile Met Ser Val Ala Gly Cys Phe Ile Ser Ile Val Cys Ala Leu Leu           
        50                  55                  60                        

gac aag aga tac gtc atc aat tac gtt gtg tcg cga ttg ttc tcc ttc        239
Asp Lys Arg Tyr Val Ile Asn Tyr Val Val Ser Arg Leu Phe Ser Phe           
    65                  70                  75                            

ctt gcc gct cga ccg tgt ggt gtg acc tat aag att gtt ggt gag gaa        287
Leu Ala Ala Arg Pro Cys Gly Val Thr Tyr Lys Ile Val Gly Glu Glu           
80                  85                  90                  95            

cac ctc gat aag tac cct gct atc gtg gtc tgt aac cat caa tcc tct        335
His Leu Asp Lys Tyr Pro Ala Ile Val Val Cys Asn His Gln Ser Ser           
                100                 105                 110               

atg gat atg atg gtt ttg gga cga gtt ttt cca aag cac tgc gtt gtc        383
Met Asp Met Met Val Leu Gly Arg Val Phe Pro Lys His Cys Val Val           
            115                 120                 125                   

atg gcg aag aag gaa ctc ctg tac ttt ccc ttt ttg gga atg ttt atg        431
Met Ala Lys Lys Glu Leu Leu Tyr Phe Pro Phe Leu Gly Met Phe Met           
        130                 135                 140                       

aaa ctg agc aac gct atc ttc atc gac cgg aag aac cac aag aaa gcc        479
Lys Leu Ser Asn Ala Ile Phe Ile Asp Arg Lys Asn His Lys Lys Ala           
    145                 150                 155                           

atc gag tct acc acc caa gcc gtg gcg gac atg aag aag cac aac tct        527
Ile Glu Ser Thr Thr Gln Ala Val Ala Asp Met Lys Lys His Asn Ser           
160                 165                 170                 175           

gga atc tgg att ttc cca gag ggc acc cgg tct aga ctg gac aag gca        575
Gly Ile Trp Ile Phe Pro Glu Gly Thr Arg Ser Arg Leu Asp Lys Ala           
                180                 185                 190               

gac ctg ctg ccc ttc aag aaa ggt gcc ttt cat ctt gca att cag gcc        623
Asp Leu Leu Pro Phe Lys Lys Gly Ala Phe His Leu Ala Ile Gln Ala           
            195                 200                 205                   

cag ctc cct att ctc ccc att atc tcg cag ggc tat tcc cat atc tac        671
Gln Leu Pro Ile Leu Pro Ile Ile Ser Gln Gly Tyr Ser His Ile Tyr           
        210                 215                 220                       

gac tct tcg aag cgg tac ttc ccc ggt gga gag ctc gag atc aga gtc        719
Asp Ser Ser Lys Arg Tyr Phe Pro Gly Gly Glu Leu Glu Ile Arg Val           
    225                 230                 235                           

ctg gag ccc att cct aca act ggc ctc act act gat gat gtg aac gac        767
Leu Glu Pro Ile Pro Thr Thr Gly Leu Thr Thr Asp Asp Val Asn Asp           
240                 245                 250                 255           

ctg atg gac aag aca cga aac ctt atg ctc aag cac ttg aag gag atg        815
Leu Met Asp Lys Thr Arg Asn Leu Met Leu Lys His Leu Lys Glu Met           
                260                 265                 270               

gat tcc cag tat tcg tcg agc act gct gaa aat gga tcc acg cac atc        863
Asp Ser Gln Tyr Ser Ser Ser Thr Ala Glu Asn Gly Ser Thr His Ile           
            275                 280                 285                   

gac gcc gat att gcc aag tct aca gcc acc agc att ggc aac act gac        911
Asp Ala Asp Ile Ala Lys Ser Thr Ala Thr Ser Ile Gly Asn Thr Asp           
        290                 295                 300                       

gac gca att aca aaa cgt cgt acc cct aag gaa taa gcggccgc               955
Asp Ala Ile Thr Lys Arg Arg Thr Pro Lys Glu                               
    305                 310                                               


<210>  36
<211>  314
<212>  PRT
<213>  Mortierella alpina

<400>  36

Met Ser Ile Gly Ser Ser Asn Pro Val Leu Leu Ala Ala Ile Pro Phe 
1               5                   10                  15      


Val Tyr Leu Phe Val Leu Pro Arg Val Leu Ala Phe Leu Pro Gln Lys 
            20                  25                  30          


Ala Gln Phe Leu Ala Lys Cys Ile Val Val Leu Ile Ala Thr Leu Ile 
        35                  40                  45              


Met Ser Val Ala Gly Cys Phe Ile Ser Ile Val Cys Ala Leu Leu Asp 
    50                  55                  60                  


Lys Arg Tyr Val Ile Asn Tyr Val Val Ser Arg Leu Phe Ser Phe Leu 
65                  70                  75                  80  


Ala Ala Arg Pro Cys Gly Val Thr Tyr Lys Ile Val Gly Glu Glu His 
                85                  90                  95      


Leu Asp Lys Tyr Pro Ala Ile Val Val Cys Asn His Gln Ser Ser Met 
            100                 105                 110         


Asp Met Met Val Leu Gly Arg Val Phe Pro Lys His Cys Val Val Met 
        115                 120                 125             


Ala Lys Lys Glu Leu Leu Tyr Phe Pro Phe Leu Gly Met Phe Met Lys 
    130                 135                 140                 


Leu Ser Asn Ala Ile Phe Ile Asp Arg Lys Asn His Lys Lys Ala Ile 
145                 150                 155                 160 


Glu Ser Thr Thr Gln Ala Val Ala Asp Met Lys Lys His Asn Ser Gly 
                165                 170                 175     


Ile Trp Ile Phe Pro Glu Gly Thr Arg Ser Arg Leu Asp Lys Ala Asp 
            180                 185                 190         


Leu Leu Pro Phe Lys Lys Gly Ala Phe His Leu Ala Ile Gln Ala Gln 
        195                 200                 205             


Leu Pro Ile Leu Pro Ile Ile Ser Gln Gly Tyr Ser His Ile Tyr Asp 
    210                 215                 220                 


Ser Ser Lys Arg Tyr Phe Pro Gly Gly Glu Leu Glu Ile Arg Val Leu 
225                 230                 235                 240 


Glu Pro Ile Pro Thr Thr Gly Leu Thr Thr Asp Asp Val Asn Asp Leu 
                245                 250                 255     


Met Asp Lys Thr Arg Asn Leu Met Leu Lys His Leu Lys Glu Met Asp 
            260                 265                 270         


Ser Gln Tyr Ser Ser Ser Thr Ala Glu Asn Gly Ser Thr His Ile Asp 
        275                 280                 285             


Ala Asp Ile Ala Lys Ser Thr Ala Thr Ser Ile Gly Asn Thr Asp Asp 
    290                 295                 300                 


Ala Ile Thr Lys Arg Arg Thr Pro Lys Glu 
305                 310                 


<210>  37
<211>  1185
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1185)
<223>  diacylglycerol cholinephosphotransferase (YlCPT1)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF
<310>  WO 2006/052870
<311>  2005-11-03
<312>  2006-05-18
<313>  (1)..(1185)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF
<310>  US 2006-0115881-A1
<311>  2005-11-02
<312>  2006-06-01
<313>  (1)..(1185)

<400>  37
atg ggc gta ttc att aaa cag gag cag ctt ccg gct ctc aag aag tac         48
Met Gly Val Phe Ile Lys Gln Glu Gln Leu Pro Ala Leu Lys Lys Tyr           
1               5                   10                  15                

aag tac tcc gcc gag gat cac tcg ttc atc tcc aac aac att ctg cgc         96
Lys Tyr Ser Ala Glu Asp His Ser Phe Ile Ser Asn Asn Ile Leu Arg           
            20                  25                  30                    

ccc ttc tgg cga cag ttt gtc aaa atc ttc cct ctg tgg atg gcc ccc        144
Pro Phe Trp Arg Gln Phe Val Lys Ile Phe Pro Leu Trp Met Ala Pro           
        35                  40                  45                        

aac atg gtg act ctg ttg ggc ttc ttc ttt gtc att gtg aac ttc atc        192
Asn Met Val Thr Leu Leu Gly Phe Phe Phe Val Ile Val Asn Phe Ile           
    50                  55                  60                            

acc atg ctc att gtt gat ccc acc cac gac cgc gag cct ccc aga tgg        240
Thr Met Leu Ile Val Asp Pro Thr His Asp Arg Glu Pro Pro Arg Trp           
65                  70                  75                  80            

gtc tac ctc acc tac gct ctg ggt ctg ttc ctt tac cag aca ttt gat        288
Val Tyr Leu Thr Tyr Ala Leu Gly Leu Phe Leu Tyr Gln Thr Phe Asp           
                85                  90                  95                

gcc tgt gac gga tcc cat gcc cga cga act ggc cag agt gga ccc ctt        336
Ala Cys Asp Gly Ser His Ala Arg Arg Thr Gly Gln Ser Gly Pro Leu           
            100                 105                 110                   

gga gag ctg ttt gac cac tgt gtc gac gcc atg aat acc tct ctg att        384
Gly Glu Leu Phe Asp His Cys Val Asp Ala Met Asn Thr Ser Leu Ile           
        115                 120                 125                       

ctc acg gtg gtg gtg tcc acc acc cat atg gga tat aac atg aag ctg        432
Leu Thr Val Val Val Ser Thr Thr His Met Gly Tyr Asn Met Lys Leu           
    130                 135                 140                           

ctg att gtg cag att gcc gct ctc gga aac ttc tac ctg tcg acc tgg        480
Leu Ile Val Gln Ile Ala Ala Leu Gly Asn Phe Tyr Leu Ser Thr Trp           
145                 150                 155                 160           

gag acc tac cat acc gga act ctg tac ctt tct ggc ttc tct ggt cct        528
Glu Thr Tyr His Thr Gly Thr Leu Tyr Leu Ser Gly Phe Ser Gly Pro           
                165                 170                 175               

gtt gaa ggt atc ttg att ctg gtg gct ctt ttc gtc ctc acc ttc ttc        576
Val Glu Gly Ile Leu Ile Leu Val Ala Leu Phe Val Leu Thr Phe Phe           
            180                 185                 190                   

act ggt ccc aac gtg tac gct ctg acc gtc tac gag gct ctt ccc gaa        624
Thr Gly Pro Asn Val Tyr Ala Leu Thr Val Tyr Glu Ala Leu Pro Glu           
        195                 200                 205                       

tcc atc act tcg ctg ctg cct gcc agc ttc ctg gac gtc acc atc acc        672
Ser Ile Thr Ser Leu Leu Pro Ala Ser Phe Leu Asp Val Thr Ile Thr           
    210                 215                 220                           

cag atc tac att gga ttc gga gtg ctg ggc atg gtg ttc aac atc tac        720
Gln Ile Tyr Ile Gly Phe Gly Val Leu Gly Met Val Phe Asn Ile Tyr           
225                 230                 235                 240           

ggc gcc tgc gga aac gtg atc aag tac tac aac aac aag ggc aag agc        768
Gly Ala Cys Gly Asn Val Ile Lys Tyr Tyr Asn Asn Lys Gly Lys Ser           
                245                 250                 255               

gct ctc ccc gcc att ctc gga atc gcc ccc ttt ggc atc ttc tac gtc        816
Ala Leu Pro Ala Ile Leu Gly Ile Ala Pro Phe Gly Ile Phe Tyr Val           
            260                 265                 270                   

ggc gtc ttt gcc tgg gcc cat gtt gct cct ctg ctt ctc tcc aag tac        864
Gly Val Phe Ala Trp Ala His Val Ala Pro Leu Leu Leu Ser Lys Tyr           
        275                 280                 285                       

gcc atc gtc tat ctg ttt gcc att ggg gct gcc ttt gcc atg caa gtc        912
Ala Ile Val Tyr Leu Phe Ala Ile Gly Ala Ala Phe Ala Met Gln Val           
    290                 295                 300                           

ggc cag atg att ctt gcc cat ctc gtg ctt gct ccc ttc ccc cac tgg        960
Gly Gln Met Ile Leu Ala His Leu Val Leu Ala Pro Phe Pro His Trp           
305                 310                 315                 320           

aac gtg ctg ctc ttc ttc ccc ttt gtg gga ctg gca gtg cac tac att       1008
Asn Val Leu Leu Phe Phe Pro Phe Val Gly Leu Ala Val His Tyr Ile           
                325                 330                 335               

gca ccc gtg ttt ggc tgg gac gcc gat atc gtg tcg gtt aac act ctc       1056
Ala Pro Val Phe Gly Trp Asp Ala Asp Ile Val Ser Val Asn Thr Leu           
            340                 345                 350                   

ttc acc tgt ttt ggc gcc acc ctc tcc att tac gcc ttc ttt gtg ctt       1104
Phe Thr Cys Phe Gly Ala Thr Leu Ser Ile Tyr Ala Phe Phe Val Leu           
        355                 360                 365                       

gag atc atc gac gag atc acc aac tac ctc gat atc tgg tgt ctg cga       1152
Glu Ile Ile Asp Glu Ile Thr Asn Tyr Leu Asp Ile Trp Cys Leu Arg           
    370                 375                 380                           

atc aag tac cct cag gag aag aag act gag taa                           1185
Ile Lys Tyr Pro Gln Glu Lys Lys Thr Glu                                   
385                 390                                                   


<210>  38
<211>  394
<212>  PRT
<213>  Yarrowia lipolytica

<400>  38

Met Gly Val Phe Ile Lys Gln Glu Gln Leu Pro Ala Leu Lys Lys Tyr 
1               5                   10                  15      


Lys Tyr Ser Ala Glu Asp His Ser Phe Ile Ser Asn Asn Ile Leu Arg 
            20                  25                  30          


Pro Phe Trp Arg Gln Phe Val Lys Ile Phe Pro Leu Trp Met Ala Pro 
        35                  40                  45              


Asn Met Val Thr Leu Leu Gly Phe Phe Phe Val Ile Val Asn Phe Ile 
    50                  55                  60                  


Thr Met Leu Ile Val Asp Pro Thr His Asp Arg Glu Pro Pro Arg Trp 
65                  70                  75                  80  


Val Tyr Leu Thr Tyr Ala Leu Gly Leu Phe Leu Tyr Gln Thr Phe Asp 
                85                  90                  95      


Ala Cys Asp Gly Ser His Ala Arg Arg Thr Gly Gln Ser Gly Pro Leu 
            100                 105                 110         


Gly Glu Leu Phe Asp His Cys Val Asp Ala Met Asn Thr Ser Leu Ile 
        115                 120                 125             


Leu Thr Val Val Val Ser Thr Thr His Met Gly Tyr Asn Met Lys Leu 
    130                 135                 140                 


Leu Ile Val Gln Ile Ala Ala Leu Gly Asn Phe Tyr Leu Ser Thr Trp 
145                 150                 155                 160 


Glu Thr Tyr His Thr Gly Thr Leu Tyr Leu Ser Gly Phe Ser Gly Pro 
                165                 170                 175     


Val Glu Gly Ile Leu Ile Leu Val Ala Leu Phe Val Leu Thr Phe Phe 
            180                 185                 190         


Thr Gly Pro Asn Val Tyr Ala Leu Thr Val Tyr Glu Ala Leu Pro Glu 
        195                 200                 205             


Ser Ile Thr Ser Leu Leu Pro Ala Ser Phe Leu Asp Val Thr Ile Thr 
    210                 215                 220                 


Gln Ile Tyr Ile Gly Phe Gly Val Leu Gly Met Val Phe Asn Ile Tyr 
225                 230                 235                 240 


Gly Ala Cys Gly Asn Val Ile Lys Tyr Tyr Asn Asn Lys Gly Lys Ser 
                245                 250                 255     


Ala Leu Pro Ala Ile Leu Gly Ile Ala Pro Phe Gly Ile Phe Tyr Val 
            260                 265                 270         


Gly Val Phe Ala Trp Ala His Val Ala Pro Leu Leu Leu Ser Lys Tyr 
        275                 280                 285             


Ala Ile Val Tyr Leu Phe Ala Ile Gly Ala Ala Phe Ala Met Gln Val 
    290                 295                 300                 


Gly Gln Met Ile Leu Ala His Leu Val Leu Ala Pro Phe Pro His Trp 
305                 310                 315                 320 


Asn Val Leu Leu Phe Phe Pro Phe Val Gly Leu Ala Val His Tyr Ile 
                325                 330                 335     


Ala Pro Val Phe Gly Trp Asp Ala Asp Ile Val Ser Val Asn Thr Leu 
            340                 345                 350         


Phe Thr Cys Phe Gly Ala Thr Leu Ser Ile Tyr Ala Phe Phe Val Leu 
        355                 360                 365             


Glu Ile Ile Asp Glu Ile Thr Asn Tyr Leu Asp Ile Trp Cys Leu Arg 
    370                 375                 380                 


Ile Lys Tyr Pro Gln Glu Lys Lys Thr Glu 
385                 390                 


<210>  39
<211>  1515
<212>  DNA
<213>  Rhizobium leguminosarum bv. viciae 3841


<220>
<221>  CDS
<222>  (1)..(1515)
<223>  GenBank Accession No. YP_766603

<400>  39
gtg agc aac cat ctt ttc gac gcc atg cgg gct gcc gcg ccc ggt gac         48
Val Ser Asn His Leu Phe Asp Ala Met Arg Ala Ala Ala Pro Gly Asp           
1               5                   10                  15                

gca ccg ttc atc cgg atc gat aac gcg cgc aca tgg acc tat gat gac         96
Ala Pro Phe Ile Arg Ile Asp Asn Ala Arg Thr Trp Thr Tyr Asp Asp           
            20                  25                  30                    

gcg atc gct ctt tcc ggc cgt att gcc ggc gcg atg gac gcg ctc ggc        144
Ala Ile Ala Leu Ser Gly Arg Ile Ala Gly Ala Met Asp Ala Leu Gly           
        35                  40                  45                        

att cgc ccc ggc gac cgg gtg gcg gtg cag gtc gag aaa agt gcc gag        192
Ile Arg Pro Gly Asp Arg Val Ala Val Gln Val Glu Lys Ser Ala Glu           
    50                  55                  60                            

gcg ttg atc ctc tat ctc gcc tgt ctt cga acc ggc gcc gtc tac ctg        240
Ala Leu Ile Leu Tyr Leu Ala Cys Leu Arg Thr Gly Ala Val Tyr Leu           
65                  70                  75                  80            

ccg ctc aac acc gcc tat acg ctg gct gag ctc gat tac ttt atc ggc        288
Pro Leu Asn Thr Ala Tyr Thr Leu Ala Glu Leu Asp Tyr Phe Ile Gly           
                85                  90                  95                

gat gcg gag ccg cgt ttg gtg gtg gtt gcg ccg gcg gct cga ggc ggc        336
Asp Ala Glu Pro Arg Leu Val Val Val Ala Pro Ala Ala Arg Gly Gly           
            100                 105                 110                   

gtg gag aca atc gcc aag cgc cac ggc gcg atc gtc gaa acg ctc gat        384
Val Glu Thr Ile Ala Lys Arg His Gly Ala Ile Val Glu Thr Leu Asp           
        115                 120                 125                       

gct gat ggc cgc ggc tca ttg ctg gat ctc gca cgc gat gag ccg gcc        432
Ala Asp Gly Arg Gly Ser Leu Leu Asp Leu Ala Arg Asp Glu Pro Ala           
    130                 135                 140                           

gac ttt gtc gat gcc tcg cgt tcc gcg gat gat ctg gcc gcg atc ctc        480
Asp Phe Val Asp Ala Ser Arg Ser Ala Asp Asp Leu Ala Ala Ile Leu           
145                 150                 155                 160           

tac acg tcg gga acg acc gga cgc tcc aag ggg gcg atg ctc acg cat        528
Tyr Thr Ser Gly Thr Thr Gly Arg Ser Lys Gly Ala Met Leu Thr His           
                165                 170                 175               

ggg aac ctg ctc tcg aac gcc ctg acc ttg cga gac tat tgg cgc gtc        576
Gly Asn Leu Leu Ser Asn Ala Leu Thr Leu Arg Asp Tyr Trp Arg Val           
            180                 185                 190                   

acc gct gac gat cgg ctg atc cat gcc ttg ccg atc ttc cac acg cat        624
Thr Ala Asp Asp Arg Leu Ile His Ala Leu Pro Ile Phe His Thr His           
        195                 200                 205                       

ggg ctg ttc gtc gcc acg aac gtc aca ctg ctt gcc ggc gcc tcg atg        672
Gly Leu Phe Val Ala Thr Asn Val Thr Leu Leu Ala Gly Ala Ser Met           
    210                 215                 220                           

ttc ctg ctg tcg aaa ttc gac gcc gac gag gtc gtt tca ctg atg cca        720
Phe Leu Leu Ser Lys Phe Asp Ala Asp Glu Val Val Ser Leu Met Pro           
225                 230                 235                 240           

cag gca act atg ctg atg ggc gtg ccg acc ttc tac gtg cgc ctc ctg        768
Gln Ala Thr Met Leu Met Gly Val Pro Thr Phe Tyr Val Arg Leu Leu           
                245                 250                 255               

caa agc ccg cgc ctc gaa aaa ggg gcg gtc gcc agc atc cgc ctc ttc        816
Gln Ser Pro Arg Leu Glu Lys Gly Ala Val Ala Ser Ile Arg Leu Phe           
            260                 265                 270                   

att tcc ggt tcg gct ccc ctg ctg gcc gaa acc cat gcc gag ttc cat        864
Ile Ser Gly Ser Ala Pro Leu Leu Ala Glu Thr His Ala Glu Phe His           
        275                 280                 285                       

gcg cgt acc ggt cac gcc att ctc gag cgc tac ggc atg acg gaa acc        912
Ala Arg Thr Gly His Ala Ile Leu Glu Arg Tyr Gly Met Thr Glu Thr           
    290                 295                 300                           

aat atg aac acg tcc aac ccc tat gag ggc aaa cgg att gcc gga acg        960
Asn Met Asn Thr Ser Asn Pro Tyr Glu Gly Lys Arg Ile Ala Gly Thr           
305                 310                 315                 320           

gtt ggt ttc ccg ctg cct gat gtg acg gtg cgc gtc acc gat ccc gcc       1008
Val Gly Phe Pro Leu Pro Asp Val Thr Val Arg Val Thr Asp Pro Ala           
                325                 330                 335               

acc ggg ctc gtg ctg cca cct gaa gag acg ggc atg atc gag atc aag       1056
Thr Gly Leu Val Leu Pro Pro Glu Glu Thr Gly Met Ile Glu Ile Lys           
            340                 345                 350                   

gga ccg aac gtc ttc aag ggc tat tgg cgc atg ccg gaa aag acc gcg       1104
Gly Pro Asn Val Phe Lys Gly Tyr Trp Arg Met Pro Glu Lys Thr Ala           
        355                 360                 365                       

gcc gaa ttc acc gcc gac ggt ttc ttc atc agc ggc gat ctc ggc aag       1152
Ala Glu Phe Thr Ala Asp Gly Phe Phe Ile Ser Gly Asp Leu Gly Lys           
    370                 375                 380                           

atc gac cgg gaa ggt tat gtc cac atc gtc ggt cgc ggc aag gat ctg       1200
Ile Asp Arg Glu Gly Tyr Val His Ile Val Gly Arg Gly Lys Asp Leu           
385                 390                 395                 400           

gtg atc tcg ggt gga tac aac atc tat ccg aaa gag gtc gaa ggc gag       1248
Val Ile Ser Gly Gly Tyr Asn Ile Tyr Pro Lys Glu Val Glu Gly Glu           
                405                 410                 415               

atc gac cag atc gag ggt gtg gtt gag agc gct gtg atc ggt gtg ccg       1296
Ile Asp Gln Ile Glu Gly Val Val Glu Ser Ala Val Ile Gly Val Pro           
            420                 425                 430                   

cat ccc gat ttc gga gaa ggc gta acg gct gtc gtc gta tgt aag ccc       1344
His Pro Asp Phe Gly Glu Gly Val Thr Ala Val Val Val Cys Lys Pro           
        435                 440                 445                       

ggc gcc gtc ctc gat gaa aag acc atc gtc agc gcc ctc cag gac cgt       1392
Gly Ala Val Leu Asp Glu Lys Thr Ile Val Ser Ala Leu Gln Asp Arg           
    450                 455                 460                           

ctc gcc cgc tac aaa caa ccc aag cgc atc atc ttc gcc gac gac ctg       1440
Leu Ala Arg Tyr Lys Gln Pro Lys Arg Ile Ile Phe Ala Asp Asp Leu           
465                 470                 475                 480           

ccg cgc aac act atg ggt aag gtt cag aag aat atc ctg cgg cag caa       1488
Pro Arg Asn Thr Met Gly Lys Val Gln Lys Asn Ile Leu Arg Gln Gln           
                485                 490                 495               

tac gcc gat ctt tat acc agg agg taa                                   1515
Tyr Ala Asp Leu Tyr Thr Arg Arg                                           
            500                                                           


<210>  40
<211>  504
<212>  PRT
<213>  Rhizobium leguminosarum bv. viciae 3841

<400>  40

Val Ser Asn His Leu Phe Asp Ala Met Arg Ala Ala Ala Pro Gly Asp 
1               5                   10                  15      


Ala Pro Phe Ile Arg Ile Asp Asn Ala Arg Thr Trp Thr Tyr Asp Asp 
            20                  25                  30          


Ala Ile Ala Leu Ser Gly Arg Ile Ala Gly Ala Met Asp Ala Leu Gly 
        35                  40                  45              


Ile Arg Pro Gly Asp Arg Val Ala Val Gln Val Glu Lys Ser Ala Glu 
    50                  55                  60                  


Ala Leu Ile Leu Tyr Leu Ala Cys Leu Arg Thr Gly Ala Val Tyr Leu 
65                  70                  75                  80  


Pro Leu Asn Thr Ala Tyr Thr Leu Ala Glu Leu Asp Tyr Phe Ile Gly 
                85                  90                  95      


Asp Ala Glu Pro Arg Leu Val Val Val Ala Pro Ala Ala Arg Gly Gly 
            100                 105                 110         


Val Glu Thr Ile Ala Lys Arg His Gly Ala Ile Val Glu Thr Leu Asp 
        115                 120                 125             


Ala Asp Gly Arg Gly Ser Leu Leu Asp Leu Ala Arg Asp Glu Pro Ala 
    130                 135                 140                 


Asp Phe Val Asp Ala Ser Arg Ser Ala Asp Asp Leu Ala Ala Ile Leu 
145                 150                 155                 160 


Tyr Thr Ser Gly Thr Thr Gly Arg Ser Lys Gly Ala Met Leu Thr His 
                165                 170                 175     


Gly Asn Leu Leu Ser Asn Ala Leu Thr Leu Arg Asp Tyr Trp Arg Val 
            180                 185                 190         


Thr Ala Asp Asp Arg Leu Ile His Ala Leu Pro Ile Phe His Thr His 
        195                 200                 205             


Gly Leu Phe Val Ala Thr Asn Val Thr Leu Leu Ala Gly Ala Ser Met 
    210                 215                 220                 


Phe Leu Leu Ser Lys Phe Asp Ala Asp Glu Val Val Ser Leu Met Pro 
225                 230                 235                 240 


Gln Ala Thr Met Leu Met Gly Val Pro Thr Phe Tyr Val Arg Leu Leu 
                245                 250                 255     


Gln Ser Pro Arg Leu Glu Lys Gly Ala Val Ala Ser Ile Arg Leu Phe 
            260                 265                 270         


Ile Ser Gly Ser Ala Pro Leu Leu Ala Glu Thr His Ala Glu Phe His 
        275                 280                 285             


Ala Arg Thr Gly His Ala Ile Leu Glu Arg Tyr Gly Met Thr Glu Thr 
    290                 295                 300                 


Asn Met Asn Thr Ser Asn Pro Tyr Glu Gly Lys Arg Ile Ala Gly Thr 
305                 310                 315                 320 


Val Gly Phe Pro Leu Pro Asp Val Thr Val Arg Val Thr Asp Pro Ala 
                325                 330                 335     


Thr Gly Leu Val Leu Pro Pro Glu Glu Thr Gly Met Ile Glu Ile Lys 
            340                 345                 350         


Gly Pro Asn Val Phe Lys Gly Tyr Trp Arg Met Pro Glu Lys Thr Ala 
        355                 360                 365             


Ala Glu Phe Thr Ala Asp Gly Phe Phe Ile Ser Gly Asp Leu Gly Lys 
    370                 375                 380                 


Ile Asp Arg Glu Gly Tyr Val His Ile Val Gly Arg Gly Lys Asp Leu 
385                 390                 395                 400 


Val Ile Ser Gly Gly Tyr Asn Ile Tyr Pro Lys Glu Val Glu Gly Glu 
                405                 410                 415     


Ile Asp Gln Ile Glu Gly Val Val Glu Ser Ala Val Ile Gly Val Pro 
            420                 425                 430         


His Pro Asp Phe Gly Glu Gly Val Thr Ala Val Val Val Cys Lys Pro 
        435                 440                 445             


Gly Ala Val Leu Asp Glu Lys Thr Ile Val Ser Ala Leu Gln Asp Arg 
    450                 455                 460                 


Leu Ala Arg Tyr Lys Gln Pro Lys Arg Ile Ile Phe Ala Asp Asp Leu 
465                 470                 475                 480 


Pro Arg Asn Thr Met Gly Lys Val Gln Lys Asn Ile Leu Arg Gln Gln 
                485                 490                 495     


Tyr Ala Asp Leu Tyr Thr Arg Arg 
            500                 


<210>  41
<211>  1518
<212>  DNA
<213>  Rhizobium leguminosarum bv. viciae 3841


<220>
<221>  CDS
<222>  (1)..(1518)
<223>  synthetic malonyl-CoA synthetase (codon-optimized for Yarrowia 
       lipolytica)

<400>  41
atg gtc tcc aac cac ctg ttc gac gcc atg cga gct gcc gct ccc gga         48
Met Val Ser Asn His Leu Phe Asp Ala Met Arg Ala Ala Ala Pro Gly           
1               5                   10                  15                

gac gca cct ttc att cga atc gac aac gct cgg acc tgg act tac gat         96
Asp Ala Pro Phe Ile Arg Ile Asp Asn Ala Arg Thr Trp Thr Tyr Asp           
            20                  25                  30                    

gac gcc att gct ctt tcc ggt cga atc gct gga gct atg gac gca ctc        144
Asp Ala Ile Ala Leu Ser Gly Arg Ile Ala Gly Ala Met Asp Ala Leu           
        35                  40                  45                        

ggc att cga ccc gga gac aga gtt gcc gtg cag gtc gag aag tct gcc        192
Gly Ile Arg Pro Gly Asp Arg Val Ala Val Gln Val Glu Lys Ser Ala           
    50                  55                  60                            

gag gcg ttg att ctc tac ctg gcc tgt ctt cga acc gga gct gtc tac        240
Glu Ala Leu Ile Leu Tyr Leu Ala Cys Leu Arg Thr Gly Ala Val Tyr           
65                  70                  75                  80            

ctg cct ctc aac act gcc tac acc ctg gcc gag ctc gac tac ttc atc        288
Leu Pro Leu Asn Thr Ala Tyr Thr Leu Ala Glu Leu Asp Tyr Phe Ile           
                85                  90                  95                

ggc gat gcc gaa ccg cgt ctg gtg gtc gtt gct ccc gca gct cga ggt        336
Gly Asp Ala Glu Pro Arg Leu Val Val Val Ala Pro Ala Ala Arg Gly           
            100                 105                 110                   

ggc gtg gag aca att gcc aag cga cac ggt gct atc gtc gaa acc ctc        384
Gly Val Glu Thr Ile Ala Lys Arg His Gly Ala Ile Val Glu Thr Leu           
        115                 120                 125                       

gac gcc gat gga cga ggc tcc ttg ctg gac ctt gct aga gat gag cct        432
Asp Ala Asp Gly Arg Gly Ser Leu Leu Asp Leu Ala Arg Asp Glu Pro           
    130                 135                 140                           

gcc gac ttt gtc gat gct tcg cga tct gcc gac gat ctg gct gct att        480
Ala Asp Phe Val Asp Ala Ser Arg Ser Ala Asp Asp Leu Ala Ala Ile           
145                 150                 155                 160           

ctc tac act tcc ggt aca acc gga cga tcg aag ggt gcc atg ctt act        528
Leu Tyr Thr Ser Gly Thr Thr Gly Arg Ser Lys Gly Ala Met Leu Thr           
                165                 170                 175               

cat ggc aat ctg ctc tcc aac gct ctc acc ttg cga gac tat tgg aga        576
His Gly Asn Leu Leu Ser Asn Ala Leu Thr Leu Arg Asp Tyr Trp Arg           
            180                 185                 190                   

gtt acc gca gac gat cga ctc atc cat gcc ttg cca atc ttt cac act        624
Val Thr Ala Asp Asp Arg Leu Ile His Ala Leu Pro Ile Phe His Thr           
        195                 200                 205                       

cat ggt ctg ttc gtt gct acg aac gtc aca ctg ctt gca gga gcc tcg        672
His Gly Leu Phe Val Ala Thr Asn Val Thr Leu Leu Ala Gly Ala Ser           
    210                 215                 220                           

atg ttt ctg ctc tcc aag ttc gat gcc gac gag gtc gtt tct ctc atg        720
Met Phe Leu Leu Ser Lys Phe Asp Ala Asp Glu Val Val Ser Leu Met           
225                 230                 235                 240           

cca cag gcc acc atg ctt atg ggc gtg ccc aca ttc tac gtt cga ttg        768
Pro Gln Ala Thr Met Leu Met Gly Val Pro Thr Phe Tyr Val Arg Leu           
                245                 250                 255               

ctg cag agt cct cga ctc gag aag ggt gct gtg gcc agc atc aga ctg        816
Leu Gln Ser Pro Arg Leu Glu Lys Gly Ala Val Ala Ser Ile Arg Leu           
            260                 265                 270                   

ttc att tct gga tca gct ccc ttg ctt gcc gaa acc cac gcc gag ttt        864
Phe Ile Ser Gly Ser Ala Pro Leu Leu Ala Glu Thr His Ala Glu Phe           
        275                 280                 285                       

cat gct cgt act ggt cac gcc att ctc gag cga tac ggc atg acg gaa        912
His Ala Arg Thr Gly His Ala Ile Leu Glu Arg Tyr Gly Met Thr Glu           
    290                 295                 300                           

acc aac atg aat act tcc aac ccc tac gag ggc aag cgt att gcc gga        960
Thr Asn Met Asn Thr Ser Asn Pro Tyr Glu Gly Lys Arg Ile Ala Gly           
305                 310                 315                 320           

acc gtt ggt ttt cct ctg ccc gac gtc act gtg cga gtc acc gat ccc       1008
Thr Val Gly Phe Pro Leu Pro Asp Val Thr Val Arg Val Thr Asp Pro           
                325                 330                 335               

gcc acc ggt ctc gtt ctt cca cct gaa gag act ggc atg atc gag atc       1056
Ala Thr Gly Leu Val Leu Pro Pro Glu Glu Thr Gly Met Ile Glu Ile           
            340                 345                 350                   

aag gga ccc aac gtc ttc aag ggc tat tgg cga atg ccc gaa aag acc       1104
Lys Gly Pro Asn Val Phe Lys Gly Tyr Trp Arg Met Pro Glu Lys Thr           
        355                 360                 365                       

gct gcc gag ttt acc gca gac ggt ttc ttt atc tct gga gat ctc ggc       1152
Ala Ala Glu Phe Thr Ala Asp Gly Phe Phe Ile Ser Gly Asp Leu Gly           
    370                 375                 380                           

aag atc gac cga gaa ggt tac gtt cac att gtg gga cga ggc aag gac       1200
Lys Ile Asp Arg Glu Gly Tyr Val His Ile Val Gly Arg Gly Lys Asp           
385                 390                 395                 400           

ctg gtc att tcc ggt ggc tac aac atc tat ccc aaa gag gtc gaa ggc       1248
Leu Val Ile Ser Gly Gly Tyr Asn Ile Tyr Pro Lys Glu Val Glu Gly           
                405                 410                 415               

gag atc gac cag atc gag ggt gtg gtc gag tct gct gtc att ggt gtt       1296
Glu Ile Asp Gln Ile Glu Gly Val Val Glu Ser Ala Val Ile Gly Val           
            420                 425                 430                   

cct cat ccc gat ttc gga gaa ggt gtc acc gct gtt gtc gtg tgc aaa       1344
Pro His Pro Asp Phe Gly Glu Gly Val Thr Ala Val Val Val Cys Lys           
        435                 440                 445                       

cct ggt gcc gtt ctc gac gaa aag acc atc gtg tct gct ctg cag gac       1392
Pro Gly Ala Val Leu Asp Glu Lys Thr Ile Val Ser Ala Leu Gln Asp           
    450                 455                 460                           

cgt ctt gcc cga tac aag caa ccc aag cgg att atc ttt gcc gac gat       1440
Arg Leu Ala Arg Tyr Lys Gln Pro Lys Arg Ile Ile Phe Ala Asp Asp           
465                 470                 475                 480           

ctg cct cga aac act atg gga aag gtt cag aag aac att ctt cga cag       1488
Leu Pro Arg Asn Thr Met Gly Lys Val Gln Lys Asn Ile Leu Arg Gln           
                485                 490                 495               

caa tac gcc gat ctc tac acc aga cga taa                               1518
Gln Tyr Ala Asp Leu Tyr Thr Arg Arg                                       
            500                 505                                       


<210>  42
<211>  505
<212>  PRT
<213>  Rhizobium leguminosarum bv. viciae 3841

<400>  42

Met Val Ser Asn His Leu Phe Asp Ala Met Arg Ala Ala Ala Pro Gly 
1               5                   10                  15      


Asp Ala Pro Phe Ile Arg Ile Asp Asn Ala Arg Thr Trp Thr Tyr Asp 
            20                  25                  30          


Asp Ala Ile Ala Leu Ser Gly Arg Ile Ala Gly Ala Met Asp Ala Leu 
        35                  40                  45              


Gly Ile Arg Pro Gly Asp Arg Val Ala Val Gln Val Glu Lys Ser Ala 
    50                  55                  60                  


Glu Ala Leu Ile Leu Tyr Leu Ala Cys Leu Arg Thr Gly Ala Val Tyr 
65                  70                  75                  80  


Leu Pro Leu Asn Thr Ala Tyr Thr Leu Ala Glu Leu Asp Tyr Phe Ile 
                85                  90                  95      


Gly Asp Ala Glu Pro Arg Leu Val Val Val Ala Pro Ala Ala Arg Gly 
            100                 105                 110         


Gly Val Glu Thr Ile Ala Lys Arg His Gly Ala Ile Val Glu Thr Leu 
        115                 120                 125             


Asp Ala Asp Gly Arg Gly Ser Leu Leu Asp Leu Ala Arg Asp Glu Pro 
    130                 135                 140                 


Ala Asp Phe Val Asp Ala Ser Arg Ser Ala Asp Asp Leu Ala Ala Ile 
145                 150                 155                 160 


Leu Tyr Thr Ser Gly Thr Thr Gly Arg Ser Lys Gly Ala Met Leu Thr 
                165                 170                 175     


His Gly Asn Leu Leu Ser Asn Ala Leu Thr Leu Arg Asp Tyr Trp Arg 
            180                 185                 190         


Val Thr Ala Asp Asp Arg Leu Ile His Ala Leu Pro Ile Phe His Thr 
        195                 200                 205             


His Gly Leu Phe Val Ala Thr Asn Val Thr Leu Leu Ala Gly Ala Ser 
    210                 215                 220                 


Met Phe Leu Leu Ser Lys Phe Asp Ala Asp Glu Val Val Ser Leu Met 
225                 230                 235                 240 


Pro Gln Ala Thr Met Leu Met Gly Val Pro Thr Phe Tyr Val Arg Leu 
                245                 250                 255     


Leu Gln Ser Pro Arg Leu Glu Lys Gly Ala Val Ala Ser Ile Arg Leu 
            260                 265                 270         


Phe Ile Ser Gly Ser Ala Pro Leu Leu Ala Glu Thr His Ala Glu Phe 
        275                 280                 285             


His Ala Arg Thr Gly His Ala Ile Leu Glu Arg Tyr Gly Met Thr Glu 
    290                 295                 300                 


Thr Asn Met Asn Thr Ser Asn Pro Tyr Glu Gly Lys Arg Ile Ala Gly 
305                 310                 315                 320 


Thr Val Gly Phe Pro Leu Pro Asp Val Thr Val Arg Val Thr Asp Pro 
                325                 330                 335     


Ala Thr Gly Leu Val Leu Pro Pro Glu Glu Thr Gly Met Ile Glu Ile 
            340                 345                 350         


Lys Gly Pro Asn Val Phe Lys Gly Tyr Trp Arg Met Pro Glu Lys Thr 
        355                 360                 365             


Ala Ala Glu Phe Thr Ala Asp Gly Phe Phe Ile Ser Gly Asp Leu Gly 
    370                 375                 380                 


Lys Ile Asp Arg Glu Gly Tyr Val His Ile Val Gly Arg Gly Lys Asp 
385                 390                 395                 400 


Leu Val Ile Ser Gly Gly Tyr Asn Ile Tyr Pro Lys Glu Val Glu Gly 
                405                 410                 415     


Glu Ile Asp Gln Ile Glu Gly Val Val Glu Ser Ala Val Ile Gly Val 
            420                 425                 430         


Pro His Pro Asp Phe Gly Glu Gly Val Thr Ala Val Val Val Cys Lys 
        435                 440                 445             


Pro Gly Ala Val Leu Asp Glu Lys Thr Ile Val Ser Ala Leu Gln Asp 
    450                 455                 460                 


Arg Leu Ala Arg Tyr Lys Gln Pro Lys Arg Ile Ile Phe Ala Asp Asp 
465                 470                 475                 480 


Leu Pro Arg Asn Thr Met Gly Lys Val Gln Lys Asn Ile Leu Arg Gln 
                485                 490                 495     


Gln Tyr Ala Asp Leu Tyr Thr Arg Arg 
            500                 505 


<210>  43
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(777)

<400>  43
atg gag gtg gtg aat gaa ata gtc tca att ggg cag gaa gtt tta ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aaa gtt gat tat gcc caa ctc tgg agt gat gcc agt cac tgt gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctt tac ttg tcc atc gca ttt gtc atc ttg aag ttc act ctt ggc ccc        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctt ggt cca aaa ggt cag tct cgt atg aag ttt gtt ttc acc aat tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctt ctc atg tcc att tat tcg ttg gga tca ttc ctc tca atg gca        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tat gcc atg tac acc atc ggt gtt atg tct gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttt gac aac aac gtc ttc agg atc acc acg cag ttg ttc tat ttg agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctg gag tat att gac tcc ttc tat ttg cca ctg atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg caa ttc ttc cat cat ttg ggg gca ccg atg gat        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tat aat tac cga aat gaa gct gtt tgg att ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ttg aat ggt ttc atc cac tgg atc atg tac ggt tat tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

aga ttg atc aag ctg aag ttc ccc atg cca aaa tcc ctg att aca tca        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att caa ttc aat gtt ggt ttc tac att gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

agg aac att ccc tgt tat cgc caa gat ggg atg agg atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttc ttc aat tac ttt tat gtt ggc aca gtc ttg tgt ttg ttc ttg aat        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tat gtg caa acg tat atc gtc agg aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  44
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  44

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  45
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(777)

<400>  45
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  46
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  46

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  47
<211>  792
<212>  DNA
<213>  Eutreptiella sp. CCMP389


<220>
<221>  CDS
<222>  (1)..(792)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(792)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(792)

<400>  47
atg gct gcg gtg ata gag gtc gcc aac gag ttt gta gcc atc acg gca         48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala           
1               5                   10                  15                

gaa acg ctc ccc aaa gtt gac tat caa cga cta tgg cga gac att tac         96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr           
            20                  25                  30                    

agt tgt gag cta ctg tat ttc tcc att gcc ttc gtg atc ttg aag ttt        144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe           
        35                  40                  45                        

acg ttg ggc gag ttg agc gac agc gga aaa aag att ttg aga gtg ttg        192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu           
    50                  55                  60                            

ttc aag tgg tac aat ctc ttc atg tcc gtg ttc tcc ttg gtg tct ttc        240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe           
65                  70                  75                  80            

ctt tgc atg ggc tat gcc att tat acc gtg ggc cta tac tct aac gaa        288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu           
                85                  90                  95                

tgc gac agg gct ttc gac aac tcg ttg ttc cgc ttt gca aca aag gtg        336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val           
            100                 105                 110                   

ttc tac tac agt aag ttt ttg gag tac atc gac tct ttt tat ctt ccg        384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro           
        115                 120                 125                       

ctc atg gcc aag ccg ctg tct ttc ctg caa ttc ttc cat cac ttg gga        432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly           
    130                 135                 140                           

gcc ccc atg gac atg tgg ctc ttt gtc caa tat tct ggg gaa tct att        480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile           
145                 150                 155                 160           

tgg atc ttt gtg ttt ttg aat ggg ttc att cac ttt gtt atg tac ggg        528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly           
                165                 170                 175               

tac tac tgg act cgg ctg atg aag ttc aat ttc cca atg ccc aag cag        576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln           
            180                 185                 190                   

ttg att acc gcg atg cag atc acg cag ttc aac gtt ggt ttc tac ctc        624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu           
        195                 200                 205                       

gtg tgg tgg tac aaa gat att ccc tgc tac cga aag gat ccc atg cga        672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg           
    210                 215                 220                           

atg ttg gcc tgg atc ttc aat tac tgg tat gtt ggg act gtc ttg ctg        720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu           
225                 230                 235                 240           

ctg ttc att aat ttc ttc gtc aaa tcc tat gtg ttc cca aag ccg aag        768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys           
                245                 250                 255               

act gca gat aaa aag gtc caa tag                                        792
Thr Ala Asp Lys Lys Val Gln                                               
            260                                                           


<210>  48
<211>  263
<212>  PRT
<213>  Eutreptiella sp. CCMP389

<400>  48

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210>  49
<211>  792
<212>  DNA
<213>  Eutreptiella sp. CCMP389


<220>
<221>  CDS
<222>  (1)..(792)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(792)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(792)

<400>  49
atg gct gcc gtc atc gag gtg gcc aac gag ttc gtc gct atc act gcc         48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala           
1               5                   10                  15                

gag acc ctt ccc aag gtg gac tat cag cga ctc tgg cga gac atc tac         96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr           
            20                  25                  30                    

tcc tgc gag ctc ctg tac ttc tcc att gct ttc gtc atc ctc aag ttt        144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe           
        35                  40                  45                        

acc ctt ggc gag ctc tcg gat tct ggc aaa aag att ctg cga gtg ctg        192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu           
    50                  55                  60                            

ttc aag tgg tac aac ctc ttc atg tcc gtc ttt tcg ctg gtg tcc ttc        240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe           
65                  70                  75                  80            

ctc tgt atg ggt tac gcc atc tac acc gtt gga ctg tac tcc aac gaa        288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu           
                85                  90                  95                

tgc gac aga gct ttc gac aac agc ttg ttc cga ttt gcc acc aag gtc        336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val           
            100                 105                 110                   

ttc tac tat tcc aag ttt ctg gag tac atc gac tct ttc tac ctt ccc        384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro           
        115                 120                 125                       

ctc atg gcc aag cct ctg tcc ttt ctg cag ttc ttt cat cac ttg gga        432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly           
    130                 135                 140                           

gct cct atg gac atg tgg ctc ttc gtg cag tac tct ggc gaa tcc att        480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile           
145                 150                 155                 160           

tgg atc ttt gtg ttc ctg aac gga ttc att cac ttt gtc atg tac ggc        528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly           
                165                 170                 175               

tac tat tgg aca cgg ctg atg aag ttc aac ttt ccc atg ccc aag cag        576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln           
            180                 185                 190                   

ctc att acc gca atg cag atc acc cag ttc aac gtt ggc ttc tac ctc        624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu           
        195                 200                 205                       

gtg tgg tgg tac aag gac att ccc tgt tac cga aag gat ccc atg cga        672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg           
    210                 215                 220                           

atg ctg gcc tgg atc ttc aac tac tgg tac gtc ggt acc gtt ctt ctg        720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu           
225                 230                 235                 240           

ctc ttc atc aac ttc ttt gtc aag tcc tac gtg ttt ccc aag cct aag        768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys           
                245                 250                 255               

act gcc gac aaa aag gtc cag tag                                        792
Thr Ala Asp Lys Lys Val Gln                                               
            260                                                           


<210>  50
<211>  263
<212>  PRT
<213>  Eutreptiella sp. CCMP389

<400>  50

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210>  51
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 2008-0254522-A1
<311>  2008-04-15
<312>  2008-10-16
<313>  (1)..(774)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2008/128241
<311>  2008-04-16
<312>  2008-10-23
<313>  (1)..(774)

<400>  51
atg gaa gca gcc aaa gaa ttg gtt tcc atc gtc caa gag gag ctc ccc         48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro           
1               5                   10                  15                

aag gtg gac tat gcc cag ctt tgg cag gat gcc agc agc tgt gag gtc         96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val           
            20                  25                  30                    

ctt tac ctc tcg gtg gca ttc gtg gcg atc aag ttc atg ctg cgc cca        144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro           
        35                  40                  45                        

ctg gac ctg aag cgc cag gcc acc ttg aag aag ctg ttc aca gca tac        192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr           
    50                  55                  60                            

aac ttc ctc atg tcg atc tat tcc ttt ggc tcc ttc ctg gcc atg gcc        240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala           
65                  70                  75                  80            

tat gcc cta tca gta act ggc atc ctc tcc ggc gac tgt gag acg gcg        288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala           
                85                  90                  95                

ttc aac aac gat gtg ttc agg atc aca act cag ctg ttc tac ctc agc        336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc gta gag tac atc gac tcc ttc tac ctt ccc ctt atg gac aag        384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys           
        115                 120                 125                       

cca ctg tcg ttc ctt cag ttc ttc cat cat ttg ggg gcc ccc att gac        432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp           
    130                 135                 140                           

atg tgg cta ttc tac aaa tac cgc aac gaa gga gtc tgg atc ttt gtc        480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ttg aat ggg ttc att cac tgg atc atg tac ggt tac tat tgg acg        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cgg ctc atc aag ctg aac ttc ccc atg ccc aag aac ctg atc acc tcc        576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc atc cag ttc aat gtc ggg ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgc aat gtg cca tgc tac cgc cag gat ggg atg cgc atg ttt gcc tgg        672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp           
    210                 215                 220                           

atc ttc aac tac tgg tat gtc ggg acg gtc ttg ctg ctg ttc ctc aac        720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn           
225                 230                 235                 240           

ttt tac gtg cag acg tac atc cgg aag ccg agg aag aac cga ggg aag        768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys           
                245                 250                 255               

aag gag                                                                774
Lys Glu                                                                   
                                                                          


<210>  52
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  52

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  53
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 2008-0254522-A1
<311>  2008-04-15
<312>  2008-10-16
<313>  (1)..(774)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2008/128241
<311>  2008-04-16
<312>  2008-10-23
<313>  (1)..(774)

<400>  53
atg gag gct gcc aag gag ctg gtc tcc atc gtc cag gag gaa ctt ccc         48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro           
1               5                   10                  15                

aag gtg gac tac gcc cag ctc tgg cag gac gcc tcc tct tgc gag gtt         96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcg gtc gct ttc gtg gcc atc aag ttc atg ctt cga cct        144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro           
        35                  40                  45                        

ctg gac ctc aag cga caa gcc acc ctc aaa aag ctg ttc acc gca tac        192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr           
    50                  55                  60                            

aac ttt ctc atg tcc atc tac tcg ttc ggc tcc ttc ctg gcg atg gcc        240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala           
65                  70                  75                  80            

tac gct ctc tct gtc act ggt att ctt tcc ggc gat tgt gag act gcc        288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala           
                85                  90                  95                

ttc aac aat gac gtg ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc gtc gag tac atc gac tcc ttc tac ctt ccc ctc atg gac aag        384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys           
        115                 120                 125                       

ccc ttg tcg ttt ctg cag ttc ttt cac cat ctc gga gct ccc atc gac        432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aag tat cga aac gaa ggc gtc tgg atc ttt gtt        480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggt tac tat tgg acg        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctc atc aag ctg aac ttc cct atg ccc aag aac ctc att acc tcc        576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser           
            180                 185                 190                   

atg caa att atc cag ttc aac gtc gga ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cga aac gtg ccc tgc tac cgg cag gac ggt atg cga atg ttt gcc tgg        672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp           
    210                 215                 220                           

atc ttc aac tac tgg tat gtc ggc acg gtg ctg ctt ctg ttc ctc aac        720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtc cag acc tac att cgg aag cct cga aag aac cga ggc aaa        768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys           
                245                 250                 255               

aag gag                                                                774
Lys Glu                                                                   
                                                                          


<210>  54
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  54

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  55
<211>  1271
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (4)..(1269)
<223>  delta-8 desaturase ("Eg5" or "EgD8")

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2006/012325 and WO 2006/012326
<311>  2005-06-24
<312>  2006-02-02
<313>  (1)..(1271)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,256,033
<311>  2005-06-24
<312>  2007-08-14
<313>  (1)..(1271)

<400>  55
gaa atg aag tca aag cgc caa gcg ctt ccc ctt aca att gat gga aca         48
    Met Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr           
    1               5                   10                  15            

aca tat gat gtg tct gcc tgg gtc aat ttc cac cct ggt ggt gcg gaa         96
Thr Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu           
                20                  25                  30                

att ata gag aat tac caa gga agg gat gcc act gat gcc ttc atg gtt        144
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val           
            35                  40                  45                    

atg cac tct caa gaa gcc ttc gac aag ctc aag cgc atg ccc aaa atc        192
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile           
        50                  55                  60                        

aat ccc agt tct gag ttg cca ccc cag gct gca gtg aat gaa gct caa        240
Asn Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln           
    65                  70                  75                            

gag gat ttc cgg aag ctc cga gaa gag ttg atc gca act ggc atg ttt        288
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe           
80                  85                  90                  95            

gat gcc tcc ccc ctc tgg tac tca tac aaa atc agc acc aca ctg ggc        336
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly           
                100                 105                 110               

ctt gga gtg ctg ggt tat ttc ctg atg gtt cag tat cag atg tat ttc        384
Leu Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe           
            115                 120                 125                   

att ggg gca gtg ttg ctt ggg atg cac tat caa cag atg ggc tgg ctt        432
Ile Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu           
        130                 135                 140                       

tct cat gac att tgc cac cac cag act ttc aag aac cgg aac tgg aac        480
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn           
    145                 150                 155                           

aac ctc gtg gga ctg gta ttt ggc aat ggt ctg caa ggt ttt tcc gtg        528
Asn Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val           
160                 165                 170                 175           

aca tgg tgg aag gac aga cac aat gca cat cat tcg gca acc aat gtt        576
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val           
                180                 185                 190               

caa ggg cac gac cct gat att gac aac ctc ccc ctc tta gcc tgg tct        624
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser           
            195                 200                 205                   

gag gat gac gtc aca cgg gcg tca ccg att tcc cgc aag ctc att cag        672
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln           
        210                 215                 220                       

ttc cag cag tac tat ttc ttg gtc atc tgt atc ttg ttg cgg ttc att        720
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile           
    225                 230                 235                           

tgg tgt ttc cag agc gtg ttg acc gtg cgc agt ttg aag gac aga gat        768
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp           
240                 245                 250                 255           

aac caa ttc tat cgc tct cag tat aag aag gag gcc att ggc ctc gcc        816
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala           
                260                 265                 270               

ctg cac tgg acc ttg aag acc ctg ttc cac tta ttc ttt atg ccc agc        864
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser           
            275                 280                 285                   

atc ctc aca tcg ctg ttg gtg ttt ttc gtt tcg gag ctg gtt ggc ggc        912
Ile Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly           
        290                 295                 300                       

ttc ggc att gcg atc gtg gtg ttc atg aac cac tac cca ctg gag aag        960
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys           
    305                 310                 315                           

atc ggg gac tca gtc tgg gat ggc cat gga ttc tcg gtt ggc cag atc       1008
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile           
320                 325                 330                 335           

cat gag acc atg aac att cgg cga ggg att atc aca gat tgg ttt ttc       1056
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe           
                340                 345                 350               

gga ggc ttg aat tac cag att gag cac cat ttg tgg ccg acc ctc cct       1104
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro           
            355                 360                 365                   

cgc cac aac ctg aca gcg gtt agc tac cag gtg gaa cag ctg tgc cag       1152
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln           
        370                 375                 380                       

aag cac aac ctg ccg tat cgg aac ccg ctg ccc cat gaa ggg ttg gtc       1200
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val           
    385                 390                 395                           

atc ctg ctg cgc tat ctg gcg gtg ttc gcc cgg atg gcg gag aag caa       1248
Ile Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln           
400                 405                 410                 415           

ccc gcg ggg aag gct cta taa gg                                        1271
Pro Ala Gly Lys Ala Leu                                                   
                420                                                       


<210>  56
<211>  421
<212>  PRT
<213>  Euglena gracilis

<400>  56

Met Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr Thr 
1               5                   10                  15      


Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu Ile 
            20                  25                  30          


Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val Met 
        35                  40                  45              


His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn 
    50                  55                  60                  


Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu 
65                  70                  75                  80  


Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp 
                85                  90                  95      


Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly Leu 
            100                 105                 110         


Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe Ile 
        115                 120                 125             


Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu Ser 
    130                 135                 140                 


His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn 
145                 150                 155                 160 


Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val Thr 
                165                 170                 175     


Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val Gln 
            180                 185                 190         


Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu 
        195                 200                 205             


Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe 
    210                 215                 220                 


Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp 
225                 230                 235                 240 


Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp Asn 
                245                 250                 255     


Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu 
            260                 265                 270         


His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser Ile 
        275                 280                 285             


Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly Phe 
    290                 295                 300                 


Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile 
305                 310                 315                 320 


Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His 
                325                 330                 335     


Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly 
            340                 345                 350         


Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg 
        355                 360                 365             


His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys 
    370                 375                 380                 


His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val Ile 
385                 390                 395                 400 


Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln Pro 
                405                 410                 415     


Ala Gly Lys Ala Leu 
            420     


<210>  57
<211>  1272
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (2)..(1270)
<223>  synthetic delta-8 desaturase (codon-optimized for Yarrowia 
       lipolytica) ("D8SF" or "EgD8S")

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2006/012325 and WO 2006/012326
<311>  2005-06-24
<312>  2006-02-02
<313>  (1)..(1272)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,256,033
<311>  2005-06-24
<312>  2007-08-14
<313>  (1)..(1272)

<400>  57
c atg gtg aag tcc aag cga cag gct ctg ccc ctc acc atc gac gga act       49
  Met Val Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr         
  1               5                   10                  15              

acc tac gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa         97
Thr Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu           
            20                  25                  30                    

atc att gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt        145
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val           
        35                  40                  45                        

atg cac tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc        193
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile           
    50                  55                  60                            

aac ccc tcc tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag        241
Asn Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln           
65                  70                  75                  80            

gag gat ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt        289
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe           
                85                  90                  95                

gac gcc tct ccc ctc tgg tac tcg tac aag atc tcc acc acc ctg ggt        337
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly           
            100                 105                 110                   

ctt ggc gtg ctt gga tac ttc ctg atg gtc cag tac cag atg tac ttc        385
Leu Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe           
        115                 120                 125                       

att ggt gct gtg ctg ctc ggt atg cac tac cag caa atg gga tgg ctg        433
Ile Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu           
    130                 135                 140                           

tct cat gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat        481
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn           
145                 150                 155                 160           

aac ctc gtg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg        529
Asn Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val           
                165                 170                 175               

acc tgg tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt        577
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val           
            180                 185                 190                   

cag ggt cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc        625
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser           
        195                 200                 205                       

gag gac gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag        673
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln           
    210                 215                 220                           

ttc caa cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc        721
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile           
225                 230                 235                 240           

tgg tgt ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac        769
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp           
                245                 250                 255               

aac cag ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct        817
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala           
            260                 265                 270                   

ctg cac tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc        865
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser           
        275                 280                 285                       

atc ctg acc tcg ctc ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc        913
Ile Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly           
    290                 295                 300                           

ttc gga att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag        961
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys           
305                 310                 315                 320           

atc ggt gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc       1009
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile           
                325                 330                 335               

cat gag acc atg aac att cga cga ggc atc att act gac tgg ttc ttt       1057
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe           
            340                 345                 350                   

gga ggc ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct       1105
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro           
        355                 360                 365                       

cga cac aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag       1153
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln           
    370                 375                 380                           

aag cac aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc       1201
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val           
385                 390                 395                 400           

atc ctg ctc cga tac ctg gcc gtg ttc gct cga atg gcc gag aag cag       1249
Ile Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln           
                405                 410                 415               

ccc gct ggc aag gct ctc taa gc                                        1272
Pro Ala Gly Lys Ala Leu                                                   
            420                                                           


<210>  58
<211>  422
<212>  PRT
<213>  Euglena gracilis

<400>  58

Met Val Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr 
1               5                   10                  15      


Thr Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe 
        115                 120                 125             


Ile Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Ala Gly Lys Ala Leu 
            420         


<210>  59
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-23 delta-8 desaturase (also "EgD8M")


<220>
<221>  CDS
<222>  (2)..(1270)

<300>
<302>  MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS
        AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/073271
<311>  2007-12-05
<312>  2008-06-19
<313>  (1)..(1272)

<300>
<302>  MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS
        AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US 2008-0138868-A1
<311>  2006-12-07
<312>  2008-06-12
<313>  (1)..(1272)

<400>  59
c atg gtg aag gct tct cga cag gct ctg ccc ctc gtc atc gac gga aag       49
  Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys         
  1               5                   10                  15              

gtg tac gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa         97
Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu           
            20                  25                  30                    

atc att gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt        145
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val           
        35                  40                  45                        

atg cac tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc        193
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile           
    50                  55                  60                            

aac cag gct tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag        241
Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln           
65                  70                  75                  80            

gag gat ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt        289
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe           
                85                  90                  95                

gac gcc tct ccc ctc tgg tac tcg tac aag atc ttg acc acc ctg ggt        337
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly           
            100                 105                 110                   

ctt ggc gtg ctt gcc ttc ttc atg ctg gtc cag tac cac ctg tac ttc        385
Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe           
        115                 120                 125                       

att ggt gct ctc gtg ctc ggt atg cac tac cag caa atg gga tgg ctg        433
Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu           
    130                 135                 140                           

tct cat gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat        481
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn           
145                 150                 155                 160           

aac gtc ctg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg        529
Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val           
                165                 170                 175               

acc tgg tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt        577
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val           
            180                 185                 190                   

cag ggt cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc        625
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser           
        195                 200                 205                       

gag gac gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag        673
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln           
    210                 215                 220                           

ttc caa cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc        721
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile           
225                 230                 235                 240           

tgg tgt ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac        769
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp           
                245                 250                 255               

aac cag ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct        817
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala           
            260                 265                 270                   

ctg cac tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc        865
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser           
        275                 280                 285                       

atc ctg acc tcg atg ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc        913
Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly           
    290                 295                 300                           

ttc gga att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag        961
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys           
305                 310                 315                 320           

atc ggt gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc       1009
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile           
                325                 330                 335               

cat gag acc atg aac att cga cga ggc atc att act gac tgg ttc ttt       1057
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe           
            340                 345                 350                   

gga ggc ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct       1105
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro           
        355                 360                 365                       

cga cac aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag       1153
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln           
    370                 375                 380                           

aag cac aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc       1201
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val           
385                 390                 395                 400           

atc ctg ctc cga tac ctg tcc cag ttc gct cga atg gcc gag aag cag       1249
Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln           
                405                 410                 415               

ccc ggt gcc aag gct cag taa gc                                        1272
Pro Gly Ala Lys Ala Gln                                                   
            420                                                           


<210>  60
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  60

Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys 
1               5                   10                  15      


Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe 
        115                 120                 125             


Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Gly Ala Lys Ala Gln 
            420         


<210>  61
<211>  1260
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  delta-8 desaturase

<300>
<302>  DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  U.S. 2008-0254521-A1
<311>  2008-04-09
<312>  2008-10-16
<313>  (1)..(1260)

<300>
<302>  DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  WO 2008/124194
<311>  2008-04-10
<312>  2008-10-16
<313>  (1)..(1260)

<400>  61
atg gtg aaa agg cca gca ctt ccg ctg acc gtt gat ggt gtc acc tat         48
Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr           
1               5                   10                  15                

gat gtg tct gcc tgg ttg aac cat cat cca ggg ggt gct gac atc att         96
Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile           
            20                  25                  30                    

gag aac tac cgc ggt cgt gat gcc act gat gtc ttt atg gtt atg cac        144
Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His           
        35                  40                  45                        

tct gaa aat gct gtg agt aaa cta aga agg atg cct atc atg gaa cca        192
Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro           
    50                  55                  60                            

tca tct cca ctg acg cct acg cca ccg aaa ccc aac tca gac gaa ccg        240
Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro           
65                  70                  75                  80            

cag gag gat ttc cgc aag ctc cga gat gag ctc atc gca gca gga atg        288
Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met           
                85                  90                  95                

ttc gac gca tca ccg atg tgg tac gca tat aag acg ctc agt acg ctg        336
Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu           
            100                 105                 110                   

ggc ctc ggg gtc ctc gcg gtg cta ttg atg acc cag tgg cac tgg tac        384
Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr           
        115                 120                 125                       

ctc gtc ggg gca atc gtg ttg ggc att cac ttc caa caa atg ggt tgg        432
Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp           
    130                 135                 140                           

ttg tcg cac gat atc tgc cac cat cag ctg ttc aag gac cga tcg atc        480
Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile           
145                 150                 155                 160           

aac aac gcc atc ggc ttg ctt ttc ggg aac gtc ttg caa ggg ttc tct        528
Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser           
                165                 170                 175               

gtg acc tgg tgg aag gac agg cac aat gca cac cac tcc gcc acc aac        576
Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn           
            180                 185                 190                   

gtg caa ggc cac gac ccc gac att gac aac ctg ccg ctg ctg gca tgg        624
Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp           
        195                 200                 205                       

tcc aag gag gac gtg gag agg gcc ggc ccg ttc tca cgg cgg atg atc        672
Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile           
    210                 215                 220                           

aag tac cag caa tac tac ttc ttc ttc atc tgt gcc ctc ctg agg ttc        720
Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe           
225                 230                 235                 240           

atc tgg tgc ttc cag agc atc cac aca gcc acg ggc ctg aag gat cgc        768
Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg           
                245                 250                 255               

agc aac cag tac tac cgc agg cag tac gag aaa gag agc gtg ggc ctg        816
Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu           
            260                 265                 270                   

gcc ctc cac tgg ggc ctg aag gcg ttg ttc tac tac ttt tat atg cca        864
Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro           
        275                 280                 285                       

agc ttc ttg acc gga ctc atg gtg ttt ttc gtg tcc gag ttg ctt ggg        912
Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly           
    290                 295                 300                           

ggc ttc ggc atc gcc atc gtg gtg ttc atg aac cac tac ccc ctg gag        960
Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu           
305                 310                 315                 320           

aag atc cag gac tcg gtg tgg gac ggc cac ggc ttt tgc gcc ggc cag       1008
Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln           
                325                 330                 335               

att cac gaa acg atg aac gtc cag cgg gga ctc gtc acg gac tgg ttc       1056
Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe           
            340                 345                 350                   

ttc ggt ggg ctg aat tac caa atc gag cac cac ctg tgg ccg acg ctg       1104
Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu           
        355                 360                 365                       

ccc cgg cac aac ctg acg gcg gcc agc atc aaa gtg gag cag ttg tgc       1152
Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys           
    370                 375                 380                           

aag aag cac aac ttg ccg tat cgc agc ccc cca atg ctg gag ggg gtg       1200
Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val           
385                 390                 395                 400           

ggc atc ctg atc agc tac ctg ggc acc ttt gcc cgc atg gtg gca aag       1248
Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys           
                405                 410                 415               

gcc gac aag gcg                                                       1260
Ala Asp Lys Ala                                                           
            420                                                           


<210>  62
<211>  420
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  62

Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr 
1               5                   10                  15      


Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile 
            20                  25                  30          


Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His 
        35                  40                  45              


Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro 
    50                  55                  60                  


Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro 
65                  70                  75                  80  


Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met 
                85                  90                  95      


Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu 
            100                 105                 110         


Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr 
        115                 120                 125             


Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp 
    130                 135                 140                 


Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile 
145                 150                 155                 160 


Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser 
                165                 170                 175     


Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn 
            180                 185                 190         


Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp 
        195                 200                 205             


Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile 
    210                 215                 220                 


Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe 
225                 230                 235                 240 


Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg 
                245                 250                 255     


Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu 
            260                 265                 270         


Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro 
        275                 280                 285             


Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly 
    290                 295                 300                 


Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu 
305                 310                 315                 320 


Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln 
                325                 330                 335     


Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe 
            340                 345                 350         


Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu 
        355                 360                 365             


Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys 
    370                 375                 380                 


Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val 
385                 390                 395                 400 


Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys 
                405                 410                 415     


Ala Asp Lys Ala 
            420 


<210>  63
<211>  1260
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  synthetic delta-8 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  U.S. 2008-0254521-A1
<311>  2008-04-09
<312>  2008-10-16
<313>  (1)..(1260)

<300>
<302>  DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  WO 2008/124194
<311>  2008-04-10
<312>  2008-10-16
<313>  (1)..(1260)

<400>  63
atg gtc aag cga ccc gct ctg cct ctc acc gtg gac ggt gtc acc tac         48
Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr           
1               5                   10                  15                

gac gtt tct gcc tgg ctc aac cac cat ccc gga ggt gcc gac att atc         96
Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile           
            20                  25                  30                    

gag aac tac cga ggt cgg gat gct acc gac gtc ttc atg gtt atg cac        144
Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His           
        35                  40                  45                        

tcc gag aac gcc gtg tcc aaa ctc aga cga atg ccc atc atg gaa cct        192
Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro           
    50                  55                  60                            

tcc tct ccc ctg act cca aca cct ccc aag cca aac tcc gac gaa cct        240
Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro           
65                  70                  75                  80            

cag gag gat ttc cga aag ctg cga gac gag ctc att gct gca ggc atg        288
Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met           
                85                  90                  95                

ttc gat gcc tct ccc atg tgg tac gct tac aag acc ctg tcg act ctc        336
Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu           
            100                 105                 110                   

gga ctg ggt gtc ctt gcc gtg ctg ttg atg acc cag tgg cac tgg tac        384
Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr           
        115                 120                 125                       

ctg gtt ggt gct atc gtc ctc ggc att cac ttt caa cag atg gga tgg        432
Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp           
    130                 135                 140                           

ctc tcg cac gac att tgc cat cac cag ctg ttc aag gac cga tcc atc        480
Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile           
145                 150                 155                 160           

aac aat gcc att ggc ctg ctc ttc gga aac gtg ctt cag ggc ttt tct        528
Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser           
                165                 170                 175               

gtc act tgg tgg aag gac cga cac aac gct cat cac tcc gcc acc aac        576
Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn           
            180                 185                 190                   

gtg cag ggt cac gat ccc gac atc gac aac ctg cct ctc ctg gcg tgg        624
Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp           
        195                 200                 205                       

tcc aag gag gac gtc gag cga gct ggc ccg ttt tct cga cgg atg atc        672
Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile           
    210                 215                 220                           

aag tac caa cag tat tac ttc ttt ttc atc tgt gcc ctt ctg cga ttc        720
Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe           
225                 230                 235                 240           

atc tgg tgc ttt cag tcc att cat act gcc acg ggt ctc aag gat cga        768
Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg           
                245                 250                 255               

agc aat cag tac tat cga aga cag tac gag aag gag tcc gtc ggt ctg        816
Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu           
            260                 265                 270                   

gca ctc cac tgg ggt ctc aag gcc ttg ttc tac tat ttc tac atg ccc        864
Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro           
        275                 280                 285                       

tcg ttt ctc acc gga ctc atg gtg ttc ttt gtc tcc gag ctg ctt ggt        912
Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly           
    290                 295                 300                           

ggc ttc gga att gcc atc gtt gtc ttc atg aac cac tac cct ctg gag        960
Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu           
305                 310                 315                 320           

aag att cag gac tcc gtg tgg gat ggt cat ggc ttc tgt gct gga cag       1008
Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln           
                325                 330                 335               

att cac gag acc atg aac gtt cag cga ggc ctc gtc aca gac tgg ttt       1056
Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe           
            340                 345                 350                   

ttc ggt ggc ctc aac tac cag atc gaa cat cac ctg tgg cct act ctt       1104
Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu           
        355                 360                 365                       

ccc aga cac aac ctc acc gct gcc tcc atc aaa gtg gag cag ctg tgc       1152
Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys           
    370                 375                 380                           

aag aag cac aac ctg ccc tac cga tct cct ccc atg ctc gaa ggt gtc       1200
Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val           
385                 390                 395                 400           

ggc att ctt atc tcc tac ctg ggc acc ttc gct cga atg gtt gcc aag       1248
Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys           
                405                 410                 415               

gca gac aag gcc                                                       1260
Ala Asp Lys Ala                                                           
            420                                                           


<210>  64
<211>  420
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  64

Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr 
1               5                   10                  15      


Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile 
            20                  25                  30          


Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His 
        35                  40                  45              


Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro 
    50                  55                  60                  


Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro 
65                  70                  75                  80  


Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met 
                85                  90                  95      


Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu 
            100                 105                 110         


Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr 
        115                 120                 125             


Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp 
    130                 135                 140                 


Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile 
145                 150                 155                 160 


Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser 
                165                 170                 175     


Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn 
            180                 185                 190         


Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp 
        195                 200                 205             


Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile 
    210                 215                 220                 


Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe 
225                 230                 235                 240 


Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg 
                245                 250                 255     


Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu 
            260                 265                 270         


Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro 
        275                 280                 285             


Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly 
    290                 295                 300                 


Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu 
305                 310                 315                 320 


Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln 
                325                 330                 335     


Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe 
            340                 345                 350         


Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu 
        355                 360                 365             


Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys 
    370                 375                 380                 


Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val 
385                 390                 395                 400 


Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys 
                405                 410                 415     


Ala Asp Lys Ala 
            420 


<210>  65
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  delta-5 desaturase

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136671
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1350)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0292924-A1
<311>  2007-05-15
<312>  2007-12-20
<313>  (1)..(1350)

<400>  65
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat cca gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc        144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aat tcc aaa        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg tcc cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc caa gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc act gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag agc tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cgc cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc tga                                                               1350
Ser                                                                       
                                                                          


<210>  66
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  66

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  67
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136671
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1350)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0292924-A1
<311>  2007-05-15
<312>  2007-12-20
<313>  (1)..(1350)

<400>  67
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ccc gga ggc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct        144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc ctg tcc att cag cac gat gcc tct cat ggt        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  68
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  68

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  69
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  mutant delta-5 desaturase

<400>  69
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat cca gga tcc gat ttg att ctc gct tct ggt gcc tct gat gcc tcc        144
His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aac tcc aaa        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg tcc cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc cag gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gaa aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc acc gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag cga tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cga cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  70
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  70

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  71
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  synthetic mutant delta-5 desaturase (codon-optimized for Yarrowia
       lipolytica)

<400>  71
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ccc gga tcc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct        144
His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc ctg tcc att cag cac gat gcc tct cat ggt        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  72
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  72

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  73
<211>  1392
<212>  DNA
<213>  Peridinium sp. CCMP626


<220>
<221>  CDS
<222>  (1)..(1392)
<223>  delta-5 desaturase

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136646
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1392)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0271632-A1
<311>  2007-05-15
<312>  2007-11-22
<313>  (1)..(1392)

<400>  73
atg gct cca gat gcg gac aag ttg aga cag cgc aag gcg caa tcg att         48
Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile           
1               5                   10                  15                

caa gac acg gct gat tcg caa gct acc gaa ctc aag att ggc acc ctg         96
Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu           
            20                  25                  30                    

aag ggc ttg cag ggg aca gaa atc gtc att gat gga gac att tac gat        144
Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp           
        35                  40                  45                        

ata aaa gac ttt gat cac ccc ggt ggt gaa tcc atc atg act ttt ggg        192
Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly           
    50                  55                  60                            

gga aac gat gtc acc gcc acg tac aag atg atc cac ccc tac cac tct        240
Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser           
65                  70                  75                  80            

aag cac cat ttg gag aag atg aag aaa gtg gga cga gtt ccg gac tac        288
Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr           
                85                  90                  95                

acc tcg gaa tac aag ttt gat act ccc ttt gag cgt gaa atc aag caa        336
Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln           
            100                 105                 110                   

gag gtc ttc aag att gtg cga cga ggc cgc gag ttt gga aca cct gga        384
Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly           
        115                 120                 125                       

tac ttc ttc cgg gct ttc tgc tac att gga ctt ttc ttt tac ttg cag        432
Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln           
    130                 135                 140                           

tat ttg tgg gtc acg act ccc act acc ttt gcc ttg gcg atc ttc tat        480
Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr           
145                 150                 155                 160           

ggt gtt tcg caa gct ttc att ggt ttg aac gta caa cat gat gcc aac        528
Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn           
                165                 170                 175               

cac gga gct gcc tcc aag aag cct tgg atc aat aac ttg cta gga ttg        576
His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu           
            180                 185                 190                   

ggg gct gac ttt atc gga ggt tcc aaa tgg ttg tgg atg aac cag cac        624
Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His           
        195                 200                 205                       

tgg acg cac cac aca tac acc aac cac cat gag aag gat ccc gat gcc        672
Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala           
    210                 215                 220                           

ttg ggc gct gaa cca atg ttg ttg ttc aat gat tat ccc ttg ggt cac        720
Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His           
225                 230                 235                 240           

cca aag cgt act ttg att cac cac ttc cag gcc ttc tat tac ctt ttc        768
Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe           
                245                 250                 255               

gtc ttg gcc gga tac tgg gtc tct tcg gtc ttc aac cct caa att ttg        816
Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu           
            260                 265                 270                   

gac ttg caa cac cgc ggt gct caa gcg gtt gga atg aaa atg gag aac        864
Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn           
        275                 280                 285                       

gat tac att gcc aaa agc cga aag tat gcc atc ttc ttg cgt ctc ttg        912
Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu           
    290                 295                 300                           

tat att tac acc aac att gtc gct ccg atc caa aac caa ggc ttc tcg        960
Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser           
305                 310                 315                 320           

ttg acc gtg gtc gcc cac att ttg acc atg ggc gtc gct tcc agt ttg       1008
Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu           
                325                 330                 335               

act ttg gcg act ctt ttt gcc ttg tcg cac aat ttt gaa aac gcg gat       1056
Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp           
            340                 345                 350                   

cgc gat ccc act tac gag gcc cgc aag gga gga gag cct gtt tgt tgg       1104
Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp           
        355                 360                 365                       

ttc aag tcg caa gtc gaa acc tcg tca act tac gga ggt ttc atc tcg       1152
Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser           
    370                 375                 380                           

ggt tgc ttg acg ggc gga ctc aac ttc caa gtg gaa cac cac ttg ttc       1200
Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe           
385                 390                 395                 400           

cct cgt atg agt tcg gcc tgg tac ccc tac att gcc cct act gtt cga       1248
Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg           
                405                 410                 415               

gag gtt tgc aaa aag cac gga gtc aag tac gca tac tat ccc tgg gtc       1296
Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val           
            420                 425                 430                   

tgg caa aac ttg att tca act gtc aag tat ctg cat caa agc gga act       1344
Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr           
        435                 440                 445                       

gga tcc aac tgg aag aat ggc gcc aac ccc tac tcg gga aaa ttg taa       1392
Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu               
    450                 455                 460                           


<210>  74
<211>  463
<212>  PRT
<213>  Peridinium sp. CCMP626

<400>  74

Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile 
1               5                   10                  15      


Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu 
            20                  25                  30          


Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp 
        35                  40                  45              


Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly 
    50                  55                  60                  


Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser 
65                  70                  75                  80  


Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr 
                85                  90                  95      


Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln 
            100                 105                 110         


Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly 
        115                 120                 125             


Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln 
    130                 135                 140                 


Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr 
145                 150                 155                 160 


Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn 
                165                 170                 175     


His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu 
            180                 185                 190         


Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His 
        195                 200                 205             


Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala 
    210                 215                 220                 


Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His 
225                 230                 235                 240 


Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe 
                245                 250                 255     


Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu 
            260                 265                 270         


Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn 
        275                 280                 285             


Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu 
    290                 295                 300                 


Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser 
305                 310                 315                 320 


Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu 
                325                 330                 335     


Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp 
            340                 345                 350         


Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp 
        355                 360                 365             


Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser 
    370                 375                 380                 


Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe 
385                 390                 395                 400 


Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg 
                405                 410                 415     


Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val 
            420                 425                 430         


Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr 
        435                 440                 445             


Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu 
    450                 455                 460             


<210>  75
<211>  1392
<212>  DNA
<213>  Peridinium sp. CCMP626


<220>
<221>  CDS
<222>  (1)..(1392)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136646
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1392)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0271632-A1
<311>  2007-05-15
<312>  2007-11-22
<313>  (1)..(1392)

<400>  75
atg gct ccc gac gcc gac aag ctg cga cag cga aag gct cag tcc atc         48
Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile           
1               5                   10                  15                

cag gac act gcc gat tct cag gct acc gag ctc aag att ggc acc ctg         96
Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu           
            20                  25                  30                    

aag ggt ctc caa ggc acc gag atc gtc att gat ggc gac atc tac gac        144
Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp           
        35                  40                  45                        

atc aaa gac ttc gat cac cct gga ggc gaa tcc atc atg acc ttt ggt        192
Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly           
    50                  55                  60                            

ggc aac gac gtt act gcc acc tac aag atg att cat ccc tac cac tcg        240
Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser           
65                  70                  75                  80            

aag cat cac ctg gag aag atg aaa aag gtc ggt cga gtg ccc gac tac        288
Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr           
                85                  90                  95                

acc tcc gag tac aag ttc gat act ccc ttc gaa cga gag atc aaa cag        336
Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln           
            100                 105                 110                   

gag gtc ttc aag att gtg cga aga ggt cga gag ttt gga aca cct ggc        384
Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly           
        115                 120                 125                       

tac ttc ttt cga gcc ttc tgc tac atc ggt ctc ttc ttt tac ctg cag        432
Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln           
    130                 135                 140                           

tat ctc tgg gtt acc act cct acc act ttc gcc ctt gct atc ttc tac        480
Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr           
145                 150                 155                 160           

ggt gtg tct cag gcc ttc att ggc ctg aac gtc cag cac gac gcc aac        528
Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn           
                165                 170                 175               

cac gga gct gcc tcc aaa aag ccc tgg atc aac aat ttg ctc ggc ctg        576
His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu           
            180                 185                 190                   

ggt gcc gac ttt atc gga ggc tcc aag tgg ctc tgg atg aac cag cac        624
Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His           
        195                 200                 205                       

tgg acc cat cac act tac acc aac cat cac gag aag gat ccc gac gcc        672
Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala           
    210                 215                 220                           

ctg ggt gca gag cct atg ctg ctc ttc aac gac tat ccc ttg ggt cac        720
Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His           
225                 230                 235                 240           

ccc aag cga acc ctc att cat cac ttc caa gcc ttc tac tat ctg ttt        768
Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe           
                245                 250                 255               

gtc ctt gct ggc tac tgg gtg tct tcg gtg ttc aac cct cag atc ctg        816
Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu           
            260                 265                 270                   

gac ctc cag cac cga ggt gcc cag gct gtc ggc atg aag atg gag aac        864
Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn           
        275                 280                 285                       

gac tac att gcc aag tct cga aag tac gct atc ttc ctg cga ctc ctg        912
Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu           
    290                 295                 300                           

tac atc tac acc aac att gtg gct ccc atc cag aac caa ggc ttt tcg        960
Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser           
305                 310                 315                 320           

ctc acc gtc gtt gct cac att ctt act atg ggt gtc gcc tcc agc ctg       1008
Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu           
                325                 330                 335               

acc ctc gct act ctg ttc gcc ctc tcc cac aac ttc gag aac gca gat       1056
Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp           
            340                 345                 350                   

cgg gat ccc acc tac gag gct cga aag gga ggc gag cct gtc tgt tgg       1104
Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp           
        355                 360                 365                       

ttc aag tcg cag gtg gaa acc tcc tct act tac ggt ggc ttc att tcc       1152
Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser           
    370                 375                 380                           

ggt tgc ctt aca ggc gga ctc aac ttt cag gtc gag cat cac ctg ttt       1200
Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe           
385                 390                 395                 400           

cct cga atg tcc tct gcc tgg tac ccc tac atc gct cct acc gtt cga       1248
Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg           
                405                 410                 415               

gag gtc tgc aaa aag cac ggc gtc aag tac gcc tac tat ccc tgg gtg       1296
Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val           
            420                 425                 430                   

tgg cag aac ctc atc tcg acc gtc aag tac ctg cat cag tcc gga act       1344
Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr           
        435                 440                 445                       

ggc tcg aac tgg aag aac ggt gcc aat ccc tac tct ggc aag ctg taa       1392
Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu               
    450                 455                 460                           


<210>  76
<211>  463
<212>  PRT
<213>  Peridinium sp. CCMP626

<400>  76

Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile 
1               5                   10                  15      


Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu 
            20                  25                  30          


Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp 
        35                  40                  45              


Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly 
    50                  55                  60                  


Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser 
65                  70                  75                  80  


Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr 
                85                  90                  95      


Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln 
            100                 105                 110         


Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly 
        115                 120                 125             


Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln 
    130                 135                 140                 


Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr 
145                 150                 155                 160 


Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn 
                165                 170                 175     


His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu 
            180                 185                 190         


Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His 
        195                 200                 205             


Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala 
    210                 215                 220                 


Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His 
225                 230                 235                 240 


Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe 
                245                 250                 255     


Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu 
            260                 265                 270         


Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn 
        275                 280                 285             


Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu 
    290                 295                 300                 


Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser 
305                 310                 315                 320 


Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu 
                325                 330                 335     


Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp 
            340                 345                 350         


Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp 
        355                 360                 365             


Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser 
    370                 375                 380                 


Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe 
385                 390                 395                 400 


Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg 
                405                 410                 415     


Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val 
            420                 425                 430         


Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr 
        435                 440                 445             


Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu 
    450                 455                 460             


<210>  77
<211>  1362
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  delta-5 desaturase

<300>
<302>  DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  U.S. 2008-0274521-A1
<311>  2008-04-29
<312>  2008-11-06
<313>  (1)..(1362)

<300>
<302>  DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  WO 2008/137532
<311>  2008-05-01
<312>  2008-11-13
<313>  (1)..(1362)

<400>  77
atg gcc acc atc tct ttg act act gag caa ctt tta gaa cac cca gaa         48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu           
1               5                   10                  15                

ctg gtt gca att gat ggg gtg ttg tac gat ctc ttc gga ctg gcg aaa         96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys           
            20                  25                  30                    

gtg cat cca ggt ggc aac ctc att gaa gcc gcc ggt gcc tcc gac gga        144
Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly           
        35                  40                  45                        

acc gcc ctg ttc tac tcc atg cac cct gga gtg aag cca gag aat tcg        192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser           
    50                  55                  60                            

aag ctg ctg cag caa ttt gcc cga ggc aaa cac gaa cga agc tcg aag        240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys           
65                  70                  75                  80            

gac cca gtg tac acc ttt gac agt ccc ttc gcc cag gat gtc aag cag        288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln           
                85                  90                  95                

agc gtt cgg gag gtc atg aag ggg cgc aac tgg tac gcc acg ccc ggc        336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly           
            100                 105                 110                   

ttt tgg ctg cgg acc gcg ctg atc atc gcg tgc act gcc ata ggc gaa        384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu           
        115                 120                 125                       

tgg tat tgg atc act acc ggg gca gtg atg tgg ggc atc ttc acc ggg        432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly           
    130                 135                 140                           

tac ttc cac agc cag att ggg ttg gcg att caa cac gat gcc tct cac        480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His           
145                 150                 155                 160           

gga gcc atc agc aaa aag ccc tgg gtg aac gcc ttt ttc gcc tac ggc        528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly           
                165                 170                 175               

atc gac gcc att gga tcc tcc cgc tgg atc tgg ctg cag tcc cac att        576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile           
            180                 185                 190                   

atg cgc cac cac acc tac acc aac cag cat ggc ctg gac ctg gac gct        624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala           
        195                 200                 205                       

gcc tcg gcg gag ccg ttc att ttg ttc cac tcc tac ccg gca aca aat        672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn           
    210                 215                 220                           

gcg tca cga aag tgg tac cat cgg ttc cag gcg tgg tac atg tac atc        720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile           
225                 230                 235                 240           

gtt ttg ggg atg tat ggt gtg tcg atg gtg tac aat ccg atg tac ttg        768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu           
                245                 250                 255               

ttc acg atg cag cac aac gac aca atc cca gag gcc acc tct ctt aga        816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg           
            260                 265                 270                   

cca ggc agc ttt ttc aac cgg cag cgc gcc ttc gcc gtt tcc ctc cgc        864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg           
        275                 280                 285                       

cta ctg ttc atc ttc cgc aac gcc ttc ctc ccc tgg tac atc gcg ggc        912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly           
    290                 295                 300                           

gcc tct ccg ctg ctc acc atc ctg ctg gtg cca acg gtc aca ggc atc        960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile           
305                 310                 315                 320           

ttc ttg aca ttt gtt ttt gtg ctg tcc cat aac ttt gaa ggc gct gag       1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu           
                325                 330                 335               

cgg acc ccc gaa aag aac tgc aag gcc aaa agg gcc aag gag ggg aag       1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys           
            340                 345                 350                   

gag gtc cgc gat gta gag gag gac cgg gtg gac tgg tac cgg gcg cag       1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln           
        355                 360                 365                       

gcc gag acc gcg gcg acc tac ggg ggc agc gtc ggg atg atg ctg acc       1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr           
    370                 375                 380                           

ggc ggt ttg aac ctg cag atc gag cac cac ttg ttc ccc cgc atg tcc       1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser           
385                 390                 395                 400           

tct tgg cac tac ccc ttc atc caa gat acg gtg cgg gaa tgt tgc aag       1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys           
                405                 410                 415               

cgc cat ggc gtg cgc tac aca tac tac ccg acc atc ctg gag aat ata       1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile           
            420                 425                 430                   

atg tcc acg ctc cgc tac atg cag aag gtg ggc gtg gcc cac aca att       1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile           
        435                 440                 445                       

cag gat gcc cag gaa ttc                                               1362
Gln Asp Ala Gln Glu Phe                                                   
    450                                                                   


<210>  78
<211>  454
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  78

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  79
<211>  1362
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  U.S. 2008-0274521-A1
<311>  2008-04-29
<312>  2008-11-06
<313>  (1)..(1362)

<300>
<302>  DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  WO 2008/137532
<311>  2008-05-01
<312>  2008-11-13
<313>  (1)..(1362)

<400>  79
atg gcc acc atc tcc ctg act acc gag cag ctc ctg gaa cac ccc gag         48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu           
1               5                   10                  15                

ctc gtt gcc atc gac gga gtc ctg tac gat ctc ttc ggt ctg gcc aag         96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys           
            20                  25                  30                    

gtg cat cca gga ggc aac ctc atc gaa gct gcc ggt gca tcc gac gga        144
Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly           
        35                  40                  45                        

acc gct ctg ttc tac tcc atg cat cct gga gtc aag cca gag aac tcg        192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser           
    50                  55                  60                            

aag ctt ctg cag caa ttt gcc cga ggc aag cac gaa cga agc tcc aag        240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys           
65                  70                  75                  80            

gat ccc gtg tac acc ttc gac tct ccc ttt gct cag gac gtc aag cag        288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln           
                85                  90                  95                

tcc gtt cga gag gtc atg aag ggt cga aac tgg tac gcc act cct ggc        336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly           
            100                 105                 110                   

ttc tgg ctg aga acc gca ctc atc atc gct tgt act gcc att ggc gag        384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu           
        115                 120                 125                       

tgg tac tgg atc aca acc gga gca gtg atg tgg ggt atc ttt act gga        432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly           
    130                 135                 140                           

tac ttc cac tcg cag att ggc ttg gcc att caa cac gat gct tct cac        480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His           
145                 150                 155                 160           

gga gcc atc agc aaa aag ccc tgg gtc aac gcc ttt ttc gct tat ggc        528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly           
                165                 170                 175               

atc gac gcc att ggt tcc tct cgt tgg atc tgg ctg cag tcc cac att        576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile           
            180                 185                 190                   

atg cga cat cac act tac acc aac cag cat ggc ctc gac ctg gat gct        624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala           
        195                 200                 205                       

gcc tcg gca gag ccg ttc atc ttg ttc cac tcc tat cct gct acc aac        672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn           
    210                 215                 220                           

gcc tct cga aag tgg tac cac cga ttt cag gcg tgg tac atg tac atc        720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile           
225                 230                 235                 240           

gtt ctg gga atg tat ggt gtc tcg atg gtg tac aat ccc atg tac ctc        768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu           
                245                 250                 255               

ttc aca atg cag cac aac gac acc att ccc gag gcc act tct ctc aga        816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg           
            260                 265                 270                   

cca ggc agc ttt ttc aat cgg cag cga gct ttc gcc gtt tcc ctt cga        864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg           
        275                 280                 285                       

ctg ctc ttc atc ttc cga aac gcc ttt ctt ccc tgg tac att gct ggt        912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly           
    290                 295                 300                           

gcc tct cct ctg ctc acc att ctt ctg gtg ccc acg gtc aca ggc atc        960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile           
305                 310                 315                 320           

ttc ctc acc ttt gtg ttc gtt ctg tcc cat aac ttc gag gga gcc gaa       1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu           
                325                 330                 335               

cgg acc cca gag aag aac tgc aag gcc aaa cga gct aag gaa ggc aag       1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys           
            340                 345                 350                   

gag gtc aga gac gtg gaa gag gat cga gtc gac tgg tac cga gca cag       1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln           
        355                 360                 365                       

gcc gag act gct gcc acc tac ggt ggc agc gtg gga atg atg ctt aca       1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr           
    370                 375                 380                           

ggc ggt ctc aac ctg cag atc gag cat cac ttg ttt ccc cga atg tcc       1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser           
385                 390                 395                 400           

tct tgg cac tat ccc ttc att caa gac acc gtt cgg gag tgt tgc aag       1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys           
                405                 410                 415               

cga cat ggc gtc cgt tac aca tac tat cct acc att ctc gag aac atc       1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile           
            420                 425                 430                   

atg tcc act ctt cga tac atg cag aag gtg ggt gtt gct cac acc att       1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile           
        435                 440                 445                       

cag gat gcc cag gag ttc                                               1362
Gln Asp Ala Gln Glu Phe                                                   
    450                                                                   


<210>  80
<211>  454
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  80

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  81
<211>  1365
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1365)
<223>  synthetic mutant delta-5 desaturase (codon-optimized for Yarrowia
       lipolytica)

<400>  81
atg gcc acc atc tcc ctg act acc gag cag ctc ctg gaa cac ccc gag         48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu           
1               5                   10                  15                

ctc gtt gcc atc gac gga gtc ctg tac gat ctc ttc ggt ctg gcc aag         96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys           
            20                  25                  30                    

gtc cat gcc gga ggc aac ctc atc gaa gct gcc ggt gca tcc gac gga        144
Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly           
        35                  40                  45                        

acc gct ctg ttc tac tcc atg cat cct gga gtc aag cca gag aac tcg        192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser           
    50                  55                  60                            

aag ctt ctg cag caa ttt gcc cga ggc aag cac gaa cga agc tcc aag        240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys           
65                  70                  75                  80            

gat ccc gtg tac acc ttc gac tct ccc ttt gct cag gac gtc aag cag        288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln           
                85                  90                  95                

tcc gtt cga gag gtc atg aag ggt cga aac tgg tac gcc act cct ggc        336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly           
            100                 105                 110                   

ttc tgg ctg aga acc gca ctc atc atc gct tgt act gcc att ggc gag        384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu           
        115                 120                 125                       

tgg tac tgg atc aca acc gga gca gtg atg tgg ggt atc ttt act gga        432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly           
    130                 135                 140                           

tac ttc cac tcg cag att ggc ttg gcc att caa cac gat gct tct cac        480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His           
145                 150                 155                 160           

gga gcc atc agc aaa aag ccc tgg gtc aac gcc ttt ttc gct tat ggc        528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly           
                165                 170                 175               

atc gac gcc att ggt tcc tct cgt tgg atc tgg ctg cag tcc cac att        576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile           
            180                 185                 190                   

atg cga cat cac act tac acc aac cag cat ggc ctc gac ctg gat gct        624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala           
        195                 200                 205                       

gcc tcg gca gag ccg ttc atc ttg ttc cac tcc tat cct gct acc aac        672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn           
    210                 215                 220                           

gcc tct cga aag tgg tac cac cga ttt cag gcg tgg tac atg tac atc        720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile           
225                 230                 235                 240           

gtt ctg gga atg tat ggt gtc tcg atg gtg tac aat ccc atg tac ctc        768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu           
                245                 250                 255               

ttc aca atg cag cac aac gac acc att ccc gag gcc act tct ctc aga        816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg           
            260                 265                 270                   

cca ggc agc ttt ttc aat cgg cag cga gct ttc gcc gtt tcc ctt cga        864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg           
        275                 280                 285                       

ctg ctc ttc atc ttc cga aac gcc ttt ctt ccc tgg tac att gct ggt        912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly           
    290                 295                 300                           

gcc tct cct ctg ctc acc att ctt ctg gtg ccc acg gtc aca ggc atc        960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile           
305                 310                 315                 320           

ttc ctc acc ttt gtg ttc gtt ctg tcc cat aac ttc gag gga gcc gaa       1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu           
                325                 330                 335               

cgg acc cca gag aag aac tgc aag gcc aaa cga gct aag gaa ggc aag       1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys           
            340                 345                 350                   

gag gtc aga gac gtg gaa gag gat cga gtc gac tgg tac cga gca cag       1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln           
        355                 360                 365                       

gcc gag act gct gcc acc tac ggt ggc agc gtg gga atg atg ctt aca       1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr           
    370                 375                 380                           

ggc ggt ctc aac ctg cag atc gag cat cac ttg ttt ccc cga atg tcc       1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser           
385                 390                 395                 400           

tct tgg cac tat ccc ttc att caa gac acc gtt cgg gag tgt tgc aag       1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys           
                405                 410                 415               

cga cat ggc gtc cgt tac aca tac tat cct acc att ctc gag aac atc       1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile           
            420                 425                 430                   

atg tcc act ctt cga tac atg cag aag gtg ggt gtt gct cac acc att       1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile           
        435                 440                 445                       

cag gat gcc cag gag ttc taa                                           1365
Gln Asp Ala Gln Glu Phe                                                   
    450                                                                   


<210>  82
<211>  454
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  82

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  83
<211>  1086
<212>  DNA
<213>  Phytophthora ramorum


<220>
<221>  CDS
<222>  (1)..(1086)
<223>  delta-17 desaturase

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  WO 2007/123999
<311>  2007-04-19
<312>  2007-11-01
<313>  (1)..(1086)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  US 2007-0249026-A1
<311>  2007-04-18
<312>  2007-10-25
<313>  (1)..(1086)

<400>  83
atg gcg act aag cag ccg tac cag ttc ccg acc ctg acg gag atc aag         48
Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys           
1               5                   10                  15                

cgg tcg ctg ccc agc gag tgc ttt gag gcc tcg gtg ccg ctg tcg ctc         96
Arg Ser Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu           
            20                  25                  30                    

tac tac acg gtg cgc atc gtg gcc atc gcc gtg gcg ctg gcg ttc ggc        144
Tyr Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly           
        35                  40                  45                        

ctc aac tac gcg cgc gcg ctg ccc gtg gtc gag agc ttg tgg gcg ctg        192
Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu           
    50                  55                  60                            

gac gct gcg ctc tgc tgc ggt tac gtg ctg ctg cag ggc atc gtg ttc        240
Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe           
65                  70                  75                  80            

tgg ggc ttc ttc acg gtg ggc cat gac gcc ggc cac ggc gcc ttc tcg        288
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser           
                85                  90                  95                

cgt tac cac ctg ctc aac ttc gtg gtg ggc acc ttc atc cac tcg ctc        336
Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser Leu           
            100                 105                 110                   

atc ctc acg ccc ttc gag tcg tgg aag ctc acg cac cgc cac cac cac        384
Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His           
        115                 120                 125                       

aag aac acg ggc aac att gac cgc gac gag atc ttc tac ccg cag cgc        432
Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg           
    130                 135                 140                           

aag gcc gac gac cac ccg ctg tcg cgc aac ctc gtg ctg gcg ctc ggc        480
Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly           
145                 150                 155                 160           

gcc gcg tgg ttc gcc tac ctg gtc gag ggc ttc ccg ccc cgc aag gtc        528
Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val           
                165                 170                 175               

aac cac ttc aac cca ttc gag ccg ctg ttt gtg cgc cag gtg gcc gcc        576
Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala           
            180                 185                 190                   

gtc gtc atc tcg ctc tcc gcg cac ttc gcc gtg ttg gcg ctg tcc gtg        624
Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val           
        195                 200                 205                       

tat ctg agc ttc cag ttc ggt ctc aag acc atg gcg ctc tac tac tac        672
Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr           
    210                 215                 220                           

ggc ccc gtc ttc gtg ttc ggc agc atg ctt gtg atc acc acc ttc ctg        720
Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu           
225                 230                 235                 240           

cat cac aat gac gag gag acc cca tgg tac gga gac tcc gac tgg acc        768
His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp Ser Asp Trp Thr           
                245                 250                 255               

tac gtc aag ggc aac ctg tcg tcc gtg gac cgg tcc tac ggc gcg ttc        816
Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe           
            260                 265                 270                   

atc gac aac ctg agc cac aac atc ggc acg cac cag atc cac cac ctc        864
Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu           
        275                 280                 285                       

ttc ccc atc atc ccg cac tac aag ctc aac cgc gct acg gcg gca ttc        912
Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe           
    290                 295                 300                           

cac cag gcc ttc ccc gag ctc gtg cgc aag agc gac gag ccg atc ctc        960
His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu           
305                 310                 315                 320           

aag gcc ttc tgg cgc gtc ggc cga ctg tac gcc aac tac ggc gtc gtg       1008
Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val           
                325                 330                 335               

gac ccg gac gcc aag ctc ttc acg ctc aag gag gcc aag gcg gcg tcc       1056
Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser           
            340                 345                 350                   

gag gcg gcg acc aag acc aag gcc acc taa                               1086
Glu Ala Ala Thr Lys Thr Lys Ala Thr                                       
        355                 360                                           


<210>  84
<211>  361
<212>  PRT
<213>  Phytophthora ramorum

<400>  84

Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys 
1               5                   10                  15      


Arg Ser Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu 
            20                  25                  30          


Tyr Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly 
        35                  40                  45              


Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu 
    50                  55                  60                  


Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe 
65                  70                  75                  80  


Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 
                85                  90                  95      


Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser Leu 
            100                 105                 110         


Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His 
        115                 120                 125             


Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg 
    130                 135                 140                 


Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly 
145                 150                 155                 160 


Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val 
                165                 170                 175     


Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala 
            180                 185                 190         


Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val 
        195                 200                 205             


Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr 
    210                 215                 220                 


Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu 
225                 230                 235                 240 


His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp Ser Asp Trp Thr 
                245                 250                 255     


Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe 
            260                 265                 270         


Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu 
        275                 280                 285             


Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe 
    290                 295                 300                 


His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu 
305                 310                 315                 320 


Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 
                325                 330                 335     


Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser 
            340                 345                 350         


Glu Ala Ala Thr Lys Thr Lys Ala Thr 
        355                 360     


<210>  85
<211>  1086
<212>  DNA
<213>  Phytophthora ramorum


<220>
<221>  CDS
<222>  (1)..(1086)
<223>  synthetic delta-17 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  WO 2007/123999
<311>  2007-04-19
<312>  2007-11-01
<313>  (1)..(1086)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  US 2007-0249026-A1
<311>  2007-04-18
<312>  2007-10-25
<313>  (1)..(1086)

<400>  85
atg gct acc aag cag ccc tac cag ttc cct act ctg acc gag atc aag         48
Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys           
1               5                   10                  15                

cga tct ctt ccc tcc gag tgc ttt gaa gcc tcg gtc cct ctg tcc ttg         96
Arg Ser Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu           
            20                  25                  30                    

tac tac acc gtg cga atc gtc gct att gcc gtt gct ctg gcc ttc gga        144
Tyr Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly           
        35                  40                  45                        

ctc aac tac gct cga gcc ctt ccc gtg gtc gag tct ctg tgg gca ctc        192
Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu           
    50                  55                  60                            

gac gct gcc ctt tgt tgc ggt tac gtt ctg ctc caa ggc att gtc ttc        240
Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe           
65                  70                  75                  80            

tgg gga ttc ttt acc gtg ggt cac gat gct gga cat ggt gcc ttc tct        288
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser           
                85                  90                  95                

cga tac cac ctg ctc aac ttt gtc gtt ggc acc ttt atc cac tcc ctc        336
Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser Leu           
            100                 105                 110                   

att ctt act ccc ttc gag tcg tgg aag ctc aca cat cga cac cat cac        384
Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His           
        115                 120                 125                       

aag aac acc gga aac atc gac cga gac gaa atc ttc tac cct cag cga        432
Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg           
    130                 135                 140                           

aag gcc gac gat cat cct ctg tct cga aac ctc gtc ctg gct ctc ggt        480
Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly           
145                 150                 155                 160           

gcc gct tgg ttt gcc tac ctt gtc gag ggc ttt cct ccc cga aag gtc        528
Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val           
                165                 170                 175               

aac cac ttc aac ccc ttc gaa cct ctg ttt gtg cga cag gtg gct gcc        576
Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala           
            180                 185                 190                   

gtt gtc att tcc ctc tct gct cac ttc gcc gtc ctg gca ctg tcc gtg        624
Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val           
        195                 200                 205                       

tat ctg agc ttt cag ttc ggt ctc aag aca atg gct ctg tac tac tat        672
Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr           
    210                 215                 220                           

gga ccc gtc ttc gtg ttc ggc tcc atg ctc gtc att act acc ttt ctg        720
Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu           
225                 230                 235                 240           

cat cac aat gac gag gaa act cct tgg tac gga gat tcc gac tgg acc        768
His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp Ser Asp Trp Thr           
                245                 250                 255               

tac gtc aag ggc aac ttg tct tcc gtg gac cga tct tac ggt gcc ttc        816
Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe           
            260                 265                 270                   

atc gac aac ctc tcg cac aac att ggc aca cac cag atc cac cat ctg        864
Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu           
        275                 280                 285                       

ttt ccc atc att cct cac tac aag ctc aac cga gcc acc gct gcc ttc        912
Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe           
    290                 295                 300                           

cac cag gcc ttt ccc gaa ctt gtc cga aag agc gac gag ccc att ctc        960
His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu           
305                 310                 315                 320           

aag gct ttc tgg aga gtt ggt cga ctt tac gcc aac tac gga gtc gtg       1008
Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val           
                325                 330                 335               

gat ccc gac gca aag ctg ttt act ctc aag gag gcc aaa gct gcc tcc       1056
Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser           
            340                 345                 350                   

gag gct gcc acc aag acc aag gct act taa                               1086
Glu Ala Ala Thr Lys Thr Lys Ala Thr                                       
        355                 360                                           


<210>  86
<211>  361
<212>  PRT
<213>  Phytophthora ramorum

<400>  86

Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys 
1               5                   10                  15      


Arg Ser Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu 
            20                  25                  30          


Tyr Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly 
        35                  40                  45              


Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu 
    50                  55                  60                  


Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe 
65                  70                  75                  80  


Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 
                85                  90                  95      


Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser Leu 
            100                 105                 110         


Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His 
        115                 120                 125             


Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg 
    130                 135                 140                 


Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly 
145                 150                 155                 160 


Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val 
                165                 170                 175     


Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala 
            180                 185                 190         


Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val 
        195                 200                 205             


Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr 
    210                 215                 220                 


Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu 
225                 230                 235                 240 


His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp Ser Asp Trp Thr 
                245                 250                 255     


Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe 
            260                 265                 270         


Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu 
        275                 280                 285             


Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe 
    290                 295                 300                 


His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu 
305                 310                 315                 320 


Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 
                325                 330                 335     


Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser 
            340                 345                 350         


Glu Ala Ala Thr Lys Thr Lys Ala Thr 
        355                 360     


<210>  87
<211>  1080
<212>  DNA
<213>  Pythium aphanidermatum


<220>
<221>  CDS
<222>  (1)..(1080)
<223>  delta-17 desaturase

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  WO 2008/054565
<311>  2007-07-19
<312>  2008-05-08
<313>  (1)..(1080)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  US 2008-0125326-A1
<311>  2007-07-19
<312>  2008-05-29
<313>  (1)..(1080)

<400>  87
atg gct tct tcc act gtt gct gcg ccg tac gag ttc ccg acg ctg acg         48
Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr           
1               5                   10                  15                

gag atc aag cgc tcg ctg cca gcg cac tgc ttt gag gcc tcg gtc ccg         96
Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro           
            20                  25                  30                    

tgg tcg ctc tac tac acc gtg cgc gcg ctg ggc atc gcc ggc tcg ctc        144
Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu           
        35                  40                  45                        

gcg ctc ggc ctc tac tac gcg cgc gcg ctc gcg atc gtg cag gag ttt        192
Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe           
    50                  55                  60                            

gcc ctg ctg gat gcg gtg ctc tgc acg ggg tac att ctg ctg cag ggc        240
Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly           
65                  70                  75                  80            

atc gta ttc tgg ggg ttc ttc acc atc ggc cat gac tgc ggc cac ggc        288
Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly           
                85                  90                  95                

gcg ttc tcg cgt tcg cac ctg ctc aac ttc agc gtc ggc acg ctc att        336
Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile           
            100                 105                 110                   

cac tcg atc atc ctc acg ccg tac gag tca tgg aag atc tcg cac cgc        384
His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg           
        115                 120                 125                       

cac cac cac aag aac acg ggc aac atc gac aag gac gag att ttc tac        432
His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr           
    130                 135                 140                           

ccg cag cgc gag gcc gac tcg cac cca ctg tcc cga cac atg gtg atc        480
Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile           
145                 150                 155                 160           

tcg ctc ggc tcg gcc tgg ttc gcg tac ctc gtt gcg ggc ttc cct cct        528
Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro           
                165                 170                 175               

cgc aag gtg aac cac ttc aac cct tgg gaa ccg ttg tac ctg cgc cgc        576
Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg           
            180                 185                 190                   

atg tct gcc gtc atc atc tca ctc ggc tcg ctc gtg gcg ttc gcg ggc        624
Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly           
        195                 200                 205                       

ttg tat gcg tat ctc acc tac gtc tat ggc ctt aag acc atg gcg ctg        672
Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu           
    210                 215                 220                           

tac tac ttc gcc cct ctc ttt ggg ttc gcc acg atg ctc gtg gtc act        720
Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr           
225                 230                 235                 240           

acc ttt ttg cac cac aat gac gag gaa acg cca tgg tac gcc gac tcg        768
Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser           
                245                 250                 255               

gag tgg acg tac gtc aag ggc aac ctc tcg tcc gtg gac cgc tcg tac        816
Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr           
            260                 265                 270                   

ggc gcg ctc atc gac aac ctg agc cac aac atc ggc acg cac cag atc        864
Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile           
        275                 280                 285                       

cac cac ctg ttt ccg atc atc ccg cac tac aag ctg aac gag gcg acg        912
His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr           
    290                 295                 300                           

gca gcg ttc gcg cag gcg ttc ccg gag ctc gtg cgc aag agc gcg tcg        960
Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser           
305                 310                 315                 320           

ccg atc atc ccg acg ttc atc cgc atc ggg ctc atg tac gcc aag tac       1008
Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr           
                325                 330                 335               

ggc gtc gtg gac aag gac gcc aag atg ttt acg ctc aag gag gcc aag       1056
Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys           
            340                 345                 350                   

gcc gcc aag acc aag gcc aac tag                                       1080
Ala Ala Lys Thr Lys Ala Asn                                               
        355                                                               


<210>  88
<211>  359
<212>  PRT
<213>  Pythium aphanidermatum

<400>  88

Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr 
1               5                   10                  15      


Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro 
            20                  25                  30          


Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu 
        35                  40                  45              


Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe 
    50                  55                  60                  


Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly 
65                  70                  75                  80  


Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly 
                85                  90                  95      


Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile 
            100                 105                 110         


His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg 
        115                 120                 125             


His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr 
    130                 135                 140                 


Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile 
145                 150                 155                 160 


Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro 
                165                 170                 175     


Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg 
            180                 185                 190         


Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly 
        195                 200                 205             


Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu 
    210                 215                 220                 


Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr 
225                 230                 235                 240 


Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser 
                245                 250                 255     


Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr 
            260                 265                 270         


Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile 
        275                 280                 285             


His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr 
    290                 295                 300                 


Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser 
305                 310                 315                 320 


Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr 
                325                 330                 335     


Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys 
            340                 345                 350         


Ala Ala Lys Thr Lys Ala Asn 
        355                 


<210>  89
<211>  1080
<212>  DNA
<213>  Pythium aphanidermatum


<220>
<221>  CDS
<222>  (1)..(1080)
<223>  synthetic delta-17 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  WO 2008/054565
<311>  2007-07-19
<312>  2008-05-08
<313>  (1)..(1080)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  US 2008-0125326-A1
<311>  2007-07-19
<312>  2008-05-29
<313>  (1)..(1080)

<400>  89
atg gct tcc tct acc gtt gcc gct ccc tac gag ttc cct act ctc acc         48
Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr           
1               5                   10                  15                

gag atc aag cga tcc ctg cct gcc cac tgc ttc gaa gcc tct gtt ccc         96
Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro           
            20                  25                  30                    

tgg tcc ctc tac tat acc gtg cga gct ctg ggc att gcc ggt tcc ctt        144
Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu           
        35                  40                  45                        

gct ctc gga ctg tac tat gct cga gcc ctt gct atc gtg cag gag ttt        192
Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe           
    50                  55                  60                            

gca ctg ctc gat gcc gtc ctt tgc act ggc tac att ctg ctc cag ggt        240
Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly           
65                  70                  75                  80            

atc gtg ttc tgg gga ttc ttt acc atc ggt cac gac tgt gga cat ggt        288
Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly           
                85                  90                  95                

gcc ttc tcg cga tcc cac ctg ctc aac ttc tct gtt ggc aca ctc att        336
Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile           
            100                 105                 110                   

cac tcc atc att ctg act ccc tac gag tcg tgg aag atc agc cat cga        384
His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg           
        115                 120                 125                       

cac cat cac aag aac acc ggc aac atc gac aag gat gag atc ttc tac        432
His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr           
    130                 135                 140                           

cct cag cga gaa gcc gac tct cat ccc ctg tcc cga cac atg gtc atc        480
Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile           
145                 150                 155                 160           

tcc ctt ggt tcg gct tgg ttt gcc tac ctc gtt gct gga ttt cct ccc        528
Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro           
                165                 170                 175               

cga aag gtc aac cac ttc aat ccc tgg gag cct ctc tac ctg cga aga        576
Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg           
            180                 185                 190                   

atg tct gcc gtc atc att tcc ctc ggc tct ctc gtg gcc ttt gct ggt        624
Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly           
        195                 200                 205                       

ctg tac gcc tac ctt acc tac gtc tac ggc ctc aag acc atg gct ctg        672
Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu           
    210                 215                 220                           

tat tac ttc gca cct ctc ttt gga ttc gcc acc atg ctg gtt gtc act        720
Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr           
225                 230                 235                 240           

acc ttc ctc cat cac aac gac gag gaa act ccc tgg tac gcc gat tcg        768
Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser           
                245                 250                 255               

gag tgg acc tat gtc aag ggc aac ttg tcc tct gtg gac cga agc tac        816
Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr           
            260                 265                 270                   

gga gcc ctc atc gac aac ctg tcc cac aac att ggt aca cat cag atc        864
Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile           
        275                 280                 285                       

cac cat ctg ttt ccc atc att cct cac tac aag ctc aac gag gcc act        912
His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr           
    290                 295                 300                           

gct gcc ttc gct cag gcc ttt ccc gaa ctg gtg cga aag tcg gct tct        960
Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser           
305                 310                 315                 320           

ccc atc att ccc acc ttc atc cga att ggt ctt atg tac gcc aag tac       1008
Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr           
                325                 330                 335               

ggc gtg gtc gac aag gat gcc aag atg ttt acc ctc aag gag gcc aag       1056
Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys           
            340                 345                 350                   

gct gcc aag acc aaa gcc aac taa                                       1080
Ala Ala Lys Thr Lys Ala Asn                                               
        355                                                               


<210>  90
<211>  359
<212>  PRT
<213>  Pythium aphanidermatum

<400>  90

Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr 
1               5                   10                  15      


Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro 
            20                  25                  30          


Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu 
        35                  40                  45              


Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe 
    50                  55                  60                  


Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly 
65                  70                  75                  80  


Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly 
                85                  90                  95      


Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile 
            100                 105                 110         


His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg 
        115                 120                 125             


His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr 
    130                 135                 140                 


Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile 
145                 150                 155                 160 


Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro 
                165                 170                 175     


Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg 
            180                 185                 190         


Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly 
        195                 200                 205             


Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu 
    210                 215                 220                 


Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr 
225                 230                 235                 240 


Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser 
                245                 250                 255     


Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr 
            260                 265                 270         


Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile 
        275                 280                 285             


His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr 
    290                 295                 300                 


Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser 
305                 310                 315                 320 


Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr 
                325                 330                 335     


Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys 
            340                 345                 350         


Ala Ala Lys Thr Lys Ala Asn 
        355                 


<210>  91
<211>  1434
<212>  DNA
<213>  Fusarium monoliforme


<220>
<221>  CDS
<222>  (1)..(1434)
<223>  delta-12 desaturase

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  WO 2005/047485
<311>  2004-11-12
<312>  2005-05-26
<313>  (1)..(1434)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  U.S. 7,504,259
<311>  2004-11-10
<312>  2009-03-17
<313>  (1)..(1434)

<400>  91
atg gcg tcc act tcg gct ctg ccc aag cag aac cct gcg ctt aga cgc         48
Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg           
1               5                   10                  15                

acc gtc acc tca act act gtg acg gat tct gag tct gcc gcc gtc tct         96
Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser           
            20                  25                  30                    

cct tca gac tct ccc cgc cac tcg gcc tct tcc aca tcg ctc tcg tcc        144
Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser           
        35                  40                  45                        

atg tcc gag gtt gat atc gcc aag ccc aag tcc gag tat ggt gtc atg        192
Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met           
    50                  55                  60                            

ctc gac acc tac ggc aac cag ttc gag gtt ccc gac ttt acc atc aag        240
Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys           
65                  70                  75                  80            

gac atc tac aat gcc atc cct aag cac tgc ttc aag cgc tcc gct ctc        288
Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu           
                85                  90                  95                

aag gga tac ggt tat atc ctc cgc gac att gtc ctc ctg act acc act        336
Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr           
            100                 105                 110                   

ttc agc atc tgg tac aac ttt gtg acc ccc gaa tat atc ccc tcc acc        384
Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr           
        115                 120                 125                       

ccc gcc cgc gct ggt ctg tgg gcc gtg tac acc gtt ctt cag ggt ctt        432
Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu           
    130                 135                 140                           

ttc ggt act ggt ctc tgg gtt att gcc cat gag tgc ggt cac ggt gct        480
Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala           
145                 150                 155                 160           

ttc tcc gat tct cgc atc atc aac gac att act ggc tgg gtt ctt cac        528
Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His           
                165                 170                 175               

tct tcc ctc ctt gtc ccc tac ttc agc tgg caa atc tcc cac cga aag        576
Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys           
            180                 185                 190                   

cac cac aag gcc act ggc aac atg gag cgt gac atg gtc ttc gtt ccc        624
His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro           
        195                 200                 205                       

cga acc cgc gag cag cag gct act cgt ctc gga aag atg acc cac gag        672
Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu           
    210                 215                 220                           

ctc gct cat ctt act gag gag acc ccc gct ttc act ctt ctc atg ctc        720
Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu           
225                 230                 235                 240           

gtc ctt cag cag ctc gtt ggc tgg ccc aac tac ctc atc acc aat gtt        768
Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val           
                245                 250                 255               

acc ggc cac aac tac cac gag cgc cag cgt gag ggt cgc ggc aag ggc        816
Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly           
            260                 265                 270                   

aag cat aac ggc ctc ggc ggt ggt gtt aac cac ttc gat ccc cgc agc        864
Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser           
        275                 280                 285                       

cct ctg tac gag aac agt gac gct aag ctc atc gtc ctc agc gat att        912
Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile           
    290                 295                 300                           

ggt atc ggt ctg atg gcc act gct ctg tac ttc ctc gtt cag aag ttc        960
Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe           
305                 310                 315                 320           

ggt ttc tac aac atg gcc atc tgg tac ttt gtt ccc tac ctc tgg gtt       1008
Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val           
                325                 330                 335               

aac cac tgg ctc gtt gcc atc acc ttc ctc cag cac acc gac cct acc       1056
Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr           
            340                 345                 350                   

ctt ccc cac tac acc aac gac gag tgg aac ttc gtc cgt ggt gcc gct       1104
Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala           
        355                 360                 365                       

gct acc att gac cgt gag atg ggc ttc atc ggc cgc cac ctt ctc cac       1152
Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His           
    370                 375                 380                           

ggc atc atc gag act cat gtc ctc cac cac tac gtc agc agc atc ccc       1200
Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro           
385                 390                 395                 400           

ttc tac aac gcg gac gag gcc acc gag gcc att aag ccc atc atg ggc       1248
Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly           
                405                 410                 415               

aag cac tac cgg gct gat gtc cag gat ggt cct cgt ggc ttc atc cgc       1296
Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg           
            420                 425                 430                   

gcc atg tac cgc agt gcg cgt atg tgc cag tgg gtt gag ccc agc gct       1344
Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala           
        435                 440                 445                       

ggt gcc gag ggt gct ggt aag ggt gtt ctg ttc ttc cgc aac cgc aac       1392
Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn           
    450                 455                 460                           

aac gtg ggc acc ccc ccc gct gtt atc aag ccc gtt gct taa               1434
Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala                       
465                 470                 475                               


<210>  92
<211>  477
<212>  PRT
<213>  Fusarium monoliforme

<400>  92

Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg 
1               5                   10                  15      


Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser 
            20                  25                  30          


Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser 
        35                  40                  45              


Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met 
    50                  55                  60                  


Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys 
65                  70                  75                  80  


Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu 
                85                  90                  95      


Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr 
            100                 105                 110         


Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr 
        115                 120                 125             


Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu 
    130                 135                 140                 


Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala 
145                 150                 155                 160 


Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His 
                165                 170                 175     


Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys 
            180                 185                 190         


His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro 
        195                 200                 205             


Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu 
    210                 215                 220                 


Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu 
225                 230                 235                 240 


Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val 
                245                 250                 255     


Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly 
            260                 265                 270         


Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser 
        275                 280                 285             


Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile 
    290                 295                 300                 


Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe 
305                 310                 315                 320 


Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val 
                325                 330                 335     


Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr 
            340                 345                 350         


Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala 
        355                 360                 365             


Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His 
    370                 375                 380                 


Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro 
385                 390                 395                 400 


Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly 
                405                 410                 415     


Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg 
            420                 425                 430         


Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala 
        435                 440                 445             


Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn 
    450                 455                 460                 


Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala 
465                 470                 475         


<210>  93
<211>  1434
<212>  DNA
<213>  Fusarium moniliforme


<220>
<221>  CDS
<222>  (1)..(1434)
<223>  synthetic delta-12 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  WO 2005/047485
<311>  2004-11-12
<312>  2005-05-26
<313>  (1)..(1434)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  U.S. 7,504,259
<311>  2004-11-10
<312>  2009-03-17
<313>  (1)..(1434)

<400>  93
atg gcc tcc acc tcg gct ctg ccc aag cag aac cct gcc ctc cga cga         48
Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg           
1               5                   10                  15                

acc gtc act tcc acc act gtg acc gac tcg gag tct gct gcc gtc tct         96
Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser           
            20                  25                  30                    

ccc tcc gat tct ccc aga cac tcg gcc tcc tct aca tcg ctg tct tcc        144
Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser           
        35                  40                  45                        

atg tcc gag gtg gac att gcc aag ccc aag tcc gag tac ggt gtc atg        192
Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met           
    50                  55                  60                            

ctg gat acc tac ggc aac cag ttc gaa gtt ccc gac ttc acc atc aag        240
Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys           
65                  70                  75                  80            

gac atc tac aac gct att ccc aag cac tgc ttc aag cga tct gct ctc        288
Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu           
                85                  90                  95                

aag gga tac ggc tac att ctt cga gac att gtc ctc ctg act acc act        336
Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr           
            100                 105                 110                   

ttc agc atc tgg tac aac ttt gtg aca ccc gag tac att ccc tcc act        384
Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr           
        115                 120                 125                       

cct gct cga gcc ggt ctg tgg gct gtg tac acc gtt ctt cag gga ctc        432
Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu           
    130                 135                 140                           

ttc ggt act gga ctg tgg gtc att gcc cac gag tgt gga cat ggt gct        480
Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala           
145                 150                 155                 160           

ttc tcc gat tcc cga atc atc aac gac att act ggc tgg gtg ctt cac        528
Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His           
                165                 170                 175               

tct tcc ctg ctt gtt ccc tac ttc agc tgg caa atc tcc cac cgg aag        576
Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys           
            180                 185                 190                   

cat cac aag gcc act gga aac atg gag cga gac atg gtc ttc gtt cct        624
His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro           
        195                 200                 205                       

cga acc cga gag cag caa gct act cga ctc ggc aag atg acc cac gaa        672
Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu           
    210                 215                 220                           

ctc gcc cat ctt acc gag gaa act cct gct ttc acc ctg ctc atg ctt        720
Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu           
225                 230                 235                 240           

gtg ctt cag caa ctg gtc ggt tgg ccc aac tat ctc att acc aac gtt        768
Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val           
                245                 250                 255               

act gga cac aac tac cat gag cgg cag cga gag ggt cga ggc aag gga        816
Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly           
            260                 265                 270                   

aag cac aac ggt ctt ggc ggt gga gtt aac cat ttc gat ccc cga tct        864
Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser           
        275                 280                 285                       

cct ctg tac gag aac agc gac gcc aag ctc atc gtg ctc tcc gac att        912
Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile           
    290                 295                 300                           

ggc att ggt ctt atg gcc acc gct ctg tac ttt ctc gtt cag aag ttc        960
Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe           
305                 310                 315                 320           

gga ttc tac aac atg gcc atc tgg tac ttc gtt ccc tac ttg tgg gtt       1008
Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val           
                325                 330                 335               

aac cac tgg ctc gtc gcc att acc ttt ctg cag cac aca gat cct act       1056
Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr           
            340                 345                 350                   

ctt ccc cac tac acc aac gac gag tgg aac ttt gtg cga ggt gcc gct       1104
Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala           
        355                 360                 365                       

gca acc atc gac cga gag atg ggc ttc att gga cgt cat ctg ctc cac       1152
Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His           
    370                 375                 380                           

ggc att atc gag act cac gtc ctg cat cac tac gtc tct tcc att ccc       1200
Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro           
385                 390                 395                 400           

ttc tac aat gcg gac gaa gct acc gag gcc atc aaa cct atc atg ggc       1248
Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly           
                405                 410                 415               

aag cac tat cga gct gat gtc cag gac ggt cct cga gga ttc att cga       1296
Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg           
            420                 425                 430                   

gcc atg tac cga tct gca cga atg tgc cag tgg gtt gaa ccc tcc gct       1344
Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala           
        435                 440                 445                       

ggt gcc gag gga gct ggc aag ggt gtc ctg ttc ttt cga aac cga aac       1392
Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn           
    450                 455                 460                           

aat gtg ggc act cct ccc gct gtc atc aag ccc gtt gcc taa               1434
Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala                       
465                 470                 475                               


<210>  94
<211>  477
<212>  PRT
<213>  Fusarium moniliforme

<400>  94

Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg 
1               5                   10                  15      


Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser 
            20                  25                  30          


Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser 
        35                  40                  45              


Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met 
    50                  55                  60                  


Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys 
65                  70                  75                  80  


Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu 
                85                  90                  95      


Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr 
            100                 105                 110         


Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr 
        115                 120                 125             


Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu 
    130                 135                 140                 


Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala 
145                 150                 155                 160 


Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His 
                165                 170                 175     


Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys 
            180                 185                 190         


His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro 
        195                 200                 205             


Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu 
    210                 215                 220                 


Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu 
225                 230                 235                 240 


Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val 
                245                 250                 255     


Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly 
            260                 265                 270         


Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser 
        275                 280                 285             


Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile 
    290                 295                 300                 


Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe 
305                 310                 315                 320 


Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val 
                325                 330                 335     


Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr 
            340                 345                 350         


Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala 
        355                 360                 365             


Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His 
    370                 375                 380                 


Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro 
385                 390                 395                 400 


Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly 
                405                 410                 415     


Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg 
            420                 425                 430         


Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala 
        435                 440                 445             


Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn 
    450                 455                 460                 


Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala 
465                 470                 475         


<210>  95
<211>  828
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(828)
<223>  C16/18 elongase

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  WO 2007/046817
<311>  2005-11-04
<312>  2007-04-26
<313>  (1)..(828)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  US 2007-0087420-A1
<311>  2005-10-19
<312>  2007-04-19
<313>  (1)..(828)

<400>  95
atg gag tct gga cct atg cct gcc ggg atc ccc ttc cct gaa tac tat         48
Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr           
1               5                   10                  15                

gac ttt ttc atg gac tgg aag aca ccc ctg gca att gct gcc acc tac         96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr           
            20                  25                  30                    

acc gcc gct gtt ggg ctc ttc aac ccc aag gtt ggc aaa gtc tcg cgc        144
Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg           
        35                  40                  45                        

gtg gta gcc aag tcg gct aac gcc aag ccg gca gag cgc acg cag tcc        192
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser           
    50                  55                  60                            

ggc gcc gcc atg acc gcc ttt gtc ttt gtc cac aac ctt atc ctc tgc        240
Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys           
65                  70                  75                  80            

gtg tac tct gga atc acc ttc tac tac atg ttc cca gcc atg gtc aag        288
Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys           
                85                  90                  95                

aac ttt aga aca cat acc ctc cat gag gcc tac tgc gat acg gat cag        336
Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln           
            100                 105                 110                   

agc ctg tgg aac aac gcc ctt ggc tac tgg ggc tac ctc ttc tac ctt        384
Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu           
        115                 120                 125                       

tca aag ttt tac gag gtc att gac acc atc atc atc atc ttg aag ggg        432
Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly           
    130                 135                 140                           

cgc cgc tcg tcc ctg ctc cag acc tac cac cac gcc ggc gct atg atc        480
Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile           
145                 150                 155                 160           

acc atg tgg tcc ggc atc aac tac cag gca acg ccc att tgg att ttt        528
Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe           
                165                 170                 175               

gtc gtc ttc aac tcg ttc atc cac acc atc atg tac tgt tac tat gcc        576
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala           
            180                 185                 190                   

ttc acc tca atc ggc ttc cac ccc cca ggc aag aag tac ctc acc tcc        624
Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser           
        195                 200                 205                       

atg cag atc acc cag ttt ttg gtc ggc atc act atc gcc gtc tct tat        672
Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr           
    210                 215                 220                           

ctc ttc gtc cct gga tgt atc cgc aca ccc ggt gct cag atg gct gtc        720
Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val           
225                 230                 235                 240           

tgg atc aac gtc gga tac ctc ttt ccc ctc act tat ctc ttt gtg gat        768
Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp           
                245                 250                 255               

ttt gcc aag cgt act tac tcc aag cgt agt gcc atc gcc gct cag aag        816
Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys           
            260                 265                 270                   

aag gcc cag taa                                                        828
Lys Ala Gln                                                               
        275                                                               


<210>  96
<211>  275
<212>  PRT
<213>  Mortierella alpina

<400>  96

Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 
1               5                   10                  15      


Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 
            20                  25                  30          


Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 
        35                  40                  45              


Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 
    50                  55                  60                  


Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys 
65                  70                  75                  80  


Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys 
                85                  90                  95      


Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln 
            100                 105                 110         


Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 
        115                 120                 125             


Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 
    130                 135                 140                 


Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 
145                 150                 155                 160 


Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 
                165                 170                 175     


Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 
            180                 185                 190         


Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser 
        195                 200                 205             


Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr 
    210                 215                 220                 


Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val 
225                 230                 235                 240 


Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 
                245                 250                 255     


Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 
            260                 265                 270         


Lys Ala Gln 
        275 


<210>  97
<211>  828
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(828)
<223>  synthetic C16/18 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  U.S. 7,470,532
<311>  2005-10-19
<312>  2008-12-30
<313>  (1)..(828)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  WO 2007/046817
<311>  2005-11-04
<312>  2007-04-26
<313>  (1)..(828)

<400>  97
atg gag tct gga ccc atg cct gct ggc att ccc ttc cct gag tac tat         48
Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr           
1               5                   10                  15                

gac ttc ttt atg gac tgg aag act ccc ctg gcc atc gct gcc acc tac         96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr           
            20                  25                  30                    

act gct gcc gtc ggt ctc ttc aac ccc aag gtt ggc aag gtc tcc cga        144
Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg           
        35                  40                  45                        

gtg gtt gcc aag tcg gct aac gca aag cct gcc gag cga acc cag tcc        192
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser           
    50                  55                  60                            

gga gct gcc atg act gcc ttc gtc ttt gtg cac aac ctc att ctg tgt        240
Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys           
65                  70                  75                  80            

gtc tac tct ggc atc acc ttc tac tac atg ttt cct gct atg gtc aag        288
Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys           
                85                  90                  95                

aac ttc cga acc cac aca ctg cac gaa gcc tac tgc gac acg gat cag        336
Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln           
            100                 105                 110                   

tcc ctc tgg aac aac gca ctt ggc tac tgg ggt tac ctc ttc tac ctg        384
Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu           
        115                 120                 125                       

tcc aag ttc tac gag gtc att gac acc atc atc atc atc ctg aag gga        432
Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly           
    130                 135                 140                           

cga cgg tcc tcg ctg ctt cag acc tac cac cat gct gga gcc atg att        480
Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile           
145                 150                 155                 160           

acc atg tgg tct ggc atc aac tac caa gcc act ccc att tgg atc ttt        528
Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe           
                165                 170                 175               

gtg gtc ttc aac tcc ttc att cac acc atc atg tac tgt tac tat gcc        576
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala           
            180                 185                 190                   

ttc acc tct atc gga ttc cat cct cct ggc aaa aag tac ctg act tcg        624
Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser           
        195                 200                 205                       

atg cag att act cag ttt ctg gtc ggt atc acc att gcc gtg tcc tac        672
Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr           
    210                 215                 220                           

ctc ttc gtt cct ggc tgc atc cga aca ccc ggt gct cag atg gct gtc        720
Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val           
225                 230                 235                 240           

tgg atc aac gtc ggc tac ctg ttt ccc ttg acc tat ctg ttc gtg gac        768
Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp           
                245                 250                 255               

ttt gcc aag cga acc tac tcc aag cga tct gcc att gcc gct cag aaa        816
Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys           
            260                 265                 270                   

aag gct cag taa                                                        828
Lys Ala Gln                                                               
        275                                                               


<210>  98
<211>  275
<212>  PRT
<213>  Mortierella alpina

<400>  98

Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 
1               5                   10                  15      


Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 
            20                  25                  30          


Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 
        35                  40                  45              


Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 
    50                  55                  60                  


Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys 
65                  70                  75                  80  


Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys 
                85                  90                  95      


Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln 
            100                 105                 110         


Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 
        115                 120                 125             


Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 
    130                 135                 140                 


Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 
145                 150                 155                 160 


Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 
                165                 170                 175     


Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 
            180                 185                 190         


Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser 
        195                 200                 205             


Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr 
    210                 215                 220                 


Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val 
225                 230                 235                 240 


Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 
                245                 250                 255     


Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 
            260                 265                 270         


Lys Ala Gln 
        275 


<210>  99
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  membrane bound O-acyltransferase motif


<220>
<221>  misc_feature
<222>  (4)..(6)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (9)..(11)
<223>  Xaa can be any naturally occurring amino acid

<300>
<301>  Hideo Shindou, Miki Etoa, Ryo Morimotoa and Takao Shimizua
<302>  Identification of membrane O-acyltransferase family motifs
<303>  Biochemical and Biophysical Research Communications
<304>  383
<305>  3
<306>  320-325
<307>  2009-04-08
<313>  (1)..(12)

<400>  99

Trp His Gly Xaa Xaa Xaa Gly Tyr Xaa Xaa Xaa Phe 
1               5                   10          


<210>  100
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  membrane bound O-acyltransferase motif


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<300>
<301>  Hideo Shindou, Miki Etoa, Ryo Morimotoa and Takao Shimizua
<302>  Identification of membrane O-acyltransferase family motifs
<303>  Biochemical and Biophysical Research Communications
<304>  383
<305>  3
<306>  320-325
<307>  2009-04-08
<313>  (1)..(6)

<400>  100

Tyr Xaa Xaa Xaa Xaa Phe 
1               5       


<210>  101
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  membrane bound O-acyltransferase motif


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Xaa can be any naturally occurring amino acid

<300>
<301>  Hideo Shindou, Miki Etoa, Ryo Morimotoa and Takao Shimizua
<302>  Identification of membrane O-acyltransferase family motifs
<303>  Biochemical and Biophysical Research Communications
<304>  383
<305>  3
<306>  320-325
<307>  2009-04-08
<313>  (1)..(9)

<400>  101

Tyr Xaa Xaa Xaa Tyr Phe Xaa Xaa His 
1               5                   


<210>  102
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = Val [V] or Ile [I]

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa = Leu [L] or Ile [I]

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Leu [L] or Ile [I] or Val [V]

<220>
<221>  misc_feature
<222>  (8)..(13)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  GENES ENCODING A NOVEL TYPE OF LYSOPHOPHATIDYLCHOLINE 
       ACYLTRANSFERASES AND THEIR USE TO INCREASE TRIACYLGLYCEROL 
       PRODUCTION AND/OR MODIFY FATTY ACID COMPOSITION
<310>  U.S. Patent Publication No. 2008-0145867-A1
<311>  2007-06-15
<312>  2008-06-19
<313>  (1)..(15)

<400>  102

Met Xaa Xaa Xaa Xaa Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asp Gly 
1               5                   10                  15  


<210>  103
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (9)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa = Glu [E] or Asp [D]

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa = Ala [A] or Gly [G]

<220>
<221>  misc_feature
<222>  (14)..(18)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa = Phe [F] or Tyr [Y]

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  GENES ENCODING A NOVEL TYPE OF LYSOPHOPHATIDYLCHOLINE 
       ACYLTRANSFERASES AND THEIR USE TO INCREASE TRIACYLGLYCEROL 
       PRODUCTION AND/OR MODIFY FATTY ACID COMPOSITION
<310>  U.S. Patent Publication No. 2008-0145867-A1
<311>  2007-06-15
<312>  2008-06-19
<313>  (1)..(15)

<400>  103

Arg Xaa Lys Tyr Tyr Xaa Xaa Trp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Gly Xaa Gly Xaa Xaa Gly 
            20                  


<210>  104
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa = Thr [T] or Phe [F]

<300>
<302>  GENES ENCODING A NOVEL TYPE OF LYSOPHOPHATIDYLCHOLINE 
       ACYLTRANSFERASES AND THEIR USE TO INCREASE TRIACYLGLYCEROL 
       PRODUCTION AND/OR MODIFY FATTY ACID COMPOSITION
<310>  U.S. Patent Publication No. 2008-0145867-A1
<311>  2007-06-15
<312>  2008-06-19
<313>  (1)..(15)

<400>  104

Ser Ala Xaa Trp His Gly Xaa Xaa Pro Gly Tyr Xaa Xaa Xaa Phe 
1               5                   10                  15  


<210>  105
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-acyl-sn-glycerol-3-phosphate acyltransferase motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa = Asp [D] or Arg [R]

<300>
<301>  Tal M. Lewin, Ping Wang, and Rosalind A. Coleman
<302>  Analysis of Amino Acid Motifs Diagnostic for the 
       sn-Glycerol-3-phosphate Acyltransferase Reaction
<303>  Biochemistry
<304>  38
<305>  18
<306>  57645771
<307>  1999-04-15
<313>  (1)..(7)

<300>
<301>  Atsushi Yamashita, Hiroki Nakanishia, Hiroshi Suzukia, Ryo 
       Kamataa, Ken Tanakaa, Keizo Wakua and Takayuki Sugiura
<302>  Topology of acyltransferase motifs and substrate specificity and 
       accessibility in 1-acyl-sn-glycero-3-phosphate acyltransferase 1
<303>  Biochimica et Biophysica Acta
<304>  1771
<305>  9
<306>  1202-1215
<307>  2007-07-17
<313>  (1)..(7)

<400>  105

Gly Xaa Xaa Phe Ile Xaa Arg 
1               5           


<210>  106
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-acyl-sn-glycerol-3-phosphate acyltransferase motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = Val [V] or Ile [I]

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = Pro [P] or Xaa

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa = Ile [I] or Val [V] or Leu [L]

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Ile [I] or Val [V]

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa =  Val [V] or Ile [I]

<300>
<301>  Atsushi Yamashita, Hiroki Nakanishia, Hiroshi Suzukia, Ryo 
       Kamataa, Ken Tanakaa, Keizo Wakua and Takayuki Sugiura
<302>  Topology of acyltransferase motifs and substrate specificity and 
       accessibility in 1-acyl-sn-glycero-3-phosphate acyltransferase 1
<303>  Biochimica et Biophysica Acta
<304>  1771
<305>  9
<306>  1202-1215
<307>  2007-07-17
<313>  (1)..(6)

<400>  106

Xaa Xaa Xaa Xaa Pro Xaa 
1               5       


<210>  107
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-acyl-sn-glycerol-3-phosphate acyltransferase motif

<300>
<301>  Atsushi Yamashita, Hiroki Nakanishia, Hiroshi Suzukia, Ryo 
       Kamataa, Ken Tanakaa, Keizo Wakua and Takayuki Sugiura
<302>  Topology of acyltransferase motifs and substrate specificity and 
       accessibility in 1-acyl-sn-glycero-3-phosphate acyltransferase 1
<303>  Biochimica et Biophysica Acta
<304>  1771
<305>  9
<306>  1202-1215
<307>  2007-07-17
<313>  (1)..(6)

<400>  107

Ile Val Pro Ile Val Met 
1               5       


<210>  108
<211>  1024
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(1024)
<223>  YlPex1p;  GenBank Accession No. CAG82178

<400>  108

Met Thr Ser Lys Ser Asp Tyr Ser Gly Lys Asp Lys Ile Glu Leu Asp 
1               5                   10                  15      


Pro Val Phe Ala Lys Ser Ile Asp Leu Leu Pro Asn Thr Gln Val Val 
            20                  25                  30          


Ile Asp Ile Gln Leu Asn Pro Lys Ile Ala His Thr Ile His Leu Glu 
        35                  40                  45              


Pro Val Thr Val Ala Asp Trp Glu Ile Val Glu Leu His Ala Ala Tyr 
    50                  55                  60                  


Leu Glu Ser Arg Met Ile Asn Gln Val Arg Ala Val Ser Pro Asn Gln 
65                  70                  75                  80  


Pro Val Thr Val Tyr Pro Ser Ser Thr Thr Ser Ala Thr Leu Lys Val 
                85                  90                  95      


Ile Arg Ile Glu Pro Asp Leu Gly Ala Ala Gly Phe Ala Lys Leu Ser 
            100                 105                 110         


Pro Asp Ser Glu Val Val Val Ala Pro Lys Gln Arg Lys Lys Glu Glu 
        115                 120                 125             


Lys Gln Val Lys Lys Arg Ser Gly Ser Ala Arg Ser Thr Gly Ser Gln 
    130                 135                 140                 


Lys Arg Lys Gly Gly Arg Gly Pro His Ala Leu Arg Arg Ala Ile Ser 
145                 150                 155                 160 


Glu Asp Phe Asp Gly His Leu Arg Leu Glu Val Ser Leu Asp Val Ser 
                165                 170                 175     


Gln Leu Pro Pro Glu Phe His Gln Leu Lys Asn Val Ser Ile Lys Val 
            180                 185                 190         


Ile Thr Pro Pro Asn Leu Ala Ser Pro Gln Gln Ala Ala Ser Ile Ala 
        195                 200                 205             


Val Glu Glu Lys Ser Glu Glu Ser Leu Ser Gln Asn Lys Pro Pro Ser 
    210                 215                 220                 


Ser Glu Pro Lys Val Glu Val Pro Pro Asp Ile Ile Asn Pro Ala Ser 
225                 230                 235                 240 


Glu Ile Val Ala Thr Leu Val Asn Asp Thr Thr Ser Pro Thr Gly His 
                245                 250                 255     


Ala Lys Leu Ser Tyr Ala Leu Ala Asp Ala Leu Gly Ile Pro Ser Ser 
            260                 265                 270         


Val Gly His Val Ile Arg Phe Glu Ser Ala Ser Lys Pro Leu Ser Gln 
        275                 280                 285             


Lys Pro Gly Ala Leu Val Ile His Arg Phe Ile Thr Lys Thr Val Gly 
    290                 295                 300                 


Ala Ala Glu Gln Lys Ser Leu Arg Leu Lys Gly Glu Lys Asn Ala Asp 
305                 310                 315                 320 


Asp Gly Val Ser Ala Asp Asp Gln Phe Ser Leu Leu Glu Glu Leu Lys 
                325                 330                 335     


Lys Leu Gln Met Leu Glu Gly Pro Ile Thr Asn Phe Gln Arg Leu Pro 
            340                 345                 350         


Pro Ile Pro Glu Leu Leu Pro Leu Gly Gly Val Ile Gly Leu Gln Asn 
        355                 360                 365             


Ser Glu Gly Trp Ile Gln Gly Gly Tyr Leu Gly Glu Glu Pro Ile Pro 
    370                 375                 380                 


Phe Val Ser Gly Ser Glu Ile Leu Arg Ser Glu Ser Ser Leu Ser Pro 
385                 390                 395                 400 


Ser Asn Ile Glu Ser Glu Asp Lys Arg Val Val Gly Leu Asp Asn Met 
                405                 410                 415     


Leu Asn Lys Ile Asn Glu Val Leu Ser Arg Asp Ser Ile Gly Cys Leu 
            420                 425                 430         


Val Tyr Gly Ser Arg Gly Ser Gly Lys Ser Ala Val Leu Asn His Ile 
        435                 440                 445             


Lys Lys Glu Cys Lys Val Ser His Thr His Thr Val Ser Ile Ala Cys 
    450                 455                 460                 


Gly Leu Ile Ala Gln Asp Arg Val Gln Ala Val Arg Glu Ile Leu Thr 
465                 470                 475                 480 


Lys Ala Phe Leu Glu Ala Ser Trp Phe Ser Pro Ser Val Leu Phe Leu 
                485                 490                 495     


Asp Asp Ile Asp Ala Leu Met Pro Ala Glu Val Glu His Ala Asp Ser 
            500                 505                 510         


Ser Arg Thr Arg Gln Leu Thr Gln Leu Phe Leu Glu Leu Ala Leu Pro 
        515                 520                 525             


Ile Met Lys Ser Arg His Val Ser Val Val Ala Ser Ala Gln Ala Lys 
    530                 535                 540                 


Glu Ser Leu His Met Asn Leu Val Thr Gly His Val Phe Glu Glu Leu 
545                 550                 555                 560 


Phe His Leu Lys Ser Pro Asp Lys Glu Ala Arg Leu Ala Ile Leu Ser 
                565                 570                 575     


Glu Ala Val Lys Leu Met Asp Gln Asn Val Ser Phe Ser Gln Asn Asp 
            580                 585                 590         


Val Leu Glu Ile Ala Ser Gln Val Asp Gly Tyr Leu Pro Gly Asp Leu 
        595                 600                 605             


Trp Thr Leu Ser Glu Arg Ala Gln His Glu Met Ala Leu Arg Gln Ile 
    610                 615                 620                 


Glu Ile Gly Leu Glu Asn Pro Ser Ile Gln Leu Ala Asp Phe Met Lys 
625                 630                 635                 640 


Ala Leu Glu Asp Phe Val Pro Ser Ser Leu Arg Gly Val Lys Leu Gln 
                645                 650                 655     


Lys Ser Asn Val Lys Trp Asn Asp Ile Gly Gly Leu Lys Glu Thr Lys 
            660                 665                 670         


Ala Val Leu Leu Glu Thr Leu Glu Trp Pro Thr Lys Tyr Ala Pro Ile 
        675                 680                 685             


Phe Ala Ser Cys Pro Leu Arg Leu Arg Ser Gly Leu Leu Leu Tyr Gly 
    690                 695                 700                 


Tyr Pro Gly Cys Gly Lys Thr Tyr Leu Ala Ser Ala Val Ala Ala Gln 
705                 710                 715                 720 


Cys Gly Leu Asn Phe Ile Ser Ile Lys Gly Pro Glu Ile Leu Asn Lys 
                725                 730                 735     


Tyr Ile Gly Ala Ser Glu Gln Ser Val Arg Glu Leu Phe Glu Arg Ala 
            740                 745                 750         


Gln Ala Ala Lys Pro Cys Ile Leu Phe Phe Asp Glu Phe Asp Ser Ile 
        755                 760                 765             


Ala Pro Lys Arg Gly His Asp Ser Thr Gly Val Thr Asp Arg Val Val 
    770                 775                 780                 


Asn Gln Met Leu Thr Gln Met Asp Gly Ala Glu Gly Leu Asp Gly Val 
785                 790                 795                 800 


Tyr Val Leu Ala Ala Thr Ser Arg Pro Asp Leu Ile Asp Pro Ala Leu 
                805                 810                 815     


Leu Arg Pro Gly Arg Leu Asp Lys Met Leu Ile Cys Asp Leu Pro Ser 
            820                 825                 830         


Tyr Glu Asp Arg Leu Asp Ile Leu Arg Ala Ile Val Asp Gly Lys Met 
        835                 840                 845             


His Leu Asp Gly Glu Val Glu Leu Glu Tyr Val Ala Ser Arg Thr Asp 
    850                 855                 860                 


Gly Phe Ser Gly Ala Asp Leu Gln Ala Val Met Phe Asn Ala Tyr Leu 
865                 870                 875                 880 


Glu Ala Ile His Glu Val Val Asp Val Ala Asp Asp Thr Ala Ala Asp 
                885                 890                 895     


Thr Pro Ala Leu Glu Asp Lys Arg Leu Glu Phe Phe Gln Thr Thr Leu 
            900                 905                 910         


Gly Asp Ala Lys Lys Asp Pro Ala Ala Val Gln Asn Glu Val Met Asn 
        915                 920                 925             


Ala Arg Ala Ala Val Ala Glu Lys Ala Arg Val Thr Ala Lys Leu Glu 
    930                 935                 940                 


Ala Leu Phe Lys Gly Met Ser Val Gly Val Asp Asn Asp Asp Asp Lys 
945                 950                 955                 960 


Pro Arg Lys Lys Ala Val Val Val Ile Lys Pro Gln His Met Asn Lys 
                965                 970                 975     


Ser Leu Asp Glu Thr Ser Pro Ser Ile Ser Lys Lys Glu Leu Leu Lys 
            980                 985                 990         


Leu Lys Gly Ile Tyr Ser Gln Phe  Val Ser Gly Arg Ser  Gly Asp Met 
        995                 1000                 1005             


Pro Pro  Gly Thr Ala Ser Thr  Asp Val Gly Gly Arg  Ala Thr Leu 
    1010                 1015                 1020             


Ala 
    


<210>  109
<211>  381
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(381)
<223>  YlPex2p; GenBank Accession No. CAG77647

<400>  109

Met Ser Ser Val Leu Arg Leu Phe Lys Ile Gly Ala Pro Val Pro Asn 
1               5                   10                  15      


Val Arg Val His Gln Leu Asp Ala Ser Leu Leu Asp Ala Glu Leu Val 
            20                  25                  30          


Asp Leu Leu Lys Asn Gln Leu Phe Lys Gly Phe Thr Asn Phe His Pro 
        35                  40                  45              


Glu Phe Arg Asp Lys Tyr Glu Ser Glu Leu Val Leu Ala Leu Lys Leu 
    50                  55                  60                  


Ile Leu Phe Lys Leu Thr Val Trp Asp His Ala Ile Thr Tyr Gly Gly 
65                  70                  75                  80  


Lys Leu Gln Asn Leu Lys Phe Ile Asp Ser Arg His Ser Ser Lys Leu 
                85                  90                  95      


Gln Ile Gln Pro Ser Val Ile Gln Lys Leu Gly Tyr Gly Ile Leu Val 
            100                 105                 110         


Val Gly Gly Gly Tyr Leu Trp Ser Lys Ile Glu Gly Tyr Leu Leu Ala 
        115                 120                 125             


Arg Ser Glu Asp Asp Val Ala Thr Asp Gly Thr Ser Val Arg Gly Ala 
    130                 135                 140                 


Ser Ala Ala Arg Gly Ala Leu Lys Val Ala Asn Phe Ala Ser Leu Leu 
145                 150                 155                 160 


Tyr Ser Ala Ala Thr Leu Gly Asn Phe Val Ala Phe Leu Tyr Thr Gly 
                165                 170                 175     


Arg Tyr Ala Thr Val Ile Met Arg Leu Leu Arg Ile Arg Leu Val Pro 
            180                 185                 190         


Ser Gln Arg Thr Ser Ser Arg Gln Val Ser Tyr Glu Phe Gln Asn Arg 
        195                 200                 205             


Gln Leu Val Trp Asn Ala Phe Thr Glu Phe Leu Ile Phe Ile Leu Pro 
    210                 215                 220                 


Leu Leu Gln Leu Pro Lys Leu Lys Arg Arg Ile Glu Arg Lys Leu Gln 
225                 230                 235                 240 


Ser Leu Asn Val Thr Arg Val Gly Asn Val Glu Glu Ala Ser Glu Gly 
                245                 250                 255     


Glu Leu Ala His Leu Pro Gln Lys Thr Cys Ala Ile Cys Phe Arg Asp 
            260                 265                 270         


Glu Glu Glu Gln Glu Gly Gly Gly Gly Ala Ser His Tyr Ser Thr Asp 
        275                 280                 285             


Val Thr Asn Pro Tyr Gln Ala Asp Cys Gly His Val Tyr Cys Tyr Val 
    290                 295                 300                 


Cys Leu Val Thr Lys Leu Ala Gln Gly Asp Gly Asp Gly Trp Asn Cys 
305                 310                 315                 320 


Tyr Arg Cys Ala Lys Gln Val Gln Lys Met Lys Pro Trp Val Asp Val 
                325                 330                 335     


Asp Glu Ala Ala Val Val Gly Ala Ala Glu Met His Glu Lys Val Asp 
            340                 345                 350         


Val Ile Glu His Ala Glu Asp Asn Glu Gln Glu Glu Glu Glu Phe Asp 
        355                 360                 365             


Asp Asp Asp Glu Asp Ser Asn Phe Gln Leu Met Lys Asp 
    370                 375                 380     


<210>  110
<211>  431
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(431)
<223>  YlPex3p; GenBank Accession No. CAG78565

<400>  110

Met Asp Phe Phe Arg Arg His Gln Lys Lys Val Leu Ala Leu Val Gly 
1               5                   10                  15      


Val Ala Leu Ser Ser Tyr Leu Phe Ile Asp Tyr Val Lys Lys Lys Phe 
            20                  25                  30          


Phe Glu Ile Gln Gly Arg Leu Ser Ser Glu Arg Thr Ala Lys Gln Asn 
        35                  40                  45              


Leu Arg Arg Arg Phe Glu Gln Asn Gln Gln Asp Ala Asp Phe Thr Ile 
    50                  55                  60                  


Met Ala Leu Leu Ser Ser Leu Thr Thr Pro Val Met Glu Arg Tyr Pro 
65                  70                  75                  80  


Val Asp Gln Ile Lys Ala Glu Leu Gln Ser Lys Arg Arg Pro Thr Asp 
                85                  90                  95      


Arg Val Leu Ala Leu Glu Ser Ser Thr Ser Ser Ser Ala Thr Ala Gln 
            100                 105                 110         


Thr Val Pro Thr Met Thr Ser Gly Ala Thr Glu Glu Gly Glu Lys Ser 
        115                 120                 125             


Lys Thr Gln Leu Trp Gln Asp Leu Lys Arg Thr Thr Ile Ser Arg Ala 
    130                 135                 140                 


Phe Ser Leu Val Tyr Ala Asp Ala Leu Leu Ile Phe Phe Thr Arg Leu 
145                 150                 155                 160 


Gln Leu Asn Ile Leu Gly Arg Arg Asn Tyr Val Asn Ser Val Val Ala 
                165                 170                 175     


Leu Ala Gln Gln Gly Arg Glu Gly Asn Ala Glu Gly Arg Val Ala Pro 
            180                 185                 190         


Ser Phe Gly Asp Leu Ala Asp Met Gly Tyr Phe Gly Asp Leu Ser Gly 
        195                 200                 205             


Ser Ser Ser Phe Gly Glu Thr Ile Val Asp Pro Asp Leu Asp Glu Gln 
    210                 215                 220                 


Tyr Leu Thr Phe Ser Trp Trp Leu Leu Asn Glu Gly Trp Val Ser Leu 
225                 230                 235                 240 


Ser Glu Arg Val Glu Glu Ala Val Arg Arg Val Trp Asp Pro Val Ser 
                245                 250                 255     


Pro Lys Ala Glu Leu Gly Phe Asp Glu Leu Ser Glu Leu Ile Gly Arg 
            260                 265                 270         


Thr Gln Met Leu Ile Asp Arg Pro Leu Asn Pro Ser Ser Pro Leu Asn 
        275                 280                 285             


Phe Leu Ser Gln Leu Leu Pro Pro Arg Glu Gln Glu Glu Tyr Val Leu 
    290                 295                 300                 


Ala Gln Asn Pro Ser Asp Thr Ala Ala Pro Ile Val Gly Pro Thr Leu 
305                 310                 315                 320 


Arg Arg Leu Leu Asp Glu Thr Ala Asp Phe Ile Glu Ser Pro Asn Ala 
                325                 330                 335     


Ala Glu Val Ile Glu Arg Leu Val His Ser Gly Leu Ser Val Phe Met 
            340                 345                 350         


Asp Lys Leu Ala Val Thr Phe Gly Ala Thr Pro Ala Asp Ser Gly Ser 
        355                 360                 365             


Pro Tyr Pro Val Val Leu Pro Thr Ala Lys Val Lys Leu Pro Ser Ile 
    370                 375                 380                 


Leu Ala Asn Met Ala Arg Gln Ala Gly Gly Met Ala Gln Gly Ser Pro 
385                 390                 395                 400 


Gly Val Glu Asn Glu Tyr Ile Asp Val Met Asn Gln Val Gln Glu Leu 
                405                 410                 415     


Thr Ser Phe Ser Ala Val Val Tyr Ser Ser Phe Asp Trp Ala Leu 
            420                 425                 430     


<210>  111
<211>  395
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(395)
<223>  YlPex3Bp; GenBank Accession No. CAG83356

<400>  111

Met Leu Gln Ser Leu Asn Arg Asn Lys Lys Arg Leu Ala Val Ser Thr 
1               5                   10                  15      


Gly Leu Ile Ala Val Ala Tyr Val Val Ile Ser Tyr Thr Thr Lys Arg 
            20                  25                  30          


Leu Ile Glu Lys Gln Glu Gln Lys Leu Glu Glu Glu Arg Ala Lys Glu 
        35                  40                  45              


Arg Leu Lys Gln Leu Phe Ala Gln Thr Gln Asn Glu Ala Ala Phe His 
    50                  55                  60                  


Thr Ala Ser Val Leu Pro Gln Leu Cys Glu Gln Ile Met Glu Phe Val 
65                  70                  75                  80  


Ala Val Glu Lys Ile Ala Glu Gln Leu Gln Asn Met Arg Ala Glu Lys 
                85                  90                  95      


Arg Lys Lys Gln Asn Met Asp Asp Asp Lys His Ser Val Leu Ser Leu 
            100                 105                 110         


Gly Thr Glu Thr Thr Ala Ser Met Ala Asp Gly Gln Lys Met Ser Lys 
        115                 120                 125             


Ile Gln Leu Trp Asp Glu Leu Lys Ile Glu Ser Leu Thr Arg Ile Val 
    130                 135                 140                 


Thr Leu Ile Tyr Cys Val Ser Leu Leu Asn Tyr Leu Ile Arg Leu Gln 
145                 150                 155                 160 


Thr Asn Ile Val Gly Arg Lys Arg Tyr Gln Asn Glu Ala Gly Pro Ala 
                165                 170                 175     


Gly Ala Thr Tyr Asp Met Ser Leu Glu Gln Cys Tyr Thr Trp Leu Leu 
            180                 185                 190         


Thr Arg Gly Trp Lys Ser Val Val Asp Asn Val Arg Arg Ser Val Gln 
        195                 200                 205             


Gln Val Phe Thr Gly Val Asn Pro Arg Gln Asn Leu Ser Leu Asp Glu 
    210                 215                 220                 


Phe Ala Thr Leu Leu Lys Arg Val Gln Thr Leu Val Asn Ser Pro Pro 
225                 230                 235                 240 


Tyr Ser Thr Thr Pro Asn Thr Phe Leu Thr Ser Leu Leu Pro Pro Arg 
                245                 250                 255     


Glu Leu Glu Gln Leu Arg Leu Glu Lys Glu Lys Gln Ser Leu Ser Pro 
            260                 265                 270         


Asn Tyr Thr Tyr Gly Ser Pro Leu Lys Asp Leu Val Phe Glu Ser Ala 
        275                 280                 285             


Gln His Ile Gln Ser Pro Gln Gly Met Ser Ser Phe Arg Ala Ile Ile 
    290                 295                 300                 


Asp Gln Ser Phe Lys Val Phe Leu Glu Lys Val Asn Glu Ser Gln Tyr 
305                 310                 315                 320 


Val Asn Pro Pro Ser Thr Gly Gly Lys Arg Ile Ala Val Gly Ala Leu 
                325                 330                 335     


Gln Pro Pro Ile Ile Ser Gly Gly Pro Lys Lys Val Lys Leu Ala Ser 
            340                 345                 350         


Leu Leu Ser Val Ala Thr Arg Gln Ser Ser Val Ile Ser His Ala Gln 
        355                 360                 365             


Pro Asn Pro Tyr Val Asp Ala Ile Asn Ser Val Ala Glu Tyr Asn Gly 
    370                 375                 380                 


Leu Cys Ala Val Ile Tyr Ser Ser Phe Glu Gln 
385                 390                 395 


<210>  112
<211>  153
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(153)
<223>  YlPex4p; GenBank Accession No. CAG79130

<400>  112

Met Ala Ser Gln Lys Arg Leu Ile Lys Glu Leu Ala Ala Tyr Lys Lys 
1               5                   10                  15      


Asp Pro Asn Pro Cys Leu Ala Ser Leu Thr Ala Asp Gly Asp Ser Leu 
            20                  25                  30          


Tyr Lys Trp Thr Ala Val Met Arg Gly Thr Glu Gly Thr Ala Tyr Glu 
        35                  40                  45              


Asn Gly Leu Trp Gln Val Glu Ile Asn Ile Pro Glu Asn Tyr Pro Leu 
    50                  55                  60                  


Gln Pro Pro Thr Met Phe Phe Arg Thr Lys Ile Cys His Pro Asn Ile 
65                  70                  75                  80  


His Phe Glu Thr Gly Glu Val Cys Ile Asp Val Leu Lys Thr Gln Trp 
                85                  90                  95      


Ser Pro Ala Trp Thr Ile Ser Ser Ala Cys Thr Ala Val Ser Ala Met 
            100                 105                 110         


Leu Ser Leu Pro Glu Pro Asp Ser Pro Leu Asn Ile Asp Ala Ala Asn 
        115                 120                 125             


Leu Val Arg Cys Gly Asp Glu Ser Ala Met Glu Gly Leu Val Arg Tyr 
    130                 135                 140                 


Tyr Val Asn Lys Tyr Ala Ser Gly Asn 
145                 150             


<210>  113
<211>  598
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(598)
<223>  YlPex5p; GenBank Accession No. CAG78803

<400>  113

Met Ser Phe Met Arg Gly Gly Ser Glu Cys Ser Thr Gly Arg Asn Pro 
1               5                   10                  15      


Leu Ser Gln Phe Thr Lys His Thr Ala Glu Asp Arg Ser Leu Gln His 
            20                  25                  30          


Asp Arg Val Ala Gly Pro Ser Gly Gly Arg Val Gly Gly Met Arg Ser 
        35                  40                  45              


Asn Thr Gly Glu Met Ser Gln Gln Asp Arg Glu Met Met Ala Arg Phe 
    50                  55                  60                  


Gly Ala Ala Gly Pro Glu Gln Ser Ser Phe Asn Tyr Glu Gln Met Arg 
65                  70                  75                  80  


His Glu Leu His Asn Met Gly Ala Gln Gly Gly Gln Ile Pro Gln Val 
                85                  90                  95      


Pro Ser Gln Gln Gly Ala Ala Asn Gly Gly Gln Trp Ala Arg Asp Phe 
            100                 105                 110         


Gly Gly Gln Gln Thr Ala Pro Gly Ala Ala Pro Gln Asp Ala Lys Asn 
        115                 120                 125             


Trp Asn Ala Glu Phe Gln Arg Gly Gly Ser Pro Ala Glu Ala Met Gln 
    130                 135                 140                 


Gln Gln Gly Pro Gly Pro Met Gln Gly Gly Met Gly Met Gly Gly Met 
145                 150                 155                 160 


Pro Met Tyr Gly Met Ala Arg Pro Met Tyr Ser Gly Met Ser Ala Asn 
                165                 170                 175     


Met Ala Pro Gln Phe Gln Pro Gln Gln Ala Asn Ala Arg Val Val Glu 
            180                 185                 190         


Leu Asp Glu Gln Asn Trp Glu Glu Gln Phe Lys Gln Met Asp Ser Ala 
        195                 200                 205             


Val Gly Lys Gly Lys Glu Val Glu Glu Gln Thr Ala Glu Thr Ala Thr 
    210                 215                 220                 


Ala Thr Glu Thr Val Thr Glu Thr Glu Thr Thr Thr Glu Asp Lys Pro 
225                 230                 235                 240 


Met Asp Ile Lys Asn Met Asp Phe Glu Asn Ile Trp Lys Asn Leu Gln 
                245                 250                 255     


Val Asn Val Leu Asp Asn Met Asp Glu Trp Leu Glu Glu Thr Asn Ser 
            260                 265                 270         


Pro Ala Trp Glu Arg Asp Phe His Glu Tyr Thr His Asn Arg Pro Glu 
        275                 280                 285             


Phe Ala Asp Tyr Gln Phe Glu Glu Asn Asn Gln Phe Met Glu His Pro 
    290                 295                 300                 


Asp Pro Phe Lys Ile Gly Val Glu Leu Met Glu Thr Gly Gly Arg Leu 
305                 310                 315                 320 


Ser Glu Ala Ala Leu Ala Phe Glu Ala Ala Val Gln Lys Asn Thr Glu 
                325                 330                 335     


His Ala Glu Ala Trp Gly Arg Leu Gly Ala Cys Gln Ala Gln Asn Glu 
            340                 345                 350         


Lys Glu Asp Pro Ala Ile Arg Ala Leu Glu Arg Cys Ile Lys Leu Glu 
        355                 360                 365             


Pro Gly Asn Leu Ser Ala Leu Met Asn Leu Ser Val Ser Tyr Thr Asn 
    370                 375                 380                 


Glu Gly Tyr Glu Asn Ala Ala Tyr Ala Thr Leu Glu Arg Trp Leu Ala 
385                 390                 395                 400 


Thr Lys Tyr Pro Glu Val Val Asp Gln Ala Arg Asn Gln Glu Pro Arg 
                405                 410                 415     


Leu Gly Asn Glu Asp Lys Phe Gln Leu His Ser Arg Val Thr Glu Leu 
            420                 425                 430         


Phe Ile Arg Ala Ala Gln Leu Ser Pro Asp Gly Ala Asn Ile Asp Ala 
        435                 440                 445             


Asp Val Gln Val Gly Leu Gly Val Leu Phe Tyr Gly Asn Glu Glu Tyr 
    450                 455                 460                 


Asp Lys Ala Ile Asp Cys Phe Asn Ala Ala Ile Ala Val Arg Pro Asp 
465                 470                 475                 480 


Asp Ala Leu Leu Trp Asn Arg Leu Gly Ala Thr Leu Ala Asn Ser His 
                485                 490                 495     


Arg Ser Glu Glu Ala Ile Asp Ala Tyr Tyr Lys Ala Leu Glu Leu Arg 
            500                 505                 510         


Pro Ser Phe Val Arg Ala Arg Tyr Asn Leu Gly Val Ser Cys Ile Asn 
        515                 520                 525             


Ile Gly Cys Tyr Lys Glu Ala Ala Gln Tyr Leu Leu Gly Ala Leu Ser 
    530                 535                 540                 


Met His Lys Val Glu Gly Val Gln Asp Asp Val Leu Ala Asn Gln Ser 
545                 550                 555                 560 


Thr Asn Leu Tyr Asp Thr Leu Lys Arg Val Phe Leu Gly Met Asp Arg 
                565                 570                 575     


Arg Asp Leu Val Ala Lys Val Gly Asn Gly Met Asp Val Asn Gln Phe 
            580                 585                 590         


Arg Asn Glu Phe Glu Phe 
        595             


<210>  114
<211>  1024
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(1024)
<223>  YlPex6p; GenBank Accession No. CAG82306

<400>  114

Met Pro Ser Ile Ser His Lys Pro Ile Thr Ala Lys Leu Val Ala Ala 
1               5                   10                  15      


Pro Asp Ala Thr Lys Leu Glu Leu Ser Ser Tyr Leu Tyr Gln Gln Leu 
            20                  25                  30          


Phe Ser Asp Lys Pro Ala Glu Pro Tyr Val Ala Phe Glu Ala Pro Gly 
        35                  40                  45              


Ile Lys Trp Ala Leu Tyr Pro Ala Ser Glu Asp Arg Ser Leu Pro Gln 
    50                  55                  60                  


Tyr Thr Cys Lys Ala Asp Ile Arg His Val Ala Gly Ser Leu Lys Lys 
65                  70                  75                  80  


Phe Met Pro Val Val Leu Lys Arg Val Asn Pro Val Thr Ile Glu His 
                85                  90                  95      


Ala Ile Val Thr Val Pro Ala Ser Gln Tyr Glu Thr Leu Asn Thr Pro 
            100                 105                 110         


Glu Gln Val Leu Lys Ala Leu Glu Pro Gln Leu Asp Lys Asp Arg Pro 
        115                 120                 125             


Val Ile Arg Gln Gly Asp Val Leu Leu Asn Gly Cys Arg Val Arg Leu 
    130                 135                 140                 


Cys Glu Pro Val Asn Gln Gly Lys Val Val Lys Gly Thr Thr Lys Leu 
145                 150                 155                 160 


Thr Val Ala Lys Glu Gln Glu Thr Ile Gln Pro Ala Asp Glu Ala Ala 
                165                 170                 175     


Asp Val Ala Phe Asp Ile Ala Glu Phe Leu Asp Phe Asp Thr Ser Val 
            180                 185                 190         


Ala Lys Thr Arg Glu Ser Thr Asn Leu Gln Val Ala Pro Leu Glu Gly 
        195                 200                 205             


Ala Ile Pro Thr Pro Leu Ser Asp Arg Phe Asp Asp Cys Glu Ser Arg 
    210                 215                 220                 


Gly Phe Val Lys Ser Glu Thr Met Ser Lys Leu Gly Val Phe Ser Gly 
225                 230                 235                 240 


Asp Ile Val Ser Ile Lys Thr Lys Asn Gly Ala Glu Arg Val Leu Arg 
                245                 250                 255     


Leu Phe Ala Tyr Pro Glu Pro Asn Thr Val Lys Tyr Asp Val Val Tyr 
            260                 265                 270         


Val Ser Pro Ile Leu Tyr His Asn Ile Gly Asp Lys Glu Ile Glu Val 
        275                 280                 285             


Thr Pro Asn Gly Glu Thr His Lys Ser Val Gly Glu Ala Leu Asp Ser 
    290                 295                 300                 


Val Leu Glu Ala Ala Glu Glu Val Lys Leu Ala Arg Val Leu Gly Pro 
305                 310                 315                 320 


Thr Thr Thr Asp Arg Thr Phe Gln Thr Ala Tyr His Ala Gly Leu Gln 
                325                 330                 335     


Ala Tyr Phe Lys Pro Val Lys Arg Ala Val Arg Val Gly Asp Leu Ile 
            340                 345                 350         


Pro Ile Pro Phe Asp Ser Ile Leu Ala Arg Thr Ile Gly Glu Asp Pro 
        355                 360                 365             


Glu Met Ser His Ile Pro Leu Glu Ala Leu Ala Val Lys Pro Asp Ser 
    370                 375                 380                 


Val Ala Trp Phe Gln Val Thr Ser Leu Asn Gly Ser Glu Asp Pro Ala 
385                 390                 395                 400 


Ser Lys Gln Tyr Leu Val Asp Ser Ser Gln Thr Lys Leu Ile Glu Gly 
                405                 410                 415     


Gly Thr Thr Ser Ser Ala Val Ile Pro Thr Ser Val Pro Trp Arg Glu 
            420                 425                 430         


Tyr Leu Gly Leu Asp Thr Leu Pro Lys Phe Gly Ser Glu Phe Ala Tyr 
        435                 440                 445             


Ala Asp Lys Ile Arg Asn Leu Val Gln Ile Ser Thr Ser Ala Leu Ser 
    450                 455                 460                 


His Ala Lys Leu Asn Thr Ser Val Leu Leu His Ser Ala Lys Arg Gly 
465                 470                 475                 480 


Val Gly Lys Ser Thr Val Leu Arg Ser Val Ala Ala Gln Cys Gly Ile 
                485                 490                 495     


Ser Val Phe Glu Ile Ser Cys Phe Gly Leu Ile Gly Asp Asn Glu Ala 
            500                 505                 510         


Gln Thr Leu Gly Thr Leu Arg Ala Lys Leu Asp Arg Ala Tyr Gly Cys 
        515                 520                 525             


Ser Pro Cys Val Val Val Leu Gln His Leu Glu Ser Ile Ala Lys Lys 
    530                 535                 540                 


Ser Asp Gln Asp Gly Lys Asp Glu Gly Ile Val Ser Lys Leu Val Asp 
545                 550                 555                 560 


Val Leu Ala Asp Tyr Ser Gly His Gly Val Leu Leu Ala Ala Thr Ser 
                565                 570                 575     


Asn Asp Pro Asp Lys Ile Ser Glu Ala Ile Arg Ser Arg Phe Gln Phe 
            580                 585                 590         


Glu Ile Glu Ile Gly Val Pro Ser Glu Pro Gln Arg Arg Gln Ile Phe 
        595                 600                 605             


Ser His Leu Thr Lys Ser Gly Pro Gly Gly Asp Ser Ile Arg Asn Ala 
    610                 615                 620                 


Pro Ile Ser Leu Arg Ser Asp Val Ser Val Glu Asn Leu Ala Leu Gln 
625                 630                 635                 640 


Ser Ala Gly Leu Thr Pro Pro Asp Leu Thr Ala Ile Val Gln Thr Thr 
                645                 650                 655     


Arg Leu Arg Ala Ile Asp Arg Leu Asn Lys Leu Thr Lys Asp Ser Asp 
            660                 665                 670         


Thr Thr Leu Asp Asp Leu Leu Thr Leu Ser His Gly Thr Leu Gln Leu 
        675                 680                 685             


Thr Pro Ser Asp Phe Asp Asp Ala Ile Ala Asp Ala Arg Gln Lys Tyr 
    690                 695                 700                 


Ser Asp Ser Ile Gly Ala Pro Arg Ile Pro Asn Val Gly Trp Asp Asp 
705                 710                 715                 720 


Val Gly Gly Met Glu Gly Val Lys Lys Asp Ile Leu Asp Thr Ile Glu 
                725                 730                 735     


Thr Pro Leu Lys Tyr Pro His Trp Phe Ser Asp Gly Val Lys Lys Arg 
            740                 745                 750         


Ser Gly Ile Leu Phe Tyr Gly Pro Pro Gly Thr Gly Lys Thr Leu Leu 
        755                 760                 765             


Ala Lys Ala Ile Ala Thr Thr Phe Ser Leu Asn Phe Phe Ser Val Lys 
    770                 775                 780                 


Gly Pro Glu Leu Leu Asn Met Tyr Ile Gly Glu Ser Glu Ala Asn Val 
785                 790                 795                 800 


Arg Arg Val Phe Gln Lys Ala Arg Asp Ala Lys Pro Cys Val Val Phe 
                805                 810                 815     


Phe Asp Glu Leu Asp Ser Val Ala Pro Gln Arg Gly Asn Gln Gly Asp 
            820                 825                 830         


Ser Gly Gly Val Met Asp Arg Ile Val Ser Gln Leu Leu Ala Glu Leu 
        835                 840                 845             


Asp Gly Met Ser Thr Ala Gly Gly Glu Gly Val Phe Val Val Gly Ala 
    850                 855                 860                 


Thr Asn Arg Pro Asp Leu Leu Asp Glu Ala Leu Leu Arg Pro Gly Arg 
865                 870                 875                 880 


Phe Asp Lys Met Leu Tyr Leu Gly Ile Ser Asp Thr His Glu Lys Gln 
                885                 890                 895     


Gln Thr Ile Met Glu Ala Leu Thr Arg Lys Phe Arg Leu Ala Ala Asp 
            900                 905                 910         


Val Ser Leu Glu Ala Ile Ser Lys Arg Cys Pro Phe Thr Phe Thr Gly 
        915                 920                 925             


Ala Asp Phe Tyr Ala Leu Cys Ser Asp Ala Met Leu Asn Ala Met Thr 
    930                 935                 940                 


Arg Thr Ala Asn Glu Val Asp Ala Lys Ile Lys Leu Leu Asn Lys Asn 
945                 950                 955                 960 


Arg Glu Glu Ala Gly Glu Glu Pro Val Ser Ile Arg Trp Trp Phe Asp 
                965                 970                 975     


His Glu Ala Thr Lys Ser Asp Ile Glu Val Glu Val Ala Gln Gln Asp 
            980                 985                 990         


Phe Glu Lys Ala Lys Asp Glu Leu  Ser Pro Ser Val Ser  Ala Glu Glu 
        995                 1000                 1005             


Leu Gln  His Tyr Leu Lys Leu  Arg Gln Gln Phe Glu  Gly Gly Lys 
    1010                 1015                 1020             


Lys 
    


<210>  115
<211>  356
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(356)
<223>  YlPex7p; GenBank Accession No. CAG78389

<400>  115

Met Leu Gly Phe Lys Thr Gln Gly Phe Asn Gly Tyr Ala Ala Asn Tyr 
1               5                   10                  15      


Ser Pro Phe Phe Asn Asp Lys Ile Ala Val Gly Thr Ala Ala Asn Tyr 
            20                  25                  30          


Gly Leu Val Gly Asn Gly Lys Leu Phe Ile Leu Gly Ile Ser Pro Glu 
        35                  40                  45              


Gly Arg Met Val Cys Glu Gly Gln Phe Asp Thr Gln Asp Gly Ile Phe 
    50                  55                  60                  


Asp Val Ala Trp Ser Glu Gln His Glu Asn His Val Ala Thr Ala Cys 
65                  70                  75                  80  


Gly Asp Gly Ser Val Lys Leu Phe Asp Ile Lys Ala Gly Ala Phe Pro 
                85                  90                  95      


Leu Val Ser Phe Lys Glu His Thr Arg Glu Val Phe Ser Val Asn Trp 
            100                 105                 110         


Asn Met Ala Asn Lys Ala Leu Phe Cys Thr Ser Ser Trp Asp Ser Thr 
        115                 120                 125             


Ile Lys Ile Trp Thr Pro Glu Arg Thr Asn Ser Ile Met Thr Leu Gly 
    130                 135                 140                 


Gln Pro Ala Pro Ala Gln Gly Thr Asn Ala Ser Ala His Ile Gly Arg 
145                 150                 155                 160 


Gln Thr Ala Pro Asn Gln Ala Ala Ala Gln Glu Cys Ile Tyr Ser Ala 
                165                 170                 175     


Lys Phe Ser Pro His Thr Asp Ser Ile Ile Ala Ser Ala His Ser Thr 
            180                 185                 190         


Gly Met Val Lys Val Trp Asp Thr Arg Ala Pro Gln Pro Leu Gln Gln 
        195                 200                 205             


Gln Phe Ser Thr Gln Gln Thr Glu Ser Gly Gly Pro Pro Glu Val Leu 
    210                 215                 220                 


Ser Leu Asp Trp Asn Lys Tyr Arg Pro Thr Val Ile Ala Thr Gly Gly 
225                 230                 235                 240 


Val Asp Arg Ser Val Gln Val Tyr Asp Ile Arg Met Thr Gln Pro Ala 
                245                 250                 255     


Ala Asn Gln Pro Val Gln Pro Leu Ser Leu Ile Leu Gly His Arg Leu 
            260                 265                 270         


Pro Val Arg Gly Val Ser Trp Ser Pro His His Ala Asp Leu Leu Leu 
        275                 280                 285             


Ser Cys Ser Tyr Asp Met Thr Ala Arg Val Trp Arg Asp Ala Ser Thr 
    290                 295                 300                 


Gly Gly Asn Tyr Leu Ala Arg Gln Arg Gly Gly Thr Glu Val Lys Cys 
305                 310                 315                 320 


Met Asp Arg His Thr Glu Phe Val Ile Gly Gly Asp Trp Ser Leu Trp 
                325                 330                 335     


Gly Asp Pro Gly Trp Ile Thr Thr Val Gly Trp Asp Gln Met Val Tyr 
            340                 345                 350         


Val Trp His Ala 
        355     


<210>  116
<211>  671
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(671)
<223>  YlPex8p; GenBank Accession No. CAG80447

<400>  116

Met Asn Lys Tyr Leu Val Pro Pro Pro Gln Ala Asn Arg Thr Val Thr 
1               5                   10                  15      


Asn Leu Asp Leu Leu Ile Asn Asn Leu Arg Gly Ser Ser Thr Pro Gly 
            20                  25                  30          


Ala Ala Glu Val Asp Thr Arg Asp Ile Leu Gln Arg Ile Val Phe Ile 
        35                  40                  45              


Leu Pro Thr Ile Lys Asn Pro Leu Asn Leu Asp Leu Val Ile Lys Glu 
    50                  55                  60                  


Ile Ile Asn Ser Pro Arg Leu Leu Pro Pro Leu Ile Asp Leu His Asp 
65                  70                  75                  80  


Tyr Gln Gln Leu Thr Asp Ala Phe Arg Ala Thr Ile Lys Arg Lys Ala 
                85                  90                  95      


Leu Val Thr Asp Pro Thr Ile Ser Phe Glu Ala Trp Leu Glu Thr Cys 
            100                 105                 110         


Phe Gln Val Ile Thr Arg Phe Ala Gly Pro Gly Trp Lys Lys Leu Pro 
        115                 120                 125             


Leu Leu Ala Gly Leu Ile Leu Ala Asp Tyr Asp Ile Ser Ala Asp Gly 
    130                 135                 140                 


Pro Thr Leu Glu Arg Lys Pro Gly Phe Pro Ser Lys Leu Lys His Leu 
145                 150                 155                 160 


Leu Lys Arg Glu Phe Val Thr Thr Phe Asp Gln Cys Leu Ser Ile Asp 
                165                 170                 175     


Thr Arg Asn Arg Ser Asp Ala Thr Lys Trp Val Pro Val Leu Ala Cys 
            180                 185                 190         


Ile Ser Ile Ala Gln Val Tyr Ser Leu Leu Gly Asp Val Ala Ile Asn 
        195                 200                 205             


Tyr Arg Arg Phe Leu Gln Val Gly Leu Asp Leu Ile Phe Ser Asn Tyr 
    210                 215                 220                 


Gly Leu Glu Met Gly Thr Ala Leu Ala Arg Leu His Ala Glu Ser Gly 
225                 230                 235                 240 


Gly Asp Ala Thr Thr Ala Gly Gly Leu Ile Gly Lys Lys Leu Lys Glu 
                245                 250                 255     


Pro Val Val Ala Leu Leu Asn Thr Phe Ala His Ile Ala Ser Ser Cys 
            260                 265                 270         


Ile Val His Val Asp Ile Asp Tyr Ile Asp Arg Ile Gln Asn Lys Ile 
        275                 280                 285             


Ile Leu Val Cys Glu Asn Gln Ala Glu Thr Trp Arg Ile Leu Thr Ile 
    290                 295                 300                 


Glu Ser Pro Thr Val Met His His Gln Glu Ser Val Gln Tyr Leu Lys 
305                 310                 315                 320 


Trp Glu Leu Phe Thr Leu Cys Ile Ile Met Gln Gly Ile Ala Asn Met 
                325                 330                 335     


Leu Leu Thr Gln Lys Met Asn Gln Phe Met Tyr Leu Gln Leu Ala Tyr 
            340                 345                 350         


Lys Gln Leu Gln Ala Leu His Ser Ile Tyr Phe Ile Val Asp Gln Met 
        355                 360                 365             


Gly Ser Gln Phe Ala Ala Tyr Asp Tyr Val Phe Phe Ser Ala Ile Asp 
    370                 375                 380                 


Val Leu Leu Ser Glu Tyr Ala Pro Tyr Ile Lys Asn Arg Gly Thr Ile 
385                 390                 395                 400 


Pro Pro Asn Lys Glu Phe Val Ala Glu Arg Leu Ala Ala Asn Leu Ala 
                405                 410                 415     


Gly Thr Ser Asn Val Gly Ser His Leu Pro Ile Asp Arg Ser Arg Val 
            420                 425                 430         


Leu Phe Ala Leu Asn Tyr Tyr Glu Gln Leu Val Thr Val Cys His Asp 
        435                 440                 445             


Ser Cys Val Glu Thr Ile Ile Tyr Pro Met Ala Arg Ser Phe Leu Tyr 
    450                 455                 460                 


Pro Thr Ser Asp Ile Gln Gln Leu Lys Pro Leu Val Glu Ala Ala His 
465                 470                 475                 480 


Ser Val Ile Leu Ala Gly Leu Ala Val Pro Thr Asn Ala Val Val Asn 
                485                 490                 495     


Ala Lys Leu Ile Pro Glu Tyr Met Gly Gly Val Leu Pro Leu Phe Pro 
            500                 505                 510         


Gly Val Phe Ser Trp Asn Gln Phe Val Leu Ala Ile Gln Ser Ile Val 
        515                 520                 525             


Asn Thr Val Ser Pro Pro Ser Glu Val Phe Lys Thr Asn Gln Lys Leu 
    530                 535                 540                 


Phe Arg Leu Val Leu Asp Ser Leu Met Lys Lys Cys Arg Asp Thr Pro 
545                 550                 555                 560 


Val Gly Ile Pro Val Pro His Ser Val Thr Val Ser Gln Glu Gln Glu 
                565                 570                 575     


Asp Ile Pro Pro Thr Gln Arg Ala Val Val Met Leu Ala Leu Ile Asn 
            580                 585                 590         


Ser Leu Pro Tyr Val Asp Ile Arg Ser Phe Glu Leu Trp Leu Gln Glu 
        595                 600                 605             


Thr Trp Asn Met Ile Glu Ala Thr Pro Met Leu Ala Glu Asn Ala Pro 
    610                 615                 620                 


Asn Lys Glu Leu Ala His Ala Glu His Glu Phe Leu Val Leu Glu Met 
625                 630                 635                 640 


Trp Lys Met Ile Ser Gly Asn Ile Asp Gln Arg Leu Asn Asp Val Ala 
                645                 650                 655     


Ile Arg Trp Trp Tyr Lys Lys Asn Ala Arg Val His Gly Thr Leu 
            660                 665                 670     


<210>  117
<211>  377
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(377)
<223>  YlPex10p; GenBank Accession No. CAG81606

<400>  117

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser 
            340                 345                 350         


Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val 
        355                 360                 365             


Arg Glu Gln Asn Leu Leu Pro Ile Arg 
    370                 375         


<210>  118
<211>  408
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(408)
<223>  YlPex12p; GenBank Accession No. CAG81532

<400>  118

Met Asp Tyr Phe Ser Ser Leu Asn Ala Ser Gln Leu Asp Pro Asp Val 
1               5                   10                  15      


Pro Thr Leu Phe Glu Leu Leu Ser Ala Lys Gln Leu Glu Gly Leu Ile 
            20                  25                  30          


Ala Pro Ser Val Arg Tyr Ile Leu Ala Phe Tyr Ala Gln Arg His Pro 
        35                  40                  45              


Arg Tyr Leu Leu Arg Ile Val Asn Arg Tyr Asp Glu Leu Tyr Ala Leu 
    50                  55                  60                  


Phe Met Gly Leu Val Glu Tyr Tyr Asn Leu Lys Thr Trp Asn Ala Ser 
65                  70                  75                  80  


Phe Thr Glu Lys Phe Tyr Gly Leu Lys Arg Thr Gln Ile Leu Thr Asn 
                85                  90                  95      


Pro Ala Leu Arg Thr Arg Gln Ala Val Pro Asp Leu Val Glu Ala Glu 
            100                 105                 110         


Lys Arg Leu Ser Lys Lys Lys Ile Trp Gly Ser Leu Phe Phe Leu Ile 
        115                 120                 125             


Val Val Pro Tyr Val Lys Glu Lys Leu Asp Ala Arg Tyr Glu Arg Leu 
    130                 135                 140                 


Lys Gly Arg Tyr Leu Ala Arg Asp Ile Asn Glu Glu Arg Ile Glu Ile 
145                 150                 155                 160 


Lys Arg Thr Gly Thr Ala Gln Gln Ile Ala Val Phe Glu Phe Asp Tyr 
                165                 170                 175     


Trp Leu Leu Lys Leu Tyr Pro Ile Val Thr Met Gly Cys Thr Thr Ala 
            180                 185                 190         


Thr Leu Ala Phe His Met Leu Phe Leu Phe Ser Val Thr Arg Ala Tyr 
        195                 200                 205             


Ser Ile Asp Asp Phe Leu Leu Asn Ile Gln Phe Ser Arg Met Thr Arg 
    210                 215                 220                 


Tyr Asp Tyr Gln Met Glu Thr Gln Arg Asp Ser Arg Asn Ala Ala Asn 
225                 230                 235                 240 


Val Ala His Thr Met Lys Ser Ile Ser Glu Tyr Pro Val Ala Glu Arg 
                245                 250                 255     


Val Met Leu Leu Leu Thr Thr Lys Ala Gly Ala Asn Ala Met Arg Ser 
            260                 265                 270         


Ala Ala Leu Ser Gly Leu Ser Tyr Val Leu Pro Thr Ser Ile Phe Ala 
        275                 280                 285             


Leu Lys Phe Leu Glu Trp Trp Tyr Ala Ser Asp Phe Ala Arg Gln Leu 
    290                 295                 300                 


Asn Gln Lys Arg Arg Gly Asp Leu Glu Asp Asn Leu Pro Val Pro Asp 
305                 310                 315                 320 


Lys Val Lys Gly Ala Asp Lys Leu Ala Glu Ser Val Ala Lys Trp Lys 
                325                 330                 335     


Glu Asp Thr Ser Lys Cys Pro Leu Cys Ser Lys Glu Leu Val Asn Pro 
            340                 345                 350         


Thr Val Ile Glu Ser Gly Tyr Val Phe Cys Tyr Thr Cys Ile Tyr Arg 
        355                 360                 365             


His Leu Glu Asp Gly Asp Glu Glu Thr Gly Gly Arg Cys Pro Val Thr 
    370                 375                 380                 


Gly Gln Lys Leu Leu Gly Cys Arg Trp Gln Asp Asp Val Trp Gln Val 
385                 390                 395                 400 


Thr Gly Leu Arg Arg Leu Met Val 
                405             


<210>  119
<211>  412
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(412)
<223>  YlPex13p; GenBank Accession No. CAG81789

<400>  119

Met Ser Val Pro Arg Pro Lys Pro Trp Glu Gly Ala Ser Gly Ser Ser 
1               5                   10                  15      


Ala Ala Thr Ala Thr Pro Ala Ala Thr Ala Thr Pro Ala Ser Thr Asp 
            20                  25                  30          


Ala Val Ser Ser Ser Ala Gly Ser Ala Thr Gly Ala Pro Glu Leu Pro 
        35                  40                  45              


Ser Arg Pro Ser Ala Met Gly Ser Thr Ser Asn Ala Leu Ser Ser Pro 
    50                  55                  60                  


Met Gly Ser Ser Met Asn Ser Gly Tyr Gly Gly Met Asn Ser Gly Tyr 
65                  70                  75                  80  


Gly Gly Met Gly Ser Ser Tyr Gly Ser Gly Tyr Gly Ser Ser Tyr Gly 
                85                  90                  95      


Met Gly Ser Ser Tyr Gly Ser Gly Tyr Gly Ser Gly Leu Gly Gly Tyr 
            100                 105                 110         


Gly Ser Tyr Gly Gly Met Gly Gly Met Gly Gly Met Tyr Gly Ser Arg 
        115                 120                 125             


Tyr Gly Gly Tyr Gly Ser Tyr Gly Gly Met Gly Gly Tyr Gly Gly Tyr 
    130                 135                 140                 


Gly Gly Met Gly Gly Gly Pro Met Gly Gln Asn Gly Leu Ala Gly Gly 
145                 150                 155                 160 


Thr Gln Ala Thr Phe Gln Leu Ile Glu Ser Ile Val Gly Ala Val Gly 
                165                 170                 175     


Gly Phe Ala Gln Met Leu Glu Ser Thr Tyr Met Ala Thr Gln Ser Ser 
            180                 185                 190         


Phe Phe Ala Met Val Ser Val Ala Glu Gln Phe Gly Asn Leu Lys Asn 
        195                 200                 205             


Thr Leu Gly Ser Leu Leu Gly Ile Tyr Ala Ile Met Arg Trp Ala Arg 
    210                 215                 220                 


Arg Leu Val Ala Lys Leu Ser Gly Gln Pro Val Thr Gly Ala Asn Gly 
225                 230                 235                 240 


Ile Thr Pro Ala Gly Phe Ala Lys Phe Glu Ala Thr Gly Gly Ala Ala 
                245                 250                 255     


Gly Pro Gly Arg Gly Pro Arg Pro Ser Tyr Lys Pro Leu Leu Phe Phe 
            260                 265                 270         


Leu Thr Ala Val Phe Gly Leu Pro Tyr Leu Leu Gly Arg Leu Ile Lys 
        275                 280                 285             


Ala Leu Ala Ala Lys Gln Glu Gly Met Tyr Asp Glu His Gly Asn Leu 
    290                 295                 300                 


Leu Pro Gly Ala Gln Met Gly Met Gly Gly Pro Gly Met Glu Gly Gly 
305                 310                 315                 320 


Ala Glu Ile Asp Pro Ser Lys Leu Glu Phe Cys Arg Ala Asn Phe Asp 
                325                 330                 335     


Phe Val Pro Glu Asn Pro Gln Leu Glu Leu Glu Leu Arg Lys Gly Asp 
            340                 345                 350         


Leu Val Ala Val Leu Ala Lys Thr Asp Pro Met Gly Asn Pro Ser Gln 
        355                 360                 365             


Trp Trp Arg Val Arg Thr Arg Asp Gly Arg Ser Gly Tyr Val Pro Ala 
    370                 375                 380                 


Asn Tyr Leu Glu Val Ile Pro Arg Pro Ala Val Glu Ala Pro Lys Lys 
385                 390                 395                 400 


Val Glu Glu Ile Gly Ala Ser Ala Val Pro Val Asn 
                405                 410         


<210>  120
<211>  380
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(380)
<223>  YlPex14p; GenBank Accession No. CAG79323

<400>  120

Met Ile Pro Ser Cys Leu Ser Thr Gln His Met Ala Pro Arg Glu Asp 
1               5                   10                  15      


Leu Val Gln Ser Ala Val Ala Phe Leu Asn Asp Pro Gln Ala Ala Thr 
            20                  25                  30          


Ala Pro Leu Ala Lys Arg Ile Glu Phe Leu Glu Ser Lys Asp Met Thr 
        35                  40                  45              


Pro Glu Glu Ile Glu Glu Ala Leu Lys Arg Ala Gly Ser Gly Ser Ala 
    50                  55                  60                  


Gln Ser His Pro Gly Ser Val Val Ser His Gly Gly Ala Ala Pro Thr 
65                  70                  75                  80  


Val Pro Ala Ser Tyr Ala Phe Gln Ser Ala Pro Pro Leu Pro Glu Arg 
                85                  90                  95      


Asp Trp Lys Asp Val Phe Ile Met Ala Thr Val Thr Val Gly Val Gly 
            100                 105                 110         


Phe Gly Leu Tyr Thr Val Ala Lys Arg Tyr Leu Met Pro Leu Ile Leu 
        115                 120                 125             


Pro Pro Thr Pro Pro Ser Leu Glu Ala Asp Lys Glu Ala Leu Glu Ala 
    130                 135                 140                 


Glu Phe Ala Arg Val Gln Gly Leu Leu Asp Gln Val Gln Gln Asp Thr 
145                 150                 155                 160 


Glu Glu Val Lys Asn Ser Gln Val Glu Val Ala Lys Arg Val Thr Asp 
                165                 170                 175     


Ala Leu Lys Gly Val Glu Glu Thr Ile Asp Gln Leu Lys Ser Gln Thr 
            180                 185                 190         


Lys Lys Arg Asp Asp Glu Met Lys Leu Val Thr Ala Glu Val Glu Arg 
        195                 200                 205             


Ile Arg Asp Arg Leu Pro Lys Asn Ile Asp Lys Leu Lys Asp Ser Gln 
    210                 215                 220                 


Glu Gln Gly Leu Ala Asp Ile Gln Ser Glu Leu Lys Ser Leu Lys Gln 
225                 230                 235                 240 


Leu Leu Ser Thr Arg Thr Ala Ala Ser Ser Gly Pro Lys Leu Pro Pro 
                245                 250                 255     


Ile Pro Pro Pro Ser Ser Tyr Leu Thr Arg Lys Ala Ser Pro Ala Val 
            260                 265                 270         


Pro Ala Ala Ala Pro Ala Pro Val Thr Pro Gly Ser Pro Val His Asn 
        275                 280                 285             


Val Ser Ser Ser Ser Thr Val Pro Ala Asp Arg Asp Asp Phe Ile Pro 
    290                 295                 300                 


Thr Pro Ala Gly Ala Val Pro Met Ile Pro Gln Pro Ala Ser Met Ser 
305                 310                 315                 320 


Ser Ser Ser Thr Ser Thr Val Pro Asn Ser Ala Ile Ser Ser Ala Pro 
                325                 330                 335     


Ser Pro Ile Gln Glu Pro Glu Pro Phe Val Pro Glu Pro Gly Asn Ser 
            340                 345                 350         


Ala Val Lys Lys Pro Ala Pro Lys Ala Ser Ile Pro Ala Trp Gln Leu 
        355                 360                 365             


Ala Ala Leu Glu Lys Glu Lys Glu Lys Glu Lys Glu 
    370                 375                 380 


<210>  121
<211>  391
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(391)
<223>  YlPex16p; GenBank Accession No. CAG79622

<400>  121

Met Thr Asp Lys Leu Val Lys Val Met Gln Lys Lys Lys Ser Ala Pro 
1               5                   10                  15      


Gln Thr Trp Leu Asp Ser Tyr Asp Lys Phe Leu Val Arg Asn Ala Ala 
            20                  25                  30          


Ser Ile Gly Ser Ile Glu Ser Thr Leu Arg Thr Val Ser Tyr Val Leu 
        35                  40                  45              


Pro Gly Arg Phe Asn Asp Val Glu Ile Ala Thr Glu Thr Leu Tyr Ala 
    50                  55                  60                  


Val Leu Asn Val Leu Gly Leu Tyr His Asp Thr Ile Ile Ala Arg Ala 
65                  70                  75                  80  


Val Ala Ala Ser Pro Asn Ala Ala Ala Val Tyr Arg Pro Ser Pro His 
                85                  90                  95      


Asn Arg Tyr Thr Asp Trp Phe Ile Lys Asn Arg Lys Gly Tyr Lys Tyr 
            100                 105                 110         


Ala Ser Arg Ala Val Thr Phe Val Lys Phe Gly Glu Leu Val Ala Glu 
        115                 120                 125             


Met Val Ala Lys Lys Asn Gly Gly Glu Met Ala Arg Trp Lys Cys Ile 
    130                 135                 140                 


Ile Gly Ile Glu Gly Ile Lys Ala Gly Leu Arg Ile Tyr Met Leu Gly 
145                 150                 155                 160 


Ser Thr Leu Tyr Gln Pro Leu Cys Thr Thr Pro Tyr Pro Asp Arg Glu 
                165                 170                 175     


Val Thr Gly Glu Leu Leu Glu Thr Ile Cys Arg Asp Glu Gly Glu Leu 
            180                 185                 190         


Asp Ile Glu Lys Gly Leu Met Asp Pro Gln Trp Lys Met Pro Arg Thr 
        195                 200                 205             


Gly Arg Thr Ile Pro Glu Ile Ala Pro Thr Asn Val Glu Gly Tyr Leu 
    210                 215                 220                 


Leu Thr Lys Val Leu Arg Ser Glu Asp Val Asp Arg Pro Tyr Asn Leu 
225                 230                 235                 240 


Leu Ser Arg Leu Asp Asn Trp Gly Val Val Ala Glu Leu Leu Ser Ile 
                245                 250                 255     


Leu Arg Pro Leu Ile Tyr Ala Cys Leu Leu Phe Arg Gln His Val Asn 
            260                 265                 270         


Lys Thr Val Pro Ala Ser Thr Lys Ser Lys Phe Pro Phe Leu Asn Ser 
        275                 280                 285             


Pro Trp Ala Pro Trp Ile Ile Gly Leu Val Ile Glu Ala Leu Ser Arg 
    290                 295                 300                 


Lys Met Met Gly Ser Trp Leu Leu Arg Gln Arg Gln Ser Gly Lys Thr 
305                 310                 315                 320 


Pro Thr Ala Leu Asp Gln Met Glu Val Lys Gly Arg Thr Asn Leu Leu 
                325                 330                 335     


Gly Trp Trp Leu Phe Arg Gly Glu Phe Tyr Gln Ala Tyr Thr Arg Pro 
            340                 345                 350         


Leu Leu Tyr Ser Ile Val Ala Arg Leu Glu Lys Ile Pro Gly Leu Gly 
        355                 360                 365             


Leu Phe Gly Ala Leu Ile Ser Asp Tyr Leu Tyr Leu Phe Asp Arg Tyr 
    370                 375                 380                 


Tyr Phe Thr Ala Ser Thr Leu 
385                 390     


<210>  122
<211>  225
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(225)
<223>  YlPex17p; GenBank Accession No. CAG84025

<400>  122

Met Ser Ala Phe Pro Glu Pro Ser Ser Phe Glu Ile Glu Phe Ala Lys 
1               5                   10                  15      


Gln Met Asn Arg Pro Arg Thr Val Gln Phe Lys Gln Leu Val Ala Val 
            20                  25                  30          


Leu Tyr Ile Phe Gly Gly Thr Ser Ala Leu Ile Tyr Ile Ile Ser Lys 
        35                  40                  45              


Thr Ile Leu Asn Pro Leu Phe Glu Glu Leu Thr Phe Ala Arg Ser Glu 
    50                  55                  60                  


Tyr Ala Ile His Ala Arg Arg Leu Met Glu Gln Leu Asn Ala Lys Leu 
65                  70                  75                  80  


Ser Ser Met Ala Ser Tyr Ile Pro Pro Val Arg Ala Leu Gln Gly Gln 
                85                  90                  95      


Arg Phe Val Asp Ala Gln Thr Gln Thr Glu Asp Glu Glu Gly Glu Asp 
            100                 105                 110         


Ile Pro Asn Pro Ser Leu Gly Lys Ser Ser His Val Ser Phe Gly Glu 
        115                 120                 125             


Ser Pro Met Gln Leu Lys Leu Ala Glu Lys Glu Lys Gln Gln Lys Leu 
    130                 135                 140                 


Ile Asp Asp Ser Val Asp Asn Leu Glu Arg Leu Ala Asp Ser Leu Lys 
145                 150                 155                 160 


His Ala Gly Glu Val Ser Asp Leu Ser Ala Leu Ser Gly Phe Lys Tyr 
                165                 170                 175     


Gln Val Glu Glu Leu Thr Asn Tyr Ser Asp Gln Leu Ala Met Ser Gly 
            180                 185                 190         


Tyr Ser Met Met Lys Ser Gly Leu Pro Gly His Glu Thr Ala Met Ser 
        195                 200                 205             


Glu Thr Lys Lys Glu Ile Arg Ser Leu Lys Gly Ser Val Leu Ser Val 
    210                 215                 220                 


Arg 
225 


<210>  123
<211>  324
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(324)
<223>  YlPex19p; GenBank Accession No. AAK84827

<400>  123

Met Ser His Glu Glu Asp Leu Asp Asp Leu Asp Asp Phe Leu Asp Glu 
1               5                   10                  15      


Phe Asp Glu Gln Val Leu Ser Lys Pro Pro Gly Ala Gln Lys Asp Ala 
            20                  25                  30          


Thr Pro Thr Thr Ser Thr Ala Pro Thr Thr Ala Glu Ala Lys Pro Asp 
        35                  40                  45              


Ala Thr Lys Lys Ser Thr Glu Thr Ser Gly Thr Asp Ser Lys Thr Glu 
    50                  55                  60                  


Gly Ala Asp Thr Ala Asp Lys Asn Ala Ala Thr Asp Ser Ala Glu Ala 
65                  70                  75                  80  


Gly Ala Glu Lys Val Ser Leu Pro Asn Leu Glu Asp Gln Leu Ala Gly 
                85                  90                  95      


Leu Lys Met Asp Asp Phe Leu Lys Asp Ile Glu Ala Asp Pro Glu Ser 
            100                 105                 110         


Lys Ala Gln Phe Glu Ser Leu Leu Lys Glu Ile Asn Asn Val Thr Ser 
        115                 120                 125             


Ala Thr Ala Ser Glu Lys Ala Gln Gln Pro Lys Ser Phe Lys Glu Thr 
    130                 135                 140                 


Ile Ser Ala Thr Ala Asp Arg Leu Asn Gln Ser Asn Gln Glu Met Gly 
145                 150                 155                 160 


Asp Met Pro Leu Gly Asp Asp Met Leu Ala Gly Leu Met Glu Gln Leu 
                165                 170                 175     


Ser Gly Ala Gly Gly Phe Gly Glu Gly Gly Glu Gly Asp Phe Gly Asp 
            180                 185                 190         


Met Leu Gly Gly Ile Met Arg Gln Leu Ala Ser Lys Glu Val Leu Tyr 
        195                 200                 205             


Gln Pro Leu Lys Glu Met His Asp Asn Tyr Pro Lys Trp Trp Asp Glu 
    210                 215                 220                 


His Gly Ser Lys Val Thr Glu Glu Lys Glu Arg Asp Arg Leu Lys Leu 
225                 230                 235                 240 


Gln Gln Asp Ile Val Gly Lys Ile Cys Ala Lys Phe Glu Asp Pro Ser 
                245                 250                 255     


Tyr Ser Asp Asp Ser Glu Ala Asp Arg Ala Val Ile Thr Gln Leu Met 
            260                 265                 270         


Asp Glu Met Gln Glu Thr Gly Ala Pro Pro Asp Glu Ile Met Ser Asn 
        275                 280                 285             


Val Ala Asp Gly Ser Ile Pro Gly Gly Leu Asp Gly Leu Gly Leu Gly 
    290                 295                 300                 


Gly Leu Gly Gly Gly Lys Met Pro Glu Met Pro Glu Asn Met Pro Glu 
305                 310                 315                 320 


Cys Asn Gln Gln 
                


<210>  124
<211>  417
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(417)
<223>  YlPex20p; GenBank Accession No. CAG79226

<400>  124

Met Ala Ser Cys Gly Pro Ser Asn Ala Leu Gln Asn Leu Ser Lys His 
1               5                   10                  15      


Ala Ser Ala Asp Arg Ser Leu Gln His Asp Arg Met Ala Pro Gly Gly 
            20                  25                  30          


Ala Pro Gly Ala Gln Arg Gln Gln Phe Arg Ser Gln Thr Gln Gly Gly 
        35                  40                  45              


Gln Leu Asn Asn Glu Phe Gln Gln Phe Ala Gln Ala Gly Pro Ala His 
    50                  55                  60                  


Asn Ser Phe Glu Gln Ser Gln Met Gly Pro His Phe Gly Gln Gln His 
65                  70                  75                  80  


Phe Gly Gln Pro His Gln Pro Gln Met Gly Gln His Ala Pro Met Ala 
                85                  90                  95      


His Gly Gln Gln Ser Asp Trp Ala Gln Ser Phe Ser Gln Leu Asn Leu 
            100                 105                 110         


Gly Pro Gln Thr Gly Pro Gln His Thr Gln Gln Ser Asn Trp Gly Gln 
        115                 120                 125             


Asp Phe Met Arg Gln Ser Pro Gln Ser His Gln Val Gln Pro Gln Met 
    130                 135                 140                 


Ala Asn Gly Val Met Gly Ser Met Ser Gly Met Ser Ser Phe Gly Pro 
145                 150                 155                 160 


Met Tyr Ser Asn Ser Gln Leu Met Asn Ser Thr Tyr Gly Leu Gln Thr 
                165                 170                 175     


Glu His Gln Gln Thr His Lys Thr Glu Thr Lys Ser Ser Gln Asp Ala 
            180                 185                 190         


Ala Phe Glu Ala Ala Phe Gly Ala Val Glu Glu Ser Ile Thr Lys Thr 
        195                 200                 205             


Ser Asp Lys Gly Lys Glu Val Glu Lys Asp Pro Met Glu Gln Thr Tyr 
    210                 215                 220                 


Arg Tyr Asp Gln Ala Asp Ala Leu Asn Arg Gln Ala Glu His Ile Ser 
225                 230                 235                 240 


Asp Asn Ile Ser Arg Glu Glu Val Asp Ile Lys Thr Asp Glu Asn Gly 
                245                 250                 255     


Glu Phe Ala Ser Ile Ala Arg Gln Ile Ala Ser Ser Leu Glu Glu Ala 
            260                 265                 270         


Asp Lys Ser Lys Phe Glu Lys Ser Thr Phe Met Asn Leu Met Arg Arg 
        275                 280                 285             


Ile Gly Asn His Glu Val Thr Leu Asp Gly Asp Lys Leu Val Asn Lys 
    290                 295                 300                 


Glu Gly Glu Asp Ile Arg Glu Glu Val Arg Asp Glu Leu Leu Arg Glu 
305                 310                 315                 320 


Gly Ala Ser Gln Glu Asn Gly Phe Gln Ser Glu Ala Gln Gln Thr Ala 
                325                 330                 335     


Pro Leu Pro Val His His Glu Ala Pro Pro Pro Glu Gln Ile His Pro 
            340                 345                 350         


His Thr Glu Thr Gly Asp Lys Gln Leu Glu Asp Pro Met Val Tyr Ile 
        355                 360                 365             


Glu Gln Glu Ala Ala Arg Arg Ala Ala Glu Ser Gly Arg Thr Val Glu 
    370                 375                 380                 


Glu Glu Lys Leu Asn Phe Tyr Ser Pro Phe Glu Tyr Ala Gln Lys Leu 
385                 390                 395                 400 


Gly Pro Gln Gly Val Ala Lys Gln Ser Asn Trp Glu Glu Asp Tyr Asp 
                405                 410                 415     


Phe 
    


<210>  125
<211>  195
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(195)
<223>  YlPex22p; GenBank Accession No. CAG77876

<400>  125

Val Pro Arg Cys Thr Ser His Pro Cys Asn Leu Thr Leu His Leu Pro 
1               5                   10                  15      


Val Thr Thr Met Ala Pro Arg Lys Thr Arg Leu Pro Ala Val Ile Gly 
            20                  25                  30          


Ala Ala Ala Ala Ala Ala Ala Val Ala Tyr Leu Val Tyr Ser Phe Val 
        35                  40                  45              


Ala Lys Ser Asn Ser Asp Gln Asp Thr Phe Asp Ser Ser Val Gln Ser 
    50                  55                  60                  


Ser Ser Lys Ser Ser Thr Lys Ser Pro Lys Ser Thr Ala Thr Asn Ser 
65                  70                  75                  80  


Lys Ile Thr Val Val Val Ser Gln Glu Leu Val Gln Ser Gln Leu Val 
                85                  90                  95      


Asp Phe Lys His Leu Met Ser Val His Pro Asn Leu Val Val Ile Val 
            100                 105                 110         


Pro Pro Met Val Ala Asn Lys Phe His Arg Ala Leu Lys Ser Ser Val 
        115                 120                 125             


Gly His Asp His Gly Val Lys Val Ile Arg Cys Asp Thr Asp Val Gly 
    130                 135                 140                 


Val Ile His Val Ile Lys His Ile Arg Pro Asp Leu Ala Leu Ile Ala 
145                 150                 155                 160 


Asp Gly Val Gly Asp Asn Ile Gln Gly Glu Ile Lys Arg Phe Val Gly 
                165                 170                 175     


Ser Ser Glu Ala Leu Ser Gly Asp Val Asn Leu Ala Ala Glu Arg Leu 
            180                 185                 190         


Thr Gly Leu 
        195 


<210>  126
<211>  386
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(386)
<223>  YlPex26p; GenBank Accession No. NC_006072, antisense translation 
       of nucleotides 117230-118387

<400>  126

Met Pro Pro Ala Met Pro Gln Met Thr Thr Ser Thr Leu Leu Thr Asp 
1               5                   10                  15      


Ser Val Thr Ser Ala Val Asn Gln Ala Ala Thr Pro Lys Val Asp Gln 
            20                  25                  30          


Met Tyr Gln Thr Phe Gly Glu Ser Ala Arg Glu Phe Val Asn Lys Asn 
        35                  40                  45              


Phe Tyr Asn Ser Tyr Glu Leu Ile Arg Pro Phe Phe Asp Glu Ile Thr 
    50                  55                  60                  


Ala Lys Gly Ala Gln Gln Asn Gly Ser Thr Val Leu Asp Ala Glu Asn 
65                  70                  75                  80  


Pro His Asn Ile Pro Leu Ser Leu Trp Ile Lys Val Trp Ser Leu Tyr 
                85                  90                  95      


Leu Ala Ile Leu Asp Ala Ser Cys Lys Gln Ala Gly Glu Ala Leu Leu 
            100                 105                 110         


Asn Ser Thr Gly Asp Leu Ser Gly Ser Asp Ser Gly Glu Trp Asn Gln 
        115                 120                 125             


Thr Arg Lys Leu Leu Ala Arg Lys Leu Thr Ser Gly Ser Val Trp Asp 
    130                 135                 140                 


Glu Leu Val Thr Ala Ser Gly Gly Thr Gly Asn Ile His Pro Thr Ile 
145                 150                 155                 160 


Leu Ala Leu Leu Ala Ser Leu Ser Ile Arg His Asp Thr Asp Ala Lys 
                165                 170                 175     


Leu Met Ala Asp Asn Leu Glu Lys Phe Ile Val Thr Tyr Asn Asp Asn 
            180                 185                 190         


Gly Ser Asp Asp Val Lys Thr Lys Thr Ala Phe Tyr Lys Val Leu Asp 
        195                 200                 205             


Leu Tyr Leu Leu Arg Val Leu Pro Asp Leu Gly Gln Trp Asp Val Ala 
    210                 215                 220                 


His Ser Phe Val Asn Asn Thr Asn Leu Phe Ser His Glu Gln Lys Lys 
225                 230                 235                 240 


Glu Met Thr His Lys Leu Asp Gln Ser Gln Lys His Ala Glu Gln Glu 
                245                 250                 255     


His Lys Arg Leu Leu Glu Glu Ala Gln Glu Lys Glu Lys Ser Asp Ala 
            260                 265                 270         


Lys Glu Lys Glu Arg Glu Glu Arg Val Ser Arg Asp Thr Gln Ser Arg 
        275                 280                 285             


Glu Ile Lys Ser Pro Ile Val Asp Ser Ser Thr Ser Ser Arg Asp Val 
    290                 295                 300                 


Thr Arg Asp Thr Thr Arg Glu Leu Ser Lys Ser Ser Arg Gln Pro Arg 
305                 310                 315                 320 


Thr Leu Ser Gln Ile Ile Ser Thr Ser Leu Lys Ser Gln Phe Asp Gly 
                325                 330                 335     


Asn Ala Ile Phe Arg Thr Leu Ala Leu Ile Val Ile Val Ser Leu Ser 
            340                 345                 350         


Ala Ala Asn Pro Leu Ile Arg Lys Arg Val Val Asp Thr Leu Lys Met 
        355                 360                 365             


Leu Trp Ile Lys Ile Leu Gln Thr Leu Ser Met Gly Phe Lys Val Ser 
    370                 375                 380                 


Tyr Leu 
385     


<210>  127
<211>  8739
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY116

<400>  127
ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc gggtggacgt       60

ctagaggtac ctagcaatta acagatagtt tgccggtgat aattctctta acctcccaca      120

ctcctttgac ataacgattt atgtaacgaa actgaaattt gaccagatat tgtgtccgcg      180

gtggagctcc agcttttgtt ccctttagtg agggtttaaa cgagcttggc gtaatcatgg      240

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc      300

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg      360

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc      420

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact      480

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta      540

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag      600

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc      660

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta      720

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg      780

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc      840

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac      900

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac      960

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg     1020

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga     1080

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt     1140

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag     1200

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct     1260

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg     1320

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat     1380

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc     1440

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg     1500

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct     1560

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca     1620

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg     1680

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg     1740

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc     1800

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag     1860

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg     1920

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag     1980

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat     2040

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg     2100

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca     2160

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca     2220

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat     2280

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag     2340

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc     2400

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt     2460

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc     2520

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta     2580

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc     2640

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg     2700

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt     2760

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat     2820

tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg     2880

aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg     2940

caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg     3000

ccagtgaatt gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg     3060

tcgatggtgt cgataagctt gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa     3120

ggaaacctaa ttctacatcc gagagactgc cgagatccag tctacactga ttaattttcg     3180

ggccaataat ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat     3240

gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc cgcctccaac     3300

tgatgttctc aatatttaag gggtcatctc gcattgttta ataataaaca gactccatct     3360

accgcctcca aatgatgttc tcaaaatata ttgtatgaac ttatttttat tacttagtat     3420

tattagacaa cttacttgct ttatgaaaaa cacttcctat ttaggaaaca atttataatg     3480

gcagttcgtt catttaacaa tttatgtaga ataaatgtta taaatgcgta tgggaaatct     3540

taaatatgga tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa     3600

aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa gaacagctat     3660

tcacacgtta ctattgagat tattattgga cgagaatcac acactcaact gtctttctct     3720

cttctagaaa tacaggtaca agtatgtact attctcattg ttcatacttc tagtcatttc     3780

atcccacata ttccttggat ttctctccaa tgaatgacat tctatcttgc aaattcaaca     3840

attataataa gatataccaa agtagcggta tagtggcaat caaaaagctt ctctggtgtg     3900

cttctcgtat ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat     3960

ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt taattttttg     4020

cttaaattca atcccccctc gttcagtgtc aactgtaatg gtaggaaatt accatacttt     4080

tgaagaagca aaaaaaatga aagaaaaaaa aaatcgtatt tccaggttag acgttccgca     4140

gaatctagaa tgcggtatgc ggtacattgt tcttcgaacg taaaagttgc gctccctgag     4200

atattgtaca tttttgcttt tacaagtaca agtacatcgt acaactatgt actactgttg     4260

atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta     4320

ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc aattaatcat     4380

agacttatga atctgcacgg tgtgcgctgc gagttacttt tagcttatgc atgctacttg     4440

ggtgtaatat tgggatctgt tcggaaatca acggatgctc aaccgatttc gacagtaatt     4500

aattaatttg aatcgaatcg gagcctaaaa tgaacccgag tatatctcat aaaattctcg     4560

gtgagaggtc tgtgactgtc agtacaaggt gccttcatta tgccctcaac cttaccatac     4620

ctcactgaat gtagtgtacc tctaaaaatg aaatacagtg ccaaaagcca aggcactgag     4680

ctcgtctaac ggacttgata tacaaccaat taaaacaaat gaaaagaaat acagttcttt     4740

gtatcatttg taacaattac cctgtacaaa ctaaggtatt gaaatcccac aatattccca     4800

aagtccaccc ctttccaaat tgtcatgcct acaactcata taccaagcac taacctacca     4860

aacaccacta aaaccccaca aaatatatct taccgaatat acagtaacaa gctaccacca     4920

cactcgttgg gtgcagtcgc cagcttaaag atatctatcc acatcagcca caactccctt     4980

cctttaataa accgactaca cccttggcta ttgaggttat gagtgaatat actgtagaca     5040

agacactttc aagaagactg tttccaaaac gtaccactgt cctccactac aaacacaccc     5100

aatctgcttc ttctagtcaa ggttgctaca ccggtaaatt ataaatcatc atttcattag     5160

cagggcaggg ccctttttat agagtcttat acactagcgg accctgccgg tagaccaacc     5220

cgcaggcgcg tcagtttgct ccttccatca atgcgtcgta gaaacgactt actccttctt     5280

gagcagctcc ttgaccttgt tggcaacaag tctccgacct cggaggtgga ggaagagcct     5340

ccgatatcgg cggtagtgat accagcctcg acggactcct tgacggcagc ctcaacagcg     5400

tcaccggcgg gcttcatgtt aagagagaac ttgagcatca tggcggcaga cagaatggtg     5460

gcaatggggt tgaccttctg cttgccgaga tcgggggcag atccgtgaca gggctcgtac     5520

agaccgaacg cctcgttggt gtcgggcaga gaagccagag aggcggaggg cagcagaccc     5580

agagaaccgg ggatgacgga ggcctcgtcg gagatgatat cgccaaacat gttggtggtg     5640

atgatgatac cattcatctt ggagggctgc ttgatgagga tcatggcggc cgagtcgatc     5700

agctggtggt tgagctcgag ctgggggaat tcgtccttga ggactcgagt gacagtcttt     5760

cgccaaagtc gagaggaggc cagcacgttg gccttgtcaa gagaccacac gggaagaggg     5820

gggttgtgct gaagggccag gaaggcggcc attcgggcaa ttcgctcaac ctcaggaacg     5880

gagtaggtct cggtgtcgga agcgacgcca gatccgtcat cctcctttcg ctctccaaag     5940

tagatacctc cgacgagctc tcggacaatg atgaagtcgg tgccctcaac gtttcggatg     6000

ggggagagat cggcgagctt gggcgacagc agctggcagg gtcgcaggtt ggcgtacagg     6060

ttcaggtcct ttcgcagctt gaggagaccc tgctcgggtc gcacgtcggt tcgtccgtcg     6120

ggagtggtcc atacggtgtt ggcagcgcct ccgacagcac cgagcataat agagtcagcc     6180

tttcggcaga tgtcgagagt agcgtcggtg atgggctcgc cctccttctc aatggcagct     6240

cctccaatga gtcggtcctc aaacacaaac tcggtgccgg aggcctcagc aacagacttg     6300

agcaccttga cggcctcggc aatcacctcg gggccacaga agtcgccgcc gagaagaaca     6360

atcttcttgg agtcagtctt ggtcttctta gtttcgggtt ccattgtgga tgtgtgtggt     6420

tgtatgtgtg atgtggtgtg tggagtgaaa atctgtggct ggcaaacgct cttgtatata     6480

tacgcacttt tgcccgtgct atgtggaaga ctaaacctcc gaagattgtg actcaggtag     6540

tgcggtatcg gctagggacc caaaccttgt cgatgccgat agcgctatcg aacgtacccc     6600

agccggccgg gagtatgtcg gaggggacat acgagatcgt caagggtttg tggccaactg     6660

gtatttaaat gtagctaacg gtagcaggcg aactactggt acatacctcc cccggaatat     6720

gtacaggcat aatgcgtatc tgtgggacat gtggtcgttg cgccattatg taagcagcgt     6780

gtactcctct gactgtccat atggtttgct ccatctcacc ctcatcgttt tcattgttca     6840

caggcggcca caaaaaaact gtcttctctc cttctctctt cgccttagtc tactcggacc     6900

agttttagtt tagcttggcg ccactggata aatgagacct caggccttgt gatgaggagg     6960

tcacttatga agcatgttag gaggtgcttg tatggataga gaagcaccca aaataataag     7020

aataataata aaacaggggg cgttgtcatt tcatatcgtg ttttcaccat caatacacct     7080

ccaaacaatg cccttcatgt ggccagcccc aatattgtcc tgtagttcaa ctctatgcag     7140

ctcgtatctt attgagcaag taaaactctg tcagccgata ttgcccgacc cgcgacaagg     7200

gtcaacaagg tggtgtaagg ccttcgcaga agtcaaaact gtgccaaaca aacatctaga     7260

gtctctttgg tgtttctcgc atatatttwa tcggctgtct tacgtatttg cgcctcggta     7320

ccggactaat ttcggatcat ccccaatacg ctttttcttc gcagctgtca acagtgtcca     7380

tgatctatcc acctaaatgg gtcatatgag gcgtataatt tcgtggtgct gataataatt     7440

cccatatatt tgacacaaaa cttccccccc tagacataca tctcacaatc tcacttcttg     7500

tgcttctgtc acacatctcc tccagctgac ttcaactcac acctctgccc cagttggtct     7560

acagcggtat aaggtttctc cgcatagagg tgcaccactc ctcccgatac ttgtttgtgt     7620

gacttgtggg tcacgacata tatatctaca cacattgcgc caccctttgg ttcttccagc     7680

acaacaaaaa cacgacacgc taaccatggc caatttactg accgtacacc aaaatttgcc     7740

tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag     7800

ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg     7860

ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg     7920

cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt     7980

gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc     8040

tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa     8100

acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag     8160

cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt     8220

acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag     8280

aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc     8340

acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga     8400

tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc     8460

caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat     8520

ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg     8580

tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc     8640

tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac     8700

aggggcaatg gtgcgcctgc tggaagatgg cgattaagc                            8739


<210>  128
<211>  13975
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKSL-5S5A5

<400>  128
cgatggttaa tgctgctgtg tgctgtgtgt gtgtgttgtt tggcgctcat tgttgcgtta       60

tgcagcgtac accacaatat tggaagctta ttagcctttc tattttttcg tttgcaaggc      120

ttaacaacat tgctgtggag agggatgggg atatggaggc cgctggaggg agtcggagag      180

gcgttttgga gcggcttggc ctggcgccca gctcgcgaaa cgcacctagg accctttggc      240

acgccgaaat gtgccacttt tcagtctagt aacgccttac ctacgtcatt ccatgcgtgc      300

atgtttgcgc cttttttccc ttgcccttga tcgccacaca gtacagtgca ctgtacagtg      360

gaggttttgg gggggtctta gatgggagct aaaagcggcc tagcggtaca ctagtgggat      420

tgtatggagt ggcatggagc ctaggtggag cctgacagga cgcacgaccg gctagcccgt      480

gacagacgat gggtggctcc tgttgtccac cgcgtacaaa tgtttgggcc aaagtcttgt      540

cagccttgct tgcgaaccta attcccaatt ttgtcacttc gcacccccat tgatcgagcc      600

ctaacccctg cccatcaggc aatccaatta agctcgcatt gtctgccttg tttagtttgg      660

ctcctgcccg tttcggcgtc cacttgcaca aacacaaaca agcattatat ataaggctcg      720

tctctccctc ccaaccacac tcactttttt gcccgtcttc ccttgctaac acaaaagtca      780

agaacacaaa caaccacccc aaccccctta cacacaagac atatctacag caatggccat      840

ggctctcagt cttaccacag aacagctgtt agaacgccct gatttggttg cgattgatgg      900

catcctctac gaccttgaag ggcttgccaa agttcatcca ggatccgatt tgattctcgc      960

ttctggtgcc tctgatgcct cccctctctt ttattcaatg catccatacg tcaaaccgga     1020

gaactccaaa ttgcttcaac agttcgtccg agggaagcat gaccgcacct cgaaggacat     1080

tgtctacacg tatgattctc ccttcgcaca agacgttaag cggacaatgc gcgaggtgat     1140

gaaagggagg aactggtacg caacccctgg cttctggctg cgcaccgttg ggatcatcgc     1200

cgtgacggcc ttttgcgagt ggcactgggc taccacgggg atggtgctgt ggggcctgtt     1260

gactggattc atgcacatgc agatcggctt atccatccag catgatgcgt cccacggggc     1320

catcagcaag aagccttggg tcaacgccct cttcgcctac ggcattgacg tcatcggatc     1380

gtcccggtgg atttggctgc agtcgcacat catgcggcac cacacctaca ccaaccagca     1440

cggcctcgac ctggatgcgg agtcggcaga gccgttcctg gtgttccaca actaccccgc     1500

cgcaaacacc gcccgaaagt ggttccaccg cttccaggct tggtacatgt accttgtgct     1560

gggggcatac ggggtatcgc tggtgtacaa cccgctctac attttccgga tgcagcacaa     1620

tgacaccatc ccagagtctg tcacggccat gcgggaaaat ggctttctgc ggcgctaccg     1680

cacacttgca ttcgtgatgc gagctttctt catcttccgg accgcattct tgccctggta     1740

cctcactggg acctcattgc tgatcaccat tcctctggtg cccaccgcaa ctggtgcctt     1800

cttgacgttc ttcttcattt tgtcccacaa ttttgatggc tccgaacgga tccccgacaa     1860

gaactgcaag gttaagcgat ctgagaagga cgttgaggct gaccaaattg actggtatcg     1920

ggcgcaggtg gagacgtcct ccacatacgg tggccccatc gccatgttct tcactggcgg     1980

tctcaatttc cagatcgagc accacctctt tccccggatg tcgtcttggc actacccctt     2040

cgtccagcag gcggtccggg agtgttgcga acgacatgga gtgcgatatg ttttctaccc     2100

taccatcgtc ggcaacatca tctccaccct gaagtacatg cataaggtgg gtgtcgtcca     2160

ctgcgtgaag gacgcacagg attcctaagc ggccgcattg atgattggaa acacacacat     2220

gggttatatc taggtgagag ttagttggac agttatatat taaatcagct atgccaacgg     2280

taacttcatt catgtcaacg aggaaccagt gactgcaagt aatatagaat ttgaccacct     2340

tgccattctc ttgcactcct ttactatatc tcatttattt cttatataca aatcacttct     2400

tcttcccagc atcgagctcg gaaacctcat gagcaataac atcgtggatc tcgtcaatag     2460

agggcttttt ggactccttg ctgttggcca ccttgtcctt gctgtctggc tcattctgtt     2520

tcaacgcctt ttaattaact ctcctgcagc ttcctcagcg agacactgct cctgtctgga     2580

cccgagctaa ggctctgttc gacaaacacg ttctgcgaat tggcgagtaa tttcattaat     2640

gcaaatagac gtgtatttga aaaggaggag atgatggtac cagtaattta cggtgtttga     2700

atagacaaat tatatatata aaattaacct gatgaatgag tgtatgaatg gattgcttaa     2760

tataccgagg gagagccggc attatcaata tttattgtcc taagaagcta aaatatgctg     2820

ttccgttgat agcgatgtct gtttgagagt caatggcaga acgcggagga gtattgttag     2880

ttggtgatcg gtttactcga catcgagtag ggggcgagac agaagatatc ccgaatccat     2940

tgcattgttt attaggatgt tcacaacaca actccctcta gactctgggg atgtgcgtta     3000

gaagaatgac ctggagcaag agtgttcaga ttgatccgtt caaattttca agattactgt     3060

tggggttgtt tttgaatcca cattagctgg acgactattg catctgagcg ctcggaacgt     3120

ctccctttcc cttcagcttt atacgagtcg attccataag cgcgacctct cgaattgcct     3180

tcggttgtga agccataggc aaaggtgtgg ctatggaatg catgcgacgt cgggcccaat     3240

tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg     3300

gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg     3360

cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc     3420

gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg     3480

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc     3540

tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc     3600

gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta     3660

gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta     3720

atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg     3780

atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa     3840

aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct     3900

ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc     3960

gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac     4020

aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt     4080

tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag     4140

aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg     4200

aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa     4260

tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc     4320

aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag     4380

tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa     4440

ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc     4500

taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg     4560

agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa     4620

caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa     4680

tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg     4740

gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag     4800

cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg     4860

caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt     4920

ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt     4980

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     5040

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     5100

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     5160

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     5220

gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga     5280

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     5340

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     5400

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     5460

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     5520

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     5580

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     5640

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     5700

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     5760

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     5820

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca     5880

aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcgcgcc gaggtttcca     5940

acgagataac atcgtggcag ctgccaccat gaccgatatg cctgaaataa gtgaattgac     6000

catcagaagt tctgcattca aatagaacta tatcatattc ggctcagttt tttcaataat     6060

agtccaatcc ctaagtctcc tatccaaaat ggttcctgac cagccacatc catgatcatg     6120

actgcgtgac aggaacagtc attccgtgga tgaacgactt tacgctcagg tactgtaaat     6180

atctgtaaag ggcagacaac caaccaattg agtaacctgt gagacttgaa acgtaagatg     6240

acttcacaca caaagtcact tgactcaacc gtggctctca attgcacaaa atcactctgc     6300

actaatctat tgcaggagtc aggctatgaa caactagacg acagctactt atgtgttata     6360

tagaggaata ttaaaaaatc taagaataat cataaagtta caaaataatt atcagatttc     6420

gagccacagg tcacccctaa catgtgttat tgcacaccca caatcctcag cttgatgtca     6480

tttaattctt ccagccacca tctctctctc caaccctaat ggcaaacttt attttggtgg     6540

agcgatgact cttactcaac tgcagcatac ttaagcacaa ttgttcccca gcctgatacg     6600

acacaccatc cattgtcaag cttcaccaca tacaacaaca cagcgtacgc aactaacatg     6660

aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaaga     6720

gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt     6780

atgtttagta ttgtacttgg acagtgcttg tatcgtacat tctcaagtgt caaacataaa     6840

tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat     6900

ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttg     6960

cggccgctta ggaatcctga gcgtccttga cacagtgaac cacaccgact ttgtgcatgt     7020

acttgagggt ggaaatgatg ttgcccacaa tggtagggta gaagacgtac cgaactccgt     7080

gtcgttcgca acactctcgg acagcttgct gcacgaaggg atagtgccaa gacgacattc     7140

gaggaaagag gtgatgctcg atctggaagt tgagaccgcc agtaaagaac atggcaatgg     7200

gtccaccgta ggtggaagag gtctccacct gagctctgta ccagtcgatc tgatcggctt     7260

caacgtcctt ctcggagctc ttgaccttgc agttcttgtc ggggattcgc tccgagccat     7320

cgaagttgtg agacaagatg aaaaagaagg tgaggaaggc accggtagca gtgggcacca     7380

gaggaatggt gatgagcagg gaggttccag tgagatacca gggcaagaag gcggttcgaa     7440

agatgaagaa agctcgcata acgaatgcaa gggttcggta ccgtcgcaga aagccgttct     7500

ctcgcatggc tgtgacagac tcgggaatgg tgtcgttgtg ctgcattcgg aagatgtaga     7560

gagggttgta caccagcgaa acgccgtagg ctccaagcac gaggtacatg taccaggcct     7620

ggaatcggtg aaaccacttt cgagcagtgt tggcagcagg gtagttgtgg aacacaagga     7680

atggttctgc ggactcggca tccaggtcga gaccatgctg attggtgtag gtgtgatgtc     7740

gcatgatgtg agactgcagc cagatccatc tggacgatcc aatgacgtcg atgccgtagg     7800

caaagagagc gttgacccag ggctttttgc tgatggcacc atgagaggca tcgtgctgaa     7860

tggacaggcc gatctgcatg tgcatgaatc cagtcaagag accccacagc accattccgg     7920

tagtagccca gtgccactcg caaaaggcgg tgacagcaat gatgccaacg gttcgcagcc     7980

agaatccagg tgtggcatac cagttccgac ctttcatgac ctctcgcata gttcgcttga     8040

cgtcctgtgc aaagggagag tcgtaggtgt agacaatgtc cttggaggtt cggtcgtgct     8100

tgcctcgcac gaactgttga agcagcttcg agttctcggg cttgacgtaa gggtgcatgg     8160

agtagaacag aggagaagca tcggaggcac cagaagcgag gatcaagtcg gatccgggat     8220

ggaccttggc aagaccttcc agatcgtaga gaatgccgtc gatggcaacc aggtcgggtc     8280

gctcgagcag ctgctcggta gtaagggaga gagccatgga gagctgggtt agtttgtgta     8340

gagagtgtgt gttgctagcg actttcggat tgtgtcatta cacaaaacgc gtcgtctcga     8400

cactgatctt gtcgtggata ctcacggctc ggacatcgtc gccgacgatg acaccggact     8460

ttcgcttaag gacgtcagta acaggcattg tgtgatgtgt agtttagatt tcgaatctgt     8520

ggggaaagaa aggaaaaaag agactggcaa ccgattggga gagccactgt ttatatatac     8580

cctagacaag ccccccgctt gtaagatgtt ggtcaatgta aaccagtatt aaggttggca     8640

agtgcaggag aagcaaggtg tgggtaccga gcaatggaaa tgtgcggaag gcaaaaaaat     8700

gaggccacgg cctattgtcg gggctatatc cagggggcga ttgaagtaca ctaacatgac     8760

atgtgtccac agaccctcaa tctggcctga tgagccaaat ccatacgcgc tttcgcagct     8820

ctaaaggcta taacaagtca caccaccctg ctcgacctca gcgccctcac tttttgttaa     8880

gacaaactgt acacgctgtt ccagcgtttt ctgcctgcac ctggtgggac atttggtgca     8940

acctaaagtg ctcggaacct ctgtggtgtc cagatcagcg cagcagttcc gaggtagttt     9000

tgaggccctt agatgatgca atggtgtcag tcgctggatc acgagtctta atggcagtat     9060

tcgttcttat ttgtgccatt gagccccgtt atcctcgtat cttctacccc ccatcccatc     9120

cctttgttgg tgcaacccta cccatttatt gttgggtgca gcccaaccga cgtggagagc     9180

ttggcttggc catataaaaa ggcccccccc tagtggcaat ggcagaaagt cagctgtgag     9240

ttgttgaatt tgtcatctag gcggcctggc cgtcttctcc ggggcaattg gggctgtttt     9300

ttgggacaca aatacgccgc caacccggtc tctcctgaat tctgcagatg ggctgcagga     9360

attccgtcgt cgcctgagtc gactccaact tttcacactg agcgtaaaat gtggagaaga     9420

aatcggcact aaaaagtcag gtagactgga aaatgcgcca tgaaatgaat atctcttgct     9480

acagtaatgc ccagcatcga ggggtattgt gtcaccaaca ctatagtggc agctgaagcg     9540

ctcgtgattg tagtatgagt ctttattggt gatgggaaga gttcactcaa tattctcgtt     9600

actgccaaaa caccacggta atcggccaga caccatggat gtagatcacc aagcctgtga     9660

atgttattcg agctaaaatg cacatggttg gtgaaaggag tagttgctgt cgaattccgt     9720

cgtcgcctga gtcatcattt atttaccagt tggccacaaa cccttgacga tctcgtatgt     9780

cccctccgac atactcccgg ccggctgggg tacgttcgat agcgctatcg gcatcgacaa     9840

ggtttgggtc cctagccgat accgcactac ctgagtcaca atcttcggag gtttagtctt     9900

ccacatagca cgggcaaaag tgcgtatata tacaagagcg tttgccagcc acagattttc     9960

actccacaca ccacatcaca catacaacca cacacatcca caatggaacc cgaaactaag    10020

aagaccaaga ctgactccaa gaagattgtt cttctcggcg gcgacttctg tggccccgag    10080

gtgattgccg aggccgtcaa ggtgctcaag tctgttgctg aggcctccgg caccgagttt    10140

gtgtttgagg accgactcat tggaggagct gccattgaga aggagggcga gcccatcacc    10200

gacgctactc tcgacatctg ccgaaaggct gactctatta tgctcggtgc tgtcggaggc    10260

gctgccaaca ccgtatggac cactcccgac ggacgaaccg acgtgcgacc cgagcagggt    10320

ctcctcaagc tgcgaaagga cctgaacctg tacgccaacc tgcgaccctg ccagctgctg    10380

tcgcccaagc tcgccgatct ctcccccatc cgaaacgttg agggcaccga cttcatcatt    10440

gtccgagagc tcgtcggagg tatctacttt ggagagcgaa aggaggatga cggatctggc    10500

gtcgcttccg acaccgagac ctactccgtt cctgaggttg agcgaattgc ccgaatggcc    10560

gccttcctgg cccttcagca caacccccct cttcccgtgt ggtctcttga caaggccaac    10620

gtgctggcct cctctcgact ttggcgaaag actgtcactc gagtcctcaa ggacgaattc    10680

ccccagctcg agctcaacca ccagctgatc gactcggccg ccatgatcct catcaagcag    10740

ccctccaaga tgaatggtat catcatcacc accaacatgt ttggcgatat catctccgac    10800

gaggcctccg tcatccccgg ttctctgggt ctgctgccct ccgcctctct ggcttctctg    10860

cccgacacca acgaggcgtt cggtctgtac gagccctgtc acggatctgc ccccgatctc    10920

ggcaagcaga aggtcaaccc cattgccacc attctgtctg ccgccatgat gctcaagttc    10980

tctcttaaca tgaagcccgc cggtgacgct gttgaggctg ccgtcaagga gtccgtcgag    11040

gctggtatca ctaccgccga tatcggaggc tcttcctcca cctccgaggt cggagacttg    11100

ttgccaacaa ggtcaaggag ctgctcaaga aggagtaagt cgtttctacg acgcattgat    11160

ggaaggagca aactgacgcg cctgcgggtt ggtctaccgg cagggtccgc tagtgtataa    11220

gactctataa aaagggccct gccctgctaa tgaaatgatg atttataatt taccggtgta    11280

gcaaccttga ctagaagaag cagattgggt gtgtttgtag tggaggacag tggtacgttt    11340

tggaaacagt cttcttgaaa gtgtcttgtc tacagtatat tcactcataa cctcaatagc    11400

caagggtgta gtcggtttat taaaggaagg gagttgtggc tgatgtggat agatatcttt    11460

aagctggcga ctgcacccaa cgagtgtggt ggtagcttgt ttaaacagag tgtgaaagac    11520

tcactatggt ccgggcttat ctcgaccaat agccaaagtc tggagtttct gagagaaaaa    11580

ggcaagatac gtatgtaaca aagcgacgca tggtacaata ataccggagg catgtatcat    11640

agagagttag tggttcgatg atggcactgg tgcctggtat gactttatac ggctgactac    11700

atatttgtcc tcagacatac aattacagtc aagcacttac ccttggacat ctgtaggtac    11760

cccccggcca agacgatctc agcgtgtcgt atgtcggatt ggcgtagctc cctcgctcgt    11820

caattggctc ccatctactt tcttctgctt ggctacaccc agcatgtctg ctatggctcg    11880

ttttcgtgcc ttatctatcc tcccagtatt accaactcta aatgacatga tgtgattggg    11940

tctacacttt catatcagag ataaggagta gcacagttgc ataaaaagcc caactctaat    12000

cagcttcttc ctttcttgta attagtacaa aggtgattag cgaaatctgg aagcttagtt    12060

ggccctaaaa aaatcaaaaa aagcaaaaaa cgaaaaacga aaaaccacag ttttgagaac    12120

agggaggtaa cgaaggatcg tatatatata tatatatata tatacccacg gatcccgaga    12180

ccggcctttg attcttccct acaaccaacc attctcacca ccctaattca caaccatggc    12240

caccatctcc ctgactaccg agcagctcct ggaacacccc gagctcgttg ccatcgacgg    12300

agtcctgtac gatctcttcg gtctggccaa ggtccatgcc ggaggcaacc tcatcgaagc    12360

tgccggtgca tccgacggaa ccgctctgtt ctactccatg catcctggag tcaagccaga    12420

gaactcgaag cttctgcagc aatttgcccg aggcaagcac gaacgaagct ccaaggatcc    12480

cgtgtacacc ttcgactctc cctttgctca ggacgtcaag cagtccgttc gagaggtcat    12540

gaagggtcga aactggtacg ccactcctgg cttctggctg agaaccgcac tcatcatcgc    12600

ttgtactgcc attggcgagt ggtactggat cacaaccgga gcagtgatgt ggggtatctt    12660

tactggatac ttccactcgc agattggctt ggccattcaa cacgatgctt ctcacggagc    12720

catcagcaaa aagccctggg tcaacgcctt tttcgcttat ggcatcgacg ccattggttc    12780

ctctcgttgg atctggctgc agtcccacat tatgcgacat cacacttaca ccaaccagca    12840

tggcctcgac ctggatgctg cctcggcaga gccgttcatc ttgttccact cctatcctgc    12900

taccaacgcc tctcgaaagt ggtaccaccg atttcaggcg tggtacatgt acatcgttct    12960

gggaatgtat ggtgtctcga tggtgtacaa tcccatgtac ctcttcacaa tgcagcacaa    13020

cgacaccatt cccgaggcca cttctctcag accaggcagc tttttcaatc ggcagcgagc    13080

tttcgccgtt tcccttcgac tgctcttcat cttccgaaac gcctttcttc cctggtacat    13140

tgctggtgcc tctcctctgc tcaccattct tctggtgccc acggtcacag gcatcttcct    13200

cacctttgtg ttcgttctgt cccataactt cgagggagcc gaacggaccc cagagaagaa    13260

ctgcaaggcc aaacgagcta aggaaggcaa ggaggtcaga gacgtggaag aggatcgagt    13320

cgactggtac cgagcacagg ccgagactgc tgccacctac ggtggcagcg tgggaatgat    13380

gcttacaggc ggtctcaacc tgcagatcga gcatcacttg tttccccgaa tgtcctcttg    13440

gcactatccc ttcattcaag acaccgttcg ggagtgttgc aagcgacatg gcgtccgtta    13500

cacatactat cctaccattc tcgagaacat catgtccact cttcgataca tgcagaaggt    13560

gggtgttgct cacaccattc aggatgccca ggagttctaa gcggccgcat gtacatacaa    13620

gattatttat agaaatgaat cgcgatcgaa caaagagtac gagtgtacga gtaggggatg    13680

atgataaaag tggaagaagt tccgcatctt tggatttatc aacgtgtagg acgatacttc    13740

ctgtaaaaat gcaatgtctt taccataggt tctgctgtag atgttattaa ctaccattaa    13800

catgtctact tgtacagttg cagaccagtt ggagtataga atggtacact taccaaaaag    13860

tgttgatggt tgtaactacg atatataaaa ctgttgacgg gatccccgct gatatgccta    13920

aggaacaatc aaagaggaag atattaattc agaatgctag tatacagtta gggat         13975


<210>  129
<211>  13066
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZP3-Pa777U

<400>  129
tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa       60

atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt      120

cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca      180

cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata      240

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga      300

gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc      360

ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg      420

cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc      480

ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat      540

cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact      600

tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat      660

tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga      720

tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc      780

ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga      840

tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag      900

cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc      960

gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt     1020

ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct     1080

acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg     1140

cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg     1200

atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca     1260

tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga     1320

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa     1380

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga     1440

aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt     1500

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt     1560

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat     1620

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct     1680

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca     1740

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag     1800

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc     1860

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga     1920

aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca     1980

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag     2040

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg     2100

aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct     2160

ggcgcgccac caatcacaat tctgaaaagc acatcttgat ctcctcattg cggggagtcc     2220

aacggtggtc ttattccccc gaatttcccg ctcaatctcg ttccagaccg acccggacac     2280

agtgcttaac gccgttccga aactctaccg cagatatgct ccaacggact gggctgcata     2340

gatgtgatcc tcggcttgga gaaatggata aaagccggcc aaaaaaaaag cggaaaaaag     2400

cggaaaaaaa gagaaaaaaa atcgcaaaat ttgaaaaata gggggaaaag acgcaaaaac     2460

gcaaggaggg gggagtatat gacactgata agcaagctca caacggttcc tcttattttt     2520

ttcctcatct tctgcctagg ttcccaaaat cccagatgct tctctccagt gccaaaagta     2580

agtaccccac aggttttcgg ccgaaaattc cacgtgcagc aacgtcgtgt ggggtgttaa     2640

aatgtggggg gggggaacca ggacaagagg ctcttgtggg agccgaatga gagcacaaag     2700

cgggcgggtg tgataagggc atttttgccc attttccctt ctcctgtctc tccgacggtg     2760

atggcgttgt gcgtcctcta tttcttttta tttctttttg ttttatttct ctgactaccg     2820

atttggtttg atttcctcaa ccccacacaa ataagctcgg gccgaggaat atatatatac     2880

acggacacag tcgccctgtg gacaacacgt cactacctct acgatacaca ccgtacgttg     2940

tgtggaagct tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg     3000

ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggacaca     3060

atatctggtc aaatttcagt ttcgttacat ttaaattcct tcacttcaag ttcattcttc     3120

atctgcttct gttttacttt gacaggcaaa tgaagacatg gtacgacttg atggaggcca     3180

agaacgccat ttcaccccga gacaccgaag tgcctgaaat cctggctgcc cccattgata     3240

acatcggaaa ctacggtatt ccggaaagtg tatatagaac ctttccccag cttgtgtctg     3300

tggatatgga tggtgtaatc ccctttgagt actcgtcttg gcttctctcc gagcagtatg     3360

aggctctcta atctagcgca tttaatatct caatgtattt atatatttat cttctcatgc     3420

ggccgcttag ttggctttgg tcttggcagc cttggcctcc ttgagggtaa acatcttggc     3480

atccttgtcg accacgccgt acttggcgta cataagacca attcggatga aggtgggaat     3540

gatgggagaa gccgactttc gcaccagttc gggaaaggcc tgagcgaagg cagcagtggc     3600

ctcgttgagc ttgtagtgag gaatgatggg aaacagatgg tggatctgat gtgtaccaat     3660

gttgtgggac aggttgtcga tgagggctcc gtagcttcgg tccacagagg acaagttgcc     3720

cttgacatag gtccactccg aatcggcgta ccagggagtt tcctcgtcgt tgtgatggag     3780

gaaggtagtg acaaccagca tggtggcgaa tccaaagaga ggtgcgaagt aatacagagc     3840

catggtcttg aggccgtaga cgtaggtaag gtaggcgtac agaccagcaa aggccacgag     3900

agagccgagg gaaatgatga cggcagacat tcttcgcagg tagagaggct cccagggatt     3960

gaagtggttg acctttcggg gaggaaatcc agcaacgagg taggcaaacc aagccgaacc     4020

aagggagatg accatgtgtc gggacagggg atgagagtcg gcttctcgct gagggtagaa     4080

gatctcatcc ttgtcgatgt tgccggtgtt cttgtgatgg tgtcgatggc tgatcttcca     4140

cgactcgtag ggagtcagaa tgatggagtg aatgagtgtg ccaacagaga agttgagcag     4200

gtgggatcgc gagaaggcac catgtccaca gtcgtgaccg atggtaaaga atccccagaa     4260

cacgataccc tggagcagaa tgtagccagt gcaaaggacg gcatcgagca gtgcaaactc     4320

ctgcacgata gcaagggctc gagcatagta cagtccgaga gcaagggaac cggcaatgcc     4380

cagagctcgc acggtatagt agagggacca gggaacagag gcttcgaagc agtgggcagg     4440

cagggatcgc ttgatctcgg tgagagtagg gaactcgtag ggagcggcaa cggtagagga     4500

agccatggtt gtgaattagg gtggtgagaa tggttggttg tagggaagaa tcaaaggccg     4560

gtctcgggat ccgtgggtat atatatatat atatatatat acgatccttc gttacctccc     4620

tgttctcaaa actgtggttt ttcgtttttc gttttttgct ttttttgatt tttttagggc     4680

caactaagct tccagatttc gctaatcacc tttgtactaa ttacaagaaa ggaagaagct     4740

gattagagtt gggcttttta tgcaactgtg ctactcctta tctctgatat gaaagtgtag     4800

acccaatcac atcatgtcat ttagagttgg taatactggg aggatagata aggcacgaaa     4860

acgagccata gcagacatgc tgggtgtagc caagcagaag aaagtagatg ggagccaatt     4920

gacgagcgag ggagctacgc caatccgaca tacgacacgc tgagatcgtc ttggccgggg     4980

ggtacctaca gatgtccaag ggtaagtgct tgactgtaat tgtatgtctg aggacaaata     5040

tgtagtcagc cgtataaagt cataccaggc accagtgcca tcatcgaacc actaactctc     5100

tatgatacat gcctccggta ttattgtacc atgcgtcgct ttgttacata cgtatcttgc     5160

ctttttctct cagaaactcc agactttggc tattggtcga gataagcccg gaccatagtg     5220

agtctttcac actctacatt tctcccttgc tccaactatc gattgttgtc tactaactat     5280

cgtacgataa cttcgtatag catacattat acgaagttat cgcgtcgacg agtatctgtc     5340

tgactcgtca ttgccgcctt tggagtacga ctccaactat gagtgtgctt ggatcacttt     5400

gacgatacat tcttcgttgg aggctgtggg tctgacagct gcgttttcgg cgcggttggc     5460

cgacaacaat atcagctgca acgtcattgc tggctttcat catgatcaca tttttgtcgg     5520

caaaggcgac gcccagagag ccattgacgt tctttctaat ttggaccgat agccgtatag     5580

tccagtctat ctataagttc aactaactcg taactattac cataacatat acttcactgc     5640

cccagataag gttccgataa aaagttctgc agactaaatt tatttcagtc tcctcttcac     5700

caccaaaatg ccctcctacg aagctcgagc taacgtccac aagtccgcct ttgccgctcg     5760

agtgctcaag ctcgtggcag ccaagaaaac caacctgtgt gcttctctgg atgttaccac     5820

caccaaggag ctcattgagc ttgccgataa ggtcggacct tatgtgtgca tgatcaaaac     5880

ccatatcgac atcattgacg acttcaccta cgccggcact gtgctccccc tcaaggaact     5940

tgctcttaag cacggtttct tcctgttcga ggacagaaag ttcgcagata ttggcaacac     6000

tgtcaagcac cagtaccggt gtcaccgaat cgccgagtgg tccgatatca ccaacgccca     6060

cggtgtaccc ggaaccggaa tcattgctgg cctgcgagct ggtgccgagg aaactgtctc     6120

tgaacagaag aaggaggacg tctctgacta cgagaactcc cagtacaagg agttcctagt     6180

cccctctccc aacgagaagc tggccagagg tctgctcatg ctggccgagc tgtcttgcaa     6240

gggctctctg gccactggcg agtactccaa gcagaccatt gagcttgccc gatccgaccc     6300

cgagtttgtg gttggcttca ttgcccagaa ccgacctaag ggcgactctg aggactggct     6360

tattctgacc cccggggtgg gtcttgacga caagggagac gctctcggac agcagtaccg     6420

aactgttgag gatgtcatgt ctaccggaac ggatatcata attgtcggcc gaggtctgta     6480

cggccagaac cgagatccta ttgaggaggc caagcgatac cagaaggctg gctgggaggc     6540

ttaccagaag attaactgtt agaggttaga ctatggatat gtaatttaac tgtgtatata     6600

gagagcgtgc aagtatggag cgcttgttca gcttgtatga tggtcagacg acctgtctga     6660

tcgagtatgt atgatactgc acaacctgtg tatccgcatg atctgtccaa tggggcatgt     6720

tgttgtgttt ctcgatacgg agatgctggg tacagtgcta atacgttgaa ctacttatac     6780

ttatatgagg ctcgaagaaa gctgacttgt gtatgactta ttctcaacta catccccagt     6840

cacaatacca ccactgcact accactacac caaaaccatg atcaaaccac ccatggactt     6900

cctggaggca gaagaacttg ttatggaaaa gctcaagaga gagatcataa cttcgtatag     6960

catacattat acgaagttat cctgcaggta aaggaattca tgctgttcat cgtggttaat     7020

gctgctgtgt gctgtgtgtg tgtgttgttt ggcgctcatt gttgcgttat gcagcgtaca     7080

ccacaatatt ggaagcttat tagcctttct attttttcgt ttgcaaggct taacaacatt     7140

gctgtggaga gggatgggga tatggaggcc gctggaggga gtcggagagg cgttttggag     7200

cggcttggcc tggcgcccag ctcgcgaaac gcacctagga ccctttggca cgccgaaatg     7260

tgccactttt cagtctagta acgccttacc tacgtcattc catgcgtgca tgtttgcgcc     7320

ttttttccct tgcccttgat cgccacacag tacagtgcac tgtacagtgg aggttttggg     7380

ggggtcttag atgggagcta aaagcggcct agcggtacac tagtgggatt gtatggagtg     7440

gcatggagcc taggtggagc ctgacaggac gcacgaccgg ctagcccgtg acagacgatg     7500

ggtggctcct gttgtccacc gcgtacaaat gtttgggcca aagtcttgtc agccttgctt     7560

gcgaacctaa ttcccaattt tgtcacttcg cacccccatt gatcgagccc taacccctgc     7620

ccatcaggca atccaattaa gctcgcattg tctgccttgt ttagtttggc tcctgcccgt     7680

ttcggcgtcc acttgcacaa acacaaacaa gcattatata taaggctcgt ctctccctcc     7740

caaccacact cacttttttg cccgtcttcc cttgctaaca caaaagtcaa gaacacaaac     7800

aaccacccca acccccttac acacaagaca tatctacagc aatggccatg gcttcttcca     7860

ctgttgctgc gccgtacgag ttcccgacgc tgacggagat caagcgctcg ctgccagcgc     7920

actgctttga ggcctcggtc ccgtggtcgc tctactacac cgtgcgcgcg ctgggcatcg     7980

ccggctcgct cgcgctcggc ctctactacg cgcgcgcgct cgcgatcgtg caggagtttg     8040

ccctgctgga tgcggtgctc tgcacggggt acattctgct gcagggcatc gtattctggg     8100

ggttcttcac catcggccat gactgcggcc acggcgcgtt ctcgcgttcg cacctgctca     8160

acttcagcgt cggcacgctc attcactcga tcatcctcac gccgtacgag tcatggaaga     8220

tctcgcaccg ccaccaccac aagaacacgg gcaacatcga caaggacgag attttctacc     8280

cgcagcgcga ggccgactcg cacccactgt cccgacacat ggtgatctcg ctcggctcgg     8340

cctggttcgc gtacctcgtt gcgggcttcc ctcctcgcaa ggtgaaccac ttcaaccctt     8400

gggaaccgtt gtacctgcgc cgcatgtctg ccgtcatcat ctcactcggc tcgctcgtgg     8460

cgttcgcggg cttgtatgcg tatctcacct acgtctatgg ccttaagacc atggcgctgt     8520

actacttcgc ccctctcttt gggttcgcca cgatgctcgt ggtcactacc tttttgcacc     8580

acaatgacga ggaaacgcca tggtacgccg actcggagtg gacgtacgtc aagggcaacc     8640

tctcgtccgt ggaccgctcg tacggcgcgc tcatcgacaa cctgagccac aacatcggca     8700

cgcaccagat ccaccacctg tttccgatca tcccgcacta caagctgaac gaggcgacgg     8760

cagcgttcgc gcaggcgttc ccggagctcg tgcgcaagag cgcgtcgccg atcatcccga     8820

cgttcatccg catcgggctc atgtacgcca agtacggcgt cgtggacaag gacgccaaga     8880

tgtttacgct caaggaggcc aaggccgcca agaccaaggc caactaggcg gccgcattga     8940

tgattggaaa cacacacatg ggttatatct aggtgagagt tagttggaca gttatatatt     9000

aaatcagcta tgccaacggt aacttcattc atgtcaacga ggaaccagtg actgcaagta     9060

atatagaatt tgaccacctt gccattctct tgcactcctt tactatatct catttatttc     9120

ttatatacaa atcacttctt cttcccagca tcgagctcgg aaacctcatg agcaataaca     9180

tcgtggatct cgtcaataga gggctttttg gactccttgc tgttggccac cttgtccttg     9240

ctgtttaaac agtgtacgca gatctactat agaggaacat ttaaattgcc ccggagaaga     9300

cggccaggcc gcctagatga caaattcaac aactcacagc tgactttctg ccattgccac     9360

tagggggggg cctttttata tggccaagcc aagctctcca cgtcggttgg gctgcaccca     9420

acaataaatg ggtagggttg caccaacaaa gggatgggat ggggggtaga agatacgagg     9480

ataacggggc tcaatggcac aaataagaac gaatactgcc attaagactc gtgatccagc     9540

gactgacacc attgcatcat ctaagggcct caaaactacc tcggaactgc tgcgctgatc     9600

tggacaccac agaggttccg agcactttag gttgcaccaa atgtcccacc aggtgcaggc     9660

agaaaacgct ggaacagcgt gtacagtttg tcttaacaaa aagtgagggc gctgaggtcg     9720

agcagggtgg tgtgacttgt tatagccttt agagctgcga aagcgcgtat ggatttggct     9780

catcaggcca gattgagggt ctgtggacac atgtcatgtt agtgtacttc aatcgccccc     9840

tggatatagc cccgacaata ggccgtggcc tcattttttt gccttccgca catttccatt     9900

gctcggtacc cacaccttgc ttctcctgca cttgccaacc ttaatactgg tttacattga     9960

ccaacatctt acaagcgggg ggcttgtcta gggtatatat aaacagtggc tctcccaatc    10020

ggttgccagt ctcttttttc ctttctttcc ccacagattc gaaatctaaa ctacacatca    10080

cagaattccg agccgtgagt atccacgaca agatcagtgt cgagacgacg cgttttgtgt    10140

aatgacacaa tccgaaagtc gctagcaaca cacactctct acacaaacta acccagctct    10200

ggtaccatgg cttcttccac tgttgctgcg ccgtacgagt tcccgacgct gacggagatc    10260

aagcgctcgc tgccagcgca ctgctttgag gcctcggtcc cgtggtcgct ctactacacc    10320

gtgcgcgcgc tgggcatcgc cggctcgctc gcgctcggcc tctactacgc gcgcgcgctc    10380

gcgatcgtgc aggagtttgc cctgctggat gcggtgctct gcacggggta cattctgctg    10440

cagggcatcg tattctgggg gttcttcacc atcggccatg actgcggcca cggcgcgttc    10500

tcgcgttcgc acctgctcaa cttcagcgtc ggcacgctca ttcactcgat catcctcacg    10560

ccgtacgagt catggaagat ctcgcaccgc caccaccaca agaacacggg caacatcgac    10620

aaggacgaga ttttctaccc gcagcgcgag gccgactcgc acccactgtc ccgacacatg    10680

gtgatctcgc tcggctcggc ctggttcgcg tacctcgttg cgggcttccc tcctcgcaag    10740

gtgaaccact tcaacccttg ggaaccgttg tacctgcgcc gcatgtctgc cgtcatcatc    10800

tcactcggct cgctcgtggc gttcgcgggc ttgtatgcgt atctcaccta cgtctatggc    10860

cttaagacca tggcgctgta ctacttcgcc cctctctttg ggttcgccac gatgctcgtg    10920

gtcactacct ttttgcacca caatgacgag gaaacgccat ggtacgccga ctcggagtgg    10980

acgtacgtca agggcaacct ctcgtccgtg gaccgctcgt acggcgcgct catcgacaac    11040

ctgagccaca acatcggcac gcaccagatc caccacctgt ttccgatcat cccgcactac    11100

aagctgaacg aggcgacggc agcgttcgcg caggcgttcc cggagctcgt gcgcaagagc    11160

gcgtcgccga tcatcccgac gttcatccgc atcgggctca tgtacgccaa gtacggcgtc    11220

gtggacaagg acgccaagat gtttacgctc aaggaggcca aggccgccaa gaccaaggcc    11280

aactaggcgg ccgcatggag cgtgtgttct gagtcgatgt tttctatgga gttgtgagtg    11340

ttagtagaca tgatgggttt atatatgatg aatgaataga tgtgattttg atttgcacga    11400

tggaattgag aactttgtaa acgtacatgg gaatgtatga atgtgggggt tttgtgactg    11460

gataactgac ggtcagtgga cgccgttgtt caaatatcca agagatgcga gaaactttgg    11520

gtcaagtgaa catgtcctct ctgttcaagt aaaccatcaa ctatgggtag tatatttagt    11580

aaggacaaga gttgagattc tttggagtcc tagaaacgta ttttcgcgtt ccaagatcaa    11640

attagtagag taatacgggc acgggaatcc attcatagtc tcaatcctgc aggtgagtta    11700

attaagatga cgacatttgc gagctggacg aggaatagat ggagcgtgtg ttctgagtcg    11760

atgttttcta tggagttgtg agtgttagta gacatgatgg gtttatatat gatgaatgaa    11820

tagatgtgat tttgatttgc acgatggaat tgagaacttt gtaaacgtac atgggaatgt    11880

atgaatgtgg gggttttgtg actggataac tgacggtcag tggacgccgt tgttcaaata    11940

tccaagagat gcgagaaact ttgggtcaag tgaacatgtc ctctctgttc aagtaaacca    12000

tcaactatgg gtagtatatt tagtaaggac aagagttgag attctttgga gtcctagaaa    12060

cgtattttcg cgttccaaga tcaaattagt agagtaatac gggcacggga atccattcat    12120

agtctcaatt ttcccatagg tgtgctacaa ggtgttgaga tgtggtacag taccaccatg    12180

attcgaggta aagagcccag aagtcattga tgaggtcaag aaatacacag atctacagct    12240

caatacaatg aatatcttct ttcatattct tcaggtgaca ccaagggtgt ctattttccc    12300

cagaaatgcg tgaaaaggcg cgtgtgtagc gtggagtatg ggttcggttg gcgtatcctt    12360

catatatcga cgaaatagta gggcaagaga tgacaaaaag tatctatatg tagacagcgt    12420

agaatatgga tttgattggt ataaattcat ttattgcgtg tctcacaaat actctcgata    12480

agttggggtt aaactggaga tggaacaatg tcgatatctc gacgcatgcg acgtcgggcc    12540

caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga    12600

ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag    12660

ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa    12720

tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc    12780

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc    12840

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg    12900

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca    12960

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc    13020

tttaatagtg gactcttgtt ccaaactgga acaacactca acccta                   13066


<210>  130
<211>  4313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKUM

<400>  130
taatcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc       60

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga      120

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg      180

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg      240

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg      300

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga      360

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg      420

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag      480

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc      540

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg      600

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt      660

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc      720

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc      780

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg      840

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca      900

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc      960

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     1020

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     1080

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     1140

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     1200

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc     1260

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata     1320

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg     1380

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc     1440

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct     1500

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa     1560

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt     1620

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca     1680

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac     1740

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca     1800

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt     1860

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc     1920

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca     1980

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata     2040

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc     2100

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     2160

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt     2220

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc     2280

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct     2340

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat     2400

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc     2460

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc     2520

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg     2580

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc     2640

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc     2700

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc     2760

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat     2820

tgggtaccgg gccccccctc gaggtcgacg agtatctgtc tgactcgtca ttgccgcctt     2880

tggagtacga ctccaactat gagtgtgctt ggatcacttt gacgatacat tcttcgttgg     2940

aggctgtggg tctgacagct gcgttttcgg cgcggttggc cgacaacaat atcagctgca     3000

acgtcattgc tggctttcat catgatcaca tttttgtcgg caaaggcgac gcccagagag     3060

ccattgacgt tctttctaat ttggaccgat agccgtatag tccagtctat ctataagttc     3120

aactaactcg taactattac cataacatat acttcactgc cccagataag gttccgataa     3180

aaagttctgc agactaaatt tatttcagtc tcctcttcac caccaaaatg ccctcctacg     3240

aagctcgagt gctcaagctc gtggcagcca agaaaaccaa cctgtgtgct tctctggatg     3300

ttaccaccac caaggagctc attgagcttg ccgataaggt cggaccttat gtgtgcatga     3360

tcaaaaccca tatcgacatc attgacgact tcacctacgc cggcactgtg ctccccctca     3420

aggaacttgc tcttaagcac ggtttcttcc tgttcgagga cagaaagttc gcagatattg     3480

gcaacactgt caagcaccag taccggtgtc accgaatcgc cgagtggtcc gatatcacca     3540

acgcccacgg tgtacccgga accggaatcg attgctggcc tgcgagctgg tgcgtacgag     3600

gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc ccagtacaag     3660

gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat gctggccgag     3720

ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat tgagcttgcc     3780

cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa gggcgactct     3840

gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga cgctctcgga     3900

cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat aattgtcggc     3960

cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata ccagaaggct     4020

ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata tgtaatttaa     4080

ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg atggtcagac     4140

gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat gatctgtcca     4200

atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct aatacgttga     4260

actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt aat            4313


<210>  131
<211>  15991
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL2-5mB89C

<400>  131
gtacgttatc atttgaacag tgaaaggcta cagtaacaga agcagttgta aacttcattc       60

cgttgattct gtactacagt accccactac gccgcttccg ctgacactgt tcaacccaaa      120

aactacatct gcgtgcgctg tgtaaggcta tcatcagata catactgtag attctgtaga      180

tgcgaacctg cttgtatcat atacatcccc ctccccctga cctgcacaag caagcaatgt      240

gacattgata ttgctgctta tctagtgccg aggatgtgaa agccgagact caaacatttc      300

ttttactctc ttgttcctga ccagacctgg cggagattac gccagtatga ttcttgcagg      360

tctgagacaa gcctggaaca gccaacattt atttttcgaa gcgagaaaca tgccacaccc      420

cggcacgttc agagatgcat atgatttgtt tttcgagtaa cagtaccccc cccccccccc      480

ccaatgaaac cagtattact cacaccatcc tcattcaaag cgttacactg attacgcgcc      540

catcaacgac agcatgaggg gactgctgat ctgatctaat caaatgacta caaaaatcgc      600

aataatgaag agcaaacgac aaaaaagaaa caggttaacc aatcccgctt caatgtctca      660

ccacaatcca gcactgtttc tcattacctc ctccctctaa tttcagagtt gcatcagggt      720

ccttgatggc gcgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc      780

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc      840

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata      900

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg      960

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct     1020

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa     1080

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc     1140

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt     1200

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg     1260

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg     1320

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct     1380

tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc     1440

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     1500

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     1560

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     1620

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     1680

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat     1740

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct     1800

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg     1860

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag     1920

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta     1980

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg     2040

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg     2100

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct     2160

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta     2220

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg     2280

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc     2340

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg     2400

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga     2460

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg     2520

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat     2580

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc     2640

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca     2700

catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga     2760

aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt     2820

gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa     2880

aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa     2940

agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac     3000

gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga     3060

accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa     3120

aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc     3180

tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc     3240

attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca     3300

gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca     3360

gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt     3420

gggcccgacg tcgcatgctg gtttcgattt gtcttagagg aacgcatata cagtaatcat     3480

agagaataaa cgatattcat ttattaaagt agatagttga ggtagaagtt gtaaagagtg     3540

ataaatagct tagataccac agacaccctc ggtgacgaag tactgcagat ggtttccaat     3600

cacattgacc tgctggagca gagtgttacc ggcagagcac tgtttattgc tctggccctg     3660

gcacatgaca acgttggaga gaggagggtg gatcaggggc cagtcaataa agacctcacc     3720

agagcagtgc tggtaaccgt cccagaaggg cacttgaggg acgatatctc ctcggtgggt     3780

gattcggtag agctttcggt ctttggacac cttggagaca tcggggttct cctggccaaa     3840

gaagagttta tcgacccagt tagcaaagcc agcgttaccg acaatgggct gaccaagagt     3900

aacaacgagg ggatcgtggc cgttaacctt gaggttgatt ccgaacagaa gggctgcagc     3960

tcctccgaga gagtgaccgg tgacagcaat ctggtagtcg ggatactgct caatcacaga     4020

gtcgagcttg gggccgatct gattgtaggt gttgttgtag gactggatga agccattgtg     4080

gacaagacag tcatcacaag tagcagtaga agagatgtta gcagcaagat caaagttaat     4140

taactcacct gcaggattga gactatgaat ggattcccgt gcccgtatta ctctactaat     4200

ttgatcttgg aacgcgaaaa tacgtttcta ggactccaaa gaatctcaac tcttgtcctt     4260

actaaatata ctacccatag ttgatggttt acttgaacag agaggacatg ttcacttgac     4320

ccaaagtttc tcgcatctct tggatatttg aacaacggcg tccactgacc gtcagttatc     4380

cagtcacaaa acccccacat tcatacattc ccatgtacgt ttacaaagtt ctcaattcca     4440

tcgtgcaaat caaaatcaca tctattcatt catcatatat aaacccatca tgtctactaa     4500

cactcacaac tccatagaaa acatcgactc agaacacacg ctccatgcgg ccgcttagga     4560

atcctgagcg tccttgacac agtgaaccac accgactttg tgcatgtact tgagggtgga     4620

aatgatgttg cccacaatgg tagggtagaa gacgtaccga actccgtgtc gttcgcaaca     4680

ctctcggaca gcttgctgca cgaagggata gtgccaagac gacattcgag gaaagaggtg     4740

atgctcgatc tggaagttga gaccgccagt aaagaacatg gcaatgggtc caccgtaggt     4800

ggaagaggtc tccacctgag ctctgtacca gtcgatctga tcggcttcaa cgtccttctc     4860

ggagctcttg accttgcagt tcttgtcggg gattcgctcc gagccatcga agttgtgaga     4920

caagatgaaa aagaaggtga ggaaggcacc ggtagcagtg ggcaccagag gaatggtgat     4980

gagcagggag gttccagtga gataccaggg caagaaggcg gttcgaaaga tgaagaaagc     5040

tcgcataacg aatgcaaggg ttcggtaccg tcgcagaaag ccgttctctc gcatggctgt     5100

gacagactcg ggaatggtgt cgttgtgctg cattcggaag atgtagagag ggttgtacac     5160

cagcgaaacg ccgtaggctc caagcacgag gtacatgtac caggcctgga atcggtgaaa     5220

ccactttcga gcagtgttgg cagcagggta gttgtggaac acaaggaatg gttctgcgga     5280

ctcggcatcc aggtcgagac catgctgatt ggtgtaggtg tgatgtcgca tgatgtgaga     5340

ctgcagccag atccatctgg acgatccaat gacgtcgatg ccgtaggcaa agagagcgtt     5400

gacccagggc tttttgctga tggcaccatg agaggcatcg tgctgaatgg acaggccgat     5460

ctgcatgtgc atgaatccag tcaagagacc ccacagcacc attccggtag tagcccagtg     5520

ccactcgcaa aaggcggtga cagcaatgat gccaacggtt cgcagccaga atccaggtgt     5580

ggcataccag ttccgacctt tcatgacctc tcgcatagtt cgcttgacgt cctgtgcaaa     5640

gggagagtcg taggtgtaga caatgtcctt ggaggttcgg tcgtgcttgc ctcgcacgaa     5700

ctgttgaagc agcttcgagt tctcgggctt gacgtaaggg tgcatggagt agaacagagg     5760

agaagcatcg gaggcaccag aagcgaggat caagtcggat ccgggatgga ccttggcaag     5820

accttccaga tcgtagagaa tgccgtcgat ggcaaccagg tcgggtcgct cgagcagctg     5880

ctcggtagta agggagagag ccatgggcag gacctgtgtt agtacattgt cggggagtca     5940

tcaattggtt cgacaggttg tcgactgtta gtatgagctc aattgggctc tggtgggtcg     6000

atgacacttg tcatctgttt ctgttgggtc atgtttccat caccttctat ggtactcaca     6060

attcgtccga ttcgcccgaa tccgttaata ccgactttga tggccatgtt gatgtgtgtt     6120

taattcaaga atgaatatag agaagagaag aagaaaaaag attcaattga gccggcgatg     6180

cagaccctta tataaatgtt gccttggaca gacggagcaa gcccgcccaa acctacgttc     6240

ggtataatat gttaagcttt ttaacacaaa ggtttggctt ggggtaacct gatgtggtgc     6300

aaaagaccgg gcgttggcga gccattgcgc gggcgaatgg ggccgtgact cgtctcaaat     6360

tcgagggcgt gcctcaattc gtgcccccgt ggctttttcc cgccgtttcc gccccgtttg     6420

caccactgca gccgcttctt tggttcggac accttgctgc gagctaggtg ccttgtgcta     6480

cttaaaaagt ggcctcccaa caccaacatg acatgagtgc gtgggccaag acacgttggc     6540

ggggtcgcag tcggctcaat ggcccggaaa aaacgctgct ggagctggtt cggacgcagt     6600

ccgccgcggc gtatggatat ccgcaaggtt ccatagcgcc attgccctcc gtcggcgtct     6660

atcccgcaac ctctaaatag agcgggaata taacccaagc ttcttttttt tcctttaaca     6720

cgcacacccc caactatcat gttgctgctg ctgtttgact ctactctgtg gaggggtgct     6780

cccacccaac ccaacctaca ggtggatccg gcgctgtgat tggctgataa gtctcctatc     6840

cggactaatt ctgaccaatg ggacatgcgc gcaggaccca aatgccgcaa ttacgtaacc     6900

ccaacgaaat gcctacccct ctttggagcc cagcggcccc aaatcccccc aagcagcccg     6960

gttctaccgg cttccatctc caagcacaag cagcccggaa ttctctctct tgagcttttc     7020

cataacaagt tcttctgcct ccaggaagtc catgggtggt ttgatcatgg ttttggtgta     7080

gtggtagtgc agtggtggta ttgtgactgg ggatgtagtt gagaataagt catacacaag     7140

tcagctttct tcgagcctca tataagtata agtagttcaa cgtattagca ctgtacccag     7200

catctccgta tcgagaaaca caacaacatg ccccattgga cagatcatgc ggatacacag     7260

gttgtgcagt atcatacata ctcgatcaga caggtcgtct gaccatcata caagctgaac     7320

aagcgctcca tacttgcacg ctctctatat acacagttaa attacatatc catagtctaa     7380

cctctaacag ttaatcttct ggtaagcctc ccagccagcc ttctggtatc gcttggcctc     7440

ctcaatagga tctcggttct ggccgtacag acctcggccg acaattatga tatccgttcc     7500

ggtagacatg acatcctcaa cagttcggta ctgctgtccg agagcgtctc ccttgtcgtc     7560

aagacccacc ccgggggtca gaataagcca gtcctcagag tcgcccttag gtcggttctg     7620

ggcaatgaag ccaaccacaa actcggggtc ggatcgggca agctcaatgg tctgcttgga     7680

gtactcgcca gtggccagag agcccttgca agacagctcg gccagcatga gcagacctct     7740

ggccagcttc tcgttgggag aggggactag gaactccttg tactgggagt tctcgtagtc     7800

agagacgtcc tccttcttct gttcagagac agtttcctcg gcaccagctc gcaggccagc     7860

aatgattccg gttccgggta caccgtgggc gttggtgata tcggaccact cggcgattcg     7920

gtgacaccgg tactggtgct tgacagtgtt gccaatatct gcgaactttc tgtcctcgaa     7980

caggaagaaa ccgtgcttaa gagcaagttc cttgaggggg agcacagtgc cggcgtaggt     8040

gaagtcgtca atgatgtcga tatgggtttt gatcatgcac acataaggtc cgaccttatc     8100

ggcaagctca atgagctcct tggtggtggt aacatccaga gaagcacaca ggttggtttt     8160

cttggctgcc acgagcttga gcactcgagc ggcaaaggcg gacttgtgga cgttagctcg     8220

agcttcgtag gagggcattt tggtggtgaa gaggagactg aaataaattt agtctgcaga     8280

actttttatc ggaaccttat ctggggcagt gaagtatatg ttatggtaat agttacgagt     8340

tagttgaact tatagataga ctggactata cggctatcgg tccaaattag aaagaacgtc     8400

aatggctctc tgggcgtcgc ctttgccgac aaaaatgtga tcatgatgaa agccagcaat     8460

gacgttgcag ctgatattgt tgtcggccaa ccgcgccgaa aacgcagctg tcagacccac     8520

agcctccaac gaagaatgta tcgtcaaagt gatccaagca cactcatagt tggagtcgta     8580

ctccaaaggc ggcaatgacg agtcagacag atactcgtcg accttttcct tgggaaccac     8640

caccgtcagc ccttctgact cacgtattgt agccaccgac acaggcaaca gtccgtggat     8700

agcagaatat gtcttgtcgg tccatttctc accaacttta ggcgtcaagt gaatgttgca     8760

gaagaagtat gtgccttcat tgagaatcgg tgttgctgat ttcaataaag tcttgagatc     8820

agtttggcca gtcatgttgt ggggggtaat tggattgagt tatcgcctac agtctgtaca     8880

ggtatactcg ctgcccactt tatacttttt gattccgctg cacttgaagc aatgtcgttt     8940

accaaaagtg agaatgctcc acagaacaca ccccagggta tggttgagca aaaaataaac     9000

actccgatac ggggaatcga accccggtct ccacggttct caagaagtat tcttgatgag     9060

agcgtatcga tcgaggaaga ggacaagcgg ctgcttctta agtttgtgac atcagtatcc     9120

aaggcaccat tgcaaggatt caaggctttg aacccgtcat ttgccattcg taacgctggt     9180

agacaggttg atcggttccc tacggcctcc acctgtgtca atcttctcaa gctgcctgac     9240

tatcaggaca ttgatcaact tcggaagaaa cttttgtatg ccattcgatc acatgctggt     9300

ttcgatttgt cttagaggaa cgcatataca gtaatcatag agaataaacg atattcattt     9360

attaaagtag atagttgagg tagaagttgt aaagagtgat aaatagcggc cgctcactga     9420

atctttttgg ctcccttgtg ctttcggacg atgtaggtct gcacgtagaa gttgaggaac     9480

agacacagga cagtaccaac gtagaagtag ttgaaaaacc agccaaacat tctcattcca     9540

tcttgtcggt agcagggaat gttccggtac ttccagacga tgtagaagcc aacgttgaac     9600

tgaatgatct gcatagaagt aatcagggac ttgggcatag ggaacttgag cttgatcagt     9660

cgggtccaat agtagccgta catgatccag tgaatgaagc cgttgagcag cacaaagatc     9720

caaacggctt cgtttcggta gttgtagaac agccacatgt ccataggagc tccgagatgg     9780

tgaaagaact gcaaccaggt cagaggcttg cccatgaggg gcagatagaa ggagtcaatg     9840

tactcgagga acttgctgag gtagaacagc tgagtggtga ttcggaagac attgttgtcg     9900

aaagccttct cgcagttgtc ggacatgaca ccaatggtgt acatggcgta ggccatagag     9960

aggaaggagc ccagcgagta gatggacatg agcaggttgt agttggtgaa cacaaacttc    10020

attcgagact gacccttggg tccgagagga ccaagggtga acttcaggat gacgaaggcg    10080

atggagaggt acagcacctc gcagtgcgag gcatcagacc agagctgagc atagtcgacc    10140

ttgggaagaa cctcctggcc aatggagacg atttcgttca cgacctccat ggttgtgaat    10200

tagggtggtg agaatggttg gttgtaggga agaatcaaag gccggtctcg ggatccgtgg    10260

gtatatatat atatatatat atatacgatc cttcgttacc tccctgttct caaaactgtg    10320

gtttttcgtt tttcgttttt tgcttttttt gattttttta gggccaacta agcttccaga    10380

tttcgctaat cacctttgta ctaattacaa gaaaggaaga agctgattag agttgggctt    10440

tttatgcaac tgtgctactc cttatctctg atatgaaagt gtagacccaa tcacatcatg    10500

tcatttagag ttggtaatac tgggaggata gataaggcac gaaaacgagc catagcagac    10560

atgctgggtg tagccaagca gaagaaagta gatgggagcc aattgacgag cgagggagct    10620

acgccaatcc gacatacgac acgctgagat cgtcttggcc ggggggtacc tacagatgtc    10680

caagggtaag tgcttgactg taattgtatg tctgaggaca aatatgtagt cagccgtata    10740

aagtcatacc aggcaccagt gccatcatcg aaccactaac tctctatgat acatgcctcc    10800

ggtattattg taccatgcgt cgctttgtta catacgtatc ttgccttttt ctctcagaaa    10860

ctccagactt tggctattgg tcgagataag cccggaccat agtgagtctt tcacactctg    10920

tttaaacacc actaaaaccc cacaaaatat atcttaccga atatacagat ctactataga    10980

ggaacaattg ccccggagaa gacggccagg ccgcctagat gacaaattca acaactcaca    11040

gctgactttc tgccattgcc actagggggg ggccttttta tatggccaag ccaagctctc    11100

cacgtcggtt gggctgcacc caacaataaa tgggtagggt tgcaccaaca aagggatggg    11160

atggggggta gaagatacga ggataacggg gctcaatggc acaaataaga acgaatactg    11220

ccattaagac tcgtgatcca gcgactgaca ccattgcatc atctaagggc ctcaaaacta    11280

cctcggaact gctgcgctga tctggacacc acagaggttc cgagcacttt aggttgcacc    11340

aaatgtccca ccaggtgcag gcagaaaacg ctggaacagc gtgtacagtt tgtcttaaca    11400

aaaagtgagg gcgctgaggt cgagcagggt ggtgtgactt gttatagcct ttagagctgc    11460

gaaagcgcgt atggatttgg ctcatcaggc cagattgagg gtctgtggac acatgtcatg    11520

ttagtgtact tcaatcgccc cctggatata gccccgacaa taggccgtgg cctcattttt    11580

ttgccttccg cacatttcca ttgctcggta cccacacctt gcttctcctg cacttgccaa    11640

ccttaatact ggtttacatt gaccaacatc ttacaagcgg ggggcttgtc tagggtatat    11700

ataaacagtg gctctcccaa tcggttgcca gtctcttttt tcctttcttt ccccacagat    11760

tcgaaatcta aactacacat cacacaatgc ctgttactga cgtccttaag cgaaagtccg    11820

gtgtcatcgt cggcgacgat gtccgagccg tgagtatcca cgacaagatc agtgtcgaga    11880

cgacgcgttt tgtgtaatga cacaatccga aagtcgctag caacacacac tctctacaca    11940

aactaaccca gctctccatg gtgaaggctt ctcgacaggc tctgcccctc gtcatcgacg    12000

gaaaggtgta cgacgtctcc gcttgggtga acttccaccc tggtggagct gaaatcattg    12060

agaactacca gggacgagat gctactgacg ccttcatggt tatgcactct caggaagcct    12120

tcgacaagct caagcgaatg cccaagatca accaggcttc cgagctgcct ccccaggctg    12180

ccgtcaacga agctcaggag gatttccgaa agctccgaga agagctgatc gccactggca    12240

tgtttgacgc ctctcccctc tggtactcgt acaagatctt gaccaccctg ggtcttggcg    12300

tgcttgcctt cttcatgctg gtccagtacc acctgtactt cattggtgct ctcgtgctcg    12360

gtatgcacta ccagcaaatg ggatggctgt ctcatgacat ctgccaccac cagaccttca    12420

agaaccgaaa ctggaataac gtcctgggtc tggtctttgg caacggactc cagggcttct    12480

ccgtgacctg gtggaaggac agacacaacg cccatcattc tgctaccaac gttcagggtc    12540

acgatcccga cattgataac ctgcctctgc tcgcctggtc cgaggacgat gtcactcgag    12600

cttctcccat ctcccgaaag ctcattcagt tccaacagta ctatttcctg gtcatctgta    12660

ttctcctgcg attcatctgg tgtttccagt ctgtgctgac cgttcgatcc ctcaaggacc    12720

gagacaacca gttctaccga tctcagtaca agaaagaggc cattggactc gctctgcact    12780

ggactctcaa gaccctgttc cacctcttct ttatgccctc catcctgacc tcgatgctgg    12840

tgttctttgt ttccgagctc gtcggtggct tcggaattgc catcgtggtc ttcatgaacc    12900

actaccctct ggagaagatc ggtgattccg tctgggacgg acatggcttc tctgtgggtc    12960

agatccatga gaccatgaac attcgacgag gcatcattac tgactggttc tttggaggcc    13020

tgaactacca gatcgagcac catctctggc ccaccctgcc tcgacacaac ctcactgccg    13080

tttcctacca ggtggaacag ctgtgccaga agcacaacct cccctaccga aaccctctgc    13140

cccatgaagg tctcgtcatc ctgctccgat acctgtccca gttcgctcga atggccgaga    13200

agcagcccgg tgccaaggct cagtaagcgg ccgcatgaga agataaatat ataaatacat    13260

tgagatatta aatgcgctag attagagagc ctcatactgc tcggagagaa gccaagacga    13320

gtactcaaag gggattacac catccatatc cacagacaca agctggggaa aggttctata    13380

tacactttcc ggaataccgt agtttccgat gttatcaatg ggggcagcca ggatttcagg    13440

cacttcggtg tctcggggtg aaatggcgtt cttggcctcc atcaagtcgt accatgtctt    13500

catttgcctg tcaaagtaaa acagaagcag atgaagaatg aacttgaagt gaaggaattt    13560

aaatagttgg agcaagggag aaatgtagag tgtgaaagac tcactatggt ccgggcttat    13620

ctcgaccaat agccaaagtc tggagtttct gagagaaaaa ggcaagatac gtatgtaaca    13680

aagcgacgca tggtacaata ataccggagg catgtatcat agagagttag tggttcgatg    13740

atggcactgg tgcctggtat gactttatac ggctgactac atatttgtcc tcagacatac    13800

aattacagtc aagcacttac ccttggacat ctgtaggtac cccccggcca agacgatctc    13860

agcgtgtcgt atgtcggatt ggcgtagctc cctcgctcgt caattggctc ccatctactt    13920

tcttctgctt ggctacaccc agcatgtctg ctatggctcg ttttcgtgcc ttatctatcc    13980

tcccagtatt accaactcta aatgacatga tgtgattggg tctacacttt catatcagag    14040

ataaggagta gcacagttgc ataaaaagcc caactctaat cagcttcttc ctttcttgta    14100

attagtacaa aggtgattag cgaaatctgg aagcttagtt ggccctaaaa aaatcaaaaa    14160

aagcaaaaaa cgaaaaacga aaaaccacag ttttgagaac agggaggtaa cgaaggatcg    14220

tatatatata tatatatata tatacccacg gatcccgaga ccggcctttg attcttccct    14280

acaaccaacc attctcacca ccctaattca caaccatggg cgtattcatt aaacaggagc    14340

agcttccggc tctcaagaag tacaagtact ccgccgagga tcactcgttc atctccaaca    14400

acattctgcg ccccttctgg cgacagtttg tcaaaatctt ccctctgtgg atggccccca    14460

acatggtgac tctgctgggc ttcttctttg tcattgtgaa cttcatcacc atgctcattg    14520

ttgatcccac ccacgaccgc gagcctccca gatgggtcta cctcacctac gctctgggtc    14580

tgttccttta ccagacattt gatgcctgtg acggatccca tgcccgacga actggccaga    14640

gtggacccct tggagagctg tttgaccact gtgtcgacgc catgaatacc tctctgattc    14700

tcacggtggt ggtgtccacc acccatatgg gatataacat gaagctactg attgtgcaga    14760

ttgccgctct cggaaacttc tacctgtcga cctgggagac ctaccatacc ggaactctgt    14820

acctttctgg cttctctggt cctgttgaag gtatcttgat tctggtggct cttttcgtcc    14880

tcaccttctt cactggtccc aacgtgtacg ctctgaccgt ctacgaggct cttcccgagt    14940

ccatcacttc gctgctgcct gccagcttcc tggacgtcac catcacccag atctacattg    15000

gattcggagt gctgggcatg gtgttcaaca tctacggcgc ctgcggaaac gtgatcaagt    15060

actacaacaa caagggcaag agcgctctcc ccgccattct cggaatcgcc ccctttggca    15120

tcttctacgt cggcgtcttt gcctgggccc atgttgctcc tctgcttctc tccaagtacg    15180

ccatcgtcta tctgtttgcc attggggctg cctttgccat gcaagtcggc cagatgattc    15240

ttgcccatct cgtgcttgct ccctttcccc actggaacgt gctgctcttc ttcccctttg    15300

tgggactggc agtgcactac attgcacccg tgtttggctg ggacgccgat atcgtgtcgg    15360

ttaacactct cttcacctgt tttggcgcca ccctctccat ttacgccttc tttgtgcttg    15420

agatcatcga cgagatcacc aactacctcg atatctggtg tctgcgaatc aagtaccctc    15480

aggagaagaa gaccgaataa gcggccgcat ggagcgtgtg ttctgagtcg atgttttcta    15540

tggagttgtg agtgttagta gacatgatgg gtttatatat gatgaatgaa tagatgtgat    15600

tttgatttgc acgatggaat tgagaacttt gtaaacgtac atgggaatgt atgaatgtgg    15660

gggttttgtg actggataac tgacggtcag tggacgccgt tgttcaaata tccaagagat    15720

gcgagaaact ttgggtcaag tgaacatgtc ctctctgttc aagtaaacca tcaactatgg    15780

gtagtatatt tagtaaggac aagagttgag attctttgga gtcctagaaa cgtattttcg    15840

cgttccaaga tcaaattagt agagtaatac gggcacggga atccattcat agtctcaatc    15900

ctgcaggtga gttaattaat cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg    15960

aaattgttat ccgctcacaa ttccacacaa c                                   15991


<210>  132
<211>  14554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL1-2SR9G85

<400>  132
cgatagttgg agcaagggag aaatgtagag tgtgaaagac tcactatggt ccgggcttat       60

ctcgaccaat agccaaagtc tggagtttct gagagaaaaa ggcaagatac gtatgtaaca      120

aagcgacgca tggtacaata ataccggagg catgtatcat agagagttag tggttcgatg      180

atggcactgg tgcctggtat gactttatac ggctgactac atatttgtcc tcagacatac      240

aattacagtc aagcacttac ccttggacat ctgtaggtac cccccggcca agacgatctc      300

agcgtgtcgt atgtcggatt ggcgtagctc cctcgctcgt caattggctc ccatctactt      360

tcttctgctt ggctacaccc agcatgtctg ctatggctcg ttttcgtgcc ttatctatcc      420

tcccagtatt accaactcta aatgacatga tgtgattggg tctacacttt catatcagag      480

ataaggagta gcacagttgc ataaaaagcc caactctaat cagcttcttc ctttcttgta      540

attagtacaa aggtgattag cgaaatctgg aagcttagtt ggccctaaaa aaatcaaaaa      600

aagcaaaaaa cgaaaaacga aaaaccacag ttttgagaac agggaggtaa cgaaggatcg      660

tatatatata tatatatata tatacccacg gatcccgaga ccggcctttg attcttccct      720

acaaccaacc attctcacca ccctaattca caaccatggc tgccgtcatc gaggtggcca      780

acgagttcgt cgctatcact gccgagaccc ttcccaaggt ggactatcag cgactctggc      840

gagacatcta ctcctgcgag ctcctgtact tctccattgc tttcgtcatc ctcaagttta      900

cccttggcga gctctcggat tctggcaaaa agattctgcg agtgctgttc aagtggtaca      960

acctcttcat gtccgtcttt tcgctggtgt ccttcctctg tatgggttac gccatctaca     1020

ccgttggact gtactccaac gaatgcgaca gagctttcga caacagcttg ttccgatttg     1080

ccaccaaggt cttctactat tccaagtttc tggagtacat cgactctttc taccttcccc     1140

tcatggccaa gcctctgtcc tttctgcagt tctttcatca cttgggagct cctatggaca     1200

tgtggctctt cgtgcagtac tctggcgaat ccatttggat ctttgtgttc ctgaacggat     1260

tcattcactt tgtcatgtac ggctactatt ggacacggct gatgaagttc aactttccca     1320

tgcccaagca gctcattacc gcaatgcaga tcacccagtt caacgttggc ttctacctcg     1380

tgtggtggta caaggacatt ccctgttacc gaaaggatcc catgcgaatg ctggcctgga     1440

tcttcaacta ctggtacgtc ggtaccgttc ttctgctctt catcaacttc tttgtcaagt     1500

cctacgtgtt tcccaagcct aagactgccg acaaaaaggt ccagggcgcc ggtcccgctc     1560

gacctgccgg acttcctccc gctacctact acgactctct ggccgtcatg ggatccgtga     1620

aggcttctcg acaggctctg cccctcgtca tcgacggaaa ggtgtacgac gtctccgctt     1680

gggtgaactt ccaccctggt ggagctgaaa tcattgagaa ctaccaggga cgagatgcta     1740

ctgacgcctt catggttatg cactctcagg aagccttcga caagctcaag cgaatgccca     1800

agatcaacca ggcttccgag ctgcctcccc aggctgccgt caacgaagct caggaggatt     1860

tccgaaagct ccgagaagag ctgatcgcca ctggcatgtt tgacgcctct cccctctggt     1920

actcgtacaa gatcttgacc accctgggtc ttggcgtgct tgccttcttc atgctggtcc     1980

agtaccacct gtacttcatt ggtgctctcg tgctcggtat gcactaccag caaatgggat     2040

ggctgtctca tgacatctgc caccaccaga ccttcaagaa ccgaaactgg aataacgtcc     2100

tgggtctggt ctttggcaac ggactccagg gcttctccgt gacctggtgg aaggacagac     2160

acaacgccca tcattctgct accaacgttc agggtcacga tcccgacatt gataacctgc     2220

ctctgctcgc ctggtccgag gacgatgtca ctcgagcttc tcccatctcc cgaaagctca     2280

ttcagttcca acagtactat ttcctggtca tctgtattct cctgcgattc atctggtgtt     2340

tccagtctgt gctgaccgtt cgatccctca aggaccgaga caaccagttc taccgatctc     2400

agtacaagaa agaggccatt ggactcgctc tgcactggac tctcaagacc ctgttccacc     2460

tcttctttat gccctccatc ctgacctcga tgctggtgtt ctttgtttcc gagctcgtcg     2520

gtggcttcgg aattgccatc gtggtcttca tgaaccacta ccctctggag aagatcggtg     2580

attccgtctg ggacggacat ggcttctctg tgggtcagat ccatgagacc atgaacattc     2640

gacgaggcat cattactgac tggttctttg gaggcctgaa ctaccagatc gagcaccatc     2700

tctggcccac cctgcctcga cacaacctca ctgccgtttc ctaccaggtg gaacagctgt     2760

gccagaagca caacctcccc taccgaaacc ctctgcccca tgaaggtctc gtcatcctgc     2820

tccgatacct gtcccagttc gctcgaatgg ccgagaagca gcccggtgcc aaggctcagt     2880

aagcggccgc atgagaagat aaatatataa atacattgag atattaaatg cgctagatta     2940

gagagcctca tactgctcgg agagaagcca agacgagtac tcaaagggga ttacaccatc     3000

catatccaca gacacaagct ggggaaaggt tctatataca ctttccggaa taccgtagtt     3060

tccgatgtta tcaatggggg cagccaggat ttcaggcact tcggtgtctc ggggtgaaat     3120

ggcgttcttg gcctccatca agtcgtacca tgtcttcatt tgcctgtcaa agtaaaacag     3180

aagcagatga agaatgaact tgaagtgaag gaatttaaat gtaacgaaac tgaaatttga     3240

ccagatattg tgtccgcggt ggagctccag cttttgttcc ctttagtgag ggttaatttc     3300

gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaag     3360

cttccacaca acgtacgata gttagtagac aacaatcaga acatctccct ccttatataa     3420

tcacacaggc cagaacgcgc taaactaaag cgctttggac actatgttac attggcattg     3480

attgaactga aaccacagtc tccctcgcct gaatcgagca atggatgttg tcggaagtca     3540

acttcactag aagagcggtt ctatgccttg tcaagatcat atcataaact cactctgtat     3600

taccccatct atagaacact tgttatgaat gggcggaaac attccgctat atgcaccttt     3660

ccacactaat gcaaagatgt gcatcttcaa cgggtagtaa gactggttcc gacttccgtt     3720

gcatggagag caatgacctc gataatgcga acatccccca catatacact cttacacagg     3780

ccaatataat ctgtgcattt actaaatatt taagtctatg cacctgcttg atgaaaagcg     3840

gcacggatgg tatcatctag tttccgccaa tccaagaacc aactgtgttg gcagtggtgt     3900

agcccatggc acacagacca aagatgaaaa tacagacatc ggcggttcga gccgtggtgc     3960

ctcgagcaac acccttgtaa tgcaaaagag gagggtaaat gtacaccaga ggcacacatg     4020

caaacgatcc ggtgagagcg acgaaccgat cgagatcgtc ggcacctccc catgcaacaa     4080

aggcggtgac aaacacaagg aagaaccgga aaatgttctt ctgccacttg atggtagagt     4140

tgtacttgcc tgatcgggtg aagagaccat tctcgatgat tcggatggcg cgccagctgc     4200

attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt     4260

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     4320

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     4380

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     4440

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     4500

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     4560

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     4620

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     4680

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     4740

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     4800

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     4860

gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     4920

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     4980

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     5040

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat     5100

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct     5160

aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta     5220

tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa     5280

ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac     5340

gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa     5400

gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag     5460

taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg     5520

tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag     5580

ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg     5640

tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc     5700

ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat     5760

tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata     5820

ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa     5880

aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca     5940

actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc     6000

aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc     6060

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg     6120

aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac     6180

ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg     6240

taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta     6300

accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt     6360

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca     6420

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa     6480

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat     6540

ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag     6600

gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg     6660

ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg     6720

ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc     6780

tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac     6840

ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt cgcatgctta     6900

gaagtgagga ttacaagaag cctctggata tcaatgatga acgtactcag cggctggtca     6960

agcatttcga ccgtcgaatc gacgaggtgt tcacctttga caagcgaggg ttcccaattg     7020

atcacgttct cgagttgttc aaatcttctc tcaacatctc tctgcatgaa ctatctctgt     7080

tgacgaacgt gtcacccact gttcctcgaa cgcccttctc cgagtttggt ctgaacatct     7140

tcgatctcaa actgaccccc gcagtgatca atagtgccat gccactgccg atgcggtgcg     7200

aacatccctg gagggattct cggagctcta cacaatgcag attctgtcgt cgagtactct     7260

ctaccttgct cgaatgactt attgtgctac tactgcactc atgcttcgat catgtgccct     7320

actgcacccc aaatttggtg atctgattga gacagagtac cctcttcagc tgattcagaa     7380

gatcatcagc aacatgaatg atgtggttga ccaggcaggc tgttgtagtc acgtccttca     7440

cttcaagttc attcttcatc tgcttctgtt ttactttgac aggcaaatga agacatggta     7500

cgacttgatg gaggccaaga acgccatttc accccgagac accgaagtgc ctgaaatcct     7560

ggctgccccc attgataaca tcggaaacta cggtattccg gaaagtgtat atagaacctt     7620

tccccagctt gtgtctgtgg atatggatgg tgtaatcccc ttaattaact cacctgcagg     7680

attgagacta tgaatggatt cccgtgcccg tattactcta ctaatttgat cttggaacgc     7740

gaaaatacgt ttctaggact ccaaagaatc tcaactcttg tccttactaa atatactacc     7800

catagttgat ggtttacttg aacagagagg acatgttcac ttgacccaaa gtttctcgca     7860

tctcttggat atttgaacaa cggcgtccac tgaccgtcag ttatccagtc acaaaacccc     7920

cacattcata cattcccatg tacgtttaca aagttctcaa ttccatcgtg caaatcaaaa     7980

tcacatctat tcattcatca tatataaacc catcatgtct actaacactc acaactccat     8040

agaaaacatc gactcagaac acacgctcca tgcggccgct taggcaacgg gcttgatgac     8100

agcgggagga gtgcccacat tgtttcggtt tcgaaagaac aggacaccct tgccagctcc     8160

ctcggcacca gcggagggtt caacccactg gcacattcgt gcagatcggt acatggctcg     8220

aatgaatcct cgaggaccgt cctggacatc agctcgatag tgcttgccca tgataggttt     8280

gatggcctcg gtagcttcgt ccgcattgta gaagggaatg gaagagacgt agtgatgcag     8340

gacgtgagtc tcgataatgc cgtggagcag atgacgtcca atgaagccca tctctcggtc     8400

gatggttgca gcggcacctc gcacaaagtt ccactcgtcg ttggtgtagt ggggaagagt     8460

aggatctgtg tgctgcagaa aggtaatggc gacgagccag tggttaaccc acaagtaggg     8520

aacgaagtac cagatggcca tgttgtagaa tccgaacttc tgaacgagaa agtacagagc     8580

ggtggccata agaccaatgc caatgtcgga gagcacgatg agcttggcgt cgctgttctc     8640

gtacagagga gatcggggat cgaaatggtt aactccaccg ccaagaccgt tgtgctttcc     8700

cttgcctcga ccctctcgct gccgctcatg gtagttgtgt ccagtaacgt tggtaatgag     8760

atagttgggc caaccgacca gttgctgaag cacaagcatg agcagggtga aagcaggagt     8820

ttcctcggta agatgggcga gttcgtgggt catcttgccg agtcgagtag cttgctgctc     8880

tcgggttcga ggaacgaaga ccatgtctcg ctccatgttt ccagtggcct tgtgatgctt     8940

ccggtgggag atttgccagc tgaagtaggg aacaagcagg gaagagtgaa gcacccagcc     9000

agtaatgtcg ttgatgattc gggaatcgga gaaagcacca tgtccacact cgtgggcaat     9060

gacccacagt ccagtaccga agagtccctg aagaacggtg tacacagccc acagaccggc     9120

tcgagcagga gtggagggaa tgtactcggg tgtcacaaag ttgtaccaga tgctgaaagt     9180

ggtagtcagg aggacaatgt ctcgaagaat gtagccgtat cccttgagag cagatcgctt     9240

gaagcagtgc ttgggaatag cgttgtagat gtccttgatg gtgaagtcgg gaacttcgaa     9300

ctggttgccg taggtatcca gcatgacacc gtactcggac ttgggcttgg caatgtccac     9360

ctcggacatg gaagacagcg atgtagagga ggccgagtgt ctgggagaat cggagggaga     9420

gacggcagca gactccgagt cggtcacagt ggtggaagtg acggttcgtc ggagggcagg     9480

gttctgcttg ggcagagccg aggtggaggc catggccatt gctgtagata tgtcttgtgt     9540

gtaagggggt tggggtggtt gtttgtgttc ttgacttttg tgttagcaag ggaagacggg     9600

caaaaaagtg agtgtggttg ggagggagag acgagcctta tatataatgc ttgtttgtgt     9660

ttgtgcaagt ggacgccgaa acgggcagga gccaaactaa acaaggcaga caatgcgagc     9720

ttaattggat tgcctgatgg gcaggggtta gggctcgatc aatgggggtg cgaagtgaca     9780

aaattgggaa ttaggttcgc aagcaaggct gacaagactt tggcccaaac atttgtacgc     9840

ggtggacaac aggagccacc catcgtctgt cacgggctag ccggtcgtgc gtcctgtcag     9900

gctccaccta ggctccatgc cactccatac aatcccacta gtgtaccgct aggccgcttt     9960

tagctcccat ctaagacccc cccaaaacct ccactgtaca gtgcactgta ctgtgtggcg    10020

atcaagggca agggaaaaaa ggcgcaaaca tgcacgcatg gaatgacgta ggtaaggcgt    10080

tactagactg aaaagtggca catttcggcg tgccaaaggg tcctaggtgc gtttcgcgag    10140

ctgggcgcca ggccaagccg ctccaaaacg cctctccgac tccctccagc ggcctccata    10200

tccccatccc tctccacagc aatgttgtta agccttgcaa acgaaaaaat agaaaggcta    10260

ataagcttcc aatattgtgg tgtacgctgc ataacgcaac aatgagcgcc aaacaacaca    10320

cacacacagc acacagcagc attaaccacg atgaacagca tgaattctct ctcttgagct    10380

tttccataac aagttcttct gcctccagga agtccatggg tggtttgatc atggttttgg    10440

tgtagtggta gtgcagtggt ggtattgtga ctggggatgt agttgagaat aagtcataca    10500

caagtcagct ttcttcgagc ctcatataag tataagtagt tcaacgtatt agcactgtac    10560

ccagcatctc cgtatcgaga aacacaacaa catgccccat tggacagatc atgcggatac    10620

acaggttgtg cagtatcata catactcgat cagacaggtc gtctgaccat catacaagct    10680

gaacaagcgc tccatacttg cacgctctct atatacacag ttaaattaca tatccatagt    10740

ctaacctcta acagttaatc ttctggtaag cctcccagcc agccttctgg tatcgcttgg    10800

cctcctcaat aggatctcgg ttctggccgt acagacctcg gccgacaatt atgatatccg    10860

ttccggtaga catgacatcc tcaacagttc ggtactgctg tccgagagcg tctcccttgt    10920

cgtcaagacc caccccgggg gtcagaataa gccagtcctc agagtcgccc ttaggtcggt    10980

tctgggcaat gaagccaacc acaaactcgg ggtcggatcg ggcaagctca atggtctgct    11040

tggagtactc gccagtggcc agagagccct tgcaagacag ctcggccagc atgagcagac    11100

ctctggccag cttctcgttg ggagagggga ctaggaactc cttgtactgg gagttctcgt    11160

agtcagagac gtcctccttc ttctgttcag agacagtttc ctcggcacca gctcgcaggc    11220

cagcaatgat tccggttccg ggtacaccgt gggcgttggt gatatcggac cactcggcga    11280

ttcggtgaca ccggtactgg tgcttgacag tgttgccaat atctgcgaac tttctgtcct    11340

cgaacaggaa gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt    11400

aggtgaagtc gtcaatgatg tcgatatggg ttttgatcat gcacacataa ggtccgacct    11460

tatcggcaag ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg    11520

ttttcttggc tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag    11580

ctcgagcttc gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg    11640

cagaactttt tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac    11700

gagttagttg aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa    11760

cgtcaatggc tctctgggcg tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag    11820

caatgacgtt gcagctgata ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac    11880

ccacagcctc caacgaagaa tgtatcgtca aagtgatcca agcacactca tagttggagt    11940

cgtactccaa aggcggcaat gacgagtcag acagatactc gtcgaccatt aattctcacg    12000

tgacacagat tattaacgtc tcgtaccaac cacagattac gacccattcg cagtcacagt    12060

tcactagggt ttgggttgca tccgttgaga gcggtttgtt tttaaccttc tccatgtgct    12120

cactcaggtt ttgggttcag atcaaatcaa ggcgtgaacc actttgtttg aggacaaatg    12180

tgacacaacc aaccagtgtc aggggcaagt ccgtgacaaa ggggaagata caatgcaatt    12240

actgacagtt acagactgcc tcgatgccct aaccttgccc caaaataaga caactgtcct    12300

cgtttaagcg caaccctatt cagcgtcacg tcataatagc gtttggatag cactagtcta    12360

tgaggagcgt tttatgttgc ggtgagggcg attggtgctc atatgggttc aattgaggtg    12420

gcggaacgag cttagtcttc aattgaggtg cgagcgacac aattgggtgt cacgtggcct    12480

aattgacctc gggtcgtgga gtccccagtt atacagcaac cacgaggtgc atgggtagga    12540

gacgtcacca gacaataggg ttttttttgg actggagagg gttgggcaaa agcgctcaac    12600

gggctgtttg gggagctgtg ggggaggaat tggcgatatt tgtgaggtta acggctccga    12660

tttgcgtgtt ttgtcgctcc tgcatctccc catacccata tcttccctcc ccacctcttt    12720

ccacgataat tttacggatc agcaataagg ttccttctcc tagtttccac gtccatatat    12780

atctatgctg cgtcgtcctt ttcgtgacat caccaaaaca catacaacca tggctctctc    12840

ccttactacc gagcagctgc tcgagcgacc cgacctggtt gccatcgacg gcattctcta    12900

cgatctggaa ggtcttgcca aggtccatcc cggatccgac ttgatcctcg cttctggtgc    12960

ctccgatgct tctcctctgt tctactccat gcacccttac gtcaagcccg agaactcgaa    13020

gctgcttcaa cagttcgtgc gaggcaagca cgaccgaacc tccaaggaca ttgtctacac    13080

ctacgactct ccctttgcac aggacgtcaa gcgaactatg cgagaggtca tgaaaggtcg    13140

gaactggtat gccacacctg gattctggct gcgaaccgtt ggcatcattg ctgtcaccgc    13200

cttttgcgag tggcactggg ctactaccgg aatggtgctg tggggtctct tgactggatt    13260

catgcacatg cagatcggcc tgtccattca gcacgatgcc tctcatggtg ccatcagcaa    13320

aaagccctgg gtcaacgctc tctttgccta cggcatcgac gtcattggat cgtccagatg    13380

gatctggctg cagtctcaca tcatgcgaca tcacacctac accaatcagc atggtctcga    13440

cctggatgcc gagtccgcag aaccattcct tgtgttccac aactaccctg ctgccaacac    13500

tgctcgaaag tggtttcacc gattccaggc ctggtacatg tacctcgtgc ttggagccta    13560

cggcgtttcg ctggtgtaca accctctcta catcttccga atgcagcaca acgacaccat    13620

tcccgagtct gtcacagcca tgcgagagaa cggctttctg cgacggtacc gaacccttgc    13680

attcgttatg cgagctttct tcatctttcg aaccgccttc ttgccctggt atctcactgg    13740

aacctccctg ctcatcacca ttcctctggt gcccactgct accggtgcct tcctcacctt    13800

ctttttcatc ttgtctcaca acttcgatgg ctcggagcga atccccgaca agaactgcaa    13860

ggtcaagagc tccgagaagg acgttgaagc cgatcagatc gactggtaca gagctcaggt    13920

ggagacctct tccacctacg gtggacccat tgccatgttc tttactggcg gtctcaactt    13980

ccagatcgag catcacctct ttcctcgaat gtcgtcttgg cactatccct tcgtgcagca    14040

agctgtccga gagtgttgcg aacgacacgg agttcggtac gtcttctacc ctaccattgt    14100

gggcaacatc atttccaccc tcaagtacat gcacaaagtc ggtgtggttc actgtgtcaa    14160

ggacgctcag gattcctaag cggccgcatg tacatacaag attatttata gaaatgaatc    14220

gcgatcgaac aaagagtacg agtgtacgag taggggatga tgataaaagt ggaagaagtt    14280

ccgcatcttt ggatttatca acgtgtagga cgatacttcc tgtaaaaatg caatgtcttt    14340

accataggtt ctgctgtaga tgttattaac taccattaac atgtctactt gtacagttgc    14400

agaccagttg gagtatagaa tggtacactt accaaaaagt gttgatggtt gtaactacga    14460

tatataaaac tgttgacggg atccccgctg atatgcctaa ggaacaatca aagaggaaga    14520

tattaattca gaatgctagt atacagttag ggat                                14554


<210>  133
<211>  15119
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZSCP-Ma83

<400>  133
gtacgacccc tctcaggcca agcagaaggc tgagtccatc aagaaggcca acgctatcat       60

tgtcttcaac ctcaagaaca aggctggcaa gaccgagtct tggtaccttg acctcaagaa      120

cgacggtgac gtcggcaagg gcaacaagtc ccccaagggt gatgctgaca tccagctcac      180

tctctctgac gaccacttcc agcagctcgt tgagggtaag gctaacgccc agcgactctt      240

catgaccggc aagctcaagg ttaagggcaa cgtcatgaag gctgccgcca ttgagggtat      300

cctcaagaac gctcagaaca acctctaagc gcatcattta ttgattaatt gatgatttac      360

tatattgatt tcgcaactgt agtgtgattg tatgtgatct ggctcgtagg cttcagtaaa      420

tactagacgg gtatcctacg tagttgtatc atacatcgag cctgtggtta cttgtacaat      480

aattcgtaat gtagagatac cccttgatcc attgcctgtt tctaacatac aatgatctcc      540

acgcaataat cccactcttg actaaaagtt gctactcttg cacggttacc tcggcatagt      600

cacgcctctc ttgtctcgtc tcgaacgcac aaagtcaatt gacaacgcca ctcactcgag      660

tgtgccccaa cagggcacca tatcgactaa tttgaggcca actagggtga ttttggatgg      720

aatttgatcg gaaaaaatag ctgcagaaat tcctggagag aaaaattgac cgcatccaca      780

tggtttgacc aaaaaatcgt ctccatctct gtgctcaact ctcctgacga gatatgcgcg      840

cgcaccccca catgatgtga ttgatctcaa caaacttcac ccagaccctt atctttccgg      900

gaaacttact gtataagtgg tcgtgcgaac agaaagtgtg cgcactttag gtgtctagat      960

ccgattgttc tcgttctgat aatgagccag ccccgcgagg caatgttttt tacaattgaa     1020

aacttcgtta accactcaca ttaccgtttt tgccccatat ttaccctctg gtacactccc     1080

tcttgcatac acacacactg cagtgaaaat gcactccgtt agcaccgttg tgattggttc     1140

agggcacgag tttggtggtt taaggcgcaa ctacatcaat atgaaaacag gagacgctga     1200

aaaggggtaa tatcggactg ctgctatgtt gtatgtactg catgacgaat tggtgttatt     1260

caagaccgtg gcacaggttg ctgcggtacg agacctggta gcttctctaa acggcatgtc     1320

taggtggcgc gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt     1380

attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg     1440

cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac     1500

gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg     1560

ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca     1620

agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc     1680

tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc     1740

ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag     1800

gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc     1860

ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca     1920

gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg     1980

aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg     2040

aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct     2100

ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa     2160

gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa     2220

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa     2280

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc     2340

ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga     2400

ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca     2460

atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc     2520

ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat     2580

tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc     2640

attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt     2700

tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc     2760

ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg     2820

gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt     2880

gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg     2940

gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga     3000

aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg     3060

taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg     3120

tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt     3180

tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc     3240

atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca     3300

tttccccgaa aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa     3360

ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt     3420

taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa     3480

gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag     3540

aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt     3600

gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac     3660

cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag     3720

gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg     3780

cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat     3840

tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc     3900

tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt     3960

cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg     4020

gcccgacgtc gcatgcgtca ctaatcaagg atacctacca tgccactatg atgtttgcag     4080

gaggtgtacc tcggcagtca tcaaaaaatg gaactactgg ctttagatct tgttgtatgg     4140

catcgcgcct aaaaaagaaa cccccttcca gcgagctact acaagtagtt gtagttgcgg     4200

gcgttggata ccgaaagtca caagcacatg tcgaagctct catctgaaac accgacagtc     4260

gtctgcaccc cgcaagtctc ggttcgtacc agcaccaatg ttaggcagaa ctatacacaa     4320

gagggcggac gatcacttcg gcgttaggca actgaaggct attttcggct ggtactgtag     4380

gggacagagg aaacgcaagt gattagtaaa tcggataata ggcctgttag tttaccgaaa     4440

tggtggggga ggggttccgt ggatatcttg aagttatgga ggctgatcgt tatttgtggg     4500

gatggatatc attgtatgga catactgtag ctactgtata aacaacggat cttacacctg     4560

cctcttgtat gcccattgct tgatcatcta tcgtgttact gtacatatac aatagatata     4620

gggaagaaaa gccggaagta gagaccatag tctggcagaa gtaacggcct cgggtcgaga     4680

gaactataac aaagtccaac ggcgggtctt agaatagccc caaggatcac acagttccgc     4740

aatccagttt cacatgttcc gttgcatgga cttttgcatg tctactgttg ctacgattcc     4800

cccattgcaa ccacagtttg gggttacccc gcattatatt agcatgatta cgaaagagat     4860

aagtatcata tggaacatgt gaagggtagt atgcaggtcc ggcggagaaa gagaatgacg     4920

ttttcattaa gcgattcgct tggcggcttg tgggggatgt gacgatactt acggtaaaga     4980

ccctgtgtga gagctggtac tcgctcgtta cttcgctgat ctgttgggcc gtcaatcgaa     5040

tctcgtggaa cttgcattct tcttaactgt gtctatacaa gacacctaat gaaacataca     5100

agctaccgaa atcattttac tcgtactgac cggtacggta cttgcacaag tagtgaaact     5160

tccgaaaata gccagcctca tgcatcatcg cttcacccct tctgttgacc tcaaaagcat     5220

tccaacggta aaaaattata acgccgccaa ctggatggtt gtgacggcgt tgaccaccaa     5280

tgtgtggggg ctggcggtag gaccgagctt attcgtccca ataagctctt tggatttgat     5340

tctttggggt gtgtggtaaa attcacatgg ggaagaacac ggtggcagtt tgaggcagag     5400

gcccagcgtg tagttcctag ggcatgaata taccgaactc atggcgcaga attgagctga     5460

atgcgcaaaa agctacagga tcaaccgcgt tagaaatgcc gcaaatgtcc actaattccc     5520

cggactgttc caaatgattc tgtggggata aatctcaaac tgggttaggc tttgtcacgt     5580

ttctttgtgt cgtgtcggtt cgtccggggc aatgtgccca cgcttggctg tctccctaca     5640

cctcggtaaa aactatcaca tgctgcccct ctcgagcaag cattaaatgc atatagtcaa     5700

tctaacgaca tatatatagg tagggtgcat cctccggttt agctccccag aatatctctt     5760

attcattaca caaaaacaac aatgtctctc aaggtcgacg gcttcacttc ttaattaagt     5820

tgcgacacat gtcttgatag tatcttgaat tctctctctt gagcttttcc ataacaagtt     5880

cttctgcctc caggaagtcc atgggtggtt tgatcatggt tttggtgtag tggtagtgca     5940

gtggtggtat tgtgactggg gatgtagttg agaataagtc atacacaagt cagctttctt     6000

cgagcctcat ataagtataa gtagttcaac gtattagcac tgtacccagc atctccgtat     6060

cgagaaacac aacaacatgc cccattggac agatcatgcg gatacacagg ttgtgcagta     6120

tcatacatac tcgatcagac aggtcgtctg accatcatac aagctgaaca agcgctccat     6180

acttgcacgc tctctatata cacagttaaa ttacatatcc atagtctaac ctctaacagt     6240

taatcttctg gtaagcctcc cagccagcct tctggtatcg cttggcctcc tcaataggat     6300

ctcggttctg gccgtacaga cctcggccga caattatgat atccgttccg gtagacatga     6360

catcctcaac agttcggtac tgctgtccga gagcgtctcc cttgtcgtca agacccaccc     6420

cgggggtcag aataagccag tcctcagagt cgcccttagg tcggttctgg gcaatgaagc     6480

caaccacaaa ctcggggtcg gatcgggcaa gctcaatggt ctgcttggag tactcgccag     6540

tggccagaga gcccttgcaa gacagctcgg ccagcatgag cagacctctg gccagcttct     6600

cgttgggaga ggggactagg aactccttgt actgggagtt ctcgtagtca gagacgtcct     6660

ccttcttctg ttcagagaca gtttcctcgg caccagctcg caggccagca atgattccgg     6720

ttccgggtac accgtgggcg ttggtgatat cggaccactc ggcgattcgg tgacaccggt     6780

actggtgctt gacagtgttg ccaatatctg cgaactttct gtcctcgaac aggaagaaac     6840

cgtgcttaag agcaagttcc ttgaggggga gcacagtgcc ggcgtaggtg aagtcgtcaa     6900

tgatgtcgat atgggttttg atcatgcaca cataaggtcc gaccttatcg gcaagctcaa     6960

tgagctcctt ggtggtggta acatccagag aagcacacag gttggttttc ttggctgcca     7020

cgagcttgag cactcgagcg gcaaaggcgg acttgtggac gttagctcga gcttcgtagg     7080

agggcatttt ggtggtgaag aggagactga aataaattta gtctgcagaa ctttttatcg     7140

gaaccttatc tggggcagtg aagtatatgt tatggtaata gttacgagtt agttgaactt     7200

atagatagac tggactatac ggctatcggt ccaaattaga aagaacgtca atggctctct     7260

gggcgtcgcc tttgccgaca aaaatgtgat catgatgaaa gccagcaatg acgttgcagc     7320

tgatattgtt gtcggccaac cgcgccgaaa acgcagctgt cagacccaca gcctccaacg     7380

aagaatgtat cgtcaaagtg atccaagcac actcatagtt ggagtcgtac tccaaaggcg     7440

gcaatgacga gtcagacaga tactcgtcga ccttttcctt gggaaccacc accgtcagcc     7500

cttctgactc acgtattgta gccaccgaca caggcaacag tccgtggata gcagaatatg     7560

tcttgtcggt ccatttctca ccaactttag gcgtcaagtg aatgttgcag aagaagtatg     7620

tgccttcatt gagaatcggt gttgctgatt tcaataaagt cttgagatca gtttggccag     7680

tcatgttgtg gggggtaatt ggattgagtt atcgcctaca gtctgtacag gtatactcgc     7740

tgcccacttt atactttttg attccgctgc acttgaagca atgtcgttta ccaaaagtga     7800

gaatgctcca cagaacacac cccagggtat ggttgagcaa aaaataaaca ctccgatacg     7860

gggaatcgaa ccccggtctc cacggttctc aagaagtatt cttgatgaga gcgtatcgat     7920

ggaagccggt agaaccgggc tgcttgtgct tggagatgga agccggtaga accgggctgc     7980

ttggggggat ttggggccgc tgggctccaa agaggggtag gcatttcgtt ggggttacgt     8040

aattgcggca tttgggtcct gcgcgcatgt cccattggtc agaattagtc cggataggag     8100

acttatcagc caatcacagc gccggatcca cctgtaggtt gggttgggtg ggagcacccc     8160

tccacagagt agagtcaaac agcagcagca acatgatagt tgggggtgtg cgtgttaaag     8220

gaaaaaaaag aagcttgggt tatattcccg ctctatttag aggttgcggg atagacgccg     8280

acggagggca atggcgctat ggaaccttgc ggatatccat acgccgcggc ggactgcgtc     8340

cgaaccagct ccagcagcgt tttttccggg ccattgagcc gactgcgacc ccgccaacgt     8400

gtcttggccc acgcactcat gtcatgttgg tgttgggagg ccacttttta agtagcacaa     8460

ggcacctagc tcgcagcaag gtgtccgaac caaagaagcg gctgcagtgg tgcaaacggg     8520

gcggaaacgg cgggaaaaag ccacgggggc acgaattgag gcacgccctc gaatttgaga     8580

cgagtcacgg ccccattcgc ccgcgcaatg gctcgccaac gcccggtctt ttgcaccaca     8640

tcaggttacc ccaagccaaa cctttgtgtt aaaaagctta acatattata ccgaacgtag     8700

gtttgggcgg gcttgctccg tctgtccaag gcaacattta tataagggtc tgcatcgccg     8760

gctcaattga atcttttttc ttcttctctt ctctatattc attcttgaat taaacacaca     8820

tcaaccatgg tcaagcgacc cgctctgcct ctcaccgtgg acggtgtcac ctacgacgtt     8880

tctgcctggc tcaaccacca tcccggaggt gccgacatta tcgagaacta ccgaggtcgg     8940

gatgctaccg acgtcttcat ggttatgcac tccgagaacg ccgtgtccaa actcagacga     9000

atgcccatca tggaaccttc ctctcccctg actccaacac ctcccaagcc aaactccgac     9060

gaacctcagg aggatttccg aaagctgcga gacgagctca ttgctgcagg catgttcgat     9120

gcctctccca tgtggtacgc ttacaagacc ctgtcgactc tcggactggg tgtccttgcc     9180

gtgctgttga tgacccagtg gcactggtac ctggttggtg ctatcgtcct cggcattcac     9240

tttcaacaga tgggatggct ctcgcacgac atttgccatc accagctgtt caaggaccga     9300

tccatcaaca atgccattgg cctgctcttc ggaaacgtgc ttcagggctt ttctgtcact     9360

tggtggaagg accgacacaa cgctcatcac tccgccacca acgtgcaggg tcacgatccc     9420

gacatcgaca acctgcctct cctggcgtgg tccaaggagg acgtcgagcg agctggcccg     9480

ttttctcgac ggatgatcaa gtaccaacag tattacttct ttttcatctg tgcccttctg     9540

cgattcatct ggtgctttca gtccattcat actgccacgg gtctcaagga tcgaagcaat     9600

cagtactatc gaagacagta cgagaaggag tccgtcggtc tggcactcca ctggggtctc     9660

aaggccttgt tctactattt ctacatgccc tcgtttctca ccggactcat ggtgttcttt     9720

gtctccgagc tgcttggtgg cttcggaatt gccatcgttg tcttcatgaa ccactaccct     9780

ctggagaaga ttcaggactc cgtgtgggat ggtcatggct tctgtgctgg acagattcac     9840

gagaccatga acgttcagcg aggcctcgtc acagactggt ttttcggtgg cctcaactac     9900

cagatcgaac atcacctgtg gcctactctt cccagacaca acctcaccgc tgcctccatc     9960

aaagtggagc agctgtgcaa gaagcacaac ctgccctacc gatctcctcc catgctcgaa    10020

ggtgtcggca ttcttatctc ctacctgggc accttcgctc gaatggttgc caaggcagac    10080

aaggcctaag cggccgcatt gatgattgga aacacacaca tgggttatat ctaggtgaga    10140

gttagttgga cagttatata ttaaatcagc tatgccaacg gtaacttcat tcatgtcaac    10200

gaggaaccag tgactgcaag taatatagaa tttgaccacc ttgccattct cttgcactcc    10260

tttactatat ctcatttatt tcttatatac aaatcacttc ttcttcccag catcgagctc    10320

ggaaacctca tgagcaataa catcgtggat ctcgtcaata gagggctttt tggactcctt    10380

gctgttggcc accttgtcct tgctgtttaa acagagtgtg aaagactcac tatggtccgg    10440

gcttatctcg accaatagcc aaagtctgga gtttctgaga gaaaaaggca agatacgtat    10500

gtaacaaagc gacgcatggt acaataatac cggaggcatg tatcatagag agttagtggt    10560

tcgatgatgg cactggtgcc tggtatgact ttatacggct gactacatat ttgtcctcag    10620

acatacaatt acagtcaagc acttaccctt ggacatctgt aggtaccccc cggccaagac    10680

gatctcagcg tgtcgtatgt cggattggcg tagctccctc gctcgtcaat tggctcccat    10740

ctactttctt ctgcttggct acacccagca tgtctgctat ggctcgtttt cgtgccttat    10800

ctatcctccc agtattacca actctaaatg acatgatgtg attgggtcta cactttcata    10860

tcagagataa ggagtagcac agttgcataa aaagcccaac tctaatcagc ttcttccttt    10920

cttgtaatta gtacaaaggt gattagcgaa atctggaagc ttagttggcc ctaaaaaaat    10980

caaaaaaagc aaaaaacgaa aaacgaaaaa ccacagtttt gagaacaggg aggtaacgaa    11040

ggatcgtata tatatatata tatatatata cccacggatc ccgagaccgg cctttgattc    11100

ttccctacaa ccaaccattc tcaccaccct aattcacaac catggtctcc aaccacctgt    11160

tcgacgccat gcgagctgcc gctcccggag acgcaccttt cattcgaatc gacaacgctc    11220

ggacctggac ttacgatgac gccattgctc tttccggtcg aatcgctgga gctatggacg    11280

cactcggcat tcgacccgga gacagagttg ccgtgcaggt cgagaagtct gccgaggcgt    11340

tgattctcta cctggcctgt cttcgaaccg gagctgtcta cctgcctctc aacactgcct    11400

acaccctggc cgagctcgac tacttcatcg gcgatgccga accgcgtctg gtggtcgttg    11460

ctcccgcagc tcgaggtggc gtggagacaa ttgccaagcg acacggtgct atcgtcgaaa    11520

ccctcgacgc cgatggacga ggctccttgc tggaccttgc tagagatgag cctgccgact    11580

ttgtcgatgc ttcgcgatct gccgacgatc tggctgctat tctctacact tccggtacaa    11640

ccggacgatc gaagggtgcc atgcttactc atggcaatct gctctccaac gctctcacct    11700

tgcgagacta ttggagagtt accgcagacg atcgactcat ccatgccttg ccaatctttc    11760

acactcatgg tctgttcgtt gctacgaacg tcacactgct tgcaggagcc tcgatgtttc    11820

tgctctccaa gttcgatgcc gacgaggtcg tttctctcat gccacaggcc accatgctta    11880

tgggcgtgcc cacattctac gttcgattgc tgcagagtcc tcgactcgag aagggtgctg    11940

tggccagcat cagactgttc atttctggat cagctccctt gcttgccgaa acccacgccg    12000

agtttcatgc tcgtactggt cacgccattc tcgagcgata cggcatgacg gaaaccaaca    12060

tgaatacttc caacccctac gagggcaagc gtattgccgg aaccgttggt tttcctctgc    12120

ccgacgtcac tgtgcgagtc accgatcccg ccaccggtct cgttcttcca cctgaagaga    12180

ctggcatgat cgagatcaag ggacccaacg tcttcaaggg ctattggcga atgcccgaaa    12240

agaccgctgc cgagtttacc gcagacggtt tctttatctc tggagatctc ggcaagatcg    12300

accgagaagg ttacgttcac attgtgggac gaggcaagga cctggtcatt tccggtggct    12360

acaacatcta tcccaaagag gtcgaaggcg agatcgacca gatcgagggt gtggtcgagt    12420

ctgctgtcat tggtgttcct catcccgatt tcggagaagg tgtcaccgct gttgtcgtgt    12480

gcaaacctgg tgccgttctc gacgaaaaga ccatcgtgtc tgctctgcag gaccgtcttg    12540

cccgatacaa gcaacccaag cggattatct ttgccgacga tctgcctcga aacactatgg    12600

gaaaggttca gaagaacatt cttcgacagc aatacgccga tctctacacc agacgataag    12660

cggccgcatg agaagataaa tatataaata cattgagata ttaaatgcgc tagattagag    12720

agcctcatac tgctcggaga gaagccaaga cgagtactca aaggggatta caccatccat    12780

atccacagac acaagctggg gaaaggttct atatacactt tccggaatac cgtagtttcc    12840

gatgttatca atgggggcag ccaggatttc aggcacttcg gtgtctcggg gtgaaatggc    12900

gttcttggcc tccatcaagt cgtaccatgt cttcatttgc ctgtcaaagt aaaacagaag    12960

cagatgaaga atgaacttga agtgaaggaa tttaaatgat gtcgacgcag taggatgtcc    13020

tgcacgggtc tttttgtggg gtgtggagaa aggggtgctt ggagatggaa gccggtagaa    13080

ccgggctgct tgtgcttgga gatggaagcc ggtagaaccg ggctgcttgg ggggatttgg    13140

ggccgctggg ctccaaagag gggtaggcat ttcgttgggg ttacgtaatt gcggcatttg    13200

ggtcctgcgc gcatgtccca ttggtcagaa ttagtccgga taggagactt atcagccaat    13260

cacagcgccg gatccacctg taggttgggt tgggtgggag cacccctcca cagagtagag    13320

tcaaacagca gcagcaacat gatagttggg ggtgtgcgtg ttaaaggaaa aaaaagaagc    13380

ttgggttata ttcccgctct atttagaggt tgcgggatag acgccgacgg agggcaatgg    13440

cgctatggaa ccttgcggat atccatacgc cgcggcggac tgcgtccgaa ccagctccag    13500

cagcgttttt tccgggccat tgagccgact gcgaccccgc caacgtgtct tggcccacgc    13560

actcatgtca tgttggtgtt gggaggccac tttttaagta gcacaaggca cctagctcgc    13620

agcaaggtgt ccgaaccaaa gaagcggctg cagtggtgca aacggggcgg aaacggcggg    13680

aaaaagccac gggggcacga attgaggcac gccctcgaat ttgagacgag tcacggcccc    13740

attcgcccgc gcaatggctc gccaacgccc ggtcttttgc accacatcag gttaccccaa    13800

gccaaacctt tgtgttaaaa agcttaacat attataccga acgtaggttt gggcgggctt    13860

gctccgtctg tccaaggcaa catttatata agggtctgca tcgccggctc aattgaatct    13920

tttttcttct tctcttctct atattcattc ttgaattaaa cacacatcaa ccatggagtc    13980

tggacccatg cctgctggca ttcccttccc tgagtactat gacttcttta tggactggaa    14040

gactcccctg gccatcgctg ccacctacac tgctgccgtc ggtctcttca accccaaggt    14100

tggcaaggtc tcccgagtgg ttgccaagtc ggctaacgca aagcctgccg agcgaaccca    14160

gtccggagct gccatgactg ccttcgtctt tgtgcacaac ctcattctgt gtgtctactc    14220

tggcatcacc ttctactaca tgtttcctgc tatggtcaag aacttccgaa cccacacact    14280

gcacgaagcc tactgcgaca cggatcagtc cctctggaac aacgcacttg gctactgggg    14340

ttacctcttc tacctgtcca agttctacga ggtcattgac accatcatca tcatcctgaa    14400

gggacgacgg tcctcgctgc ttcagaccta ccaccatgct ggagccatga ttaccatgtg    14460

gtctggcatc aactaccaag ccactcccat ttggatcttt gtggtcttca actccttcat    14520

tcacaccatc atgtactgtt actatgcctt cacctctatc ggattccatc ctcctggcaa    14580

aaagtacctg acttcgatgc agattactca gtttctggtc ggtatcacca ttgccgtgtc    14640

ctacctcttc gttcctggct gcatccgaac acccggtgct cagatggctg tctggatcaa    14700

cgtcggctac ctgtttccct tgacctatct gttcgtggac tttgccaagc gaacctactc    14760

caagcgatct gccattgccg ctcagaaaaa ggctcagtaa gcggccgcaa gtgtggatgg    14820

ggaagtgagt gcccggttct gtgtgcacaa ttggcaatcc aagatggatg gattcaacac    14880

agggatatag cgagctacgt ggtggtgcga ggatatagca acggatattt atgtttgaca    14940

cttgagaatg tacgatacaa gcactgtcca agtacaatac taaacatact gtacatactc    15000

atactcgtac ccgggcaacg gtttcacttg agtgcagtgg ctagtgctct tactcgtaca    15060

gtgtgcaata ctgcgtatca tagtctttga tgtatatcgt attcattcat gttagttgc     15119


<210>  134
<211>  14623
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL4-398F2


<220>
<221>  misc_feature
<222>  (1048)..(1048)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1053)..(1056)
<223>  n is a, c, g, or t

<400>  134
taaaaggcgt tgaaacagaa tgagccagac agcaaggaca aggtggccaa cagcaaggag       60

tccaaaaagc cctctattga cgagatccac gatgttattg ctcatgaggt ttccgagctc      120

gatgctggga agaagaagtg atttgtatat aagaaataaa tgagatatag taaaggagtg      180

caagagaatg gcaaggtggt caaattctat attacttgca gtcactggtt cctcgttgac      240

atgaatgaag ttaccgttgg catagctgat ttaatatata actgtccaac taactctcac      300

ctagatataa cccatgtgtg tgtttccaat catcaatgcg gccgcgccta cttaagcaac      360

gggcttgata acagcggggg gggtgcccac gttgttgcgg ttgcggaaga acagaacacc      420

cttaccagca ccctcggcac cagcgctggg ctcaacccac tggcacatac gcgcactgcg      480

gtacatggcg cggatgaagc cacgaggacc atcctggaca tcagcccggt agtgcttgcc      540

catgatgggc ttaatggcct cggtggcctc gtccgcgttg tagaagggga tgctgctgac      600

gtagtggtgg aggacatgag tctcgatgat gccgtggaga aggtggcggc cgatgaagcc      660

catctcacgg tcaatggtag cagcggcacc acggacgaag ttccactcgt cgttggtgta      720

gtggggaagg gtagggtcgg tgtgctggag gaaggtgatg gcaacgagcc agtggttaac      780

ccagaggtag ggaacaaagt accagatggc catgttgtag aaaccgaact tctgaacgag      840

gaagtacaga gcagtggcca tcagaccgat accaatatcg ctgaggacga tgagcttagc      900

gtcactgttc tcgtacagag ggctgcgggg atcgaagtgg ttaacaccac cgccgaggcc      960

gttatgcttg cccttgccgc gaccctcacg ctggcgctcg tggtagttgt ggccggtaac     1020

attggtgatg aggtagttgg gccagccnac gannnnctca gtaagatgag cgagctcgtg     1080

ggtcatcttt ccgagacgag tagcctgctg ctcgcgggtt cggggaacga agaccatgtc     1140

acgctccatg ttgccagtgg ccttgtggtg ctttcggtgg gagatttgcc agctgaagta     1200

ggggacaagg agggaagagt gaagaaccca gccagtaatg tcgttgatga tgcgagaatc     1260

ggagaaagca ccgtgaccgc actcatgggc aataacccag agaccagtac cgaaaagacc     1320

ctgaagaacg gtgtacacgg cccacagacc agcgcgggcg ggggtggagg ggatatattc     1380

gggggtcaca aagttgtacc agatgctgaa agtggtagtc aggaggacaa tgtcgcggag     1440

gatataaccg tatcccttga gagcggagcg cttgaagcag tgcttaggga tggcattgta     1500

gatgtccttg atggtaaagt cgggaacctc gaactggttg ccgtaggtgt cgagcatgac     1560

accatactcg gacttgggct tggcgatatc aacctcggac atggacgaga gcgatgtgga     1620

agaggccgag tggcggggag agtctgaagg agagacggcg gcagactcag aatccgtcac     1680

agtagttgag gtgacggtgc gtctaagcgc agggttctgc ttgggcagag ccgaagtgga     1740

cgccatgggc aggacctgtg ttagtacatt gtcggggagt catcaattgg ttcgacaggt     1800

tgtcgactgt tagtatgagc tcaattgggc tctggtgggt cgatgacact tgtcatctgt     1860

ttctgttggg tcatgtttcc atcaccttct atggtactca caattcgtcc gattcgcccg     1920

aatccgttaa taccgacttt gatggccatg ttgatgtgtg tttaattcaa gaatgaatat     1980

agagaagaga agaagaaaaa agattcaatt gagccggcga tgcagaccct tatataaatg     2040

ttgccttgga cagacggagc aagcccgccc aaacctacgt tcggtataat atgttaagct     2100

ttttaacaca aaggtttggc ttggggtaac ctgatgtggt gcaaaagacc gggcgttggc     2160

gagccattgc gcgggcgaat ggggccgtga ctcgtctcaa attcgagggc gtgcctcaat     2220

tcgtgccccc gtggcttttt cccgccgttt ccgccccgtt tgcaccactg cagccgcttc     2280

tttggttcgg acaccttgct gcgagctagg tgccttgtgc tacttaaaaa gtggcctccc     2340

aacaccaaca tgacatgagt gcgtgggcca agacacgttg gcggggtcgc agtcggctca     2400

atggcccgga aaaaacgctg ctggagctgg ttcggacgca gtccgccgcg gcgtatggat     2460

atccgcaagg ttccatagcg ccattgccct ccgtcggcgt ctatcccgca acctctaaat     2520

agagcgggaa tataacccaa gcttcttttt tttcctttaa cacgcacacc cccaactatc     2580

atgttgctgc tgctgtttga ctctactctg tggaggggtg ctcccaccca acccaaccta     2640

caggtggatc cggcgctgtg attggctgat aagtctccta tccggactaa ttctgaccaa     2700

tgggacatgc gcgcaggacc caaatgccgc aattacgtaa ccccaacgaa atgcctaccc     2760

ctctttggag cccagcggcc ccaaatcccc ccaagcagcc cggttctacc ggcttccatc     2820

tccaagcaca agcagcccgg aattctctct cttgagcttt tccataacaa gttcttctgc     2880

ctccaggaag tccatgggtg gtttgatcat ggttttggtg tagtggtagt gcagtggtgg     2940

tattgtgact ggggatgtag ttgagaataa gtcatacaca agtcagcttt cttcgagcct     3000

catataagta taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa     3060

cacaacaaca tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca     3120

tactcgatca gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca     3180

cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt     3240

ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt     3300

ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc     3360

aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt     3420

cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac     3480

aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag     3540

agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg     3600

agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt     3660

ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg     3720

tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg     3780

cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt     3840

aagagcaagt tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc     3900

gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc     3960

cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt     4020

gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat     4080

tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt     4140

atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata     4200

gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc     4260

gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt     4320

gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg     4380

tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga     4440

cgagtcagac agatactcgt cgaccttttc cttgggaacc accaccgtca gcccttctga     4500

ctcacgtatt gtagccaccg acacaggcaa cagtccgtgg atagcagaat atgtcttgtc     4560

ggtccatttc tcaccaactt taggcgtcaa gtgaatgttg cagaagaagt atgtgccttc     4620

attgagaatc ggtgttgctg atttcaataa agtcttgaga tcagtttggc cagtcatgtt     4680

gtggggggta attggattga gttatcgcct acagtctgta caggtatact cgctgcccac     4740

tttatacttt ttgattccgc tgcacttgaa gcaatgtcgt ttaccaaaag tgagaatgct     4800

ccacagaaca caccccaggg tatggttgag caaaaaataa acactccgat acggggaatc     4860

gaaccccggt ctccacggtt ctcaagaagt attcttgatg agagcgtatc gatcgaggaa     4920

gaggacaagc ggctgcttct taagtttgtg acatcagtat ccaaggcacc attgcaagga     4980

ttcaaggctt tgaacccgtc atttgccatt cgtaacgctg gtagacaggt tgatcggttc     5040

cctacggcct ccacctgtgt caatcttctc aagctgcctg actatcagga cattgatcaa     5100

cttcggaaga aacttttgta tgccattcga tcacatgctg gtttcgattt gtcttagagg     5160

aacgcatata cagtaatcat agagaataaa cgatattcat ttattaaagt agatagttga     5220

ggtagaagtt gtaaagagtg ataaatagcg gccgcttagg ccttgtctgc cttggcaacc     5280

attcgagcga aggtgcccag gtaggagata agaatgccga caccttcgag catgggagga     5340

gatcggtagg gcaggttgtg cttcttgcac agctgctcca ctttgatgga ggcagcggtg     5400

aggttgtgtc tgggaagagt aggccacagg tgatgttcga tctggtagtt gaggccaccg     5460

aaaaaccagt ctgtgacgag gcctcgctga acgttcatgg tctcgtgaat ctgtccagca     5520

cagaagccat gaccatccca cacggagtcc tgaatcttct ccagagggta gtggttcatg     5580

aagacaacga tggcaattcc gaagccacca agcagctcgg agacaaagaa caccatgagt     5640

ccggtgagaa acgagggcat gtagaaatag tagaacaagg ccttgagacc ccagtggagt     5700

gccagaccga cggactcctt ctcgtactgt cttcgatagt actgattgct tcgatccttg     5760

agacccgtgg cagtatgaat ggactgaaag caccagatga atcgcagaag ggcacagatg     5820

aaaaagaagt aatactgttg gtacttgatc atccgtcgag aaaacgggcc agctcgctcg     5880

acgtcctcct tggaccacgc caggagaggc aggttgtcga tgtcgggatc gtgaccctgc     5940

acgttggtgg cggagtgatg agcgttgtgt cggtccttcc accaagtgac agaaaagccc     6000

tgaagcacgt ttccgaagag caggccaatg gcattgttga tggatcggtc cttgaacagc     6060

tggtgatggc aaatgtcgtg cgagagccat cccatctgtt gaaagtgaat gccgaggacg     6120

atagcaccaa ccaggtacca gtgccactgg gtcatcaaca gcacggcaag gacacccagt     6180

ccgagagtcg acagggtctt gtaagcgtac cacatgggag aggcatcgaa catgcctgca     6240

gcaatgagct cgtctcgcag ctttcggaaa tcctcctgag gttcgtcgga gtttggcttg     6300

ggaggtgttg gagtcagggg agaggaaggt tccatgatgg gcattcgtct gagtttggac     6360

acggcgttct cggagtgcat aaccatgaag acgtcggtag catcccgacc tcggtagttc     6420

tcgataatgt cggcacctcc gggatggtgg ttgagccagg cagaaacgtc gtaggtgaca     6480

ccgtccacgg tgagaggcag agcgggtcgc ttgaccatgg atcccatgac ggccagagag     6540

tcgtagtagg tagcgggagg aagtccggca ggtcgagcgg gaccggcgcc ctcctttttg     6600

cctcggttct ttcgaggctt ccgaatgtag gtctggacgt agaagttgag gaacagaagc     6660

agcaccgtgc cgacatacca gtagttgaag atccaggcaa acattcgcat accgtcctgc     6720

cggtagcagg gcacgtttcg gtacttccag acgatgtaga atccgacgtt gaactggata     6780

atttgcatgg aggtaatgag gttcttgggc atagggaagt tcagcttgat gagtcgcgtc     6840

caatagtaac cgtacatgat ccagtgaatg aagccgttga gcagaacaaa gatccagacg     6900

ccttcgtttc gatacttgta gaacagccac atgtcgatgg gagctccgag atggtgaaag     6960

aactgcagaa acgacaaggg cttgtccatg aggggaaggt agaaggagtc gatgtactcg     7020

acgaacttgc tgaggtagaa cagctgagtg gtgattcgga acacgtcatt gttgaaggca     7080

gtctcacaat cgccggaaag aataccagtg acagagagag cgtaggccat cgccaggaag     7140

gagccgaacg agtagatgga catgagaaag ttgtatgcgg tgaacagctt tttgagggtg     7200

gcttgtcgct tgaggtccag aggtcgaagc atgaacttga tggccacgaa agcgaccgag     7260

aggtacagaa cctcgcaaga ggaggcgtcc tgccagagct gggcgtagtc caccttggga     7320

agttcctcct ggacgatgga gaccagctcc ttggcagcct ccatggtacc agagctgggt     7380

tagtttgtgt agagagtgtg tgttgctagc gactttcgga ttgtgtcatt acacaaaacg     7440

cgtcgtctcg acactgatct tgtcgtggat actcacggct cggaattctg tgatgtgtag     7500

tttagatttc gaatctgtgg ggaaagaaag gaaaaaagag actggcaacc gattgggaga     7560

gccactgttt atatataccc tagacaagcc ccccgcttgt aagatgttgg tcaatgtaaa     7620

ccagtattaa ggttggcaag tgcaggagaa gcaaggtgtg ggtatcgagc aatggaaatg     7680

tgcggaaggc aaaaaaatga ggccacggcc tattgtcggg gctatatcca gggggcgatt     7740

gaagtacact aacatgacat gtgtccacag accctcaatc tggcctgatg agccaaatcc     7800

atacgcgctt tcgcagctct aaaggctata acaagtcaca ccaccctgct cgacctcagc     7860

gccctcactt tttgttaaga caaactgtac acgctgttcc agcgttttct gcctgcacct     7920

ggtgggacat ttggtgcaac ctaaagtgct cggaacctct gtggtgtcca gatcagcgca     7980

gcagttccga ggtagttttg aggcccttag atgatgcaat ggtgtcagtc gctggatcac     8040

gagtcttaat ggcagtattc gttcttattt gtgccattga gccccgttat cctcgtatct     8100

tctacccccc atcccatccc tttgttggtg caaccctacc catttattgt tgggtgcagc     8160

ccaaccgacg tggagagctt ggcttggcca tataaaaagg ccccccccta gtggcaatgg     8220

cagaaagtca gctgtgagtt gttgaatttg tcatctaggc ggcctggccg tcttctccgg     8280

ggcaatttaa atgttcctct atagtagatc tgcgtacact gtttaaacag agtgtgaaag     8340

actcactatg gtccgggctt atctcgacca atagccaaag tctggagttt ctgagagaaa     8400

aaggcaagat acgtatgtaa caaagcgacg catggtacaa taataccgga ggcatgtatc     8460

atagagagtt agtggttcga tgatggcact ggtgcctggt atgactttat acggctgact     8520

acatatttgt cctcagacat acaattacag tcaagcactt acccttggac atctgtaggt     8580

accccccggc caagacgatc tcagcgtgtc gtatgtcgga ttggcgtagc tccctcgctc     8640

gtcaattggc tcccatctac tttcttctgc ttggctacac ccagcatgtc tgctatggct     8700

cgttttcgtg ccttatctat cctcccagta ttaccaactc taaatgacat gatgtgattg     8760

ggtctacact ttcatatcag agataaggag tagcacagtt gcataaaaag cccaactcta     8820

atcagcttct tcctttcttg taattagtac aaaggtgatt agcgaaatct ggaagcttag     8880

ttggccctaa aaaaatcaaa aaaagcaaaa aacgaaaaac gaaaaaccac agttttgaga     8940

acagggaggt aacgaaggat cgtatatata tatatatata tatataccca cggatcccga     9000

gaccggcctt tgattcttcc ctacaaccaa ccattctcac caccctaatt cacaaccatg     9060

gagtctggac ccatgcctgc tggcattccc ttccctgagt actatgactt ctttatggac     9120

tggaagactc ccctggccat cgctgccacc tacactgctg ccgtcggtct cttcaacccc     9180

aaggttggca aggtctcccg agtggttgcc aagtcggcta acgcaaagcc tgccgagcga     9240

acccagtccg gagctgccat gactgccttc gtctttgtgc acaacctcat tctgtgtgtc     9300

tactctggca tcaccttcta ctacatgttt cctgctatgg tcaagaactt ccgaacccac     9360

acactgcacg aagcctactg cgacacggat cagtccctct ggaacaacgc acttggctac     9420

tggggttacc tcttctacct gtccaagttc tacgaggtca ttgacaccat catcatcatc     9480

ctgaagggac gacggtcctc gctgcttcag acctaccacc atgctggagc catgattacc     9540

atgtggtctg gcatcaacta ccaagccact cccatttgga tctttgtggt cttcaactcc     9600

ttcattcaca ccatcatgta ctgttactat gccttcacct ctatcggatt ccatcctcct     9660

ggcaaaaagt acctgacttc gatgcagatt actcagtttc tggtcggtat caccattgcc     9720

gtgtcctacc tcttcgttcc tggctgcatc cgaacacccg gtgctcagat ggctgtctgg     9780

atcaacgtcg gctacctgtt tcccttgacc tatctgttcg tggactttgc caagcgaacc     9840

tactccaagc gatctgccat tgccgctcag aaaaaggctc agtaagcggc cgcatgagaa     9900

gataaatata taaatacatt gagatattaa atgcgctaga ttagagagcc tcatactgct     9960

cggagagaag ccaagacgag tactcaaagg ggattacacc atccatatcc acagacacaa    10020

gctggggaaa ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg    10080

gggcagccag gatttcaggc acttcggtgt ctcggggtga aatggcgttc ttggcctcca    10140

tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga tgaagaatga    10200

acttgaagtg aaggaattta aatgtaacga aactgaaatt tgaccagata ttgtgtccgc    10260

ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat    10320

ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aagcttccac acaacgtacg    10380

tagggatcag gtgcttagga agctcgacca accacggaga ctgttgaaac tggatgtcgg    10440

taacagcatc tggaatgctg aatgttcctc gaataacaac atatttctcc ttgttgaggt    10500

gatcataagc tatgtatccg gtgattgaag tggaatagaa gtctcctccg aagactgagt    10560

ccaacgtcat gttcgggaaa taccgacaac tctctccaca tgtaaaatca gttcgtagag    10620

gagtgactgg cgcattgaca cagtaggcga tgtttgcaat ccgagaaaac ttggccgtaa    10680

agttgtacag ctcctgggag gcttgaactc gagtttttga aagtgtcgct ggtggctcgc    10740

cgaagaggga ggcatagagg tacgcaacca cttgcccgag cgtgaggttc atgatgccaa    10800

tagtgaatgt catttatcac cgtactgcgc agtatttata tagggctcat cggtccatgt    10860

atagatctgt ccacttatga cacccccatg tctcattaat gtgtaaaggt ggagacgggt    10920

ggagtacagg tacagagttg gaggaaatca ggatagtggg gttaagacat gctccgagtc    10980

caaatttcaa ctctccattg tcacaagacc tctggtttca gagttattac agatctaggc    11040

ctgtttcaag gtgaggggac ctcatctgga tcggcacgac gatcgtcacc ttacagagga    11100

cgtctgtcgc agggaaaggt gatgtggcgc gccagctgca ttaatgaatc ggccaacgcg    11160

cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc    11220

gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat    11280

ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca    11340

ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc    11400

atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc    11460

aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg    11520

gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta    11580

ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg    11640

ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac    11700

acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag    11760

gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat    11820

ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat    11880

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc    11940

gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt    12000

ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct    12060

agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt    12120

ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc    12180

gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac    12240

catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat    12300

cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg    12360

cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata    12420

gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta    12480

tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt    12540

gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag    12600

tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa    12660

gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc    12720

gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt    12780

taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc    12840

tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta    12900

ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa    12960

taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca    13020

tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac    13080

aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg tgaaataccg    13140

cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa    13200

aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca    13260

aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga    13320

acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc    13380

agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc    13440

gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc    13500

cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg    13560

caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac    13620

agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc    13680

ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt    13740

aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga    13800

ctcactatag ggcgaattgg gcccgacgtc gcatgccaag gcgtatatta gttggtggga    13860

accagtgtac gaccgggtcc tgtataacca ggttcagtgg catacttgta ggagttgttc    13920

ccgtggtatt tgggcatggc taagacattt cgccgaccaa tgttaagtgc acaatagccg    13980

atgtagtaga tgtaagccag atggttttgg agcaggtcga ttcgagacca cagattgaaa    14040

gtgcctcgat ggcaggcctc gttttctcct ccttcacaga cagacacttt gtcgagtgca    14100

gggtagacgc tttcttggtc aatgtacact tctccaatgg cgtgtgtgta attggaccaa    14160

tctggcagtc caacgaagat atcgttccag tgggtgagcc tgtagtttcg tcgcttgtcg    14220

tttacttcaa ggcctgtgtc attaaaccac agctggttga tgtagtttgc aaactctgag    14280

tttcctactc tcggctggcc aaagttgatc atggtaggat catgtccaag taatttgaag    14340

tgagttgcga aaagaagagc ttgagcagcg cccagcgagt gaccagtaac atacattttg    14400

tagtcagtgt ggttggtgag gaacttttca aactgaggag cagcattgac catagtttcg    14460

ttgaaggcct tggcgaaccc atcatggatc atgcagcctt tgcactcact agttttgatg    14520

ggaataagac gggggtcttc aaccaccaga gcccgctctt tgaggttgtc aagacctttg    14580

ttctccactt ccaagtctgg tcggactgcc catctctgtt aat                      14623


<210>  135
<211>  14619
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZP2-85m98F

<400>  135
cgatggaagc cggtagaacc gggctgcttg tgcttggaga tggaagccgg tagaaccggg       60

ctgcttgggg ggatttgggg ccgctgggct ccaaagaggg gtaggcattt cgttggggtt      120

acgtaattgc ggcatttggg tcctgcgcgc atgtcccatt ggtcagaatt agtccggata      180

ggagacttat cagccaatca cagcgccgga tccacctgta ggttgggttg ggtgggagca      240

cccctccaca gagtagagtc aaacagcagc agcaacatga tagttggggg tgtgcgtgtt      300

aaaggaaaaa aaagaagctt gggttatatt cccgctctat ttagaggttg cgggatagac      360

gccgacggag ggcaatggcg ctatggaacc ttgcggatat ccatacgccg cggcggactg      420

cgtccgaacc agctccagca gcgttttttc cgggccattg agccgactgc gaccccgcca      480

acgtgtcttg gcccacgcac tcatgtcatg ttggtgttgg gaggccactt tttaagtagc      540

acaaggcacc tagctcgcag caaggtgtcc gaaccaaaga agcggctgca gtggtgcaaa      600

cggggcggaa acggcgggaa aaagccacgg gggcacgaat tgaggcacgc cctcgaattt      660

gagacgagtc acggccccat tcgcccgcgc aatggctcgc caacgcccgg tcttttgcac      720

cacatcaggt taccccaagc caaacctttg tgttaaaaag cttaacatat tataccgaac      780

gtaggtttgg gcgggcttgc tccgtctgtc caaggcaaca tttatataag ggtctgcatc      840

gccggctcaa ttgaatcttt tttcttcttc tcttctctat attcattctt gaattaaaca      900

cacatcaacc atggtcaagc gacccgctct gcctctcacc gtggacggtg tcacctacga      960

cgtttctgcc tggctcaacc accatcccgg aggtgccgac attatcgaga actaccgagg     1020

tcgggatgct accgacgtct tcatggttat gcactccgag aacgccgtgt ccaaactcag     1080

acgaatgccc atcatggaac cttcctctcc cctgactcca acacctccca agccaaactc     1140

cgacgaacct caggaggatt tccgaaagct gcgagacgag ctcattgctg caggcatgtt     1200

cgatgcctct cccatgtggt acgcttacaa gaccctgtcg actctcggac tgggtgtcct     1260

tgccgtgctg ttgatgaccc agtggcactg gtacctggtt ggtgctatcg tcctcggcat     1320

tcactttcaa cagatgggat ggctctcgca cgacatttgc catcaccagc tgttcaagga     1380

ccgatccatc aacaatgcca ttggcctgct cttcggaaac gtgcttcagg gcttttctgt     1440

cacttggtgg aaggaccgac acaacgctca tcactccgcc accaacgtgc agggtcacga     1500

tcccgacatc gacaacctgc ctctcctggc gtggtccaag gaggacgtcg agcgagctgg     1560

cccgttttct cgacggatga tcaagtacca acagtattac ttctttttca tctgtgccct     1620

tctgcgattc atctggtgct ttcagtccat tcatactgcc acgggtctca aggatcgaag     1680

caatcagtac tatcgaagac agtacgagaa ggagtccgtc ggtctggcac tccactgggg     1740

tctcaaggcc ttgttctact atttctacat gccctcgttt ctcaccggac tcatggtgtt     1800

ctttgtctcc gagctgcttg gtggcttcgg aattgccatc gttgtcttca tgaaccacta     1860

ccctctggag aagattcagg actccgtgtg ggatggtcat ggcttctgtg ctggacagat     1920

tcacgagacc atgaacgttc agcgaggcct cgtcacagac tggtttttcg gtggcctcaa     1980

ctaccagatc gaacatcacc tgtggcctac tcttcccaga cacaacctca ccgctgcctc     2040

catcaaagtg gagcagctgt gcaagaagca caacctgccc taccgatctc ctcccatgct     2100

cgaaggtgtc ggcattctta tctcctacct gggcaccttc gctcgaatgg ttgccaaggc     2160

agacaaggcc taagcggccg cattgatgat tggaaacaca cacatgggtt atatctaggt     2220

gagagttagt tggacagtta tatattaaat cagctatgcc aacggtaact tcattcatgt     2280

caacgaggaa ccagtgactg caagtaatat agaatttgac caccttgcca ttctcttgca     2340

ctcctttact atatctcatt tatttcttat atacaaatca cttcttcttc ccagcatcga     2400

gctcggaaac ctcatgagca ataacatcgt ggatctcgtc aatagagggc tttttggact     2460

ccttgctgtt ggccaccttg tccttgctgt ttaaacatcg tggttaatgc tgctgtgtgc     2520

tgtgtgtgtg tgttgtttgg cgctcattgt tgcgttatgc agcgtacacc acaatattgg     2580

aagcttatta gcctttctat tttttcgttt gcaaggctta acaacattgc tgtggagagg     2640

gatggggata tggaggccgc tggagggagt cggagaggcg ttttggagcg gcttggcctg     2700

gcgcccagct cgcgaaacgc acctaggacc ctttggcacg ccgaaatgtg ccacttttca     2760

gtctagtaac gccttaccta cgtcattcca tgcgtgcatg tttgcgcctt ttttcccttg     2820

cccttgatcg ccacacagta cagtgcactg tacagtggag gttttggggg ggtcttagat     2880

gggagctaaa agcggcctag cggtacacta gtgggattgt atggagtggc atggagccta     2940

ggtggagcct gacaggacgc acgaccggct agcccgtgac agacgatggg tggctcctgt     3000

tgtccaccgc gtacaaatgt ttgggccaaa gtcttgtcag ccttgcttgc gaacctaatt     3060

cccaattttg tcacttcgca cccccattga tcgagcccta acccctgccc atcaggcaat     3120

ccaattaagc tcgcattgtc tgccttgttt agtttggctc ctgcccgttt cggcgtccac     3180

ttgcacaaac acaaacaagc attatatata aggctcgtct ctccctccca accacactca     3240

cttttttgcc cgtcttccct tgctaacaca aaagtcaaga acacaaacaa ccaccccaac     3300

ccccttacac acaagacata tctacagcaa tggccatggc tctctccctt actaccgagc     3360

agctgctcga gcgacccgac ctggttgcca tcgacggcat tctctacgat ctggaaggtc     3420

ttgccaaggt ccatcccgga tccgacttga tcctcgcttc tggtgcctcc gatgcttctc     3480

ctctgttcta ctccatgcac ccttacgtca agcccgagaa ctcgaagctg cttcaacagt     3540

tcgtgcgagg caagcacgac cgaacctcca aggacattgt ctacacctac gactctccct     3600

ttgcacagga cgtcaagcga actatgcgag aggtcatgaa aggtcggaac tggtatgcca     3660

cacctggatt ctggctgcga accgttggca tcattgctgt caccgccttt tgcgagtggc     3720

actgggctac taccggaatg gtgctgtggg gtctcttgac tggattcatg cacatgcaga     3780

tcggcctgtc cattcagcac gatgcctctc atggtgccat cagcaaaaag ccctgggtca     3840

acgctctctt tgcctacggc atcgacgtca ttggatcgtc cagatggatc tggctgcagt     3900

ctcacatcat gcgacatcac acctacacca atcagcatgg tctcgacctg gatgccgagt     3960

ccgcagaacc attccttgtg ttccacaact accctgctgc caacactgct cgaaagtggt     4020

ttcaccgatt ccaggcctgg tacatgtacc tcgtgcttgg agcctacggc gtttcgctgg     4080

tgtacaaccc tctctacatc ttccgaatgc agcacaacga caccattccc gagtctgtca     4140

cagccatgcg agagaacggc tttctgcgac ggtaccgaac ccttgcattc gttatgcgag     4200

ctttcttcat ctttcgaacc gccttcttgc cctggtatct cactggaacc tccctgctca     4260

tcaccattcc tctggtgccc actgctaccg gtgccttcct caccttcttt ttcatcttgt     4320

ctcacaactt cgatggctcg gagcgaatcc ccgacaagaa ctgcaaggtc aagagctccg     4380

agaaggacgt tgaagccgat cagatcgact ggtacagagc tcaggtggag acctcttcca     4440

cctacggtgg acccattgcc atgttcttta ctggcggtct caacttccag atcgagcatc     4500

acctctttcc tcgaatgtcg tcttggcact atcccttcgt gcagcaagct gtccgagagt     4560

gttgcgaacg acacggagtt cggtacgtct tctaccctac cattgtgggc aacatcattt     4620

ccaccctcaa gtacatgcac aaagtcggtg tggttcactg tgtcaaggac gctcaggatt     4680

cctaagcggc cgcatgagaa gataaatata taaatacatt gagatattaa atgcgctaga     4740

ttagagagcc tcatactgct cggagagaag ccaagacgag tactcaaagg ggattacacc     4800

atccatatcc acagacacaa gctggggaaa ggttctatat acactttccg gaataccgta     4860

gtttccgatg ttatcaatgg gggcagccag gatttcaggc acttcggtgt ctcggggtga     4920

aatggcgttc ttggcctcca tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa     4980

cagaagcaga tgaagaatga acttgaagtg aaggaattta aatgtaacga aactgaaatt     5040

tgaccagata ttgtgtccgc ggtggagctc cagcttttgt tccctttagt gagggttaat     5100

ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac     5160

aagcttccac acaacgtacg ggcgtcgttg cttgtgtgat ttttgaggac ccatcccttt     5220

ggtatataag tatactctgg ggttaaggtt gcccgtgtag tctaggttat agttttcatg     5280

tgaaataccg agagccgagg gagaataaac gggggtattt ggacttgttt ttttcgcgga     5340

aaagcgtcga atcaaccctg cgggccttgc accatgtcca cgacgtgttt ctcgccccaa     5400

ttcgcccctt gcacgtcaaa attaggcctc catctagacc cctccataac atgtgactgt     5460

ggggaaaagt ataagggaaa ccatgcaacc atagacgacg tgaaagacgg ggaggaacca     5520

atggaggcca aagaaatggg gtagcaacag tccaggagac agacaaggag acaaggagag     5580

ggcgcccgaa agatcggaaa aacaaacatg tccaattggg gcagtgacgg aaacgacacg     5640

gacacttcag tacaatggac cgaccatctc caagccaggg ttattccggt atcaccttgg     5700

ccgtaacctc ccgctggtac ctgatattgt acacgttcac attcaatata ctttcagcta     5760

caataagaga ggctgtttgt cgggcatgtg tgtccgtcgt atggggtgat gtccgagggc     5820

gaaattcgct acaagcttaa ctctggcgct tgtccagtat gaatagacaa gtcaagacca     5880

gtggtgccat gattgacagg gaggtacaag acttcgatac tcgagcatta ctcggacttg     5940

tggcgattga acagacgggc gatcgcttct cccccgtatt gccggcgcgc cagctgcatt     6000

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct     6060

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa     6120

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa     6180

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc     6240

tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga     6300

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc     6360

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt     6420

ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct     6480

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg     6540

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta     6600

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct     6660

acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa     6720

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt     6780

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta     6840

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat     6900

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa     6960

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct     7020

cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta     7080

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct     7140

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg     7200

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa     7260

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt     7320

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta     7380

catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca     7440

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta     7500

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct     7560

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg     7620

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac     7680

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact     7740

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa     7800

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt     7860

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat     7920

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg     7980

atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa     8040

gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc     8100

aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga     8160

gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag     8220

ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt     8280

ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta     8340

gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag     8400

cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg     8460

cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga     8520

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     8580

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     8640

cagtgaattg taatacgact cactataggg cgaattgggc ccgacgtcgc atgcgctgat     8700

gacactttgg tctgaaagag atgcattttg aatcccaaac ttgcagtgcc caagtgacat     8760

acatctccgc gttttggaaa atgttcagaa acagttgatt gtgttggaat ggggaatggg     8820

gaatggaaaa atgactcaag tatcaattcc aaaaacttct ctggctggca gtacctactg     8880

tccatactac tgcattttct ccagtcaggc cactctatac tcgacgacac agtagtaaaa     8940

cccagataat ttcgacataa acaagaaaac agacccaata atatttatat atagtcagcc     9000

gtttgtccag ttcagactgt aatagccgaa aaaaaatcca aagtttctat tctaggaaaa     9060

tatattccaa tatttttaat tcttaatctc atttatttta ttctagcgaa atacatttca     9120

gctacttgag acatgtgata cccacaaatc ggattcggac tcggttgttc agaagagcat     9180

atggcattcg tgctcgcttg ttcacgtatt cttcctgttc catctcttgg ccgacaatca     9240

cacaaaaatg gggttttttt tttaattcta atgattcatt acagcaaaat tgagatatag     9300

cagaccacgt attccataat caccaaggaa gttcttgggc gtcttaatta actcacctgc     9360

aggattgaga ctatgaatgg attcccgtgc ccgtattact ctactaattt gatcttggaa     9420

cgcgaaaata cgtttctagg actccaaaga atctcaactc ttgtccttac taaatatact     9480

acccatagtt gatggtttac ttgaacagag aggacatgtt cacttgaccc aaagtttctc     9540

gcatctcttg gatatttgaa caacggcgtc cactgaccgt cagttatcca gtcacaaaac     9600

ccccacattc atacattccc atgtacgttt acaaagttct caattccatc gtgcaaatca     9660

aaatcacatc tattcattca tcatatataa acccatcatg tctactaaca ctcacaactc     9720

catagaaaac atcgactcag aacacacgct ccatgcggcc gcttactgag ccttggcacc     9780

gggctgcttc tcggccattc gagcgaactg ggacaggtat cggagcagga tgacgagacc     9840

ttcatggggc agagggtttc ggtaggggag gttgtgcttc tggcacagct gttccacctg     9900

gtaggaaacg gcagtgaggt tgtgtcgagg cagggtgggc cagagatggt gctcgatctg     9960

gtagttcagg cctccaaaga accagtcagt aatgatgcct cgtcgaatgt tcatggtctc    10020

atggatctga cccacagaga agccatgtcc gtcccagacg gaatcaccga tcttctccag    10080

agggtagtgg ttcatgaaga ccacgatggc aattccgaag ccaccgacga gctcggaaac    10140

aaagaacacc agcatcgagg tcaggatgga gggcataaag aagaggtgga acagggtctt    10200

gagagtccag tgcagagcga gtccaatggc ctctttcttg tactgagatc ggtagaactg    10260

gttgtctcgg tccttgaggg atcgaacggt cagcacagac tggaaacacc agatgaatcg    10320

caggagaata cagatgacca ggaaatagta ctgttggaac tgaatgagct ttcgggagat    10380

gggagaagct cgagtgacat cgtcctcgga ccaggcgagc agaggcaggt tatcaatgtc    10440

gggatcgtga ccctgaacgt tggtagcaga atgatgggcg ttgtgtctgt ccttccacca    10500

ggtcacggag aagccctgga gtccgttgcc aaagaccaga cccaggacgt tattccagtt    10560

tcggttcttg aaggtctggt ggtggcagat gtcatgagac agccatccca tttgctggta    10620

gtgcataccg agcacgagag caccaatgaa gtacaggtgg tactggacca gcatgaagaa    10680

ggcaagcacg ccaagaccca gggtggtcaa gatcttgtac gagtaccaga ggggagaggc    10740

gtcaaacatg ccagtggcga tcagctcttc tcggagcttt cggaaatcct cctgagcttc    10800

gttgacggca gcctggggag gcagctcgga agcctggttg atcttgggca ttcgcttgag    10860

cttgtcgaag gcttcctgag agtgcataac catgaaggcg tcagtagcat ctcgtccctg    10920

gtagttctca atgatttcag ctccaccagg gtggaagttc acccaagcgg agacgtcgta    10980

cacctttccg tcgatgacga ggggcagagc ctgtcgagaa gccttcacgg atcccatgac    11040

ggccagagag tcgtagtagg tagcgggagg aagtccggca ggtcgagcgg gaccggcgcc    11100

ctgaatcttt ttggctccct tgtgctttcg gacgatgtag gtctgcacgt agaagttgag    11160

gaacagacac aggacagtac caacgtagaa gtagttgaaa aaccagccaa acattctcat    11220

tccatcttgt cggtagcagg gaatgttccg gtacttccag acgatgtaga agccaacgtt    11280

gaactgaatg atctgcatag aagtaatcag ggacttgggc atagggaact tgagcttgat    11340

cagtcgggtc caatagtagc cgtacatgat ccagtgaatg aagccgttga gcagcacaaa    11400

gatccaaacg gcttcgtttc ggtagttgta gaacagccac atgtccatag gagctccgag    11460

atggtgaaag aactgcaacc aggtcagagg cttgcccatg aggggcagat agaaggagtc    11520

aatgtactcg aggaacttgc tgaggtagaa cagctgagtg gtgattcgga agacattgtt    11580

gtcgaaagcc ttctcgcagt tgtcggacat gacaccaatg gtgtacatgg cgtaggccat    11640

agagaggaag gagcccagcg agtagatgga catgagcagg ttgtagttgg tgaacacaaa    11700

cttcattcga gactgaccct tgggtccgag aggaccaagg gtgaacttca ggatgacgaa    11760

ggcgatggag aggtacagca cctcgcagtg cgaggcatca gaccagagct gagcatagtc    11820

gaccttggga agaacctcct ggccaatgga gacgatttcg ttcacgacct ccatggttgt    11880

gaattagggt ggtgagaatg gttggttgta gggaagaatc aaaggccggt ctcgggatcc    11940

gtgggtatat atatatatat atatatatac gatccttcgt tacctccctg ttctcaaaac    12000

tgtggttttt cgtttttcgt tttttgcttt ttttgatttt tttagggcca actaagcttc    12060

cagatttcgc taatcacctt tgtactaatt acaagaaagg aagaagctga ttagagttgg    12120

gctttttatg caactgtgct actccttatc tctgatatga aagtgtagac ccaatcacat    12180

catgtcattt agagttggta atactgggag gatagataag gcacgaaaac gagccatagc    12240

agacatgctg ggtgtagcca agcagaagaa agtagatggg agccaattga cgagcgaggg    12300

agctacgcca atccgacata cgacacgctg agatcgtctt ggccgggggg tacctacaga    12360

tgtccaaggg taagtgcttg actgtaattg tatgtctgag gacaaatatg tagtcagccg    12420

tataaagtca taccaggcac cagtgccatc atcgaaccac taactctcta tgatacatgc    12480

ctccggtatt attgtaccat gcgtcgcttt gttacatacg tatcttgcct ttttctctca    12540

gaaactccag aattctctct cttgagcttt tccataacaa gttcttctgc ctccaggaag    12600

tccatgggtg gtttgatcat ggttttggtg tagtggtagt gcagtggtgg tattgtgact    12660

ggggatgtag ttgagaataa gtcatacaca agtcagcttt cttcgagcct catataagta    12720

taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa cacaacaaca    12780

tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca tactcgatca    12840

gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca cgctctctat    12900

atacacagtt aaattacata tccatagtct aacctctaac agttaatctt ctggtaagcc    12960

tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt ctggccgtac    13020

agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc aacagttcgg    13080

tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt cagaataagc    13140

cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac aaactcgggg    13200

tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag agagcccttg    13260

caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg agaggggact    13320

aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt ctgttcagag    13380

acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg tacaccgtgg    13440

gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg cttgacagtg    13500

ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt aagagcaagt    13560

tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc gatatgggtt    13620

ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc cttggtggtg    13680

gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt gagcactcga    13740

gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat tttggtggtg    13800

aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt atctggggca    13860

gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata gactggacta    13920

tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc gcctttgccg    13980

acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt gttgtcggcc    14040

aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg tatcgtcaaa    14100

gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga cgagtcagac    14160

agatactcgt cgaccttttc cttgggaacc accaccgtca gcccttctga ctcacgtatt    14220

gtagccaccg acacaggcaa cagtccgtgg atagcagaat atgtcttgtc ggtccatttc    14280

tcaccaactt taggcgtcaa gtgaatgttg cagaagaagt atgtgccttc attgagaatc    14340

ggtgttgctg atttcaataa agtcttgaga tcagtttggc cagtcatgtt gtggggggta    14400

attggattga gttatcgcct acagtctgta caggtatact cgctgcccac tttatacttt    14460

ttgattccgc tgcacttgaa gcaatgtcgt ttaccaaaag tgagaatgct ccacagaaca    14520

caccccaggg tatggttgag caaaaaataa acactccgat acggggaatc gaaccccggt    14580

ctccacggtt ctcaagaagt attcttgatg agagcgtat                           14619


<210>  136
<211>  15262
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZK16-ML8N

<400>  136
gtacgcattc ttgacttgct ccccagttgc cctttttggt cataactcag ccccgactct       60

ctccccaaag aggagatgga cttacaatag atacgagaca acagatattt tcggccacct      120

ggtgcaattg gtctgtttta ttacccccaa taatatccac agcgtccgaa tcagctcgac      180

aagctctttc tcagcatact gatcttaccc catacccccc agacagcggt gccatatcca      240

tacccgcact acctgagaca gtaaactcag ggcaaggagc ggtcttggtc catcgcgacg      300

ctgtaagctt tgcctttgcg ttttgtgatc caccacacgg cagtcaaggt ctcggttgaa      360

agatgggtct ggcaatgtgg attgctgaat cattgacata taaatacgag tgatcctgcc      420

atttcccaat ctttagaaat catcttttct tcacacatac aaaaaacaca catcacaatg      480

cagttctccg ccgccgccgt ccttgccctc gccgccttcg tctctgccca ggccgcctcc      540

gctgcttctg aagctggtgg tgccgttgtc tccggtgcct ccggtgccgc tgccgccgct      600

tcttctgctg tcattggctc cgagtctcag gccgcttctt ctgctgcctc ttctgctgct      660

gagtctgctt cttccgctgc ctctgagtcc gcctcctccg cttccgcctc cgagtccaag      720

gcctctgagt ccgcttccaa gtccgcctcc aaggcttctg agtctgcttc caaggccgag      780

tcttctgctg ccaaggcttc ctccgctgct ggttctgctt cttccgccgc tgcctctgcc      840

accaagtctg gtgatgcctc cggctcttcc aagccttccg gctctgcctc ctctaccggt      900

gacaagtccg gtgctggtat tgttgccccc gctgttggtg ccatcgttgt cggtgctctt      960

gctcagctca tctaagcggt ctttgtacaa ctacccactt aatatttata ttatgatata     1020

gactgcaata ctattgcatg tgtaatgctt cgtttgaaag tcgtagtccg gccattcatg     1080

gcagtgtatg gcagtattgc aaccaattgg cttaataaca tatacaaagt ggcaattctg     1140

tgttcgaacc atcacacaac ccaacatatt tgacattgct acaagtattc gacttgttct     1200

tcggccgcac gacatgcatt gctagcagca cacttgcaga agcttggcca atggttaggt     1260

tgacctttaa ccagtaaaac gcaatatagg ttgccaatta acaaacaaca cagcccagaa     1320

ctggtgtaaa gggcttgggt tttgtgcaat caactcattg actcacctcc cccaatgctc     1380

catgaaccca tgaatccata gtacctccat cccaattcag agaggctcag aaacacgcaa     1440

ttggcaaaag taaacaaagg tctcgaaacc ttaaggggtg cagtgttgca aagttaaaat     1500

tacactgttg gatggtgttc cactcaaact gtggatggat cagacggttg cactaaacca     1560

agattcaata tgctctacaa gtagcgtgcc atgaggtgta taatatcaca catgatcaga     1620

gttaaggcct ggaaaaaaag tgtccgcggc tatgtaacac tgctgaatac agtgtgtgcc     1680

gtttccacgg actggaatga atgcactcaa cacgttggta gctaacttcc ccaactattg     1740

acttcacttt actgtagcta ctctaagtac gataatgaca tcatgaatca ccaactttat     1800

cttccccacc gaaagtaaga gatgaaagag aggctaacag cttttgtaag aaaatcacaa     1860

aatctactta ccgtattaca aaagtacaag tatcagtaac caggcgcgcc agctgcatta     1920

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc     1980

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     2040

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     2100

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     2160

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     2220

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     2280

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     2340

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     2400

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     2460

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     2520

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     2580

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     2640

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     2700

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     2760

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     2820

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     2880

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     2940

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac     3000

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc     3060

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg     3120

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag     3180

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc     3240

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac     3300

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag     3360

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac     3420

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg     3480

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc     3540

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact     3600

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg     3660

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa     3720

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt     3780

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg     3840

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga     3900

tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag     3960

cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca     4020

ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag     4080

tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg     4140

gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt     4200

tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag     4260

agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc     4320

gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc     4380

gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa     4440

gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca     4500

aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc     4560

agtgaattgt aatacgactc actatagggc gaattgggcc cgacgtcgca tgctatgtac     4620

actgtgtgag tgtagagggc aatggtggtg acaactgagc tactatacac acacagcttg     4680

tactggtata agttgtgagc tacagtacaa gtaatactac aaaattcagt atgtacaagt     4740

atgccggtac ggcagtagca tgtatcaaag agagacagaa agaaggctgg aaagcgaagg     4800

ccagctgagc gtgggaaccg cagtacgtgg aagtgccaaa cctgcgacag aatgacatgg     4860

tcttgcactt tttaccggta ccggtacagt acgataccgg tacgcacttg ctacagtaga     4920

tcatagacca ccagaataac cctgcattag cgccacgttg ccaagccctc aactttcttt     4980

gcagctacac cttttacacc ttttaggctg ccaggaagag cataaatgaa ttgtacttgg     5040

gggcacagaa tttgggttga agtgttcctg ggagagaatg tgcttggctg tatagttggg     5100

tggctctcct tcatagtaga tgtgaggtta cagcacttgt agattgtgta cgcgttctgt     5160

ttaaggtggt accagtatta tgggttgacg tgaaagaaag ggaggaggca ggggtacagc     5220

aagagtgtgg gcgcacactc acacatgaag caagacctgt ttcgtcattt ctatttgttt     5280

aacaaggacg acataatata cttaggacag catgggccga ccggcaggaa taatctgcaa     5340

acacaatcgt attgtggcgt cccctctcga tcacgatagt tgctaagttt agcagtctga     5400

gagtttggct gtgacgcaag accgagagag agacagagag ttgtcccagt ttgagtactc     5460

gtaccgtact acagagtaaa tcgaaatcga gcgctcactt tctgcacttt gtgaaggcgc     5520

agcattcaac atttgtgtcc agcccctttc cgtttgaagc gacttgacca cgaaatgttg     5580

gaataaagct gtgttacata cgagacctac aagtagagtg tcggatatgc caactgaaca     5640

ggttagatga aagttactca acaacaggag agcggacacc aacgtgcgaa aatgtacgga     5700

tgaacccatt gtctgtccgc atagcaatct gttttcgcca gcagtgcttg tgtcgttgga     5760

ttgctgccat tgtactattt gtactttgtt ccattaacac tcgcatcttg ctcaccgatt     5820

agcagcactc ctgttgcctc actgggcttg tctgtttcag ggtggaatgc atgagcaatg     5880

cgatcgtcaa ggggaggttg aaagcgcgta tggaataact gtggttctat agctgagggg     5940

aggatatcga ggacagccac ggttcagtgg gggatttcgg gagctactca gtaacatgta     6000

ctcttgtccg aacgccctgc tgatggcaaa tatgacaaaa cgcaacaatg tggagcaaca     6060

tttccatttc gagagatatt acatgggttg cattggggcc tgggcatccc gtgcaataac     6120

cgagaccatg tgaaggtgca cgatcgacac ttatccagcc agtcatacgt ggtgtcgcaa     6180

gctaagcaac aatgggattc acgaatctat gtgggggaac gtgcacaggg gaaatgaaac     6240

aaaacggagg agggggtctt tgaaaatgta gagaaatata acaaaaaatc acaaaaacgc     6300

accaaaaata tgccaaaatc gtcaacagtg gaaatcacgc gcccctggct cgacttccca     6360

ctgacatacc ccccctcaac caatatcata tccaccctag ccaatcgctt aattaagttg     6420

cgacacatgt cttgatagta tcttgaattc tctctcttga gcttttccat aacaagttct     6480

tctgcctcca ggaagtccat gggtggtttg atcatggttt tggtgtagtg gtagtgcagt     6540

ggtggtattg tgactgggga tgtagttgag aataagtcat acacaagtca gctttcttcg     6600

agcctcatat aagtataagt agttcaacgt attagcactg tacccagcat ctccgtatcg     6660

agaaacacaa caacatgccc cattggacag atcatgcgga tacacaggtt gtgcagtatc     6720

atacatactc gatcagacag gtcgtctgac catcatacaa gctgaacaag cgctccatac     6780

ttgcacgctc tctatataca cagttaaatt acatatccat agtctaacct ctaacagtta     6840

atcttctggt aagcctccca gccagccttc tggtatcgct tggcctcctc aataggatct     6900

cggttctggc cgtacagacc tcggccgaca attatgatat ccgttccggt agacatgaca     6960

tcctcaacag ttcggtactg ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg     7020

ggggtcagaa taagccagtc ctcagagtcg cccttaggtc ggttctgggc aatgaagcca     7080

accacaaact cggggtcgga tcgggcaagc tcaatggtct gcttggagta ctcgccagtg     7140

gccagagagc ccttgcaaga cagctcggcc agcatgagca gacctctggc cagcttctcg     7200

ttgggagagg ggactaggaa ctccttgtac tgggagttct cgtagtcaga gacgtcctcc     7260

ttcttctgtt cagagacagt ttcctcggca ccagctcgca ggccagcaat gattccggtt     7320

ccgggtacac cgtgggcgtt ggtgatatcg gaccactcgg cgattcggtg acaccggtac     7380

tggtgcttga cagtgttgcc aatatctgcg aactttctgt cctcgaacag gaagaaaccg     7440

tgcttaagag caagttcctt gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg     7500

atgtcgatat gggttttgat catgcacaca taaggtccga ccttatcggc aagctcaatg     7560

agctccttgg tggtggtaac atccagagaa gcacacaggt tggttttctt ggctgccacg     7620

agcttgagca ctcgagcggc aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag     7680

ggcattttgg tggtgaagag gagactgaaa taaatttagt ctgcagaact ttttatcgga     7740

accttatctg gggcagtgaa gtatatgtta tggtaatagt tacgagttag ttgaacttat     7800

agatagactg gactatacgg ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg     7860

gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc cagcaatgac gttgcagctg     7920

atattgttgt cggccaaccg cgccgaaaac gcagctgtca gacccacagc ctccaacgaa     7980

gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg agtcgtactc caaaggcggc     8040

aatgacgagt cagacagata ctcgtcgacc ttttccttgg gaaccaccac cgtcagccct     8100

tctgactcac gtattgtagc caccgacaca ggcaacagtc cgtggatagc agaatatgtc     8160

ttgtcggtcc atttctcacc aactttaggc gtcaagtgaa tgttgcagaa gaagtatgtg     8220

ccttcattga gaatcggtgt tgctgatttc aataaagtct tgagatcagt ttggccagtc     8280

atgttgtggg gggtaattgg attgagttat cgcctacagt ctgtacaggt atactcgctg     8340

cccactttat actttttgat tccgctgcac ttgaagcaat gtcgtttacc aaaagtgaga     8400

atgctccaca gaacacaccc cagggtatgg ttgagcaaaa aataaacact ccgatacggg     8460

gaatcgaacc ccggtctcca cggttctcaa gaagtattct tgatgagagc gtatcgatag     8520

ttggagcaag ggagaaatgt agagtgtgaa agactcacta tggtccgggc ttatctcgac     8580

caatagccaa agtctggagt ttctgagaga aaaaggcaag atacgtatgt aacaaagcga     8640

cgcatggtac aataataccg gaggcatgta tcatagagag ttagtggttc gatgatggca     8700

ctggtgcctg gtatgacttt atacggctga ctacatattt gtcctcagac atacaattac     8760

agtcaagcac ttacccttgg acatctgtag gtaccccccg gccaagacga tctcagcgtg     8820

tcgtatgtcg gattggcgta gctccctcgc tcgtcaattg gctcccatct actttcttct     8880

gcttggctac acccagcatg tctgctatgg ctcgttttcg tgccttatct atcctcccag     8940

tattaccaac tctaaatgac atgatgtgat tgggtctaca ctttcatatc agagataagg     9000

agtagcacag ttgcataaaa agcccaactc taatcagctt cttcctttct tgtaattagt     9060

acaaaggtga ttagcgaaat ctggaagctt agttggccct aaaaaaatca aaaaaagcaa     9120

aaaacgaaaa acgaaaaacc acagttttga gaacagggag gtaacgaagg atcgtatata     9180

tatatatata tatatatacc cacggatccc gagaccggcc tttgattctt ccctacaacc     9240

aaccattctc accaccctaa ttcacaacca tgtctattgg ttcgtccaac cccgtgctct     9300

tggctgcgat tcccttcgtc tacctgtttg tcctcccacg agtcctggct ttcctgcctc     9360

agaaggctca gttcctggcc aaatgtattg tggtcctgat tgccacgctt atcatgtccg     9420

ttgcaggctg cttcatctcg atcgtgtgcg ctcttctgga caagagatac gtcatcaatt     9480

acgttgtgtc gcgattgttc tccttccttg ccgctcgacc gtgtggtgtg acctataaga     9540

ttgttggtga ggaacacctc gataagtacc ctgctatcgt ggtctgtaac catcaatcct     9600

ctatggatat gatggttttg ggacgagttt ttccaaagca ctgcgttgtc atggcgaaga     9660

aggaactcct gtactttccc tttttgggaa tgtttatgaa actgagcaac gctatcttca     9720

tcgaccggaa gaaccacaag aaagccatcg agtctaccac ccaagccgtg gcggacatga     9780

agaagcacaa ctctggaatc tggattttcc cagagggcac ccggtctaga ctggacaagg     9840

cagacctgct gcccttcaag aaaggtgcct ttcatcttgc aattcaggcc cagctcccta     9900

ttctccccat tatctcgcag ggctattccc atatctacga ctcttcgaag cggtacttcc     9960

ccggtggaga gctcgagatc agagtcctgg agcccattcc tacaactggc ctcactactg    10020

atgatgtgaa cgacctgatg gacaagacac gaaaccttat gctcaagcac ttgaaggaga    10080

tggattccca gtattcgtcg agcactgctg aaaatggatc cacgcacatc gacgccgata    10140

ttgccaagtc tacagccacc agcattggca acactgacga cgcaattaca aaacgtcgta    10200

cccctaagga ataagcggcc gcattgatga ttggaaacac acacatgggt tatatctagg    10260

tgagagttag ttggacagtt atatattaaa tcagctatgc caacggtaac ttcattcatg    10320

tcaacgagga accagtgact gcaagtaata tagaatttga ccaccttgcc attctcttgc    10380

actcctttac tatatctcat ttatttctta tatacaaatc acttcttctt cccagcatcg    10440

agctcggaaa cctcatgagc aataacatcg tggatctcgt caatagaggg ctttttggac    10500

tccttgctgt tggccacctt gtccttgctg tttaaaccat catctaaggg cctcaaaact    10560

acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac    10620

caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac    10680

aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg    10740

cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat    10800

gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt    10860

tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca    10920

accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata    10980

tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga    11040

ttcgaaatct aaactacaca tcacaccatg gtctccaacc acctgttcga cgccatgcga    11100

gctgccgctc ccggagacgc acctttcatt cgaatcgaca acgctcggac ctggacttac    11160

gatgacgcca ttgctctttc cggtcgaatc gctggagcta tggacgcact cggcattcga    11220

cccggagaca gagttgccgt gcaggtcgag aagtctgccg aggcgttgat tctctacctg    11280

gcctgtcttc gaaccggagc tgtctacctg cctctcaaca ctgcctacac cctggccgag    11340

ctcgactact tcatcggcga tgccgaaccg cgtctggtgg tcgttgctcc cgcagctcga    11400

ggtggcgtgg agacaattgc caagcgacac ggtgctatcg tcgaaaccct cgacgccgat    11460

ggacgaggct ccttgctgga ccttgctaga gatgagcctg ccgactttgt cgatgcttcg    11520

cgatctgccg acgatctggc tgctattctc tacacttccg gtacaaccgg acgatcgaag    11580

ggtgccatgc ttactcatgg caatctgctc tccaacgctc tcaccttgcg agactattgg    11640

agagttaccg cagacgatcg actcatccat gccttgccaa tctttcacac tcatggtctg    11700

ttcgttgcta cgaacgtcac actgcttgca ggagcctcga tgtttctgct ctccaagttc    11760

gatgccgacg aggtcgtttc tctcatgcca caggccacca tgcttatggg cgtgcccaca    11820

ttctacgttc gattgctgca gagtcctcga ctcgagaagg gtgctgtggc cagcatcaga    11880

ctgttcattt ctggatcagc tcccttgctt gccgaaaccc acgccgagtt tcatgctcgt    11940

actggtcacg ccattctcga gcgatacggc atgacggaaa ccaacatgaa tacttccaac    12000

ccctacgagg gcaagcgtat tgccggaacc gttggttttc ctctgcccga cgtcactgtg    12060

cgagtcaccg atcccgccac cggtctcgtt cttccacctg aagagactgg catgatcgag    12120

atcaagggac ccaacgtctt caagggctat tggcgaatgc ccgaaaagac cgctgccgag    12180

tttaccgcag acggtttctt tatctctgga gatctcggca agatcgaccg agaaggttac    12240

gttcacattg tgggacgagg caaggacctg gtcatttccg gtggctacaa catctatccc    12300

aaagaggtcg aaggcgagat cgaccagatc gagggtgtgg tcgagtctgc tgtcattggt    12360

gttcctcatc ccgatttcgg agaaggtgtc accgctgttg tcgtgtgcaa acctggtgcc    12420

gttctcgacg aaaagaccat cgtgtctgct ctgcaggacc gtcttgcccg atacaagcaa    12480

cccaagcgga ttatctttgc cgacgatctg cctcgaaaca ctatgggaaa ggttcagaag    12540

aacattcttc gacagcaata cgccgatctc tacaccagac gataagcggc cgcatgagaa    12600

gataaatata taaatacatt gagatattaa atgcgctaga ttagagagcc tcatactgct    12660

cggagagaag ccaagacgag tactcaaagg ggattacacc atccatatcc acagacacaa    12720

gctggggaaa ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg    12780

gggcagccag gatttcaggc acttcggtgt ctcggggtga aatggcgttc ttggcctcca    12840

tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga tgaagaatga    12900

acttgaagtg aaggaattta aatagttgga gcaagggaga aatgtagagt gtgaaagact    12960

cactatggtc cgggcttatc tcgaccaata gccaaagtct ggagtttctg agagaaaaag    13020

gcaagatacg tatgtaacaa agcgacgcat ggtacaataa taccggaggc atgtatcata    13080

gagagttagt ggttcgatga tggcactggt gcctggtatg actttatacg gctgactaca    13140

tatttgtcct cagacataca attacagtca agcacttacc cttggacatc tgtaggtacc    13200

ccccggccaa gacgatctca gcgtgtcgta tgtcggattg gcgtagctcc ctcgctcgtc    13260

aattggctcc catctacttt cttctgcttg gctacaccca gcatgtctgc tatggctcgt    13320

tttcgtgcct tatctatcct cccagtatta ccaactctaa atgacatgat gtgattgggt    13380

ctacactttc atatcagaga taaggagtag cacagttgca taaaaagccc aactctaatc    13440

agcttcttcc tttcttgtaa ttagtacaaa ggtgattagc gaaatctgga agcttagttg    13500

gccctaaaaa aatcaaaaaa agcaaaaaac gaaaaacgaa aaaccacagt tttgagaaca    13560

gggaggtaac gaaggatcgt atatatatat atatatatat atacccacgg atcccgagac    13620

cggcctttga ttcttcccta caaccaacca ttctcaccac cctaattcac aaccatggtg    13680

aaggcttctc gacaggctct gcccctcgtc atcgacggaa aggtgtacga cgtctccgct    13740

tgggtgaact tccaccctgg tggagctgaa atcattgaga actaccaggg acgagatgct    13800

actgacgcct tcatggttat gcactctcag gaagccttcg acaagctcaa gcgaatgccc    13860

aagatcaacc aggcttccga gctgcctccc caggctgccg tcaacgaagc tcaggaggat    13920

ttccgaaagc tccgagaaga gctgatcgcc actggcatgt ttgacgcctc tcccctctgg    13980

tactcgtaca agatcttgac caccctgggt cttggcgtgc ttgccttctt catgctggtc    14040

cagtaccacc tgtacttcat tggtgctctc gtgctcggta tgcactacca gcaaatggga    14100

tggctgtctc atgacatctg ccaccaccag accttcaaga accgaaactg gaataacgtc    14160

ctgggtctgg tctttggcaa cggactccag ggcttctccg tgacctggtg gaaggacaga    14220

cacaacgccc atcattctgc taccaacgtt cagggtcacg atcccgacat tgataacctg    14280

cctctgctcg cctggtccga ggacgatgtc actcgagctt ctcccatctc ccgaaagctc    14340

attcagttcc aacagtacta tttcctggtc atctgtattc tcctgcgatt catctggtgt    14400

ttccagtctg tgctgaccgt tcgatccctc aaggaccgag acaaccagtt ctaccgatct    14460

cagtacaaga aagaggccat tggactcgct ctgcactgga ctctcaagac cctgttccac    14520

ctcttcttta tgccctccat cctgacctcg atgctggtgt tctttgtttc cgagctcgtc    14580

ggtggcttcg gaattgccat cgtggtcttc atgaaccact accctctgga gaagatcggt    14640

gattccgtct gggacggaca tggcttctct gtgggtcaga tccatgagac catgaacatt    14700

cgacgaggca tcattactga ctggttcttt ggaggcctga actaccagat cgagcaccat    14760

ctctggccca ccctgcctcg acacaacctc actgccgttt cctaccaggt ggaacagctg    14820

tgccagaagc acaacctccc ctaccgaaac cctctgcccc atgaaggtct cgtcatcctg    14880

ctccgatacc tgtcccagtt cgctcgaatg gccgagaagc agcccggtgc caaggctcag    14940

taagcggccg caagtgtgga tggggaagtg agtgcccggt tctgtgtgca caattggcaa    15000

tccaagatgg atggattcaa cacagggata tagcgagcta cgtggtggtg cgaggatata    15060

gcaacggata tttatgtttg acacttgaga atgtacgata caagcactgt ccaagtacaa    15120

tactaaacat actgtacata ctcatactcg tacccgggca acggtttcac ttgagtgcag    15180

tggctagtgc tcttactcgt acagtgtgca atactgcgta tcatagtctt tgatgtatat    15240

cgtattcatt catgttagtt gc                                             15262


<210>  137
<211>  13075
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZK16-ML

<400>  137
gtacgcattc ttgacttgct ccccagttgc cctttttggt cataactcag ccccgactct       60

ctccccaaag aggagatgga cttacaatag atacgagaca acagatattt tcggccacct      120

ggtgcaattg gtctgtttta ttacccccaa taatatccac agcgtccgaa tcagctcgac      180

aagctctttc tcagcatact gatcttaccc catacccccc agacagcggt gccatatcca      240

tacccgcact acctgagaca gtaaactcag ggcaaggagc ggtcttggtc catcgcgacg      300

ctgtaagctt tgcctttgcg ttttgtgatc caccacacgg cagtcaaggt ctcggttgaa      360

agatgggtct ggcaatgtgg attgctgaat cattgacata taaatacgag tgatcctgcc      420

atttcccaat ctttagaaat catcttttct tcacacatac aaaaaacaca catcacaatg      480

cagttctccg ccgccgccgt ccttgccctc gccgccttcg tctctgccca ggccgcctcc      540

gctgcttctg aagctggtgg tgccgttgtc tccggtgcct ccggtgccgc tgccgccgct      600

tcttctgctg tcattggctc cgagtctcag gccgcttctt ctgctgcctc ttctgctgct      660

gagtctgctt cttccgctgc ctctgagtcc gcctcctccg cttccgcctc cgagtccaag      720

gcctctgagt ccgcttccaa gtccgcctcc aaggcttctg agtctgcttc caaggccgag      780

tcttctgctg ccaaggcttc ctccgctgct ggttctgctt cttccgccgc tgcctctgcc      840

accaagtctg gtgatgcctc cggctcttcc aagccttccg gctctgcctc ctctaccggt      900

gacaagtccg gtgctggtat tgttgccccc gctgttggtg ccatcgttgt cggtgctctt      960

gctcagctca tctaagcggt ctttgtacaa ctacccactt aatatttata ttatgatata     1020

gactgcaata ctattgcatg tgtaatgctt cgtttgaaag tcgtagtccg gccattcatg     1080

gcagtgtatg gcagtattgc aaccaattgg cttaataaca tatacaaagt ggcaattctg     1140

tgttcgaacc atcacacaac ccaacatatt tgacattgct acaagtattc gacttgttct     1200

tcggccgcac gacatgcatt gctagcagca cacttgcaga agcttggcca atggttaggt     1260

tgacctttaa ccagtaaaac gcaatatagg ttgccaatta acaaacaaca cagcccagaa     1320

ctggtgtaaa gggcttgggt tttgtgcaat caactcattg actcacctcc cccaatgctc     1380

catgaaccca tgaatccata gtacctccat cccaattcag agaggctcag aaacacgcaa     1440

ttggcaaaag taaacaaagg tctcgaaacc ttaaggggtg cagtgttgca aagttaaaat     1500

tacactgttg gatggtgttc cactcaaact gtggatggat cagacggttg cactaaacca     1560

agattcaata tgctctacaa gtagcgtgcc atgaggtgta taatatcaca catgatcaga     1620

gttaaggcct ggaaaaaaag tgtccgcggc tatgtaacac tgctgaatac agtgtgtgcc     1680

gtttccacgg actggaatga atgcactcaa cacgttggta gctaacttcc ccaactattg     1740

acttcacttt actgtagcta ctctaagtac gataatgaca tcatgaatca ccaactttat     1800

cttccccacc gaaagtaaga gatgaaagag aggctaacag cttttgtaag aaaatcacaa     1860

aatctactta ccgtattaca aaagtacaag tatcagtaac caggcgcgcc agctgcatta     1920

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc     1980

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     2040

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     2100

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     2160

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     2220

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     2280

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     2340

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     2400

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     2460

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     2520

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     2580

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     2640

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     2700

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     2760

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     2820

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     2880

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     2940

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac     3000

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc     3060

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg     3120

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag     3180

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc     3240

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac     3300

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag     3360

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac     3420

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg     3480

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc     3540

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact     3600

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg     3660

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa     3720

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt     3780

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg     3840

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga     3900

tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag     3960

cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca     4020

ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag     4080

tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg     4140

gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt     4200

tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag     4260

agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc     4320

gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc     4380

gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa     4440

gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca     4500

aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc     4560

agtgaattgt aatacgactc actatagggc gaattgggcc cgacgtcgca tgctatgtac     4620

actgtgtgag tgtagagggc aatggtggtg acaactgagc tactatacac acacagcttg     4680

tactggtata agttgtgagc tacagtacaa gtaatactac aaaattcagt atgtacaagt     4740

atgccggtac ggcagtagca tgtatcaaag agagacagaa agaaggctgg aaagcgaagg     4800

ccagctgagc gtgggaaccg cagtacgtgg aagtgccaaa cctgcgacag aatgacatgg     4860

tcttgcactt tttaccggta ccggtacagt acgataccgg tacgcacttg ctacagtaga     4920

tcatagacca ccagaataac cctgcattag cgccacgttg ccaagccctc aactttcttt     4980

gcagctacac cttttacacc ttttaggctg ccaggaagag cataaatgaa ttgtacttgg     5040

gggcacagaa tttgggttga agtgttcctg ggagagaatg tgcttggctg tatagttggg     5100

tggctctcct tcatagtaga tgtgaggtta cagcacttgt agattgtgta cgcgttctgt     5160

ttaaggtggt accagtatta tgggttgacg tgaaagaaag ggaggaggca ggggtacagc     5220

aagagtgtgg gcgcacactc acacatgaag caagacctgt ttcgtcattt ctatttgttt     5280

aacaaggacg acataatata cttaggacag catgggccga ccggcaggaa taatctgcaa     5340

acacaatcgt attgtggcgt cccctctcga tcacgatagt tgctaagttt agcagtctga     5400

gagtttggct gtgacgcaag accgagagag agacagagag ttgtcccagt ttgagtactc     5460

gtaccgtact acagagtaaa tcgaaatcga gcgctcactt tctgcacttt gtgaaggcgc     5520

agcattcaac atttgtgtcc agcccctttc cgtttgaagc gacttgacca cgaaatgttg     5580

gaataaagct gtgttacata cgagacctac aagtagagtg tcggatatgc caactgaaca     5640

ggttagatga aagttactca acaacaggag agcggacacc aacgtgcgaa aatgtacgga     5700

tgaacccatt gtctgtccgc atagcaatct gttttcgcca gcagtgcttg tgtcgttgga     5760

ttgctgccat tgtactattt gtactttgtt ccattaacac tcgcatcttg ctcaccgatt     5820

agcagcactc ctgttgcctc actgggcttg tctgtttcag ggtggaatgc atgagcaatg     5880

cgatcgtcaa ggggaggttg aaagcgcgta tggaataact gtggttctat agctgagggg     5940

aggatatcga ggacagccac ggttcagtgg gggatttcgg gagctactca gtaacatgta     6000

ctcttgtccg aacgccctgc tgatggcaaa tatgacaaaa cgcaacaatg tggagcaaca     6060

tttccatttc gagagatatt acatgggttg cattggggcc tgggcatccc gtgcaataac     6120

cgagaccatg tgaaggtgca cgatcgacac ttatccagcc agtcatacgt ggtgtcgcaa     6180

gctaagcaac aatgggattc acgaatctat gtgggggaac gtgcacaggg gaaatgaaac     6240

aaaacggagg agggggtctt tgaaaatgta gagaaatata acaaaaaatc acaaaaacgc     6300

accaaaaata tgccaaaatc gtcaacagtg gaaatcacgc gcccctggct cgacttccca     6360

ctgacatacc ccccctcaac caatatcata tccaccctag ccaatcgctt aattaagttg     6420

cgacacatgt cttgatagta tcttgaattc tctctcttga gcttttccat aacaagttct     6480

tctgcctcca ggaagtccat gggtggtttg atcatggttt tggtgtagtg gtagtgcagt     6540

ggtggtattg tgactgggga tgtagttgag aataagtcat acacaagtca gctttcttcg     6600

agcctcatat aagtataagt agttcaacgt attagcactg tacccagcat ctccgtatcg     6660

agaaacacaa caacatgccc cattggacag atcatgcgga tacacaggtt gtgcagtatc     6720

atacatactc gatcagacag gtcgtctgac catcatacaa gctgaacaag cgctccatac     6780

ttgcacgctc tctatataca cagttaaatt acatatccat agtctaacct ctaacagtta     6840

atcttctggt aagcctccca gccagccttc tggtatcgct tggcctcctc aataggatct     6900

cggttctggc cgtacagacc tcggccgaca attatgatat ccgttccggt agacatgaca     6960

tcctcaacag ttcggtactg ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg     7020

ggggtcagaa taagccagtc ctcagagtcg cccttaggtc ggttctgggc aatgaagcca     7080

accacaaact cggggtcgga tcgggcaagc tcaatggtct gcttggagta ctcgccagtg     7140

gccagagagc ccttgcaaga cagctcggcc agcatgagca gacctctggc cagcttctcg     7200

ttgggagagg ggactaggaa ctccttgtac tgggagttct cgtagtcaga gacgtcctcc     7260

ttcttctgtt cagagacagt ttcctcggca ccagctcgca ggccagcaat gattccggtt     7320

ccgggtacac cgtgggcgtt ggtgatatcg gaccactcgg cgattcggtg acaccggtac     7380

tggtgcttga cagtgttgcc aatatctgcg aactttctgt cctcgaacag gaagaaaccg     7440

tgcttaagag caagttcctt gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg     7500

atgtcgatat gggttttgat catgcacaca taaggtccga ccttatcggc aagctcaatg     7560

agctccttgg tggtggtaac atccagagaa gcacacaggt tggttttctt ggctgccacg     7620

agcttgagca ctcgagcggc aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag     7680

ggcattttgg tggtgaagag gagactgaaa taaatttagt ctgcagaact ttttatcgga     7740

accttatctg gggcagtgaa gtatatgtta tggtaatagt tacgagttag ttgaacttat     7800

agatagactg gactatacgg ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg     7860

gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc cagcaatgac gttgcagctg     7920

atattgttgt cggccaaccg cgccgaaaac gcagctgtca gacccacagc ctccaacgaa     7980

gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg agtcgtactc caaaggcggc     8040

aatgacgagt cagacagata ctcgtcgacc ttttccttgg gaaccaccac cgtcagccct     8100

tctgactcac gtattgtagc caccgacaca ggcaacagtc cgtggatagc agaatatgtc     8160

ttgtcggtcc atttctcacc aactttaggc gtcaagtgaa tgttgcagaa gaagtatgtg     8220

ccttcattga gaatcggtgt tgctgatttc aataaagtct tgagatcagt ttggccagtc     8280

atgttgtggg gggtaattgg attgagttat cgcctacagt ctgtacaggt atactcgctg     8340

cccactttat actttttgat tccgctgcac ttgaagcaat gtcgtttacc aaaagtgaga     8400

atgctccaca gaacacaccc cagggtatgg ttgagcaaaa aataaacact ccgatacggg     8460

gaatcgaacc ccggtctcca cggttctcaa gaagtattct tgatgagagc gtatcgatag     8520

ttggagcaag ggagaaatgt agagtgtgaa agactcacta tggtccgggc ttatctcgac     8580

caatagccaa agtctggagt ttctgagaga aaaaggcaag atacgtatgt aacaaagcga     8640

cgcatggtac aataataccg gaggcatgta tcatagagag ttagtggttc gatgatggca     8700

ctggtgcctg gtatgacttt atacggctga ctacatattt gtcctcagac atacaattac     8760

agtcaagcac ttacccttgg acatctgtag gtaccccccg gccaagacga tctcagcgtg     8820

tcgtatgtcg gattggcgta gctccctcgc tcgtcaattg gctcccatct actttcttct     8880

gcttggctac acccagcatg tctgctatgg ctcgttttcg tgccttatct atcctcccag     8940

tattaccaac tctaaatgac atgatgtgat tgggtctaca ctttcatatc agagataagg     9000

agtagcacag ttgcataaaa agcccaactc taatcagctt cttcctttct tgtaattagt     9060

acaaaggtga ttagcgaaat ctggaagctt agttggccct aaaaaaatca aaaaaagcaa     9120

aaaacgaaaa acgaaaaacc acagttttga gaacagggag gtaacgaagg atcgtatata     9180

tatatatata tatatatacc cacggatccc gagaccggcc tttgattctt ccctacaacc     9240

aaccattctc accaccctaa ttcacaacca tgtctattgg ttcgtccaac cccgtgctct     9300

tggctgcgat tcccttcgtc tacctgtttg tcctcccacg agtcctggct ttcctgcctc     9360

agaaggctca gttcctggcc aaatgtattg tggtcctgat tgccacgctt atcatgtccg     9420

ttgcaggctg cttcatctcg atcgtgtgcg ctcttctgga caagagatac gtcatcaatt     9480

acgttgtgtc gcgattgttc tccttccttg ccgctcgacc gtgtggtgtg acctataaga     9540

ttgttggtga ggaacacctc gataagtacc ctgctatcgt ggtctgtaac catcaatcct     9600

ctatggatat gatggttttg ggacgagttt ttccaaagca ctgcgttgtc atggcgaaga     9660

aggaactcct gtactttccc tttttgggaa tgtttatgaa actgagcaac gctatcttca     9720

tcgaccggaa gaaccacaag aaagccatcg agtctaccac ccaagccgtg gcggacatga     9780

agaagcacaa ctctggaatc tggattttcc cagagggcac ccggtctaga ctggacaagg     9840

cagacctgct gcccttcaag aaaggtgcct ttcatcttgc aattcaggcc cagctcccta     9900

ttctccccat tatctcgcag ggctattccc atatctacga ctcttcgaag cggtacttcc     9960

ccggtggaga gctcgagatc agagtcctgg agcccattcc tacaactggc ctcactactg    10020

atgatgtgaa cgacctgatg gacaagacac gaaaccttat gctcaagcac ttgaaggaga    10080

tggattccca gtattcgtcg agcactgctg aaaatggatc cacgcacatc gacgccgata    10140

ttgccaagtc tacagccacc agcattggca acactgacga cgcaattaca aaacgtcgta    10200

cccctaagga ataagcggcc gcattgatga ttggaaacac acacatgggt tatatctagg    10260

tgagagttag ttggacagtt atatattaaa tcagctatgc caacggtaac ttcattcatg    10320

tcaacgagga accagtgact gcaagtaata tagaatttga ccaccttgcc attctcttgc    10380

actcctttac tatatctcat ttatttctta tatacaaatc acttcttctt cccagcatcg    10440

agctcggaaa cctcatgagc aataacatcg tggatctcgt caatagaggg ctttttggac    10500

tccttgctgt tggccacctt gtccttgctg tttaaaccat catctaaggg cctcaaaact    10560

acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac    10620

caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac    10680

aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg    10740

cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat    10800

gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt    10860

tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca    10920

accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata    10980

tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga    11040

ttcgaaatct aaactacaca tcacaccatg gtctccaacc acctgttcga cgccatgcga    11100

gctgccgctc ccggagacgc acctttcatt cgaatcgaca acgctcggac ctggacttac    11160

gatgacgcca ttgctctttc cggtcgaatc gctggagcta tggacgcact cggcattcga    11220

cccggagaca gagttgccgt gcaggtcgag aagtctgccg aggcgttgat tctctacctg    11280

gcctgtcttc gaaccggagc tgtctacctg cctctcaaca ctgcctacac cctggccgag    11340

ctcgactact tcatcggcga tgccgaaccg cgtctggtgg tcgttgctcc cgcagctcga    11400

ggtggcgtgg agacaattgc caagcgacac ggtgctatcg tcgaaaccct cgacgccgat    11460

ggacgaggct ccttgctgga ccttgctaga gatgagcctg ccgactttgt cgatgcttcg    11520

cgatctgccg acgatctggc tgctattctc tacacttccg gtacaaccgg acgatcgaag    11580

ggtgccatgc ttactcatgg caatctgctc tccaacgctc tcaccttgcg agactattgg    11640

agagttaccg cagacgatcg actcatccat gccttgccaa tctttcacac tcatggtctg    11700

ttcgttgcta cgaacgtcac actgcttgca ggagcctcga tgtttctgct ctccaagttc    11760

gatgccgacg aggtcgtttc tctcatgcca caggccacca tgcttatggg cgtgcccaca    11820

ttctacgttc gattgctgca gagtcctcga ctcgagaagg gtgctgtggc cagcatcaga    11880

ctgttcattt ctggatcagc tcccttgctt gccgaaaccc acgccgagtt tcatgctcgt    11940

actggtcacg ccattctcga gcgatacggc atgacggaaa ccaacatgaa tacttccaac    12000

ccctacgagg gcaagcgtat tgccggaacc gttggttttc ctctgcccga cgtcactgtg    12060

cgagtcaccg atcccgccac cggtctcgtt cttccacctg aagagactgg catgatcgag    12120

atcaagggac ccaacgtctt caagggctat tggcgaatgc ccgaaaagac cgctgccgag    12180

tttaccgcag acggtttctt tatctctgga gatctcggca agatcgaccg agaaggttac    12240

gttcacattg tgggacgagg caaggacctg gtcatttccg gtggctacaa catctatccc    12300

aaagaggtcg aaggcgagat cgaccagatc gagggtgtgg tcgagtctgc tgtcattggt    12360

gttcctcatc ccgatttcgg agaaggtgtc accgctgttg tcgtgtgcaa acctggtgcc    12420

gttctcgacg aaaagaccat cgtgtctgct ctgcaggacc gtcttgcccg atacaagcaa    12480

cccaagcgga ttatctttgc cgacgatctg cctcgaaaca ctatgggaaa ggttcagaag    12540

aacattcttc gacagcaata cgccgatctc tacaccagac gataagcggc cgcatgagaa    12600

gataaatata taaatacatt gagatattaa atgcgctaga ttagagagcc tcatactgct    12660

cggagagaag ccaagacgag tactcaaagg ggattacacc atccatatcc acagacacaa    12720

gctggggaaa ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg    12780

gggcagccag gatttcaggc acttcggtgt ctcggggtga aatggcgttc ttggcctcca    12840

tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga tgaagaatga    12900

acttgaagtg aaggaattta aatgtaacga aactgaaatt tgaccagata ttgtgtccgc    12960

ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat    13020

ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aagcttccac acaac         13075


<210>  138
<211>  15799
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL2-5m89C

<400>  138
gtacgttatc atttgaacag tgaaaggcta cagtaacaga agcagttgta aacttcattc       60

cgttgattct gtactacagt accccactac gccgcttccg ctgacactgt tcaacccaaa      120

aactacatct gcgtgcgctg tgtaaggcta tcatcagata catactgtag attctgtaga      180

tgcgaacctg cttgtatcat atacatcccc ctccccctga cctgcacaag caagcaatgt      240

gacattgata ttgctgctta tctagtgccg aggatgtgaa agccgagact caaacatttc      300

ttttactctc ttgttcctga ccagacctgg cggagattac gccagtatga ttcttgcagg      360

tctgagacaa gcctggaaca gccaacattt atttttcgaa gcgagaaaca tgccacaccc      420

cggcacgttc agagatgcat atgatttgtt tttcgagtaa cagtaccccc cccccccccc      480

ccaatgaaac cagtattact cacaccatcc tcattcaaag cgttacactg attacgcgcc      540

catcaacgac agcatgaggg gactgctgat ctgatctaat caaatgacta caaaaatcgc      600

aataatgaag agcaaacgac aaaaaagaaa caggttaacc aatcccgctt caatgtctca      660

ccacaatcca gcactgtttc tcattacctc ctccctctaa tttcagagtt gcatcagggt      720

ccttgatggc gcgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc      780

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc      840

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata      900

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg      960

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct     1020

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa     1080

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc     1140

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt     1200

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg     1260

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg     1320

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct     1380

tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc     1440

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     1500

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     1560

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     1620

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     1680

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat     1740

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct     1800

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg     1860

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag     1920

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta     1980

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg     2040

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg     2100

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct     2160

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta     2220

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg     2280

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc     2340

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg     2400

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga     2460

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg     2520

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat     2580

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc     2640

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca     2700

catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga     2760

aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt     2820

gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa     2880

aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa     2940

agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac     3000

gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga     3060

accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa     3120

aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc     3180

tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc     3240

attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca     3300

gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca     3360

gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt     3420

gggcccgacg tcgcatgctg gtttcgattt gtcttagagg aacgcatata cagtaatcat     3480

agagaataaa cgatattcat ttattaaagt agatagttga ggtagaagtt gtaaagagtg     3540

ataaatagct tagataccac agacaccctc ggtgacgaag tactgcagat ggtttccaat     3600

cacattgacc tgctggagca gagtgttacc ggcagagcac tgtttattgc tctggccctg     3660

gcacatgaca acgttggaga gaggagggtg gatcaggggc cagtcaataa agacctcacc     3720

agagcagtgc tggtaaccgt cccagaaggg cacttgaggg acgatatctc ctcggtgggt     3780

gattcggtag agctttcggt ctttggacac cttggagaca tcggggttct cctggccaaa     3840

gaagagttta tcgacccagt tagcaaagcc agcgttaccg acaatgggct gaccaagagt     3900

aacaacgagg ggatcgtggc cgttaacctt gaggttgatt ccgaacagaa gggctgcagc     3960

tcctccgaga gagtgaccgg tgacagcaat ctggtagtcg ggatactgct caatcacaga     4020

gtcgagcttg gggccgatct gattgtaggt gttgttgtag gactggatga agccattgtg     4080

gacaagacag tcatcacaag tagcagtaga agagatgtta gcagcaagat caaagttaat     4140

taactcacct gcaggattga gactatgaat ggattcccgt gcccgtatta ctctactaat     4200

ttgatcttgg aacgcgaaaa tacgtttcta ggactccaaa gaatctcaac tcttgtcctt     4260

actaaatata ctacccatag ttgatggttt acttgaacag agaggacatg ttcacttgac     4320

ccaaagtttc tcgcatctct tggatatttg aacaacggcg tccactgacc gtcagttatc     4380

cagtcacaaa acccccacat tcatacattc ccatgtacgt ttacaaagtt ctcaattcca     4440

tcgtgcaaat caaaatcaca tctattcatt catcatatat aaacccatca tgtctactaa     4500

cactcacaac tccatagaaa acatcgactc agaacacacg ctccatgcgg ccgcttagga     4560

atcctgagcg tccttgacac agtgaaccac accgactttg tgcatgtact tgagggtgga     4620

aatgatgttg cccacaatgg tagggtagaa gacgtaccga actccgtgtc gttcgcaaca     4680

ctctcggaca gcttgctgca cgaagggata gtgccaagac gacattcgag gaaagaggtg     4740

atgctcgatc tggaagttga gaccgccagt aaagaacatg gcaatgggtc caccgtaggt     4800

ggaagaggtc tccacctgag ctctgtacca gtcgatctga tcggcttcaa cgtccttctc     4860

ggagctcttg accttgcagt tcttgtcggg gattcgctcc gagccatcga agttgtgaga     4920

caagatgaaa aagaaggtga ggaaggcacc ggtagcagtg ggcaccagag gaatggtgat     4980

gagcagggag gttccagtga gataccaggg caagaaggcg gttcgaaaga tgaagaaagc     5040

tcgcataacg aatgcaaggg ttcggtaccg tcgcagaaag ccgttctctc gcatggctgt     5100

gacagactcg ggaatggtgt cgttgtgctg cattcggaag atgtagagag ggttgtacac     5160

cagcgaaacg ccgtaggctc caagcacgag gtacatgtac caggcctgga atcggtgaaa     5220

ccactttcga gcagtgttgg cagcagggta gttgtggaac acaaggaatg gttctgcgga     5280

ctcggcatcc aggtcgagac catgctgatt ggtgtaggtg tgatgtcgca tgatgtgaga     5340

ctgcagccag atccatctgg acgatccaat gacgtcgatg ccgtaggcaa agagagcgtt     5400

gacccagggc tttttgctga tggcaccatg agaggcatcg tgctgaatgg acaggccgat     5460

ctgcatgtgc atgaatccag tcaagagacc ccacagcacc attccggtag tagcccagtg     5520

ccactcgcaa aaggcggtga cagcaatgat gccaacggtt cgcagccaga atccaggtgt     5580

ggcataccag ttccgacctt tcatgacctc tcgcatagtt cgcttgacgt cctgtgcaaa     5640

gggagagtcg taggtgtaga caatgtcctt ggaggttcgg tcgtgcttgc ctcgcacgaa     5700

ctgttgaagc agcttcgagt tctcgggctt gacgtaaggg tgcatggagt agaacagagg     5760

agaagcatcg gaggcaccag aagcgaggat caagtcggat ccgggatgga ccttggcaag     5820

accttccaga tcgtagagaa tgccgtcgat ggcaaccagg tcgggtcgct cgagcagctg     5880

ctcggtagta agggagagag ccatggttgt gaattagggt ggtgagaatg gttggttgta     5940

gggaagaatc aaaggccggt ctcgggatcc gtgggtatat atatatatat atatatatac     6000

gatccttcgt tacctccctg ttctcaaaac tgtggttttt cgtttttcgt tttttgcttt     6060

ttttgatttt tttagggcca actaagcttc cagatttcgc taatcacctt tgtactaatt     6120

acaagaaagg aagaagctga ttagagttgg gctttttatg caactgtgct actccttatc     6180

tctgatatga aagtgtagac ccaatcacat catgtcattt agagttggta atactgggag     6240

gatagataag gcacgaaaac gagccatagc agacatgctg ggtgtagcca agcagaagaa     6300

agtagatggg agccaattga cgagcgaggg agctacgcca atccgacata cgacacgctg     6360

agatcgtctt ggccgggggg tacctacaga tgtccaaggg taagtgcttg actgtaattg     6420

tatgtctgag gacaaatatg tagtcagccg tataaagtca taccaggcac cagtgccatc     6480

atcgaaccac taactctcta tgatacatgc ctccggtatt attgtaccat gcgtcgcttt     6540

gttacatacg tatcttgcct ttttctctca gaaactccag aattctctct cttgagcttt     6600

tccataacaa gttcttctgc ctccaggaag tccatgggtg gtttgatcat ggttttggtg     6660

tagtggtagt gcagtggtgg tattgtgact ggggatgtag ttgagaataa gtcatacaca     6720

agtcagcttt cttcgagcct catataagta taagtagttc aacgtattag cactgtaccc     6780

agcatctccg tatcgagaaa cacaacaaca tgccccattg gacagatcat gcggatacac     6840

aggttgtgca gtatcataca tactcgatca gacaggtcgt ctgaccatca tacaagctga     6900

acaagcgctc catacttgca cgctctctat atacacagtt aaattacata tccatagtct     6960

aacctctaac agttaatctt ctggtaagcc tcccagccag ccttctggta tcgcttggcc     7020

tcctcaatag gatctcggtt ctggccgtac agacctcggc cgacaattat gatatccgtt     7080

ccggtagaca tgacatcctc aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg     7140

tcaagaccca ccccgggggt cagaataagc cagtcctcag agtcgccctt aggtcggttc     7200

tgggcaatga agccaaccac aaactcgggg tcggatcggg caagctcaat ggtctgcttg     7260

gagtactcgc cagtggccag agagcccttg caagacagct cggccagcat gagcagacct     7320

ctggccagct tctcgttggg agaggggact aggaactcct tgtactggga gttctcgtag     7380

tcagagacgt cctccttctt ctgttcagag acagtttcct cggcaccagc tcgcaggcca     7440

gcaatgattc cggttccggg tacaccgtgg gcgttggtga tatcggacca ctcggcgatt     7500

cggtgacacc ggtactggtg cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg     7560

aacaggaaga aaccgtgctt aagagcaagt tccttgaggg ggagcacagt gccggcgtag     7620

gtgaagtcgt caatgatgtc gatatgggtt ttgatcatgc acacataagg tccgacctta     7680

tcggcaagct caatgagctc cttggtggtg gtaacatcca gagaagcaca caggttggtt     7740

ttcttggctg ccacgagctt gagcactcga gcggcaaagg cggacttgtg gacgttagct     7800

cgagcttcgt aggagggcat tttggtggtg aagaggagac tgaaataaat ttagtctgca     7860

gaacttttta tcggaacctt atctggggca gtgaagtata tgttatggta atagttacga     7920

gttagttgaa cttatagata gactggacta tacggctatc ggtccaaatt agaaagaacg     7980

tcaatggctc tctgggcgtc gcctttgccg acaaaaatgt gatcatgatg aaagccagca     8040

atgacgttgc agctgatatt gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc     8100

acagcctcca acgaagaatg tatcgtcaaa gtgatccaag cacactcata gttggagtcg     8160

tactccaaag gcggcaatga cgagtcagac agatactcgt cgaccttttc cttgggaacc     8220

accaccgtca gcccttctga ctcacgtatt gtagccaccg acacaggcaa cagtccgtgg     8280

atagcagaat atgtcttgtc ggtccatttc tcaccaactt taggcgtcaa gtgaatgttg     8340

cagaagaagt atgtgccttc attgagaatc ggtgttgctg atttcaataa agtcttgaga     8400

tcagtttggc cagtcatgtt gtggggggta attggattga gttatcgcct acagtctgta     8460

caggtatact cgctgcccac tttatacttt ttgattccgc tgcacttgaa gcaatgtcgt     8520

ttaccaaaag tgagaatgct ccacagaaca caccccaggg tatggttgag caaaaaataa     8580

acactccgat acggggaatc gaaccccggt ctccacggtt ctcaagaagt attcttgatg     8640

agagcgtatc gatcgaggaa gaggacaagc ggctgcttct taagtttgtg acatcagtat     8700

ccaaggcacc attgcaagga ttcaaggctt tgaacccgtc atttgccatt cgtaacgctg     8760

gtagacaggt tgatcggttc cctacggcct ccacctgtgt caatcttctc aagctgcctg     8820

actatcagga cattgatcaa cttcggaaga aacttttgta tgccattcga tcacatgctg     8880

gtttcgattt gtcttagagg aacgcatata cagtaatcat agagaataaa cgatattcat     8940

ttattaaagt agatagttga ggtagaagtt gtaaagagtg ataaatagcg gccgctcact     9000

gaatcttttt ggctcccttg tgctttcgga cgatgtaggt ctgcacgtag aagttgagga     9060

acagacacag gacagtacca acgtagaagt agttgaaaaa ccagccaaac attctcattc     9120

catcttgtcg gtagcaggga atgttccggt acttccagac gatgtagaag ccaacgttga     9180

actgaatgat ctgcatagaa gtaatcaggg acttgggcat agggaacttg agcttgatca     9240

gtcgggtcca atagtagccg tacatgatcc agtgaatgaa gccgttgagc agcacaaaga     9300

tccaaacggc ttcgtttcgg tagttgtaga acagccacat gtccatagga gctccgagat     9360

ggtgaaagaa ctgcaaccag gtcagaggct tgcccatgag gggcagatag aaggagtcaa     9420

tgtactcgag gaacttgctg aggtagaaca gctgagtggt gattcggaag acattgttgt     9480

cgaaagcctt ctcgcagttg tcggacatga caccaatggt gtacatggcg taggccatag     9540

agaggaagga gcccagcgag tagatggaca tgagcaggtt gtagttggtg aacacaaact     9600

tcattcgaga ctgacccttg ggtccgagag gaccaagggt gaacttcagg atgacgaagg     9660

cgatggagag gtacagcacc tcgcagtgcg aggcatcaga ccagagctga gcatagtcga     9720

ccttgggaag aacctcctgg ccaatggaga cgatttcgtt cacgacctcc atggttgtga     9780

attagggtgg tgagaatggt tggttgtagg gaagaatcaa aggccggtct cgggatccgt     9840

gggtatatat atatatatat atatatacga tccttcgtta cctccctgtt ctcaaaactg     9900

tggtttttcg tttttcgttt tttgcttttt ttgatttttt tagggccaac taagcttcca     9960

gatttcgcta atcacctttg tactaattac aagaaaggaa gaagctgatt agagttgggc    10020

tttttatgca actgtgctac tccttatctc tgatatgaaa gtgtagaccc aatcacatca    10080

tgtcatttag agttggtaat actgggagga tagataaggc acgaaaacga gccatagcag    10140

acatgctggg tgtagccaag cagaagaaag tagatgggag ccaattgacg agcgagggag    10200

ctacgccaat ccgacatacg acacgctgag atcgtcttgg ccggggggta cctacagatg    10260

tccaagggta agtgcttgac tgtaattgta tgtctgagga caaatatgta gtcagccgta    10320

taaagtcata ccaggcacca gtgccatcat cgaaccacta actctctatg atacatgcct    10380

ccggtattat tgtaccatgc gtcgctttgt tacatacgta tcttgccttt ttctctcaga    10440

aactccagac tttggctatt ggtcgagata agcccggacc atagtgagtc tttcacactc    10500

tgtttaaaca ccactaaaac cccacaaaat atatcttacc gaatatacag atctactata    10560

gaggaacaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca    10620

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc    10680

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg    10740

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac    10800

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac    10860

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca    10920

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa    10980

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct    11040

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca    11100

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt    11160

ttttgccttc cgcacatttc cattgctcgg tacccacacc ttgcttctcc tgcacttgcc    11220

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat    11280

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag    11340

attcgaaatc taaactacac atcacacaat gcctgttact gacgtcctta agcgaaagtc    11400

cggtgtcatc gtcggcgacg atgtccgagc cgtgagtatc cacgacaaga tcagtgtcga    11460

gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct agcaacacac actctctaca    11520

caaactaacc cagctctcca tggtgaaggc ttctcgacag gctctgcccc tcgtcatcga    11580

cggaaaggtg tacgacgtct ccgcttgggt gaacttccac cctggtggag ctgaaatcat    11640

tgagaactac cagggacgag atgctactga cgccttcatg gttatgcact ctcaggaagc    11700

cttcgacaag ctcaagcgaa tgcccaagat caaccaggct tccgagctgc ctccccaggc    11760

tgccgtcaac gaagctcagg aggatttccg aaagctccga gaagagctga tcgccactgg    11820

catgtttgac gcctctcccc tctggtactc gtacaagatc ttgaccaccc tgggtcttgg    11880

cgtgcttgcc ttcttcatgc tggtccagta ccacctgtac ttcattggtg ctctcgtgct    11940

cggtatgcac taccagcaaa tgggatggct gtctcatgac atctgccacc accagacctt    12000

caagaaccga aactggaata acgtcctggg tctggtcttt ggcaacggac tccagggctt    12060

ctccgtgacc tggtggaagg acagacacaa cgcccatcat tctgctacca acgttcaggg    12120

tcacgatccc gacattgata acctgcctct gctcgcctgg tccgaggacg atgtcactcg    12180

agcttctccc atctcccgaa agctcattca gttccaacag tactatttcc tggtcatctg    12240

tattctcctg cgattcatct ggtgtttcca gtctgtgctg accgttcgat ccctcaagga    12300

ccgagacaac cagttctacc gatctcagta caagaaagag gccattggac tcgctctgca    12360

ctggactctc aagaccctgt tccacctctt ctttatgccc tccatcctga cctcgatgct    12420

ggtgttcttt gtttccgagc tcgtcggtgg cttcggaatt gccatcgtgg tcttcatgaa    12480

ccactaccct ctggagaaga tcggtgattc cgtctgggac ggacatggct tctctgtggg    12540

tcagatccat gagaccatga acattcgacg aggcatcatt actgactggt tctttggagg    12600

cctgaactac cagatcgagc accatctctg gcccaccctg cctcgacaca acctcactgc    12660

cgtttcctac caggtggaac agctgtgcca gaagcacaac ctcccctacc gaaaccctct    12720

gccccatgaa ggtctcgtca tcctgctccg atacctgtcc cagttcgctc gaatggccga    12780

gaagcagccc ggtgccaagg ctcagtaagc ggccgcatga gaagataaat atataaatac    12840

attgagatat taaatgcgct agattagaga gcctcatact gctcggagag aagccaagac    12900

gagtactcaa aggggattac accatccata tccacagaca caagctgggg aaaggttcta    12960

tatacacttt ccggaatacc gtagtttccg atgttatcaa tgggggcagc caggatttca    13020

ggcacttcgg tgtctcgggg tgaaatggcg ttcttggcct ccatcaagtc gtaccatgtc    13080

ttcatttgcc tgtcaaagta aaacagaagc agatgaagaa tgaacttgaa gtgaaggaat    13140

ttaaatgatg tcgacgcagt aggatgtcct gcacgggtct ttttgtgggg tgtggagaaa    13200

ggggtgcttg gatcgatgga agccggtaga accgggctgc ttgtgcttgg agatggaagc    13260

cggtagaacc gggctgcttg gggggatttg gggccgctgg gctccaaaga ggggtaggca    13320

tttcgttggg gttacgtaat tgcggcattt gggtcctgcg cgcatgtccc attggtcaga    13380

attagtccgg ataggagact tatcagccaa tcacagcgcc ggatccacct gtaggttggg    13440

ttgggtggga gcacccctcc acagagtaga gtcaaacagc agcagcaaca tgatagttgg    13500

gggtgtgcgt gttaaaggaa aaaaaagaag cttgggttat attcccgctc tatttagagg    13560

ttgcgggata gacgccgacg gagggcaatg gcgctatgga accttgcgga tatccatacg    13620

ccgcggcgga ctgcgtccga accagctcca gcagcgtttt ttccgggcca ttgagccgac    13680

tgcgaccccg ccaacgtgtc ttggcccacg cactcatgtc atgttggtgt tgggaggcca    13740

ctttttaagt agcacaaggc acctagctcg cagcaaggtg tccgaaccaa agaagcggct    13800

gcagtggtgc aaacggggcg gaaacggcgg gaaaaagcca cgggggcacg aattgaggca    13860

cgccctcgaa tttgagacga gtcacggccc cattcgcccg cgcaatggct cgccaacgcc    13920

cggtcttttg caccacatca ggttacccca agccaaacct ttgtgttaaa aagcttaaca    13980

tattataccg aacgtaggtt tgggcgggct tgctccgtct gtccaaggca acatttatat    14040

aagggtctgc atcgccggct caattgaatc ttttttcttc ttctcttctc tatattcatt    14100

cttgaattaa acacacatca accatgggcg tattcattaa acaggagcag cttccggctc    14160

tcaagaagta caagtactcc gccgaggatc actcgttcat ctccaacaac attctgcgcc    14220

ccttctggcg acagtttgtc aaaatcttcc ctctgtggat ggcccccaac atggtgactc    14280

tgctgggctt cttctttgtc attgtgaact tcatcaccat gctcattgtt gatcccaccc    14340

acgaccgcga gcctcccaga tgggtctacc tcacctacgc tctgggtctg ttcctttacc    14400

agacatttga tgcctgtgac ggatcccatg cccgacgaac tggccagagt ggaccccttg    14460

gagagctgtt tgaccactgt gtcgacgcca tgaatacctc tctgattctc acggtggtgg    14520

tgtccaccac ccatatggga tataacatga agctactgat tgtgcagatt gccgctctcg    14580

gaaacttcta cctgtcgacc tgggagacct accataccgg aactctgtac ctttctggct    14640

tctctggtcc tgttgaaggt atcttgattc tggtggctct tttcgtcctc accttcttca    14700

ctggtcccaa cgtgtacgct ctgaccgtct acgaggctct tcccgagtcc atcacttcgc    14760

tgctgcctgc cagcttcctg gacgtcacca tcacccagat ctacattgga ttcggagtgc    14820

tgggcatggt gttcaacatc tacggcgcct gcggaaacgt gatcaagtac tacaacaaca    14880

agggcaagag cgctctcccc gccattctcg gaatcgcccc ctttggcatc ttctacgtcg    14940

gcgtctttgc ctgggcccat gttgctcctc tgcttctctc caagtacgcc atcgtctatc    15000

tgtttgccat tggggctgcc tttgccatgc aagtcggcca gatgattctt gcccatctcg    15060

tgcttgctcc ctttccccac tggaacgtgc tgctcttctt cccctttgtg ggactggcag    15120

tgcactacat tgcacccgtg tttggctggg acgccgatat cgtgtcggtt aacactctct    15180

tcacctgttt tggcgccacc ctctccattt acgccttctt tgtgcttgag atcatcgacg    15240

agatcaccaa ctacctcgat atctggtgtc tgcgaatcaa gtaccctcag gagaagaaga    15300

ccgaataagc ggccgcatgg agcgtgtgtt ctgagtcgat gttttctatg gagttgtgag    15360

tgttagtaga catgatgggt ttatatatga tgaatgaata gatgtgattt tgatttgcac    15420

gatggaattg agaactttgt aaacgtacat gggaatgtat gaatgtgggg gttttgtgac    15480

tggataactg acggtcagtg gacgccgttg ttcaaatatc caagagatgc gagaaacttt    15540

gggtcaagtg aacatgtcct ctctgttcaa gtaaaccatc aactatgggt agtatattta    15600

gtaaggacaa gagttgagat tctttggagt cctagaaacg tattttcgcg ttccaagatc    15660

aaattagtag agtaatacgg gcacgggaat ccattcatag tctcaatcct gcaggtgagt    15720

taattaatcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc    15780

gctcacaatt ccacacaac                                                 15799


<210>  139
<211>  9641
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY201

<400>  139
gtacgataac ttcgtatagc atacattata cgaagttatc gcgtcgacga gtatctgtct       60

gactcgtcat tgccgccttt ggagtacgac tccaactatg agtgtgcttg gatcactttg      120

acgatacatt cttcgttgga ggctgtgggt ctgacagctg cgttttcggc gcggttggcc      180

gacaacaata tcagctgcaa cgtcattgct ggctttcatc atgatcacat ttttgtcggc      240

aaaggcgacg cccagagagc cattgacgtt ctttctaatt tggaccgata gccgtatagt      300

ccagtctatc tataagttca actaactcgt aactattacc ataacatata cttcactgcc      360

ccagataagg ttccgataaa aagttctgca gactaaattt atttcagtct cctcttcacc      420

accaaaatgc cctcctacga agctcgagct aacgtccaca agtccgcctt tgccgctcga      480

gtgctcaagc tcgtggcagc caagaaaacc aacctgtgtg cttctctgga tgttaccacc      540

accaaggagc tcattgagct tgccgataag gtcggacctt atgtgtgcat gatcaaaacc      600

catatcgaca tcattgacga cttcacctac gccggcactg tgctccccct caaggaactt      660

gctcttaagc acggtttctt cctgttcgag gacagaaagt tcgcagatat tggcaacact      720

gtcaagcacc agtaccggtg tcaccgaatc gccgagtggt ccgatatcac caacgcccac      780

ggtgtacccg gaaccggaat cattgctggc ctgcgagctg gtgccgagga aactgtctct      840

gaacagaaga aggaggacgt ctctgactac gagaactccc agtacaagga gttcctagtc      900

ccctctccca acgagaagct ggccagaggt ctgctcatgc tggccgagct gtcttgcaag      960

ggctctctgg ccactggcga gtactccaag cagaccattg agcttgcccg atccgacccc     1020

gagtttgtgg ttggcttcat tgcccagaac cgacctaagg gcgactctga ggactggctt     1080

attctgaccc ccggggtggg tcttgacgac aagggagacg ctctcggaca gcagtaccga     1140

actgttgagg atgtcatgtc taccggaacg gatatcataa ttgtcggccg aggtctgtac     1200

ggccagaacc gagatcctat tgaggaggcc aagcgatacc agaaggctgg ctgggaggct     1260

taccagaaga ttaactgtta gaggttagac tatggatatg taatttaact gtgtatatag     1320

agagcgtgca agtatggagc gcttgttcag cttgtatgat ggtcagacga cctgtctgat     1380

cgagtatgta tgatactgca caacctgtgt atccgcatga tctgtccaat ggggcatgtt     1440

gttgtgtttc tcgatacgga gatgctgggt acagtgctaa tacgttgaac tacttatact     1500

tatatgaggc tcgaagaaag ctgacttgtg tatgacttat tctcaactac atccccagtc     1560

acaataccac cactgcacta ccactacacc aaaaccatga tcaaaccacc catggacttc     1620

ctggaggcag aagaacttgt tatggaaaag ctcaagagag agatcataac ttcgtatagc     1680

atacattata cgaagttatc ctgcaggtaa aggaattcag gagagaccgg gttggcggcg     1740

tatttgtgtc ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc     1800

gggcccaacc ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac     1860

acttgccgtt aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta     1920

ctagtttctt tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa     1980

atttttttgc tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat     2040

tacctttcct cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt     2100

tccttctgag tataagaatc attcaccatg gacttcctgg aggcagaaga acttgttatg     2160

gaaaagctca agagagagaa gccaagatac tatcaagaca tgtgtcgcaa cttaattaag     2220

atgacgacat ttgcgagctg gacgaggaat agatggagcg tgtgttctga gtcgatgttt     2280

tctatggagt tgtgagtgtt agtagacatg atgggtttat atatgatgaa tgaatagatg     2340

tgattttgat ttgcacgatg gaattgagaa ctttgtaaac gtacatggga atgtatgaat     2400

gtgggggttt tgtgactgga taactgacgg tcagtggacg ccgttgttca aatatccaag     2460

agatgcgaga aactttgggt caagtgaaca tgtcctctct gttcaagtaa accatcaact     2520

atgggtagta tatttagtaa ggacaagagt tgagattctt tggagtccta gaaacgtatt     2580

ttcgcgttcc aagatcaaat tagtagagta atacgggcac gggaatccat tcatagtctc     2640

aattttccca taggtgtgct acaaggtgtt gagatgtggt acagtaccac catgattcga     2700

ggtaaagagc ccagaagtca ttgatgaggt caagaaatac acagatctac agctcaatac     2760

aatgaatatc ttctttcata ttcttcaggt gacaccaagg gtgtctattt tccccagaaa     2820

tgcgtgaaaa ggcgcgtgtg tagcgtggag tatgggttcg gttggcgtat ccttcatata     2880

tcgacgaaat agtagggcaa gagatgacaa aaagtatcta tatgtagaca gcgtagaata     2940

tggatttgat tggtataaat tcatttattg cgtgtctcac aaatactctc gataagttgg     3000

ggttaaactg gagatggaac aatgtcgata tctcgacgca tgcgacgtcg ggcccaattc     3060

gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga     3120

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg     3180

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga     3240

atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     3300

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     3360

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     3420

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     3480

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     3540

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     3600

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     3660

tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc     3720

ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc     3780

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     3840

taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc     3900

cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa     3960

acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa     4020

ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg     4080

atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa     4140

gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc     4200

acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc     4260

atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta     4320

accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag     4380

ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca     4440

acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata     4500

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     4560

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     4620

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     4680

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     4740

taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa     4800

tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt     4860

gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat     4920

cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg     4980

gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga     5040

gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac     5100

tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt     5160

ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag     5220

cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc     5280

gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag     5340

gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca     5400

gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt     5460

cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc     5520

tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc     5580

cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc     5640

cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa     5700

ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcgcgccac caatcacaat     5760

tctgaaaagc acatcttgat ctcctcattg cggggagtcc aacggtggtc ttattccccc     5820

gaatttcccg ctcaatctcg ttccagaccg acccggacac agtgcttaac gccgttccga     5880

aactctaccg cagatatgct ccaacggact gggctgcata gatgtgatcc tcggcttgga     5940

gaaatggata aaagccggcc aaaaaaaaag cggaaaaaag cggaaaaaaa gagaaaaaaa     6000

atcgcaaaat ttgaaaaata gggggaaaag acgcaaaaac gcaaggaggg gggagtatat     6060

gacactgata agcaagctca caacggttcc tcttattttt ttcctcatct tctgcctagg     6120

ttcccaaaat cccagatgct tctctccagt gccaaaagta agtaccccac aggttttcgg     6180

ccgaaaattc cacgtgcagc aacgtcgtgt ggggtgttaa aatgtggggg gggggaacca     6240

ggacaagagg ctcttgtggg agccgaatga gagcacaaag cgggcgggtg tgataagggc     6300

atttttgccc attttccctt ctcctgtctc tccgacggtg atggcgttgt gcgtcctcta     6360

tttcttttta tttctttttg ttttatttct ctgactaccg atttggtttg atttcctcaa     6420

ccccacacaa ataagctcgg gccgaggaat atatatatac acggacacag tcgccctgtg     6480

gacaacacgt cactacctct acgatacaca ccgtacgata gttagtagac aacaatcgat     6540

agttggagca agggagaaat gtagagtgtg aaagactcac tatggtccgg gcttatctcg     6600

accaatagcc aaagtctgga gtttctgaga gaaaaaggca agatacgtat gtaacaaagc     6660

gacgcatggt acaataatac cggaggcatg tatcatagag agttagtggt tcgatgatgg     6720

cactggtgcc tggtatgact ttatacggct gactacatat ttgtcctcag acatacaatt     6780

acagtcaagc acttaccctt ggacatctgt aggtaccccc cggccaagac gatctcagcg     6840

tgtcgtatgt cggattggcg tagctccctc gctcgtcaat tggctcccat ctactttctt     6900

ctgcttggct acacccagca tgtctgctat ggctcgtttt cgtgccttat ctatcctccc     6960

agtattacca actctaaatg acatgatgtg attgggtcta cactttcata tcagagataa     7020

ggagtagcac agttgcataa aaagcccaac tctaatcagc ttcttccttt cttgtaatta     7080

gtacaaaggt gattagcgaa atctggaagc ttagttggcc ctaaaaaaat caaaaaaagc     7140

aaaaaacgaa aaacgaaaaa ccacagtttt gagaacaggg aggtaacgaa ggatcgtata     7200

tatatatata tatatatata cccacggatc ccgagaccgg cctttgattc ttccctacaa     7260

ccaaccattc tcaccaccct aattcacaac catgtacaac cccgtggacg cagtgttgac     7320

taagattatt acaaactacg gaattgattc ttttaccctg cgatatgcca tttgtctgtt     7380

gggatctttt cctcttaacg ctattctgaa gcggattcct gaaaagcgaa tcggcctgaa     7440

gtgttgtttt atcatttcta tgtccatgtt ttatctcttc ggcgttctga atctcgtgag     7500

cggatttcga accctcttca tttccacaat gttcacatac cttatctctc ggttctaccg     7560

atccaagttt atgccccatc tcaacttcat gttcgtcatg ggccacttgg ctatcaacca     7620

cattcatgct cagttcctga acgaacaaac tcaaacgacc gtcgatatta catcctcgca     7680

gatggtcctg gctatgaagc tgacaagctt tgcctggtct tactatgacg gttcgtgtac     7740

gagcgagtcc gacttcaagg accttaccga acaccagaag tcccgagccg tccgaggcca     7800

tcctcccctt ctgaaatttt tggcttacgc ctttttctac tctacccttc tcaccggtcc     7860

ctccttcgat tacgctgatt tcgactcttg gctgaactgc gaaatgttcc gggaccttcc     7920

cgagtccaag aaacccatgc gaagacatca tcctggtgag cggcgtcaga ttcccaagaa     7980

cggcaagctc gccctgtgga aggttgtcca gggcctcgcc tggatgattc tgagcacgtt     8040

gggtatgaag cacttccccg tgaagtacgt gctggacaag gacggatttc ctacccgttc     8100

ctttatcttc cgtattcatt atctgtttct gctgggattc atccaccgat ttaagtatta     8160

cgctgcgtgg acgattagcg aaggttcgtg cattctctgt ggtcttggtt ataatggata     8220

cgattctaag acccagaaga tccggtggga tcgagtgcgg aatattgata tttggacagt     8280

ggagactgca caaaacaccc gagagatgct ggaagcgtgg aacatgaata ctaacaaatg     8340

gctgaagtat agcgtgtatc ttagagtgac taagaagggt aagaagccag gttttcgatc     8400

taccctgttt accttcctga cctccgcctt ttggcacggt acccgtcctg gatactacct     8460

taccttcgca actggtgccc tgtaccaaac ctgtggaaag atctatagac gaaactttcg     8520

tcccatcttt ctgagagaag atggcgtgac acctctcccg tccaagaaga tttacgacct     8580

ggtcggcatt tacgctatta agctggcctt tggttacatg gttcaaccct tcattatcct     8640

tgacctgaag ccctctctta tggtttgggg atccgtgtat ttctacgtgc atattattgt     8700

ggccttctcg ttctttctgt tccgaggacc atacgctaag caggttactg aatttttcaa     8760

aagcaagcaa ccgaaggaga tcttcatccg aaagcagaag aagttggaaa aagacatctc     8820

tgcctcttcc cccaacctcg gaggtattct taaggcaaaa atcgaacatg agaagggaaa     8880

gacggcagag gaggaagaga tgaacttggg cattccaccc atcgaactgg agaagtggga     8940

caacgccaag gaggactggg aggatttctg caaggactac aaggagtggc ggaacaagaa     9000

cggactggaa attgaagagg agaacctgtc caaggccttc gagcgattta agcaggaatt     9060

ttccaacgct gcgtcgggct ctggtgaacg ggttcggaaa atgtccttct ccggatattc     9120

tcctaaaccc atctcgaaga aagaagaata ggcggccgca tgagaagata aatatataaa     9180

tacattgaga tattaaatgc gctagattag agagcctcat actgctcgga gagaagccaa     9240

gacgagtact caaaggggat tacaccatcc atatccacag acacaagctg gggaaaggtt     9300

ctatatacac tttccggaat accgtagttt ccgatgttat caatgggggc agccaggatt     9360

tcaggcactt cggtgtctcg gggtgaaatg gcgttcttgg cctccatcaa gtcgtaccat     9420

gtcttcattt gcctgtcaaa gtaaaacaga agcagatgaa gaatgaactt gaagtgaagg     9480

aatttaaatg taacgaaact gaaatttgac cagatattgt gtccgcggtg gagctccagc     9540

ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt     9600

cctgtgtgaa attgttatcc gctcacaagc ttccacacaa c                         9641


<210>  140
<211>  34
<212>  DNA
<213>  Escherichia coli

<400>  140
ataacttcgt ataatgtatg ctatacgaag ttat                                   34


<210>  141
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 798

<400>  141
ctaattcaca accatggcct ttccatgggc agataagtgg                             40


<210>  142
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 799

<400>  142
ctcatgcggc cgcttacttg gtcttgatgg tgtcct                                 36


<210>  143
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 800

<400>  143
cgatagttag tagacaacaa tcgatagttg gagcaaggga                             40


<210>  144
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 801

<400>  144
gaaaggccat ggttgtgaat tagggtggtg agaatg                                 36


<210>  145
<211>  9320
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY168

<400>  145
gtacgataac ttcgtatagc atacattata cgaagttatc gcgtcgacga gtatctgtct       60

gactcgtcat tgccgccttt ggagtacgac tccaactatg agtgtgcttg gatcactttg      120

acgatacatt cttcgttgga ggctgtgggt ctgacagctg cgttttcggc gcggttggcc      180

gacaacaata tcagctgcaa cgtcattgct ggctttcatc atgatcacat ttttgtcggc      240

aaaggcgacg cccagagagc cattgacgtt ctttctaatt tggaccgata gccgtatagt      300

ccagtctatc tataagttca actaactcgt aactattacc ataacatata cttcactgcc      360

ccagataagg ttccgataaa aagttctgca gactaaattt atttcagtct cctcttcacc      420

accaaaatgc cctcctacga agctcgagct aacgtccaca agtccgcctt tgccgctcga      480

gtgctcaagc tcgtggcagc caagaaaacc aacctgtgtg cttctctgga tgttaccacc      540

accaaggagc tcattgagct tgccgataag gtcggacctt atgtgtgcat gatcaaaacc      600

catatcgaca tcattgacga cttcacctac gccggcactg tgctccccct caaggaactt      660

gctcttaagc acggtttctt cctgttcgag gacagaaagt tcgcagatat tggcaacact      720

gtcaagcacc agtaccggtg tcaccgaatc gccgagtggt ccgatatcac caacgcccac      780

ggtgtacccg gaaccggaat cattgctggc ctgcgagctg gtgccgagga aactgtctct      840

gaacagaaga aggaggacgt ctctgactac gagaactccc agtacaagga gttcctagtc      900

ccctctccca acgagaagct ggccagaggt ctgctcatgc tggccgagct gtcttgcaag      960

ggctctctgg ccactggcga gtactccaag cagaccattg agcttgcccg atccgacccc     1020

gagtttgtgg ttggcttcat tgcccagaac cgacctaagg gcgactctga ggactggctt     1080

attctgaccc ccggggtggg tcttgacgac aagggagacg ctctcggaca gcagtaccga     1140

actgttgagg atgtcatgtc taccggaacg gatatcataa ttgtcggccg aggtctgtac     1200

ggccagaacc gagatcctat tgaggaggcc aagcgatacc agaaggctgg ctgggaggct     1260

taccagaaga ttaactgtta gaggttagac tatggatatg taatttaact gtgtatatag     1320

agagcgtgca agtatggagc gcttgttcag cttgtatgat ggtcagacga cctgtctgat     1380

cgagtatgta tgatactgca caacctgtgt atccgcatga tctgtccaat ggggcatgtt     1440

gttgtgtttc tcgatacgga gatgctgggt acagtgctaa tacgttgaac tacttatact     1500

tatatgaggc tcgaagaaag ctgacttgtg tatgacttat tctcaactac atccccagtc     1560

acaataccac cactgcacta ccactacacc aaaaccatga tcaaaccacc catggacttc     1620

ctggaggcag aagaacttgt tatggaaaag ctcaagagag agatcataac ttcgtatagc     1680

atacattata cgaagttatc ctgcaggtaa aggaattcag gagagaccgg gttggcggcg     1740

tatttgtgtc ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc     1800

gggcccaacc ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac     1860

acttgccgtt aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta     1920

ctagtttctt tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa     1980

atttttttgc tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat     2040

tacctttcct cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt     2100

tccttctgag tataagaatc attcaccatg gacttcctgg aggcagaaga acttgttatg     2160

gaaaagctca agagagagaa gccaagatac tatcaagaca tgtgtcgcaa cttaattaag     2220

atgacgacat ttgcgagctg gacgaggaat agatggagcg tgtgttctga gtcgatgttt     2280

tctatggagt tgtgagtgtt agtagacatg atgggtttat atatgatgaa tgaatagatg     2340

tgattttgat ttgcacgatg gaattgagaa ctttgtaaac gtacatggga atgtatgaat     2400

gtgggggttt tgtgactgga taactgacgg tcagtggacg ccgttgttca aatatccaag     2460

agatgcgaga aactttgggt caagtgaaca tgtcctctct gttcaagtaa accatcaact     2520

atgggtagta tatttagtaa ggacaagagt tgagattctt tggagtccta gaaacgtatt     2580

ttcgcgttcc aagatcaaat tagtagagta atacgggcac gggaatccat tcatagtctc     2640

aattttccca taggtgtgct acaaggtgtt gagatgtggt acagtaccac catgattcga     2700

ggtaaagagc ccagaagtca ttgatgaggt caagaaatac acagatctac agctcaatac     2760

aatgaatatc ttctttcata ttcttcaggt gacaccaagg gtgtctattt tccccagaaa     2820

tgcgtgaaaa ggcgcgtgtg tagcgtggag tatgggttcg gttggcgtat ccttcatata     2880

tcgacgaaat agtagggcaa gagatgacaa aaagtatcta tatgtagaca gcgtagaata     2940

tggatttgat tggtataaat tcatttattg cgtgtctcac aaatactctc gataagttgg     3000

ggttaaactg gagatggaac aatgtcgata tctcgacgca tgcgacgtcg ggcccaattc     3060

gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga     3120

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg     3180

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga     3240

atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     3300

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     3360

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     3420

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     3480

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     3540

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     3600

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     3660

tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc     3720

ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc     3780

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     3840

taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc     3900

cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa     3960

acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa     4020

ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg     4080

atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa     4140

gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc     4200

acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc     4260

atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta     4320

accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag     4380

ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca     4440

acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata     4500

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     4560

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     4620

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     4680

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     4740

taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa     4800

tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt     4860

gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat     4920

cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg     4980

gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga     5040

gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac     5100

tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt     5160

ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag     5220

cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc     5280

gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag     5340

gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca     5400

gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt     5460

cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc     5520

tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc     5580

cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc     5640

cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa     5700

ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcgcgccac caatcacaat     5760

tctgaaaagc acatcttgat ctcctcattg cggggagtcc aacggtggtc ttattccccc     5820

gaatttcccg ctcaatctcg ttccagaccg acccggacac agtgcttaac gccgttccga     5880

aactctaccg cagatatgct ccaacggact gggctgcata gatgtgatcc tcggcttgga     5940

gaaatggata aaagccggcc aaaaaaaaag cggaaaaaag cggaaaaaaa gagaaaaaaa     6000

atcgcaaaat ttgaaaaata gggggaaaag acgcaaaaac gcaaggaggg gggagtatat     6060

gacactgata agcaagctca caacggttcc tcttattttt ttcctcatct tctgcctagg     6120

ttcccaaaat cccagatgct tctctccagt gccaaaagta agtaccccac aggttttcgg     6180

ccgaaaattc cacgtgcagc aacgtcgtgt ggggtgttaa aatgtggggg gggggaacca     6240

ggacaagagg ctcttgtggg agccgaatga gagcacaaag cgggcgggtg tgataagggc     6300

atttttgccc attttccctt ctcctgtctc tccgacggtg atggcgttgt gcgtcctcta     6360

tttcttttta tttctttttg ttttatttct ctgactaccg atttggtttg atttcctcaa     6420

ccccacacaa ataagctcgg gccgaggaat atatatatac acggacacag tcgccctgtg     6480

gacaacacgt cactacctct acgatacaca ccgtacgata gttagtagac aacaatcgat     6540

agttggagca agggagaaat gtagagtgtg aaagactcac tatggtccgg gcttatctcg     6600

accaatagcc aaagtctgga gtttctgaga gaaaaaggca agatacgtat gtaacaaagc     6660

gacgcatggt acaataatac cggaggcatg tatcatagag agttagtggt tcgatgatgg     6720

cactggtgcc tggtatgact ttatacggct gactacatat ttgtcctcag acatacaatt     6780

acagtcaagc acttaccctt ggacatctgt aggtaccccc cggccaagac gatctcagcg     6840

tgtcgtatgt cggattggcg tagctccctc gctcgtcaat tggctcccat ctactttctt     6900

ctgcttggct acacccagca tgtctgctat ggctcgtttt cgtgccttat ctatcctccc     6960

agtattacca actctaaatg acatgatgtg attgggtcta cactttcata tcagagataa     7020

ggagtagcac agttgcataa aaagcccaac tctaatcagc ttcttccttt cttgtaatta     7080

gtacaaaggt gattagcgaa atctggaagc ttagttggcc ctaaaaaaat caaaaaaagc     7140

aaaaaacgaa aaacgaaaaa ccacagtttt gagaacaggg aggtaacgaa ggatcgtata     7200

tatatatata tatatatata cccacggatc ccgagaccgg cctttgattc ttccctacaa     7260

ccaaccattc tcaccaccct aattcacaac catggccttt ccctgggcag ataagtgggc     7320

agccgatgcg tctgcatcta cagggctgcc tccggacctc ctcaagattg cattcactct     7380

ggtcatgtct tatccgctga gttctctcat gaaacggctg ccagatgacg ccaaaaacct     7440

caagatcatc tatatcatct ccgtgtccat cttctacatg gtgggtgtct tctccctcta     7500

tggcggagct gccactctgc tcttctcctc aatgggtacc ttcttcatca cccaatggaa     7560

gagcccttac atgccctggg tcaattttgg ttttgtcatg acccatctct tcgtcaatca     7620

cctgcgttcg cagtttttcc ccgaaacata cgaccccaat gtcattgaca tcaccggagc     7680

acagatggtt ctgtgtatga agctatcgtc ttttggatgg aacgtctacg atggatggca     7740

gattgagaag ggtgagcagc tcagcgagtt ccagactaaa agggctgttc tcaagcaccc     7800

cagtcttatg gacttcctag cttttgtgtt ctacttccct tccattctga caggtccttc     7860

ttacgactat atggagttcc ataactggct cgatctcagc ctgttcaagg agctggagaa     7920

agataaggac cccaagcgag ctgctcgacg aaagcgacac aagatccccc gatctggaat     7980

cgctgcttcc aagaaactcg ccgctggtat cttctggatc gttctgtgga cccaggtgga     8040

ctctcgaatc tccaccgcct acgcttactc agacgcattc accaaggagc acaacatctt     8100

tggacgaatt gtgtacctct acatgctcgg tttcatgtac cgactcaagt actacggagc     8160

ctggtccatt tccgagggag cctgcatctt gtctggcctc ggattccatg gcgtggaccc     8220

caaaactggc aagtacaagt gggaccgtgt ccagaacgtg gacccgtggg gattcgaaac     8280

tggtcaaaac acaaaggctc tgctggaggc ctggaaccag aacactaaca agtggctacg     8340

aaactatgtg tacctccgag tggtgcccaa aggccaaaag cctggattcc gagccactat     8400

cttcacattt gtggtttccg ccttctggca tggaactcga cctggctact atctcacctt     8460

tgtgaccgct gccatgtacc agtctgttgg taagttcttc cgacgatacc tgcgaccctt     8520

cttcatggag tctgatggaa agactgccgg tccctataag atctactacg acattgtgtg     8580

ttggatcgtt gtccaaaccg catttggata cgctacccag tcctttatga ttctagactt     8640

ctggctgtcg ctcaagtgtt ggaagaactc ctggttcctg taccacattg ctctgggcgc     8700

catctttgca atttctagcc cctacaaggc atgggcgatt cccaagatca agaaaaagca     8760

ggctggagcc gtcactgaca agaaggacgc caaggaggag gtgaagaagg acaccatcaa     8820

gaccaagtaa gcggccgcat gagaagataa atatataaat acattgagat attaaatgcg     8880

ctagattaga gagcctcata ctgctcggag agaagccaag acgagtactc aaaggggatt     8940

acaccatcca tatccacaga cacaagctgg ggaaaggttc tatatacact ttccggaata     9000

ccgtagtttc cgatgttatc aatgggggca gccaggattt caggcacttc ggtgtctcgg     9060

ggtgaaatgg cgttcttggc ctccatcaag tcgtaccatg tcttcatttg cctgtcaaag     9120

taaaacagaa gcagatgaag aatgaacttg aagtgaagga atttaaatgt aacgaaactg     9180

aaatttgacc agatattgtg tccgcggtgg agctccagct tttgttccct ttagtgaggg     9240

ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg     9300

ctcacaagct tccacacaac                                                 9320


<210>  146
<211>  8726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY208

<400>  146
gtacgataac ttcgtatagc atacattata cgaagttatc gcgtcgacga gtatctgtct       60

gactcgtcat tgccgccttt ggagtacgac tccaactatg agtgtgcttg gatcactttg      120

acgatacatt cttcgttgga ggctgtgggt ctgacagctg cgttttcggc gcggttggcc      180

gacaacaata tcagctgcaa cgtcattgct ggctttcatc atgatcacat ttttgtcggc      240

aaaggcgacg cccagagagc cattgacgtt ctttctaatt tggaccgata gccgtatagt      300

ccagtctatc tataagttca actaactcgt aactattacc ataacatata cttcactgcc      360

ccagataagg ttccgataaa aagttctgca gactaaattt atttcagtct cctcttcacc      420

accaaaatgc cctcctacga agctcgagct aacgtccaca agtccgcctt tgccgctcga      480

gtgctcaagc tcgtggcagc caagaaaacc aacctgtgtg cttctctgga tgttaccacc      540

accaaggagc tcattgagct tgccgataag gtcggacctt atgtgtgcat gatcaaaacc      600

catatcgaca tcattgacga cttcacctac gccggcactg tgctccccct caaggaactt      660

gctcttaagc acggtttctt cctgttcgag gacagaaagt tcgcagatat tggcaacact      720

gtcaagcacc agtaccggtg tcaccgaatc gccgagtggt ccgatatcac caacgcccac      780

ggtgtacccg gaaccggaat cattgctggc ctgcgagctg gtgccgagga aactgtctct      840

gaacagaaga aggaggacgt ctctgactac gagaactccc agtacaagga gttcctagtc      900

ccctctccca acgagaagct ggccagaggt ctgctcatgc tggccgagct gtcttgcaag      960

ggctctctgg ccactggcga gtactccaag cagaccattg agcttgcccg atccgacccc     1020

gagtttgtgg ttggcttcat tgcccagaac cgacctaagg gcgactctga ggactggctt     1080

attctgaccc ccggggtggg tcttgacgac aagggagacg ctctcggaca gcagtaccga     1140

actgttgagg atgtcatgtc taccggaacg gatatcataa ttgtcggccg aggtctgtac     1200

ggccagaacc gagatcctat tgaggaggcc aagcgatacc agaaggctgg ctgggaggct     1260

taccagaaga ttaactgtta gaggttagac tatggatatg taatttaact gtgtatatag     1320

agagcgtgca agtatggagc gcttgttcag cttgtatgat ggtcagacga cctgtctgat     1380

cgagtatgta tgatactgca caacctgtgt atccgcatga tctgtccaat ggggcatgtt     1440

gttgtgtttc tcgatacgga gatgctgggt acagtgctaa tacgttgaac tacttatact     1500

tatatgaggc tcgaagaaag ctgacttgtg tatgacttat tctcaactac atccccagtc     1560

acaataccac cactgcacta ccactacacc aaaaccatga tcaaaccacc catggacttc     1620

ctggaggcag aagaacttgt tatggaaaag ctcaagagag agatcataac ttcgtatagc     1680

atacattata cgaagttatc ctgcaggtaa aggaattcag gagagaccgg gttggcggcg     1740

tatttgtgtc ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc     1800

gggcccaacc ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac     1860

acttgccgtt aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta     1920

ctagtttctt tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa     1980

atttttttgc tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat     2040

tacctttcct cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt     2100

tccttctgag tataagaatc attcaccatg gacttcctgg aggcagaaga acttgttatg     2160

gaaaagctca agagagagaa gccaagatac tatcaagaca tgtgtcgcaa cttaattaag     2220

atgacgacat ttgcgagctg gacgaggaat agatggagcg tgtgttctga gtcgatgttt     2280

tctatggagt tgtgagtgtt agtagacatg atgggtttat atatgatgaa tgaatagatg     2340

tgattttgat ttgcacgatg gaattgagaa ctttgtaaac gtacatggga atgtatgaat     2400

gtgggggttt tgtgactgga taactgacgg tcagtggacg ccgttgttca aatatccaag     2460

agatgcgaga aactttgggt caagtgaaca tgtcctctct gttcaagtaa accatcaact     2520

atgggtagta tatttagtaa ggacaagagt tgagattctt tggagtccta gaaacgtatt     2580

ttcgcgttcc aagatcaaat tagtagagta atacgggcac gggaatccat tcatagtctc     2640

aattttccca taggtgtgct acaaggtgtt gagatgtggt acagtaccac catgattcga     2700

ggtaaagagc ccagaagtca ttgatgaggt caagaaatac acagatctac agctcaatac     2760

aatgaatatc ttctttcata ttcttcaggt gacaccaagg gtgtctattt tccccagaaa     2820

tgcgtgaaaa ggcgcgtgtg tagcgtggag tatgggttcg gttggcgtat ccttcatata     2880

tcgacgaaat agtagggcaa gagatgacaa aaagtatcta tatgtagaca gcgtagaata     2940

tggatttgat tggtataaat tcatttattg cgtgtctcac aaatactctc gataagttgg     3000

ggttaaactg gagatggaac aatgtcgata tctcgacgca tgcgacgtcg ggcccaattc     3060

gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga     3120

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg     3180

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga     3240

atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     3300

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     3360

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     3420

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     3480

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     3540

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     3600

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     3660

tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc     3720

ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc     3780

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     3840

taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc     3900

cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa     3960

acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa     4020

ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg     4080

atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa     4140

gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc     4200

acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc     4260

atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta     4320

accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag     4380

ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca     4440

acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata     4500

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     4560

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     4620

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     4680

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     4740

taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa     4800

tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt     4860

gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat     4920

cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg     4980

gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga     5040

gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac     5100

tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt     5160

ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag     5220

cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc     5280

gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag     5340

gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca     5400

gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt     5460

cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc     5520

tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc     5580

cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc     5640

cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa     5700

ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcgcgccac caatcacaat     5760

tctgaaaagc acatcttgat ctcctcattg cggggagtcc aacggtggtc ttattccccc     5820

gaatttcccg ctcaatctcg ttccagaccg acccggacac agtgcttaac gccgttccga     5880

aactctaccg cagatatgct ccaacggact gggctgcata gatgtgatcc tcggcttgga     5940

gaaatggata aaagccggcc aaaaaaaaag cggaaaaaag cggaaaaaaa gagaaaaaaa     6000

atcgcaaaat ttgaaaaata gggggaaaag acgcaaaaac gcaaggaggg gggagtatat     6060

gacactgata agcaagctca caacggttcc tcttattttt ttcctcatct tctgcctagg     6120

ttcccaaaat cccagatgct tctctccagt gccaaaagta agtaccccac aggttttcgg     6180

ccgaaaattc cacgtgcagc aacgtcgtgt ggggtgttaa aatgtggggg gggggaacca     6240

ggacaagagg ctcttgtggg agccgaatga gagcacaaag cgggcgggtg tgataagggc     6300

atttttgccc attttccctt ctcctgtctc tccgacggtg atggcgttgt gcgtcctcta     6360

tttcttttta tttctttttg ttttatttct ctgactaccg atttggtttg atttcctcaa     6420

ccccacacaa ataagctcgg gccgaggaat atatatatac acggacacag tcgccctgtg     6480

gacaacacgt cactacctct acgatacaca ccgtacgata gttagtagac aacaatcgat     6540

agttggagca agggagaaat gtagagtgtg aaagactcac tatggtccgg gcttatctcg     6600

accaatagcc aaagtctgga gtttctgaga gaaaaaggca agatacgtat gtaacaaagc     6660

gacgcatggt acaataatac cggaggcatg tatcatagag agttagtggt tcgatgatgg     6720

cactggtgcc tggtatgact ttatacggct gactacatat ttgtcctcag acatacaatt     6780

acagtcaagc acttaccctt ggacatctgt aggtaccccc cggccaagac gatctcagcg     6840

tgtcgtatgt cggattggcg tagctccctc gctcgtcaat tggctcccat ctactttctt     6900

ctgcttggct acacccagca tgtctgctat ggctcgtttt cgtgccttat ctatcctccc     6960

agtattacca actctaaatg acatgatgtg attgggtcta cactttcata tcagagataa     7020

ggagtagcac agttgcataa aaagcccaac tctaatcagc ttcttccttt cttgtaatta     7080

gtacaaaggt gattagcgaa atctggaagc ttagttggcc ctaaaaaaat caaaaaaagc     7140

aaaaaacgaa aaacgaaaaa ccacagtttt gagaacaggg aggtaacgaa ggatcgtata     7200

tatatatata tatatatata cccacggatc ccgagaccgg cctttgattc ttccctacaa     7260

ccaaccattc tcaccaccct aattcacaac catgtctatt ggttcgtcca accccgtgct     7320

cttggctgcg attcccttcg tctacctgtt tgtcctccca cgagtcctgg ctttcctgcc     7380

tcagaaggct cagttcctgg ccaaatgtat tgtggtcctg attgccacgc ttatcatgtc     7440

cgttgcaggc tgcttcatct cgatcgtgtg cgctcttctg gacaagagat acgtcatcaa     7500

ttacgttgtg tcgcgattgt tctccttcct tgccgctcga ccgtgtggtg tgacctataa     7560

gattgttggt gaggaacacc tcgataagta ccctgctatc gtggtctgta accatcaatc     7620

ctctatggat atgatggttt tgggacgagt ttttccaaag cactgcgttg tcatggcgaa     7680

gaaggaactc ctgtactttc cctttttggg aatgtttatg aaactgagca acgctatctt     7740

catcgaccgg aagaaccaca agaaagccat cgagtctacc acccaagccg tggcggacat     7800

gaagaagcac aactctggaa tctggatttt cccagagggc acccggtcta gactggacaa     7860

ggcagacctg ctgcccttca agaaaggtgc ctttcatctt gcaattcagg cccagctccc     7920

tattctcccc attatctcgc agggctattc ccatatctac gactcttcga agcggtactt     7980

ccccggtgga gagctcgaga tcagagtcct ggagcccatt cctacaactg gcctcactac     8040

tgatgatgtg aacgacctga tggacaagac acgaaacctt atgctcaagc acttgaagga     8100

gatggattcc cagtattcgt cgagcactgc tgaaaatgga tccacgcaca tcgacgccga     8160

tattgccaag tctacagcca ccagcattgg caacactgac gacgcaatta caaaacgtcg     8220

tacccctaag gaataagcgg ccgcatgaga agataaatat ataaatacat tgagatatta     8280

aatgcgctag attagagagc ctcatactgc tcggagagaa gccaagacga gtactcaaag     8340

gggattacac catccatatc cacagacaca agctggggaa aggttctata tacactttcc     8400

ggaataccgt agtttccgat gttatcaatg ggggcagcca ggatttcagg cacttcggtg     8460

tctcggggtg aaatggcgtt cttggcctcc atcaagtcgt accatgtctt catttgcctg     8520

tcaaagtaaa acagaagcag atgaagaatg aacttgaagt gaaggaattt aaatgtaacg     8580

aaactgaaat ttgaccagat attgtgtccg cggtggagct ccagcttttg ttccctttag     8640

tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt     8700

tatccgctca caagcttcca cacaac                                          8726


<210>  147
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 856

<400>  147
tcacaacaca tgtccgttgc atccaagctc g                                      31


<210>  148
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 857

<400>  148
tttagcggcc gcctactgag tcttctgg                                          28


<210>  149
<211>  8630
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY207

<400>  149
gtacgataac ttcgtatagc atacattata cgaagttatc gcgtcgacga gtatctgtct       60

gactcgtcat tgccgccttt ggagtacgac tccaactatg agtgtgcttg gatcactttg      120

acgatacatt cttcgttgga ggctgtgggt ctgacagctg cgttttcggc gcggttggcc      180

gacaacaata tcagctgcaa cgtcattgct ggctttcatc atgatcacat ttttgtcggc      240

aaaggcgacg cccagagagc cattgacgtt ctttctaatt tggaccgata gccgtatagt      300

ccagtctatc tataagttca actaactcgt aactattacc ataacatata cttcactgcc      360

ccagataagg ttccgataaa aagttctgca gactaaattt atttcagtct cctcttcacc      420

accaaaatgc cctcctacga agctcgagct aacgtccaca agtccgcctt tgccgctcga      480

gtgctcaagc tcgtggcagc caagaaaacc aacctgtgtg cttctctgga tgttaccacc      540

accaaggagc tcattgagct tgccgataag gtcggacctt atgtgtgcat gatcaaaacc      600

catatcgaca tcattgacga cttcacctac gccggcactg tgctccccct caaggaactt      660

gctcttaagc acggtttctt cctgttcgag gacagaaagt tcgcagatat tggcaacact      720

gtcaagcacc agtaccggtg tcaccgaatc gccgagtggt ccgatatcac caacgcccac      780

ggtgtacccg gaaccggaat cattgctggc ctgcgagctg gtgccgagga aactgtctct      840

gaacagaaga aggaggacgt ctctgactac gagaactccc agtacaagga gttcctagtc      900

ccctctccca acgagaagct ggccagaggt ctgctcatgc tggccgagct gtcttgcaag      960

ggctctctgg ccactggcga gtactccaag cagaccattg agcttgcccg atccgacccc     1020

gagtttgtgg ttggcttcat tgcccagaac cgacctaagg gcgactctga ggactggctt     1080

attctgaccc ccggggtggg tcttgacgac aagggagacg ctctcggaca gcagtaccga     1140

actgttgagg atgtcatgtc taccggaacg gatatcataa ttgtcggccg aggtctgtac     1200

ggccagaacc gagatcctat tgaggaggcc aagcgatacc agaaggctgg ctgggaggct     1260

taccagaaga ttaactgtta gaggttagac tatggatatg taatttaact gtgtatatag     1320

agagcgtgca agtatggagc gcttgttcag cttgtatgat ggtcagacga cctgtctgat     1380

cgagtatgta tgatactgca caacctgtgt atccgcatga tctgtccaat ggggcatgtt     1440

gttgtgtttc tcgatacgga gatgctgggt acagtgctaa tacgttgaac tacttatact     1500

tatatgaggc tcgaagaaag ctgacttgtg tatgacttat tctcaactac atccccagtc     1560

acaataccac cactgcacta ccactacacc aaaaccatga tcaaaccacc catggacttc     1620

ctggaggcag aagaacttgt tatggaaaag ctcaagagag agatcataac ttcgtatagc     1680

atacattata cgaagttatc ctgcaggtaa aggaattcag gagagaccgg gttggcggcg     1740

tatttgtgtc ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc     1800

gggcccaacc ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac     1860

acttgccgtt aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta     1920

ctagtttctt tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa     1980

atttttttgc tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat     2040

tacctttcct cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt     2100

tccttctgag tataagaatc attcaccatg gacttcctgg aggcagaaga acttgttatg     2160

gaaaagctca agagagagaa gccaagatac tatcaagaca tgtgtcgcaa cttaattaag     2220

atgacgacat ttgcgagctg gacgaggaat agatggagcg tgtgttctga gtcgatgttt     2280

tctatggagt tgtgagtgtt agtagacatg atgggtttat atatgatgaa tgaatagatg     2340

tgattttgat ttgcacgatg gaattgagaa ctttgtaaac gtacatggga atgtatgaat     2400

gtgggggttt tgtgactgga taactgacgg tcagtggacg ccgttgttca aatatccaag     2460

agatgcgaga aactttgggt caagtgaaca tgtcctctct gttcaagtaa accatcaact     2520

atgggtagta tatttagtaa ggacaagagt tgagattctt tggagtccta gaaacgtatt     2580

ttcgcgttcc aagatcaaat tagtagagta atacgggcac gggaatccat tcatagtctc     2640

aattttccca taggtgtgct acaaggtgtt gagatgtggt acagtaccac catgattcga     2700

ggtaaagagc ccagaagtca ttgatgaggt caagaaatac acagatctac agctcaatac     2760

aatgaatatc ttctttcata ttcttcaggt gacaccaagg gtgtctattt tccccagaaa     2820

tgcgtgaaaa ggcgcgtgtg tagcgtggag tatgggttcg gttggcgtat ccttcatata     2880

tcgacgaaat agtagggcaa gagatgacaa aaagtatcta tatgtagaca gcgtagaata     2940

tggatttgat tggtataaat tcatttattg cgtgtctcac aaatactctc gataagttgg     3000

ggttaaactg gagatggaac aatgtcgata tctcgacgca tgcgacgtcg ggcccaattc     3060

gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga     3120

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg     3180

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga     3240

atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     3300

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     3360

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     3420

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     3480

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     3540

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     3600

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     3660

tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc     3720

ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc     3780

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     3840

taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc     3900

cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa     3960

acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa     4020

ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg     4080

atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa     4140

gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc     4200

acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc     4260

atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta     4320

accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag     4380

ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca     4440

acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata     4500

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     4560

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     4620

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     4680

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     4740

taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa     4800

tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt     4860

gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat     4920

cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg     4980

gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga     5040

gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac     5100

tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt     5160

ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag     5220

cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc     5280

gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag     5340

gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca     5400

gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt     5460

cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc     5520

tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc     5580

cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc     5640

cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa     5700

ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcgcgccac caatcacaat     5760

tctgaaaagc acatcttgat ctcctcattg cggggagtcc aacggtggtc ttattccccc     5820

gaatttcccg ctcaatctcg ttccagaccg acccggacac agtgcttaac gccgttccga     5880

aactctaccg cagatatgct ccaacggact gggctgcata gatgtgatcc tcggcttgga     5940

gaaatggata aaagccggcc aaaaaaaaag cggaaaaaag cggaaaaaaa gagaaaaaaa     6000

atcgcaaaat ttgaaaaata gggggaaaag acgcaaaaac gcaaggaggg gggagtatat     6060

gacactgata agcaagctca caacggttcc tcttattttt ttcctcatct tctgcctagg     6120

ttcccaaaat cccagatgct tctctccagt gccaaaagta agtaccccac aggttttcgg     6180

ccgaaaattc cacgtgcagc aacgtcgtgt ggggtgttaa aatgtggggg gggggaacca     6240

ggacaagagg ctcttgtggg agccgaatga gagcacaaag cgggcgggtg tgataagggc     6300

atttttgccc attttccctt ctcctgtctc tccgacggtg atggcgttgt gcgtcctcta     6360

tttcttttta tttctttttg ttttatttct ctgactaccg atttggtttg atttcctcaa     6420

ccccacacaa ataagctcgg gccgaggaat atatatatac acggacacag tcgccctgtg     6480

gacaacacgt cactacctct acgatacaca ccgtacgata gttagtagac aacaatcgat     6540

agttggagca agggagaaat gtagagtgtg aaagactcac tatggtccgg gcttatctcg     6600

accaatagcc aaagtctgga gtttctgaga gaaaaaggca agatacgtat gtaacaaagc     6660

gacgcatggt acaataatac cggaggcatg tatcatagag agttagtggt tcgatgatgg     6720

cactggtgcc tggtatgact ttatacggct gactacatat ttgtcctcag acatacaatt     6780

acagtcaagc acttaccctt ggacatctgt aggtaccccc cggccaagac gatctcagcg     6840

tgtcgtatgt cggattggcg tagctccctc gctcgtcaat tggctcccat ctactttctt     6900

ctgcttggct acacccagca tgtctgctat ggctcgtttt cgtgccttat ctatcctccc     6960

agtattacca actctaaatg acatgatgtg attgggtcta cactttcata tcagagataa     7020

ggagtagcac agttgcataa aaagcccaac tctaatcagc ttcttccttt cttgtaatta     7080

gtacaaaggt gattagcgaa atctggaagc ttagttggcc ctaaaaaaat caaaaaaagc     7140

aaaaaacgaa aaacgaaaaa ccacagtttt gagaacaggg aggtaacgaa ggatcgtata     7200

tatatatata tatatatata cccacggatc ccgagaccgg cctttgattc ttccctacaa     7260

ccaaccattc tcaccaccct aattcacaac catgtccgtt gcatccaagc tcgtcttcta     7320

cgtccgcgcc gccatcgccg tggtcatctt tgccgcctgt gccacctacg gcgtgctggc     7380

gtccaccatt ctcaccgcca tcggcaagca gggcctggcc caatggaccg ttgccagagc     7440

cttctactac tcggtgcgca tcttcctggg tatcagcatc aagctgcgta gccggcaggt     7500

gaccggaacc gccggtctgg atgcctccaa gatccaggtc gccaacacca ccaagcccat     7560

tgacgacatc accaaacacc tgccccgacc atgcattctg atttccaacc accagaacga     7620

aatggacatt ctggtgctcg gtcgcatctt cccccagtac tgctccgtca ccgccaaaaa     7680

ggccctcaag tggtaccctc tgctgggcca gttcatggcg ctgtccggca ccatcttcct     7740

ggaccgaaag gaccgaacca agtccgtgca gaccctcggc ggcgccgtca agaccatcca     7800

gagcggcaac ggaggcaagg gccagagcgt cttcatgttc cccgagggaa cccgatccta     7860

ctccaaggac gtcggcatca tgcccttcaa gaagggctgt ttccacctgg cggtccagtc     7920

gggcgctccc attgtccccg tggtggtcca gaacacctcc cgaatgtttt ctttcggccg     7980

aggcaagctg gacgccggag agatccttgt cgacgtcctg agccccattg agaccaaggg     8040

tctggacgcc agcaacgtcg acgctctcat ggccaccact tataaggcca tgtgcgagac     8100

tgccgaccag attggctacg ctggccagaa gactcagtag gcggccgcat gagaagataa     8160

atatataaat acattgagat attaaatgcg ctagattaga gagcctcata ctgctcggag     8220

agaagccaag acgagtactc aaaggggatt acaccatcca tatccacaga cacaagctgg     8280

ggaaaggttc tatatacact ttccggaata ccgtagtttc cgatgttatc aatgggggca     8340

gccaggattt caggcacttc ggtgtctcgg ggtgaaatgg cgttcttggc ctccatcaag     8400

tcgtaccatg tcttcatttg cctgtcaaag taaaacagaa gcagatgaag aatgaacttg     8460

aagtgaagga atttaaatgt aacgaaactg aaatttgacc agatattgtg tccgcggtgg     8520

agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca     8580

tagctgtttc ctgtgtgaaa ttgttatccg ctcacaagct tccacacaac                8630


<210>  150
<211>  8630
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY175

<400>  150
gtacgataac ttcgtatagc atacattata cgaagttatc gcgtcgacga gtatctgtct       60

gactcgtcat tgccgccttt ggagtacgac tccaactatg agtgtgcttg gatcactttg      120

acgatacatt cttcgttgga ggctgtgggt ctgacagctg cgttttcggc gcggttggcc      180

gacaacaata tcagctgcaa cgtcattgct ggctttcatc atgatcacat ttttgtcggc      240

aaaggcgacg cccagagagc cattgacgtt ctttctaatt tggaccgata gccgtatagt      300

ccagtctatc tataagttca actaactcgt aactattacc ataacatata cttcactgcc      360

ccagataagg ttccgataaa aagttctgca gactaaattt atttcagtct cctcttcacc      420

accaaaatgc cctcctacga agctcgagct aacgtccaca agtccgcctt tgccgctcga      480

gtgctcaagc tcgtggcagc caagaaaacc aacctgtgtg cttctctgga tgttaccacc      540

accaaggagc tcattgagct tgccgataag gtcggacctt atgtgtgcat gatcaaaacc      600

catatcgaca tcattgacga cttcacctac gccggcactg tgctccccct caaggaactt      660

gctcttaagc acggtttctt cctgttcgag gacagaaagt tcgcagatat tggcaacact      720

gtcaagcacc agtaccggtg tcaccgaatc gccgagtggt ccgatatcac caacgcccac      780

ggtgtacccg gaaccggaat cattgctggc ctgcgagctg gtgccgagga aactgtctct      840

gaacagaaga aggaggacgt ctctgactac gagaactccc agtacaagga gttcctagtc      900

ccctctccca acgagaagct ggccagaggt ctgctcatgc tggccgagct gtcttgcaag      960

ggctctctgg ccactggcga gtactccaag cagaccattg agcttgcccg atccgacccc     1020

gagtttgtgg ttggcttcat tgcccagaac cgacctaagg gcgactctga ggactggctt     1080

attctgaccc ccggggtggg tcttgacgac aagggagacg ctctcggaca gcagtaccga     1140

actgttgagg atgtcatgtc taccggaacg gatatcataa ttgtcggccg aggtctgtac     1200

ggccagaacc gagatcctat tgaggaggcc aagcgatacc agaaggctgg ctgggaggct     1260

taccagaaga ttaactgtta gaggttagac tatggatatg taatttaact gtgtatatag     1320

agagcgtgca agtatggagc gcttgttcag cttgtatgat ggtcagacga cctgtctgat     1380

cgagtatgta tgatactgca caacctgtgt atccgcatga tctgtccaat ggggcatgtt     1440

gttgtgtttc tcgatacgga gatgctgggt acagtgctaa tacgttgaac tacttatact     1500

tatatgaggc tcgaagaaag ctgacttgtg tatgacttat tctcaactac atccccagtc     1560

acaataccac cactgcacta ccactacacc aaaaccatga tcaaaccacc catggacttc     1620

ctggaggcag aagaacttgt tatggaaaag ctcaagagag agatcataac ttcgtatagc     1680

atacattata cgaagttatc ctgcaggtaa aggaattcag gagagaccgg gttggcggcg     1740

tatttgtgtc ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc     1800

gggcccaacc ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac     1860

acttgccgtt aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta     1920

ctagtttctt tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa     1980

atttttttgc tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat     2040

tacctttcct cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt     2100

tccttctgag tataagaatc attcaccatg gacttcctgg aggcagaaga acttgttatg     2160

gaaaagctca agagagagaa gccaagatac tatcaagaca tgtgtcgcaa cttaattaag     2220

atgacgacat ttgcgagctg gacgaggaat agatggagcg tgtgttctga gtcgatgttt     2280

tctatggagt tgtgagtgtt agtagacatg atgggtttat atatgatgaa tgaatagatg     2340

tgattttgat ttgcacgatg gaattgagaa ctttgtaaac gtacatggga atgtatgaat     2400

gtgggggttt tgtgactgga taactgacgg tcagtggacg ccgttgttca aatatccaag     2460

agatgcgaga aactttgggt caagtgaaca tgtcctctct gttcaagtaa accatcaact     2520

atgggtagta tatttagtaa ggacaagagt tgagattctt tggagtccta gaaacgtatt     2580

ttcgcgttcc aagatcaaat tagtagagta atacgggcac gggaatccat tcatagtctc     2640

aattttccca taggtgtgct acaaggtgtt gagatgtggt acagtaccac catgattcga     2700

ggtaaagagc ccagaagtca ttgatgaggt caagaaatac acagatctac agctcaatac     2760

aatgaatatc ttctttcata ttcttcaggt gacaccaagg gtgtctattt tccccagaaa     2820

tgcgtgaaaa ggcgcgtgtg tagcgtggag tatgggttcg gttggcgtat ccttcatata     2880

tcgacgaaat agtagggcaa gagatgacaa aaagtatcta tatgtagaca gcgtagaata     2940

tggatttgat tggtataaat tcatttattg cgtgtctcac aaatactctc gataagttgg     3000

ggttaaactg gagatggaac aatgtcgata tctcgacgca tgcgacgtcg ggcccaattc     3060

gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga     3120

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg     3180

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga     3240

atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     3300

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     3360

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     3420

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     3480

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     3540

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     3600

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     3660

tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc     3720

ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc     3780

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     3840

taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc     3900

cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa     3960

acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa     4020

ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg     4080

atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa     4140

gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc     4200

acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc     4260

atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta     4320

accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag     4380

ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca     4440

acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata     4500

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     4560

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     4620

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     4680

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     4740

taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa     4800

tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt     4860

gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat     4920

cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg     4980

gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga     5040

gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac     5100

tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt     5160

ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag     5220

cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc     5280

gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag     5340

gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca     5400

gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt     5460

cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc     5520

tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc     5580

cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc     5640

cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa     5700

ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcgcgccac caatcacaat     5760

tctgaaaagc acatcttgat ctcctcattg cggggagtcc aacggtggtc ttattccccc     5820

gaatttcccg ctcaatctcg ttccagaccg acccggacac agtgcttaac gccgttccga     5880

aactctaccg cagatatgct ccaacggact gggctgcata gatgtgatcc tcggcttgga     5940

gaaatggata aaagccggcc aaaaaaaaag cggaaaaaag cggaaaaaaa gagaaaaaaa     6000

atcgcaaaat ttgaaaaata gggggaaaag acgcaaaaac gcaaggaggg gggagtatat     6060

gacactgata agcaagctca caacggttcc tcttattttt ttcctcatct tctgcctagg     6120

ttcccaaaat cccagatgct tctctccagt gccaaaagta agtaccccac aggttttcgg     6180

ccgaaaattc cacgtgcagc aacgtcgtgt ggggtgttaa aatgtggggg gggggaacca     6240

ggacaagagg ctcttgtggg agccgaatga gagcacaaag cgggcgggtg tgataagggc     6300

atttttgccc attttccctt ctcctgtctc tccgacggtg atggcgttgt gcgtcctcta     6360

tttcttttta tttctttttg ttttatttct ctgactaccg atttggtttg atttcctcaa     6420

ccccacacaa ataagctcgg gccgaggaat atatatatac acggacacag tcgccctgtg     6480

gacaacacgt cactacctct acgatacaca ccgtacgata gttagtagac aacaatcgat     6540

agttggagca agggagaaat gtagagtgtg aaagactcac tatggtccgg gcttatctcg     6600

accaatagcc aaagtctgga gtttctgaga gaaaaaggca agatacgtat gtaacaaagc     6660

gacgcatggt acaataatac cggaggcatg tatcatagag agttagtggt tcgatgatgg     6720

cactggtgcc tggtatgact ttatacggct gactacatat ttgtcctcag acatacaatt     6780

acagtcaagc acttaccctt ggacatctgt aggtaccccc cggccaagac gatctcagcg     6840

tgtcgtatgt cggattggcg tagctccctc gctcgtcaat tggctcccat ctactttctt     6900

ctgcttggct acacccagca tgtctgctat ggctcgtttt cgtgccttat ctatcctccc     6960

agtattacca actctaaatg acatgatgtg attgggtcta cactttcata tcagagataa     7020

ggagtagcac agttgcataa aaagcccaac tctaatcagc ttcttccttt cttgtaatta     7080

gtacaaaggt gattagcgaa atctggaagc ttagttggcc ctaaaaaaat caaaaaaagc     7140

aaaaaacgaa aaacgaaaaa ccacagtttt gagaacaggg aggtaacgaa ggatcgtata     7200

tatatatata tatatatata cccacggatc ccgagaccgg cctttgattc ttccctacaa     7260

ccaaccattc tcaccaccct aattcacaac catggagaac ttctggtcca tcgtcgtgtt     7320

ctttctgctc tccattctgt tcatcctcta caacatttcg acagtctgcc actactacat     7380

gcgaatctcc ttctactact ttaccatcct gcttcacggc atggaggtgt gcgttaccat     7440

gattccctct tggctcaacg gcaagggtgc cgactacgtg tttcactcgt tcttctactg     7500

gtgcaagtgg actggagtcc acaccactgt gtatggctac gagaagaccc aggtcgaagg     7560

tcctgccgtg gtcatctgca accatcagtc ctcgctcgac attctgtcta tggcttccat     7620

ctggcccaag aactgtgttg tcatgatgaa gcggattctt gcctacgttc ccttcttcaa     7680

cctgggagcc tacttttcca acaccatctt catcgaccga tacaaccgag agcgagctat     7740

ggcttctgtc gactactgtg cctccgagat gaagaaccga aacctgaagc tctgggtgtt     7800

tcccgaaggc actcggaatc gagagggtgg attcattccc ttcaagaaag gtgccttcaa     7860

catcgctgtt cgagcccaga ttcccatcat tcctgtcgtg ttctctgact atcgagactt     7920

ctactccaag cctggccgat acttcaagaa cgatggagag gtcgtgatcc gagtcctgga     7980

tgccattccc accaagggtc tgaccctcga tgacgtctct gagctttcgg acatgtgtcg     8040

agacgtcatg ctggctgcct acaaggaagt taccctcgag gctcagcaac gaaacgccac     8100

tcgaagagga gagaccaagg acggcaagaa atccgagtaa gcggccgcat gagaagataa     8160

atatataaat acattgagat attaaatgcg ctagattaga gagcctcata ctgctcggag     8220

agaagccaag acgagtactc aaaggggatt acaccatcca tatccacaga cacaagctgg     8280

ggaaaggttc tatatacact ttccggaata ccgtagtttc cgatgttatc aatgggggca     8340

gccaggattt caggcacttc ggtgtctcgg ggtgaaatgg cgttcttggc ctccatcaag     8400

tcgtaccatg tcttcatttg cctgtcaaag taaaacagaa gcagatgaag aatgaacttg     8460

aagtgaagga atttaaatgt aacgaaactg aaatttgacc agatattgtg tccgcggtgg     8520

agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca     8580

tagctgtttc ctgtgtgaaa ttgttatccg ctcacaagct tccacacaac                8630


<210>  151
<211>  926
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (4)..(915)

<400>  151
aac atg tct gtt att gga cga ttt ctt tac tac ctg aga tcg gtg ctc         48
    Met Ser Val Ile Gly Arg Phe Leu Tyr Tyr Leu Arg Ser Val Leu           
    1               5                   10                  15            

gtc gtt ttg gcc ctc gct gga tgt ggc ttc tat ggc gtg att gcc tct         96
Val Val Leu Ala Leu Ala Gly Cys Gly Phe Tyr Gly Val Ile Ala Ser           
                20                  25                  30                

atc ctg tgt act ctc atc ggc aag cag cat ctc gcg caa tgg att acc        144
Ile Leu Cys Thr Leu Ile Gly Lys Gln His Leu Ala Gln Trp Ile Thr           
            35                  40                  45                    

gcc cga tgc ttt tac cac gtg atg aaa ctg atg ctg gga ttg gac gtc        192
Ala Arg Cys Phe Tyr His Val Met Lys Leu Met Leu Gly Leu Asp Val           
        50                  55                  60                        

aaa gtc gtg ggt gaa gag aac ctg gct aag aag ccc tat atc atg atc        240
Lys Val Val Gly Glu Glu Asn Leu Ala Lys Lys Pro Tyr Ile Met Ile           
    65                  70                  75                            

gct aac cac cag tcc acc ctc gat atc ttt atg ctc ggc aga atc ttc        288
Ala Asn His Gln Ser Thr Leu Asp Ile Phe Met Leu Gly Arg Ile Phe           
80                  85                  90                  95            

cct ccc gga tgc acc gtt acc gca aag aag agc ctt aag tac gtc ccc        336
Pro Pro Gly Cys Thr Val Thr Ala Lys Lys Ser Leu Lys Tyr Val Pro           
                100                 105                 110               

ttc ctg ggc tgg ttt atg gcg ctt tcc ggt aca tac ttc ctc gac cgt        384
Phe Leu Gly Trp Phe Met Ala Leu Ser Gly Thr Tyr Phe Leu Asp Arg           
            115                 120                 125                   

tcc aag cgg caa gag gct att gat acc ctg aac aaa gga ctg gag aac        432
Ser Lys Arg Gln Glu Ala Ile Asp Thr Leu Asn Lys Gly Leu Glu Asn           
        130                 135                 140                       

gtc aaa aag aat aag cga gcc ctc tgg gtt ttt ccc gaa ggt acc cgg        480
Val Lys Lys Asn Lys Arg Ala Leu Trp Val Phe Pro Glu Gly Thr Arg           
    145                 150                 155                           

tct tac acg tcg gag ctt acc atg ctg ccg ttc aag aag ggc gcc ttt        528
Ser Tyr Thr Ser Glu Leu Thr Met Leu Pro Phe Lys Lys Gly Ala Phe           
160                 165                 170                 175           

cat ttg gcc cag cag gga aag atc cct atc gtc cca gtg gtt gtg tct        576
His Leu Ala Gln Gln Gly Lys Ile Pro Ile Val Pro Val Val Val Ser           
                180                 185                 190               

aac act agc acg ctc gtc agc cct aag tac ggt gtg ttc aac cga ggc        624
Asn Thr Ser Thr Leu Val Ser Pro Lys Tyr Gly Val Phe Asn Arg Gly           
            195                 200                 205                   

tgt atg att gtc cga att ctg aag ccc atc tcg act gag aac ctg aca        672
Cys Met Ile Val Arg Ile Leu Lys Pro Ile Ser Thr Glu Asn Leu Thr           
        210                 215                 220                       

aaa gat aag att ggc gag ttc gct gag aaa gtg cga gat cag atg gtt        720
Lys Asp Lys Ile Gly Glu Phe Ala Glu Lys Val Arg Asp Gln Met Val           
    225                 230                 235                           

gac aca ctg aag gaa atc ggt tat tcc ccc gca atc aac gac acc acc        768
Asp Thr Leu Lys Glu Ile Gly Tyr Ser Pro Ala Ile Asn Asp Thr Thr           
240                 245                 250                 255           

ctt cct ccg cag gca att gag tac gcc gca ttg cag cat gac aag aag        816
Leu Pro Pro Gln Ala Ile Glu Tyr Ala Ala Leu Gln His Asp Lys Lys           
                260                 265                 270               

gtg aac aaa aag att aag aac gaa cct gtt cca tcg gtg tcc att tct        864
Val Asn Lys Lys Ile Lys Asn Glu Pro Val Pro Ser Val Ser Ile Ser           
            275                 280                 285                   

aat gat gtg aat act cac aac gaa gga tcg tct gtc aag aag atg cac        912
Asn Asp Val Asn Thr His Asn Glu Gly Ser Ser Val Lys Lys Met His           
        290                 295                 300                       

tag gcggccgcat g                                                       926


<210>  152
<211>  303
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  152

Met Ser Val Ile Gly Arg Phe Leu Tyr Tyr Leu Arg Ser Val Leu Val 
1               5                   10                  15      


Val Leu Ala Leu Ala Gly Cys Gly Phe Tyr Gly Val Ile Ala Ser Ile 
            20                  25                  30          


Leu Cys Thr Leu Ile Gly Lys Gln His Leu Ala Gln Trp Ile Thr Ala 
        35                  40                  45              


Arg Cys Phe Tyr His Val Met Lys Leu Met Leu Gly Leu Asp Val Lys 
    50                  55                  60                  


Val Val Gly Glu Glu Asn Leu Ala Lys Lys Pro Tyr Ile Met Ile Ala 
65                  70                  75                  80  


Asn His Gln Ser Thr Leu Asp Ile Phe Met Leu Gly Arg Ile Phe Pro 
                85                  90                  95      


Pro Gly Cys Thr Val Thr Ala Lys Lys Ser Leu Lys Tyr Val Pro Phe 
            100                 105                 110         


Leu Gly Trp Phe Met Ala Leu Ser Gly Thr Tyr Phe Leu Asp Arg Ser 
        115                 120                 125             


Lys Arg Gln Glu Ala Ile Asp Thr Leu Asn Lys Gly Leu Glu Asn Val 
    130                 135                 140                 


Lys Lys Asn Lys Arg Ala Leu Trp Val Phe Pro Glu Gly Thr Arg Ser 
145                 150                 155                 160 


Tyr Thr Ser Glu Leu Thr Met Leu Pro Phe Lys Lys Gly Ala Phe His 
                165                 170                 175     


Leu Ala Gln Gln Gly Lys Ile Pro Ile Val Pro Val Val Val Ser Asn 
            180                 185                 190         


Thr Ser Thr Leu Val Ser Pro Lys Tyr Gly Val Phe Asn Arg Gly Cys 
        195                 200                 205             


Met Ile Val Arg Ile Leu Lys Pro Ile Ser Thr Glu Asn Leu Thr Lys 
    210                 215                 220                 


Asp Lys Ile Gly Glu Phe Ala Glu Lys Val Arg Asp Gln Met Val Asp 
225                 230                 235                 240 


Thr Leu Lys Glu Ile Gly Tyr Ser Pro Ala Ile Asn Asp Thr Thr Leu 
                245                 250                 255     


Pro Pro Gln Ala Ile Glu Tyr Ala Ala Leu Gln His Asp Lys Lys Val 
            260                 265                 270         


Asn Lys Lys Ile Lys Asn Glu Pro Val Pro Ser Val Ser Ile Ser Asn 
        275                 280                 285             


Asp Val Asn Thr His Asn Glu Gly Ser Ser Val Lys Lys Met His 
    290                 295                 300             


<210>  153
<211>  7891
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY222

<400>  153
tcgaccgtac gatagttagt agacaacaat cgatagttgg agcaagggag aaatgtagag       60

tgtgaaagac tcactatggt ccgggcttat ctcgaccaat agccaaagtc tggagtttct      120

gagagaaaaa ggcaagatac gtatgtaaca aagcgacgca tggtacaata ataccggagg      180

catgtatcat agagagttag tggttcgatg atggcactgg tgcctggtat gactttatac      240

ggctgactac atatttgtcc tcagacatac aattacagtc aagcacttac ccttggacat      300

ctgtaggtac cccccggcca agacgatctc agcgtgtcgt atgtcggatt ggcgtagctc      360

cctcgctcgt caattggctc ccatctactt tcttctgctt ggctacaccc agcatgtctg      420

ctatggctcg ttttcgtgcc ttatctatcc tcccagtatt accaactcta aatgacatga      480

tgtgattggg tctacacttt catatcagag ataaggagta gcacagttgc ataaaaagcc      540

caactctaat cagcttcttc ctttcttgta attagtacaa aggtgattag cgaaatctgg      600

aagcttagtt ggccctaaaa aaatcaaaaa aagcaaaaaa cgaaaaacga aaaaccacag      660

ttttgagaac agggaggtaa cgaaggatcg tatatatata tatatatata tatacccacg      720

gatcccgaga ccggcctttg attcttccct acaaccaacc attctcacca ccctaattca      780

caaccatgtc tgttattgga cgatttcttt actacctgag atcggtgctc gtcgttttgg      840

ccctcgctgg atgtggcttc tatggcgtga ttgcctctat cctgtgtact ctcatcggca      900

agcagcatct cgcgcaatgg attaccgccc gatgctttta ccacgtgatg aaactgatgc      960

tgggattgga cgtcaaagtc gtgggtgaag agaacctggc taagaagccc tatatcatga     1020

tcgctaacca ccagtccacc ctcgatatct ttatgctcgg cagaatcttc cctcccggat     1080

gcaccgttac cgcaaagaag agccttaagt acgtcccctt cctgggctgg tttatggcgc     1140

tttccggtac atacttcctc gaccgttcca agcggcaaga ggctattgat accctgaaca     1200

aaggactgga gaacgtcaaa aagaataagc gagccctctg ggtttttccc gaaggtaccc     1260

ggtcttacac gtcggagctt accatgctgc cgttcaagaa gggcgccttt catttggccc     1320

agcagggaaa gatccctatc gtcccagtgg ttgtgtctaa cactagcacg ctcgtcagcc     1380

ctaagtacgg tgtgttcaac cgaggctgta tgattgtccg aattctgaag cccatctcga     1440

ctgagaacct gacaaaagat aagattggcg agttcgctga gaaagtgcga gatcagatgg     1500

ttgacacact gaaggaaatc ggttattccc ccgcaatcaa cgacaccacc cttcctccgc     1560

aggcaattga gtacgccgca ttgcagcatg acaagaaggt gaacaaaaag attaagaacg     1620

aacctgttcc atcggtgtcc atttctaatg atgtgaatac tcacaacgaa ggatcgtctg     1680

tcaagaagat gcactaggcg gccgcatgag aagataaata tataaataca ttgagatatt     1740

aaatgcgcta gattagagag cctcatactg ctcggagaga agccaagacg agtactcaaa     1800

ggggattaca ccatccatat ccacagacac aagctgggga aaggttctat atacactttc     1860

cggaataccg tagtttccga tgttatcaat gggggcagcc aggatttcag gcacttcggt     1920

gtctcggggt gaaatggcgt tcttggcctc catcaagtcg taccatgtct tcatttgcct     1980

gtcaaagtaa aacagaagca gatgaagaat gaacttgaag tgaaggaatt taaatgtaac     2040

gaaactgaaa tttgaccaga tattgtgtcc gcggtggagc tccagctttt gttcccttta     2100

gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg     2160

ttatccgctc acaagcttcc acacaacgta cgagccggaa gcataaagtg taaagcctgg     2220

ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag     2280

tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt     2340

ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg     2400

ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg     2460

gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag     2520

gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga     2580

cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct     2640

ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc     2700

tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg     2760

gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc     2820

tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca     2880

ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag     2940

ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct     3000

ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc     3060

accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga     3120

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca     3180

cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat     3240

taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac     3300

caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt     3360

gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt     3420

gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag     3480

ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct     3540

attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt     3600

gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc     3660

tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt     3720

agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg     3780

gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg     3840

actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct     3900

tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc     3960

attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt     4020

tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt     4080

tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg     4140

aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat     4200

tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg     4260

cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg     4320

ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct     4380

ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat     4440

cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt     4500

gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg     4560

acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac     4620

cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta     4680

aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca     4740

atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt     4800

cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc     4860

cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac     4920

tatagggcga attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata     4980

tcgaattcat gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga     5040

gactgccgag atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg     5100

ttatataata ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca     5160

ttgctaaata gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt     5220

catctcgcat tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa     5280

aatatattgt atgaacttat ttttattact tagtattatt agacaactta cttgctttat     5340

gaaaaacact tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta     5400

tgtagaataa atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata     5460

tctgcattgc ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata     5520

gtcatcgaga aatatcaact atcaaagaac agctattcac acgttactat tgagattatt     5580

attggacgag aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta     5640

tgtactattc tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct     5700

ctccaatgaa tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta     5760

gcggtatagt ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta     5820

atgatccatt aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg     5880

gctggataca taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc     5940

agtgtcaact gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga     6000

aaaaaaaaat cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta     6060

cattgttctt cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca     6120

agtacaagta catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg     6180

tttttttttg tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact     6240

tgtagtaagc cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg     6300

cgctgcgagt tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg     6360

aaatcaacgg atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct     6420

ttcttcgagc ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc     6480

cgtatcgaga aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg     6540

cagtatcata catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc     6600

tccatacttg cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta     6660

acagttaatc ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat     6720

aggatctcgg ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga     6780

catgacatcc tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc     6840

caccccgggg gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat     6900

gaagccaacc acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc     6960

gccagtggcc agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag     7020

cttctcgttg ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac     7080

gtcctccttc ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat     7140

tccggttccg ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca     7200

ccggtactgg tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa     7260

gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc     7320

gtcaatgatg tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag     7380

ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc     7440

tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc     7500

gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt     7560

tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg     7620

aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc     7680

tctctgggcg tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt     7740

gcagctgata ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc     7800

caacgaagaa tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa     7860

aggcggcaat gacgagtcag acagatactc g                                    7891


<210>  154
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 869

<400>  154
acttggcgcg ccactgccga gatccagtct ac                                     32


<210>  155
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 870

<400>  155
tcagcctagg agcatccgtt gatttccg                                          28


<210>  156
<211>  9598
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY177

<400>  156
gtacgataac ttcgtatagc atacattata cgaagttatc gcgtcgacga gtatctgtct       60

gactcgtcat tgccgccttt ggagtacgac tccaactatg agtgtgcttg gatcactttg      120

acgatacatt cttcgttgga ggctgtgggt ctgacagctg cgttttcggc gcggttggcc      180

gacaacaata tcagctgcaa cgtcattgct ggctttcatc atgatcacat ttttgtcggc      240

aaaggcgacg cccagagagc cattgacgtt ctttctaatt tggaccgata gccgtatagt      300

ccagtctatc tataagttca actaactcgt aactattacc ataacatata cttcactgcc      360

ccagataagg ttccgataaa aagttctgca gactaaattt atttcagtct cctcttcacc      420

accaaaatgc cctcctacga agctcgagct aacgtccaca agtccgcctt tgccgctcga      480

gtgctcaagc tcgtggcagc caagaaaacc aacctgtgtg cttctctgga tgttaccacc      540

accaaggagc tcattgagct tgccgataag gtcggacctt atgtgtgcat gatcaaaacc      600

catatcgaca tcattgacga cttcacctac gccggcactg tgctccccct caaggaactt      660

gctcttaagc acggtttctt cctgttcgag gacagaaagt tcgcagatat tggcaacact      720

gtcaagcacc agtaccggtg tcaccgaatc gccgagtggt ccgatatcac caacgcccac      780

ggtgtacccg gaaccggaat cattgctggc ctgcgagctg gtgccgagga aactgtctct      840

gaacagaaga aggaggacgt ctctgactac gagaactccc agtacaagga gttcctagtc      900

ccctctccca acgagaagct ggccagaggt ctgctcatgc tggccgagct gtcttgcaag      960

ggctctctgg ccactggcga gtactccaag cagaccattg agcttgcccg atccgacccc     1020

gagtttgtgg ttggcttcat tgcccagaac cgacctaagg gcgactctga ggactggctt     1080

attctgaccc ccggggtggg tcttgacgac aagggagacg ctctcggaca gcagtaccga     1140

actgttgagg atgtcatgtc taccggaacg gatatcataa ttgtcggccg aggtctgtac     1200

ggccagaacc gagatcctat tgaggaggcc aagcgatacc agaaggctgg ctgggaggct     1260

taccagaaga ttaactgtta gaggttagac tatggatatg taatttaact gtgtatatag     1320

agagcgtgca agtatggagc gcttgttcag cttgtatgat ggtcagacga cctgtctgat     1380

cgagtatgta tgatactgca caacctgtgt atccgcatga tctgtccaat ggggcatgtt     1440

gttgtgtttc tcgatacgga gatgctgggt acagtgctaa tacgttgaac tacttatact     1500

tatatgaggc tcgaagaaag ctgacttgtg tatgacttat tctcaactac atccccagtc     1560

acaataccac cactgcacta ccactacacc aaaaccatga tcaaaccacc catggacttc     1620

ctggaggcag aagaacttgt tatggaaaag ctcaagagag agatcataac ttcgtatagc     1680

atacattata cgaagttatc ctgcaggtaa aggaattcag gagagaccgg gttggcggcg     1740

tatttgtgtc ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc     1800

gggcccaacc ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac     1860

acttgccgtt aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta     1920

ctagtttctt tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa     1980

atttttttgc tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat     2040

tacctttcct cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt     2100

tccttctgag tataagaatc attcaccatg gacttcctgg aggcagaaga acttgttatg     2160

gaaaagctca agagagagaa gccaagatac tatcaagaca tgtgtcgcaa cttaattaag     2220

atgacgacat ttgcgagctg gacgaggaat agatggagcg tgtgttctga gtcgatgttt     2280

tctatggagt tgtgagtgtt agtagacatg atgggtttat atatgatgaa tgaatagatg     2340

tgattttgat ttgcacgatg gaattgagaa ctttgtaaac gtacatggga atgtatgaat     2400

gtgggggttt tgtgactgga taactgacgg tcagtggacg ccgttgttca aatatccaag     2460

agatgcgaga aactttgggt caagtgaaca tgtcctctct gttcaagtaa accatcaact     2520

atgggtagta tatttagtaa ggacaagagt tgagattctt tggagtccta gaaacgtatt     2580

ttcgcgttcc aagatcaaat tagtagagta atacgggcac gggaatccat tcatagtctc     2640

aattttccca taggtgtgct acaaggtgtt gagatgtggt acagtaccac catgattcga     2700

ggtaaagagc ccagaagtca ttgatgaggt caagaaatac acagatctac agctcaatac     2760

aatgaatatc ttctttcata ttcttcaggt gacaccaagg gtgtctattt tccccagaaa     2820

tgcgtgaaaa ggcgcgtgtg tagcgtggag tatgggttcg gttggcgtat ccttcatata     2880

tcgacgaaat agtagggcaa gagatgacaa aaagtatcta tatgtagaca gcgtagaata     2940

tggatttgat tggtataaat tcatttattg cgtgtctcac aaatactctc gataagttgg     3000

ggttaaactg gagatggaac aatgtcgata tctcgacgca tgcgacgtcg ggcccaattc     3060

gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga     3120

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg     3180

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga     3240

atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     3300

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     3360

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     3420

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     3480

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     3540

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     3600

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     3660

tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc     3720

ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc     3780

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     3840

taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc     3900

cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa     3960

acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa     4020

ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg     4080

atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa     4140

gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc     4200

acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc     4260

atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta     4320

accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag     4380

ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca     4440

acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata     4500

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     4560

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     4620

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     4680

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     4740

taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa     4800

tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt     4860

gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat     4920

cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg     4980

gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga     5040

gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac     5100

tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt     5160

ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag     5220

cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc     5280

gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag     5340

gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca     5400

gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt     5460

cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc     5520

tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc     5580

cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc     5640

cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa     5700

ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcgcgccac tgccgagatc     5760

cagtctacac tgattaattt tcgggccaat aatttaaaaa aatcgtgtta tataatatta     5820

tatgtattat atatatacat catgatgata ctgacagtca tgtcccattg ctaaatagac     5880

agactccatc tgccgcctcc aactgatgtt ctcaatattt aaggggtcat ctcgcattgt     5940

ttaataataa acagactcca tctaccgcct ccaaatgatg ttctcaaaat atattgtatg     6000

aacttatttt tattacttag tattattaga caacttactt gctttatgaa aaacacttcc     6060

tatttaggaa acaatttata atggcagttc gttcatttaa caatttatgt agaataaatg     6120

ttataaatgc gtatgggaaa tcttaaatat ggatagcata aatgatatct gcattgccta     6180

attcgaaatc aacagcaacg aaaaaaatcc cttgtacaac ataaatagtc atcgagaaat     6240

atcaactatc aaagaacagc tattcacacg ttactattga gattattatt ggacgagaat     6300

cacacactca actgtctttc tctcttctag aaatacaggt acaagtatgt actattctca     6360

ttgttcatac ttctagtcat ttcatcccac atattccttg gatttctctc caatgaatga     6420

cattctatct tgcaaattca acaattataa taagatatac caaagtagcg gtatagtggc     6480

aatcaaaaag cttctctggt gtgcttctcg tatttatttt tattctaatg atccattaaa     6540

ggtatatatt tatttcttgt tatataatcc ttttgtttat tacatgggct ggatacataa     6600

aggtattttg atttaatttt ttgcttaaat tcaatccccc ctcgttcagt gtcaactgta     6660

atggtaggaa attaccatac ttttgaagaa gcaaaaaaaa tgaaagaaaa aaaaaatcgt     6720

atttccaggt tagacgttcc gcagaatcta gaatgcggta tgcggtacat tgttcttcga     6780

acgtaaaagt tgcgctccct gagatattgt acatttttgc ttttacaagt acaagtacat     6840

cgtacaacta tgtactactg ttgatgcatc cacaacagtt tgttttgttt ttttttgttt     6900

tttttttttc taatgattca ttaccgctat gtatacctac ttgtacttgt agtaagccgg     6960

gttattggcg ttcaattaat catagactta tgaatctgca cggtgtgcgc tgcgagttac     7020

ttttagctta tgcatgctac ttgggtgtaa tattgggatc tgttcggaaa tcaacggatg     7080

ctcctaggtt cccaaaatcc cagatgcttc tctccagtgc caaaagtaag taccccacag     7140

gttttcggcc gaaaattcca cgtgcagcaa cgtcgtgtgg ggtgttaaaa tgtggggggg     7200

gggaaccagg acaagaggct cttgtgggag ccgaatgaga gcacaaagcg ggcgggtgtg     7260

ataagggcat ttttgcccat tttcccttct cctgtctctc cgacggtgat ggcgttgtgc     7320

gtcctctatt tctttttatt tctttttgtt ttatttctct gactaccgat ttggtttgat     7380

ttcctcaacc ccacacaaat aagctcgggc cgaggaatat atatatacac ggacacagtc     7440

gccctgtgga caacacgtca ctacctctac gatacacacc gtacgatagt tagtagacaa     7500

caatcgatag ttggagcaag ggagaaatgt agagtgtgaa agactcacta tggtccgggc     7560

ttatctcgac caatagccaa agtctggagt ttctgagaga aaaaggcaag atacgtatgt     7620

aacaaagcga cgcatggtac aataataccg gaggcatgta tcatagagag ttagtggttc     7680

gatgatggca ctggtgcctg gtatgacttt atacggctga ctacatattt gtcctcagac     7740

atacaattac agtcaagcac ttacccttgg acatctgtag gtaccccccg gccaagacga     7800

tctcagcgtg tcgtatgtcg gattggcgta gctccctcgc tcgtcaattg gctcccatct     7860

actttcttct gcttggctac acccagcatg tctgctatgg ctcgttttcg tgccttatct     7920

atcctcccag tattaccaac tctaaatgac atgatgtgat tgggtctaca ctttcatatc     7980

agagataagg agtagcacag ttgcataaaa agcccaactc taatcagctt cttcctttct     8040

tgtaattagt acaaaggtga ttagcgaaat ctggaagctt agttggccct aaaaaaatca     8100

aaaaaagcaa aaaacgaaaa acgaaaaacc acagttttga gaacagggag gtaacgaagg     8160

atcgtatata tatatatata tatatatacc cacggatccc gagaccggcc tttgattctt     8220

ccctacaacc aaccattctc accaccctaa ttcacaacca tgtccgttgc atccaagctc     8280

gtcttctacg tccgcgccgc catcgccgtg gtcatctttg ccgcctgtgc cacctacggc     8340

gtgctggcgt ccaccattct caccgccatc ggcaagcagg gcctggccca atggaccgtt     8400

gccagagcct tctactactc ggtgcgcatc ttcctgggta tcagcatcaa gctgcgtagc     8460

cggcaggtga ccggaaccgc cggtctggat gcctccaaga tccaggtcgc caacaccacc     8520

aagcccattg acgacatcac caaacacctg ccccgaccat gcattctgat ttccaaccac     8580

cagaacgaaa tggacattct ggtgctcggt cgcatcttcc cccagtactg ctccgtcacc     8640

gccaaaaagg ccctcaagtg gtaccctctg ctgggccagt tcatggcgct gtccggcacc     8700

atcttcctgg accgaaagga ccgaaccaag tccgtgcaga ccctcggcgg cgccgtcaag     8760

accatccaga gcggcaacgg aggcaagggc cagagcgtct tcatgttccc cgagggaacc     8820

cgatcctact ccaaggacgt cggcatcatg cccttcaaga agggctgttt ccacctggcg     8880

gtccagtcgg gcgctcccat tgtccccgtg gtggtccaga acacctcccg aatgttttct     8940

ttcggccgag gcaagctgga cgccggagag atccttgtcg acgtcctgag ccccattgag     9000

accaagggtc tggacgccag caacgtcgac gctctcatgg ccaccactta taaggccatg     9060

tgcgagactg ccgaccagat tggctacgct ggccagaaga ctcagtaggc ggccgcatga     9120

gaagataaat atataaatac attgagatat taaatgcgct agattagaga gcctcatact     9180

gctcggagag aagccaagac gagtactcaa aggggattac accatccata tccacagaca     9240

caagctgggg aaaggttcta tatacacttt ccggaatacc gtagtttccg atgttatcaa     9300

tgggggcagc caggatttca ggcacttcgg tgtctcgggg tgaaatggcg ttcttggcct     9360

ccatcaagtc gtaccatgtc ttcatttgcc tgtcaaagta aaacagaagc agatgaagaa     9420

tgaacttgaa gtgaaggaat ttaaatgtaa cgaaactgaa atttgaccag atattgtgtc     9480

cgcggtggag ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat     9540

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaagcttc cacacaac       9598


