                         SEQUENCE LISTING

<110>  ZyStor Therapeutics, Inc.
       LeBowitz, Jonathan
 
<120>  FORMULATION FOR LYSOSOMAL ENZYMES

<130>  2008266-0124 (SYM-020PCT)

<140>  NYA
<141>  2010-06-17

<150>  61/187,747
<151>  2009-06-17

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  67
<212>  PRT
<213>  Homo sapiens

<400>  1

Ala Tyr Arg Pro Ser Glu Thr Leu Cys Gly Gly Glu Leu Val Asp Thr 
1               5                   10                  15      


Leu Gln Phe Val Cys Gly Asp Arg Gly Phe Tyr Phe Ser Arg Pro Ala 
            20                  25                  30          


Ser Arg Val Ser Arg Arg Ser Arg Gly Ile Val Glu Glu Cys Cys Phe 
        35                  40                  45              


Arg Ser Cys Asp Leu Ala Leu Leu Glu Thr Tyr Cys Ala Thr Pro Ala 
    50                  55                  60                  


Lys Ser Glu 
65          


<210>  2
<211>  947
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZC-701 sequence

<400>  2

Ala Leu Cys Gly Gly Glu Leu Val Asp Thr Leu Gln Phe Val Cys Gly 
1               5                   10                  15      


Asp Arg Gly Phe Tyr Phe Ser Arg Pro Ala Ser Arg Val Ser Arg Arg 
            20                  25                  30          


Ser Arg Gly Ile Val Glu Glu Cys Cys Phe Arg Ser Cys Asp Leu Ala 
        35                  40                  45              


Leu Leu Glu Thr Tyr Cys Ala Thr Pro Ala Lys Ser Glu Gly Ala Pro 
    50                  55                  60                  


Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr Gln Cys Asp Val Pro 
65                  70                  75                  80  


Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys Ala Ile Thr Gln Glu 
                85                  90                  95      


Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro Ala Lys Gln Gly Leu 
            100                 105                 110         


Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe Phe Pro Pro Ser Tyr 
        115                 120                 125             


Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser Glu Met Gly Tyr Thr 
    130                 135                 140                 


Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe Pro Lys Asp Ile Leu 
145                 150                 155                 160 


Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu Asn Arg Leu His Phe 
                165                 170                 175     


Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu Val Pro Leu Glu Thr 
            180                 185                 190         


Pro Arg Val His Ser Arg Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe 
        195                 200                 205             


Ser Glu Glu Pro Phe Gly Val Ile Val His Arg Gln Leu Asp Gly Arg 
    210                 215                 220                 


Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe Phe Ala Asp Gln Phe 
225                 230                 235                 240 


Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala 
                245                 250                 255     


Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser Trp Thr Arg Ile Thr 
            260                 265                 270         


Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly 
        275                 280                 285             


Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly Gly Ser Ala His Gly 
    290                 295                 300                 


Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val Val Leu Gln Pro Ser 
305                 310                 315                 320 


Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile 
                325                 330                 335     


Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln Gln Tyr Leu Asp Val 
            340                 345                 350         


Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly Leu Gly Phe His Leu 
        355                 360                 365             


Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr Arg Gln Val Val Glu 
    370                 375                 380                 


Asn Met Thr Arg Ala His Phe Pro Leu Asp Val Gln Trp Asn Asp Leu 
385                 390                 395                 400 


Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe 
                405                 410                 415     


Arg Asp Phe Pro Ala Met Val Gln Glu Leu His Gln Gly Gly Arg Arg 
            420                 425                 430         


Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly 
        435                 440                 445             


Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg Gly Val Phe Ile Thr 
    450                 455                 460                 


Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val Trp Pro Gly Ser Thr 
465                 470                 475                 480 


Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp 
                485                 490                 495     


Met Val Ala Glu Phe His Asp Gln Val Pro Phe Asp Gly Met Trp Ile 
            500                 505                 510         


Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys 
        515                 520                 525             


Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val Pro Gly Val Val Gly 
    530                 535                 540                 


Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser Ser His Gln Phe Leu 
545                 550                 555                 560 


Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly Leu Thr Glu Ala Ile 
                565                 570                 575     


Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly Thr Arg Pro Phe Val 
            580                 585                 590         


Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg Tyr Ala Gly His Trp 
        595                 600                 605             


Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu Ala Ser Ser Val Pro 
    610                 615                 620                 


Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro Leu Val Gly Ala Asp 
625                 630                 635                 640 


Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu Leu Cys Val Arg Trp 
                645                 650                 655     


Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg Asn His Asn Ser Leu 
            660                 665                 670         


Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln 
        675                 680                 685             


Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala Leu Leu Pro His Leu 
    690                 695                 700                 


Tyr Thr Leu Phe His Gln Ala His Val Ala Gly Glu Thr Val Ala Arg 
705                 710                 715                 720 


Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser Thr Trp Thr Val Asp 
                725                 730                 735     


His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile Thr Pro Val Leu Gln 
            740                 745                 750         


Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr 
        755                 760                 765             


Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro 
    770                 775                 780                 


Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser Glu Gly Gln Trp Val 
785                 790                 795                 800 


Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val His Leu Arg Ala Gly 
                805                 810                 815     


Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg 
            820                 825                 830         


Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr Lys Gly Gly Glu Ala 
        835                 840                 845             


Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser Leu Glu Val Leu Glu 
    850                 855                 860                 


Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala Arg Asn Asn Thr Ile 
865                 870                 875                 880 


Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly Ala Gly Leu Gln Leu 
                885                 890                 895     


Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala Pro Gln Gln Val Leu 
            900                 905                 910         


Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr Ser Pro Asp Thr Lys 
        915                 920                 925             


Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly Glu Gln Phe Leu Val 
    930                 935                 940                 


Ser Trp Cys 
945         


<210>  3
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer sequence

<400>  3

Gly Ala Pro 
1           


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer sequence

<400>  4

Gly Gly Gly Gly Gly Pro 
1               5       


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  5

Arg Xaa Xaa Arg 
1               


<210>  6
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  X = Lys or Arg

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X = Lys or Arg

<400>  6

Xaa Xaa Xaa Xaa Xaa Arg 
1               5       


